Articles de revues : « Binding media »

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Tumosa, Charles S., Dusan Stulik et Henry Florsheim. « Binding Media Identification ». Journal of the American Institute for Conservation 32, n^o 2 (1993) : 207. http://dx.doi.org/10.2307/3179710.

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Rella, R., A. Sturaro, G. Parvoli, D. Ferrara et U. Casellato. « Identification of Binding Media in Paintings ». Chromatographia 63, n^o 11-12 (19 mai 2006) : 629–31. http://dx.doi.org/10.1365/s10337-006-0803-0.

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Scardina, Paolo, et Marc Edwards. « Air Binding of Granular Media Filters ». Journal of Environmental Engineering 130, n^o 10 (octobre 2004) : 1126–38. http://dx.doi.org/10.1061/(asce)0733-9372(2004)130:10(1126).

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Stulik, Dusan, et Henry Florsheim. « Binding Media Identification in Painted Ethnographic Objects ». Journal of the American Institute for Conservation 31, n^o 3 (1992) : 275. http://dx.doi.org/10.2307/3179724.

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Stulik, Dusan, et Henry Florsheim. « Binding Media Identification in Painted Ethnographic Objects ». Journal of the American Institute for Conservation 31, n^o 3 (janvier 1992) : 275–88. http://dx.doi.org/10.1179/019713692806066565.

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Simard, Marc, Lesley A. Hill, Caroline M. Underhill, Bernd O. Keller, Ivan Villanueva, Robert E. W. Hancock et Geoffrey L. Hammond. « Pseudomonas Aeruginosa Elastase Disrupts the Cortisol-Binding Activity of Corticosteroid-Binding Globulin ». Endocrinology 155, n^o 8 (1 août 2014) : 2900–2908. http://dx.doi.org/10.1210/en.2014-1055.

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The serine protease inhibitor (SERPIN) family member corticosteroid-binding globulin (CBG) is the main carrier of glucocorticoids in plasma. Human CBG mediates the targeted release of cortisol at sites of inflammation through cleavage of its reactive center loop (RCL) by neutrophil elastase. The RCLs of SERPIN family members are targeted by diverse endogenous and exogenous proteases, including several bacterial proteases. We tested different bacteria for their ability to secrete proteases that disrupt CBG cortisol-binding activity, and characterized the responsible protease and site of CBG cleavage. Serum CBG integrity was assessed by Western blotting and cortisol-binding capacity assay. Effects of time, pH, temperature, and protease inhibitors were tested. Proteolytically active proteins from bacterial media were purified by fast protein liquid chromatography, and the active protease and CBG cleavage sites were identified by mass spectrometry. Among the bacteria tested, medium from Pseudomonas aeruginosa actively disrupted the cortisol-binding activity of CBG. This proteolytic activity was inhibited by zinc chelators and occurred most efficiently at pH 7 and elevated physiological temperature (ie, 41°C). Mass spectrometric analysis of a semi-purified fraction of P. aeruginosa media identified the virulence factor LasB as the responsible protease, and this was confirmed by assaying media from LasB-deficient P. aeruginosa. This metalloprotease cleaves the CBG RCL at a major site, distinct from that targeted by neutrophil elastase. Our results suggest that humoral responses to P. aeruginosa infection are influenced by this pathogen's ability to secrete a protease that promotes the release of the anti-inflammatory steroid, cortisol, from its plasma transport protein.

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Pinto, Sílvia, et Moisés de Lemos Martins. « Binding logics in art ». Comunicação e Sociedade 31 (29 juin 2017) : 271–86. http://dx.doi.org/10.17231/comsoc.31(2017).2617.

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The work of art produced in its origins was only much later recognized as such. Similarly, the artistic function of current art objects in the future may become accidental again. In fact, at no time has art ever answered exclusively to aesthetic demands. From these assumptions by Walter Benjamin (1936-1939/1992), we will attempt to apply to art the concepts of “binding” (original of ethology) and of “linking networks” (used in neuroscience) to explain distinctive aspects of image metaphysics, shared by art and religion. From a historical perspective, we will attempt to show the evolution of art in three main binding logics: art as a magical activity (also in relation to index logic); art as mimesis; and art as language. The image, or rather the multiple realities we call “image”, takes each one of these links, in an exchanging or simultaneous way, since image is their heiress. Taking into account the present context of media images hypervisibility, we aim, with this study, to show the importance of art and its mythical-religious ascendance in what concerns media image redefinition.

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Sabharwal, Vishakha, Sanjay Ram, Marisol Figueira, In Ho Park et Stephen I. Pelton. « Role of Complement in Host Defense against Pneumococcal Otitis Media ». Infection and Immunity 77, n^o 3 (12 janvier 2009) : 1121–27. http://dx.doi.org/10.1128/iai.01148-08.

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ABSTRACT Strategies to limit complement deposition on Streptococcus pneumoniae are established as virulence features for invasive disease, but their role in respiratory tract infection requires further analysis. We evaluated complement C3 protein deposition on discordant S. pneumoniae isolates of the same serotype (6A) and their capacity to cause nasopharyngeal (NP) colonization and experimental otitis media (EOM) in an animal model. We compared C3 binding to five 6A isolates from asymptomatic NP carriers with five 6A strains that caused invasive disease, and we observed less C3 (∼10-fold less fluorescence) binding to invasive isolates. We selected two high-level C3-binding carriage and two low-level C3-binding invasive 6A isolates for further study. In the EOM model, 11/12 (92%) ears challenged with a low-level C3-binding 6A strain became infected. Only 2/8 (25%) ears challenged with the discordant high-level C3-binding 6A isolate developed disease (P = 0.005). Results with the second discordant 6A isolate pair were comparable. Cobra venom factor (CoVF) treatment, which depletes C3 and consumes complement, restored virulence of the high-level C3-binding strain; 8/8 (100%) ears in CoVF-treated animals developed EOM compared to only 25% of ears in naïve animals (P = 0.007). These studies demonstrate the critical role for complement evasion in pneumococcal EOM. Colonization with carriage isolates that bound high levels of C3 caused EOM in fewer animals compared to low-level C3-binding invasive strains. Thus, limiting C3 deposition on the surface of S. pneumoniae correlates with increased incidence of EOM following NP colonization and barotrauma in the animal model.

