Littérature scientifique sur le sujet « Ceratocystis platani »

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Articles de revues sur le sujet "Ceratocystis platani"

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Modafar, C. El, A. Clérivet et J. J. Macheix. « Flavan accumulation in stems of Platanus × acerifolia seedlings inoculated with Ceratocystis fimbriata f.sp. platani, the canker stain disease agent ». Canadian Journal of Botany 74, no 12 (1 décembre 1996) : 1982–87. http://dx.doi.org/10.1139/b96-237.

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Inoculation of Platanus × acerifolia stems with Ceratocystis fimbriata f.sp. platani, the canker stain disease agent, induced the accumulation of preexisting phenolic compounds such as flavan-3-ols and proanthocyanidins in the infected internode of the stem. These flavan compounds and their polymerized products were most quickly accumulated near the inoculation site, particularly in the medulla, vascular tissues, tyloses, and gels. Thus, these plane tree reactions were considered as defensive responses. Their involvement in the restriction of Ceratocystis fimbriata f.sp. platani development around the inoculation site, associated with other located responses previously described, was discussed. Keywords: Ceratocystis fimbriata, flavan-3-ols, Platanus × acerifolia, proanthocyanidins.
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Brunetti, Angela, Kurt Heungens, Jacqueline Hubert, Renaud Ioos, Gian Luca Bianchi, Francesca De Amicis, Anne Chandelier et al. « Interlaboratory Performance of a Real-Time PCR Method for Detection of Ceratocystis platani, the Agent of Canker Stain of Platanus spp. » Journal of Fungi 8, no 8 (26 juillet 2022) : 778. http://dx.doi.org/10.3390/jof8080778.

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Ceratocystis platani (CP), an ascomycetous fungus, is the agent of canker stain, a lethal vascular disease of Platanus species. Ceratocystis platani has been listed as a quarantine pest (EPPO A2 list) due to extensive damage caused in Southern Europe and the Mediterranean region. As traditional diagnostic assays are ineffective, a Real-Time PCR detection method based on EvaGreen, SYBR Green, and Taqman assays was previously developed, validated in-house, and included in the official EPPO standard PM7/14 (2). Here, we describe the results of a test performance study performed by nine European laboratories for the purpose of an interlaboratory validation. Verification of the DNA extracted from biological samples guaranteed the high quality of preparations, and the stability and the homogeneity of the aliquots intended for the laboratories. All of the laboratories reproduced nearly identical standard curves with efficiencies close to 100%. Testing of blind-coded DNA extracted from wood samples revealed that all performance parameters—diagnostic sensitivity, diagnostic specificity, accuracy and reproducibility—were best fit in most cases both at the laboratory and at the assay level. The previously established limit of detection, 3 fg per PCR reaction, was also validated with similar excellent results. The high interlaboratory performance of this Real-Time PCR method confirms its value as a primary tool to safeguard C. platani-free countries by way of an accurate monitoring, and to investigate the resistance level of potentially canker stain-resistant Platanus genotypes.
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Bernardi, R., I. Baccelli, L. Carresi, C. Comparini, L. Pazzagli et A. Scala. « Cerato-platanin elicits transcription of defence-related genes earlier than Ceratocystis platani on Platanus acerifolia ». Forest Pathology 41, no 4 (23 juin 2010) : 255–61. http://dx.doi.org/10.1111/j.1439-0329.2010.00668.x.

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Luchi, Nicola, Luisa Ghelardini, Lassaâd Belbahri, Marion Quartier et Alberto Santini. « Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay ». Applied and Environmental Microbiology 79, no 17 (28 juin 2013) : 5394–404. http://dx.doi.org/10.1128/aem.01484-13.

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ABSTRACTCeratocystis plataniis the causal agent of canker stain of plane trees, a lethal disease able to kill mature trees in one or two successive growing seasons. The pathogen is a quarantine organism and has a negative impact on anthropogenic and natural populations of plane trees. Contaminated sawdust produced during pruning and sanitation fellings can contribute to disease spread. The goal of this study was to design a rapid, real-time quantitative PCR assay to detect aC. plataniairborne inoculum. Airborne inoculum traps (AITs) were placed in an urban setting in the city of Florence, Italy, where the disease was present. Primers and TaqMan minor groove binder (MGB) probes were designed to target cerato-platanin (CP) and internal transcribed spacer 2 (ITS2) genes. The detection limits of the assay were 0.05 pg/μl and 2 fg/μl of fungal DNA for CP and ITS, respectively. Pathogen detection directly from AITs demonstrated specificity and high sensitivity forC. platani, detecting DNA concentrations as low as 1.2 × 10−2to 1.4 × 10−2pg/μl, corresponding to ∼10 conidia per ml. Airborne inoculum traps were able to detect theC. plataniinoculum within 200 m of the closest symptomatic infected plane tree. The combination of airborne trapping and real-time quantitative PCR assay provides a rapid and sensitive method for the specific detection of aC. plataniinoculum. This technique may be used to identify the period of highest risk of pathogen spread in a site, thus helping disease management.
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Grosclaude, C., R. Olivier, J. C. Pizzuto, Corinne Romiti et Sylvie Madec. « Détection par piégeage du Ceratocystis fimbriata f. platani ». European Journal of Forest Pathology 18, no 7 (décembre 1988) : 385–90. http://dx.doi.org/10.1111/j.1439-0329.1988.tb00226.x.

