Bibliographies thématiques / Associated bacteria

Littérature scientifique sur le sujet « Associated bacteria »

Auteur : Grafiati
Publié le 25 mai 2024

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Articles de revues sur le sujet "Associated bacteria"

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Venkatesan, Nandakumar, Govindaraj Perumal et Mukesh Doble. « Bacterial resistance in biofilm-associated bacteria ». Future Microbiology 10, no 11 (novembre 2015) : 1743–50. http://dx.doi.org/10.2217/fmb.15.69.

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Manhart, Lisa E., Christine M. Khosropour, Congzhu Liu, Catherine W. Gillespie, Kevin Depner, Tina Fiedler, Jeanne M. Marrazzo et David N. Fredricks. « Bacterial Vaginosis–Associated Bacteria in Men ». Sexually Transmitted Diseases 40, no 12 (décembre 2013) : 944–49. http://dx.doi.org/10.1097/olq.0000000000000054.

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Gouveia, Joao D., Jie Lian, Georg Steinert, Hauke Smidt, Detmer Sipkema, Rene H. Wijffels et Maria J. Barbosa. « Associated bacteria of Botryococcus braunii (Chlorophyta) ». PeerJ 7 (27 mars 2019) : e6610. http://dx.doi.org/10.7717/peerj.6610.

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Botryococcus braunii (Chlorophyta) is a green microalga known for producing hydrocarbons and exopolysaccharides (EPS). Improving the biomass productivity of B. braunii and hence, the productivity of the hydrocarbons and of the EPS, will make B. braunii more attractive for industries. Microalgae usually cohabit with bacteria which leads to the formation of species-specific communities with environmental and biological advantages. Bacteria have been found and identified with a few B. braunii strains, but little is known about the bacterial community across the different strains. A better knowledge of the bacterial community of B. braunii will help to optimize the biomass productivity, hydrocarbons, and EPS accumulation. To better understand the bacterial community diversity of B. braunii, we screened 12 strains from culture collections. Using 16S rRNA gene analysis by MiSeq we described the bacterial diversity across 12 B. braunii strains and identified possible shared communities. We found three bacterial families common to all strains: Rhizobiaceae, Bradyrhizobiaceae, and Comamonadaceae. Additionally, the results also suggest that each strain has its own specific bacteria that may be the result of long-term isolated culture.
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Fredricks, David N., Tina L. Fiedler et Jeanne M. Marrazzo. « Molecular Identification of Bacteria Associated with Bacterial Vaginosis ». New England Journal of Medicine 353, no 18 (3 novembre 2005) : 1899–911. http://dx.doi.org/10.1056/nejmoa043802.

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Venieraki, A., P. Ch Tsalgatidou, D. G. Georgakopoulos, M. Dimou et P. Katinakis. « Swarming motility in plant-associated bacteria ». Hellenic Plant Protection Journal 9, no 1 (1 janvier 2016) : 16–27. http://dx.doi.org/10.1515/hppj-2016-0002.

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Summary Plant-associated environments harbor a huge number of diverse bacteria that compete and/or cooperate for the occupation of the most nutrient-rich ecological niches. Motility, a common trait among bacteria, has long been assumed to provide a survival advantage to skilful bacteria in invading these environments. Bacterial surface motility, such as swarming, a flagella-driven type of surface movement, although mostly observed and studied on agar substrates, is emerging as a major trait involved in many functions of plant-associated bacteria in regard to their ability to colonize and spread on their host. In this review, we address some novel swarming motility strategies, which enable bacteria to colonize, disperse and compete in plant surfaces.
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Yuan, Jin, Qiuyu Zhang, Shihua Chen, Min Yan et Lei Yue. « LC3-Associated Phagocytosis in Bacterial Infection ». Pathogens 11, no 8 (30 juillet 2022) : 863. http://dx.doi.org/10.3390/pathogens11080863.

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LC3-associated phagocytosis (LAP) is a noncanonical autophagy process reported in recent years and is one of the effective mechanisms of host defense against bacterial infection. During LAP, bacteria are recognized by pattern recognition receptors (PRRs), enter the body, and then recruit LC3 onto a single-membrane phagosome to form a LAPosome. LC3 conjugation can promote the fusion of the LAPosomes with lysosomes, resulting in their maturation into phagolysosomes, which can effectively kill the identified pathogens. However, to survive in host cells, bacteria have also evolved strategies to evade killing by LAP. In this review, we summarized the mechanism of LAP in resistance to bacterial infection and the ways in which bacteria escape LAP. We aim to provide new clues for developing novel therapeutic strategies for bacterial infectious diseases.
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Nwachukwu, Blessing Chidinma, Ayansina Segun Ayangbenro et Olubukola Oluranti Babalola. « Elucidating the Rhizosphere Associated Bacteria for Environmental Sustainability ». Agriculture 11, no 1 (18 janvier 2021) : 75. http://dx.doi.org/10.3390/agriculture11010075.

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The abundance of nutrient accumulation in rhizosphere soils has placed the rhizosphere as an “epicenter” of bacterial concentrations. Nonetheless, over the years, little attention has been given to bacterial inoculants and soil-like substrates. The reason is that many farmers and experiments have focused on chemical fertilizers as an approach to improve plant growth and yield. Therefore, we focused on assessing the application of rhizosphere soil and its associated bacteria for biotechnological applications. This review has been structured into major subunits: rhizosphere soil as a treasure trove for bacterial community concentration, biodegradation of lignocellulose for biofuel production, rhizosphere soil and its bacteria as soil amendments, and the role of rhizosphere soil and its bacteria for bioremediation and biofiltration. Hence, the efficient use of rhizosphere soil and its bacteria in an environmentally friendly way can contribute to healthy and sustainable environments.
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Haynes, S., A. C. Darby, T. J. Daniell, G. Webster, F. J. F. van Veen, H. C. J. Godfray, J. I. Prosser et A. E. Douglas. « Diversity of Bacteria Associated with Natural Aphid Populations ». Applied and Environmental Microbiology 69, no 12 (décembre 2003) : 7216–23. http://dx.doi.org/10.1128/aem.69.12.7216-7223.2003.