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Hermans, Joen J., Katrien Keune, Annelies van Loon, Robert W. Corkery et Piet D. Iedema. « Ionomer-like structure in mature oil paint binding media ». RSC Advances 6, n^o 96 (2016) : 93363–69. http://dx.doi.org/10.1039/c6ra18267d.

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Budi, Sulistyaning, Annisa Fitri Nurlaila, Icaq Dwi Prasetyo, Indah Nur Rahmadhani, Javier Sebastian et Iqmal Tahir. « Molecular Docking of Gallic Acid and Its Derivatives as the Potential nNOS Inhibitors ». Jurnal Kimia Sains dan Aplikasi 25, n^o 6 (17 mai 2022) : 197–204. http://dx.doi.org/10.14710/jksa.25.6.197-204.

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The global prevalence of anxiety and depression rates have increased by 25% due to the impact of the COVID-19 pandemic. Depression can occur due to an increase in NO produced by the nNOS enzyme. Gallic acid and its derivatives can be obtained from nature and have various biological activities. This study aimed to determine the potential of gallic acid and its derivatives as nNOS inhibitors using the molecular docking method with parameters of binding energy values, RMSD values, and specific binding to amino acid residues. The results showed that gallic acid, 4-O-methyl gallic acid, and epigallocatechin gallate had bond energies of −1.87; −2.36; and −0.12 kcal/mol, respectively. Compared to the standard ligand, which had binding energy of −2.84 kcal/mol, gallic acid 4-O-(6-galloyl glucoside) had binding energy of −4.12 kcal/mol. Based on these results, gallic acid 4-O-(6-galloyl glucoside) can potentially inhibit nNOS.

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Broschat, Timothy K. « Manganese Binding by Municipal Waste Composts Used as Potting Media ». Journal of Environmental Horticulture 9, n^o 2 (1 juin 1991) : 97–100. http://dx.doi.org/10.24266/0738-2898-9.2.97.

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Abstract Queen palms (Syagrus romanzoffiana (Chamisso) Glassman) grown in several types of sewage sludge compost media developed severe Mn deficiency symptoms. Seventy of the symptoms was correlated with DTPA-extractable Mn levels in the media and with leaf Mn content, but not with total media Mn. Compost media tied up over 70% of Mn added to samples within one hour, versus 62% or less for a pine bark, sedge peat, and sand medium. Analysis of autoclaved media samples suggested that some of the Mn tie up in garbage and yard trash composts is caused by microorganisms, but microorganisms had little effect on the binding potential of sludge and manure composts.

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Arias, C. A., et H. Brix. « Phosphorus removal in constructed wetlands : can suitable alternative media be identified ? » Water Science and Technology 51, n^o 9 (1 mai 2005) : 267–73. http://dx.doi.org/10.2166/wst.2005.0335.

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Removal of phosphorous in constructed wetlands is limited by the capacity of the media to adsorb, bind or precipitate the incoming P. To enhance P removal and the life span of constructed wetlands the approach might be to use natural sands rich in calcium or iron, to use an alternative ‘artificial’ medium with high P-binding capacity, or to establish external P-binding filters after the wetland. Our studies focused on the evaluation of calcium-rich materials potentially useful as P-binding media. The materials tested included calcite products, natural sands and seashells. Tests included assessment of physical and chemical properties of the materials, extractions in P-spiked water at different P concentrations to determine P-binding equilibrium isotherms, and column experiments. In addition, full-scale tests were performed with calcite in an external filter. The result showed that equilibrium isotherm is an indicator of the potential P-sorption capacity of the media, although the value is of limited application for the determination of the binding capacity in full-scale systems. The columns showed that the materials do bind phosphorus. However, the binding capacities are still insufficient for the establishment of external P-removal filter; the volumes of the filters would be too large to be of practical use.

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Fulcher, Kate, Neal Spencer, Julia Budka et Rebecca J. Stacey. « Polysaccharide Paint Binding Media at Two Pharaonic Settlements in Nubia ». Heritage 5, n^o 3 (2 août 2022) : 2028–40. http://dx.doi.org/10.3390/heritage5030106.

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Paints and plasters from two pharaonic settlement sites in Nubia (northern Sudan) were analysed to investigate the presence and origin of organic binding materials. The town of Sai was founded around the time of the pharaonic conquest of Kush (Upper Nubia) around 1500 BC, with Amara West created as a new centre for the pharaonic administration of the region around 1300 BC. Recent fieldwork at both sites yielded examples of paint palettes, including several from houses. These provide a different economic and social context to funerary contexts upon which most previous research has been conducted, making this study the first to report on binding media for vernacular architecture in the Nile Valley. It is also the first study of binding media from Nubia. Gas chromatography mass spectrometry (GC-MS) analysis of methanolysed and silylated paint and plaster samples revealed a range of monosaccharides present in eight of the seventeen samples from Amara West, and in six of the seven samples from Sai. Interpretation of the data was supported by field collection and study of locally available botanical gums. The results indicate that mixtures of gums were in use as a pigment binder at both sites during the mid- to late-second millennium BC. The possibility that some of these plant gums could have been imported from the Mediterranean is also posited.

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Thomas-Kerr, Joseph, Ian Burnett et Christian Ritz. « Format-Independent Rich Media Delivery Using the Bitstream Binding Language ». IEEE Transactions on Multimedia 10, n^o 3 (avril 2008) : 514–22. http://dx.doi.org/10.1109/tmm.2008.917337.

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Orlova, Darya Yu, Eva Bártová, Valeri P. Maltsev, Stanislav Kozubek et Andrei V. Chernyshev. « FRAP Analysis of Proteins Diffusion and Binding in Inhomogeneous Media ». Biophysical Journal 102, n^o 3 (janvier 2012) : 48a. http://dx.doi.org/10.1016/j.bpj.2011.11.294.

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White, Nicholas G., Sílvia Carvalho, Vítor Félix et Paul D. Beer. « Anion binding in aqueous media by a tetra-triazolium macrocycle ». Organic & ; Biomolecular Chemistry 10, n^o 34 (2012) : 6951. http://dx.doi.org/10.1039/c2ob25934f.

