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Kuusk, Sandra. « Control of Pistil Development in Arabidopsis thaliana by a Novel Class of Regulatory Genes ». Doctoral thesis, Uppsala : Universitetsbiblioteket : Univ.-bibl. [distributör], 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3366.
Texte intégralHoller, Silvia. « Droplet based synthetic biology : chemotaxis and interface with biology ». Doctoral thesis, Università degli studi di Trento, 2018. https://hdl.handle.net/11572/369014.
Texte intégralHoller, Silvia. « Droplet based synthetic biology : chemotaxis and interface with biology ». Doctoral thesis, University of Trento, 2018. http://eprints-phd.biblio.unitn.it/3465/1/tesi_dottorato_Holler_finale.pdf.
Texte intégralNilsen, Liv Sigrid. « Coastal heath vegetation in central Norway ; recent past, present state and future possibilities ». Doctoral thesis, Norwegian University of Science and Technology, Department of Biology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-250.
Texte intégralRohloff, Jens. « Cultivation of Herbs and Medicinal Plants in Norway - Essential Oil Production and Quality Control ». Doctoral thesis, Norwegian University of Science and Technology, Department of Biology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-415.
Texte intégralEssential oils (EO) are plant secondary metabolites that are known for their fragrance and food flavour properties. They consist of a complex mixture of mono- and sesquiterpenes, phenyl propanoids and oxygenated compounds. EOs can be present in different plant organs and materials, and their storage is related to specialised secretory structures. The yield of EOs from plant raw materials by distillation or pressing may on average vary from 0.1 – 1%, thus restricting the major EO production to the plant group of aromatic plants. Due to their function as signalling compounds between different types of organisms and diverse biological systems, their general antimicrobial and antioxidative effects and medicinal activity, EOs offer a promising potential for future applications within the fields of agriculture, medicine, pharmaceutical industry and biotechnology.
Changed consumer demands and raised interest in natural product compounds, especially essential oils, have formed the basis for initiating the research project “Norwegian Herb Production (Norsk Urteproduksjon NUP)” to encourage the cultivation, processing, marketing and distribution of aromatic and medicinal plants. The production, composition and quality characteristics of EOs (yield and terpene composition) from chamomile, lemon balm, oregano, peppermint, sachalinmint, thyme and yarrow have been investigated in the project period between 1994-1998.
Much focus has been put on the application of solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) for the analysis of EO volatiles from various aromatic and medicinal plants. SPME is a fast, solvent-free and non- destructive sample preparation technique where the analytes are extracted from fluid or solid matrices by headspace (HS) or direct immersion sampling (DI). Apart from EO isolation by common distillation, the applicability and sensitivity of the SPME fibre has made it feasible to carry out qualitative and semi-quantitative HS analyses of aromatic plants with regard to changes of EO metabolism during ontogenesis and plant development.
Based on NUP-results from field trials in the period between 1995-1996, the mint species peppermint (Mentha × piperita L.) and sachalinmint (Mentha sachalinensis (Briq.) Kudô) have been studied in detail (Papers B, D and E). Comparative analyses by applying distillation sampling and SPME have been carried out in order to study the advantages and disadvantages of both techniques (Papers B and E). It could be shown, that SPME offers a fast and reliable method for detecting quality-impact compounds from the p-menthane group (menthol, menthone, neomenthol, isomenthone and menthyl acetate). A distinct increase in the menthol/menthone ratio in the basipetal direction could be detected for peppermint and sachalinmint by applying SPME, thus revealing within-plant quality differences according to pharmacopeial requirements. Taking the increase of EO production from the vegetative to the generative growth stage into account, the harvest of mint plants in bloom will result in better EO yield and quality with regard to higher amounts of menthol.
When applying HS-SPME on complex EO volatile matrices such as known for yarrow (Achillea millefolium L.; Paper C), one might deal with fibre-partitioning effects of the different mono- and sesquiterpenes due to their physical and chemical properties. Despite these disadvantages, HS-SPME appears to be a sensitive extraction method for the screening of EO volatiles from complex sample matrices. Comparative analyses of volatiles from rose root rhizomes (Rhodiola rosea L.) have been carried out in order to characterize the rose-like odour compounds (Paper F). A total of 75 and 59 compounds have been identified by distillation sampling and HS-SPME, respectively, thus underscoring the excellent extraction properties and applicability of the SPME fibre.
Paper A gives a brief overview of EO biosynthesis and chemical structures, plant sources and methods of EO production. Before leading over to the main topic of HS-SPME applications by referring to numerous examples from the research work at The Plant Biocenter in the past 5 years, an introduction of solid-phase microextraction with regard to devices, procedures and extraction parameters is given.
The advantages and disadvantages of distillation vs. SPME are outlined on the background of comparative analyses of peppermint, chamomile, basil and dill. Furthermore, the utilization of HS-SPME for quantitative studies with regard to extraction time and analyte concentration is being highlighted. Examples for the screening of chemotypes (hops −Humulus lupulus L.) and cultivars (dill – Anethum graveolens L.) and ontogenetic studies are given (Mentha species; arnica −Arnica montana L.). Finally, the applicability of HS-SPME for the quality assessment of processed herbs (sweet basil −Ocimum basilicum L.) and phytomedicinal preparations (red coneflower – Echinacea purpurea L.) is being discussed.
The advantages of HS-SPME over classical distillation and headspace applications are impressive due to drastically reduced analysis time and will introduce new frontiers in plant volatile research with regard to secondary metabolism, plant-insect interactions and in vivo studies. The user-friendliness of operating SPME will initiate the development of future applications and equipment for the monitoring of volatiles for plant biological and environmental studies, extraction automation, on-site sampling and on-fibre storage of analytes.
Paper VI reprinted with kind permission of Elsevier, Sciencedirect, www.sciencedirect.com
Espmark, Åsa Maria. « Behavioural effects on environmental pollution in threespine stickleback Gasterosteus aculeatus L ». Doctoral thesis, Norwegian University of Science and Technology, Department of Biology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-423.
Texte intégralThe aims of this study were to investigate the effects of known environmental contaminants on defined behavioural variables in fish, and to discuss properties of these behavioural traits that make them useful as potential indicators of pollution.
