Littérature scientifique sur le sujet « Carbohydrate Functionalized Nanomaterials »

Numerous abiotic stresses trigger the overproduction of reactive oxygen species (ROS) that are highly toxic and reactive. These ROS are known to cause damage to carbohydrates, DNA, lipids and proteins, and build the oxidative stress and results in the induction of various diseases. To resolve this issue, antioxidants molecules have gained significant attention to scavenge these free radicals and ROS. However, poor absorption ability, difficulty in crossing the cell membranes and degradation of these antioxidants during delivery are the few challenges associated with both natural and synthetic antioxidants that limit their bioavailability. Moreover, the use of nanoparticles as an antioxidant is overlooked, and is limited to a few nanomaterials. To address these issues, antioxidant functionalized nanoparticles derived from various biological origin have emerged as an important alternative, because of properties like biocompatibility, high stability and targeted delivery. Algae, bacteria, fungi, lichens and plants are known as the producers of diverse secondary metabolites and phenolic compounds with extraordinary antioxidant properties. Hence, these compounds could be used in amalgamation with biogenic derived nanoparticles (NPs) for better antioxidant potential. This review intends to increase our knowledge about the antioxidant functionalized nanoparticles and the mechanism by which antioxidants empower nanoparticles to combat oxidative stress.

Gastric cancer is one of the most common malignant tumors, and early diagnosis will be of great significance to improve the survival quality and overall treatment outcome evaluation of patients. Nanoelectrochemical immunosensor is an emerging biosensor combining nanotechnology, electrochemical analysis method and immunological technology, which has simple operation, fast analysis speed, high sensitivity, and good selectivity. This mini-review summarized immunoassay techniques, nanotechnology and electrochemical sensing for the early detection of gastric cancer. In particular, we focus on the tension of carbon nanomaterials in this field, including the functionalized preparation of materials, signal enhancement and the construction of novel sensing interfaces. Currently, various tumor markers are being developed, but the more recognized gastric cancer tumor markers are carcinoembryonic antigen (CEA), carbohydrate antigen (CA), CD44V9, miRNAs, and programmed death ligand 1. Among them, the electrochemical immunosensor allows the detection of CEA, CA, and miRNAs. The mini-review focused on the development of using carbon based materials, especially carbon nanotubes and graphene for immunosensor fabrication and gastric cancer markers detection.

Objectives: Platelet-, erythrocyte- and plasma-related products are vital for some patients. The main problems with these products are storage lesions, limitations in shelf life, and maintenance of function and quality. Dendrimers, a well-known group of polymeric nanoparticles, may help overcome these challenges due to their special properties. Innovation: This review article, for the first time, comprehensively discusses studies from 2010 to 2022 on the compatibility of positive, negative, neutral, and modified charge dendrimers with each blood product. Moreover, it provides information regarding dendrimers' applications for improving the quality and function of blood products. Results: A total of one hundred and twenty-six studies showed that dendrimers affect blood components depending on their load, size, molecular weight, functional group, concentration, and exposure time. Generally, cationic dendrimers with higher concentrations and molecular weight, and larger size showed little hemocompatibility, while anionic or neutral dendrimers with lower concentrations and molecular weight, and small size were more hemocompatible. Further, some modifications of cationic dendrimers were found to improve their compatibility. For erythrocytes, they included PEGylation and thiolation of dendrimers or functionalizing them with cyclic RGD, n-maleyl chitosan, zwitterionic chitosan, prednisolone, or carbohydrates. Additionally, dendrimers functionalized with arginine-birch, lysine-Cbz, polyethylene glycol, polyethylene glycol-cyclic RGD, thiol, TiO2, maltotriose, or streptokinase decreased the platelet toxicity of dendrimers. In the case of albumin products, the dendrimers modified with polyethylene glycol, glucose, and gold nanoparticles showed increased compatibility. Moreover, the PAMAM-dendrimer-antibody conjugates had no adverse effect on antibodies. Dendrimers have a wide range of applications, including virus detection kits, synthetic O2 carriers, bacterial nanofilters, drug carriers, anticoagulants, and enhanced blood products storage. Conclusion: It can be concluded that due to the outstanding properties of different types of dendrimers, particularly their manipulability, nanomaterials can be promising to enhance the quality of blood products. Thus, further research in this area is required.

This thesis focuses on the development of carbohydrate coupling chemistry on nanomaterials and their biological activity studies. It is divided into two parts: In part one, two carbohydrate immobilization approaches, based on perfluorophenyl azide (PFPA)-functionalized silica nanoparticles (SNPs), are presented, where the binding affinity of the glyconanoparticles was evaluated through carbohydrate-lectin interaction. In the first approach, PFPAfunctionalized SNPs were treated with propargylated glycosides and functionalized under copper-catalyzed azide-alkyne cycloaddition (CuAAC) conditions to give glyconanoparticles. For the second approach, a metal-free coupling chemistry based on perfluorophenyl azide-aldehyde-amine cycloaddition (AAAC) was developed for carbohydrate immobilization on PFPA-functionalized SNPs using glycosyl amine and phenylacetaldehyde. Subsequently, a quantitative fluorine nuclear magnetic resonance (19F qNMR) technique was developed to determine the carbohydrate density on the glyconanoparticles. The addition of an internal standard allowed the accurate determination of carbohydrate density, which was then used to calculate the apparent dissociation constant (Kd ) of the glyconanoparticles with lectin by a ligand competition assay. The developed approaches proved general and versatile, and the carbohydrate-presenting nanoplatforms showed high binding specificity in lectin binding. In part two, microwave irradiation was used to functionalize carbon nanomaterials with PFPA followed by carbohydrate conjugation. The microwave-assisted method proved efficient for a number of carbon nanomaterials including carbon nanotubes (CNTs), graphene and fullerene. The carbohydrates on the glyconanomaterials retained their binding patterns towards cognate lectins.

QC 20150907