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Learner, Tom. « A review of synthetic binding media in twentieth‐century paints ». Conservator 24, n^o 1 (janvier 2000) : 96–103. http://dx.doi.org/10.1080/01410096.2000.9995156.

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MARIC, CHRISTINE, DAVID CASLEY, PETER J. HARRIS et DAINE ALCORN. « Angiotensin II Binding to Renomedullary Interstitial Cells Is Regulated by Osmolality ». Journal of the American Society of Nephrology 12, n^o 3 (mars 2001) : 450–55. http://dx.doi.org/10.1681/asn.v123450.

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Abstract. Angiotensin II (Ang II) AT1A receptors are localized to renomedullary interstitial cells (RMIC) in the inner stripe of the outer medulla but not in the inner medulla. Thus, there seems to be a correlation between decreases in AT1A receptor binding to RMIC and increases in interstitial osmolality, suggesting that osmolality is important in determining Ang II binding to RMIC. Cultured RMIC were incubated in media of differing osmolalities (330, 630, 930, and 1230 mOsm/kgH2O). 125I-[Sar1, Ile8] Ang II binding to AT1A receptors on RMIC grown in hyperosmolal media (930 mOsm/kgH2O) was reduced compared with isoosmolal (330 mOsm/kgH2O) media and was progressively reduced with further increases of osmolality. Similar studies were performed using bradykinin (BK) as a control peptide. Binding of the BK receptor ligand 125I-[HPP-Hoe 140] to B2 receptors was not affected by varying osmolality of the media. Reverse transcriptase-PCR demonstrated the presence of the mRNA expression for both AT1A and B2 receptors at each osmolality. The conclusion is that osmolality modulates Ang II binding to RMIC; in these cells, this phenomenon is restricted to Ang II as BK binding is not affected. Osmolality-induced changes in Ang II binding may modulate the actions of this peptide on RMIC and provide an important mechanism by which these cells modulate renal medullary function.

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Goriely, Anita R., Ann L. Baldwin et Timothy W. Secomb. « Transient diffusion of albumin in aortic walls : effects of binding to medial elastin layers ». American Journal of Physiology-Heart and Circulatory Physiology 292, n^o 5 (mai 2007) : H2195—H2201. http://dx.doi.org/10.1152/ajpheart.01294.2006.

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The goal of this study was to measure diffusive transport of albumin through artery walls experimentally and to analyze the results theoretically, taking into account the binding of albumin to elastic lamellae. Segments of rabbit aorta were placed in solutions of fluorescently labeled albumin for periods of 30, 60, 90, and 120 min, and the distributions of fluorescence intensity through the arterial media were observed. On average, intensity increased almost linearly with time. Bands of high intensity were observed corresponding to elastin layers within the media. The temporal and spatial variations of intensity were compared with predictions of theoretical models, including effects of albumin binding and hindered diffusion resulting from the complex wall structure. Based on these analyses, it was concluded that the spatial distribution of free albumin within the media equilibrated relatively rapidly, and that the observed linear increase in intensity reflected gradual accumulation of albumin bound to medial elastin layers. The results imply that previous theoretical analyses, in which binding was neglected, substantially underestimated albumin diffusivity in the aortic wall. With respect to stent-associated delivery of inhibitors of vascular cell proliferation, the results suggest that albumin might serve as an “affinity vehicle” for drug delivery to the aorta, by attaching the drug to an abundant component of the artery wall.

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Sidhu, K. S., K. E. Mate, F. C. Molinia, A. M. Glazier et J. C. Rodger. « Secretory proteins from the female reproductive tract of the brushtail possum Trichosurus vulpecula : binding to sperm and effects on sperm survival in vitro ». Reproduction, Fertility and Development 11, n^o 6 (1999) : 329. http://dx.doi.org/10.1071/rd00010.

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Previous studies have demonstrated that co-culture of brushtail possum epididymal spermatozoa with oviduct epithelial cell monolayers prolongs sperm survival and results in the re-orientation of the sperm head and tail to the T-shape (thumbtack) configuration. Transformation of sperm to thumbtack orientation is believed to be associated with marsupial sperm capacitation. Here we report that incubation in oviduct-conditioned media also significantly prolongs sperm survival and results in the transformation of sperm to the thumbtack orientation. The major objective of the current study was to examine the proteins present in the conditioned media, to determine whether any of these proteins specifically bound to sperm and the relationship between these proteins and sperm survival and thumbtack orientation. Co-culturing brushtail possum sperm with biotin-labeled proteins in conditioned media (CM) from ampulla, isthmus and uterine explants demonstrated strong binding of two proteins of molecular mass 230 and 61 kD and weak binding of nine proteins of molecular mass 200, 180, 120, 140, 55, 52, 48, 34, 30 kD to sperm within 30 min. The binding of the 61-kD protein from the conditioned media appeared specific as increasing concentrations of non-labeled oviduct proteins, but not serum proteins, inhibited the binding of labeled proteins. The binding of oviduct and uterine proteins in the conditioned media significantly prolonged sperm survival and percentage motility and also transformed a large number of sperm to a thumbtack orientation. The implication of binding of these proteins is discussed in the context of sperm survival and capacitation in this species.

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Syarafina, Zatta Yumni Ihdhar, Mega Safithri, Maria Bintang et Rini Kurniasih. « In Silico Screening of Cinnamon (Cinnamomum burmannii) Bioactive Compounds as Acetylcholinesterase Inhibitors ». Jurnal Kimia Sains dan Aplikasi 25, n^o 3 (8 mars 2022) : 97–107. http://dx.doi.org/10.14710/jksa.25.3.97-107.

