Articles de revues : « AS-LAMP »

1

Imam, Husain. « Broad as a lamp, bright as a laser ». Nature Photonics 2, n^o 1 (janvier 2008) : 26–28. http://dx.doi.org/10.1038/nphoton.2007.270.

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Hua, Chi T., John J. Hopwood, Sven R. Carlsson, Ray J. Harris et Peter J. Meikle. « Evaluation of the lysosome-associated membrane protein LAMP-2 as a marker for lysosomal storage disorders ». Clinical Chemistry 44, n^o 10 (1 octobre 1998) : 2094–102. http://dx.doi.org/10.1093/clinchem/44.10.2094.

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Abstract For many lysosomal storage disorders, presymptomatic detection, before the onset of irreversible pathology, will greatly improve the efficacy of current and proposed therapies. In the absence of a family history, presymptomatic detection can be achieved only by a comprehensive newborn screening program. Recently we reported that the lysosome-associated membrane protein LAMP-1 was increased in the plasma from ∼70% of individuals with lysosomal storage disorders. Here we report on the evaluation of a second lysosome-associated membrane protein, LAMP-2, as a marker for this group of disorders. The median concentration of LAMP-2 in the plasma of healthy individuals was 1.21 mg/L, fourfold higher than the median LAMP-1 concentration (0.31 mg/L). LAMP-2 was increased in >66% of patients with lysosomal storage disorders, and the increases coincided with increased LAMP-1 concentrations. The reference intervals for LAMP-1 and LAMP-2 in blood spots taken from newborns were 0.20–0.54 mg/L (n = 1600) and 0.95–3.06 mg/L (n = 1600), respectively. A high correlation was observed between the concentrations of LAMP-1 and LAMP-2 in both control and affected individuals. The higher concentrations of LAMP-2, relative to LAMP-1, in plasma make LAMP-2 an attractive marker; however, the final selection will be dependent on the availability of new diagnostic markers and their ability to detect disorders currently not identified by LAMP-2.

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Ko, Jae-jun, et Chung-hyeok Kim. « A Study on the Optical Characteristics of LED Lamp as Alternative Type of FPL Lamp ». Journal of the Korean Institute of Illuminating and Electrical Installation Engineers 29, n^o 10 (30 octobre 2015) : 1–6. http://dx.doi.org/10.5207/jieie.2015.29.10.001.

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Ma, C. K., et R. E. Bedford. « Lamp Resistance as a Criterion for Assessing Thermal Equilibrium of the Tungsten Strip-filament Lamp ». Metrologia 27, n^o 3 (1 janvier 1990) : 153–55. http://dx.doi.org/10.1088/0026-1394/27/3/007.

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CHANG, Melissa H. Y., Chi T. HUA, Elizabeth L. ISAAC, Tom LITJENS, Greg HODGE, Litsa E. KARAGEORGOS et Peter J. MEIKLE. « Transthyretin interacts with the lysosome-associated membrane protein (LAMP-1) in circulation ». Biochemical Journal 382, n^o 2 (24 août 2004) : 481–89. http://dx.doi.org/10.1042/bj20031752.

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LAMP-1 (lysosome-associated membrane protein), a major glycoprotein present in the lysosomal membrane, constitutes up to 50% of total membrane proteins. LAMP-1, expressed at the plasma membrane, is reported to be the major molecule expressing the sialyl-Lewis X antigen. Two forms of LAMP-1 exist; the full-length LAMP-1 [LAMP-1 (+Tail)] has a highly glycosylated lumenal domain, a membrane-spanning domain and a short cytoplasmic tail, and the truncated LAMP-1 [LAMP-1 (−Tail)] contains only the lumenal domain. Soluble LAMP-1 (±Tail) has been reported in circulation. LAMP-1 at the cell surface has been shown to interact with E-selectin and galectin and is proposed to function in cell–cell interactions. However, the functional role(s) of soluble LAMP-1 in circulation is unclear. To investigate the functional role of soluble LAMP-1 in circulation, recombinant LAMP-1 (−Tail) and LAMP-1 (+Tail) were produced in HT1080 cells. Two immune-quantification assays were developed to distinguish between the LAMP-1 forms. The interaction and aggregation properties of the different LAMP-1 forms were investigated using the immune-quantification assays. Only LAMP-1 (+Tail) was found to aggregate and interact with plasma proteins. Plasma proteins that interact with LAMP-1 were isolated by affinity chromatography with either the recombinant LAMP-1 (−Tail) or a synthesized peptide consisting of the 14 amino acids of the LAMP-1 cytoplasmic tail. Transthyretin was found to interact with the cytoplasmic tail of LAMP-1. Transthyretin exists as a homotetramer in plasma, as such may play a role in the aggregation of LAMP-1 in circulation.

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Chang, TaC, et Matteo Ziff. « Using smartphone flashlight as slit lamp light source ». Indian Journal of Ophthalmology 68, n^o 8 (2020) : 1658. http://dx.doi.org/10.4103/ijo.ijo_2335_19.

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Sung, Wen-Tsai, Ihzany Vilia Devi et Sung-Jung Hsiao. « Smart Lamp Using Google Firebase as Realtime Database ». Intelligent Automation & ; Soft Computing 33, n^o 2 (2022) : 967–82. http://dx.doi.org/10.32604/iasc.2022.024664.

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Schulman, M. B., et D. R. Woodward. « Plasma‐enhanced photoemission as a discharge lamp diagnostic ». Applied Physics Letters 55, n^o 16 (16 octobre 1989) : 1618–20. http://dx.doi.org/10.1063/1.102216.

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Mohd Khairudin, Rahman, M. I. Mohd Hafzi et Hamzan Azhar. « Amber Position Lamp as Daytime Running Light for Motorcycle ». Advanced Engineering Forum 10 (décembre 2013) : 357–60. http://dx.doi.org/10.4028/www.scientific.net/aef.10.357.

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The ability for motorcycle to be detected is an important aspect in preventing crash involving motorcycle which is the most dominant vehicle in emerging countries. Widely referred as conspicuity, the crash factor is appropriately addressed by the introduction of mandatory daytime running light (DRL) law and is usually a success story in many parts of the world. In 2011, there was a motion introduced in the 64thsession of the United Nations Working Party on Lighting and Light-Signalling (GRE) for amber position lamp (APL) to be made mandatory on motorcycle as additional measure to improve motorcycle conspicuity. An experiment was designed to evaluate conspicuity level of motorcycle headlamp and tail lamp equipped with APL over motorcycle with present DRL setting (baseline). 15 participants simultaneously rated both motorcycles which are placed in parallel, at different distances and times of day. Motorcycle with APL was noticeably better detected from rear than front at 50 meter and 100 meter distance, as well as during night time and twilight. Median conspicuity level between night time and daytime and between night time and twilight was also distinctly different for rear lamp. These findings suggest that APL introduction could enhance motorcycle conspicuity especially for rear lamp position.

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Silverstein, RL, et M. Febbraio. « Identification of lysosome-associated membrane protein-2 as an activation-dependent platelet surface glycoprotein ». Blood 80, n^o 6 (15 septembre 1992) : 1470–75. http://dx.doi.org/10.1182/blood.v80.6.1470.1470.

