Littérature scientifique sur le sujet « Cellules stromales mésenchymateuses – Cultures cellulaires »
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Articles de revues sur le sujet "Cellules stromales mésenchymateuses – Cultures cellulaires"
Borhan, R., J. Lee, G. Youssef, A. Bensussan et M. Philpott. « Différence de signature génétique moléculaire des cellules du derme papillaire entre le cuir chevelu alopécique et le cuir chevelu non alopécique dans les cultures cellulaires bidimensionnelle, tridimensionnelle et co-culture avec les cellules souches mésenchymateuses dérivées des adipocytes ». Annales de Dermatologie et de Vénéréologie 146, no 12 (décembre 2019) : A247. http://dx.doi.org/10.1016/j.annder.2019.09.388.
Texte intégralThèses sur le sujet "Cellules stromales mésenchymateuses – Cultures cellulaires"
Magne, Brice. « Évaluation d’une stratégie de préconditionnement de cellules stromales mésenchymateuses pour le traitement des grands-brulés ». Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS558/document.
Texte intégralSince the 1980’s, little progress has been made in the management of major burns, in spite of several research advances in the field of skin tissue engineering and regenerative medicine. Developed in 1975, Cultured Epithelial Autografts (CEA) are the last-in-date significant breakthrough, allowing patient survival in most critical cases. However, patients still have to cope with debilitating sequelae including hypertrophic scars, skin fragility, immunometabolic dysfunctions, chronic pain and post-traumatic stress disorder. Mesenchymal Stromal Cells (MSC) have raised an increasing interest during the past 50 years due to their trophic and immunomodulatory properties. Recent findings about their high plasticity to external stimuli have fostered the development of new targeted therapies known as “preconditioning strategies”. This PhD work thus aimed to assess a MSC preconditioning strategy for the treatment of major burns using CEA. The present work was divided into three main experimental parts. First, in vitro experiments were developed in order to set up the preconditioning strategy, and revealed the interesting role of both interleukin-1β (IL-1β) and substance P (SP). Then, both effectiveness and mechanism of action of these preconditioning modalities were assessed in vitro and in vivo, either using MSC or their secretory products. It was thus shown that IL-1β could potentiate MSC effectiveness through the promotion of pro-migratory, anti-inflammatory and pro-remodeling activities. This effect was shown to be partly mediated by the TGF-β1 signaling pathway. At last, this preconditioning strategy was evaluated in a third degree burn rat model mimicking the surgical treatment applied to severe burn patients. Despite technical hurdles limiting CEA engraftment, anti-inflammatory and pro-angiogenic properties of MSCs were confirmed in this model. These preliminary results underline the potential of a preconditioned MSC therapy in wound healing. Additional preclinical studies are now required to corroborate the benefit of such a therapy in major burns
Ishac, Nicole. « Comment deux lignées cellulaires stromales mésenchymateuses humaines récapitulent in vitro le microenvironnement hématopoïétique ? : Intérêt en ingénierie ». Thesis, Tours, 2015. http://www.theses.fr/2015TOUR4038/document.
Texte intégralHematopoiesis occurs in a hypoxic microenvironment or niche in which hematopoietic stem cells (HSCs) are in close contact with mesenchymal stromal cells. Cellular interactions as well as microenvironmental factors such as reactive oxygen species are crucial for the maintenance of normal and leukemic HSCs. Developing an in vitro human culture system that closely mimcs marrow physiology is therefore essential to study the niche. Here, we present a model using two human stromal cell lines, HS-27a and HS-5. Previously poorly described in the literature, we have further characterized both of these cell lines. The first objective was to assess the quality of HS-27a and HS-5 niches by investigating their cellular, molecular and functional characteristics. Our results clearly show that HS-27a cells display features of a “quiescent” niche whereas HS-5 cells rather represent a “proliferative” niche. The second objective was to engineer a hematopoietic niche where the oxidative metabolism is optimized for the expression of an antioxidant protein, glutathione peroxidase 3 (GPx3). The originality of this work is the use of a non-viral gene transfer system by using the transposon piggyBac. This strategy was achieved by delivering a DNA plasmid carrying the gene of interest, and an mRNA source of transposase, the enzyme which catalyzes the transgene integration. Functionally, GPx3 was shown to be a key regulator for sustaining hematopoietic homeostasis by maintaining immature progenitor cells. For the first time, an original non-viral gene transfer has been used to create an in vitro hematopoietic niche that recapitulates the complexity of normal and leukemic microenvironment. This niche not only provides a platform to identify regulatory factors controlling medullary cells, but may also help in the development of targeted therapeutic strategies
Maillot, Charlotte. « Quantification and impact of microcarrier collisions during mesenchymal stem cell culture in bioreactors ». Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0314.