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Alzheimer’s is a progressive and neurodegenerative disease that mainly affects people aged 65 years and older. The pathophysiology of Alzheimer’s is possibly related to the depletion of the neurotransmitter acetylcholine (ACh) due to beta-amyloid plaques and neurofibrillary tangles. Secondary metabolites found in cinnamon bark (Cinnamomum burmannii) have the potential as anticholinesterases to treat Alzheimer’s symptoms. This study aimed to identify the potency of bioactive compounds from cinnamon bark as AChE inhibitors in silico through analysis of binding energy, inhibition constants, and types of interactions. The research was conducted by screening virtually 60 test ligands using the PyRx program and molecular docking using the Autodock Tools program. The results of the ligand-receptor interaction analysis showed that 12 of the 15 tested ligands had potential as AChE inhibitors. Epicatechin and medioresinol are the ligands with the best potential for AChE inhibition with affinity close to the natural ligand or donepezil. Epicatechin has a binding energy of −10.0 kcal/mol and inhibition constant of 0.0459 M, with four hydrogen bonds and seven hydrophobic bonds. Meanwhile, medioresinol has −9.9 kcal/mol binding energy and inhibition constant of 0.0543 M, with one hydrogen bond and thirteen hydrophobic bonds.

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Ribes, JA, et CW Francis. « Multimer size dependence of von Willebrand factor binding to crosslinked or noncrosslinked fibrin ». Blood 75, n^o 7 (1 avril 1990) : 1460–65. http://dx.doi.org/10.1182/blood.v75.7.1460.1460.

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Abstract von Willebrand factor (vWF) is synthesized in endothelial cells (EC) and may be either secreted constitutively or stored in Weibel-Palade bodies (WPB) for regulated release. Because fibrin stimulates rapid vWF release from EC, we examined the binding of EC synthesized vWF to fibrin. Culture medium containing constitutively secreted vWF was removed from metabolically labeled primary cultures of human umbilical vein EC, and vWF released from WPB was obtained after stimulation by A23187. vWF-deficient fibrinogen with or without factor XIII was added to releasate or media and clotted with thrombin to form crosslinked or noncrosslinked fibrin. vWF was immunopurified from releasate or media before and after clotting, and the amount and multimeric pattern of vWF bound was determined after sodium dodecyl sulfate agarose gel electrophoresis. High molecular weight multimers of vWF, whether secreted constitutively or released from WPB, bound preferentially to fibrin. Multimers of greater than 20 subunits represented 60% +/- 4% (SEM) of A23187 released vWF and 11% +/- 5% of media vWF, but binding to fibrin was similar, 96% +/- 1% and 94% +/- 2%, respectively. A progressively smaller proportion of vWF bound as multimer size decreased, and dimeric vWF binding was least, with 34% +/- 5% binding from A23187 releasate and 51% +/- 4% from media. The amount of vWF binding to crosslinked or noncrosslinked fibrin was similar, and preferential binding of high molecular weight multimers occurred with both. As measured by enzyme-linked immunosorbent assay, 45% +/- 2% of constitutively secreted vWF bound to crosslinked fibrin and 50% +/- 2% to noncrosslinked fibrin. The propolypeptide of vWF did not bind to fibrin. These findings indicate that binding of EC secreted vWF binding to fibrin depends on multimeric size but not on factor XIII crosslinking. This suggests that vWF released from EC in the presence of fibrin will bind locally, thereby facilitating platelet adhesion to the hemostatic plug or thrombus.

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Ribes, JA, et CW Francis. « Multimer size dependence of von Willebrand factor binding to crosslinked or noncrosslinked fibrin ». Blood 75, n^o 7 (1 avril 1990) : 1460–65. http://dx.doi.org/10.1182/blood.v75.7.1460.bloodjournal7571460.

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von Willebrand factor (vWF) is synthesized in endothelial cells (EC) and may be either secreted constitutively or stored in Weibel-Palade bodies (WPB) for regulated release. Because fibrin stimulates rapid vWF release from EC, we examined the binding of EC synthesized vWF to fibrin. Culture medium containing constitutively secreted vWF was removed from metabolically labeled primary cultures of human umbilical vein EC, and vWF released from WPB was obtained after stimulation by A23187. vWF-deficient fibrinogen with or without factor XIII was added to releasate or media and clotted with thrombin to form crosslinked or noncrosslinked fibrin. vWF was immunopurified from releasate or media before and after clotting, and the amount and multimeric pattern of vWF bound was determined after sodium dodecyl sulfate agarose gel electrophoresis. High molecular weight multimers of vWF, whether secreted constitutively or released from WPB, bound preferentially to fibrin. Multimers of greater than 20 subunits represented 60% +/- 4% (SEM) of A23187 released vWF and 11% +/- 5% of media vWF, but binding to fibrin was similar, 96% +/- 1% and 94% +/- 2%, respectively. A progressively smaller proportion of vWF bound as multimer size decreased, and dimeric vWF binding was least, with 34% +/- 5% binding from A23187 releasate and 51% +/- 4% from media. The amount of vWF binding to crosslinked or noncrosslinked fibrin was similar, and preferential binding of high molecular weight multimers occurred with both. As measured by enzyme-linked immunosorbent assay, 45% +/- 2% of constitutively secreted vWF bound to crosslinked fibrin and 50% +/- 2% to noncrosslinked fibrin. The propolypeptide of vWF did not bind to fibrin. These findings indicate that binding of EC secreted vWF binding to fibrin depends on multimeric size but not on factor XIII crosslinking. This suggests that vWF released from EC in the presence of fibrin will bind locally, thereby facilitating platelet adhesion to the hemostatic plug or thrombus.

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Moreno, Maria João, Luís M. S. Loura, Jorge Martins, Armindo Salvador et Adrian Velazquez-Campoy. « Analysis of the Equilibrium Distribution of Ligands in Heterogeneous Media–Approaches and Pitfalls ». International Journal of Molecular Sciences 23, n^o 17 (28 août 2022) : 9757. http://dx.doi.org/10.3390/ijms23179757.

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The equilibrium distribution of small molecules (ligands) between binding agents in heterogeneous media is an important property that determines their activity. Heterogeneous systems containing proteins and lipid membranes are particularly relevant due to their prevalence in biological systems, and their importance to ligand distribution, which, in turn, is crucial to ligand’s availability and biological activity. In this work, we review several approaches and formalisms for the analysis of the equilibrium distribution of ligands in the presence of proteins, lipid membranes, or both. Special attention is given to common pitfalls in the analysis, with the establishment of the validity limits for the distinct approaches. Due to its widespread use, special attention is given to the characterization of ligand binding through the analysis of Stern–Volmer plots of protein fluorescence quenching. Systems of increasing complexity are considered, from proteins with single to multiple binding sites, from ligands interacting with proteins only to biomembranes containing lipid bilayers and membrane proteins. A new formalism is proposed, in which ligand binding is treated as a partition process, while considering the saturation of protein binding sites. This formalism is particularly useful for the characterization of interaction with membrane proteins.

