Thèses sur le sujet « Cell adhesion and migration »
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Burthem, John. « Hairy cell adhesion and migration ». Thesis, University of Liverpool, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240394.
Texte intégralChen, Ning. « Role of cell adhesion molecules in melanoma transendothelial migration ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ58734.pdf.
Texte intégralBaghdadchi, Negin. « CYTOKINE CONTROL OF GLIOMA ADHESION AND MIGRATION ». CSUSB ScholarWorks, 2014. https://scholarworks.lib.csusb.edu/etd/93.
Texte intégralChon, John H. « Mediation of vascular smooth muscle cell adhesion and migration by cell surface heparan sulfate glycosaminoglycans ». Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/11315.
Texte intégralLi, ShuShun. « Thrombospondin 1, an autocrine regulator in T cell adhesion and migration ». Doctoral thesis, Umeå : Klinisk mikrobiologi, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-599.
Texte intégralVielkind, Susina. « Role of the GTPase Rho in T cell adhesion and migration ». Thesis, University College London (University of London), 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406052.
Texte intégralRabut, Anne. « Regulation of Drosophila E-cadherin mediated adhesion during border cell migration ». Université Louis Pasteur (Strasbourg) (1971-2008), 2004. https://publication-theses.unistra.fr/public/theses_doctorat/2004/RABUT_Anne_2004.pdf.
Texte intégralClassic cadherins are major mediators of homophilic cell-cell adhesion during animal development. In many developmental processes cadherin-dependent adhesive interactions between cells are likely to be regulated. A lot has been done to understand how adhesion is regulated in tissue culture experiments, but so far little is known about the relevance of the studied regulatory mechanisms in vivo. We used Drosophila as a model to study these putative regulatory mechanisms during development. Several mutant variants of Drosophila Epithelial cadherin (DE-cadherin) were generated. Their ability to substitute for endogenous DE-cadherin activity was analyzed in multiple cadherin-dependent processes during Drosophila development and oogenesis, in particular during border cell migration, a process that probably requires dynamic adhesion. Using different DE-cadherin variants, I showed that DE-cadherin/p120ctn interaction, DE-cadherin juxtamembrane domain as well as the conserved tyrosines in DE-cadherin cytoplasmic domain are surprisingly all dispensable for DE-cadherin function. DE-cadherin-Db/a-catenin (a-catenin fused after DE-cadherin juxtamembrane domain) partially substitutes for endogenous DE-cadherin in border cells, showing that regulation of the link between DE-cadherin and a-catenin is not strictly required for the migration. DE-cadherin-DCyt/a-catenin (a-catenin fused after DE-cadherin transmembrane domain) is able to mediate adhesion in the follicular epithelium but does not substitute for endogenous DE-cadherin in border cells, suggesting that some regulatory signals important for the migration may be located in DE-cadherin cytoplasmic domain. However, DE-cadherin-DCyt/a-catenin also have some clear subcellular localization defects and it is so far not completely clear if the observed migration defects are really due to lack of adhesion regulation. If this is the case, the precise regulatory mechanisms will still need to be identified
Lee, Eun-ju Yousaf Muhammad. « Development of dynamic substrates for studies of cell adhesion and migration ». Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2009. http://dc.lib.unc.edu/u?/etd,2284.
Texte intégralTitle from electronic title page (viewed Jun. 26, 2009). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Chemistry." Discipline: Chemistry; Department/School: Chemistry.
Bliss, Katherine Theresa. « Elucidating the Role of Lasp-2 in Cell Adhesion and Migration ». Diss., The University of Arizona, 2012. http://hdl.handle.net/10150/255198.
Texte intégralFERRARIS, G. M. SARRA. « NON-INTEGRIN CELL ADHESION TRIGGERS LIGAND-INDEPENDENT INTEGRIN SIGNALING ». Doctoral thesis, Università degli Studi di Milano, 2011. http://hdl.handle.net/2434/157459.
Texte intégralTanizaki, Hideaki. « Rho-mDia1 pathway is required for adhesion, migration, and T cell stimulation in dendritic cells ». Kyoto University, 2011. http://hdl.handle.net/2433/142071.
Texte intégralMangiante, Lee Elena Taylor Joan M. « The role of focal adhesion kinase in vascular smooth muscle cell migration ». Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2008. http://dc.lib.unc.edu/u?/etd,2137.
Texte intégralTitle from electronic title page (viewed Feb. 17, 2009). "... in partial fulfillment of the requirements for the degree of Master of Science in the Department of Pathology and Laboratory Medicine." Discipline: Pathology and Laboratory Medicine; Department/School: Medicine.