Texte intégralTo date, bottlenecks persist concerning deep scientific understanding of how various process parameters will impact the Mesenchymal Stem Cell production. Specifically applied to microcarrier-based expansion processes of WJ-MSC's, very little information is available to characterize the impact of microcarrier concentration on MSC growth and death rates or on critical quality attributes which may have crucial and possibly dangerous clinical impacts. As a result, the following work proposes to rationally describe the impact of particle concentration on MSC growth through a pluri-disciplinary characterization of microcarrier-microcarrier interactions in agitated conditions. In order to do so the biological and physical aspects of this work will be presented. To begin with, a quantitative approach to estimate cell growth and death kinetics caused by microcarrier-microcarrier collisions in both Erlenmeyer Flasks and Spinner Flasks is described. For this, cells were grown at various microcarrier concentrations using two microcarrier types : Cytodex-1 and Synthemax II. Complementary cultures were performed by adding various concentrations of particles with the same size and density as microcarriers in view of providing specific information on how additional particles may impact MSC growth on microcarriers. In addition, elements of MSC characterization were performed for these experiments to understand not only the impact of microcarrier-microcarrier interactions on growth but also on defined elements of cell quality. In parallel, in order to estimate the amount and intensity of microcarrier-microcarrier collisions in a specific tank geometry, experiments were performed using both the attenuation of light by Cytodex-1 microcarriers (to estimate local microcarrier concentration) and the acoustic signal which comes from particles colliding with a hydrophone (to estimate microcarrier-sensor collision frequency and intensity). These experiments provided elements to estimate the amount of particle collisions that MSC's may perceive during specific dynamic and steady phases of cell culture in STR's. Lastly, a bioreactor-based approach to MSC manufacturing will be presented focusing on biological aspects of how particle concentration and agitation impacts MSC growth and quality attributes. For this, various MSC cultures were performed in STRs with varying particle concentrations and agitation strategies. The MSC's produced in these conditions were then characterized to define if certain critical quality attributes could be affected by parameters such as microcarrier concentration and/or agitation
Loncaric, Darija. « Atténuation des oxydations phosphorylantes et induction d'une réponse cellulaire hypoxique : effêt de l'[alpha]-tocophérol-acétate et de miR-210 sur les cellules stromales mesenchymateuses ». Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0198.
Texte intégralIn this thesis, we combined approaches of single-cell cultures, flow-cytometry, energetic metabolismanalysis and molecular genetics in order to get insight in the effects of α-Tocopherol-Acetate (α-TOA)on Mesenchymal Stromal Cells (MStroC) and their functional subpopulations (mesenchymal stem and progenitor cells). The other aim was to test a miR-210 molecule with respect to its potential use as hypoxia mimicking molecule. After defining MStroC population heterogeneity and concluding that the first passage population is convenient for further experiments, we demonstrated that α-TOA exhibits a positive effect on the maintenance of high proliferative capacity of mesenchymal stem cells. This effect could be associated with an attenuation of electron transport chain (ETC) activity, which, on the other hand could explain moderate increase in the level of mitochondrial Reactive Oxygen Species (mtROS) we detected. The increase in mtROS level could be associated with a decreased HIF-1 alpha protein degradation in MStroC exposed to α-TOA. Although we did not detect a compensatory increase in glycolysis, the observed phenomena depict part of a complex cellular response to the low O2 that is demonstrated to be related with maintenance of stem cell primitiveness. The exact mechanism remains to be elucidated as well as its translational potential. In addition, we provided new evidences that miR-210 is integral part in MStroC response to low O2. In the study, we showed increased in miR-210 expression in a short-term (up to 24 hours) and after extended (up to 72 hours) MStroC exposed to low O2. Moreover, we demonstrated that this micro- RNA could be regulated by both HIF-1 and HIF-2 transcriptional factors, suggesting it as integral part of MStroC response to low O2. So far, our data suggest that miR-210 is worthy to be considered as good hypoxia mimicking molecule
Martin, Céline. « Étude des procédés d’amplification de cellules souches mésenchymateuses humaines ». Thesis, Université de Lorraine, 2016. http://www.theses.fr/2016LORR0261/document.
Texte intégralProgress in regenerative medicines over the past ten years have led to an important research mobilisation, but obtaining a sufficient amount of human stem cells remains nonetheless problematic, especially for mesenchymal stem cells (MSC). Hence, this work developed an approach coupling biology and process engineering to identify barriers limiting MSC growth. The study of scaled-up amplification methods was performed using microcarriers and a 200~mL minibioreactors platform. In order to maximise MSC growth in a biochemically controlled environment, a serum free medium development was tested as well. Human MSC as model cell type for cellular therapies have thus been demonstrated as extremely sensitive to freeze/thaw cycles, temperature variations, subject to premature aging and needing a complex medium enriched in multiple growth and adherence factors. Following this study, several pitfalls might be avoided during MSC process scale-up by integrating the cells biology into the bioreactors' process engineering parameters (heat transfer, hydrodamic stress, adhesion surface)
Sion, Caroline. « Development of an optimized perfused-continuous process of culture of human umbilical cord mesenchymal stem cells (hMSC) grown on innovative adhesion supports ». Electronic Thesis or Diss., Université de Lorraine, 2020. http://www.theses.fr/2020LORR0113.