In studying the effects of pollution, the resulting biochemical and physiological alterations are more commonly measured. However, effects of pollution can manifest itself at all levels of biological organisation, including behaviour. In this respect, behaviour can be considered a valid biomarker of pollution in that it is expected to be both susceptible to pollution and of high ecological significance, as it influences the fitness of the affected individuals.
This thesis is based on four individual studies, in which the threespine stickleback Gasterosteus aculeatus was used as a model species. Results from these studies show that antipredator behaviour, feeding behaviour, shoaling behaviour, bottom-dwelling behaviour and reproductive behaviour are all sensitive to exposure to sublethal concentrations of defined environmentally relevant chemicals.
The results showed that antipredator behaviour and fright response in threespine stickleback were impaired following exposure to sublethal concentrations of bis(tributyltin)oxide (TBTO). However, for some of the tested antipredator variables the effects were reversed after the ending of exposure. Further, it was shown that feeding motivation in fish exposed to butyl benzyl phthalate (BBP) and/or 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (DDE) was increased in that exposed fish initiated feeding more often than the controls. Exposure to BBP also caused sticklebacks to aggregate into tight shoals and to spend more time at the bottom of the aquarium compared to the control fish.
The reported significant differences between the controls and BBPexposed fish with respect to feeding and shoaling behaviour were shown even though the levels of BBP were below the analytical detection limit. Different suggested explanations, for example, too high detection limit, or degradation to its BBP metabolites are given to this result.
17β-Oestradiol (E2) exposed male sticklebacks started nest building later than non-exposed males, but there were no differences between exposed and control males with respect to the number of males that built nests. Further, the exposed males spent less time displaying paternal care compared to the control males, although there were no differences between the two groups in the number of performed courtship displays. Because of the significant effect upon some but not all reproductive behavioural traits, it was suggested that the different variables might vary in sensitivity, implying that a variety of variables should be studied in order to obtain a more reliable evaluation of the effects of pollution.
Chemicals can cause deleterious effects at one or more levels of biological organisation, from biochemical, physiological, individual, population and through to the ecosystem levels. In contrast to the established hypothesis that a pollutant affects the different biological levels in an escalating timedependent pattern, starting at the biochemical level, it is here suggested that biomarkers at the biochemical, physiological and behavioural levels often will respond early and simultaneously in the same individual.
Whereas some biochemical responses are specifically related to one class of exposure agents and thus may act as specific indicators of pollution, most behavioural traits may be altered in response to a variety of chemicals. One exception may be alterations in reproductive behaviour caused by endocrine disrupting chemicals, due to effects of the chemicals on hormones that result in immediate reproductive behavioural effects. In spite of the specific action of some biochemical biomarkers, they are often considered to be of little ecological relevance since many of them are not related to individual fitness.
In this thesis, it is argued that behavioural variables can be employed as useful and reliable biomarkers of environmental contamination. It is also important to focus on behaviour to map and quantify the resposes. However, to reliably evaluate the effects of pollution, behavioural variables should be used in association with biochemical and physiological traits. Moreover, optimal combination of results from laboratory and field experiments would enhance the ecological relevance of the study.
All papers reprinted with kind permission of Elsevier, sciencedirect.com
Solberg, Bård Øyvind. « Effects of climatic change on the growth of dominating tree species along major environmental gradients ». Doctoral thesis, Norwegian University of Science and Technology, Department of Biology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-487.
Texte intégralThis thesis deals with effects of climate on tree growth of the dominating conifer species, Picea abies (L) Karst. (Norway spruce) and Pinus sylvestris L (Scots pine), in central Norway and Fennoscandia. Both species are sampled along major environmental gradients, i.e. altitude and oceanicity, and growth responses to climate, i.e. temperature and precipitation, are examined along these gradients. Additionally, time is considered as an environmental gradient and temporal responses are carefully deciphered. Special attention is given to large-scale climate oscillation and their effect on tree growth. In the individual papers the specific aims have been to:
1. identify climate variables (all seasons) with significant influence on radial tree growth of P. abies and P. sylvestris along major environmental gradients (Paper I-IV)
2. identify if and how the growth response to climate has changed through time along these gradients (Paper I-IV)
3. make interregional comparisons of P. sylvestris growth pattern across Fennoscandia from oceanic western Norway to continental eastern Finland (Paper III)
4. analyse to what degree large-scale circulation patterns of air masses are registered in regional tree growth of both P. abies and P. sylvestris (Paper I, III and IV)
5. discuss possible effects on radial tree growth of a predicted warmer climate (Paper I, III and IV)
Røstelien, Tonette. « Functional characterisation of olfactory receptor neurone types in heliothine moths : Identification of molecular receptive ranges by the use of single cell recordings linked to gas chromatography and mass spectrometry ». Doctoral thesis, Norwegian University of Science and Technology, Department of Biology, 2005. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-570.
Texte intégralWhen the study of this thesis was initiated, hardly any work had been carried out on how plant odour information was encoded by the olfactory RNs in heliothine moths. The method of gas chromatography linked to single cell recordings (GC-SCR) was employed and improved for identifying naturally occurring plant odorants that are detected by single RNs and can be considered as biologically relevant. Three species of the subfamily Heliothinae were included in this work, the two polyphagous H. virescens and H. armigera and the oligophagous H. assulta. The American H. virescens is geographically separated from the other two species. H. armigera and H. assulta are partly sympatric in Asia and Australia.
The aims of the thesis elucidated in Papers I-IV were as follows:
1. To identify plant produced volatiles detected by antennal RNs in the three species of the subfamily Heliothinae.
2. To elucidate whether the single RNs can be classified into distinct types according to their specificity.
3. To characterise the plant odour RN types by their molecular receptive ranges, sensitivity and specificity.
4. To compare the specificity of plant odour RN types across the three related species of Heliothinae, with the aim to reveal any differences in the peripheral olfactory system that may have evolved through evolution.
Karlberg, Olof. « Mitochondrial Evolution : Turning Bugs into Features ». Doctoral thesis, Uppsala University, Molecular Evolution, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4216.
Texte intégralThe bacterial origin of mitochondria from an ancient endosymbiosis is now widely accepted and the mitochondrial ancestor is generally believed to belong to the bacterial subdivision α-proteobacteria. The high fraction of mitochondrial proteins encoded in the nucleus has commonly been explained with a massive transfer of genes from the genome of the ancestral mitochondrion.
