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Articles de revues sur le sujet "Binding, remnants used in"
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Borensztajn, J., T. J. Kotlar et C. A. Matza. « Heparin-binding apoproteins. Effects on lipoprotein lipase and hepatic uptake of remnants ». Biochemical Journal 233, no 3 (1 février 1986) : 909–12. http://dx.doi.org/10.1042/bj2330909.
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Apoprotein-free heparin-binding and non-binding chylomicrons were used as substrates to test the effects on lipoprotein lipase activity of (a) chylomicron protein I; (b) the mixture of proteins I, II and apoprotein E and (c) human beta 2-glycoprotein I. No activation of the enzyme was observed with any of those apoproteins. When rats were injected simultaneously with [3H]cholesterol-labelled heparin-binding chylomicrons (containing proteins I and II) and [14C]cholesterol-labelled non-binding chylomicrons, no differences were detected between the rates of removal from circulation of those two types of particles. Clearance of chylomicrons from circulation was accompanied by the incorporation of 3H and 14C labels into the livers at similar rates. It is concluded that proteins I, II and apoprotein E have no effect on the degradation of chylomicrons by lipoprotein lipase and that the hepatic recognition of remnants does not appear to be affected by proteins I and II.
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Boullé, Mikaël, Laurianne Davignon, Keïs Nabhane Saïd Halidi, Salomé Guez, Emilie Giraud, Marcel Hollenstein et Fabrice Agou. « High-Content RNAi Phenotypic Screening Unveils the Involvement of Human Ubiquitin-Related Enzymes in Late Cytokinesis ». Cells 11, no 23 (30 novembre 2022) : 3862. http://dx.doi.org/10.3390/cells11233862.
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CEP55 is a central regulator of late cytokinesis and is overexpressed in numerous cancers. Its post-translationally controlled recruitment to the midbody is crucial to the structural coordination of the abscission sequence. Our recent evidence that CEP55 contains two ubiquitin-binding domains was the first structural and functional link between ubiquitin signaling and ESCRT-mediated severing of the intercellular bridge. So far, high-content screens focusing on cytokinesis have used multinucleation as the endpoint readout. Here, we report an automated image-based detection method of intercellular bridges, which we applied to further our understanding of late cytokinetic signaling by performing an RNAi screen of ubiquitin ligases and deubiquitinases. A secondary validation confirmed four candidate genes, i.e., LNX2, NEURL, UCHL1 and RNF157, whose downregulation variably affects interconnected phenotypes related to CEP55 and its UBDs, as follows: decreased recruitment of CEP55 to the midbody, increased number of midbody remnants per cell, and increased frequency of intercellular bridges or multinucleation events. This brings into question the Notch-dependent or independent contributions of LNX2 and NEURL proteins to late cytokinesis. Similarly, the role of UCHL1 in autophagy could link its function with the fate of midbody remnants. Beyond the biological interest, this high-content screening approach could also be used to isolate anticancer drugs that act by impairing cytokinesis and CEP55 functions.
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Mamo, John C. L., Gerald F. Watts, P. Hugh R. Barrett, Darrin Smith, Anthony P. James et Sebely Pal. « Postprandial dyslipidemia in men with visceral obesity : an effect of reduced LDL receptor expression ? » American Journal of Physiology-Endocrinology and Metabolism 281, no 3 (1 septembre 2001) : E626—E632. http://dx.doi.org/10.1152/ajpendo.2001.281.3.e626.
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Postprandial lipemia after an oral fat challenge was studied in middle-aged men with visceral obesity. The two groups had similar plasma cholesterol levels, but obese subjects had higher levels of plasma triglyceride and reduced amounts of high-density cholesterol. Fasting plasma insulin was fourfold greater in obese subjects because of concomitant insulin resistance, with a calculated HOMA score of 3.1 ± 0.6 vs. 0.8 ± 0.2, respectively. Plasma apolipoprotein B48(apoB48) and retinyl palmitate (RP) after an oral fat challenge were used to monitor chylomicron metabolism. Compared with lean subjects, the fasting concentration of apoB48 was more than twofold greater in obese individuals, suggestive of an accumulation of posthydrolyzed particles. After the oral lipid load, the incremental areas under the apoB48 and RP curves (IAUC) were both significantly greater in obese subjects (apoB48: 97 ± 17 vs. 44 ± 12 μg · ml−1 · h; RP: 3,120 ± 511 vs. 1,308 ± 177 U · ml−1 · h, respectively). A delay in the conversion of chylomicrons to remnants probably contributed to postprandial dyslipidemia in viscerally obese subjects. The triglyceride IAUC was 68% greater in obese subjects (4.7 ± 0.6 vs. 2.8 ± 0.8 mM · h, P< 0.06). Moreover, peak postprandial triglyceride was delayed by ∼2 h in obese subjects. The reduction in triglyceride lipolysis in vivo did not appear to reflect changes in hydrolytic enzyme activities. Postheparin plasma lipase rates were found to be similar for lean and obese subjects. In this study, low-density lipoprotein (LDL) receptor expression on monunuclear cells was used as a surrogate marker of hepatic activity. We found that, in obese subjects, the binding of LDL was reduced by one-half compared with lean controls (70.9 ± 15.07 vs. 38.9 ± 4.6 ng LDL bound/μg cell protein, P = 0.02). Because the LDL receptor is involved in the removal of proatherogenic chylomicron remnants, we suggest that the hepatic clearance of these particles might be compromised in insulin-resistant obese subjects. Premature and accelerated atherogenesis in viscerally obese, insulin-resistant subjects may in part reflect delayed clearance of postprandial lipoprotein remnants.
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Zheng, Yifan, Aidan F. Pierce, Willi L. Wagner, Hassan A. Khalil, Zi Chen, Andrew B. Servais, Maximilian Ackermann et Steven J. Mentzer. « Functional Adhesion of Pectin Biopolymers to the Lung Visceral Pleura ». Polymers 13, no 17 (2 septembre 2021) : 2976. http://dx.doi.org/10.3390/polym13172976.
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Pleural injuries and the associated “air leak” are the most common complications after pulmonary surgery. Air leaks are the primary reason for prolonged chest tube use and increased hospital length of stay. Pectin, a plant-derived heteropolysaccharide, has been shown to be an air-tight sealant of pulmonary air leaks. Here, we investigate the morphologic and mechanical properties of pectin adhesion to the visceral pleural surface of the lung. After the application of high-methoxyl citrus pectin films to the murine lung, we used scanning electron microscopy to demonstrate intimate binding to the lung surface. To quantitatively assess pectin adhesion to the pleural surface, we used a custom adhesion test with force, distance, and time recordings. These assays demonstrated that pectin–glycocalyceal tensile adhesive strength was greater than nanocellulose fiber films or pressure-sensitive adhesives (p < 0.001). Simultaneous videomicroscopy recordings demonstrated that pectin–glycocalyceal adhesion was also stronger than the submesothelial connective tissue as avulsed surface remnants were visualized on the separated pectin films. Finally, pleural abrasion and hyaluronidase enzyme digestion confirmed that pectin binding was dependent on the pleural glycocalyx (p < 0.001). The results indicate that high methoxyl citrus pectin is a promising sealant for the treatment of pleural lung injuries.
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Sleiderink, Remco, et Ben van der Have. « Een nieuw fragment van handschrift A van de Roman der Lorreinen (Michigan State University, Criminology Collection, XX KJC7690.A48 1687) ». Tijdschrift voor Nederlandse Taal- en Letterkunde 137, no 2 (1 janvier 2021) : 102–21. http://dx.doi.org/10.5117/tntl2021.2.001.slei.
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Abstract Among the many books in Michigan State University’s Criminology Collection is a Corpus juris militaris, published in Germany in 1687. Its binding contains four small parchment strips with medieval Dutch verses. Although the strips are still attached in the spine, the verses can be identified as belonging to the Roman der Lorreinen, and more specifically as remnants of manuscript A, written in the duchy of Brabant in the second quarter of the fourteenth century. Manuscript A originally must have consisted of over 400 leaves, containing more than 150.000 verses (note: there are no complete manuscripts of the Roman der Lorreinen). Only 7% of manuscript A has been preserved in several European libraries, mainly in Germany. The new fragment suggests that manuscript A was used as binding material not earlier than the end of the seventeenth century (after 1687). The newly found verses are from the first part of the Roman der Lorreinen, which was an adaptation of the Old French chanson de geste Garin le Loherenc. This article offers a first edition and study of the verses, comparing them to the Old French counterparts. This comparison offers additional evidence for the earlier hypothesis that manuscript A contained the same adaptation of Garin le Loherenc as the fragmentary manuscripts B and C.
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WENDLING, MIRIAM. « The reception of Hermann of Reichenau at St Michelsberg : some observations on fragments of interval notation ». Plainsong and Medieval Music 24, no 2 (25 septembre 2015) : 113–27. http://dx.doi.org/10.1017/s0961137115000108.
