Thèses sur le sujet « Aptameri »
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Wang, Tianjiao. « Function and dynamics of aptamers a case study on the malachite green aptamer / ». [Ames, Iowa : Iowa State University], 2008.
Trouver le texte intégralSavonnet, Maud. « Développement d'une méthode de détection innovante appliquée au diagnostic terrain des pathologies cardiaques ». Thesis, Université Grenoble Alpes, 2020. http://www.theses.fr/2020GRALY061.
Texte intégralToday, early diagnosis of cardiac pathologies is a major issue in healthcare world. Indeed, the speed of myocardial infarction diagnosis has an impact not only on the patient's health, but also on the management of emergency hospital services. The use of diagnostic devices at the patient’s bedside is a relevant solution to overcome effectively such a challenge. Consequently, the number of Point-Of-Care systems dedicated to the diagnosis of cardiac pathologies is growing. However, these devices have some disadvantages that need to be overcome.This thesis work has been conducted in this context. Research and development of an innovative method for the detection of cardiac biomarkers has been carried out. The objective of this method is the detection of any type of analyte in a complex medium with a good sensitivity allowed by the biomolecular amplification used. This generic method is based on the LAMP amplification of an oligonucleotide probe. It uses aptamer probes, specific to the target to be detected, which have been validated by surface plasmon resonance imaging. This method has been implemented in a relevant manner on different models and applied to the detection of a cardiac biomarker of interest, troponin I. The integration of this method in a portable microfluidic device was finally addressed for future use in field diagnostics
Dibenedetto, Silvia. « Direct activation of endogenous Calcineurin A : biological impact of selective peptide aptamers ». Phd thesis, Ecole normale supérieure de lyon - ENS LYON, 2011. http://tel.archives-ouvertes.fr/tel-00757018.
Texte intégralKimura, Mari, et 木村摩利. « Towards intracellular aptamers : delivery of anti-SCV helicase aptamers and development of aptamers againstSATB1 ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48079893.
Texte intégralpublished_or_final_version
Biochemistry
Master
Master of Philosophy
Brothier, Fabien. « Développement d'outils bioanalytiques miniaturisés : greffage de biomolécules sur monolithes en capillaire couplés à la nanochromatographie pour l'analyse d'échantillons complexes ». Thesis, Paris 6, 2014. http://www.theses.fr/2014PA066299/document.
Texte intégralThe analysis of ultra-traces from complex matrices (environmental, foodstuff or biological) often requires a step of purification and preconcentration before their analysis by chromatographic separation methods. Therefore, extraction sorbents based on a molecular recognition mechanism can be developed and used for the selective extraction of target molecules thus rendering their quantitative analysis in complex samples more reliable and sensitive. These extraction sorbents may result, among others, from the immobilization of biomolecules such as antibodies and aptamers (i.e. oligonucleotides whose sequence is specific for a target molecule). This selective sample pretreatment step is particularly necessary when developing miniaturized devices such as separative microsystems on chip because of the decrease of the resolution that results from the use of a shorter length separation channel. In this context, the aim of our study was to develop miniaturized bioanalytical devices for the analysis of small molecules or proteins in complex samples. For the development of these devices, in-situ synthesis of a porous hybrid organic-inorganic monolith in capillaries (100 µm i.d.) by sol-gel approach was firstly optimized and characterized in terms of repeatability. Secondly, two model toxins of low molecular weight were chosen: microcystin-LR (MC-LR) and ochratoxin A (OTA). Monoclonal antibodies and aptamers specific to one and the other target molecules were then grafted on the monolithic capillaries. The resulting miniaturized immunosorbent (mIS) and oligosorbent (mOS) were then coupled on-line to nanoLC. Specific retention of MC-LR and OTA on the mIS and the mOS, respectively, was demonstrated in pure water. Synthesis repeatability and capacity of the miniaturized sorbents were evaluated. Finally, these miniaturized tools were applied to the selective extraction of MC-LR or OTA from complex samples, i.e. blue-green algae extracts, environmental waters or beer. In a third part, immobilized enzyme reactors (IMERs) were prepared by grafting two proteolytic enzymes (pepsin and trypsin) on monoliths in order to transpose the developed selective tools to the analysis of proteins. These IMERs were then coupled on-line to nanoLC-MS² for the analysis of a model protein, cytochrome C. Digestion yields on IMERs presented a good repeatability. However, digestion efficiency on the pepsin-based IMERs remains so far insufficient and grafting or digestion procedure needs to be readjusted
Aschl, Timothy. « Biochips based on silicon for detecting the interaction between aptamers and pathogens ». Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLX103/document.