The aim of this work was to get a better understanding of the mitochondrial origin and evolution by comparative genomics and phylogenetic analyses on mitochondria and α-proteobacteria. To this end, we sequenced the genomes of the intracellular parasites Bartonella henselae and Bartonella quintana, the causative agents of cat-scratch disease and trench fever, and compared them with other α-proteobacteria as well as mitochondrial eukaryotes.
Our results suggest that the adaptation to an intracellular life-style is coupled to an increased rate of genome degradation and a reduced ability to accommodate environmental changes. Reconstruction of the α-proteobacterial ancestor and phylogenetic analyses of the mitochondrial proteome in yeast revealed that only a small fraction of the proteins used for mitochondrial functions could be traced to the α-proteobacteria. Furthermore, a substantial fraction of the mitochondrial proteins was of eukaryotic origin and while most of the genes of the α-proteobacterial ancestor have been lost, many of those that have been transferred to the nuclear genome seem to encode non-mitochondrial proteins.
Klasson, Lisa. « Genome Evolution in Maternally Inherited Insect Endosymbionts ». Doctoral thesis, Uppsala University, Department of Evolution, Genomics and Systematics, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5885.
Texte intégralSymbiosis is a widely common phenomenon in nature and has undoubtedly contributed to the evolution of all organisms on earth. Symbiotic associations can be of varying character, such as parasitic or mutualistic, but all imply a close relationship. To study the evolution of genomes of insect endosymbionts, we have sequenced the genomes of the mutualist Buchnera aphidicola from the aphid Schizaphis graminum (Sg) and the reproductive manipulator Wolbachia pipientis strain wRi from Drosophila simulans that show strikingly different evolutionary patterns.
The comparison between the genome of B.aphidicola (Sg) and the genome of B.aphidicola from the aphid Acyrthosiphon pisum (Ap), that are believed to have diverged 50 million years ago, revealed a perfect gene order conservation and loss of only 14 genes in either of the lineages. In contrast, the rate of nucleotide turnover is very fast probably due to relaxed selection and loss of DNA repair genes. The genomic stasis observed in Buchnera was attributed to the loss of repeats and of the gene recA.
In striking contrast to the genomes of B.aphidicola, a vast amount of repeats were found in the genome sequence of W.pipientis strain wMel. The comparison between the genomes of W.pipientis strain wRi and W.pipientis strain wMel shows that a lot of rearrangements have occurred since their divergence. The massive amount of repeats might stem from relaxed selection pressure but possibly also from selection to create variability via recombination.
Comparisons between pairs of genomes from closely related bacteria showed that the stability of gene order and content is connected to an intracellular lifestyle and indicated that homologous recombination between repeats is an important mechanisms for causing intrachromosomal rearrangements. Our studies show that the lifestyle of a bacterium to a great extent shapes the evolution of their genetic material and future capabilities to adapt to new environments.
Costa, José Luis. « The tRNALeu (UAA) Intron of Cyanobacteria : Towards Understanding a Genetic Marker ». Doctoral thesis, Uppsala universitet, Fysiologisk botanik, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4537.
Texte intégralPrevodnik, Andreas. « The use of protein biomarkers in ecotoxicology : Studies of oxidative and genotoxic stress in the blue mussel (Mytilus edulis) ». Doctoral thesis, Stockholm : Department of Systems Ecology, Stockholm University, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-6755.
Texte intégralWredle, Ulla. « Autophagic programmed cell death in the suspensor and endosperm of Vicia faba : An ultrastructural study ». Doctoral thesis, Stockholm : Botaniska institutionen, Univ, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-123.
Texte intégralWinge, Per. « The evolution of small GTP binding proteins in cellular organisms. Studies of RAS GTPases in arabidopsis thaliana and the Ral GTPase from Drosophila melanogaster ». Doctoral thesis, Norwegian University of Science and Technology, Faculty of Natural Sciences and Technology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-169.
Texte intégralSmall GTP binding proteins function as molecular switches which cycles between GTP-bound ON and GDP-bound OFF states, and regulate a wide variety of cellular processes as biological timers. The first characterized member of the small GTPase family, the mutated oncogene p21 src, later known as Harvey-Ras, was identified in the early 1980s (Shih, T. Y. et al. 1980). In the following years small Ras-lik GTPases were found in several organisms and it was soon discovered that they took part in processes, such as signal transduction, gene expression, cytoskeleton reorganisation, microtubule organisation, and vesicular and nuclear transport. The first Rho (Ras homology) gene was cloned in 1985 from the sea slug Aplysia (Madaule, P. et al. 1985) and because of their homology to Ras it was first suspected that they could act as oncogenes. Later studies have shown that even though they participate in processes such as cell migration and motility they are not mutated in cancers.
The first indications that Rho was a signaling protein regulating the actin cytoskeleton, came from experiments where activated forms of human RhoA was microinjected into 3T3 cells (Paterson, H. F. et al. 1990). Another Rho-like GTPase Rac1 (named after Ras-related C3 botulinum toxin substrate) was later shown to regulate actin cytoskeletal dynamics as well, suggesting that Rho-family members cooperate in controlling these processes (Ridley, A. J. et al. 1992). The Rac GTPase was also implicated in regulating the phagocytic NADPH oxidase, which produce superoxide for killing phagocytized microorganisms (Abo, A. et al. 1991). Thus, it soon became clear that Rac/Rho and the related GTPase Cdc42 (cell division cycle 42) had central functions in many important cellular processes.
There are at least three types of regulators for Rho-like proteins. The GDP/GTP exchange factors (GEFs) which stimulates conversion from the GDPbound form to the GTP-bound form. GDP dissociation inhibitors (GDIs) decrease the nucleotide dissociation from the GTPase and retrieve them from membranes to the cytosol. GTPase activating proteins (GAPs) stimulates the intrinsic GTPase activity and GTP hydrolysis. In addition there are probably regulators that dissociate GDI from the GTPase leaving it open for activation by the RhoGEFs.