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ABSTRACTThe bindings of a large number of manuscripts formerly held in the library of the Benedictine monastery of St Michelsberg in Bamberg preserve fragments of medieval chantbooks documenting a culture in which practical singing and music theory were closely intertwined. These remnants, the work of generations of scribes, reveal a sustained concern for correctness ranging from the use of pitch-clarifying neume notations, to the use of letters, to the recording of melodies along lines prescribed in theoretical works. The influence the music theorist Hermann of Reichenau on St Michelsberg's own theorist, Frutolf, raises two questions. First, what is the nature of the relationship between practical chant and music theory books at St Michelsberg? Second, how was Hermann's notation used at the monastery?
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Doungudomdacha, Sombhun, Alla Volgina et Joseph M. DiRienzo. « Evidence that the cytolethal distending toxin locus was once part of a genomic island in the periodontal pathogen Aggregatibacter (Actinobacillus) actinomycetemcomitans strain Y4 ». Journal of Medical Microbiology 56, no 11 (1 novembre 2007) : 1519–27. http://dx.doi.org/10.1099/jmm.0.47273-0.
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The authors have previously shown that the periodontal pathogen Aggregatibacter (formerly Actinobacillus) actinomycetemcomitans Y4 contains an operon for a genotoxin known as the cytolethal distending toxin (Cdt). The cdt locus in strain Y4 is flanked by remnants of heterologous plasmid and integrase sequences. In this study, the DNA sequence immediately downstream from the cdt locus on the Y4 chromosome was examined. The extended sequence contained a region that had all the characteristics of a typical bacterial pathogenicity or genomic island. The genomic island (GIY4-1) was approximately 22 kb long, was flanked by a bacteriophage attachment (att) sequence and contained a full-length integrase/resolvase gene (xerD). A total of 22 complete and partial ORFs represented putative DNA replication/DNA binding/conjugation proteins as well as hypothetical proteins. GIY4-1 was most closely related to putative genomic islands in Haemophilus ducreyi 35000HP and Haemophilus influenzae 86-028NP and to a chromosomal region in Haemophilus somnus 129PT. GIY4-1 was not present in HK1651, which was used as the prototype strain for genomic sequencing of A. actinomycetemcomitans. Several sequences in GIY4-1 were homologous to ORFs found on the A. actinomycetemcomitans plasmid pVT745. None of the identified ORFs in GIY4-1 appeared to encode potential virulence genes. However, several unique observations supported the possibility that the cdt locus of A. actinomycetemcomitans Y4 was originally contained within the genomic island.
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Pawar, Vipul Mohan, Rashmi Hosalkar et Janaki Iyer. « Immunohistochemical Evaluation of Calretinin and Cytokeratin-19 in Odontogenic Keratocyst and Ameloblastoma : A Retrospective Study ». Journal of Contemporary Dentistry 5, no 2 (2015) : 98–103. http://dx.doi.org/10.5005/jp-journals-10031-1116.
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ABSTRACT The odontogenic epithelial remnants, i.e. cell rests of Serre and Malassez, are formed from dental lamina and Hertwig's epithelial root sheath respectively, may proliferate and have role in pathogenesis of odontogenic cysts and tumors. Odontogenic keratocyst (OKC) is the most common and aggressive cyst of the dental lamina origin. Ameloblastoma, the second most common odontogenic tumor (OT), is a clinically benign and locally invasive polymorphic neoplasia. Differentiation of OKC from ameloblastoma sometimes poses a diagnostic dilemma, thus necessitating the need to differentiate between the two (especially unicystic ameloblastoma and OKC). Calretinin, a calcium binding protein, functions as a calcium buffer and a regulator of apoptosis. Some studies have shown its expression in parakeratinized OKC, unicystic and solid ameloblastoma, but not in other OTs. Calretinin may thus provide a better understanding of the biological behavior and tumorogenesis of ameloblastoma. cytokeratin (CK)-19 is a type I cytokeratin, has been found to be a reliable marker of epithelial differentiation. The intense expression of CK-19 is useful for identification of odontogenic epithelial components, thus suggesting their potential for proliferation to form epithelial odontogenic cysts and tumors. The aim of this study is to evaluate calretinin and Ck-19 in OKC and ameloblastoma. For this retrospective study, 20 formalin fixed paraffin embedded tissue samples of histopathologically proven OKC and ameloblastoma each, retrieved from the department of oral pathology was used. The results will be evaluated by using immunohistochemical analysis. How to cite this article Pawar VM, Patel S, Pathak J, Swain N, Hosalkar R, Iyer J. Immunohistochemical Evaluation of Calretinin and Cytokeratin-19 in Odontogenic Keratocyst and Ameloblastoma: A Retrospective Study. J Contemp Dent 2015;5(2):98-103.
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Watson, T. D., C. E. Tan, M. McConnell, S. K. Clegg, L. F. Squires et C. J. Packard. « Measurement and physiological significance of lipoprotein and hepatic lipase activities in preheparin plasma ». Clinical Chemistry 41, no 3 (1 mars 1995) : 405–12. http://dx.doi.org/10.1093/clinchem/41.3.405.
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Abstract A radiochemical method for selective measurement of postheparin lipase activities was adapted to analyze lipoprotein lipase and hepatic lipase in preheparin plasma. The assay sensitivity was increased about four-fold by doubling both the volume of plasma used and the volume of lipolytic products taken for liquid scintillation counting, and was further improved by increasing the incubation period by 50% to 90 min. Rabbit antiserum to human hepatic lipase was unsuitable for the selective measurement of lipoprotein lipase because of apparent endogenous lipolytic activity. Preheparin hepatic lipase, however, was sensitive to inactivation by sodium dodecyl sulfate (SDS), the inhibition being greatest (> 90%) for plasma incubated with an equal volume of 40 mmol/L SDS. Intra- and interassay CVs for the two enzymes were 12.5-14.6% and 17.4-19.7%, respectively. In a cross-sectional study of 84 healthy subjects, pre- and postheparin hepatic lipase activities were higher in men than women, were correlated with indices of obesity, and were significantly correlated with one another, which explained the association of the former with plasma concentrations of high-density lipoprotein (HDL), HDL2, and small, dense low-density lipoproteins. There was no significant relationship between pre- and postheparin lipoprotein lipase activities, but the former were correlated with plasma concentrations of free fatty acids (FFA) and very-low-density lipoprotein. Apparently, preheparin activities of hepatic lipase, but not of lipoprotein lipase, may be a useful measure of the physiological function of "whole body" enzyme activity in cross-sectional and metabolic studies, where heparinization is not possible. Preheparin lipoprotein lipase activities, however, may reflect displacement of the enzyme by FFA and subsequent binding to remnants of triglyceride-rich lipoproteins.
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Windler, E., J. Greeve, B. Levkau, V. Kolb-Bachofen, W. Daerr et H. Greten. « The human asialoglycoprotein receptor is a possible binding site for low-density lipoproteins and chylomicron remnants ». Biochemical Journal 276, no 1 (15 mai 1991) : 79–87. http://dx.doi.org/10.1042/bj2760079.
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Binding and internalization of chylomicron remnants from rat mesenteric lymph by HepG2 cells was inhibited by both excess remnants and low-density lipoprotein (LDL) to the same extent. Ligand blots revealed binding of remnants and LDL to the LDL receptor. Measures regulating LDL receptor activity greatly influenced the binding of remnants: ethinyloestradiol, the hydroxymethylglutaryl-CoA reductase inhibitor pravastatin and the absence of LDL all increased binding, whereas high cell density or the presence of LDL decreased binding. Also, asialofetuin, asialomucin, the neoglycoprotein galactosyl-albumin and an antibody against the asialoglycoprotein receptor all decreased substantially the binding of remnants. At high cell density, binding internalization and degradation of chylomicron remnants was inhibited by up to 70-80%, yet binding of LDL was inhibited by no more than 20-30%. In cross-competition studies, the binding of 125I-asialofetuin was efficiently competed for by asialofetuin itself or by the antibody, and also by LDL and remnants, yet remnants displayed an approx. 100-fold higher affinity than LDL. Likewise, remnants of human triacylglycerol-rich lipoproteins and asialofetuin interfered with each others' binding to HepG2 cells or human liver membranes. It is concluded that the LDL receptor mediates the internalization of chylomicron remnants into hepatocytes depending on its activity, according to demand for cholesterol. Additionally, the asialoglycoprotein receptor may contribute to the endocytosis of LDL, but predominantly of chylomicron remnants.
Thèses sur le sujet "Binding, remnants used in"
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Jones, Angel D. Mrs. « Remnants ». VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/4006.
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Angel Jones
Artist Statement
My mixed media collages address issues concerning the outcast, the overlooked, and the underprivileged. My inspirations come from what I experience and perceive around me. My purpose in life is to use my art to address social issues that involve children. For example, I focus my attention on issues ranging from homelessness to mental illness. I am passionate about how vital these concerns are to our success as human beings.