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Oshovsky, Gennady V., Willem Verboom et David N. Reinhoudt. « (Thio)Urea-Functionalized Cavitands as Excellent Receptors for Organic Anions in Polar Media ». Collection of Czechoslovak Chemical Communications 69, n^o 5 (2004) : 1137–48. http://dx.doi.org/10.1135/cccc20041137.

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Ureidocavitand 1 and thioureidocavitand 2 bind in CH3CN organic anions such as acetate, propionate, butyrate, etc. with K values of 2-8 × 105 l mol-1 and 2-9 × 106 l mol-1, respectively, as was determined with isothermal microcalorimetry (ITC). Bringing together four (thio)urea binding sites on a molecular platform gives rise to about 2000 times higher binding constants, compared with those of the corresponding single binding sites. Glucose- and galactose-containing thioureidocavitands 5 and 6 bind acetate in 1:1 CH3CN/water with a K-value of 2.15 × 103 l mol-1.

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Desnoyers, Luc, David Arnott et Diane Pennica. « WISP-1 Binds to Decorin and Biglycan ». Journal of Biological Chemistry 276, n^o 50 (11 octobre 2001) : 47599–607. http://dx.doi.org/10.1074/jbc.m108339200.

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Wnt-1-induced secreted protein 1 (WISP-1) is a member of the CCN (connective tissue growth factor,Cyr61,NOV) family of growth factors. Structural and experimental evidence suggests that CCN family member activities are modulated by their interaction with sulfated glycoconjugates. To elucidate the mechanism of action for WISP-1, we characterized the specificity of its tissue and cellular interaction and identified binding factors. WISP-1 binding was restricted to the stroma of colon tumors and to cells with a fibroblastic phenotype. By using a solid phase assay, we showed that human skin fibroblast conditioned media contained WISP-1 binding factors. Competitive inhibition with different glycosaminoglycans and treatment with glycosaminoglycan lyases and proteases demonstrated that binding to the conditioned media was mediated by dermatan sulfate proteoglycans. Mass spectrometric analysis identified the isolated binding factors as decorin and biglycan. Decorin and biglycan interacted directly with WISP-1 and inhibited its binding to components in the conditioned media. Similarly, WISP-1 interaction with human skin fibroblasts was inhibited by dermatan sulfate, decorin, and biglycan or by treatment of the cell surface with dermatan sulfate-specific lyases. Together these results demonstrate that decorin and biglycan are WISP-1 binding factors that can mediate and modulate its interaction with the surface of fibroblasts. We propose that this specific interaction plays a role in the regulation of WISP-1 function.

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Kuzmenko, Y. S., V. N. Bochkov, M. P. Philippova, V. A. Tkachuk et T. J. Resink. « Characterization of an atypical lipoprotein-binding protein in human aortic media membranes by ligand blotting ». Biochemical Journal 303, n^o 1 (1 octobre 1994) : 281–87. http://dx.doi.org/10.1042/bj3030281.

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By use of ligand-blotting techniques, this study investigated lipoprotein-binding proteins in human aortic smooth muscle. PAGE was performed under non-reducing conditions, and, using low-density lipoprotein (LDL) as ligand, with rabbit anti-apolipoprotein (apo) B and 125I-labelled goat anti-rabbit IgG as primary and secondary antibodies respectively, we demonstrate that membranes from human aortic media (and cultured human smooth-muscle cells) contain a major lipoprotein-binding protein with an apparent molecular mass of 105 kDa. Anionized preparations (carbamoyl- and acetyl-) of LDL, which did not displace 125I-LDL bound to the apo B,E receptor of cultured fibroblasts, were also recognized as ligands for the 105 kDa protein in aortic media membranes. LDL binding to 105 kDa protein was decreased in the presence of high density lipoprotein (HDL), although more than 100-fold molar excess of HDL was required to achieve 50% displacement of bound LDL. The LDL-binding activity of 105 kDa protein was inhibited by EDTA, and was also significantly decreased when samples were reduced by beta-mercaptoethanol before electrophoresis. Monoclonal antibodies against apo B,E receptor reacted with partially purified bovine adrenal apo B,E receptor, but not with 105 kDa protein of human aortic media membranes. The spectrum of properties of this vascular smooth-muscle lipoprotein-binding protein binding are clearly distinct from those of other previously characterized lipoprotein-binding molecules.

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Larson, Londa J., Kyeong-Sook Kim Shin et Jeffrey I. Zink. « Photoluminescence Spectroscopy of Natural Resins and Organic Binding Media of Paintings ». Journal of the American Institute for Conservation 30, n^o 1 (1991) : 89. http://dx.doi.org/10.2307/3179519.

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Eskew, Matthew W., et Albert S. Benight. « Thermal analysis of protein stability and ligand binding in complex media ». Thermochimica Acta 715 (septembre 2022) : 179274. http://dx.doi.org/10.1016/j.tca.2022.179274.

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Sane, David C., Tammy L. Moser, Thomas M. Bashore, Carrie L. Wagner et Charles S. Greenberg. « Diatrizoate-containing contrast media inhibit platelet aggregation by disrupting fibrinogen binding ». Coronary Artery Disease 2, n^o 3 (mai 1991) : 389–96. http://dx.doi.org/10.1097/00019501-199105000-00013.

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Loizidou, Eriketi, Constantinos Zeinalipour-Yazdi et Lifang Sun. « Artificial Receptor-Attached Amphiphilic Copolymer for Barbiturate Binding in Aqueous Media ». Biomacromolecules 5, n^o 5 (septembre 2004) : 1647–52. http://dx.doi.org/10.1021/bm0497320.