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Abstract Platelets undergo biochemical and morphologic changes when stimulated that greatly alter their function and contribute to their role in thrombosis and hemostasis. We recently identified and cloned the cDNA for a platelet surface glycoprotein expressed on activated, not resting cells. We found that this protein, lysome-associated membrane protein-1 (LAMP-1), is an integral membrane protein of the lysosome that translocated to the surface membrane when platelets were stimulated by a strong agonist. We now show with immunofluorescence flow cytometry that LAMP-2, a lysosomal membrane protein that shares approximately 30% homology with LAMP-1, is also expressed preferentially on the surface of activated platelets. Equilibrium binding studies with 125I-anti-LAMP- 2 IgG showed approximately 1,100 binding sites per thrombin-stimulated platelet and less than 50 per resting platelet. Sucrose gradient ultracentrifugation fractionation of resting platelet sonicates showed that LAMP-2 colocalized with LAMP-1 and with lysosomal enzymes, and not with thrombospondin or serotonin, which are markers of the two other platelet granule compartments, alpha-granules and dense granules. LAMP- 2 surface expression was minimal in response to platelet stimulation by weak agonists such as epinephrine and ADP. These data show that LAMP-2, like LAMP-1, translocates from the lysosomal membrane compartment to the surface membrane when platelets are activated. Regulated surface expression of these heavily glycosylated proteins may play a role in the adhesive, prothrombotic phenotype of these cells.

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Silverstein, RL, et M. Febbraio. « Identification of lysosome-associated membrane protein-2 as an activation-dependent platelet surface glycoprotein ». Blood 80, n^o 6 (15 septembre 1992) : 1470–75. http://dx.doi.org/10.1182/blood.v80.6.1470.bloodjournal8061470.

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Platelets undergo biochemical and morphologic changes when stimulated that greatly alter their function and contribute to their role in thrombosis and hemostasis. We recently identified and cloned the cDNA for a platelet surface glycoprotein expressed on activated, not resting cells. We found that this protein, lysome-associated membrane protein-1 (LAMP-1), is an integral membrane protein of the lysosome that translocated to the surface membrane when platelets were stimulated by a strong agonist. We now show with immunofluorescence flow cytometry that LAMP-2, a lysosomal membrane protein that shares approximately 30% homology with LAMP-1, is also expressed preferentially on the surface of activated platelets. Equilibrium binding studies with 125I-anti-LAMP- 2 IgG showed approximately 1,100 binding sites per thrombin-stimulated platelet and less than 50 per resting platelet. Sucrose gradient ultracentrifugation fractionation of resting platelet sonicates showed that LAMP-2 colocalized with LAMP-1 and with lysosomal enzymes, and not with thrombospondin or serotonin, which are markers of the two other platelet granule compartments, alpha-granules and dense granules. LAMP- 2 surface expression was minimal in response to platelet stimulation by weak agonists such as epinephrine and ADP. These data show that LAMP-2, like LAMP-1, translocates from the lysosomal membrane compartment to the surface membrane when platelets are activated. Regulated surface expression of these heavily glycosylated proteins may play a role in the adhesive, prothrombotic phenotype of these cells.

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12

Tanabe, N., K. Go, Y. Sakurada, M. Imasawa, F. Mabuchi, T. Chiba, K. Abe et K. Kashiwagi. « A Remote Operating Slit Lamp Microscope System ». Methods of Information in Medicine 50, n^o 05 (2011) : 427–34. http://dx.doi.org/10.3414/me10-01-0064.

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SummaryObjectives: To develop a remote-operating slit lamp microscope system (the remote slit lamp) as the core for highly specialized ophthalmology diagnoses, and to compare the utility of this system with the conventional slit lamp microscope system (the conventional slit lamp) in making a diagnosis.Methods: The remote slit lamp system was developed. Three factors were evaluated in comparison to the conventional slit lamp. The ability to acquire skills was investigated using a task loading system among specialists and residents in ophthalmology. Participants repeated a task up to ten times and the time required for each task was analyzed. The consistency of the two systems in making a diagnosis was investigated using eyes of patients with ocular diseases as well as healthy volunteers.Results: The remote slit lamp is composed of a patient’s unit and ophthalmologist’s unit connected by high-speed internet. The two units share images acquired by the slit lamp in addition to the images and voices of patients and ophthalmologists. Both ophthalmology specialists and residents could minimize the completion times after several trials. The remote slit lamp took more time than the conventional slit lamp. Both systems showed a high consistency in evaluations among eyes with healthy eyes or those with ocular diseases.Conclusions: The remote slit lamp has a similar diagnostic ability, but required more examination time in comparison to the conventional slit lamp. The currently developed remote slit lamp has the potential to be employed for telemedicine purposes in the field of ophthalmology.

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Ravindran, Aravind, Julien Levy, Elizabeth Pierson et Dennis C. Gross. « Development of a Loop-Mediated Isothermal Amplification Procedure as a Sensitive and Rapid Method for Detection of ‘Candidatus Liberibacter solanacearum’ in Potatoes and Psyllids ». Phytopathology® 102, n^o 9 (septembre 2012) : 899–907. http://dx.doi.org/10.1094/phyto-03-12-0055-r.

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This study reports the development of a loop-mediated isothermal amplification procedure (LAMP) for polymerase chain reaction (PCR)-based detection of ‘Candidatus Liberibacter solanacearum’, the bacterial causal agent of potato zebra chip (ZC) disease. The 16S rDNA gene of ‘Ca. Liberibacter solanacearum’ was used to design a set of six primers for LAMP PCR detection of the bacterial pathogen in potato plants and the psyllid vector. The advantage of the LAMP method is that it does not require a thermocycler for amplification or agarose gel electrophoresis for resolution. Positive LAMP results can be visualized directly as a precipitate. The LAMP strategy reported here reliably detected ‘Ca. Liberibacter solanacearum’ and the closely related species ‘Ca. Liberibacter asiaticus’, the causative agent of huanglongbing disease of citrus, in plant DNA extracts. Although not as sensitive as quantitative real-time PCR, LAMP detection was equivalent to conventional PCR in tests of ZC-infected potato plants from the field. Thus, the LAMP method shows strong promise as a reliable, rapid, and cost-effective method of detecting ‘Ca. Liberibacter’ pathogens in psyllids and field-grown potato plants and tubers.

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Saito, Yuichi, Atsuka Matsui, Satoru Michiyuki, Hiroaki Morooka, Takayuki Ibi, Yoshikane Yamauchi, Nobumasa Takahashi et al. « Loop-Mediated Isothermal Amplification as Point-of-Care Testing for EGFR-Mutated Lung Adenocarcinoma ». Micromachines 13, n^o 6 (6 juin 2022) : 897. http://dx.doi.org/10.3390/mi13060897.

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Liquid biopsy has been adapted as a diagnostic test for EGFR mutations in patients with advanced or metastatic non-small cell lung cancer (NSCLC). Loop-mediated isothermal amplification (LAMP) has been widely used for the rapid detection of pathogens through DNA amplification. This study investigated the efficacy of an EGFR-LAMP assay using plasma samples of patients with resected NSCLC tumors. The EGFR status was investigated using both LAMP and next-generation sequencing (NGS) assays in cases that met the following criteria: (1) pulmonary adenocarcinoma with EGFR mutation detected by the Therascreen EGFR PCR Kit and (2) preoperative plasma samples contained enough DNA for the LAMP and NGS experiments. Among 51 specimens from patients with EGFR-mutated tumors or metastatic lymph nodes, the LAMP assay detected 1 EGFR mutation that was also detected in the NGS assay. However, a plasma sample that demonstrated EGFR wild type in the LAMP assay showed an EGFR mutant status in NGS. The detection rates (1.9% in LAMP and 3.9% in NGS) were very low in both assays, demonstrating a similar performance in detecting EGFR mutations in NSCLC tumors; therefore, it could be a more suitable test for the advanced stage, not the early stage. Notably, the LAMP assay was more time-saving, cost-effective, and straightforward. However, further investigation is required to develop a more sensitive assay.