To express my ideas, I layer a variety of materials and textiles. The materials include fragments of photographs, drawings, and objects. The textiles include articles of clothing and fabrics. Recurring symbols and imagery link the individual pieces together. The theme of each work determines the materials and media. While doing research for a piece, new social issues often surface and lead to the next piece.
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Knight, Derek Andrew. « Preparation of a site-specific lymphotoxin- mutant to be used in protein characterization and receptor binding studies ». CSUSB ScholarWorks, 1995. https://scholarworks.lib.csusb.edu/etd-project/987.
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Eveness, John Richard William. « Investigation into the response of a resonant coil magnetometer detecting paramagnetic particles used as lables in biological binding assays ». Thesis, University of the West of England, Bristol, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.444486.
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Vashisht, S. « COMPUTATIONAL APPROACHES IN THE ESTIMATION AND ANALYSIS OF TRANSCRIPTS DIFFERENTIAL EXPRESSION AND SPLICING : APPLICATION TO SPINAL MUSCULAR ATROPHY ». Doctoral thesis, Università degli Studi di Milano, 2017. http://hdl.handle.net/2434/470076.
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Spinal Muscular Atrophy (SMA) is among the most common genetic neurological diseases that cause infant mortality. SMA is caused by deletion or mutations in the survival motor neuron 1 gene (SMN1), which are expected to generate alterations in RNA transcription, or splicing and most importantly reductions in mRNA transport within the axons of motor neurons (MNs). SMA ultimately results in the selective degeneration of MNs in spinal cord, but the underlying reason is still not clear entirely. The aim of this study is to investigate splicing abnormalities in SMA, and to identify genes presenting differential splicing possibly involved in the pathogenesis of SMA at genome-wide level. We performed RNA-Sequencing data analysis on 2 SMA patients and 2 controls, with 2 biological replicates each sample, derived from their induced Pluripotent Stem Cells-differentiated-MNs. Three types of analyses were executed. Firstly, differential expression analysis was performed to identify possibly mis-regulated genes using Cufflinks. Secondly, alternative splicing analysis was conducted to find differentially-used exons (DUEs; using DEXSeq) as splicing patterns are known to be altered in MNs by the suboptimal levels of SMN protein. Thirdly, we did RNA-binding protein (RBP) - motif discovery for the set of identified alternative cassette-DUEs, to pinpoint possible mechanisms of such alterations, specific to MNs. The gene ontology enrichment analysis of significant DEGs and alternative cassette-DUEs revealed various interesting terms including axon-guidance, muscle-contraction, microtubule-based transport, axon-cargo transport, synapse etc. which suggests their involvement in SMA. Further, promising results were obtained from motif analysis which has identified 22 RBPs out of which 7 RBPs namely, PABPC1, PABPC3, PABPC4, PABPC5, PABPN1, SART3 and KHDRBS1 are known for mRNAs stabilization and mRNA transport across MN-axon. Five RBPs from PABP family are known to interact directly with SMN protein that enhance mRNA transport in MNs. To validate our results specific wet-lab experiments are required, involving precise recognition of RNA-binding sites correspondent with our findings. Our work has provided a promising set of putative targets which might offer potential therapeutic role towards treating SMA.
During the course of our study, we have observed that current methods for an effective understanding of differential splicing events within the transcriptomic landscape at high resolution are insufficient. To address this problem, we developed a computational model which has a potential to precisely estimate the “transcript expression levels” within a given gene locus by disentangling mature and nascent transcription contributions for each transcript at per base resolution. We modeled exonic and intronic read coverages by applying a non-linear computational model and estimated expression for each transcript, which best approximated the observed expression in total RNA-Seq data. The performance of our model was good in terms of computational processing time and memory usage. The application of our model is in the detection of differential splicing events. At exon level, differences in the ratio of the sum of mature and the sum of nascent transcripts over all the transcripts in a gene locus gives an indication of differential splicing. We have implemented our model in R-statistical language.
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« The exploration of the binding capabilities of perfluoropentane microdroplets and microbubbles used in acoustic droplet vaporization ». Tulane University, 2020.
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archives@tulane.eduAcoustic droplet vaporization (ADV) is an attractive alternative to traditional hepatocellular carcinoma (HCC) treatments. ADV involves injecting microdroplets into the bloodstream which then accumulate in and around the tumor’s vasculature. Once accumulated, high-power ultrasound is used to vaporize the microdroplets into larger perfluoropentane gas microbubbles which occlude blood flow and induce necrosis of the tumor without harming healthy tissue like traditional HCC treatments. This study aims to optimize ADV treatment by improving the shell composition and surface architecture of microdroplets while ensuring the treatment remains safe. In order to ensure the treatment is as effective as possible, the microdroplets must have powerful binding capabilities, guaranteeing maximum microdroplet accumulation and treatment efficacy. The binding capabilities of three microdroplet shell compositions, created by adjusting the molar percentages of the three lipids found in the shell, were investigated and found to all have equal binding abilities. The surface architecture of these microdroplets were also altered to maximise binding capabilities. Microdroplets can have either an exposed-ligand or buried-ligand surface architecture. In microdroplets with a buried-ligand surface architecture, the attached tumor-targeting ligands are hidden within a layer of longer lipid chains which allow the microdroplets to evade the immune system and circulate within the bloodstream longer, increasing treatment efficacy. It was found that microdroplets with a buried-ligand surface architecture do not have comparable binding capabilities to microdroplets with an exposed-ligand surface architecture and are therefore not a viable alternative for use in ADV. Finally, the velocity required to dislodge perfluoropentane gas microbubbles was explored to determine if the gas microbubbles can remain adhered to the tumor’s vasculature to create a strong occlusion. Since perfluoropentane gas microbubbles occlude blood flow it is imperative that the microbubbles remain in the tumor’s vasculature and do not dislodge and accumulate in other parts of the body’s vasculature. By measuring the velocity and calculating the force necessary for dislodgement and comparing those values to those found in capillaries it was concluded that the perfluoropentane gas microbubbles can withstand the force of blood flow and remain lodged in capillaries.
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Chloe Celingant-Copie
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Lee, Wen-Yuan, et 李文元. « The Studies on The Stability of the Binding Media of the Retouching Materials Used on Exterior Painted Wooden Decoration in Taiwan ». Thesis, 2011. http://ndltd.ncl.edu.tw/handle/78404816297168812516.
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碩士國立臺南藝術大學
博物館學與古物維護研究所
99
Retouching material is the paint to fill the lacuna of color on art works during conservation process. The concept and technique of retouching from the western countries are more developed and comprehensive, the over viewing of the developing history and the material evolvment since hundreds centuries reveals the foundation of conservation wasn’t simply built in oe day. The binder of retouching material holds the key to determine the physical, chemical and photochemical properties, which also closely bonded with the handling ability and environmental condition. Owing to the fact of higher stability and easy to be modifying, the study of retouching binder leads toward the direction of synthetic resin. Based by the western conservation concept and technique, the conservation in Taiwan is striding forward to the international level. However, if the material that has been tested by museum condition can be directly transfer to the island climate of Taiwan is still a question of uncertain. Thus, this study choose three of the most recommended retouching material (1)watercolor coated with 5% acrylic resin Paraloid® B72, (2)acrylic resin Paraloid® B72 and (3)low molecule weight resin Laropal® A81 to proceed accelerate aging process with the simulation of Taiwan’s exterior condition, and measure the alter of color, gloss, adhesion and solubility to evaluate the stability and reversibility of the materials. The results shows that both the water color coated 5% Paraloid® B72 and Laropal® A81 have lower resistance against moisture, thus are not recommended for the use of exterior area. The stability and reversibility of Paraloid® B72 after aged is durable, and when the physical damages such as hollow and flaking occurred, the consolidation is achievable simply with pressure and heat. But the material is more suitable for hatching technigue rather than paint large area at once.
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Lee, Chuna-Chieh, et 李泉杰. « The effects and the influences of the metal braid binding modes of HDMI cables commonly used in audio and video systems ». Thesis, 2013. http://ndltd.ncl.edu.tw/handle/08784510947828979886.
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碩士中原大學
電子工程研究所
101
Abstract The purpose of this study focuses mainly on the investigation of HDMI cables that are presently used in the audio and video communications, the ways of metal braid closeout patterns at the ends and the ground areas of HDMI cables; as well as the best results for the suppression of interfering electromagnetic radiation of EMI. The reason why we choose this subject as the interests and the thesis for our study is mainly because of the increasing emphasis trend on the problems of electromagnetic interferences and the hazards as well as the impacts caused for human bodies of the modern life. As far as the current HDMI product manufacturing, most of the studies focus on the applications of cable materials and it is rare for such formal tests. Therefore, in this study we used the standard EMI laboratory and measuring devices to measure and to analyze the commonly used HDMI cables of different ground areas and sizes. With the actual measurements and analyses, we tried to find out the shielding effects of electromagnetic radiation interference that are presented by different cable metal braids under the standard 30MHz ~ 1GHz measuring conditions of the HDMI cables. With the experiments, we found that the metal braids inside the HDMI cables with larger ground areas showed better shielding effects of electromagnetic radiation interference than those with smaller ground areas. We hope that, with the investigations and the results of this study, we might provide some personal opinions for those experts of the industries who are interested in the research and the understanding of this field.