Sundar, Rajan Vinoth Edal Joseph. « Adhesion and transendothelial migration of cancer cells ». Thesis, Université Grenoble Alpes (ComUE), 2016. http://www.theses.fr/2016GREAV065/document.
Texte intégralCancer metastasis is associated with 90% cancer-associated deaths, when cancer cells escape from the primary tumor and form metastatic colonies in secondary sites. Extravasation is an important step in cancer metastasis, where cancer cells carried in blood, adhere and transmigrate through the endothelium. Therefore identifying the key molecules involved during the adhesion process could enable to develop new anticancer cancer drugs able to inhibit the adhesion of cancer cells to the endothelium. We have previously shown that InterCellular Adhesion Molecule-1 (ICAM-1) expressed by endothelial cells is involved in the interactions of bladder cancer cells (BCs) with the endothelium. However the ICAM-1 ligands have never been investigated. In this study, we combined adhesion assays and Atomic Force Microscopy (AFM) to identify the ligands involved and to quantify the forces relevant in such interactions. We report the expression of MUC1 and CD43 on BCs and demonstrate that these ligands interact with ICAM-1 to mediate cancer cell-endothelial cell adhesion in the case of the more invasive BCs. AFM experiments were performed to quantify the force ranges involved by MUC1 and CD43 during their interaction with ICAM-1. AFM measurements combined with a Gaussian Mixture Model showed distinct force ranges for the interaction of ICAM-1 with MUC1 and ICAM-1 with CD43. Furthermore, a detailed analysis of the rupture events suggests that CD43 is strongly connected to the cytoskeleton and that its interaction with ICAM-1 mainly corresponds to force ramps followed by sudden jumps. On the contrary, MUC1 seems to be weakly connected to the cytoskeleton as its interactions with ICAM-1 are mainly associated with the formation of tethers. The forces involved during the transmigration of cancer cells through the endothelium was investigated using Traction Force Microscopy (TFM). Preliminary results showed that tractions exerted by cancer cells during transmigration can be studied and quantified using TFM
Hu, Shiqiong. « Mechanics and Dynamics of Cell Adhesion : Experimental Study of the Osteoclasts ». Lyon, Ecole normale supérieure, 2010. http://www.theses.fr/2010ENSL0594.
Texte intégralLes ostéoclastes sont des cellules multinucléées, responsables de la résorption osseuse. Quand ils sont déposés sur un substrat, des structures ponctuelles riches en actine, les podosomes, apparaissent et s'assemblent en clusters, anneaux ou ceinture. Nous avons étudié expérimentalement leur fonction et leur dynamique, depuis une population entière jusqu'à l'échelle d'un unique podosome. Sur une population de cellules, des mesures cinétiques montrent que la surface de la cellule A varie comme A ~ K2, où K est le nombre de noyaux ; ce résultat indique une forme aplatie. Par ailleurs, la mesure de quantités qui prennent en compte l'organisation spatiale de l'actine dans la cellule montre que l'organisation des podosomes ne dépend que du temps écoulé après différentiation, et non de K. Dans un seul ostéoclaste, l'observation d'un fort couplage entre l'étalement d'une cellule et la formation des podosomes nous a conduit a suggérer que les podosomes jouent un rôle important dans la mobilité des ostéoclastes. L'analyse de la migration d'ostéoclastes, ainsi que des forces appliquées sur le substrat, montre que la dynamique interne de l'actine dans la cellule est non seulement corrélée avec la migration cellulaire, mais la gouverne. Enfin, afin de comprendre la dynamique interne d'un podosome, nous avons amélioré le modèle de Biben et al. (2005), en prenant en compte d'une part, la polymérisation de l'actine, et d'autre part, la diffusion et la cinétique d'attachement de la gelsoline, une protéine responsable de la coupe des filaments d'actine. Nous montrons que les podosomes sont principalement gouvernés par la dynamique de l'actine, indépendamment de la concentration en gelsoline
Tse, Kathy Wan-Kei. « The role of Pyk2 and FAK in B cell migration, adhesion, and spreading ». Thesis, University of British Columbia, 2010. http://hdl.handle.net/2429/25041.
Texte intégralSroka, Thomas Charles. « Synthetic Peptide Ligand Mimetics and Tumor Cell Motility ». Diss., Tucson, Arizona : University of Arizona, 2005. http://etd.library.arizona.edu/etd/GetFileServlet?file=file:///data1/pdf/etd/azu%5Fetd%5F1325%5F1%5Fm.pdf&type=application/pdf.