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ABSTRACT The bacterial communities of aphids were investigated by terminal restriction fragment length polymorphism and denaturing gradient gel electrophoresis analysis of 16S rRNA gene fragments generated by PCR with general eubacterial primers. By both methods, theγ -proteobacterium Buchnera was detected in laboratory cultures of six parthenogenetic lines of the pea aphid Acyrthosiphon pisum and one line of the black bean aphid Aphis fabae, and one or more of four previously described bacterial taxa were also detected in all aphid lines except one of A. pisum. These latter bacteria, collectively known as secondary symbionts or accessory bacteria, comprised three taxa of γ-proteobacteria (R-type [PASS], T-type [PABS], and U-type [PAUS]) and a rickettsia (S-type [PAR]). Complementary analysis of aphids from natural populations of four aphid species (A. pisum [n= 74], Amphorophora rubi [n= 109], Aphis sarothamni [n= 42], and Microlophium carnosum [n= 101]) from a single geographical location revealed Buchnera and up to three taxa of accessory bacteria, but no other bacterial taxa, in each aphid. The prevalence of accessory bacterial taxa varied significantly among aphid species but not with the sampling month (between June and August 2000). These results indicate that the accessory bacterial taxa are distributed across multiple aphid species, although with variable prevalence, and that laboratory culture does not generally result in a shift in the bacterial community in aphids. Both the transmission patterns of the accessory bacteria between individual aphids and their impact on aphid fitness are suggested to influence the prevalence of accessory bacterial taxa in natural aphid populations.
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S, Nisy, Vishnu N, Megha M et Amitha C. « Endophytes Associated with Macrophyte Ceratophyllum Demersum ». International Journal for Research in Applied Science and Engineering Technology 10, no 3 (31 mars 2022) : 2075–78. http://dx.doi.org/10.22214/ijraset.2022.41055.

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Abstract: Endophytes are microorganisms living inside plants which have various types of symbiotic relations and non-symbiotic relations with host plant. The endophytes reside inside a plant without any signs or symptoms in living tissues of their host. Plants without any endophytes are rare, bacteria and fungi have been seen residing inside plants. Endophytes inside fossilized tissues and stems of plants indicates this relationship started during the evolution or formation of higher plants. The Bacterial and fungal endophytes reach the plant tissues from the rhizosphere, phyllosphere or seed of the plant. Endophytes of Ceratophyllum demersum, were isolated by two methods. Endophytic bacteria and fungi were found to reside in the leaves of macrophyte Ceratophyllum demersum. Four fungal isolates and three bacterial isolates were obtained. Results indicates that fungal endophytes were predominant than bacterial endophytes in Ceratophyllum demersum. Keywords: Ceratophyllum demersum, Macrophytes, Endophytic Bacteria, Endophytic Fungi.
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Anteneh, Yitayal S., Qi Yang, Melissa H. Brown et Christopher M. M. Franco. « Antimicrobial Activities of Marine Sponge-Associated Bacteria ». Microorganisms 9, no 1 (14 janvier 2021) : 171. http://dx.doi.org/10.3390/microorganisms9010171.

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The misuse and overuse of antibiotics have led to the emergence of multidrug-resistant microorganisms, which decreases the chance of treating those infected with existing antibiotics. This resistance calls for the search of new antimicrobials from prolific producers of novel natural products including marine sponges. Many of the novel active compounds reported from sponges have originated from their microbial symbionts. Therefore, this study aims to screen for bioactive metabolites from bacteria isolated from sponges. Twelve sponge samples were collected from South Australian marine environments and grown on seven isolation media under four incubation conditions; a total of 1234 bacterial isolates were obtained. Of these, 169 bacteria were tested in media optimized for production of antimicrobial metabolites and screened against eleven human pathogens. Seventy bacteria were found to be active against at least one test bacterial or fungal pathogen, while 37% of the tested bacteria showed activity against Staphylococcus aureus including methicillin-resistant strains and antifungal activity was produced by 21% the isolates. A potential novel active compound was purified possessing inhibitory activity against S. aureus. Using 16S rRNA, the strain was identified as Streptomyces sp. Our study highlights that the marine sponges of South Australia are a rich source of abundant and diverse bacteria producing metabolites with antimicrobial activities against human pathogenic bacteria and fungi.
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Thèses sur le sujet "Associated bacteria"

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Rooks, Michelle Gabrielle. « Microbiome-Targeted Interventions for Colitis-Associated Bacteria ». Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493456.

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Complex interactions between mammalian hosts and their gut microbes have evolved over many millennia and have established a sophisticated communication system that is essential for symbiosis and mutualism. Perturbations to host-microbiota homeostasis in the context of a genetically susceptible host are central to the development of inflammatory bowel disease (IBD). In-depth understanding of the underlying mechanisms that control homeostasis and dysbiosis are essential for determining how to reliably modulate the host-microbiota continuum to prevent and treat disease. However, deciphering whether alterations in the microbiota are a cause or consequence of IBD remains a considerable challenge, as is defining the role of specific microbes in the pathogenesis of disease. This thesis explores the gut microbiome in mouse models of experimental colitis and evaluates the contribution of specific microbial clades and pathways in potentiating mucosal inflammation with the goal of identifying novel microbiome-targeted interventions for disease management. To improve our understanding of microbial dysbiosis and dysfunction in IBD, we use the TRUC (T-bet-/-RAG2-/- ulcerative colitis) mouse model to profile the gut microbiome in active disease versus treatment-induced remission. 16S ribosomal RNA gene surveys of stool from mice treated with antibiotics, immunodulatory therapies, or a fermented-milk dietary intervention reveal microbial features modified among health and disease states. Discriminatory biomarkers of active disease included increased Enterobacteriaceae and shifts from carbohydrate and energy metabolism to pathways favoring bacterial pathogenesis, specifically cell motility and two-component systems. An unexpected observation is a significant enrichment in genes for microbial benzoate degradation in active colitis. Intermediates of benzoate metabolism – catechols – share the same backbone as host catecholamines, which can signal through two-component systems to promote virulence in pathogenic Enterobacteriaceae. Based on expansions in Enterobacteriaceae and increased gene abundances for benzoate degradation, two-component systems, and bacterial motility proteins, we identify a potential signaling axis linking host adrenergic stress with enhanced bacterial virulence in a preclinical model of colitis. Enterobacteriaceae sense and respond to microbiota-generated signals and host-derived catecholamines through the QseBC two-component quorum sensing system. QseC is a membrane-bound sensor kinase that surveys the external milieu and, upon signal detection, activates its cognate response regulator, QseB, to induce expression of virulence genes. To investigate whether blocking QseC signaling could reduce disease severity, we test the effects of a QseC inhibitor (LED209) in the TRUC, Il-10-/-, and dextran sodium-sulfate models of experimental colitis. LED209 attenuates disease across all three models, with the most striking protective effect in TRUC and dextran sodium sulfate-treated mice. LED209 also prevents the expansion of Enterobacteriaceae in Il-10-/-and dextran sodium-sulfate-exposed mice, but not in TRUC mice, indicating a potential difference in microbiota responses based on genetic context. Moreover, measuring catecholamines in cecal content and stool show that LED209 does not significantly affect the luminal catecholamine pool and thus, may not disrupt host or microbial catecholamine metabolism. Collectively, these data show that QseC inhibition can ameliorate disease in distinct models of experimental colitis and suggest a role for QseC-mediated bacterial virulence in the pathogenesis of IBD. Although a single pathogen has not been identified as a causative agent, several bacteria continue to be implicated in the initiation and progression of IBD, including adherent-invasive Escherichia coli (AIEC). As a proof-of-principle, we genetically inactivate qseC in the Crohn’s disease-associated AIEC strain LF82. We show that absence of qseC leads to downregulated virulence gene expression and defects in flagellar assembly and motility in vitro and reduced colonization efficiency in vivo. Overall, these studies provide evidence that QseC may be an upstream virulence node utilized by colitogenic bacteria to survey their host and potentiate disease and may be a useful target for microbiota-directed therapies in IBD.
Biological Sciences in Public Health
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Savio, Claudia. « Symbiotic and associated bacteria in Tephritid flies ». Doctoral thesis, Università degli studi di Padova, 2011. http://hdl.handle.net/11577/3427445.