Livres sur le sujet "Binding, remnants used in"
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David, Rundle, et Mandelbrote Scott, dir. Fragments of medieval manuscripts used as pastedowns in Oxford bindings : With a survey of Oxford binding c. 1515-1620. Oxford : Printed for the Oxford Bibliographical Society, 2004.
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Daniel, Epstein, et Eastern Region Road Maintenance Project (Nepal), dir. Bio-engineering for road protection in Nepal : Techniques used on the Jogbani-Basantapur road. [Kathmandu?] : Eastern Region Road Maintenance Project, 1993.
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Institute, Library Binding, dir. Long-term service life and performance characteristics of the PVA cold emulsion adhesives used for the double fanning process in library binding : Are PUR adhesives a viable alternative ? [Rochester, N.Y.] : Library Binding Institute, 1991.
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Vickers, Dr Glen A. Faith and Belief : Remnants of Our Ancestry Used to Enslave Our Minds. CreateSpace Independent Publishing Platform, 2016.
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Rolph, John, Elizabeth Rolph et Erik Moller. Binding Arbitration is Not Frequently Used to Resolve Health Care Disputes. RAND Corporation, 1999. http://dx.doi.org/10.7249/rb9030.
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Zaehnsdorf, Joseph William. A Short History Of Bookbinding : And A Glossary Of Styles And Terms Used In Binding. Kessinger Publishing, LLC, 2009.
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The Unknown Masterpiece : Signed Limited Edition of 50 Copies with Original Paintings Used for the Binding. Enitharmon Press, 1996.
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Baauw, Sergio. The Acquisition of Binding and Coreference. Sous la direction de Jeffrey L. Lidz, William Snyder et Joe Pater. Oxford University Press, 2016. http://dx.doi.org/10.1093/oxfordhb/9780199601264.013.22.
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In this chapter the acquisition of pronouns and reflexives is discussed. It reviews several accounts of the so-called Delay of Principle B Effect, the absence of this effect in some languages, and the structural factors that influence its appearance in child language. It also discusses children’s alledged target-like performance on reflexives in several languages with different type of reflexives. The chapter concludes that provided a balanced experimental design is used, the experimental results point at early mastery of Principle A and B, and that children’s difficulties with the interpretation of pronouns and reflexives are to be found at the interfaces between syntax and discourse or semantics, and may be due to limited (syntactic) processing resources.
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Printer's Dictionary of Technical Terms ; a Handbook of Definitions and Information about Processes of Printing ; with a Brief Glossary of Terms Used in Book Binding. Creative Media Partners, LLC, 2022.
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Stewart, A. A. Printer's Dictionary of Technical Terms ; a Handbook of Definitions and Information about Processes of Printing ; with a Brief Glossary of Terms Used in Book Binding. Creative Media Partners, LLC, 2022.
Trouver le texte intégralChapitres de livres sur le sujet "Binding, remnants used in"
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Sultan, Fabrice, Dominique Lagrange et Sabine Griglio. « In Vitro Binding and in Vivo Uptake of Chylomicron Remnants after their Hydrolysis by Hepatic Lipase ». Dans Hypercholesterolemia, Hypocholesterolemia, Hypertriglyceridemia, in Vivo Kinetics, 311–17. Boston, MA : Springer US, 1990. http://dx.doi.org/10.1007/978-1-4684-5904-3_37.
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Pfeifer, K., et K. Oeggl. « Analysis of the bast used by the Iceman as binding material ». Dans The Iceman and his Natural Environment, 69–76. Vienna : Springer Vienna, 2000. http://dx.doi.org/10.1007/978-3-7091-6758-8_6.
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Hood, William F. « Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site ». Dans Methods in Molecular Biology, 121–29. Totowa, NJ : Humana Press, 2010. http://dx.doi.org/10.1007/978-1-59745-364-6_10.
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Dirisu, J. O., O. S. I. Fayomi et S. O. Oyedepo. « Efficacy of Quasi Agro Binding Fibre on the Hybrid Composite Used in Advance Application ». Dans Lecture Notes in Mechanical Engineering, 565–74. Singapore : Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5753-8_52.
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Mayer, Philip, et Andreas Schroeder. « Automated Multi-Language Artifact Binding and Rename Refactoring between Java and DSLs Used by Java Frameworks ». Dans ECOOP 2014 – Object-Oriented Programming, 437–62. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-662-44202-9_18.
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Shulkin, Barry, et Thomas Neil Pascual. « Thyroid ». Dans A Practical Guide for Pediatric Nuclear Medicine, 57–74. Berlin, Heidelberg : Springer Berlin Heidelberg, 2023. http://dx.doi.org/10.1007/978-3-662-67631-8_5.
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AbstractThyroid imaging, one of the earliest scanning tests, is used in children to determine the cause of congenital and acquired thyroid diseases. It should be interpreted in conjunction with the patient’s clinical picture, thyroid function tests, history of medication and diet, physical examination, thyroid function tests, and ultrasound. Congenital hypothyroidism (CHT) is usually detected on newborn screening. Ectopic thyroid and thyroid aplasia may result in hypothyroidism requiring lifelong hormone replacement therapy. In the presence of a normally positioned, fully developed thyroid, CHT can be transient. Evaluation of acquired benign thyroid disease in children is occasionally supplemented by radioactive iodine uptake tests and can be an aid in therapeutic decisions. Thyroid cancer is the most common endocrine malignancy in children and represents up to 2% of all cancers diagnosed by the age of 20 years, more common in teenagers. It is diagnosed in 25% of thyroid nodules in children, as compared to 10–15% in adults. Children with thyroid cancer present with more advanced disease than adults, including multifocal primary and a higher incidence of regional and distant metastases. Cervical lymph node metastases are diagnosed in 50% and lung metastases in 20% of cases. 131I, a suboptimal agent for imaging, has been largely replaced by 123I and 99mTc-pertechnetate. 131I is at present used mainly for therapeutic purposes in hyperthyroidism, ablation of thyroid remnants following thyroidectomy, and for treatment of thyroid cancer.
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Beers, Erna, et Annelies Jaeken. « Effects on Sexuality of Medication Used in Pregnancy and Childbirth ». Dans Midwifery and Sexuality, 217–29. Cham : Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-18432-1_19.
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AbstractThis chapter focuses on the sexual side effects of various medications used in midwifery and obstetric practice. The chapter will start with background information on pharmacokinetics and pharmacodynamics geared to pregnancy and the changing pregnant body. During pregnancy, the increased cardiac output causes a shorter time to metabolise and eliminate medication. In addition, the blood plasma volume and the total amount of the ‘watery environment’ increase, causing a lower plasma concentration of hydrophilic drugs. Lipophilic drugs take longer to be eliminated because residing longer in the increased ‘fatty environment’ and not in the blood plasma.During pregnancy, the binding of drugs to plasma proteins decreases as well, meaning that a higher amount of the drug can bind to the target with increased therapeutic or undesired side effects.The chapter will give detailed information on the potential sexual implications of medication.Finally, the chapter will indicate how to deal with actual or potential sexual side effects.It is part of ‘Midwifery and Sexuality’, a Springer Nature open-access textbook for midwives and related healthcare professionals (HCPs).
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Bond, H. M., L. Monaco, R. Cortese et K. E. Howell. « An Apo A-I Protein A Hybrid Used for the Study of HDL Receptor Binding and Endocytosis ». Dans Recent Developments in Lipid and Lipoprotein Research, 92–99. Berlin, Heidelberg : Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-83665-7_13.
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Geist, Brian, Adrienne Clements-Egan et Tong-Yuan Yang. « Monitoring Quality of Critical Reagents Used in Ligand Binding Assays with Liquid Chromatography Mass Spectrometry (LC-MS) ». Dans Protein Analysis using Mass Spectrometry, 107–28. Hoboken, NJ, USA : John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781119371779.ch10.
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Takabe, Hideaki. « Self-Similar Solutions of Compressible Fluids ». Dans Springer Series in Plasma Science and Technology, 149–96. Cham : Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-45473-8_4.
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AbstractStrong shock waves are used to compress and heat any matters in the laboratory. The ablation pressure by intense laser is used to compress even solid matters. In plane geometry, it is easier to design multi-shocks to compress the matters, while it is more beneficial to use the spherical compression. No simple solutions are available to know the trajectories of shocks in one-dimensional spherical symmetry. Here we see several analytical solutions with the self-similar method. The method is to find new governing solution of ordinary differential equation from partial differential fluid equations. The self-similar method is known before the birth of computer.The blast wave is the most famous one. Here, we review the basic method to derive several self-similar solutions allowing the spherical implosion, useful to laser driven implosion. The isobaric solution provides uniform pressure and spark-main fuel structure, and isochoric solution gives us uniform density profile at the maximum compression. It is shown that even including thermal conduction, it is possible to find a solution of ablation structure. This is an extended solution more appropriate compared to the steady state solutions shown in the previous chapter.The blast waves are widely used from laser experiments to supernova remnants (SNRs). SNRs are blast waves driven by the matters exploding by supernova explosion. A self-similar solution with forward and reverse shock waves is found to explain many observation data of SNRs. A numerical simulation shows that the solution of ejecta-driven shock changes from Chevalier’s self-similar solution to the other Sedov-Taylor one. The self-similarity is one of the key physics controlling nonlinear hydrodynamic equations.