Texte intégralSoghomonians, Arlen. « Macaque trophoblast migration and cell interactions : potential role of shear stress, cell adhesion molecules and cigarette smoke / ». For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.
Texte intégralFerro, Valerie Anne. « The role of endothelial cells in promoting adhesion, spreading and migration of B16F10 cells ». Thesis, University of St Andrews, 1989. http://hdl.handle.net/10023/14067.
Texte intégralChon, John H. « Characterization of single-cell movement using a computer-aided fluorescence time-lapse videomicroscopy system : role of integrins in endothelial cell migration ». Thesis, Georgia Institute of Technology, 1996. http://hdl.handle.net/1853/11171.
Texte intégralEssex, Rachel R. « Determining the Effects of CD151 and β1 on Tumor Cell Adhesion and Migration ». UKnowledge, 2015. http://uknowledge.uky.edu/cme_etds/56.
Texte intégralGolé, Laurent. « Migration of Dictyostelium Amoeba : role of Adhesion and Quorum sensing ». Phd thesis, Université Claude Bernard - Lyon I, 2011. http://tel.archives-ouvertes.fr/tel-00846586.
Texte intégralEiseler, Tim. « Role of protein kinase D (PKD) in migration, invasion and cell adhesion of pancreas ductal adenocarcinoma cells ». [S.l. : s.n.], 2006. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-26245.
Texte intégralEl-Hariry, Iman Ahmed. « The relationship between FGF/FGFR and E-cadherin/catenin systems in pancreatic adenocarcinoma ». Thesis, Imperial College London, 1999. http://hdl.handle.net/10044/1/7552.
Texte intégralJennifer, Leigh Quizi. « SLK-mediated Phosphorylation of Paxillin Is Required for Focal Adhesion Turnover and Cell Migration ». Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20483.
Texte intégralAbedi, Syeda Husna Bano. « Mechanisms of migration of vascular smooth muscle and endothelial cells : role of the focal adhesion kinase pathway ». Thesis, King's College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286793.
Texte intégralRioja, Ana Ysabel. « Cell Adhesion and Migration on NDGA Cross-Linked Fibrillar Collagen Matrices for Tendon Tissue Engineering ». Scholar Commons, 2012. http://scholarcommons.usf.edu/etd/4214.
Texte intégralNg, Lui, et 吳磊. « Actopaxin : a novel regulator of cell migration and invasion in human hepatocellular carcinoma ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B47752610.
Texte intégralpublished_or_final_version
Surgery
Doctoral
Doctor of Philosophy
Xiong, Siyuan. « The role of Activin B and TGFb1 in regulating endometrial cancer cell adhesion and migration ». Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/58581.
Texte intégralMedicine, Faculty of
Obstetrics and Gynaecology, Department of
Graduate
Dawson, Cassandra. « Investigating the role of protein tyrosine phosphatase alpha in focal adhesion dynamics and cell migration ». Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/62137.
Texte intégralMedicine, Faculty of
Experimental Medicine, Division of
Medicine, Department of
Graduate
Smith, Liisa Sundberg Taylor Joan M. « A role for focal adhesion kinase in vascular smooth muscle cell proliferation, migration and differentiation ». Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,1258.
Texte intégralTitle from electronic title page (viewed Mar. 26, 2008). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Pathology and Laboratory Medicine." Discipline: Pathology and Laboratory Medicine; Department/School: Medicine.
Bola, Becky Melanie. « The role of Kinesin-1 and Rab Proteins in cell migration and focal adhesion dynamics ». Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.491863.
Texte intégralInfante, Elvira. « Role of Rap and Rho GTPases in T-acute lymphoblastic leukaemia cell adhesion and migration ». Thesis, King's College London (University of London), 2013. https://kclpure.kcl.ac.uk/portal/en/theses/role-of-rap-and-rho-gtpases-in-tacute-lymphoblastic-leukaemia-cell-adhesion-and-migration(e1c1aff6-96a7-4dc6-9ee5-355bb41b01ee).html.
Texte intégralCarvalho, Adriana Andrade. « Estudo do potencial antimetastÃtico da biflorina ». Universidade Federal do CearÃ, 2011. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6949.