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The Tephritidae family, commonly known as “fruit flies”, is a large Dipteran family. It includes many notorious agricultural pests, as the olive fly (Bactrocera oleae), the cherry fruit fly (Rhagoletis cerasi) and the walnut husk fly (R. completa). The importance of bacteria in the life history of fruit flies is well-known. In the beginning of last century Petri (1909) was the first to report the presence of symbiotic bacteria within the olive fruit fly (Bactrocera oleae); recently it was designated as “Candidatus Erwinia dacicola”. In Tephritids flies, the bacteria are housed in the midgut and in a specialized intestinal diverticulum, located in the fly head, called oesophageal bulb. In this thesis, some aspects of the relationship between the above mentioned Tephritid flies and bacteria has been investigated, such as the microflora composition, the symbiont genetic variability and the bacterial transmission. The thesis is composed of five studies. The first study analyzes in details the genetic variability of Ca. E. dacicola in various Italian olive fly populations, studying the 16S rRNA gene. The presence of only two symbiont lineages, not coexisting in the same fly individual, was clearly noticed. Interestingly, the olive fly populations of the two main Italian islands, Sicily and Sardinia, are exclusively represented by one of the two lineages, which could suggest a non-random distribution. On the other hand, the peninsular populations show both bacterial haplotypes, in different proportions. No significant correlation was found between the two symbiont haplotypes and the observed host fly haplotypes, providing evidences for a mixed model of vertical and horizontal transmission of the symbiont during the fly life cycle. The second study extends the previous study to a wider range. The presence of only two symbiont haplotypes was still confirmed for the Mediterranean and African populations. Surprisingly the symbiont haplotypes seem to be more related to the territory than the numerous host haplotypes. The third study deals with the identification of the microflora composition of R. completa and R. cerasi. All the life stages of the cherry fruit fly and the adult stage in R. completa have been taken into account, using both culture dependent and independent methods. Bacteria detected within the oesophageal bulbs of both species are affiliated to Enterobacteriaceae family. The results on the bacterial transmission show a different mechanisms respect to the olive fly and the subfamily Tephritinae symbionts. In the fourth study, the Klebsiella spp. strains isolated from the oesophageal bulb of R. completa and Ceratitis capitata were examined for their ability to incorporate the gene encoding green fluorescent protein (GFP). These bacteria were successfully labelled by conjugation with the gfp gene and the gfp gene was stably maintained in the transgenic strains. Moreover, the colonizing ability of gfp-tagged bacteria in the original host was tested. Here a non-invasive technique to monitor the bacterial fate during the fly life stages was used. Gfp-tagged bacteria were successfully ingested by walnut husk flies where they established a stable population in the fly gut over time and throughout developmental stages. This is the first report in Tephritid flies of native engineered bacteria re-introduced in its original host and the shuttle system used in this study could be a useful tool to expand and strengthen the possibility of biological control of the insect pest. The last study is part of Isabel Martinez-Sañudo PhD thesis, for which I contributed to experimental works. The main goal of this study was to analyse the phylogenetic relationships between flies of the Tephritinae subfamily and their symbiotic bacteria. Some species of this subfamily are in effect known to host specific non-culturable symbiont bacteria (‘‘Candidatus Stammerula spp.”) in their midgut. The cophylogenetic analysis reveals the presence of congruence, even if imperfect, between hosts and symbionts. This non-strict congruence is probably due to events such as losses, duplications and hosts switching, which are likely to arise during the biological cycle of the fly in consideration of the extracellular status of these symbionts.
I Tefritidi, noti anche come “fruit fly”, rappresentano una vasta famiglia di Ditteri comprendenti specie dannose per l’agricoltura quali la mosca dell’olivo (Bactrocera oleae), la mosca del ciliegio (Rhagoletis cerasi), la mosca del noce (R. completa) e la mosca mediterranea della frutta (Ceratitis capitata). L’importanza delle associazioni batteriche nella famiglia dei Tefritidi è nota sin da quando Petri, all’inizio del secolo scorso, riportò la presenza di un battere simbionte, in seguito designato “Candidatus Erwinia dacicola”, in un diverticolo del capo della mosca dell’olivo chiamato bulbo esofageo. I successivi studi hanno evidenziato, sia con metodi tradizionali, sia con un approccio di tipo molecolare, lo stretto legame esistente tra batteri e tefritidi, siano essi simbionti ereditari e coevoluti e non coltivabili o semplici batteri associati. Nel presente lavoro sono stati analizzati in dettaglio alcuni aspetti delle relazioni batteriche in alcune specie di tefritidi, quali la variabilità genetica dei simbionti, la composizione della microflora batterica e la presenza di trasmissione attraverso i diversi stadi di sviluppo dell’ospite. La tesi si articola in cinque capitoli. Il primo lavoro analizza la variabilità genetica nel battere simbionte di B. oleae “Ca. Erwinia dacicola” in diverse popolazioni italiane della mosca dell’olivo, usando il gene ribosomale 16S come marcatore. Lo studio ha evidenziato la presenza di soli due aplotipi del simbionte, evidenziando anche che la loro presenza contemporanea all’interno di uno stesso ospite sembra in base a tutti i reperti non essere non essere probabile. La distribuzione di queste due linee batteriche nelle popolazioni di B. oleae sembra inoltre non essere casuale, poiché le popolazioni delle due maggiori isole italiane (Sardegna e Sicilia) ospitano uno o l’altro dei due aplotipi. Al contrario, le popolazioni della penisola ospitano, in proporzioni significativamente diverse, entrambi gli aplotipi del simbionte. Non è emersa una correlazione tra gli aplotipi di “Ca. E. dacicola” e gli aplotipi mitocondriali del loro ospite. Tale risultato potrebbe essere spiegato ammettendo l’esistenza, oltre alla prevalente trasmissione verticale, di accidentali passaggi orizzontali del simbionte. Nel secondo lavoro l’indagine è stata estesa a un areale più ampio circummediterraneo della mosca dell’olivo. I due aplotipi di “Ca Erwinia dacicola” rinvenuti in Italia sono stati riscontrati con frequenze diverse anche in Africa. Inaspettatamente gli aplotipi del simbionte risultano essere più correlati al territorio di quanto non lo siano i numerosi aplotipi mitocondriali dell’ospite. Nel terzo lavoro è stata indagata la composizione della microflora di R. completa e R. cerasi prelevate in natura, analizzandone i diversi stadi di sviluppo sia con approccio tradizionale coltura-dipendente che con approccio molecolare coltura-indipendente. Dal lavoro è emerso che le entità batteriche predominanti presenti nel bulbo esofageo appartengono alla famiglia delle Enterobacteriaceae. I risultati ottenuti evidenziano un meccanismo di trasmissione dei batteri diverso da quello evidenziato per i simbionti della mosca dell’olivo e della sottofamiglia Tephritinae. Nel quarto lavoro è stata studiata l’abilità di ceppi di Klebsiella isolati originariamente dal bulbo esofageo di R. completa e C. capitata di incorporare il gene per l’espressione di una proteina fluorescente (GFP) e quindi la capacità del battere cosi modificato di ri-colonizzare l’ospite originario. Questa tecnica non distruttiva ha consentito il monitoraggio del destino dei batteri nel corso degli stadi di sviluppo dell’insetto. I batteri modificati sono stai ingeriti con successo dalle mosche del noce e ne hanno colonizzato in modo stabile l’intestino medio allo stadio di larva e quindi nella pupa. Si tratta del primo caso in cui un battere tipico della microflora di un tefritide è stato ingegnerizzato con successo e quindi introdotto nell’ospite nativo. La tecnica utilizzata in questo studio potrebbe costituire un valido strumento per espandere questo tipo di ricerca anche al controllo biologico di altre specie dannose in agricoltura. L’ultimo lavoro fa parte parzialmente della tesi di dottorato della Dott.ssa Isabel Martinez-Sañudo, per il quale ho contribuito nella parte sperimentale. L’obbiettivo principale di questo studio è stato quello di indagare le relazioni filogenetiche tra le mosche della sottofamiglia delle Tefritine e i loro batteri simbionti. Alcune specie di questa sottofamiglia sono infatti note per ospitare un simbionte specifico ereditario e non coltivabile (‘‘Candidatus Stammerula spp.”) nell’intestino medio. Tali batteri simbionti sono presenti solo in due delle cinque tribù della sottofamiglia studiate. L’analisi della cofilogenesi ha rivelato la presenza di una congruenza, seppure imperfetta, tra ospiti e simbionti.
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Alvarez, Julia D. « Studies on Venezuelan fish and shrimp associated bacteria ». Thesis, Heriot-Watt University, 1998. http://hdl.handle.net/10399/619.