Texte intégralRapid and sensitive detection of pathogenic targets play a crucial role in biosecurity. Biochips are ideal for this, as they allow easy and multiplex detection of targets. A crucial limitation in biochips is that they often suffer from low reliability and sensitivity. The goal of this thesis is to develop a stable and reproducible architecture for biochips based on an amorphous silicon carbon alloy (a-SiC:H) deposited on an aluminium back-reflector for reliable and sensitive detection of pathogens. On these biochips we introduced the interaction of the food and feed toxin ochratoxin A (OTA) with its 36mer aptamer AntiOTA as a model system. Aptamers (single strands of DNA) are ideal as probes for biochips as they display high specificity and affinity towards a wide range of targets (i.e. proteins, bacteria…). The well-controlled multi-step fabrication process consists of the reliable photochemical grafting of acid-terminated organic monolayers on silicon surfaces by robust Si C bonds, which in turn were functionalized with aptamers by stable peptide coupling. Carrying out this process on crystalline silicon allowed monitoring and quantification of every step by infrared spectroscopy (IR-ATR). The interaction OTA – AntiOTA was shown for the first time on surfaces by IR, and an IR in situ calibration allowed the quantification of OTA which was bound by the aptamers on the surface. The specificity of AntiOTA towards OTA was demonstrated by using a chemically similar molecule (warfarin), for which AntiOTA shows no affinity. The well-controlled protocols were transferred to the a-SiC:H biochip. The immobilized aptamers were hybridized with complementary and fluorescent-labeled DNA-strands. In presence of OTA, dehybridization of the complementary strands is expected, resulting in a decrease of fluorescent signal. Different lengths of complementary strands were compared, exhibiting up to 13% signal decrease due to OTA
Daniel, Camille. « Biopuce à aptamères anti-thrombine : exploration d'une technique alternative de détection ». Phd thesis, Université de Grenoble, 2013. http://tel.archives-ouvertes.fr/tel-00954086.
Texte intégralKittichan, Kanokphandharangkul. « Aptamer biosensors ». Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/39048.
Texte intégralBini, Alessandra. « Aptamers for biosensors ». Thesis, Cranfield University, 2008. http://dspace.lib.cranfield.ac.uk/handle/1826/4004.
Texte intégralDalton, Colette. « Aptamers as biosensors ». Thesis, University of Strathclyde, 2010. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=15484.
Texte intégralLe, Thao Thi. « Aptamers for proteomics ». Thesis, Imperial College London, 2008. http://hdl.handle.net/10044/1/1385.
Texte intégralMejri, Nawel. « Development of biosensors based on DNA aptamers for direct mycotoxins detection ». Thesis, Perpignan, 2016. http://www.theses.fr/2016PERP0010.
Texte intégralThis aim of this work is to develop ultrasensitive electrochemical biosensors with high affinity toward ochratoxine (OTA) and aflatoxine M1 (AFM1). In order to obtain the best analytical performances, we associated nano-materials in the transducer construction: conducting polypyrrole polymer and poly(amido-amine) dendrimères. Thanks to this association, we benefited from the conducting material’s electrical properties, and the large active detection surface dendrimers. For the bimolecular sensing part, we used specific DNA aptamers which allowed us to quantify mycotoxines at nM concentrations. In addition, the different aptamer based biosensors present a very large dynamic ranges. We also demonstrated through the use of different sizes of dendrimers, that the sensitivity depend not only in the affinity between bioreceptors and their target molecules, but also in the physico-chemical properties of the biosensor
Milovskij, Aleksandr Sergeevič. « Oligosaccharid-gerichtete RNA-Aptamere ». [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=981439365.