Ras and Rho-family proteins participate in a coordinated regulation of cellular processes such as cell motility, cell growth and division. The Ral GTPase is closely related to Ras and recent studies have shown that this GTPase is involved in crosstalk between both Ras and Rho proteins (Feig, L. A. et al. 1996; Oshiro, T. et al. 2002). Ral proteins are not found in plants and they appear to be restricted to animalia and probably yeast. During a screen for small GTPases in Drosophila melanogaster I discovered in 1993 several new members of the Ras-family, such as Drosophila Ral (DRal), Ric1 and Rap2. The functions of Ral GTPases in Drosophila have until recently been poorly known, but in paper 2 we present some of the new findings.
Rho-like GTPases have been identified in several eukaryotic organisms such as, yeast (Bender, A. et al. 1989), Dictyostelium discoideum (Bush, J. et al. 1993), plants (Yang, Z. et al. 1993), Entamoeba histolytica (Lohia, A. et al. 1993) and Trypanosoma cruzi (Nepomuceno-Silva, J. L. et al. 2001). In our first publication, (Winge, P. et al. 1997), we describe the cloning of cDNAs from RAC-like GTPases in Arabidopsis thaliana and show mRNA expressions pattern for five of the genes. The five genes analyzed were expressed in most plant tissues with the exception of AtRAC2 (named Arac2 in the paper), which has an expression restricted to vascular tissues. We also discuss the evolution and development of RAC genes in plants. The third publication, (Winge, P. et al. 2000), describe the genetic structure and the genomic sequence of 11 RAC genes from Arabidopsis thaliana. As most genomic sequences of the AtRACs we analyzed came from the Landsberg erecta ecotype and the Arabidopsis thaliana genome was sequenced from the Columbia ecotype, it was possible to compare the sequences and identify new polymorphisms. The genomic location of the AtRAC genes plus the revelation of large genomic duplications provided additional information regarding the evolution of the gene family in plants. A summary and discussion of these new findings are presented together with a general study of small Ras-like GTPases and their evolution in cellular organisms. This study suggests that the small GTPases in eukaryots evolved from two bacterial ancestors, a Rab-like and a MglA/Arp-like (Arf-like) protein. The MglA proteins (after the mgl locus in Myxococcus xanthus) are required for gliding motility, which is a type of movement that take place without help of flagella.
The second publication describes experiments done with the Drosophila melanogaster DRal gene and its effects on cell shape and development. Ectopic expression of dominant negative forms of DRal reveals developmental defects in eye facets and hairs, while constitutive activated forms affects dorsal closure, leaving embryos with an open dorsal phenotype. Results presented in this publication suggest that DRal act through the Jun N-terminal kinase (JNK) pathway to regulate dorsal closure, but recent findings may point to additional explanations as well. The results also indicate a close association between processes regulated by Rac/Rho and Ral proteins in Drosophila.
Mossman, Sally Patricia. « Investigations into the biology and molecular biology of alphaherpesvirus saimiri ». Thesis, University of Liverpool, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.291968.
Texte intégralCamacho, Diogo Mayo. « In silico cell biology and biochemistry : a systems biology approach ». Diss., Virginia Tech, 2007. http://hdl.handle.net/10919/27960.
Texte intégralPh. D.
Stranden, Marit. « Olfactory receptor neurones specified for the same odorants in three related heliothine species (Helicoverpa armigera, Helicoverpa assulta and Heliothis virescens). : Electrophysiology linked to gas chromatography and mass spectrometry ». Doctoral thesis, Norwegian University of Science and Technology, Department of Biology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-520.
Texte intégralHvordan lærer og husker vi dufter? Hvordan skiller vi dem fra hverandre?
Det finnes liten kunnskap om hvilke duftsubstanser ulike lukteceller er utviklet for. I dag er gener for luktereseptorer identifisert i mange organismer, men kunnskapen om relevante duftstimuli mangler. Denne kunnskapen kan kun komme fra fysiologiske studier. I laboratoriet ble det brukt beslektede skadeinsekter som modeller for å studere innkoding av luktinformasjon i sanseceller og generelle prinsipper for luktesansens mekanismer.
Dette ble utført ved bruk av elektrofysiologiske målinger av nerveimpulser fra enkelte celler på insektantennene, med direkte kopling til kjemiske analyser av substansene avgitt av plantene. Resultatene har gitt ny kunnskap om hvilke plantesubstanser disse insektene detekterer i naturen. I tillegg har resultatene vist at luktecellene kan klassifiseres i distinkte typer, og samme type finnes hos de tre beslektede artene. Hver type er spesialisert for en enkelt substans, men de svarer også på høye konsentrasjoner av noen få andre substanser med lignende molekylstrukturer. Det er ingen overlapping av substanser mellom de ulike celletypene, noe som viser hvordan luktinformasjonen formidles via spesifikke nerveceller til hjernen hos disse insektene. Atferdsreaksjoner på en spesielt interessant substans viste at substansen økte tiltrekningen av gravide hunninsekter og økte antallet egg lagt på planter med substansen.
Å kjenne de biologiske luktesubstansene er viktig for å studere luktesansen generelt. Informasjon om hvilke plantedufter de studerte insektene lukter, kan brukes for å bekjempe skadene de påfører avlinger over hele verden.
Bendiksen, Eldar Åsgard. « Environmental effects on lipid nutrition of farmed Atlantic salmon (Salmo salar L.) parr and smolts ». Doctoral thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for biologi, 2003. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-511.