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Slabbert, Róan Stephanus. « Evaluation of acid resistance in food-associated bacteria ». Thesis, Bloemfontein : Central University of Technology, Free State, 2011. http://hdl.handle.net/11462/147.

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Thesis (M. Tech. Environmental health) -- Central University of technology, Free State, 2011
Although the application of low pH is common practice in food preservation, the emergence of acid tolerance has been reported world-wide amidst a growing concern that preservation with weak acids, such as organic acids may be influenced as a result of food-borne bacteria becoming acid tolerant or acid resistant. The present study was conducted to assess the acid tolerance of a wide range of bacterial species and consequently the sustainable application of organic acids as food preservatives in particularly acidic foodstuffs. Acid tolerance was determined in 19 bacterial strains predominantly associated with food spoilage and food poisoning. After exposure to hydrochloric acid 16% of the isolates were found to be intrinsically tolerant to low pH and included amongst others the enteric bacteria Escherichia coli and Salmonella spp. The latter organisms are known causative agents in food spoilage and poisoning, and the results highlight the predicaments related to their ability to survive in acidic foodstuffs as well as the human gastric environment. Bacterial strains were further exposed to increasing concentrations of various acidic foodstuffs in order to determine the development of acid tolerance by gradual decrease in pH, as opposed to exposure to acid shock. After induction, the protein profiles of resulting acid tolerant isolates were compared with those of the original un-induced strains. Exposure to acidic foodstuffs resulted in various survival profiles, where not only pH but also the type of acidulant (foodstuff or inorganic acid) were found to be contributing factors in acid tolerance development. Bacterial protein composition after exposure to acidic foodstuffs showed considerable variation which may be indicative of acid tolerance development whereas the mechanisms involved may be the result of multiple modifications in bacterial composition. After the induction of acid tolerance, susceptibility of induced strains to various organic acids were determined at various pH values. This was done to investigate whether acid tolerance would influence the inhibitory activity of organic acids as antimicrobial agents in acidic food. Decreased susceptibility was not significantly demonstrated with the exception of only selected isolates, the latter including E. coli and S. typhimurium. Organic acid activity was found to be much more effective at lower pH values and it would be necessary to elucidate whether this inhibition is the result of a lower pH or more specifically the activity of the organic acids. The effect of exposure to an acidic environment on phenotypic characteristics of Gram-negative bacteria, and more specifically psychrotrophic organisms was evaluated in order to show the combined effect of organic acids and low temperature preservation. The characteristic yellow pigment of various Chryseobacterium species was found to be not as apparent after acid exposure while in some cases the colonies were observed as white. In Pseudomonas aeruginosa the characteristic green pigment was much more prominent after acid exposure. These morphological alterations may be important factors that should be considered in identification procedures employed in food safety laboratories. Finally, the influence of acidic exposure via acidic foodstuffs and also organic acids on the protein composition and outer membrane protein structure of various bacterial cells was investigated. No specific relationships with the MICs (Minimum Inhibitory Concentrations) of organic acids after induction with the selected acidic foodstuffs could be established, although various differences were found in protein expression. From the results, it may be suggested that the outer membrane of various pathogenic bacteria is involved in acid tolerance development and this supports the reports on the importance of membrane integrity in the protection against low pH. In conclusion, the study endeavoured to add to the body of knowledge with regard to alternative food preservation regimes utilising organic acids, either solely or in combination with selected extrinsic and intrinsic parameters.
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Tyler, Heather Lee. « Plant-associated bacteria biological, genomic, and metagenomic studies / ». [Gainesville, Fla.] : University of Florida, 2009. http://purl.fcla.edu/fcla/etd/UFE0041068.

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Bharadwaj, Dharam Parkash. « The plant - arbuscular mycorrhizal fungi - bacteria - pathogen system : multifunctional role of AMF spore-associated bacteria / ». Uppsala : Dept, of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/200790.pdf.

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Cuschieri, Katie Sarah. « Species diversity of aggregate-associated marine ammonia-oxidising bacteria ». Thesis, University of Aberdeen, 2000. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU602054.