Actes de conférences sur le sujet "Binding, remnants used in"
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Kuyas, C., A. Haeberli et P. W. Straub. « SEPARATION OF FIBRINOGEN FRAGMENTS ON GLYPRQARGPROLYS-FRACTOGEL ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642885.
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The tetrapeptide GlyProArgPro, which corresponds to the newly exposed N-terminal sequence of fibrin a-polypeptide chain after the action of thraribin, has a binding site in the C-terminal part of the ;-chain as suggested by several authors. Using Gly-ProArgProLys-Fractogel (GPRPK) chrcmatography we tried to isolate a fibrinogen fragment, obtained with different enzymes and conditions, which includes the binding site for GPRP. Human fibrinogen was digested by plasmin in presence and absence of Ca-ions, and the resulting lysates were applied in 0.05 M triethanolamine (TEA), 0.1 M NaCl pH 7.4 to the GPRPK-Fractogel. The gel was washed extensively with TEA-buffer and the adsorbed protein was eluted with 6M urea in TEA-buffer. The protein containing fractions were analyzed iiununologically with anti-fragment E- and with anti-fragment D antibodies. Human fibrinogen was also digested with endopeptidase Arg-C in O.IM NaHco3 pH 8.0 at 37C over night. The enzyme cleaves fibrinogen to fragments, one of which comprising the y-chain sequence 275-375 which is said to contain the fibrin polymerization site. The Arg C-lysate was chromatographed on GPRPK-Fractogel. All fragments were analyzed by SDS-electrophoresis and by reversed-phase HPLC.Fragment D1 was the only fibrinogen fragment which was adsorbed on GPRPK-Fractogel. All other assayed fibrinogen fragments obtained by enzymatic cleavage, showed no affinity to GPRPK-Fractogel. These results demonstrate that for the binding of GPRP to fibrinogen a conformationally intact γ-chain remnant of the fragment D is required.One step chrcmatography using GPRPK-Fractogel can thus also be used to isolate fragment D1 in high purity fran plasmin lysates of fibrinogen.
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Sobel, J. H., C. A. Thibodeau et R. E. Canfield. « EARLY a CHAIN CROSSLINKING OF PARTIALLY DEGRADED FIBRIN(OGEN) MOLECULES ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643321.
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Previous immunochemical studies have demonstrated that the introduction of a chain crosslinks by Factor XIII begins immediately after fibrin aggregation. While there area data to indicate that regions Aα #241-476 and Aα #518-584 are specifically involved in this early crosslinking process, identification of the exact glutamine and lysine residues that participate in the reaction remains to be determined. In this study monoclonal antibodies, whose specificity for these two regions has been defined (anti-Aα #259-276, F-105; anti-Aα #540-554, F-102), were used to isolate preparations of partially degraded fibrinogen molecules which could then be characterized for C00H-terminal Aα chain heterogeneity and crosslinking capacity to obtain a more precise localization of residues involved in early crosslinking. In order to assess the relative contribution of sites within the COOH-terminal region that includes 1 potential GLN at Aα #565 and 4 potential LYS at Aα #556, 562, 580 and 585, fibrinogen molecules that were missing these residues were isolated from preparations of purified fibrinogen using F-102-Sepharose immuno-affinity adsorption. Characterization of the partially degraded material (i.e., non-binding) by Western Blotting using F-102 and F-105 confirmed the presence of Aα remnants (29K-66K) that shared the structure Aα #1-276 but differed in the extent to which regions between ∼#276 and ∼#559 were preserved. When the crosslinking capacity of these partially degraded molecules was examined, using Western Blotting to monitor the appearance of crosslinked a chains during in vitro clotting, effective formation of early crosslinked species ( ∼100K, ∼ 200K) as well as eventual accumulation of a polymers, could be demonstrated. These findings indicate that at least one early a chain crosslink involves activity on the part of LYS and GLN residues located proximal to Aα #540. While these data do not rule out the existence of additional crosslinking sites situated more distally (i.e., within Aα #540-584), they do indicate that degraded fibrinogen molecules, circulating under pathophysiologic conditions, may undergo adequate fibrin stabilization despite a loss of at least 70 COOH-terminal Aα chain residues.
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Berndt, M. C., X. Du, L. Beutler, W. J. Booth et P. A. Castaldi. « LOCALIZATION OF FUNCTIONAL DOMAINS ON HUMAN PLATELET GP Ib-IX COMPLEX BY EPITOPE ANALYSIS WITH MONOCLONAL ANTIBODIES ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642923.
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There is now considerable evidence that glycoprotein (GP) Ib plays an important functional role in the von Willebrand factor (vWF)-dependent adhesion of platelets to exposed vascular subendothelium and in the a-thrombin activation of platelets, and that GP IX is important for quinine/quinidine drug-dependent antibody platelet recognition. GP Ib (Mr = 170 KD) consists of two disulfide-linked subunits, Iba (Mr = 135 KD) and Ibβ (Mr = 25 KD), and exists as a heterodimer complex with GP IX (Mr = 22 KD). In this study we have used a panel of 10 antiGP Ib-IX complex monoclonal antibodies to define the functional domains on this complex. Immunoprecipitation of trypsin-treated GP Ib-IX complex revealed that the monoclonal antibodies mapped into three distinct groups: FMC 25, AK 1 and SZ 1, epitopes on the membrane-associated fragment (GP IX and an ≃25 KD remnant of the α-chain disulfide linked to the β-chain); AK 3 and WM 23, epitopes on the central macroglycopeptide core (90 KD); AN 51, SZ 2, AK 2, AP 1 and HIP 1, epitopes on peptide tail (45 KD). Crossblocking studies indicated that with the exception of AK 1 and SZ 1, the monoclonal antibodies were directed against distinct epitopes. All five monoclonal antibodies directed against the peptide tail region blocked ristocetin-dependent vWF-platelet interaction whereas the other five monoclonal antibodies were without effect, indicating that the 45 KD peptide tail region at the plasma end of the α-chain of GP Ib contained the vWF binding domain. Similarly, only the three monoclonal antibodies directed against the membrane-associated region interfered with drug-dependent antibody-platelet interaction.By western blot analysis, α-thrombin bound to the 45 KD peptide tail region. However, only AP 1 interfered significantly with the α-thrombin-dependent aggregation of platelets. This panel of epitope-defined monoclonal antibodies should be of value in further defining the structure-function relationships of this important membrane complex.
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Естемесов, З. А., Б. К. Сарсенбаев, Г. О. Қаршыга, Н. Б. Сарсенбаев et А. М. Шакей. « MAIN CHARACTERISTICS OF GRANULATED PHOSPHORUS SLAG (GPS) USED FOR BINDING MATERIALS MANUFACTURING ». Dans «АКТУАЛЬНЫЕ ВОПРОСЫ СОВРЕМЕННОЙ НАУКИ : ТЕОРИЯ, ТЕХНОЛОГИЯ, МЕТОДОЛОГИЯ И ПРАКТИКА». Международная научно-практическая онлайн-конференция, приуроченная к 60-ти летию член-корреспондента Академии наук ЧР, доктора технических наук, профессора Сайд-Альви Юсуповича Муртазаева. Crossref, 2021. http://dx.doi.org/10.34708/gstou.conf..2021.60.48.037.
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Обзорный анализ теоретических и экспериментальных известных работ показал, что ГФШ, полученный при охлаждении водой расплава с температурой 1450°С, является пористым материалом со средней плотностью 1200 кг/м3. Состоит он из трех фаз:стекло в количестве 90 - 95 %, минералы (достигает 10 %) и вредные газы (0,3 - 4 %). Благодаря повышенной гидравлической активности - ГФШ может быть использован в качестве минеральной добавки для портландцемента, одного из компонентов для шлакопортландцемента и основного компонента для шлакощелочных вяжущих с марочностью М500 и М1000 соответственно. Одновременно существуют нормативные документы, разрешающие получать вяжущие материалы без очистки и неразрешающие, если ГФШ не очищено от вредных газов. Анализ показывает необходимость применения ГФШ только в очищенном виде. The review analysis of theoretical and experimental known works showed that GPS obtained by water cooling of the melt with temperature 1450°C is a porous material with average density of 1200 kg/m3. It consists of three phases: glass in quantity 90 - 95 %, minerals (reaches 10 %) and harmful gases (0,3 - 4 %). Thanks to the increased hydraulic activity - GPS can be used as a mineral additive for Portland cement, one of the components for Portland cement slag and the basic component for slag-alkali binders with the stamps of M500 and M1000 respectively. At the same time there are normative documents allowing to receive binders without purification and unauthorized, if GPS is not purified from harmful gases. The analysis shows the necessity to use GPS only in purified form.