Texte intégralMålet med dette studiet har vært å undersøke vanntemperaturens og saltholdighetens innvirkning på lipidernæringen hos parr og smolt av oppdrettet atlantisk laks (Salmo salar L.). Lakseparr ble holdt ved lav vanntemperatur (2ºC) i seks måneder mens de ble fôret med en av fire fôrtyper med ulike fettkilde (dvs. marin fiskeolje eller vegetabilsk olje) og ulik konsentrasjon (lav, 21% og høy, 34%). Responsene ved den lave temperaturen ble sammenlignet med responsene en fikk hos fisk holdt ved 8ºC i et full-faktorielt forsøksdesign. Fôrinntak og vekst ble opprettholdt ved 2ºC, men var lavere enn ved 8ºC. Over tid ble vekst og fôrutnyttelse forbedret, noe som indikerer en langtids akklimeringsrespons hos fisken ved den lave temperaturen. Totalt sett var utnyttelsen av fôret bedre ved den laveste temperaturen. En kunne observere en gradvis reduksjon i veksthastighet og fôrutnyttelse ved den høyeste temperaturen ettersom fisken ble større. Fisken kompenserte for lavere energitetthet i fôret ved å øke fôrinntaket. Ved den høyeste temperaturen var veksten bedre hos fisk fôret med lav-fett-fôrene. Det var også en tendens til forbedret tilvekst når vegetabilsk olje ble brukt. Det var ingen tegn til at vegetabilsk olje var dårligere enn marin fiskeolje til å fremme vekst hos lakseparr i ferskvann. Fisken som ble fôret med høy-fett-fôr ble fetere enn den som fikk lav-fett-fôr. Det indikerer lipostatisk regulering av fôrinntak. Fett- og proteinfordøyeligheten var høy både ved 2ºC og 8ºC, selv om både fett- og proteinfordøyeligheten var lavest ved 2ºC. Ved den laveste temperaturen, ga økt fettinnhold en forbedret fettfordøyelighet, og bruk av vegetabilsk olje i fôret ga bedre proteinfordøyelighet. Proteinretensjonen var høyere ved den høyeste temperaturen uavhengig av fôrtype, noe som indikerer at proteinet ble både lett fordøyd og omdannet til nytt vev. Effektene av fôrtype på akklimeringen til lav temperatur ble bestemt fra deponeringen av fettsyrer fra fôret i ulike vev og fra budsjetter for n-3 og n-6 essensielle fettsyrer (EFS). Fettsyresammensetningen i polare (membran) lipider og upolare (lagrings) lipider i muskel, innvoller og ’rest’ ble tydelig påvirket av oljene i fôret, og de upolare lipidene ble mer påvirket enn de polare lipidene. Retensjonen av n-6 EFS var lavere enn for n-3 EFS, og var uavhengig av temperatur. Retensjonen av n-3 EFS var høyere ved 2ºC, spesielt hos fisk som fikk et fiskeoljebasert fôr. Dette kan reflektere betydningen av n-3 HUFA fettsyrer i akklimeringen til lav temperatur. Imidlertid var de polare lipider hos fisk som ble gitt fôr med vegetabils olje, mer umettet (UFA:SFA forhold) enn hos fisk gitt fôr med marine fiskeoljer. Dette kan bety at vegetabilske oljer produserte fisk som var bedre i stand til å tåle eksponering til lav temperatur, samtidig som membranlipidene var mindre utsatt for oksidering som følge av et lavere innhold av n-3 HUFA fettsyrer (hovedsaklig EPA og DHA). Etter seks måneder i ferskvann ble fisken smoltifisert, etterfulgt av en 42-dagers periode i sjøvann. Fôrhistorie i ferskvannsfasen påvirket påvekst hos smolt. En positiv effekt av vegetabilsk olje ble funnet, men denne effekten ble bare funnet i grupper som hadde et skifte til et høy-fett-fiskeoljefôr ved overføring til sjøvann. Det var derfor tydelig at vegetabilsk olje ikke hadde negative konsekvenser for akklimering til lav temperatur eller for smoltifiseringen hos unglaks, og påfølgende tilvekst i sjøvann var bedre når vegetabilske oljer hadde blitt brukt. Dette indikerer at fettsyre (fett) behovet til atlantisk laks er forskjellig mellom ferskvann og sjøvann, og at forskjellene er knyttet til smoltifiseringen. Det kan derfor spekuleres i om saltholdigheten i miljøet er viktigere enn temperaturen i å bestemme fettsyrebehovet hos atlantisk laks.
Papers II and V reprinted with kind permission of Elsevier, sciencedirect.com.
Hedbom, Jenny. « Vattnets funktion och utformning i en modern trädgård ». Thesis, University of Gävle, Department of Mathematics, Natural and Computer Sciences, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-3422.
Texte intégralWood, Luke, et lukewood@ihug co nz. « Hot rod biology ». RMIT University. Applied Communication, 2006. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080111.102025.
Texte intégralIstrail, Sorin. « Computational molecular biology / ». Amsterdam [u.a.] : Elsevier, 2003. http://www.loc.gov/catdir/toc/fy037/2003051360.html.
Texte intégralCyr, Desjardins Eric. « Historicity in biology ». Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/14142.
Texte intégralOzato, Kenjiro. « Present and future of medaka biology : molecular biology to field surveys ». Laboratory of Freshwater Fish Stocks, Nagoya University, 1992. http://hdl.handle.net/2237/13774.
Texte intégralLau, Katherine Aik Hee. « Biology and Molecular Biology of New HIV-1 Recombinants from Malaysia ». Thesis, The University of Sydney, 2009. http://hdl.handle.net/2123/4129.
Texte intégralLau, Katherine Aik Hee. « Biology and Molecular Biology of New HIV-1 Recombinants from Malaysia ». University of Sydney, 2009. http://hdl.handle.net/2123/4129.