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Arbaciauskaite, Skaiste, Pouya Babakhani, Natalia Sandetskaya, Dalius Vitkus, Ligita Jancoriene, Dovile Karosiene, Dovile Karciauskaite, Birute Zablockiene et Dirk Kuhlmeier. « Self-Sampled Gargle Water Direct RT-LAMP as a Screening Method for the Detection of SARS-CoV-2 Infections ». Diagnostics 12, n^o 4 (22 mars 2022) : 775. http://dx.doi.org/10.3390/diagnostics12040775.

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We assessed the viability of self-sampled gargle water direct RT-LAMP (LAMP) for detecting SARS-CoV-2 infections by estimating its sensitivity with respect to the gold standard indirect RT-PCR of paired oro-nasopharyngeal swab samples. We also assessed the impact of symptom onset to test time (STT)—i.e., symptom days at sampling, on LAMP. In addition, we appraised the viability of gargle water self-sampling versus oro-nasopharyngeal swab sampling, by comparing paired indirect RT-PCR results. 202 oro-nasopharyngeal swab and paired self-sampled gargle water samples were collected from hospital patients with COVID-19 associated symptoms. LAMP, indirect and direct RT-PCR were performed on all gargle water samples, and indirect RT-PCR was performed on all oro-nasopharyngeal samples. LAMP presented a sensitivity of 80.8% (95% CI: 70.8–90.8%) for sample pairs with sub-25 Ct oro-nasopharyngeal indirect RT-PCR results, and 77.6% (66.2–89.1%) sensitivity for sub-30 Ct samples with STT ≤ 7 days. STT, independently of Ct value, correlated negatively with LAMP performance. 80.7% agreement was observed between gargle water and oro-nasopharyngeal indirect RT-PCR results. In conclusion, LAMP presents an acceptable sensitivity for low Ct and low STT samples. Gargle water may be considered as a viable sampling method, and LAMP as a screening method, especially for symptomatic persons with low STT values.

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Lee, Pui-Yuei, Yien-Ping Wong, Shuhaidah Othman et Hui-Yee Chee. « Room-temperature stable loop-mediated isothermal amplification (LAMP) reagents to detect leptospiral DNA ». Asian Biomedicine 15, n^o 4 (1 août 2021) : 183–89. http://dx.doi.org/10.2478/abm-2021-0023.

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Abstract Background Loop-mediated isothermal amplification (LAMP) is one of the most promising tools for rapidly detecting Leptospira spp. However, LAMP is hampered by cold storage to maintain the enzymatic activity of Bst DNA polymerase. Objective To overcome the drawback of cold storage requirement for LAMP reagents we modified the reagents by adding sucrose as stabilizer. We then sought to determine the stability at room temperature of the premixed LAMP reagents containing sucrose. Method Premixed LAMP reagents with sucrose and without sucrose were prepared. The prepared mixtures were stored at room temperature for up to 60 days, and were subjected to LAMP reactions at various intervals using rat kidney samples to detect leptospiral DNA. Results The premixed LAMP reagents with sucrose remained stable for 45 days while sucrose-free premixed LAMP reagents showed no amplification from day 1 of storage at room temperature up to day 14. Conclusion The LAMP reagent system can be refined by using sucrose as stabilizer, thus allowing their storage at room temperature without the need for cold storage. The modified method enables greater feasibility of LAMP for field surveillance and epidemiology in resource-limited settings.

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Netongo, Palmer Masumbe, Severin Donald Kamdem, Ardin Noutong Ouayoue, Theophile Patrick Djiokeng, Sophie Wankio Toju, Eric Berenger Tchoupe, Prisca Djivida, Jean Paul Chedjou, Irenée Domkam et Wilfred Mbacham. « Noninvasive Approach of Plasmodium falciparum Molecular Detection for Malaria Surveillance in Malaria Endemic Areas in Cameroon ». BioMed Research International 2022 (9 novembre 2022) : 1–8. http://dx.doi.org/10.1155/2022/3600354.

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Background. Accurate, cost-effective, and noninvasive alternative molecular methods are needed for detecting low malaria parasitemia. The currently-used nested polymerase chain reaction (nPCR) requires blood as well as skilled personnel in order to minimise the risk of bloodborne disease transmission. Therefore, this study is aimed at assessing the accuracy of a noninvasive and more affordable malaria diagnosis with saliva using the loop-mediated isothermal amplification (LAMP) technique. Methods. A cross-sectional study was conducted in the Centre and Southwest regions of Cameroon. Matched blood and saliva samples collected from symptomatic and asymptomatic participants were tested for malaria using rapid diagnostic tests, microscopy, PCR, and LAMP. Statistics were performed using R studio software at 95% confidence interval. Results. A total of 100 participants (65% symptomatic and 35% asymptomatic) aged between 1 and 74 years with a balanced gender distribution ratio of 1.08 were included in our study. The prevalence of malaria was 61%, 57%, 59%, 42%, 35%, 17%, and 16% for blood-RDT, blood-PCR, blood-LAMP, blood-RT-LAMP, saliva-PCR, saliva-RT-LAMP, and saliva-LAMP, respectively. Both saliva and blood showed a sensitivity of 43.90% and respective specificities of 68.75% and 57.62%. When using RT-LAMP, sensitivities of 49.38% and 48.21% and specificities of 94.11% and 66.67% were recorded for saliva and blood, respectively. Sensitivities of 70.23% and 73.49% and specificities of 62.5% and 76.47% were recorded, respectively, for saliva-LAMP and saliva-RT-LAMP when compared to saliva-PCR as the gold standard. Saliva-LAMP and saliva-RT-LAMP had a fair agreement ( к = 0.221 and 0.352, respectively) with saliva-PCR. Homemade LAMP and RT-LAMP technologies match the WHO recommendations and after proper validation in a larger sample size, could serve for malaria diagnosis in developing countries.

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Yanagihara, Masahiro, Takayuki Tsuji, Mohd Zamri Yusop, Masaki Tanemura, Shingo Ono, Tomohito Nagami, Kentaro Fukuda et al. « Vacuum Ultraviolet Field Emission Lamp Consisting of Neodymium Ion Doped Lutetium Fluoride Thin Film as Phosphor ». Scientific World Journal 2014 (2014) : 1–5. http://dx.doi.org/10.1155/2014/309091.

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A vacuum ultraviolet (VUV) field emission lamp was developed by using a neodymium ion doped lutetium fluoride (Nd3+ : LuF3) thin film as solid-state phosphor and carbon nanofiber field electron emitters. The thin film was synthesized by pulsed laser deposition and incorporated into the lamp. The cathodoluminescence spectra of the lamp showed multiple emission peaks at 180, 225, and 255 nm. These emission spectra were in good agreement with the spectra reported for the Nd3+ : LuF3crystal. Moreover, application of an acceleration voltage effectively increased the emission intensity. These results contribute to the performance enhancement of the lamp operating in the VUV region.

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Rhee, S. W., H. S. Park et H. H. Choi. « Comparison Of Mercury Distribution Between The Types Of Spent Fluorescent Lamp ». Archives of Metallurgy and Materials 60, n^o 2 (1 juin 2015) : 1297–99. http://dx.doi.org/10.1515/amm-2015-0117.

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Abstract Spent fluorescent lamps(SFLs) such as linear type lamp, compact type lamp and U-type lamp are used to estimate mercury distribution in the components of lamps. Determination of mercury concentration in the components of spent fluorescent lamp is performed by the DMA method. Mercury concentration in the components of spent fluorescent lamp can be varied with the manufactures of lamp. Mercury portion in phosphor powder and glass from any types of spent fluorescent lamp is estimated to be higher than 99% by the analysis of mercury distribution. Through mercury distribution in the components for SFLs, the mercury concentration in phosphor powder is much higher than that in other components regardless of the type of lamp. Hence, it is desirable that phosphor powder of spent fluorescent lamps should be controlled separately and safely.