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Pantović, Bojana V., Nevena Lj Stevanović, Tina P. Andrejević, Darko P. Ašanin, Miloš I. Djuran et Biljana Đ. Glišić. « DNA/BSA binding study of mononuclear gold(III) complexes with clinically used azoles ». Dans 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.399p.
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In the present study, we investigated the interactions of three mononuclear gold(III) complexes of the general formula [AuCl3(azole)], azole is clotrimazole (ctz, Au1), tioconazole (tcz, Au2) and voriconazole (vcz, Au3) with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission spectroscopy. A significant decrease in fluorescence intensity of BSA in the presence of the complexes and high values of the calculated binding constants have indicated their good binding affinity toward this model protein. Besides that, the investigated Au1-3 complexes have shown a significant binding affinity toward ct-DNA-EthBr system (EthBr is ethidium bromide, which behaves as an intercalator between DNA base pairs). The best binding affinity towards the studied biomolecules has been shown by Au3 complex, which contains a monodentately coordinated voriconazole. The findings from this study could be important for the further development of metal-based antimicrobials, as well as anticancer agents, with favourable activity and therapeutic profile in respect to the parent organic ligands.
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Sutton, Mauri, George Geoghegan, Kenneth Schopen, Kathleen Kingma, Steve Castro, Kyle Wesley, Jack Yahl et Frank Soto. « Non-Chemical Solder Bump Removal Technique for Repackaging Flip Chip ICs ». Dans ISTFA 2012. ASM International, 2012. http://dx.doi.org/10.31399/asm.cp.istfa2012p0485.
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Abstract In this paper we will discuss an empirically discovered technique to remove residual solder bumps or remnants using reflow and wicking to a gold plated surface rather than mechanical or chemical means. Extraction of flip chip ICs, for the purpose of repackaging, can leave bond pads in inconsistent and undesirable conditions such as 1) retaining remnants of the solder bumps or 2) damaged or eliminated pad metal caused by acid or mechanical means used to separate the IC from the board. These conditions hinder subsequent wire bonding and probe card use. Though other techniques have been found to be suitable at times, the technique described in this paper consistently leaves the bonding area in an acceptable, more predictable condition, as the bulk of the solder bump material is removed. This lends to a higher wire bonding success rate.
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Samkoe, Kimberley S., Kristian Sexton, Kenneth Tichauer, Scott C. Davis, Julia A. O'Hara, Tayyaba Hasan et Brian W. Pogue. « Determination of blood plasma fluorescence extinction coefficients for dyes used in three-compartment binding model ». Dans SPIE BiOS, sous la direction de David H. Kessel et Tayyaba Hasan. SPIE, 2011. http://dx.doi.org/10.1117/12.875408.
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Berkner, J. A., G. Mitra et J. W. Bloom. « MONOCLONAL ANTIBODY BINDING TO FACTOR VIII:C ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644063.
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The interactions of monoclonal antibodies with highly purified Factor VIII:c have been studied utilizing the ELISA technique. ELISA plates were coated with Factor VIII:c, protein A purified monoclonal IgG was then added and bound antibody detected with peroxidase labeled antimouse IgG. A Scatchard-Sips plot approach to data analysis was used to calculate binding constants. The binding constants for four antibodies designated BD10, AD7, C7F7 and 39MH8 were as follows: BD10, KO = 7.1 x 108 M-1, n = 1.1 (moles antibody/moles ligand); AD7, KO = 3.1 x 108 M-1, n = 2.7; C7F7, KO = 3.6 x 1011M-1, n = 0.03; 39MH8, K = 6.0 x 1011 M-1, n = 0.03. The binding constants for C7F7 to the purified carboxy-terminal (residues 1649-2332) 80 kD functional region of the Factor VIII:c molecule were also determined: KO = 1.0 x 1011 M-1, n = 0.55. On the basis of these results the following conclusions can be drawn: 1) the antibodies can be divided into two groups: high affinity (suitable for use in immunopurification), C7F7 and 39MH8; low affinity: BD10 and AD7; 2) the antibodies in the low affinity group have valance values two orders of magnitude higher than the high affinity antibodies, C7F7 and 39MH8. The difference might be explained by the high affinity antibody epitopes on the immobilized Factor VIII:c being less exposed to the solution; 3) C7F7 binding to the 80 kD polypeptide, compared to the whole Factor VIII:c molecule, gave virtually identical Kc values, but dramatically different valance values. This suggests that the C7F7 epitope is more accessible on the 80 kD polypeptide.
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Björk, I., S. T. Olson et J. D. Shore. « BINDING OF HEPARIN TO H-KININOGEN ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642853.
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The binding of heparin to kininogen was analyzed by competition of kininogen with anti thrombin for high-affinity heparin. Residual heparin binding to anti thrombin was quantified by the accelerating effect on the anti thrombin-thrombin reaction. The rate of the latter reaction was monitored by displacement of the fluorescent probe, p-aminobenzamidine, from the enzyme. A linear dependence of the observed pseudo-first-order rate constant (kobs) for the heparin-accelerated anti thrombin-thrombin reaction on heparin concentration was achieved by use of catalytic amounts (≤30 nM) of heparin, a 20-fold ratio of anti thrombin to thrombin and thrombin concentrations (0.25 μM) much below the apparent of heparin for thrombin at the high (1 mM) p-aminobenzamidine concentration used. The two-chain form of H-kininogen minimally affected the heparin-accelerated rate of the anti-thrombin-thrombin reaction at pH 7.4 in the absence of metal ions. However, at saturating concentrations of Zn2+ (10 μM), kobs was reduced to 50% at ˜15 nM kininogen and to that of the uncatalyzed reaction at ≥˜0.25 μM. Conversely, at saturating kininogen, a 50% decrease of kobs was observed at ˜0.6 μM Zn2+, i.e. in the plasma concentration range. Other metal ions were effective in the order: Zn2+˜Ni2+>Cu2+>Co2+˜Cd2+. Single-chain H-kininogen and H-kininogen light chain reduced the heparin enhancement in the presence of Zn2+ to the same extent as the two-chain form, whereas L-kininogen had no effect. In the absence of metal ions, the binding of heparin to two-chain H-kini-nogen increased with decreasing pH below 7.4 in a manner consistent with involvement of protonated histidine residues. Thus, heparin presumably binds to the histidine-rich region of the light chain portion of H-kininogen. The elution of two-chain H-kininogen from immobilized dextran sulfate at pH 7.4 was shifted to higher salt concentrations in the presence of 10 μM Zn2+, indicating that metal ions may also enhance H-kininogen binding to surfaces relevant to contact activation reactions. The sensitivity of H-kininogen-surface interactions to divalent metal ions and pH suggest regulation of the interactions by these factors. Like histidine-rich glycoprotein, H-kininogen may also compete with anti thrombin for heparin during heparin therapy.
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Shah, Jami J., et Viren Pherwani. « Dynamic Binding of Features and Solid Modelers ». Dans ASME 1993 Design Technical Conferences. American Society of Mechanical Engineers, 1993. http://dx.doi.org/10.1115/detc1993-0401.
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Abstract The work described in this paper investigates the feasibility of standardizing communications between geometric modeling core systems and generic feature-based applications. Since geometric modelers differ in the functionality they provide and feature applications vary in the level of geometric operations they can support internally, a multi-layered communication architecture is proposed. The methodology is analogous to the X-Window standard for graphics. At the lowest level is a library of functions named Geo-lib, which are translated into geometric modeler specific commands. If there was to be a future dynamic interfacing standard, such as STEP-SDAI, these specific calls could be replaced by standard calls, analogous to Geo-Protocol. At the next layer is a library, called Geo-widgets, which are written entirely using Geo-lib functions. At the highest level Geo-Tools, functions used commonly by generic applications. Feature applications can choose to use the library at any level, as necessary. This multi-layered geometric toolkit creates a seamless object oriented bond between the feature application and the geometric modeling core, in such a way that either one could be replaced without requiring any changes to the other.
Rapports d'organisations sur le sujet "Binding, remnants used in"
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Sebesta, F., J. John, A. Motl et K. Stamberg. Evaluation of polyacrylonitrile (PAN) as a binding polymer for absorbers used to treat liquid radioactive wastes. Office of Scientific and Technical Information (OSTI), novembre 1995. http://dx.doi.org/10.2172/168591.
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Sebesta, F., J. John et A. Motl. Phase 2 report on the evaluation of polyacrylonitrile (PAN) as a binding polymer for absorbers used to treat liquid radioactive wastes. Office of Scientific and Technical Information (OSTI), mai 1996. http://dx.doi.org/10.2172/231361.