Texte intégralMesenchymal stem cells (MSCs) show great interest in cellular therapies. Their various characteristics such as their immuno-modulatory properties, their ability to differentiate, and also the secretion of factors, are numerous and promising for new clinical treatments for diseases where few therapies are proposed or have few efficiencies. The doses to be injected for significant results must be repeated and generally contain high quantities of cells (106 cells kg-1 per patient approx). Large scale production methods must be implemented to meet the demand, and in the least costly way possible. In this PhD work, the main objective was to develop a scalable process adapted to these support-dependent cells. For this end, a first study allowed to understand part of the mechanisms of interaction of cells with their growth supports, the microcarriers. The adhesion time but also the cell migrations between microcarriers were characterized and evaluated. A strategy of fed-batch mode strategy with microcarriers addition at specific times in the culture was also proposed. Following this, the second part of the study of this work was to determine the efficiency on larger scale expansion process (1.5 L), using of innovative microcarriers developed by the partner teams of the ‘ImprovesStem’ European project. Several microcarriers candidates with chemically modified surface proved to be promising for the expansion of Wharton’s jelly stem cells. Finally, in the last part of the thesis, an innovative process based on the removal of empty microcarriers, avoiding the risk of deleterious frictions between highly concentrated microcarriers was proposed. Moreover, an on-line monitoring of viable cell concentration was carried-out in the stirred tank bioreactor. Innovative commercial microcarriers, soluble under the action of enzymes, were used in this last part of the study. An improvement of the expansion factor (by a factor of 1.5) was obtained in this continuous-perfused mode of culture in the stirred bioreactor. In addition, these enzymatically-soluble commercial microcarriers allowed for an excellent detachment yield, essential to consider their use in cell therapy
Le, Pape Fiona. « Evaluation de la contribution d'une hémoglobine marine dans la culture cellulaire et dans la cellularisation de substituts osseux et méniscaux par des cellules souches mésenchymateuses ». Thesis, Brest, 2016. http://www.theses.fr/2016BRES0002/document.
Texte intégralThis work aimed to develop cell culture systems, in 2D and 3D, based on the properties of HEMOXCell®, a marine oxygen carrier. Our approach was articulated in two main parts: the first one dealing with the assessment of the use of HEMOXCell® in the culture of two cellular models, and the second one, exploiting the results obtained for tissue engineering purposes. In this first axis, the dose-response effect of HEMOXCell® in the CHO-S cells and mesenchymal stem cells (MSC) in vitro culture, allowed the identification of optimal working concentrations, which can promote cell viability and proliferation. The CHO-S model has contributed to the establishment of a performance test of the molecule, and encouraged its use for bioproduction stimulation. The tests performed on MSCs were used to validate the harmlessness of the molecule at low doses and the maintenance of "stemness". The idea to associate MSCs with porous scaffolds is a promising approach for tissue engineering applications, but it is confronted to the lack of oxygen in the depth of the substitutes. In the second part of this project, we worked at improving the cellularization of bone and meniscal substitutes, under static and dynamic culture systems, w/ and w/o HEMOXCell®. In parallel, a study was conducted to attempt to characterize the meniscal cells. Analyses of cellularized biomaterials suggest a beneficial effect of HEMOXCell® when used as a differentiation media supplement. This work contributed to improve this oxygen carrier understanding and to extend the field of its potential uses particularly for therapeutic applications
Albert, Philippe. « Contribution de la culture de cellules à l'étude du contrôle de la prolifération cellulaire au cours de la régénération du membre d'Axolotl (Amphibien Urodèle) ». Lille 1, 1987. http://www.theses.fr/1987LIL10038.
Texte intégralRoger, Mathilde. « Cellules stromales mésenchymateuses comme vecteurs cellulaires de nanoparticules : un nouvel outil thérapeutique des tumeurs cérébrales ». Phd thesis, Université d'Angers, 2010. http://tel.archives-ouvertes.fr/tel-00577731.
Texte intégralMazurier, Christelle. « Perspectives de thérapies cellulaires dans le myélome multiple ». Versailles-St Quentin en Yvelines, 2013. http://www.theses.fr/2013VERS0054.
Texte intégralMultiple Myeloma (MM) is a frequent haematological disorder, often incurable, characterized by a clonal plasma cell proliferation, anemia, kidney insufficiency and hypercalcemy. Current treatments are based on the administration of chemotherapies followed by autologous or allogenic hematopoietic stem cells transplantation. My work is a translational research work which finally aims at developing new cell therapy protocols. First, I have studied the feasability of developing autologous anti-tumoral vaccination to eradicate the plasma cell development. Secondly I have analyzed the putative role of Mesenchymal stem cells (MSC) in osteolysis. Finally, I have focused on mechanisms responsible of immunosuppressive properties of MSC to use them as accessory cells to attenuate the toxicity of the allograft