Texte intégralA presenÃa de metÃstase permanece como a principal causa de morte pelo cÃncer. Diante da ausÃncia de terapia farmacolÃgica para o tratamento de tumores secundÃrios, a pesquisa de novas drogas com potencial antimetastÃtico à de suma importÃncia para o desenvolvimento de novos fÃrmacos anticÃncer. Neste quadro, decidimos avaliar o potencial antimetastÃtico da biflorina, uma o-naftoquinona isolada das raÃzes da Capraria biflora. Em ensaio de proliferaÃÃo celular por Alamar blue, observamos que esta quinona possui atividade citotÃxica contra melanoma humano (MDAMB-435) a partir da concentraÃÃo 5 ÂM em 24h de exposiÃÃo. PorÃm, nessa mesma dose, nÃo houve efeito citotÃxico em 12h de exposiÃÃo. Ensaios de azul de tripan e cristal violeta mostraram que nas concentraÃÃes de 1,0; 2,5 e 5,0 ÂM durante 12h de exposiÃÃo a biflorina nÃo possui efeito citotÃxico. Utilizando as concentraÃÃes de 1,0; 2,5 e 5,0 ÂM (12h exposiÃÃo) foram realizados ensaio de migraÃÃo e invasÃo celular. Nestes ensaios observamos que a biflorina diminui a motilidade e a invasividade da cÃlula MDAMB-435. Em anÃlise morfolÃgica das cÃlulas, utilizando coloraÃÃo de May-Grunwald-Giemsa e coloraÃÃo de actina por faloidina, observamos que a biflorina altera a organizaÃÃo do citoesqueleto de actina, com a presenÃa de cÃlulas menores, retraÃdas e cÃlulas maiores com expansÃes filamentosas semelhantes à filopÃdios. Em ensaio de Western blot observou-se a diminuiÃÃo na expressÃo da molÃcula de adesÃo N-caderina e inibiÃÃo da via de sinalizaÃÃo PI3K/Akt. Estes resultados conferem à biflorina um potencial antimetastÃtico bastante promissor.
Metastasis remains the leading cause of death from cancer. Due to the absence of pharmacological therapy for the treatment of secondary tumors, the search for new drugs with antimetastatic potential is important to the development of new anticancer drugs. In this context we decided to evaluate the antimetastatic potential of biflorin, an o-naphthoquinone isolated from roots of Capraria biflora. In cell proliferation assay using Alamar blue, we found that this quinone has cytotoxic activity against human melanoma cells line (MDAMB-435) at 5 ÂM concentration during 24 hours of exposure. However, with this same dose, there was no cytotoxic effect within 12 hours of exposure. Trypan blue and crystal violet assay showed that biflorina has no cytotoxic effect at 1.0, 2.5 and 5.0 ÂM during 12 hours of exposure. Migration assay and cell invasion assay were performed using concentrations of 1.0, 2.5 and 5.0 ÂM (12h exposure). In these trials we found that biflorin decreases cell motility and invasiveness. In morphological analysis of cells stained using May-Grunwald-Giemsa and actin stain by phalloidin, we observed that biflorin alters the organization of the actin cytoskeleton, with the presence of smaller, retracted and larger cells. In Western blot assay we observed a decrease in the expression of the adhesion molecule N-cadherin and inhibition of PI3K/AKT signaling pathway. These results give biflorin as an agent with promising antimetastatic potential.
Imtiaz, Sarah [Verfasser], Philippe [Akademischer Betreuer] Philippe et Leif [Gutachter] Dehmelt. « Distinct focal adhesion protein modules control the adhesion site segregation and cell migration behavior / Sarah Imtiaz ; Gutachter : Leif Dehmelt ; Betreuer : Philippe Philippe ». Dortmund : Universitätsbibliothek Dortmund, 2017. http://d-nb.info/1175625469/34.
Texte intégralTimpson, Paul. « A study examining the role of Rho family GTPases in the intracellular targeting of Src kinase during cell polarisation and migration ». Thesis, University of Glasgow, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248188.
Texte intégralHuang, Zhiyao. « SUMOylation regulates focal adhesions in cancer cell migration ». Thesis, University of Reading, 2017. http://centaur.reading.ac.uk/73251/.
Texte intégralGhosh, Somadri. « A signalling function of phosphatidylinositol 3,4-bisphosphate in cell migration of breast cancer cells ». Doctoral thesis, Universite Libre de Bruxelles, 2018. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/268960.
Texte intégralDoctorat en Sciences biomédicales et pharmaceutiques (Médecine)
info:eu-repo/semantics/nonPublished
Banu, Nahida Arjumand. « The role of selective cyclooxygenase-2 inhibitor NS398 in the regulation of cell adhesion and cell migration in colorectal neoplasia ». Thesis, University of Bristol, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.404309.