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Pérez, Liliana, Sarah Deffit et Janice Blum. « LAMP-2C regulation of MHC class II antigen presentation (APP3P.105) ». Journal of Immunology 192, n^o 1_Supplement (1 mai 2014) : 111.6. http://dx.doi.org/10.4049/jimmunol.192.supp.111.6.

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Abstract In autophagy cells divert cytoplasmic components and organelles to lysosomes for degradation impacting innate and adaptive immunity. A selective form of autophagy, known as chaperone-mediated autophagy (CMA), is implicated in adaptive immunity. CMA relies on the lysosome-associated membrane protein (LAMP)-2A to translocate proteins from the cytoplasm into lysosomes. Alternative splicing of the LAMP-2 gene generates 3 highly conserved isoforms LAMP-2A, LAMP-2B and LAMP-2C. The function and regulation of these genes is not well understood. Here we identified a novel role for LAMP-2C in the regulation of CMA. Studies were undertaken here to understand the role of LAMP-2C in MHC class II presentation of cytoplasmic antigens. Ectopic expression of LAMP-2C did not alter the maturation or the enzymatic activity of lysosomal enzymes. Furthermore, LAMP-2C ectopic expression failed to perturb other degradation pathways involved in antigen presentation like macroautophagy and proteasome degradation. Thus, LAMP-2C appears to have a selective regulatory role with respect to the presentation of cytoplasmic proteins. Future studies are underway to determine mechanistically how LAMP-2C controls antigen presentation. This work was supported by NIH 5T32AI060519 and AI079065.

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Sohrabi, Amir, Joar Franzen, Nikolaos Tertipis, Ulrika Zagai, Wanxin Li, Zongli Zheng et Weimin Ye. « Efficacy of Loop-Mediated Isothermal Amplification for H. pylori Detection as Point-of-Care Testing by Noninvasive Sampling ». Diagnostics 11, n^o 9 (25 août 2021) : 1538. http://dx.doi.org/10.3390/diagnostics11091538.

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For targeted eradication of Helicobacter pylori (H. pylori) to reduce gastric cancer burden, a convenient approach is definitely needed. The purpose of this study was to evaluate the LAMP assay for H. pylori detection using samples collected by noninvasive and self-sampling methods. The available LAMP assay for H. pylori detection was appraised and verified using reference and clinically isolated H. pylori strains. In addition, a clinical study was conducted to assess the LAMP assay on 51 patients, from whom saliva, oral brushing samples, feces, corpus, and antrum specimens were available. Clarithromycin resistance was also analysed through detection of A2143G mutation using the LAMP-RFLP method. The validation and verification analysis demonstrated that the LAMP assay had an acceptable result in terms of specificity, sensitivity, reproducibility, and accuracy for clinical settings. The LAMP assay showed a detection limit for H. pylori down to 0.25 fg/µL of genomic DNA. An acceptable consensus was observed using saliva samples (sensitivity 58.1%, specificity 84.2%, PPV 85.7%, NPV 55.2%, accuracy 68%) in comparison to biopsy sampling as the gold standard. The performance testing of different combinations of noninvasive sampling methods demonstrated that a combination of saliva and oral brushing could achieve a sensitivity of 74.2% and a specificity of 57.9%. A2143G mutation detection by LAMP-RFLP showed perfect consensus with Sanger sequencing results. It appears that the LAMP assay in combination with noninvasive and self-sampling as a point-of-care testing (POCT) approach has potential usefulness to detect H. pylori infection in clinic settings and screening programs.

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Li, Tongzhe, et Hongkui Zhang. « Design of explosion-proof induction lamp temperature test system ». Highlights in Science, Engineering and Technology 27 (27 décembre 2022) : 691–95. http://dx.doi.org/10.54097/hset.v27i.3833.

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Temperature test is an item to be tested in the type test of explosion-proof induction lamp. Temperature test is an item to be tested in the type test of explosion-proof induction lamp. A temperature test system based on single chip microcomputer integrated control and automatic trigger is proposed to improve the efficiency and stability of temperature test of explosion-proof induction lamp. Based on the analysis of the working principle of the explosion-proof induction lamp, the explosion-proof induction lamp temperature test system is designed, and the full-power trigger module of the explosion-proof induction lamp is designed in detail, so as to realize the automatic operation of the explosion-proof induction lamp temperature test.

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Ku, Jamin, Khushbu Chauhan, Sang-Hyun Hwang, Yong-Joo Jeong et Dong-Eun Kim. « Enhanced Specificity in Loop-Mediated Isothermal Amplification with Poly(ethylene glycol)-Engrafted Graphene Oxide for Detection of Viral Genes ». Biosensors 12, n^o 8 (20 août 2022) : 661. http://dx.doi.org/10.3390/bios12080661.

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Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method that allows the simple, quick, and low-cost detection of various viral genes. LAMP assays are susceptible to generating non-specific amplicons, as high concentrations of DNA primers can give rise to primer dimerization and mismatched hybridizations, resulting in false-positive signals. Herein, we reported that poly(ethylene glycol)-engrafted nanosized graphene oxide (PEG-nGO) can significantly enhance the specificity of LAMP, owing to its ability to adsorb single-stranded DNA (ssDNA). By adsorbing surplus ssDNA primers, PEG-nGO minimizes the non-specific annealing of ssDNAs, including erroneous priming and primer dimerization, leading to the enhanced specificity of LAMP. The detection of complementary DNAs transcribed from the hepatitis C virus (HCV) RNA was performed by the PEG-nGO-based LAMP. We observed that the inclusion of PEG-nGO significantly enhances the specificity and sensitivity of the LAMP assay through the augmented difference in fluorescence signals between the target and non-target samples. The PEG-nGO-based LAMP assay greatly facilitates the detection of HCV-positive clinical samples, with superior precision to the conventional quantitative real-time PCR (RT-qPCR). Among the 20 clinical samples tested, all 10 HCV-positive samples are detected as positive in the PEG-nGO-based LAMP, while only 7 samples are detected as HCV-positive in the RT-qPCR. In addition, the PEG-nGO-based LAMP method significantly improves the detection precision for the false-positive decision by 1.75-fold as compared to the LAMP without PEG-nGO. Thus, PEG-nGO can significantly improve the performance of LAMP assays by facilitating the specific amplification of target DNA with a decrease in background signal.

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Mazina, S. E. « BRYOPHYTES AND FERNS AS PART OF LAMP FLORA CAVES ». South of Russia : ecology, development 11, n^o 3 (1 janvier 2016) : 140–50. http://dx.doi.org/10.18470/1992-1098-2016-3-140-150.

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SIVAK, MICHAEL, et MICHAEL FLANNAGAN. « Fast–rise brake lamp as a collision–prevention device ». Ergonomics 36, n^o 4 (avril 1993) : 391–95. http://dx.doi.org/10.1080/00140139308967896.

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Ferrero, Francisco, Manuel Rico Secades, Cecilio Blanco, Pérez Miguel, Juan Campo, Manuela Vega et Juan Antón. « Resonant Converter as a High Pressure Sodium Lamp Ballast ». EPE Journal 9, n^o 3-4 (janvier 2000) : 33–40. http://dx.doi.org/10.1080/09398368.2000.11463448.