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Abdul Rahim, Nor Azura, Noor Syazril Jafri et Muna'amirah Mohamad. DEVELOPMENT OF OIL ABSORBENT MAT FROM WASTE NBR GLOVES. Penerbit Universiti Malaysia Perlis, 2023. http://dx.doi.org/10.58915/techrpt2023.003.
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Waste NBR rubber gloves have become an environmental burden because the chemically cross-linked NBR gloves can’t be recycled. In this particular research, an initiative was made to turn the NBR waste glove into an oil absorbent mat by using the amount of chemical cross-linking inside the NBR waste glove triggered by solvents and with the help of epoxy resin as a binding agent. The absorption test used two types of oil: cooking oil and engine oil. To justify the relationship between the developed oil absorbent mat and its absorption behaviour, various tests, namely the Fourier transform infrared radiation (FTIR), scanning electron microscopy (SEM), and absorbency test, were conducted. Epoxy resin was used as a binding agent in the production of oil-absorbing mats made from waste NBR gloves. The NBR gloves mixed with binding agents of epoxy resin mats have proven to be an effective medium for oil absorbance purposes.
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Li, Yuan, Benjamin Metcalf, Sopio Chochua, Zhongya Li, Robert Gertz, Hollis Walker, Paulina Hawkins, Theresa Tran, Lesley McGee et Bernard W. Beall. Validation of β-lactam minimum inhibitory concentration predictions for pneumococcal isolates with newly encountered penicillin binding protein (PBP) sequences [Supporting data]. Centers for Disease Control and Prevention (U.S.), novembre 2017. http://dx.doi.org/10.15620/cdc/147467.
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The datafiles, R scripts, MIC tables, and other files were used to evaluate the prediction performance of a penicillin-binding protein (PBP) typing system and two methods (Random Forest (RF) and Mode MIC (MM) previously developed by this research team. This data and these files support the finding of the paper "Validation of β-lactam minimum inhibitory concentration predictions for pneumococcal isolates with newly encountered penicillin binding protein (PBP) sequences" at https://doi.org/10.1186%2Fs12864-017-4017-7 or at https://stacks.cdc.gov/view/cdc/47684.
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Morrison, Mark, et Joshuah Miron. Molecular-Based Analysis of Cellulose Binding Proteins Involved with Adherence to Cellulose by Ruminococcus albus. United States Department of Agriculture, novembre 2000. http://dx.doi.org/10.32747/2000.7695844.bard.
Texte intégralRésumé :
At the beginning of this project, it was clear that R. albus adhered tightly to cellulose and its efficient degradation of this polysaccharide was dependent on micromolar concentrations of phenylacetic acid (PAA) and phenylpropionic acid (PPA). The objectives for our research were: i) to identify how many different kinds of cellulose binding proteins are produced by Ruminococcus albus; ii) to isolate and clone the genes encoding some of these proteins from the same bacterium; iii) to determine where these various proteins were located and; iv) quantify the relative importance of these proteins in affecting the rate and extent to which the bacterium becomes attached to cellulose. BARD support has facilitated a number of breakthroughs relevant to our fundamental understanding of the adhesion process. First, R. albus possesses multiple mechanisms for adhesion to cellulose. The P.I.'s laboratory has discovered a novel cellulose-binding protein (CbpC) that belongs to the Pil-protein family, and in particular, the type 4 fimbrial proteins. We have also obtained genetic and biochemical evidence demonstrating that, in addition to CbpC-mediated adhesion, R. albus also produces a cellulosome-like complex for adhesion. These breakthroughs resulted from the isolation (in Israel and the US) of spontaneously arising mutants of R. albus strains SY3 and 8, which were completely or partially defective in adhesion to cellulose, respectively. While the SY3 mutant strain was incapable of growth with cellulose as the sole carbon source, the strain 8 mutants showed varying abilities to degrade and grow with cellulose. Biochemical and gene cloning experiments have been used in Israel and the US, respectively, to identify what are believed to be key components of a cellulosome. This combination of cellulose adhesion mechanisms has not been identified previously in any bacterium. Second, differential display, reverse transcription polymerase chain reaction (DD RT-PCR) has been developed for use with R. albus. A major limitation to cellulose research has been the intractability of cellulolytic bacteria to genetic manipulation by techniques such as transposon mutagenesis and gene displacement. The P.I.'s successfully developed DD RT- PCR, which expanded the scope of our research beyond the original objectives of the project, and a subset of the transcripts conditionally expressed in response to PAA and PPA have been identified and characterized. Third, proteins immunochemically related to the CbpC protein of R. albus 8 are present in other R. albus strains and F. intestinalis, Western immunoblots have been used to examine additional strains of R. albus, as well as other cellulolytic bacteria of ruminant origin, for production of proteins immunochemically related to the CbpC protein. The results of these experiments showed that R. albus strains SY3, 7 and B199 all possess a protein of ~25 kDa which cross-reacts with polyclonal anti-CbpC antiserum. Several strains of Butyrivibrio fibrisolvens, Ruminococcus flavefaciens strains C- 94 and FD-1, and Fibrobacter succinogenes S85 produced no proteins that cross-react with the same antiserum. Surprisingly though, F. intestinalis strain DR7 does possess a protein(s) of relatively large molecular mass (~200 kDa) that was strongly cross-reactive with the anti- CbpC antiserum. Scientifically, our studies have helped expand the scope of our fundamental understanding of adhesion mechanisms in cellulose-degrading bacteria, and validated the use of RNA-based techniques to examine physiological responses in bacteria that are nor amenable to genetic manipulations. Because efficient fiber hydrolysis by many anaerobic bacteria requires both tight adhesion to substrate and a stable cellulosome, we believe our findings are also the first step in providing the resources needed to achieve our long-term goal of increasing fiber digestibility in animals.
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Fernando, P. U. Ashvin Iresh, Gilbert Kosgei, Matthew Glasscott, Garrett George, Erik Alberts et Lee Moores. Boronic acid functionalized ferrocene derivatives towards fluoride sensing. Engineer Research and Development Center (U.S.), juillet 2022. http://dx.doi.org/10.21079/11681/44762.
Texte intégralRésumé :
In this technical report (TR), a robust, readily synthesized molecule with a ferrocene core appended with one or two boronic acid moieties was designed, synthesized, and used toward F- (free fluoride) detection. Through Lewis acid-base interactions, the boronic acid derivatives are capable of binding with F- in an aqueous solution via ligand exchange reaction and is specific to fluoride ion. Fluoride binding to ferrocene causes significant changes in fluorescence or electrochemical responses that can be monitored with field-portable instrumentation at concentrations below the WHO recommended limit. The F- binding interaction was further monitored via proton nuclear magnetic resonance spectroscopy (1H-NMR). In addition, fluorescent spectroscopy of the boronic acid moiety and electrochemical monitoring of the ferrocene moiety will allow detection and estimation of F- concentration precisely in a solution matrix. The current work shows lower detection limit (LOD) of ~15 μM (285 μg/L) which is below the WHO standards. Preliminary computational calculations showed the boronic acid moieties attached to the ferrocene core interacted with the fluoride ion. Also, the ionization diagrams indicate the amides and the boronic acid groups can be ionized forming strong ionic interactions with fluoride ions in addition to hydrogen bonding interactions.
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Chamovitz, Daniel A., et Zhenbiao Yang. Chemical Genetics of the COP9 Signalosome : Identification of Novel Regulators of Plant Development. United States Department of Agriculture, janvier 2011. http://dx.doi.org/10.32747/2011.7699844.bard.
Texte intégralRésumé :
This was an exploratory one-year study to identify chemical regulators of the COP9 signalosome. Chemical Genetics uses small molecules to modify or disrupt the function of specific genes/proteins. This is in contrast to classical genetics, in which mutations disrupt the function of genes. The underlying concept is that the functions of most proteins can be altered by the binding of a chemical, which can be found by screening large libraries for compounds that specifically affect a biological, molecular or biochemical process. In addition to screens for chemicals which inhibit specific biological processes, chemical genetics can also be employed to find inhibitors of specific protein-protein interactions. Small molecules altering protein-protein interactions are valuable tools in probing protein-protein interactions. In this project, we aimed to identify chemicals that disrupt the COP9 signalosome. The CSN is an evolutionarily conserved eight-subunit protein complex whose most studied role is regulation of E3 ubiquitinligase activity. Mutants in subunits of the CSN undergo photomorphogenesis in darkness and accumulate high levels of pigments in both dark- and light-grown seedlings, and are defective in a wide range of important developmental and environmental-response pathways. Our working hypothesis was that specific molecules will interact with the CSN7 protein such that binding to its various interacting proteins will be inhibited. Such a molecule would inhibit either CSN assembly, or binding of CSN-interacting proteins, and thus specifically inhibit CSN function. We used an advanced chemical genetic screen for small-molecule-inhibitors of CSN7 protein-protein interactions. In our pilot study, following the screening of ~1200 unique compounds, we isolated four chemicals which reproducibly interfere with CSN7 binding to either CSN8 or CSN6.