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Two broad communities can be distinguished in marine systems, those attached to amorphous aggregate material dispersed throughout the water column and those that are freely suspended in the water column (planktonic). It has been suggested that two distinct microbial populations are associated with each habitat due to phenotypic adaptation to the different conditions in aggregates and the surrounding water. The aim of this study was to investigate the diversity of aggregate-associated and planktonic marine ammonia oxidisers (AOBs - the organisms responsible for the rate limiting step in nitrification) in both natural environments and laboratory-reared systems and to determine whether aggregate material selected for particular groups of AOBs. Detection of AOBs relied heavily on the use of molecular analysis of extracted DNA. Thus, a preliminary study was performed to assess whether preferential lysis occurred when representatives of both genera within the B-subgroup AOBs {Nitrosospira multiformis and Nitrosomonas europaea) were exposed to lysis procedures commonly applied to marine samples. Minimal bias existed, with Nitrosomonas europaea proving to be less susceptible to lysis only when the lytic agents (sodium dodecyl sulphate and lysozyme) were absent or at concentrations 100-fold less than those applied in routine environmental extraction. Environmental populations of aggregate-associated and planktonic AOBs in the NW Mediterranean Sea were assessed in summer and winter at stations both within and beyond regions of fresh water inflow (the plume). Molecular analysis involved amplification, by the polymerase chain reaction, of 16S rRNA genes from extracted DNA using AOB-specific primers. Analysis of 16S rDNA sequences coupled with DGGE and specific probing revealed temporal and spatial effects in community structure of AOBs. In the summer, genus level selection of AOBs was observed with Nitrosospira dominating in the aggregate population and Nitrosomonas dominating in the planktonic phase. This was found in the surface waters of geographically distant sites within and outside the plume. Between-site differences were evident in the deeper waters with Nitrosospira-like sequences more abundant in plume diluted waters and Nitrosomonas like sequences more abundant outside this zone, while genus level selection between aggregate-associated and planktonic communities was not detected. In winter, a uniform pattern of AOB distribution emerged with an even distribution of two Nitrosospira sequences at each site on all aggregate and planktonic samples. The AOB community structure of sediment samples was not wholly resolved by application of direct molecular techniques and the culturable diversity was later examined by an enrichment-based approach. A laboratory-reared aggregate system was developed to assess the distribution and selection of inoculated pure and enrichment cultures of AOBs and to assess the effect of sampling technique on the observed community structure. Enclosed vessels containing North Sea water were rotated until aggregation of autochthonous particulate material formed discrete aggregates. No genus level selection of AOBs was observed in aggregate-associated and planktonic communities in North Sea water yet differences in the distribution of closely related sequences within cluster 1 Nitrosospira were observed between the two communities. Observed aggregate and planktonic community structure was affected by the method used to separate the two fractions. Active bacterial production was not necessary for aggregate formation if a pooled suspension of aggregates was sterilised and added to a medium of cell-free filtered sea water. Thus, the successful inoculation and retrieval of an N. multiformis culture within the cell free system suggested that it was appropriate for investigation of the colonisation dynamics of inoculated AOBs.
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Ridsdale, Carmen Jane. « Interactions of arbuscular mycorrhizal fungi and spore-associated bacteria ». Thesis, Rhodes University, 2013. http://hdl.handle.net/10962/d1018269.

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Arbuscular mycorrhizal (AM) fungi are naturally occurring in roots of terrestrial plants. AM fungi are capable of benefiting the host plant through various mechanisms such as enhanced nutrient supply, alleviation of environmental stress and inhibition of plant fungal pathogens. AM fungal spore-associated bacteria have been previously isolated and shown to have plant growthpromoting (PGP) abilities by several authors. Some bacterial isolates are able to promote AM fungal colonisation of host plants and are known to be mycorrhizal helper bacteria (MHB). This study focused on the isolation of AM fungal spore-associated bacteria, characterization of the isolates according to plant growth promoting abilities and evaluation of their potential to enhance plant growth and mycorrhizal colonisation. AM fungi were extracted from soils sampled from natural indigenous forest sources, raspberry (Rubus idaeus cv. Heritage) and strawberry (Fragaria ananassa) farms in South Africa and from a raspberry (Rubus idaeus cv. Autumn Bliss) plantation in Argentina. A total of 52 sporeassociated bacteria were isolated from the external and internal surfaces of AM fungal spore morphotypes from the two countries. The bacterial isolates were evaluated for their PGP abilities such as phosphate solubilisation, indole-3-acetic acid production, ammonia production and inhibition of the fungal pathogens Fusarium oxysporum and Phythophthora nicotianae through mechanisms such as siderophore and/ or hydrolytic enzyme production. A total of 23 bacterial isolates from both South Africa and Argentina showing the most potential to be PGP, were identified molecularly as belonging to the genera Acinetobacter, Alcaligenes, Bacillus, Microbacterium, Micrococcus, Serratia and Staphylococcus. The ability of ten selected bacterial isolates showing multiple PGP capacity were evaluated for their plant growth promotion and mycorrhizal colonisation enhancement ability on raspberry (Rubus idaeus cv. Meeker). Significant differences in increased shoot and root dry weights were shown by the treatments compared to the uninoculated control. The highest increase in shoot and root dry weights were shown by South African (Bacillus mycoides) and Argentinean (Alcaligenes faecalis) isolates. AM fungal colonisation was significantly enhanced by the South African (Bacillus mycoides) and Argentinean (Micrococcus luteus) isolates compared to the AM fungal singly inoculated control.
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Fandino, Laura B. « Molecular ecology of bacteria associated with marine phytoplankton blooms / ». Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2002. http://wwwlib.umi.com/cr/ucsd/fullcit?p3064445.

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Ye, Jingxiao. « Intestinal bacteria associated with colitis and inflammatory bowel disease ». Diss., [Riverside, Calif.] : University of California, Riverside, 2009. http://proquest.umi.com/pqdweb?index=0&did=1957340931&SrchMode=2&sid=2&Fmt=2&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1269024239&clientId=48051.

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Thesis (Ph. D.)--University of California, Riverside, 2009.
Includes abstract. Available via ProQuest Digital Dissertations. Title from first page of PDF file (viewed March 20, 2010). Includes bibliographical references. Also issued in print.
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Livres sur le sujet "Associated bacteria"

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S, Gnanamanickam S., dir. Plant-associated bacteria. Dordrecht : Springer, 2006.

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Gnanamanickam, Samuel S., dir. Plant-Associated Bacteria. Dordrecht : Springer Netherlands, 2006. http://dx.doi.org/10.1007/978-1-4020-4538-7.

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Gnanamanickam, Samuel S., dir. Plant-Associated Bacteria. Dordrecht : Kluwer Academic Publishers, 2006. http://dx.doi.org/10.1007/1-4020-4538-7.

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S, Gnanamanickam S., dir. Plant-associated bacteria. Dordrecht : Springer, 2006.

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Gross, Dennis C., Ann Lichens-Park et Chittaranjan Kole, dir. Genomics of Plant-Associated Bacteria. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55378-3.

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Manipulative tenants : Bacteria associated with arthropods. Boca Raton : Taylor & Francis, 2012.

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P, Spaink Herman, Kondorosi A et Hooykaas Paul J. J, dir. The rhizobiaceae : Molecular biology of model plant-associated bacteria. Dordrecht : Kluwer Academic, 1998.

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Luan, Xiujie. Study of the bacteria associated with exacerbation of late-onset asthma. [Derby : University of Derby], 2000.