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Suster, Albert L. « Stress Corrosion Resistant Alloys as Materials for Lamp Bases ». Journal of the Illuminating Engineering Society 19, n^o 1 (janvier 1990) : 52–56. http://dx.doi.org/10.1080/00994480.1990.10747940.

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Mannier, Cassidy, et Jeong-Yeol Yoon. « Progression of LAMP as a Result of the COVID-19 Pandemic : Is PCR Finally Rivaled ? » Biosensors 12, n^o 7 (6 juillet 2022) : 492. http://dx.doi.org/10.3390/bios12070492.

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Reflecting on the past three years and the coronavirus disease 19 (COVID-19) pandemic, varying global tactics offer insights into the most effective public-health responses. In the US, specifically, rapid and widespread testing was quickly prioritized to lower restrictions sooner. Essentially, only two types of COVID-19 diagnostic tests were publicly employed during the peak pandemic: the rapid antigen test and reverse transcription polymerase chain reaction (RT-PCR). However, neither test ideally suited the situation, as rapid antigen tests are far too inaccurate, and RT-PCR tests require skilled personnel and sophisticated equipment, leading to long wait times. Loop-mediated isothermal amplification (LAMP) is another exceptionally accurate nucleic acid amplification test (NAAT) that offers far quicker time to results. However, RT-LAMP COVID-19 tests have not been embraced as extensively as rapid antigen tests or RT-PCR. This review will investigate the performance of current RT-LAMP-based COVID-19 tests and summarize the reasons behind the hesitancy to embrace RT-LAMP instead of RT-PCR. We will also look at other LAMP platforms to explore possible improvements in the accuracy and portability of LAMP, which could be applied to COVID-19 diagnostics and future public-health outbreaks.

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Chi, Congwu, Andrea Leonard, Walter E. Knight, Kevin M. Beussman, Yuanbiao Zhao, Yingqiong Cao, Pilar Londono et al. « LAMP-2B regulates human cardiomyocyte function by mediating autophagosome–lysosome fusion ». Proceedings of the National Academy of Sciences 116, n^o 2 (24 décembre 2018) : 556–65. http://dx.doi.org/10.1073/pnas.1808618116.

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Mutations in lysosomal-associated membrane protein 2 (LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart failure through poorly defined mechanisms. Here, we identify the LAMP-2 isoform B (LAMP-2B) as required for autophagosome–lysosome fusion in human cardiomyocytes (CMs). Remarkably, LAMP-2B functions independently of syntaxin 17 (STX17), a protein that is essential for autophagosome–lysosome fusion in non-CMs. Instead, LAMP-2B interacts with autophagy related 14 (ATG14) and vesicle-associated membrane protein 8 (VAMP8) through its C-terminal coiled coil domain (CCD) to promote autophagic fusion. CMs derived from induced pluripotent stem cells (hiPSC-CMs) from Danon patients exhibit decreased colocalization between ATG14 and VAMP8, profound defects in autophagic fusion, as well as mitochondrial and contractile abnormalities. This phenotype was recapitulated by LAMP-2B knockout in non-Danon hiPSC-CMs. Finally, gene correction of LAMP-2 mutation rescues the Danon phenotype. These findings reveal a STX17-independent autophagic fusion mechanism in human CMs, providing an explanation for cardiomyopathy in Danon patients and a foundation for targeting defective LAMP-2B–mediated autophagy to treat this patient population.

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YOKOYAMA, EIJI, MASAKO UCHIMURA et KENITIRO ITO. « Detection of Enteroaggregative Escherichia coli by Loop-Mediated Isothermal Amplification ». Journal of Food Protection 73, n^o 6 (1 juin 2010) : 1064–72. http://dx.doi.org/10.4315/0362-028x-73.6.1064.

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A novel gene amplification method, loop-mediated isothermal amplification (LAMP), has been recently developed as a rapid, specific diagnostic method for various infectious diseases. We have investigated whether LAMP can be used to detect small numbers of enteroaggregative Escherichia coli (EAEC) cells contaminated in food samples. Primers for LAMP reaction were designed with EAEC aggR gene sequences (available in GenBank). LAMP specificity with these primers was the same as that of PCR in a study of 37 EAEC and 42 non-EAEC bacterial strains. The sensitivity of the LAMP method was better than that of PCR in a study of serially diluted EAEC cells. The LAMP method was significantly more effective than was PCR in detecting EAEC-contaminated food samples (Fisher's exact test, P < 0.05). Therefore, the LAMP method described here should be useful for detecting small numbers of EAEC cells in food samples.

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Ko, Jae-jun, et Chung-hyeok Kim. « A study on the safety LED lamp using internal control gear as the alternative type of fluorescent lamp ». Journal of the Korean Institute of Illuminating and Electrical Installation Engineers 29, n^o 3 (31 mars 2015) : 30–38. http://dx.doi.org/10.5207/jieie.2015.29.3.030.

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Murwantoko, Murwantoko. « Metode Loop-Mediated Isothermal Amplification (LAMP) dan Aplikasinya untuk Deteksi Penyakit Ikan ». Jurnal Perikanan Universitas Gadjah Mada 8, n^o 1 (15 février 2006) : 1. http://dx.doi.org/10.22146/jfs.156.

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Polymerase chain reaction (PCR) is a method that amplifies DNA which have been widely used in molecular biology technique. Based on the PCR, many methods have been developed on isothermal condition and the useful one is loop-mediated isothermal amplification of DNA (LAMP). LAMP reaction employs a Bst DNA polymerase and a set of four specific primers that recognizes a total of six distinct sequences of the target DNA and produces amount of different size of DNA. Many advantages have been achieved in LAMP such as the simple equipment for reaction and observation, short time, highly specific and sensitive procedure. LAMP has been used as a tools for detection many pathogens for human, animals and plants. Some fish pathogens as parasites, bacteria and viruses have been detected by LAMP. The principles and application of LAMP method are discussed in this paper.

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Eskelinen, Eeva-Liisa, Christine Katrin Schmidt, Silja Neu, Marion Willenborg, Graciela Fuertes, Natalia Salvador, Yoshitaka Tanaka et al. « Disturbed Cholesterol Traffic but Normal Proteolytic Function in LAMP-1/LAMP-2 Double-deficient Fibroblasts ». Molecular Biology of the Cell 15, n^o 7 (juillet 2004) : 3132–45. http://dx.doi.org/10.1091/mbc.e04-02-0103.

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Mice double deficient in LAMP-1 and -2 were generated. The embryos died between embryonic days 14.5 and 16.5. An accumulation of autophagic vacuoles was detected in many tissues including endothelial cells and Schwann cells. Fibroblast cell lines derived from the double-deficient embryos accumulated autophagic vacuoles and the autophagy protein LC3II after amino acid starvation. Lysosomal vesicles were larger and more peripherally distributed and showed a lower specific density in Percoll gradients in double deficient when compared with control cells. Lysosomal enzyme activities, cathepsin D processing and mannose-6-phosphate receptor expression levels were not affected by the deficiency of both LAMPs. Surprisingly, LAMP-1 and -2 deficiencies did not affect long-lived protein degradation rates, including proteolysis due to chaperone-mediated autophagy. The LAMP-1/2 double-deficient cells and, to a lesser extent, LAMP-2 single-deficient cells showed an accumulation of unesterified cholesterol in endo/lysosomal, rab7, and NPC1 positive compartments as well as reduced amounts of lipid droplets. The cholesterol accumulation in LAMP-1/2 double-deficient cells could be rescued by overexpression of murine LAMP-2a, but not by LAMP-1, highlighting the more prominent role of LAMP-2. Taken together these findings indicate partially overlapping functions for LAMP-1 and -2 in lysosome biogenesis, autophagy, and cholesterol homeostasis.