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Larson, Londa J., Kyeong-Sook Kim Shin et Jeffrey I. Zink. « Photoluminescence Spectroscopy of Natural Resins and Organic Binding Media of Paintings ». Journal of the American Institute for Conservation 30, n^o 1 (janvier 1991) : 89–104. http://dx.doi.org/10.1179/019713691806373231.

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Linley, Stuart, Andrew Holmes, Timothy Leshuk, Wanis Nafo, Neil R. Thomson, Adil Al-Mayah, Kevin McVey, Kanwartej Sra et Frank X. Gu. « Targeted nanoparticle binding & ; detection in petroleum hydrocarbon impacted porous media ». Chemosphere 215 (janvier 2019) : 353–61. http://dx.doi.org/10.1016/j.chemosphere.2018.10.046.

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PRELLNER, KARIN. « BACTERIA ASSOCIATED WITH ACUTE OTITIS MEDIA HAVE HIGH Clq BINDING CAPACITY ». Acta Pathologica Microbiologica Scandinavica Section C Immunology 88C, n^o 1-6 (15 août 2009) : 187–90. http://dx.doi.org/10.1111/j.1699-0463.1980.tb00093.x.

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Bonaduce, Ilaria, Leslie Carlyle, Maria Perla Colombini, Celia Duce, Carlo Ferrari, Erika Ribechini, Paola Selleri et Maria Rosaria Tiné. « A multi-analytical approach to studying binding media in oil paintings ». Journal of Thermal Analysis and Calorimetry 107, n^o 3 (1 mai 2011) : 1055–66. http://dx.doi.org/10.1007/s10973-011-1586-6.

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Bonaduce, Ilaria, Catharina Blaensdorf, Patrick Dietemann et Maria Perla Colombini. « The binding media of the polychromy of Qin Shihuang's Terracotta Army ». Journal of Cultural Heritage 9, n^o 1 (janvier 2008) : 103–8. http://dx.doi.org/10.1016/j.culher.2007.08.002.

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Guttmann, Márta Júlia. « Transylvanian glass icons : A GC/MS study on the binding media ». Journal of Cultural Heritage 14, n^o 5 (septembre 2013) : 439–47. http://dx.doi.org/10.1016/j.culher.2012.10.009.

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Zhao, Lan, Kai Zhu, Yongdong Huang, Qiang Li, Xiunan Li, Rongyue Zhang, Zhiguo Su, Qibao Wang et Guanghui Ma. « Enhanced binding by dextran-grafting to Protein A affinity chromatographic media ». Journal of Separation Science 40, n^o 7 (15 mars 2017) : 1493–99. http://dx.doi.org/10.1002/jssc.201601196.

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Liu, Wei, Daniel A. Pasek et Gerhard Meissner. « Modulation of Ca2+-gated cardiac muscle Ca2+-release channel (ryanodine receptor) by mono- and divalent ions ». American Journal of Physiology-Cell Physiology 274, n^o 1 (1 janvier 1998) : C120—C128. http://dx.doi.org/10.1152/ajpcell.1998.274.1.c120.

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The effects of mono- and divalent ions on Ca2+-gated cardiac muscle Ca2+-release channel (ryanodine receptor) activity were examined in [3H]ryanodine-binding measurements. Ca2+ bound with the highest apparent affinity to Ca2+activation sites in choline chloride medium, followed by KCl, CsCl, NaCl, and LiCl media. The apparent Ca2+ binding affinities of Ca2+ inactivation sites were lower in choline chloride and CsCl media than in LiCl, NaCl, and KCl media. Sr2+ activated the ryanodine receptor with a lower efficacy than Ca2+. Competition studies indicated that Li+, K+, Mg2+, and Ba2+ compete with Ca2+ for Ca2+ activation sites. In 0.125 M KCl medium, the Ca2+ dependence of [3H]ryanodine binding was modified by 5 mM Mg2+ and 5 mM β,γ-methyleneadenosine 5′-triphosphate (a nonhydrolyzable ATP analog). The addition of 5 mM glutathione was without appreciable effect. Substitution of Cl−by 2-( N-morpholino)ethanesulfonic acid ion caused an increase in the apparent Ca2+affinity of the Ca2+ inactivation sites, whereas an increase in KCl concentration had the opposite effect. These results suggest that cardiac muscle ryanodine receptor activity may be regulated by 1) competitive binding of mono- and divalent cations to Ca2+ activation sites, 2) binding of monovalent cations to Ca2+ inactivation sites, and 3) binding of anions to anion regulatory sites.

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Fukushi, K., S. Kato, T. Antsuki et T. Omura. « Isolation of copper-binding proteins from activated sludge culture ». Water Science and Technology 44, n^o 2-3 (1 juillet 2001) : 453–59. http://dx.doi.org/10.2166/wst.2001.0801.

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Six copper-binding microbial proteins were isolated from activated sludge cultures grown on media containing copper at various concentrations. Molecular weights among isolated proteins were ranged from 1.3k to 174k dalton. Isolated proteins were compared for their copper binding capabilities. Proteins isolated from cultures grown in the presence of copper in the growth media exhibited higher copper binding capabilities than those isolated from the culture grown in the absence of copper. The highest metal uptake of 61.23 (mol copper/mol protein) was observed by a protein isolated from a culture grown with copper at a concentration of 0.25 mM. This isolated protein (CBP2) had a molecular weight of 24k dalton. Other protein exhibited copper binding capability of 4.8-32.5 (mol copper/mol protein).

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Hayakawa, K., Y. Okuno, K. Fujiwara et Y. Shimizu. « Effect of Iodinated Contrast Media on Ionic Calcium ». Acta Radiologica 35, n^o 1 (janvier 1994) : 83–87. http://dx.doi.org/10.1177/028418519403500117.