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1963-, Fernando A., et Pacific Forestry Centre, dir. Index of plant pathogens, plant-associated microorganisms, and forest fungi of British Columbia. Victoria : Pacific Forestry Centre, 1999.

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Peter, Whalley. The proteolytic and saccharolytic activity of some natural waters and their associated bacteria. [s.l.] : typescript, 1987.

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Chapitres de livres sur le sujet "Associated bacteria"

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Höfte, Monica, et Paul De Vos. « Plant pathogenic Pseudomonas species ». Dans Plant-Associated Bacteria, 507–33. Dordrecht : Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-4538-7_14.

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van Loon, L. C., et P. A. H. M. Bakker. « Root-associated bacteria inducing systemic resistance ». Dans Plant-Associated Bacteria, 269–316. Dordrecht : Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-4538-7_8.

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de Weert, Sandra, et Guido V. Bloemberg. « Rhizosphere competence and the role of root colonization in biocontrol ». Dans Plant-Associated Bacteria, 317–33. Dordrecht : Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-4538-7_9.

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Zhao, Youfu. « Genomics of Erwinia amylovora and Related Erwinia Species Associated with Pome Fruit Trees ». Dans Genomics of Plant-Associated Bacteria, 1–36. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55378-3_1.

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Zhao, Yan, Robert E. Davis, Wei Wei, Jonathan Shao et Rasa Jomantiene. « Phytoplasma Genomes : Evolution Through Mutually Complementary Mechanisms, Gene Loss and Horizontal Acquisition ». Dans Genomics of Plant-Associated Bacteria, 235–71. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55378-3_10.

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Charkowski, Amy O., Jenna Lind et Isael Rubio-Salazar. « Genomics of Plant-Associated Bacteria : The Soft Rot Enterobacteriaceae ». Dans Genomics of Plant-Associated Bacteria, 37–58. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55378-3_2.

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Baltrus, David A., Tory A. Hendry et Kevin L. Hockett. « Ecological Genomics of Pseudomonas syringae ». Dans Genomics of Plant-Associated Bacteria, 59–77. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55378-3_3.

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Vinatzer, Boris A., et Caroline L. Monteil. « Pseudomonas syringae Genomics : From Comparative Genomics of Individual Crop Pathogen Strains Toward Population Genomics ». Dans Genomics of Plant-Associated Bacteria, 79–98. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55378-3_4.

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Seaton, Sarah Craven, et Mark W. Silby. « Genetics and Functional Genomics of the Pseudomonas fluorescens Group ». Dans Genomics of Plant-Associated Bacteria, 99–125. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55378-3_5.

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Triplett, Lindsay, Ralf Koebnik, Valerie Verdier et Jan E. Leach. « The Genomics of Xanthomonas oryzae ». Dans Genomics of Plant-Associated Bacteria, 127–50. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55378-3_6.

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Actes de conférences sur le sujet "Associated bacteria"

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Lithgow, K., V. Buchholz, S. Konschuh et L. Sycuro. « O02.5 Secreted Proteolytic Activity of Bacterial Vaginosis-Associated Bacteria ». Dans Abstracts for the STI & HIV World Congress, July 14–17 2021. BMJ Publishing Group Ltd, 2021. http://dx.doi.org/10.1136/sextrans-2021-sti.60.

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Nerlich, Paula. « Sensory textile-bacterial hybrids : textile-bacteria fusion to impart forest-associated scents ». Dans Textile Intersections 2023. Design Research Society, 2023. http://dx.doi.org/10.21606/ti-2023/111.

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Ali, Muna Jalal, et Rasha H. Al-Rikabi. « Antibiotic and virulence profile of UTIs associated bacteria ». Dans INTERNATIONAL CONFERENCE OF NUMERICAL ANALYSIS AND APPLIED MATHEMATICS ICNAAM 2019. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0029707.

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Wibowo, Joko Tri, Senlie Octaviana et Tutik Murniasih. « The potentials of cultivable sea cucumber associated bacteria ». Dans PROCEEDINGS OF THE 9TH INTERNATIONAL SYMPOSIUM ON INNOVATIVE BIOPRODUCTION INDONESIA ON BIOTECHNOLOGY AND BIOENGINEERING 2022 : Strengthening Bioeconomy through Applied Biotechnology, Bioengineering, and Biodiversity. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0183399.

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Iungin, Olga, Ievgeniia Prekrasna, Ihor Bortyanuy, Valeriia Maslak et Saulius Mickevičius. « Plant Growth-Promoting Characteristics of Antarctic Endophytic Bacteria ». Dans The 9th International Conference on Advanced Materials and Systems. INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania, 2022. http://dx.doi.org/10.24264/icams-2022.ii.11.

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The work is focused on studying bacteria associated with vascular plants in Antarctic region. Climate changes affecting the Antarctic Peninsula favor the successful colonization of ice-free lands by two Antarctic vascular plants (Deschampsia antarctica and Colobanthus quitensis). Bacteria isolated from D. antarctica collected during the 25th Ukrainian Antarctic Expedition (January-April 2020) along the Western part of the Antarctic Peninsula were studied for plant growth-promoting characteristics (nitrogen fixation, phosphate solubilization, cyclic lipopeptide production, exoprotease production, motility and carbohydrate utilization). The heterotrophy of bacterial isolated from D. antarctica and the presence of a wide range of saccharolytic enzymes for the utilization of mono- and disaccharides in studied cultures were shown. This may indicate the plasticity of metabolism and the high adaptation potential of microorganisms associated with D. antarctica. PGPT of studied bacteria were mostly presented by nitrogen-fixing ability and cyclic lipopeptides synthesis.
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Golding, P. S., L. Maddocks, T. A. King et D. B. Drucker. « Selective Laser Inactivation of Periodontal Bacteria ». Dans The European Conference on Lasers and Electro-Optics. Washington, D.C. : Optica Publishing Group, 1996. http://dx.doi.org/10.1364/cleo_europe.1996.cfc6.

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Periodontal disease, caused by bacterial biofilms (plaques) on tooth surfaces, is one of the major causes of tooth loss in adults. The current method of treatment involves a labour-intensive, unpleasant scraping away of the plaque from within the periodontal pocket1. With the recognition that this disease is associated with specific organisms, increasing interest is being shown in the use of anti-microbial agents to kill the bacteria in situ.
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R.C. Jamieson, D.M. Joy, H. Lee et S. Willie. « RESUSPENSION OF SEDIMENT-ASSOCIATED BACTERIA IN AN ALLUVIAL STREAM ». Dans 2004, Ottawa, Canada August 1 - 4, 2004. St. Joseph, MI : American Society of Agricultural and Biological Engineers, 2004. http://dx.doi.org/10.13031/2013.17886.

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Srinivasan, S. « S01.1 Identification of bacteria associated with idiopathic nongonococcal urethritis ». Dans Abstracts for the STI & HIV World Congress, July 14–17 2021. BMJ Publishing Group Ltd, 2021. http://dx.doi.org/10.1136/sextrans-2021-sti.19.