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Wang, Yin, et Yun Zhe Zhang. « Optimization of Energy-Saving Lighting Line Adjustable Research ». Applied Mechanics and Materials 668-669 (octobre 2014) : 884–87. http://dx.doi.org/10.4028/www.scientific.net/amm.668-669.884.

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Energy-saving lamp will still be a favorite product on the lighting market in the long run. One of the drawbacks of common energy saving lamp is that it cannot be dimmed. Energy is wasted when bright light is not necessary. To promote the idea of energy saving and environmental protection, a plan for the applicability of dimming of energy saving lamp is in urgent need. In this study, we put forward two plans for the dimming of energy saving lamp. Our effort is to make the energy saving lamp adapted to the variety of power frequency and to maximize its smooth dimming as close to that of an incandescent lamp.

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Peng, Huan, Deliang Peng, Xianqi Hu, Xufeng He, Qiong Wang, WenKun Huang et Wenting He. « Loop-mediated isothermal amplification for rapid and precise detection of the burrowing nematode, Radopholus similis, directly from diseased plant tissues ». Nematology 14, n^o 8 (2012) : 977–86. http://dx.doi.org/10.1163/156854112x638415.

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A novel, simple, rapid and highly sensitive assay and diagnostic tool for the burrowing nematode, Radopholus similis, was developed using a loop-mediated isothermal amplification (LAMP). The LAMP assay was targeted on the specific fragments of rRNA gene D2-D3 regions of R. similis. The detection limitation of the LAMP assay was as low as ten copies of plasmid DNA containing the target DNA, 10 fg of genomic DNA and 5 × 10−5 nematodes. The detection sensitivity of the LAMP method for R. similis DNA was 10-100 times higher than normal PCR-based detection methods. The LAMP amplifications could be observed directly by eye by adding SYBR Green I and the lateral flow dipstick (LFD). LAMP assay for R. similis is a practical and useful diagnostic tool for early diagnosis of plant tissues infested by R. similis. The LAMP assay developed in this study is highly effective, easy to perform and readily adaptable for diagnostic and monitoring of the R. similis-diseased seedling in the field.

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Tie, Zhang, Wang Chunguang, Wei Xiaoyuan, Zhao Xinghua et Zhong Xiuhui. « Loop-Mediated Isothermal Amplification for Detection ofStaphylococcus aureusin Dairy Cow Suffering from Mastitis ». Journal of Biomedicine and Biotechnology 2012 (2012) : 1–5. http://dx.doi.org/10.1155/2012/435982.

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To develop a rapid detection method ofStaphylococcus aureususing loop-mediated isothermal amplification (LAMP), four specific primers were designed according to six distinct sequences of thenucgene. In addition, the specificity and sensitivity of LAMP were verified and compared with those of PCR. Results showed that the LAMP reaction was completed within 45 min at 62.5°C, and ladder bands were appeared in LAMP products analyzed by gel electrophoresis. After adding 1x SYBR Green l, the positive reaction tube showed green color and the negative reaction tube remained orange, indicating that the LAMP has high specificity. The minimal detectable concentration of LAMP was1×102 CFU/mL and that of PCR was1×104 CFU/mL, indicating that the LAMP was 100 times more sensitive than the PCR. The LAMP method for detection ofStaphylococcus aureushas many advantages, such as simple operation, high sensitivity, high specificity, and rapid analysis. Therefore, this method is more suitable for the rapid on-site detection ofStaphylococcus aureus.

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Zheng, Youbin, Ping Zhang et Mike Dixon. « Evaluation of Four Lamp Types for the Production of Tomato Plants in Controlled Environments ». HortTechnology 15, n^o 3 (janvier 2005) : 646–52. http://dx.doi.org/10.21273/horttech.15.3.0646.

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To evaluate the performance of four newly developed high-intensity-discharge lamp types on plant growth and production, tomato (Lycopersicon esculentum cv. Tradiro F1) plants were grown indoors under 100% artificial lighting for 17 weeks. The four lamp types were: high-pressure sodium high output [HPS(HO)], high-pressure sodium standard [HPS(STD)], metal halide warm deluxe [MH(WDX)] and metal halide cool deluxe [MH(CDX)]. All the lamps tested were 1000 W. HPS(HO) had the highest electrical energy use efficiency (EUE) (0.98 μmol·m–2·s–1·W–1 at 40 cm directly under the lamp); HPS(STD), MH(WDX) and MH(CDX) had 93%, 72% and 61% of the EUE of the HPS(HO), respectively. The photosynthetically active radiation (PAR) outputs of different lamp types had the following order: HPS(HO) > HPS(STD) > MH(WDX) > MH(CDX). The percentage red of PAR of the four tested lamp types had the same order as above, but the percentage blue of PAR of these lamp types had exactly the opposite order. As a result, plants growing under the two HPS lamp types were taller and flowered and fruited earlier than plants under the two MH lamp types. Chlorophyll content index was generally greater in leaves under MH lamps than in leaves under HPS lamps. We recommend that the HPS lamp be used for flowering and fruiting crops and the MH lamp would be better used for foliar and compact crops.

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Israels, S. J., E. M. McMillan, C. Robertson, S. Singhroy et A. McNicol. « The Lysosomal Granule Membrane Protein, Lamp-2, Is also Present in Platelet Dense Granule Membranes ». Thrombosis and Haemostasis 75, n^o 04 (1996) : 623–29. http://dx.doi.org/10.1055/s-0038-1650333.

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SummaryLysosomal Associated Membrane Protein-2 (LAMP-2) is an inherent component of lysosomal granule membranes in diverse cell types, including platelets. We examined platelets for evidence of LAMP-2 in dense granule membranes as CD63 has previously been shown to be present in both lysosomal and dense granule membranes. Immunological techniques were used to examine the localization of LAMP-2 in control platelets and those from an individual with Hermansky-Pudlak syndrome (HPS), a condition characterised by platelet dense granule deficiency. Immunoblotting studies demonstrated that LAMP-2 was enriched in a dense granule preparation. Flow cytometry of thrombin-stimulated control platelets was consistent with biphasic surface expression of LAMP-2. The early expression was accompanied by dense granule, but minimal lysosomal granule, release. The late expression was accompanied by additional lysosomal granule release only. Thrombin stimulation of HPS platelets showed only late, lysosome-associated LAMP-2 expression. Immunoelectron microscopy indicated the presence of LAMP-2 in the membranes of serotonin-containing granules as identified by an anti-serotonin polyclonal antibody. These data indicate that LAMP-2 is present in the membranes of platelet dense granules in addition to lysosomal granules, and has a similar distribution to CD63.

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Morris, Ulrika, et Berit Aydin-Schmidt. « Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings ». Diagnostics 11, n^o 2 (18 février 2021) : 336. http://dx.doi.org/10.3390/diagnostics11020336.

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Loop-mediated isothermal amplification (LAMP) is a sensitive molecular tool suitable for use as a near point-of-care test for the diagnosis of malaria. Recent meta-analyses have detailed high sensitivity and specificity of malaria LAMP when compared to microscopy, rapid diagnostic tests, and polymerase chain reaction in both endemic and non-endemic settings. Despite this, the use of malaria LAMP has primarily been limited to research settings to date. In this review, we aim to assess to what extent commercially available malaria LAMP kits have been applied in different settings, and to identify possible obstacles that may have hindered their use from being adopted further. In order to address this, we conducted a literature search in PubMed.gov using the search terms (((LAMP) OR (Loop-mediated isothermal amplification)) AND ((Malaria) OR (Plasmodium))). Focusing primarily on studies employing one of the commercially available kits, we then selected three key areas of LAMP application for further review: the performance and application of LAMP in malaria endemic settings including low transmission areas; LAMP for malaria screening during pregnancy; and malaria LAMP in returning travelers in non-endemic settings.