Texte intégralBirner, Ulrieke. « Cell adhesion molecule mechanisms for neutrophil and monocyte migration to joints of rats with adjuvant arthritis ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0017/MQ49315.pdf.
Texte intégralStrugnell, Scott Stuart. « The role of tyrosine phosphorylated caveolin-1 in regulating focal adhesion dynamics and cancer cell migration ». Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/15218.
Texte intégralSalo, S. (Sirpa). « Laminin-5:function of the γ2 chain in epithelial cell adhesion and migration, and expression in epithelial cells and carcinomas ». Doctoral thesis, University of Oulu, 1999. http://urn.fi/urn:isbn:9514253426.
Texte intégralJensen, Rebecca Leah. « Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces : A Micro-Incubator in vitro Model ». Cleveland State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419.
Texte intégralComber, Kate. « Investigation into the molecular mechanisms governing Drosophila embryonic hemocyte migration in vivo ». Thesis, University of Bath, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606669.
Texte intégralMaccormac, Luci Penelope. « The effect of cytomegalovirus infection of endothelial cells on cell surface molecules involved in leukocyte adhesion, migration and immune recognition ». Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300625.
Texte intégralCichon, Monika Agnieszka. « Axl regulates adhesion, migration and stem-like properties of cancer cells ». Thesis, Queen Mary, University of London, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610930.
Texte intégralStroeken, Peter Johannes Marie. « Adhesion molecules in lymphoma cell migration and metastasis the role of the [beta]1 integrin cytoplasmic domain / ». [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2002. http://dare.uva.nl/document/61380.
Texte intégralCarvalho, Adriana Andrade. « Estudo da atividade antimetastÃtica da biflorina, uma o-naftoquinona isolada das raÃzes de Capraria biflora ». Universidade Federal do CearÃ, 2009. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3565.
Texte intégralA principal causa de mortalidade em pacientes com cÃncer està relacionada com a presenÃa de tumores secundÃrios pelo organismo. Devido a falta de tratamento das metÃstases, muitos esforÃos estÃo sendo lanÃados para o desenvolvimento de novas drogas com potencial antimetastÃtico. Em estudos realizados em nosso laboratÃrio observamos que a biflorina, uma o-naftoquinona isolada das raÃzes da Capraria biflora, aumentava a sobrevida dos animais transplantados com o melanoma B16 sem expressiva toxicidade. Diante desse dado resolvemos avaliar a atividade antimetastÃtica desta naftoquinona, em modelos experimentais in vivo e in vitro, utilizando as linhagens celulares B16-F10 (melanoma murino) e MDAMB-435 (melanoma humano). A induÃÃo da metÃstase experimental foi realizada pela inoculaÃÃo da linhagem celular B16-F10 via veia caudal de animais C57BL/6. Neste ensaio, a biflorina (25 e 50 mg/kg/dia) inibiu a formaÃÃo dos nÃdulos metastÃtico em 57 e 71 %, respectivamente. Entretanto, em anÃlise histopatolÃgica dos pulmÃes dos animais tratados, foi observada a presenÃa de hemÃcias e hemossiderina, o que indica a presenÃa de hemorragia recente e tardia. Com objetivo de avaliar como a biflorina tem seu efeito sobre a metÃstase, foram realizados alguns ensaios in vitro utilizando duas linhagens de melanoma metastÃtico: B16-F10 e MDAMB-435. Em ensaio de adesÃo celular, a biflorina foi capaz de inibir a adesÃo de ambas as cÃlulas sobre o colÃgeno tipo I, um dos constituintes da matriz extracelular. AlÃm disso, em ensaio de migraÃÃo celular, utilizando o mÃtodo Wound healing (cicatrizaÃÃo), a biflorina tambÃm foi capaz de inibir a motilidade destas cÃlulas. Vale ressaltar que nesses ensaios foram utilizadas concentraÃÃes nÃo-citotÃxicas, o que exclue um efeito falso positivo. Por fim, em ensaio de zimograma com gelatina, foi observado que a biflorina nÃo alterava a liberaÃÃo das metaloproteinases -2 e -9 para o meio de crescimento, excluindo esse mecanismo de aÃÃo. Esses resultados sugerem que a biflorina apresenta um potencial antimetastÃtico bastante promissor atravÃs da sua aÃÃo sobre a adesÃo e migraÃÃo celular, eventos cruciais para que ocorra a formaÃÃo de metÃstase. Entretanto, futuros estudos sÃo necessÃrios para elucidar seu mecanismo de aÃÃo.