Texte intégralHIV-1 is the cause of the majority of global HIV infections. Not only being more virulent, and relatively easily transmitted than HIV-2, HIV-1 is also more extensively studied. HIV-1 is known for its highly recombinogenic nature, together with an extreme genetic variety, both attributable to an error-prone reverse transcriptase which gives rise to heterozygous virion. Sequence diversity of HIV-1 has resulted in identification of 9 subtypes of HIV-1 M group, as well as 43 circulating and a number of other unique recombinant forms of HIV-1. The extensive heterogeneity of HIV-1 has become the main consideration in vaccine development, mainly due to the inherent variability of HIV-1 and the frequent generation of new recombinant forms, which subsequently makes the effort to control the HIV-1 pandemic more challenging. The inter-subtype recombination event is a common phenomenon observed in Malaysia whereby there is a co-circulation of multiple HIV-1 subtypes; CRF01_AE and subtype B. Therefore, it becomes crucial to widen the knowledge of currently emerging CRF01_AE/B inter-subtype recombinants, in order to assist the future regional vaccine design and also to prevent wider spread of these strains. Concurrently, with a better understanding on the characteristics of HIV-1 CRF01_AE/B recombinant forms, further diversification of these strains can possibly be thwarted. The objectives of this study included, firstly to study the molecular epidemiology pattern of different HIV-1 strains, as well as to observe their frequency and distribution. Our second aim was to identify possible derivative from CRF33_01B, and also other new CRF01_AE/B inter-subtype recombinant forms in Malaysia. Thirdly, we aimed to identify possible biological advantages of the CRF33_01B isolates over its parental strains; CRF01_AE and subtype B. Currently, the HIV-1 epidemic in Malaysia is in a concentrated phase with evidence of predominance of both CRF01_AE and subtype B found among heterosexuals and injecting drug users, respectively. There is urgent necessity to apply a more detailed and continuous molecular characterization and epidemiological monitoring of these recombinant forms in Malaysia. We obtained plasma samples from 115 HIV-1-infected patients who attended HIV clinic at the University Malaya Medical Centre in Kuala Lumpur, Malaysia. The HIV-1 PR-RT, gp120-env and gp41-env genes were amplified and sequenced from 50 samples, while the remaining 65 samples were successfully studied at either one or two HIV-1 specific genomic regions. Cloning, phylogenetic analyses, together with bootscanning methods were employed to assign subtypes and to identify inter-subtype recombination based on all three genomic regions. From the plasma-derived sequences of 50 patients, 46% were found to harbour CRF01_AE, 10% and 6% had subtype B and B’, and a total of 18% of the patients were infected with CRF33_01B, while the remaining 18% of patients was found to have unique recombinant forms. As for the other 65 patients, majority of them harboured CRF01_AE and subtype B. This study shows that co-circulation of multiple HIV-1 subtypes and their recombinant strains are frequent in the Malaysian population, while capable of spreading to different HIV-1 risk groups. Possible recombination hotspots in CRF01_AE/B recombinants are suggested to be within the HIV-1 PR-RT gene region. Further, this study highlights the need to characterize and monitor the molecular epidemiology of these recombinant forms. The ideal environment for the inter-subtype recombination event to take place is created by the co-circulation and dual infections of both CRF01_AE and subtype B. With more HIV-1 CRF01_AE/B recombinant forms emerging and shaping the nature of HIV epidemic in Malaysia, certainly it will complicate the timely diagnosis of these molecularly altered HIV-1 forms. The recent identification of the novel CRF33_01B suggests the emergence of other new CRF01_AE/B inter-subtype recombinant forms in Malaysia, as preliminarily demonstrated in some HIV-1 patients identified in the first part of this study. The peripheral blood mononuclear cells (PBMCs) of these HIV-1 patients were co-cultured with those of healthy donors, which we then isolated the proviral genomic DNA. The nested long-range PCR was performed to obtain seven overlapping viral genome fragments that made up the whole viral genome. The detailed phylogenetic, as well as bootscan analyses confirmed the mosaic compositions and recombinant structures of the newly emerging CRF01_AE/B recombinant forms derived from CRF01_AE and subtype B. One of them in particular; HIV-1 isolate 06MYKLD46 is structurally similar to CRF33_01B, except for an extra subtype B fragment within the env region. It also has close phylogenetic relationship and similar breakpoints with CRF33_01B, mainly at the PR-RT region. Furthermore, the other three distinct HIV-1 recombinants; isolates 07MYKLD47, 07MYKLD48 and 07MYKLD49 also display near full-length genomes composed of the backbone of CRF01_AE, with insertions of subtype B fragments at different gene regions. These results indicate the high possibility of second generation of minor recombinant forms derived from CRF33_01B, as well as the continuous evolution and rapid dispersal of CRF01_AE/B recombinants in Malaysia. The high prevalence of newly emerging CRF33_01B (CRF01_AE/B inter-subtype recombinant) may cause a possible epidemiologic shift, attributable to its altered virologic characteristics and possible transmission advantages compared to its parental strains. Two major determinants; the viral factor and host factor have influenced the progress of a productive HIV-1 infection upon virus entry into the host cells. We have assessed the two main viral factors; the in vitro viral replication capacity and the viral fitness of the circulating HIV-1 strains in Malaysia. We have determined that CRF33_01B primary isolate (07MYKLVik) replicates better in activated whole PBMCs and CD4+ T-lymphocytes and is ‘fitter’ than one of its parental strain; CRF01_AE (07MYKLNBL) but not subtype B (07MYKLAfik). Subtype B has more advanced ability to produce a progressive infection in all cell types, including MDMs, and has a comparable viral fitness to that of CRF33_01B. We also investigated the role of host factors in a productive HIV-1 infection, by determining the viral effect on the host cell morphological features. We found that CRF33_01B (07MYKLVik) culture displayed more large syncytia (multinucleated giant cells) with multiple nuclei compared to subtype B (07MYKLAfik) culture, while no snycytia was observed in CRF01_AE (07MYKLNBL) culture. Generally, the cells within CRF33_01B and subtype B cultures appeared to be morphologically distinct from CRF01_AE cultures. This may indicate a more productive HIV-1 infection of CRF33_01B and subtype B, similar to our finding from the in vitro viral replicative capacity and viral fitness assays of these HIV-1 strains. We also studied the effect of different HIV-1 strain infections on host differential gene expression profiles, by using the PCR Array, which detects a total of 84 genes known to be involved in the host response to HIV-1 infection. It was observed that the in vitro infection with CRF33_01B isolates resulted in a more damaging effect on host cells and caused more apoptotic death within the infected cultures, compared to the isolates of its parental subtypes. Moreover, subtype B isolates resulted in a poorer cell response upon viral infection, compared to CRF01_AE/B isolate. Concurrently, it also gave less productive spread of viral infection within the infected cultures, in comparison to CRF01_AE/B isolate. We speculate that if the same scenario is reflected in vivo, CRF01_AE/B inter-subtype recombinant including CRF33_01B would have a better survival rate within the host upon their infection, in comparison to their parental strains. This again strengthens our presumption that CRF33_01B has potential ability to disseminate widely in the Malaysian population and gives a progressive change of the current molecular epidemiological trend by gradually replacing the current predominance of CRF01_AE in the country.
Wahlström, Katrin. « Sediment Requirements for Freshwater Pearl Mussel (Margaritifera margaritifera) Recruitment : Sedimentkraven för Rekrytering hos Flodpärlmussla (Margaritifera margaritifera) ». Thesis, Karlstad University, Division for Environmental Sciences, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-571.