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Kumalasari, Yeni Indra, Agung Dian Kharisma et Sri Yuwantiningsih. « Potential of Karimunjawa Island’s Plants as Antibiotic-Producing Endophytic Bacteria Sources ». Dans The 2nd International Conference on Technology for Sustainable Development. Switzerland : Trans Tech Publications Ltd, 2022. http://dx.doi.org/10.4028/p-kv25ou.

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Endophytic bacteria have a great potential to be applied as biofertilizers and biopesticides, but their information as a source of antibiotics still needs to be developed and explored. The aim of this study was to investigate the potential sources of antibiotics in endophytic bacteria isolated from the stems of Setigi, Wahong, Bongko, Kalimosodo, Dewandaru, and Legundi plants on Karimunjawa Island. Molecular approaches were performed to isolate, characterize, and identify bacterial endophytes as potential antibiotic sources by plate assay and 16S rRNA gene sequence analysis. Dewandaru isolate was identified as gram-negative bacteria, whereas; gram-positive bacteria were detected in other isolates. Moreover, Setigi and Dewandaru isolates showed the highest level to inhibit the growth of Fusarium sp and displayed 99% similarity with antibiotic-producing bacteria, namely Bacillus pumilus and Bacillus cereus, respectively. These results indicate the possibility of antibiotic activities by Setigi and Dewandaru isolated. Therefore, it is assumed that both Setigi and Dewandaru isolates potentially appeared as new antibiotics sources from local plants. This study provides novel insight into the future production of novel antibiotics derived from plant-associated endophytic bacterial as a strategy for increasing the application of natural compounds to control plant diseases in agriculture.
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Rodrigues, Francisco, Raquel Teixeira et Patricia Coelho. « Antibiotic consumption in Portugal - An overview ». Dans III SEVEN INTERNATIONAL MULTIDISCIPLINARY CONGRESS. Seven Congress, 2023. http://dx.doi.org/10.56238/seveniiimulti2023-225.

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Infections caused by opportunistic bacteria resistant to multiple antibiotics continue to be a major challenge in the hospital environment. Growing bacterial resistance results in compromising the success of immunosuppressive therapies and surgical interventions (transplantation), which associated with a high risk of bacterial infections during the procedure and post-operatively often culminate in the death of the patient.
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Rapports d'organisations sur le sujet "Associated bacteria"

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Cruz-Perez, P., L. D. Stetzenbach et A. J. Alvarez. PCR detection of groundwater bacteria associated with colloidal transport. Office of Scientific and Technical Information (OSTI), février 1996. http://dx.doi.org/10.2172/237359.

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Sadowski, R. A., G. Chen, C. R. Clayton, J. R. Kearns, J. B. Gillow et A. J. Francis. A Scanning Auger Microprobe analysis of corrosion products associated with sulfate reducing bacteria. Office of Scientific and Technical Information (OSTI), mars 1995. http://dx.doi.org/10.2172/86945.

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Mendoza, Jonathan Alberto, Carolina Mazo, Lina Margarita Conn, Álvaro Rincón Castillo, Daniel Rojas Tapias et Ruth Bonilla Buitrago. Evaluation of phosphate-solubilizing bacteria associated to pastures of Bracharia from acid soils. Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA, 2015. http://dx.doi.org/10.21930/agrosavia.informe.2015.5.

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Rhizobia have been widely known by their capacity to form a symbiotic relationship with legumes and fix atmospheric nitrogen. Recently, however, rhizobia have shown to associate with plants in different botanical families. In this study, we aimed at elucidating the diversity of rhizobia associated to grasses, and determine their capabilities to solubilize phosphate in both lab and greenhouse experiments. Isolation of rhizobia was performed using rhizosphere from Brachiaria brizantha and B. decumbens and a promiscuous legume trap plant (i.e. Vigna unguiculata). Thirty days after inoculation of the trap plant, rhizobia were isolated from nodules using the conventional protocol, classified in basis on their phenotypic features, and molecularly grouped using Amplified Ribosomal DNA Restriction Analysis (ARDRA). Finally, phosphate solubilization assays and greenhouse experiments were carried out on representatives of each ARDRA cluster. The results showed that the diversity of rhizobia varied between both plant species, which suggests that plant exudates significantly determine the composition of the plant microbiome. Surprisingly, most of the isolated associated to B. brizantha rhizosphere exhibited typical attributes of slow-growing rhizobia, whereas rhizobia from B. decumbens displayed a mixed diversity including slow-, intermediate-, and fast-growing rhizobia. Sequencing of 16S rRNA of ARDRA representatives showed that most of the rhizobia isolated from B. brizantha belonged to the Mesorhizobium and Bradyrhizobium genera, while those isolated from B. decumbens were phylogenetically clustered into Rhizobium and Bradyrhizobium. The capability of the isolates to solubilize phosphate was studied using iron and calcium phosphate. We observed that overall Bradyrhizobium exhibited the highest ability to solubilize iron phosphate; by contrast, calcium phosphate was similarly solubilized within representatives of the three genera. In greenhouse experiments, we found that plants inoculated with isolated BT53, BD17 and BD21 exhibited a significantly higher content of phosphorus (p≤0.05). Additionally, dry weight was significantly higher in the treatment inoculated with BT16 isolate (p≤0.05). We conclude that 1) rhizobia is found associated with grasses, 2) plant genotype determines rhizobia diversity 3) rhizobia are able to solubilize phosphorus, and 4) they might be used to promote plant in different plant families. We further believe that further studies will reveal the true role of those old-known legume symbionts in development and growth of other important crops.
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Cytryn, Eddie, Mark R. Liles et Omer Frenkel. Mining multidrug-resistant desert soil bacteria for biocontrol activity and biologically-active compounds. United States Department of Agriculture, janvier 2014. http://dx.doi.org/10.32747/2014.7598174.bard.