The main cause of mortality in cancer patients is related to the incidence of secondary tumors in the body. Owning up to the deficiency of therapeutic schemes direct to the treatment of metastasis, many efforts are being launched to develop drugs with anti-metastatic potential. In previous studies performed in our laboratory, biflorin, an o-naphthoquinone isolated from the roots of Capraria biflora, was found to increase the survival rates of B16-bearing mice without significant toxicity. In spite of these findings we decided to evaluate the anti-metastatic activity of biflorin using B16-F10 (murine melanoma) and MDAMB-435 (human melanoma) cells line. The experimental metastasis model was achieved by injecting B16-F10 cells in the tail vein of C57BL/6 mice. In this assay, biflorin (25 and 50 mg/kg/day) inhibited the formation of metastatic nodules in 57 and 71 %, respectively. Nevertheless, histopathological analyses of biflorin-treated lungs showed the presence of erythrocytes and hemosiderin, indicating the occurrence of recent and late hemorrhage. In order to evaluate how biflorin inhibits metastasis, we carried out in vitro tests using two cell lines of metastatic melanoma, B16-F10 and MDAMB-435. In the cell adhesion assay, biflorin inhibited adhesion of both cells lines on type I collagen, a substrate of the extracellular matrix. Moreover, biflorin was able to inhibit cell motility in the wound healing assay. The concentrations used in these assays did not show any cytotoxicity after 24 h of incubation, excluding a false-positive. Even so, in the zymogram assay we observed that biflorin did not alter the release of metallopeptidases -2 and -9 into growth medium, thus excluding this as the means by which biflorin exerts the anti-metastatic effect. These data suggest that biflorin has a promising anti-metastatic potential, as shown by its anti-adhesion and anti-migration properties on metastatic melanoma cell lines, however further studies are indispensable to elucidate its action mechanism.
Silveira, Bernardo Salim. « Análise da expressão e distribuição de E-caderina, Vinculina e cinase de adesão focal em biópsias de carcinoma espinocelular oral ». reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/147801.
Texte intégralSquamous cell carcinoma is a malignant neoplasm that accounts for approximately 94% of all occurrences present in mouth and one of its main characteristics is the cellular migration of its cells to form metastases. Cell adhesion is considered one of the defining events of cell migration. For a three-dimensional tissue structure, adhesions between cells and between cells and the extracellular matrix is of great importance. Cell adhesion junctions arise characteristically by interaction between adhesive receptors, signaling pathways and cytoskeletal elements. The protein E-cadherin is present in cells in the adhesion between epithelial tissue. The Focal Adhesion Kinase (FAK) protein is involved in most events related to cell adhesion stimulated by integrins. The vinculin is an adhesion protein that binds cytoskeletal protein through integrins activaion. Recent studies suggest that there are alterations in the expression and activity of adhesion proteins in malignant tumors. The aim of this study was to describe the pattern of expression and regulation of the activity of adhesion proteins in tumor samples of squamous cell carcinoma. Immunohistochemical reactions were performed to check the distribution pattern of the protein E-cadherin, vimentin and FAK-y397 in tumor samples of oral squamous cell carcinoma. There was a decrease in the expression of E-cadherin and vinculin in regions of cell-cell adhesion but, on the other hand, it was found to increase in cytoplasmic as well as unscheduled vinculin FAK-y397 in all tumor samples. Despite progress, it is necessary more observational studies that examine not only the degree of expression of these adhesion proteins, but also its level of regulation. From the results of this study it is suggested that the control of the expression level and activity of cell adhesion may be considered as potential targets for application adjuvant therapies that aim to reduce or prevent tumor progression and the development metastases.
Deem, Tracy L. « VCAM-1 Signaling in Endothelial Cells for Lymphocyte Migration ». University of Cincinnati / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1096565014.
Texte intégralSchroen, Daniel J. Cheung H. Tak. « Effect of entactin and other extracellular matrix molecules on the adhesion and migration of mouse thymocytes and a thymocyte cell line ». Normal, Ill. Illinois State University, 1994. http://wwwlib.umi.com/cr/ilstu/fullcit?p9507287.
Texte intégralTitle from title page screen, viewed March 21, 2006. Dissertation Committee: H. Tak Cheung (chair), Herman E. Brockman, Lynne A. Lucher, Philip D. Morse, Anthony J. Otsuka. Includes bibliographical references (leaves 101-111) and abstract. Also available in print.