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Clerivet, A., et C. Modafar. « Vascular modifications in Platanus acerifolia seedlings inoculated with Ceratocystis fimbriata f. sp. platani ». Forest Pathology 24, no 1 (mars 1994) : 1–10. http://dx.doi.org/10.1111/j.1439-0329.1994.tb01317.x.

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Lehtijärvi, A., F. Oskay, H. T. Doğmuş Lehtijärvi, A. G. Aday Kaya, F. Pecori, A. Santini et S. Woodward. « Ceratocystis platani is killing plane trees in Istanbul (Turkey) ». Forest Pathology 48, no 1 (1 août 2017) : e12375. http://dx.doi.org/10.1111/efp.12375.

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Ocasio-Morales, Roberto G., Panaghiotis Tsopelas et Thomas C. Harrington. « Origin of Ceratocystis platani on Native Platanus orientalis in Greece and Its Impact on Natural Forests ». Plant Disease 91, no 7 (juillet 2007) : 901–4. http://dx.doi.org/10.1094/pdis-91-7-0901.

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Canker stain of plane tree recently was reported in a small area of southwestern Greece on natural populations of the important riparian species, oriental plane tree, Platanus orientalis. The fungus Ceratocystis platani (= C. fimbriata f. platani) was successfully isolated from infected, stained wood of 15 dead or dying trees on the Peloponnese Peninsula. Genetic analyses of these 15 isolates from Greece, using nuclear and mitochondrial DNA fingerprints, showed the fungus to be identical to the genotype reported from Italy, France, and Switzerland. A polymerase chain reaction-based microsatellite analysis of eight polymorphic loci discovered a new microsatellite allele in one of the isolates from Greece, but this may be due to a mutation after introduction of a single strain. Earlier studies indicated that the most common European genotype had been introduced from eastern North America to Italy during World War II. The recent introduction to Greece appears to have originated from Italy, France, or Switzerland, rather than from eastern North America, where the fungus is native. The pathogen is having a dramatic impact on the natural population of P. orientalis in southwestern Greece, and containment measures should be imposed before it spreads throughout the natural range of this ecologically and historically important host.
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Pilotti, M., G. Di Lernia, V. Modesti, V. Lumia et A. Brunetti. « Outcome of Ceratocystis platani inoculations in Platanus × acerifolia in relation to season and inoculum dose ». iForest - Biogeosciences and Forestry 9, no 4 (9 août 2016) : 608–17. http://dx.doi.org/10.3832/ifor1594-008.

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El Modafar, C., A. Clerivet, A. Fleuriet et J. J. Macheix. « Inoculation of Platanus acerifolia with Ceratocystis fimbriata F. Sp. Platani induces scopoletin and umbelliferone accumulation ». Phytochemistry 34, no 5 (novembre 1993) : 1271–76. http://dx.doi.org/10.1016/0031-9422(91)80014-r.

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Thèses sur le sujet "Ceratocystis platani"

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Diop-Bruckler, Marguerite. « Détection précoce de Ceratocystis fimbriata F. Platani, agent du chancre coloré du platane, par le test Elisa ». Montpellier 2, 1991. http://www.theses.fr/1991MON20020.