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Yermiyahu, Uri, Thomas Kinraide et Uri Mingelgrin. Role of Binding to the Root Surface and Electrostatic Attraction in the Uptake of Heavy Metal by Plants. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7586482.bard.
Texte intégralRésumé :
The principal accomplishment of the research supported by BARD was progress toward a comprehensive view of cell-surface electrical effects (both in cell walls [CWs] and at plasma membrane [PM] surfaces) upon ion uptake, intoxication, and amelioration. The research confirmed that electrostatic models (e.g., Gouy-Chapman-Stern [G-C-S]), with parameter values contributed by us, successfully predict ion behavior at cell surfaces. Specific research objectives 1. To characterize the sorption of selected heavy metals (Cu, Zn, Pb, Cd) to the root PM in the presence of other cations and organic ligands (citric and humic acids). 2. To compute the parameters of a G-C-S model for heavy-metal sorption to the root PM. 3. To characterize the accumulation of selected heavy metals in various plant parts. 4. To determine whether model-computed ion binding or ion activities at root PM surfaces predict heavy-metal accumulation in whole roots, root tips, or plant shoots. 5. To determine whether measured ion binding by protoplast-free roots (i.e., root CWs) predicts heavy-metal accumulation in whole roots, root tips, or plant shoots. 6. To correlate growth inhibition, and other toxic responses, with the measured and computed factors mentioned above. 7. To determine whether genotypic differences in heavy-metal accumulation and toxic responses correlate with genotypic differences in parameters of the G-C-S model. Of the original objectives, all except for objective 7 were met. Work performed to meet the other objectives, and necessitated on the basis of experimental findings, took the time that would have been required to meet objective 7. In addition, work with Pb was unsuccessful due to experimental complications and work on Cd is still in progress. On the other hand, the uptake and toxicity of the anion, selenate was characterized with respect to electrostatic effects and the influences of metal cations. In addition, the project included more theoretical work, supported by experimentation, than was originally planned. This included transmembrane ion fluxes considered in terms of PM-surface electrical potentials and the influence of CWs upon ion concentrations at PM surfaces. A important feature of the biogeochemistry of trace elements in the rhizosphere is the interaction between plant-root surfaces and the ions present in the soil solution. The ions, especially the cations, of the soil solution may be accumulated in the aqueous phases of cell surfaces external to the PMs, sometimes referred to as the "water free space" and the "Donnan free space". In addition, ions may bind to the CW components or to the PM surface with variable binding strength. Accumulation at the cell surface often leads to accumulation in other plant parts with implications for the safety and quality of foods. A G-C-S model for PMs and a Donnan-plus-binding model for CWs were used successfully to compute electrical potentials, ion binding, and ion concentration at root-cell surfaces. With these electrical potentials, corresponding values for ion activities may be computed that are at least proportional to actual values also. The computed cell-surface ion activities predict and explain ion uptake, intoxication, and amelioration of intoxication much more accurately than ion activities in the bulk-phase rooting medium.
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Fluhr, Robert, et Maor Bar-Peled. Novel Lectin Controls Wound-responses in Arabidopsis. United States Department of Agriculture, janvier 2012. http://dx.doi.org/10.32747/2012.7697123.bard.
Texte intégralRésumé :
Innate immune responses in animals and plants involve receptors that recognize microbe-associated molecules. In plants, one set of this defense system is characterized by large families of TIR–nucleotide binding site–leucine-rich repeat (TIR-NBS-LRR) resistance genes. The direct interaction between plant proteins harboring the TIR domain with proteins that transmit and facilitate a signaling pathway has yet to be shown. The Arabidopsis genome encodes TIR-domain containing genes that lack NBS and LRR whose functions are unknown. Here we investigated the functional role of such protein, TLW1 (TIR LECTIN WOUNDRESPONSIVE1). The TLW1 gene encodes a protein with two domains: a TIR domain linked to a lectin-containing domain. Our specific aim in this proposal was to examine the ramifications of the TL1-glycan interaction by; A) The functional characterization of TL1 activity in the context of plant wound response and B) Examine the hypothesis that wounding induced specific polysaccharides and examine them as candidates for TL-1 interactive glycan compounds. The Weizmann group showed TLW1 transcripts are rapidly induced by wounding in a JA-independent pathway and T-DNA-tagged tlw1 mutants that lack TLW1 transcripts, fail to initiate the full systemic wound response. Transcriptome methodology analysis was set up and transcriptome analyses indicates a two-fold reduced level of JA-responsive but not JA-independent transcripts. The TIR domain of TLW1 was found to interact directly with the KAT2/PED1 gene product responsible for the final b-oxidation steps in peroxisomal-basedJA biosynthesis. To identify potential binding target(s) of TL1 in plant wound response, the CCRC group first expressed recombinant TL1 in bacterial cells and optimized conditions for the protein expression. TL1 was most highly expressed in ArcticExpress cell line. Different types of extraction buffers and extraction methods were used to prepare plant extracts for TL1 binding assay. Optimized condition for glycan labeling was determined, and 2-aminobenzamide was used to label plant extracts. Sensitivity of MALDI and LC-MS using standard glycans. THAP (2,4,6- Trihydroxyacetophenone) showed minimal background peaks at positive mode of MALDI, however, it was insensitive with a minimum detection level of 100 ng. Using LC-MS, sensitivity was highly increased enough to detect 30 pmol concentration. However, patterns of total glycans displayed no significant difference between different extraction conditions when samples were separated with Dionex ICS-2000 ion chromatography system. Transgenic plants over-expressing lectin domains were generated to obtain active lectin domain in plant cells. Insertion of the overexpression construct into the plant genome was confirmed by antibiotic selection and genomic DNA PCR. However, RT-PCR analysis was not able to detect increased level of the transcripts. Binding ability of azelaic acid to recombinant TL1. Azelaic acid was detected in GST-TL1 elution fraction, however, DHB matrix has the same mass in background signals, which needs to be further tested on other matrices. The major findings showed the importance of TLW1 in regulating wound response. The findings demonstrate completely novel and unexpected TIR domain interactions and reveal a control nexus and mechanism that contributes to the propagation of wound responses in Arabidopsis. The implications are to our understanding of the function of TIR domains and to the notion that early molecular events occur systemically within minutes of a plant sustaining a wound. A WEB site (http://genome.weizmann.ac.il/hormonometer/) was set up that enables scientists to interact with a collated plant hormone database.
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Gershoni, Jonathan M., David E. Swayne, Tal Pupko, Shimon Perk, Alexander Panshin, Avishai Lublin et Natalia Golander. Discovery and reconstitution of cross-reactive vaccine targets for H5 and H9 avian influenza. United States Department of Agriculture, janvier 2015. http://dx.doi.org/10.32747/2015.7699854.bard.
Texte intégralRésumé :
Research objectives: Identification of highly conserved B-cell epitopes common to either H5 or H9 subtypes of AI Reconstruction of conserved epitopes from (1) as recombinantimmunogens, and testing their suitability to be used as universal vaccine components by measuring their binding to Influenza vaccinated sera of birds Vaccination of chickens with reconstituted epitopes and evaluation of successful vaccination, clinical protection and viral replication Development of a platform to investigate the dynamics of immune response towards infection or an epitope based vaccine Estimate our ability to focus the immune response towards an epitope-based vaccine using the tool we have developed in (D) Summary: This study is a multi-disciplinary study of four-way collaboration; The SERPL, USDA, Kimron-Israel, and two groups at TAU with the purpose of evaluating the production and implementation of epitope based vaccines against avian influenza (AI). Systematic analysis of the influenza viral spike led to the production of a highly conserved epitope situated at the hinge of the HA antigen designated “cluster 300” (c300). This epitope consists of a total of 31 residues and was initially expressed as a fusion protein of the Protein 8 major protein of the bacteriophagefd. Two versions of the c300 were produced to correspond to the H5 and H9 antigens respectively as well as scrambled versions that were identical with regard to amino acid composition yet with varied linear sequence (these served as negative controls). The recombinantimmunogens were produced first as phage fusions and then subsequently as fusions with maltose binding protein (MBP) or glutathioneS-transferase (GST). The latter were used to immunize and boost chickens at SERPL and Kimron. Furthermore, vaccinated and control chickens were challenged with concordant influenza strains at Kimron and SEPRL. Polyclonal sera were obtained for further analyses at TAU and computational bioinformatics analyses in collaboration with Prof. Pupko. Moreover, the degree of protection afforded by the vaccination was determined. Unfortunately, no protection could be demonstrated. In parallel to the main theme of the study, the TAU team (Gershoni and Pupko) designed and developed a novel methodology for the systematic analysis of the antibody composition of polyclonal sera (Deep Panning) which is essential for the analyses of the humoral response towards vaccination and challenge. Deep Panning is currently being used to monitor the polyclonal sera derived from the vaccination studies conducted at the SEPRL and Kimron.