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This study was conducted to characterize the alterations in serum ionized calcium induced by contrast media (CM) and to determine the cause of the change. Fresh human blood samples as well as an electrolyte solution (ES) containing 1.2 mM calcium ions were diluted with CM and alterations in ionized calcium were measured using an electrometer. The CM tested were diatrizoate, iopamidol, iohexol, ioxaglate, isotonic saline, Na2-Ca-EDTA and pure meglumine. When CM were mixed with blood, ionic CM produced a greater decrease in ionized calcium than nonionic CM or saline. When mixed with ES, a significant reduction of ionized calcium was induced by ioxaglate and diatrizoate in comparison with saline. Na2-Ca-EDTA and meglumine caused no reduction in ionized calcium. Ioxaglate and diatrizoate caused calcium binding due to anions; binding induced by the latter was more prominent than that by the former. These observations suggest that a nonionic CM is more desirable for coronary arteriography of high-risk patients.

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Farkas, Jeffrey, Melissa S. Liebling et Henry A. Pritzker. « Reactions to Iodinated Contrast Media ». Pediatrics In Review 17, n^o 11 (1 novembre 1996) : 385–86. http://dx.doi.org/10.1542/pir.17.11.385.

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The exact mechanism responsible for adverse reactions to iodinated contrast administered intravenously during radiologic evaluation is not known. However, through work that has been performed both in the laboratory and in clinical trials, we do know that the types of reactions are variable and the underlying etiologies multifactorial. Moreover, reactions can be prevented in many cases through careful choice of contrast agent and pretreatment with corticosteroids and antihistamines. Adverse contrast reactions can be separated into two broad categories: chemotoxic and anaphylactoid. Chemotoxic reactions are dose-dependent and related to direct chemical effects of the contrast medium. Pain at the injection site and flushing belong in this category; a combination of hypertonicity and calcium binding results in vasodilatation.

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Neely, E. Kirk, Stephen D. Smith et Ron G. Rosenfeld. « Human leukemic T and B lymphoblasts produce insulin-like growth factor binding proteins 2 and 4 ». Acta Endocrinologica 124, n^o 6 (juin 1991) : 707–14. http://dx.doi.org/10.1530/acta.0.1240707.

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Abstract. The production of insulin-like growth factors and insulin-like growth factor binding proteins by twelve human leukemic lymphoblast cell lines was evaluated by radioimmunoassay, affinity cross-linking, ligand blot, and immunoprecipitation of conditioned media. In all cell lines, detectable IGF-I and IGF-II levels were <0.1 μg/l and <0.3 μg/l, respectively. IGF binding proteins were identified in 6/12 of the lymphoblast cell lines studied. A pair of IGF binding proteins at 31 and 33 kD, immunoprecipitated with an antibody recognizing IGF binding protein 2 but not by an IGF binding protein 3 antibody, was produced by both T and B cells. A 24 kD IGF binding protein, presumably IGF binding protein 4, since it was not recognized by the antibodies for IGF binding proteins 1, 2, and 3, was produced by B cell precursor cells and faintly by one T cell line. These IGF binding proteins were not altered by deglycosylation. Neither IGF binding protein 1 nor IGF binding protein 3 was identified in any of the conditioned media. We speculate that local production of IGF binding proteins 2 and 4 regulates access of the IGFs to lymphoblasts and to hematopoietic precursors in general.

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Komorowicz, Erzsebet, Robert McBane, Jon Charlesworth et David Fass. « Reduced High Shear Platelet Adhesion to the Vascular Media : Defective von Willebrand Factor Binding to the Interstitial Collagen ». Thrombosis and Haemostasis 87, n^o 04 (2002) : 763–70. http://dx.doi.org/10.1055/s-0037-1613077.

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SummaryFibrillar collagen serves as a thrombogenic surface for platelet adhesion mediated by von Willebrand factor (vWf) at high shear. Although abundant throughout the arterial wall, vWf-dependent platelet deposition to artery cross-sections from perfused citrated blood is localized to the adventitia of the vessel wall. Here we describe a similarly skewed distribution of vWf-binding sites in artery cross-sections. Binding of vWf-coated fluorescent beads, as well as detection of plasma vWf bound to artery cross-section at 3350 s−1 shear rate with indirect particle-immunofluorescence or immunoelectron microscopy demonstrate vWf binding sites in the adventitia, but not in the media. A monoclonal anti-vWf antibody that interferes with vWf-binding to collagen in a microplate ELISA inhibits vWf-binding to both the adventitia and sections of collagen fibrils. Our data suggest that the media, despite its fibrillar collagen content, evidenced by electron microscopy, is defective for vWf-binding, which may explain its thromboresistant nature at high shear rates.

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Sobenin, Igor A., Igor V. Suprun, Vasiliy P. Karagodin, Alexander S. Feoktistov, Alexandra A. Melnichenko et Alexander N. Orekhov. « The Interaction of Plasma Sialylated and Desialylated Lipoproteins with Collagen from the Intima and Media of Uninvolved and Atherosclerotic Human Aorta ». Journal of Lipids 2011 (2011) : 1–8. http://dx.doi.org/10.1155/2011/254267.

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We have evaluated the binding of sialylated and desialylated lipoproteins to collagen isolated from the proteoglycan and musculoelastic layers of intima and media of uninvolved human aorta and atherosclerotic lesions. Comparing various collagen preparations from the uninvolved intima-media, the binding of sialylated apoB-containing lipoproteins was best to collagen from the intimal PG-rich layer. Binding of sialylated apoB-containing lipoproteins to collagen from this layer of fatty streak and fibroatheroma was 1.4- and 3.1-fold lower, respectively, in comparison with normal intima. Desialylated VLDL versus sialylated one exhibited a greater binding (1.4- to 3.0-fold) to all the collagen preparations examined. Desialylated IDL and LDL showed a higher binding than sialylated ones when collagen from the intimal layers of fibroatheroma was used. Binding of desialylated HDL to collagen from the intimal PG-rich layer of normal tissue, initial lesion, and fatty streak was 1.2- to 2.0-fold higher compared with sialylated HDL.

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Datuk Imam Marzuki, Nasrillah et Mhd. Hasbi. « Journalist Philosophy : Binding the Norms of Islamic Law ». Konfrontasi : Jurnal Kultural, Ekonomi dan Perubahan Sosial 9, n^o 2 (31 mai 2022) : 206–15. http://dx.doi.org/10.33258/konfrontasi2.v9i2.208.