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L'objectif de ce travail est de trouver un moyen de diagnostic precoce par le test elisa, de ceratocystis fimbriata f. Platani, agent du chancre colore du platane. Cette grave maladie sevit dans plusieurs pays du sud de l'europe, dont le sud-est de la france. Un antiserum est fabrique avec les extraits proteiques de l'isolat de c. Fimbriata entr2. Il reconnait l'antigene homologue, ainsi que d'autres isolats, au total 10, provenant de suisse ou de marseille. L'antiserum ne reconnait aucun de champignons lignivores couramment rencontres dans notre region sur platane. Sa confrontation avec 12 especes de ceratocystis issues de diverses essences forestieres ne montre de parente serologique qu'avec c. Olivacea. Donc on peut dire que l'antiserum est dote d'un bon niveau de specificite et un spectre de detection large. Dans le cas de bois peu contamine, la mise en evidence de c. Fimbriata f. Platani n'est possible que par le test elisa. Une amplification des reponses est etudiee avec le systeme biotine-avidine qui permet une hausse significative des densites optiques. Des techniques tres fines, empruntees a la geostatistique, sont etudiees pour apprecier quantitativement la repartition spatiale du parasite au sein des arbres infectes. Cette demarche indispensable a permis de raisonner un mode d'echantillonnage optimal au sein des arbres apparemment sains. C. Fimbriata f. Platani peut etre detecte par ses aromes dont la composante principale est l'acetate d'isobutyle. La validation de cette technique doit etre poursuivie sur des echantillons de bois de platane provenant d'arbres apparemment sains
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Matasci-Stanga, Maria. « Ceratocystis fimbriata Ell. et Halsted f.sp. Platani Walter : evoluzione e gestione dell'epidemia nel cantone Ticino / ». [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10350.

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LUTI, SIMONE. « CP from Ceratocystis platani : primary role in the fungal life and functions in interaction with plants ». Doctoral thesis, 2014. http://hdl.handle.net/2158/851106.

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Aglietti, Chiara, Paolo Capretti, Nicola Luchi, Luisa Ghelardini et Alberto Santini. « Messa a punto di tecniche diagnostiche per patogeni di quarantena/Development of diagnostics techniques for studying quarantine plant pathogens ». Doctoral thesis, 2020. http://hdl.handle.net/2158/1187844.

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The aim of this thesis was to optimize and improving reliable, fast, sensitive and specific field-deployable tools for the early detection of quarantine plant pathogens. In the first part of the thesis the work was concentrated in developing a field-applicable LAMP-based assay for the detection of Xylella fastidiosa, Phytophthora ramorum and Ceratocystis platani. Each assay, optimized on the portable instrument Genie II ® (Optigene, UK), was based on the conventional LAMP reaction and showed the capability to detect X. fastidiosa, C. platani and P. ramorum with high specificity and sensitivity in only 30 minutes also on plant samples for which a rapid kit method for in field-DNA extraction was also utilized. However, the assay targeting C. platani and P. ramorum were able to detect also C. fimbriata and P. lateralis, having also many cross reactions with other Phythophtora species. Even if the specificity was assessed by results obtained from melting analyses, that gave different temperature between target and non-target species, improving the specificity of a LAMP assay was needed. The second part of the thesis was hence concentrated in improving the chemistry and the specificity of a LAMP assay. The use of sequence-specific LAMP probes was analyzed by the development of a conventional and FRET-assimilating probe-based LAMP method targeting Fusarium circinatum, a pine pathogen for which specificity is a very important requirement concerning diagnostics. The capability of increasing the specificity using this novel LAMP chemistry was assessed by comparing LAMP results of conventional and probe-based LAMP reaction developed for F. circinatum: with conventional reaction many cross reactions were obtained with phylogenetically closest Fusaria while with the probe-based method only F. temperatum was amplified as cross reaction. Due to positive results obtained applying the probe-based method on wood samples DNA extracted with the field method the suitability for using it into the field was also assessed. The same probe-based LAMP chemistry was then implemented for multiplex application concerning pine needles pathogens Dothistroma septosporum, Dothistroma pini and Lecanosticta acicola, obtaining as preliminary results that of having a multiplex specific reaction directly in the field in about 10 minutes. Concerning this third part of this work, the possibility to apply the described method on crude samples was investigated concerning pine needles for which preliminary test to optimize a field suitable crude extraction method were carried out with promising results. As in the last part of this work was assessed that in Italy the distribution of Dothistroma septosporum is widespread by applying a TaqMan-based qPCR method while L. acicola was reported only in restricted places and D. pini was never reported, the developed LAMP method could be useful to prevent and monitoring their spread and introduction.
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Chapitres de livres sur le sujet "Ceratocystis platani"

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Clerivet, A., et C. El Modafar. « Host-Parasite Interactions (Platanus Acerifolia Willt – Ceratocystis Fimbriata F. Sp. Platani [E. and H.] Walter). Vascular Reactions Related to Host Defense ». Dans Developments in Plant Pathology, 379. Dordrecht : Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1737-1_115.

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