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Zhang, Yinhua, et Nathan A. Tanner. « Improving RT-LAMP detection of SARS-CoV-2 RNA through primer set selection and combination ». PLOS ONE 17, n^o 4 (1 avril 2022) : e0254324. http://dx.doi.org/10.1371/journal.pone.0254324.

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Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has emerged as a viable molecular diagnostic method to expand the breadth and reach of nucleic acid testing, particularly for SARS-CoV-2 detection and surveillance. While rapidly growing in prominence, RT-LAMP remains a relatively new method compared to the standard RT-qPCR, and contribution to our body of knowledge on designing LAMP primer sets and assays can have significant impact on its utility and adoption. Here we select and evaluate 18 LAMP primer sets for SARS-CoV-2 previously identified as sensitive ones under various conditions, comparing their speed and sensitivity with two LAMP formulations each with 2 reaction temperatures. We find that both LAMP formulations have some effects on the speed and detection sensitivity and identify several primer sets with similar high sensitivity for different SARS-CoV-2 gene targets. Significantly we observe a consistent sensitivity enhancement by combining primer sets for different targets, confirming and building on earlier work to create a simple, general approach to building better and more sensitive RT-LAMP assays.

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Hooker, Edmond A., William J. Faulkner, Lisa D. Kelly et Robert C. Whitford. « Prospective study of the sensitivity of the Wood’s lamp for common eye abnormalities ». Emergency Medicine Journal 36, n^o 3 (10 janvier 2019) : 159–62. http://dx.doi.org/10.1136/emermed-2018-208235.

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ObjectiveThe Wood’s lamp, a handheld instrument that uses long-wave ultraviolet (UV) light with magnification of 2–3 times, is commonly used by non-ophthalmologists for examining patients with eye complaints. The goal of current research was to determine the sensitivity and specificity of the Wood’s lamp for common eye abnormalities.Study designWe examined a convenience sample of patients, 18 years of age and older, who presented for eye complaints to an urgent clinic of a large ophthalmology practice. This prospective observational trial was performed from December 2016 until July 2017. An ophthalmologist examined the patient’s eyes with a Wood’s lamp, followed by examination of the eyes using a slit lamp. The Wood’s lamp was compared with the slit lamp, which served as the gold standard.ResultsThere were 73 patients recruited. The mean age of study subjects (29 female and 44 male) was 49 years. The overall sensitivity of the Wood’s lamp was 52% (38/73; 95% CI 40% to 64%). Based on the principal final diagnosis made with the slit lamp, the Wood’s lamp only detected 9 of 16 corneal abrasions, 5 of 10 corneal ulcers, 5 of 9 corneal foreign bodies, 0 of 4 cases of non-herpetic keratitis, 1 of 2 cases of herpes keratitis, 1 of 5 rust rings and 18 of 28 other diagnoses.Conclusions and relevanceExamination using the Wood’s lamp fails to detect many common eye abnormalities. Our findings support the need for a slit lamp examination of patients with eye complaints whenever possible.

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Yoshikawa, Rokusuke, Haruka Abe, Yui Igasaki, Saeki Negishi, Hiroaki Goto et Jiro Yasuda. « Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification ». PLOS Neglected Tropical Diseases 14, n^o 11 (4 novembre 2020) : e0008855. http://dx.doi.org/10.1371/journal.pntd.0008855.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic novel coronavirus that has caused a worldwide outbreak. Here we describe a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that uses a portable device for efficient detection of SARS-CoV-2. This RT-LAMP assay specifically detected SARS-CoV-2 without cross-reacting with the most closely related human coronavirus, SARS-CoV. Clinical evaluation of nasal swab samples from suspected SARS-CoV-2 pneumonia (COVID-19) patients showed that the assay could detect over 23.7 copies within 15 min with a 100% probability. Since the RT-LAMP assay can be performed with a portable battery-supported device, it is a rapid, simple, and sensitive diagnostic assay for COVID-19 that can be available at point-of-care. We also developed the RT-LAMP assay without the RNA extraction step–Direct RT-LAMP, which could detect more than 1.43 x 103 copies within 15 min with a 100% probability in clinical evaluation test. Although the Direct RT-LAMP assay was less sensitive than the standard RT-LAMP, the Direct RT-LAMP assay can be available as the rapid first screening of COVID-19 in poorly equipped areas, such as rural areas in developing countries.

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Mayor-Smith, Ian, et Michael R. Templeton. « Methodological considerations when conducting bench scale polychromatic ultraviolet irradiation of water ». Water Supply 14, n^o 2 (21 octobre 2013) : 291–98. http://dx.doi.org/10.2166/ws.2013.202.

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The use of a bench scale apparatus (often referred to as a ‘collimated beam’) to apply fluences to water samples is common practice in disinfection research and in validating the performance of full-scale UV disinfection reactors. This study investigated the sources of potential experimental variations in the calculation of fluence when conducting polychromatic collimated beam exposures. Spectral variations associated with lamp operating conditions (e.g. cooling of the lamp), the angle of the spectroradiometer relative to the lamp when measuring the UV fluence rate, and the shape of the arc within the lamp are important to consider in order to achieve reproducible UV fluences when using a polychromatic collimated beam. Specific recommendations are provided to encourage greater experimental rigour and reproducibility in polychromatic UV disinfection studies, including taking spectral output measurements before and after UV exposures and monitoring the lamp voltage as an indication of lamp output stability.

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Zhukareva, V., et P. Levitt. « The limbic system-associated membrane protein (LAMP) selectively mediates interactions with specific central neuron populations ». Development 121, n^o 4 (1 avril 1995) : 1161–72. http://dx.doi.org/10.1242/dev.121.4.1161.

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The limbic system-associated membrane protein (LAMP) is a 64–68 × 10(3) M(r) glycoprotein that is expressed by subsets of neurons that are functionally interconnected. LAMP exhibits characteristics that are indicative of a developmentally significant protein, such as an early and restricted pattern of expression and the ability to mediate specific fiber-target interactions. A potential, selective adhesive mechanism by which LAMP may regulate the formation of specific circuits is investigated in the present experiments. LAMP is readily released from intact membranes by phosphatidyl inositol-specific phospholipase C. Purified, native LAMP, isolated by PI-PLC digestion and immunoaffinity chromatography, is capable of mediating fluorescent Covasphere aggregation via homophilic binding. To test the ability of LAMP to selectively facilitate substrate adhesion and growth of neurons from LAMP-positive, in contrast to LAMP-negative regions of the developing brain, purified LAMP was dotted onto nitrocellulose-coated dishes and test cells plated. Limbic neurons from perirhinal cortex bind specifically to substrate-bound LAMP within 4 hours, forming small cell aggregates with short neuritic processes that continue to grow through a 48 hour period of monitoring. Preincubation of cells with anti-LAMP has a modest effect on cell binding but significantly reduces initiation of process growth. Non-limbic neurons from somatosensory cortex and olfactory bulb fail to bind or extend processes on the LAMP substrate to any significant extent. All cell populations bind equally well and form neurites on poly-D-lysine and laminin. The present results provide direct evidence that LAMP can specifically facilitate interactions with select neurons in the CNS during development. The data support the concept that patterned expression of unique cell adhesion molecules in functionally related regions of the mammalian brain can regulate circuit formation.