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Control of agro-associated pathogens is becoming increasingly difficult due to increased resistance and mounting restrictions on chemical pesticides and antibiotics. Likewise, in veterinary and human environments, there is increasing resistance of pathogens to currently available antibiotics requiring discovery of novel antibiotic compounds. These drawbacks necessitate discovery and application of microorganisms that can be used as biocontrol agents (BCAs) and the isolation of novel biologically-active compounds. This highly-synergistic one year project implemented an innovative pipeline aimed at detecting BCAs and associated biologically-active compounds, which included: (A) isolation of multidrug-resistant desert soil bacteria and root-associated bacteria from medicinal plants; (B) invitro screening of bacterial isolates against known plant, animal and human pathogens; (C) nextgeneration sequencing of isolates that displayed antagonistic activity against at least one of the model pathogens and (D) in-planta screening of promising BCAs in a model bean-Sclerotiumrolfsii system. The BCA genome data were examined for presence of: i) secondary metabolite encoding genes potentially linked to the anti-pathogenic activity of the isolates; and ii) rhizosphere competence-associated genes, associated with the capacity of microorganisms to successfully inhabit plant roots, and a prerequisite for the success of a soil amended BCA. Altogether, 56 phylogenetically-diverse isolates with bioactivity against bacterial, oomycete and fungal plant pathogens were identified. These strains were sent to Auburn University where bioassays against a panel of animal and human pathogens (including multi-drug resistant pathogenic strains such as A. baumannii 3806) were conducted. Nineteen isolates that showed substantial antagonistic activity against at least one of the screened pathogens were sequenced, assembled and subjected to bioinformatics analyses aimed at identifying secondary metabolite-encoding and rhizosphere competence-associated genes. The genome size of the bacteria ranged from 3.77 to 9.85 Mbp. All of the genomes were characterized by a plethora of secondary metabolite encoding genes including non-ribosomal peptide synthase, polyketidesynthases, lantipeptides, bacteriocins, terpenes and siderophores. While some of these genes were highly similar to documented genes, many were unique and therefore may encode for novel antagonistic compounds. Comparative genomic analysis of root-associated isolates with similar strains not isolated from root environments revealed genes encoding for several rhizospherecompetence- associated traits including urea utilization, chitin degradation, plant cell polymerdegradation, biofilm formation, mechanisms for iron, phosphorus and sulfur acquisition and antibiotic resistance. Our labs are currently writing a continuation of this feasibility study that proposes a unique pipeline for the detection of BCAs and biopesticides that can be used against phytopathogens. It will combine i) metabolomic screening of strains from our collection that contain unique secondary metabolite-encoding genes, in order to isolate novel antimicrobial compounds; ii) model plant-based experiments to assess the antagonistic capacities of selected BCAs toward selected phytopathogens; and iii) an innovative next-generation-sequencing based method to monitor the relative abundance and distribution of selected BCAs in field experiments in order to assess their persistence in natural agro-environments. We believe that this integrated approach will enable development of novel strains and compounds that can be used in large-scale operations.
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Chorazyczewski, Adam M., Paul V. Zimba, Xavier Mayali et Joe Fox. Do Phycosphere associated bacteria affect the growth and lipid production of Phaeodactylum tricornutum Bohlin ? Office of Scientific and Technical Information (OSTI), février 2019. http://dx.doi.org/10.2172/1544511.

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Kadouri, Daniel E. The Potential Application and Risks Associated With the Use of Predatory Bacteria as a Biocontrol Agent Against Wound Infections. Fort Belvoir, VA : Defense Technical Information Center, octobre 2013. http://dx.doi.org/10.21236/ada595292.

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Zchori-Fein, Einat, Judith K. Brown et Nurit Katzir. Biocomplexity and Selective modulation of whitefly symbiotic composition. United States Department of Agriculture, juin 2006. http://dx.doi.org/10.32747/2006.7591733.bard.

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Whiteflies are sap-sucking insects that harbor obligatory symbiotic bacteria to fulfill their dietary needs, as well as a facultative microbial community with diverse bacterial species. The sweetpotato whitefly Bemisia tabaci (Gennadius) is a severe agricultural pest in many parts of the world. This speciesconsists of several biotypes that have been distinguished largely on the basis of biochemical or molecular diagnostics, but whose biological significance is still unclear. The original objectives of the project were (i) to identify the specific complement of prokaryotic endosymbionts associated with select, well-studied, biologically and phylogeographically representative biotypes of B. tabaci, and (ii) to attempt to 'cure’ select biotypes of certain symbionts to permit assessment of the affect of curing on whitefly fitness, gene flow, host plant preference, and virus transmission competency.To identify the diversity of bacterial community associated with a suite of phylogeographically-diverseB. tabaci, a total of 107 populations were screened using general Bacteria primers for the 16S rRNA encoding gene in a PCR. Sequence comparisons with the available databases revealed the presence of bacteria classified in the: Proteobacteria (66%), Firmicutes (25.70%), Actinobacteria (3.7%), Chlamydiae (2.75%) and Bacteroidetes (<1%). Among previously identified bacteria, such as the primary symbiont Portiera aleyrodidarum, and the secondary symbionts Hamiltonella, Cardinium and Wolbachia, a Rickettsia sp. was detected for the first time in this insect family. The distribution, transmission, and localization of the Rickettsia were studied using PCR and fluorescence in situ hybridization (FISH). Rickettsia was found in all 20 Israeli B. tabaci populations screened as well as some populations screened in the Arizona laboratory, but not in all individuals within each population. FISH analysis of B. tabaci eggs, nymphs and adults, revealed a unique concentration of Rickettsia around the gut and follicle cells as well as its random distribution in the haemolymph, but absence from the primary symbiont housing cells, the bacteriocytes. Rickettsia vertical transmission on the one hand and its partial within-population infection on the other suggest a phenotype that is advantageous under certain conditions but may be deleterious enough to prevent fixation under others.To test for the possible involvement of Wolbachia and Cardiniumin the reproductive isolation of different B. tabacibiotypes, reciprocal crosses were preformed among populations of the Cardinium-infected, Wolbachia-infected and uninfected populations. The crosses results demonstrated that phylogeographically divergent B. tabaci are reproductively competent and that cytoplasmic incompatibility inducer-bacteria (Wolbachia and Cardinium) both interfered with, and/or rescued CI induced by one another, effectively facilitating bidirectional female offspring production in the latter scenario.This knowledge has implications to multitrophic interactions, gene flow, speciation, fitness, natural enemy interactions, and possibly, host preference and virus transmission. Although extensive and creative attempts undertaken in both laboratories to cure whiteflies of non-primary symbionts have failed, our finding of naturally uninfected individuals have permitted the establishment of Rickettsia-, Wolbachia- and Cardinium-freeB. tabaci lines, which are been employed to address various biological questions, including determining the role of these bacteria in whitefly host biology.
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Kadouri, Daniel E. The Potential Application and Risks Associated With the Use of Predatory Bacteria as a Bio-control Agent Against Wound Infections. Fort Belvoir, VA : Defense Technical Information Center, octobre 2015. http://dx.doi.org/10.21236/ada625508.

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Ciapponi, Agustín. What is the effectiveness of interventions to improve antibiotic prescribing practices for hospital inpatients ? SUPPORT, 2016. http://dx.doi.org/10.30846/1610132.

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In-hospital infections caused by antibiotic-resistant bacteria and Clostridium difficile are associated with higher rates of death, illness and prolonged hospital stay which is a serious problem for patients and healthcare systems. These infections occur because antibiotics are used too often and incorrectly.
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Lighthart, Bruce, B. T. Shaffer, A. S. Frisch et D. Paterno. Meteorological Variables Associated with Population Density of Culturable Atmospheric Bacteria at a Summer Site in the Mid-Willamette River Valley, Oregon. Fort Belvoir, VA : Defense Technical Information Center, septembre 2004. http://dx.doi.org/10.21236/ada427789.

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