Texte intégralProske, Daniela. « Neuropeptid Y- und Prionprotein- spezifische Aptamere ». Diss., lmu, 2001. http://nbn-resolving.de/urn:nbn:de:bvb:19-3017.
Texte intégralNick, Thomas [Verfasser]. « Stability of split-aptamers / Thomas Nick ». Mainz : Universitätsbibliothek Mainz, 2017. http://d-nb.info/1122865783/34.
Texte intégralTan, Kei Xian. « Aptameric Formulation for Enhanced Biopharmaceutical Delivery ». Thesis, Curtin University, 2018. http://hdl.handle.net/20.500.11937/68408.
Texte intégralPohl, Andrea. « Beiträge zur chemisch-biologischen Oberflächenmodifikation von Nanodiamanten aus der Detonationssynthese ». Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-232137.
Texte intégralThe present study deals with the surface modification of nanodiamonds (ND) from detonation synthesis and the subsequent conjugation of both single and double stranded DNA to previously introduced functional groups. As starting materials two kinds of nanodiamond powders with unknown surface configuration were used. Both types of ND were characterized by electron-microscopic methods. Furthermore, commercially modified ND with defined surface configuration (amino and hydroxyl groups) were applied. Potential applications of ND require a mono-functional surface, that can be realized e. g. via oxidation or reduction of the primary functional groups introduced during the production process. The thereby generated secondary functions permit the covalent or non-covalent linking of further substances onto the surfaces of ND particles. Conjugation of DNA, as described here, onto the carboxyl-, hydroxyl- or aminomodified particle surfaces was accomplished by generating of amino, phosphodiester and isourea bonds. The success of conjugations has been examined by infrared spectroscopy and fluorescence microscopy. The fluorescence of conjugates based on fluorescent dyes bound to the DNA molecules. Furthermore, the fabrication of a colloidal ND suspension is described, of which the particle sizes and the Zeta potential have been determined. Colloidal suspensions facilitate various biological and medical applications of ND on the basis of low particle sizes. The presented results enlarge the state of knowledge about the conjugation of DNA on ND from detonation synthesis. The applied methodology may also be transferred to other substances like proteins or chemotherapeutics. In this way, functionalized particles have a big potential for further application in biomedicine and nanotechnology
Reinemann, Christine. « Aptamere als neue molekulare Erkennungselemente in Biosensoren / ». Leipzig : Helmholtz-Zentrum für Umweltforschung, 2007. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=016271117&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Texte intégralElsaadani, Moez. « Détection des Ochratoxines A dans la production alimentaire par l’utilisation d’aptacapteur capacitif ». Thesis, Montpellier, 2019. http://www.theses.fr/2019MONTG077.
Texte intégralMycotoxin contamination is a threat to the health and life of Humans and animals. One of the most common mycotoxin contaminating feed and foodstuffs is Ochratoxin A (OTA). OTA has a chronic toxic effect and has proved to be mutagenic, nephrotoxic, teratogenic, immunosuppressive, and carcinogenic molecule. Aptamer with their specific affinity for OTA was used in this paper to create an analytical technique. Several methods have been reported for the determination of OTA in foods. However, most of these methods could not be applied to a complex food as green coffee because the interfering native fluorescent molecules made the quantification very difficult. In this work, we mixed two separations based techniques to identify and quantify OTA in green coffee. Aptamer assisted ultrafiltration as separation technique based on the size of molecules was applied to separate the free OTA; the quantification of OTA was established by a high-performance liquid chromatography (HPLC-FD) with LOD of 0.05 ng/mL for OTA. Artificially contaminated green coffee displayed a good range of OTA recoveries up to 97.7%. The selected aptamer was used as a biorecognition element to functionalize a capacitive sensor for OTA detection. A capacitive aptasensor was developed for quantification of OTA based on modified anodized aluminum oxide with a LOD of 10-4 ng/mL. This method can be applied to the quantitative determination of OTA in green coffee at levels below the maximum levels proposed by the European Commission for green coffee (0.25 ng/mL). It also confirm that aptamers can be used as biorecognition element in diagnostic assays with commercial application for mycotoxin analysis. To our knowledge, it is the first label-free and electric capacitive aptasensor used to detect the OTA
Weinert, Ulrike. « Erzeugung funktionaler Schichten auf Basis von bakteriellen Hüllproteinen ». Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-119909.