Texte intégralAbstract
The sediment requirements for freshwater pearl mussel (Margaritifera margaritifera) recruitment, in 18 rivers in the counties of Västra Götaland, Örebro, Värmland and Västmanland in Sweden, were investigated. The top 4 cm of sediment in the rivers was analysed in terms of size, distribution and organic compound within the fine sediment. The aims of the study were to determine whether there is a relation between sediment particle size compound and freshwater pearl mussel recruitment as well as between organic compound in fine sediment and recruitment of mussels. The study shows that there is a significant difference in the amount of organic silt between non-recruitment and recruitment sites with a higher percentage of organic silt in recruitment sites. There is also a legible difference between the amounts of silt per sample between non-recruitment sites and recruitment sites where there was significantly more silt in sediment samples of non-recruitment sites. With the exception of fine sediment, no significant difference was found between non-recruitment and recruitment sites regarding size class distribution.
Sammanfattning
Sedimentkraven för rekrytering hos flodpärlmussla (Margaritifera maragaritifera) i 18 svenska vattendrag belägna i Västra Götalands, Örebro, Värmlands och Västmanlands län undersöktes. Vattendragens översta 4 cm sediment analyserades gällande storlek, fördelning och organisk sammansättning i finsedimentet. Målen med studien var att fastställa huruvida det finns förhållanden mellan sedimentets partikelstorleksammansättning och rekrytering av flodpärlmussla samt mellan finsedimentets organiska sammansättning och rekrytering av musslor. Studien visar en signifikant skillnad i organiskt finsediment mellan icke-rekryteringsplatser och platser med rekrytering där rekryteringsplatser hade högre procentuell andel organiskt finsediment. En signifikant skillnad påvisades också mellan andel finsediment per sedimentprov där sedimentprov från icke-rekryteringsplatser innehöll en högre procentuell andel finsediment. Förutom gällande finsediment påvisades ingen signifikant skillnad i sedimentets storleksdistribution mellan rekryterings- och icke-rekryteringsplatser.
Peiró, Douglas Fernando. « Ecologia do caranguejo Austinixa aidae (Righi, 1967) (Crustacea : Brachyura : Pinnotheridae) de uma praia arenosa do litoral Norte do Estado de São Paulo ». Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/59/59139/tde-17042008-100416/.
Texte intégralThe biological and ecological aspects of Austinixa aidae, a symbiotic crab inhabiting burrows of the Thalassinidea ghost shrimp Callichirus major, from Perequê-Açu beach, Ubatuba, North shore of State of São Paulo, Brazil, were studied. The samples were conducted bimonthly with manual suction pumps in the intertidal region of the sand beach, from May/2005 to September/2006. This project was subdivided into three chapters: Population Structure, Relative growth and Reproductive Strategies. In Chapter I, the population of A. aidae was determined in terms of seasonal abundance, size frequency distribution, sex ratio, density and incidence in the burrows of the thalassinids, reproductive and recruitment periods. In addition, were evaluated the correlations between its density and size with size of their respective hosts. Was found that Austinixa species posses similar biological and ecological aspects, even in distant localities. In Chapter II, due to sexual dimorphism and the morphological adaptations assigned to symbiotic life style, body features, relative growth, maturity and sexual dimorphism were studied. Adults of A. aidae have the carapace width on average 2.4 times the carapace length. Relative growth revealed that morphological changes are related to sexual maturity of this species. In Chapter III, the goal was to know the reproductive strategy related to production of eggs, to determine the fecundity of ovigerous females of different sizes and different periods, conditions of the development of eggs, and their parameters (diameter and volume). A. aidae presented patterns of reproductive strategy similar to other Decapoda crustaceans, with evidence of reproductive efficiency where this population is established. Due to a lack of population and reproductive studies for this species, this study aimed to supply this demand in the scientific literature.
Robeck, Alexandra. « Pseudevernia furfuracea - patterns of diversity in a shrubby lichen ». Thesis, Södertörn University College, School of Life Sciences, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-1320.
Texte intégralPseudevernia furfuracea is a shrubby lichen commonly found around the Baltic Sea. The lichen appears to reproduce mainly by producing isidia, propagules of the lichen cortex. It is very morphologically and chemically diverse and can be found on different substrates, such as nutrient poor bark trees and rocks. The main objective of this study was to describe genetic variation in the ITS region and link this to variations in morphology, substrate ecology and secondary chemistry. In total, 36 specimens of P. furfuracea were collected from 21 sites in Sweden, Finland and Estonia. Seven haplotypes were distinguished in the ITS region. A statistically significant connection between haplotype and colouration of the lichen was found. Lighter coloured specimens of P. furfuracea are more likely to be of the ancestral haplotype. No other significant correlations between the different characteristics studied were found, suggesting that P. furfuracea should be regarded as a single species within the Baltic Sea area. This is also supported by the haplotype network.
Parker, Timothy P. « Integrating Concepts in Modern Molecular Biology into a High School Biology Curriculum ». Thesis, University of North Texas, 2003. https://digital.library.unt.edu/ark:/67531/metadc4255/.
Texte intégralLau, Katherine Aik Hee. « Biology and molecular biology of new HIV-1 recombinant forms from Malaysia ». Connect to full text, 2008. http://hdl.handle.net/2123/4129.
Texte intégralTitle from title screen (viewed 31 March 2009). Submitted in fulfilment of the of the requirements for the degree of Doctor of Philosophy to the Discipline of Medicine, Faculty of Medicine. Degree awarded 2009; thesis submitted 2008. Includes bibliographical references. Also available in print form.
Rozga, Michele E. « The Old Biology Book ». Digital Archive @ GSU, 2011. http://digitalarchive.gsu.edu/english_diss/68.
Texte intégralRoth, Kirk L. « Cerulean warbler breeding biology ». Virtual Press, 2004. http://liblink.bsu.edu/uhtbin/catkey/1292543.
Texte intégralDepartment of Biology
Collins, Raymond P. « Counterproliferation of biology weapons ». Thesis, Monterey, California. Naval Postgraduate School, 1995. http://hdl.handle.net/10945/7561.
Texte intégralDissanayake, N. P. « Studies on weed biology ». Thesis, University of Reading, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332797.