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Feranisa, Anggun. « KOMPARASI ANTARA POLYMERASE CHAIN REACTION (PCR) DAN LOOPMEDIATED ISOTHERMAL AMPLIFICATION (LAMP) DALAM DIAGNOSIS MOLEKULER ». ODONTO : Dental Journal 3, n^o 2 (1 décembre 2016) : 145. http://dx.doi.org/10.30659/odj.3.2.145-151.

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Background: Molecular diagnostic is an emerging diagnostic method inpersonalized medicine/dentistry era. Usually, it uses nucleic acid amplificationmethod to detect various diseases. PCR is conventional nucleic acid amplification method. However, due to an urgency in infectious diseases’ diagnotic method, scientists developed LAMP as new nucleic acid amplification method.Discussion: There are various experiments used to develop LAMP as infectious diseases diagnostic method compared to PCR. The results are LAMP more sensitive, specific, rapid, and inexpensive than PCR.Conclusion: Both PCR and LAMP can be used as molecular diagnostic tools.LAMP prefer to used as infectious disease diagnostic method in poor anddeveloping countries.

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Guo, Jia Xu, et Ling Long. « The Intelligent Desk Lamp Designed for Special Populations ». Applied Mechanics and Materials 571-572 (juin 2014) : 980–84. http://dx.doi.org/10.4028/www.scientific.net/amm.571-572.980.

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For senior citizens and people with disabilities and other special groups, it is inconvenient for them to use the ordinary desk lamp. This essay presents a design scheme of intelligent desk lamp based on infrared detector. Pyroelectric infrared sensor can detect infrared radiation of human, which can be used as the automatic switch to solve the limitation of the manual switch for these special groups. Intelligent desk lamp can meet these crowds on the specialized requirements of everyday lighting lamp according the characteristic. Compared with the traditional household table lamp, this design has the distinct characteristics of intelligence, pertinence and reliability.

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Boyd, Douglas, et Thomas Guinn. « Efficacy of the Localized Aviation MOS Program in Ceiling Flight Category Forecasts ». Atmosphere 10, n^o 3 (8 mars 2019) : 127. http://dx.doi.org/10.3390/atmos10030127.

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(1) Background: Flying in instrument meteorological conditions (IMC) carries an elevated risk of fatal outcome for general aviation (GA) pilots. For the typical GA flight, aerodrome-specific forecasts (Terminal Aerodrome Forecast (TAF), Localized Aviation Model Output Statistics Program (LAMP)) assist the airman in pre-determining whether a flight can be safely undertaken. While LAMP forecasts are more prevalent at GA-frequented aerodromes, the Federal Aviation Administration (FAA) recommends that this tool be used as supplementary to the TAF only. Herein, the predictive accuracy of LAMP for ceiling flight categories of visual flight rules (VFR) and instrument flight rules (IFR) was determined. (2) Methods: LAMP accuracy was evaluated for the period of July–December 2018 using aviation-specific probability of detection (PODA), false alarm ratio (FARA) and critical success scores (CSSA). Statistical differences were determined using Chi-Square tests. (3) Results: LAMP forecasts (n = 823) across 39 states were accrued. LAMP PODA for VFR (0.67) and IFR (0.78) exceeded (p < 0.031) the corresponding TAF scores (0.57 and 0.56). For VFR, the LAMP showed a non-significant (p = 0.243) higher FARA (0.25) than the TAF (0.19). For IFR forecasts, the LAMP FARA was lower (p < 0.001) (0.48 and 0.81, respectively). LAMP CSSA scores exceeded the TAF for VFR (p = 0.012) and IFR forecasts (p < 0.001). (4) Conclusion: These findings support the greater integration of LAMP into pre-flight weather briefings.

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Arief Budijanto et Tamaji. « Mood Lamp Berbasis Microcontroller ». Jurnal Intake : Jurnal Penelitian Ilmu Teknik dan Terapan 9, n^o 2 (30 octobre 2018) : 53–57. http://dx.doi.org/10.48056/jintake.v9i2.40.

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— Mood lamp is a lamp made from RGB (red, green, blue) led that can be programmed based on its color through remote control, so that the color of the lamp can be determined by the user according to his mood. This lamp is designed using 89C2051 microcontroller with low power technology and uses RGB leds that can be programmed into several colors via a remote control. The remote control signal in this study uses the standard NEC remote code. The results of the led lights experiment can be programmed into 7 colors, namely red, blue, cyan, yellow, magenta, white, and green. The purpose of the results of this study in the future so that it can be used as a business opportunity in the field of creative industries, because this mood lamp can be used as a garden light, home porch lights, restaurants and cafes.

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Tamaji, Arief, et Tamaji Tamaji. « Mood Lamp Berbasis Microcontroller ». Jurnal Intake : Jurnal Penelitian Ilmu Teknik dan Terapan 9, n^o 2 (20 octobre 2018) : 53–57. http://dx.doi.org/10.32492/jintake.v9i2.774.

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Mood lamp is a lamp made from RGB (red, green, blue) led that can be programmed based on its color through remote control, so that the color of the lamp can be determined by the user according to his mood. This lamp is designed using 89C2051 microcontroller with low power technology and uses RGB leds that can be programmed into several colors via a remote control. The remote control signal in this study uses the standard NEC remote code. The results of the led lights experiment can be programmed into 7 colors, namely red, blue, cyan, yellow, magenta, white, and green. The purpose of the results of this study in the future so that it can be used as a business opportunity in the field of creative industries, because this mood lamp can be used as a garden light, home porch lights, restaurants and cafes.

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Mamba, Timothy S., Cecilia K. Mbae, Johnson Kinyua, Erastus Mulinge, Gitonga Nkanata Mburugu et Zablon K. Njiru. « Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers : Detection ofCryptosporidiumSpecies in Kenyan Children Presenting with Diarrhea ». Journal of Tropical Medicine 2018 (2018) : 1–9. http://dx.doi.org/10.1155/2018/7659730.

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Background. Cryptosporidiumis a protozoan parasite and a major cause of diarrhea in children and immunocompromised patients. Current diagnostic methods for cryptosporidiosis such as microscopy have low sensitivity while techniques such as PCR indicate higher sensitivity levels but are seldom used in developing countries due to their associated cost. A loop-mediated isothermal amplification (LAMP) technique, a method with shorter time to result and with equal or higher sensitivity compared to PCR, has been developed and applied in the detection ofCryptosporidiumspecies. The test has a detection limit of 10 pg/µl (~100 oocysts/ml) indicating a need for more sensitive diagnostic tools. This study developed a more sensitive lateral flow dipstick (LFD) LAMP test based on SAM-1 gene and with the addition of a second set of reaction accelerating primers (stem primers).Results. The stem LFD LAMP test showed analytical sensitivity of 10 oocysts/ml compared to 100 oocysts/ml (10 pg/ul) for each of the SAM-1 LAMP test and nested PCR. The stem LFD LAMP and nested PCR detected 29/39 and 25/39 positive samples of previously identifiedC. parvumandC. hominisDNA, respectively. The SAM-1 LAMP detected 27/39. On detection ofCryptosporidiumDNA in 67 clinical samples, the stem LFD LAMP detected 16 samples and SAM-2 LAMP 14 and nested PCR identified 11. Preheating the templates increased detection by stem LFD LAMP to 19 samples. Time to results from master mix preparation step took ~80 minutes. The test was specific, and no cross-amplification was recorded with nontarget DNA.Conclusion.The developed stem LFD LAMP test is an appropriate method for the detection ofC. hominis, C. parvum,andC. meleagridisDNA in human stool samples. It can be used in algorithm with other diagnostic tests and may offer promise as an effective diagnostic tool in the control of cryptosporidiosis.

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