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Journalism is related to diaries that are published to the public and published in a certain way. Muslim journalism also breathes prophetic journalism, a form of journalism that not only reports news and problems in a complete, clear, honest, and actual way, but also provides interpretations and directions for change. ,transformation, based on the ethical and prophetic ideals of Islam. He became a journalism that consciously and responsibly contained the values and love of Islam. The philosophy of a journalist who is Muslim, ideally as a continuation of the Prophet's treatise is seen as an important study in today's information age. This is based on the view that the journalist is a teacher from the information society. then becomes important information that is absorbed by the public through the mass media. Because of his strategic position, he can become a benevolent spreader in the community through his mass media.

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Pavlovic, Jelena, Jelena Milenkovic et Nevenka Rajic. « Modification of natural clinoptilolite for the nitrate removal from water media ». Journal of the Serbian Chemical Society 79, n^o 10 (2014) : 1309–22. http://dx.doi.org/10.2298/jsc140116038p.

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The natural zeolitic tuff from the Zlatokop mine (Vranjska Banja deposit) has been investigated as a starting material for obtaining a low cost adsorbent for the removal of nitrate from water media. The tuff riched with zeolite - clinoptilolite was modified by simple procedures by several oxides: iron(III), manganese(IV) and magnesium in order to make clinoptilolite surface accessible for binding the nitrate ions. The obtained oxide-modified zeolite samples were characterized by scanning electron microscopy and energy dispersive X-ray analysis (SEM/EDS), powder X-ray diffraction (PXRD), infrared spectroscopy (FTIR) and by measurement of specific surface area. The effects of the adsorbent dosage (0.5, 1.0, 1.5 and 2.0 g), temperature (25, 35 and 45 ?C) and initial nitrate concentration (C0= 100, 200 and 300 mg dm-3) on the binding efficiency were also studied. For all adsorbents the efficiency increases with temperature. The type of oxides effects the adsorption mechanism and Fe(III) oxide modified zeolite exhibits the best binding ability. For this adsorbent the adsorption kinetics was studied and found that it is best represented by the pseudo-second-order model.

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Tanley, Simon W. M., Antoine M. M. Schreurs, Loes M. J. Kroon-Batenburg et John R. Helliwell. « Room-temperature X-ray diffraction studies of cisplatin and carboplatin binding to His15 of HEWL after prolonged chemical exposure ». Acta Crystallographica Section F Structural Biology and Crystallization Communications 68, n^o 11 (26 octobre 2012) : 1300–1306. http://dx.doi.org/10.1107/s1744309112042005.

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The anticancer complexes cisplatin and carboplatin are known to bind to both the Nδand the N∊atoms of His15 of hen egg-white lysozyme (HEWL) in the presence of dimethyl sulfoxide (DMSO). However, neither binds in aqueous media after 4 d of crystallization and crystal growth, suggesting that DMSO facilitates cisplatin/carboplatin binding to the N atoms of His15 by an unknown mechanism. Crystals of HEWL cocrystallized with cisplatin in both aqueous and DMSO media, of HEWL cocrystallized with carboplatin in DMSO medium and of HEWL cocrystallized with cisplatin andN-acetylglucosamine (NAG) in DMSO medium were stored for between seven and 15 months. X-ray diffraction studies of these crystals were carried out on a Bruker APEX II home-source diffractometer at room temperature. Room-temperature X-ray diffraction data collection removed the need for cryoprotectants to be used, ruling out any effect that the cryoprotectants might have had on binding to the protein. Both cisplatin and carboplatin still bind to both the Nδand N∊atoms of His15 in DMSO media as expected, but more detail for the cyclobutanedicarboxylate (CBDC) moiety of carboplatin was observed at the N∊binding site. However, two molecules of cisplatin were now observed to be bound to His15 in aqueous conditions. The platinum peak positions were identified using anomalous difference electron-density maps as a cross-check withFo−FcOMIT electron-density maps. The occupancies of each binding site were calculated usingSHELXTL. These results show that over time cisplatin binds to both N atoms of His15 of HEWL in aqueous media, whereas this binding is speeded up in the presence of DMSO. The implication of cisplatin binding to proteins after a prolonged period of time is an important consideration for the length of treatment in patients who are given cisplatin.

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Nevin, Austin, et Demetrios Anglos. « Assisted Interpretation of Laser-Induced Fluorescence Spectra of Egg-Based Binding Media Using Total Emission Fluorescence Spectroscopy ». Laser Chemistry 2006 (28 décembre 2006) : 1–5. http://dx.doi.org/10.1155/2006/82823.

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Laser-induced fluorescence (LIF) spectroscopy can provide nondestructive, qualitative analysis of protein-based binding media found in artworks. Fluorescence emissions from proteins in egg yolk and egg white are due to autofluorescent aromatic amino acids as well as other native and age-related fluorophores, but the potential of fluorescence spectroscopy for the differentiation between binding media is dependent on the choice of a suitable excitation wavelength and limited by problems in interpretation. However, a better understanding of emission spectra associated with LIF can be achieved following comparisons with total emission fluorescence spectra where a series of consecutive emission spectra are recorded over a specific range. Results using nanosecond UV laser sources for LIF of egg-based binding media are presented which are rationalised following comparisons with total emission spectra. Specifically, fluorescence is assigned to tryptophan and oxidation products of amino acids; in the case of egg yolk, fatty-acid polymerisation and age-related degradation products account for the formation of fluorophores.

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Jang, Kwang-Suk. « Biolabeling and Binding Evaluation of Amphiphilic Nanocrystallopolymers ». Journal of Nanomaterials 2016 (2016) : 1–7. http://dx.doi.org/10.1155/2016/7416532.

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Surfactant-like inorganic-organic hybrid molecules named as nanocrystallopolymers were designed by conjugation of the hydrophilic synthetic poly(amino acid), poly-α,β-(N-(2-hydroxyethyl)l-aspartamide), with hydrophobic inorganic nanoparticles. In aqueous media, amphiphilic nanocrystallopolymers form self-aggregates with unique morphologies. Here, a simple biolabeling method of nanocrystallopolymers was developed. Biotin was selected as a model biomolecule. The specific binding of biotin-labeled nanocrystallopolymers to the targeted surface was evaluated with a surface plasmon resonance sensor.

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