Texte intégralRodd, R. A. « Biology, ethics and animals ». Thesis, Open University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377940.
Texte intégralVolta, Manuela. « Functional biology of neurofibromin ». Thesis, King's College London (University of London), 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409315.
Texte intégralStevenson, Mark. « Geographic profiling in biology ». Thesis, Queen Mary, University of London, 2013. http://qmro.qmul.ac.uk/xmlui/handle/123456789/9061.
Texte intégralREZENDE, PATRICIA ALMEIDA DE. « MULTIMODALITY IN BIOLOGY TEXTBOOKS ». PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2004. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=5841@1.
Texte intégralOs livros didáticos modernos constituem-se num objeto de estudo valioso para o lingüista por serem elaborados a partir de múltiplas modalidades semióticas e porque, em geral, são gêneros disciplina-específicos. Entretanto, no que tange à disciplina de Biologia, ainda há poucos estudos que exploram a interação entre as modalidades verbal e visual nos livros de Ensino Médio e, até o momento, nenhum trabalho de natureza contrastiva foi realizado no Brasil sobre esse aspecto. Na presente análise, investigam-se os tipos de representação visual e o modo de estabelecimento da relação entre figuras e o texto verbal associado em dois livros didáticos de Biologia do Ensino Médio, um brasileiro e outro norteamericano. Este estudo baseia-se principalmente nos pressupostos teóricos de Kress e van Leeuwen (1996, 2001) e inspira-se nos trabalhos de Myers (1997) e Nascimento (2002). Implícita nesses pressupostos está a abordagem sistêmicofuncional de Halliday e Hasan (1976) e de Halliday (1994). Os resultados desta pesquisa revelam que, dentre outros mecanismos, a coesão lexical exerce importante papel na interação entre o componente verbal das figuras, o texto principal e as legendas em ambos os livros analisados. Dentre as diferenças encontradas nos dois livros, destacam-se as representações visuais distintas referentes a um mesmo tópico, o modo de referenciação às figuras no texto, a função das legendas e a proporção de utilização de termos técnicos na coesão intermodal. Esses resultados mostram que existem variações no gênero livro didático de Biologia quanto ao modo de utilização dos mecanismos de interação entre figuras e texto verbal, que podem estar relacionadas com os propósitos dos autores. Isso pode ter importantes implicações para o ensino da Biologia e de Inglês para fins específicos, pois auxilia na conscientização de educadores e alunos sobre a necessidade de se levar em consideração os meios de interação entre as linguagens visual e verbal na interpretação e produção dos textos multimodais próprios de cada comunidade discursiva.
Modern textbooks are valuable as a research object in applied linguistics because they usually present their content through multiple semiotic modalities and because in general they are discipline-specific genres. However, concerning the discipline of Biology, there are still few studies that exploit the interaction between verbal and visual languages in highschool textbooks and, up to the moment, no contrastive works on this topic have been published in Brazil. For this reason, the goal of the present study is to investigate the types of visual representations and the strategies that establish the interaction between pictures and verbal text used in two highschool-level Biology textbooks, a Brazilian and a North-American one. This study is based mainly on Kress and van Leeuwen s (1996, 2001) theory of multimodality and is inspired in Myers (1997) and Nascimento s (2002) previous works. This theoretical basis presupposes Halliday and Hasan s (1976) and Halliday s (1997) systemic- functional approach to text. The results of this investigation reveal that, among other mechanisms, lexical cohesion has an important role in the interaction between the verbal element in images and the verbal language of the corresponding texts and captions in both books. Among the differences found between these two books, there are the different visual representations concerning the same topic, the way pictures are referred to in the text, the role of the captions and the proportion of technical terms used in intermodal cohesion. These results show that there are intrageneric variations in the use of such resources that are probably due to the authors purposes. The findings may have important implications for the teaching of Biology and of English for specific purposes. They may enhance the awareness of educators and students about the need of taking the interaction between visual and verbal languages into consideration for the appropriate interpretation of texts and for the production of multimodal texts specific to each discursive community.
Skinner, Richard. « Structural biology of antithrombin ». Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627475.
Texte intégralSioson, Allan A. « Multimodal Networks in Biology ». Diss., Virginia Tech, 2005. http://hdl.handle.net/10919/29995.
Texte intégralPh. D.
Thanthianga, Clement. « Biology of Callosobruchus maculatus / ». The Ohio State University, 1985. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487264603217199.
Texte intégralRamsden, Sean. « Intelligent design and biology ». Thesis, Rhodes University, 2003. http://hdl.handle.net/10962/d1007561.
Texte intégralKMBT_363
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Šarić, Jasmin. « Extracting information for biology ». [S.l. : s.n.], 2006. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-27959.
Texte intégralMazzag, Barbara Cathrine. « Mathematical models in biology / ». For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2002. http://uclibs.org/PID/11984.
Texte intégralOwens, Nick D. L. « From biology to algorithms ». Thesis, University of York, 2010. http://etheses.whiterose.ac.uk/1380/.
Texte intégralBoland, Howard. « Art from synthetic biology ». Thesis, University of Westminster, 2013. https://westminsterresearch.westminster.ac.uk/item/8yyq5/art-from-synthetic-biology.
Texte intégralSowell, Mitzie Leigh Eick Charles Joseph. « The relationship of parental influence on student career choice of biology and non-biology majors enrolled in a freshman biology course ». Auburn, Ala., 2006. http://repo.lib.auburn.edu/2006%20Fall/Dissertations/SOWELL_MITZIE_10.pdf.
Texte intégralAderhold, Andrej. « Machine learning in systems biology at different scales : from molecular biology to ecology ». Thesis, University of St Andrews, 2015. http://hdl.handle.net/10023/7030.
Texte intégralTempa, Tshering. « Teaching wildlife biology in Bhutan development of wildlife biology curriculum and teaching modules / ». CONNECT TO THIS TITLE ONLINE, 2008. http://etd.lib.umt.edu/theses/available/etd-10212008-220358/.
Texte intégralHorne, Elaine. « Biology teachers’ use of their interpretational frameworks for assessing students’ understandings in biology ». Thesis, Curtin University, 2015. http://hdl.handle.net/20.500.11937/1817.
Texte intégral