Texte intégralUrmann, Katharina [Verfasser]. « Aptamer-based optical biosensors / Katharina Urmann ». Hannover : Technische Informationsbibliothek (TIB), 2018. http://d-nb.info/1166271978/34.
Texte intégralZhang, Yangyang. « A functionalised aptamer electrochemical biosensor platform ». Thesis, Imperial College London, 2011. http://hdl.handle.net/10044/1/6438.
Texte intégralChaou, Thinhinane. « Sélection d’aptamères anti-adénine ADN modifiés en présence de solvant organique. Application au développement de biocapteurs ». Thesis, Bordeaux, 2015. http://www.theses.fr/2015BORD0385/document.
Texte intégralDevelopment of in situ detection tools (IDT) is required for specific real time monitoringof chemical species. Efficient IDT is not only related to its affinity and ability todiscriminate between molecular variants, but moreover it must be adapted for operatingunder conditions imposed by the context of application. Among others, hightemperature, pH variation and presence of organic solvents may be mentioned. The aimof our project is to develop an aptamer operating in the presence of organic solvents. Forthis purpose, we opted for selection in presence of methanol. Limitations of this strategyare adaptability of SELEX technology and chemical diversity of nucleic acids. For thispurpose, we used library incorporating (5-(octa1,7-diynyl)-2’-deoxyuridine) dDOTPinstead of conventional thymine nucleotide. Our experimental strategy led to theselection of an aptamer that specifically recognises adenine in the presence of 25 % ofmethanol. dDOTP nucleotides are essential for adenine recognition; the selectedaptamer shares a purine rich motif with a previously described adenosine aptamer, butdisplays a specific structure motif, essential for operating in the presence of methanol.Furthermore, the ability of truncated variants of the selected aptamer to form arecognition module of biosensor was assessed in this study
Frigotto, Laura. « Studies on novel RNA ligands for CD4 ». Thesis, University of Oxford, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249252.
Texte intégralMuharemagic, Darija. « Aptamers as Enhancers of Oncolytic Virus Therapy ». Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32170.
Texte intégralJones, Louisa Alice School of Biotechnology And Biomolecular Sciences UNSW. « Aptamers to the hepatitis C virus polymerase ». Awarded by:University of New South Wales. School of Biotechnology And Biomolecular Sciences, 2005. http://handle.unsw.edu.au/1959.4/32734.
Texte intégralAl-Youssef, Nadia. « The Selection of Aptamers to CD20 and Their Application as Inhibitors of Complement Dependent Cytotoxicity ». Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/33182.
Texte intégralDing, Shu Gu Li-Qun. « Aptamer encoded nanopores as single molecule sensors ». Diss., Columbia, Mo. : University of Missouri--Columbia, 2008. http://hdl.handle.net/10355/5767.
Texte intégralNguyen, Thi-Huong [Verfasser]. « Rupture forces of split aptamers / Thi-Huong Nguyen ». Mainz : Universitätsbibliothek Mainz, 2013. http://d-nb.info/1034486209/34.
Texte intégralSimmons, Suzanne Clare. « Development of Aptamers as Diagnostic and Therapeutic Agents ». Thesis, Open University, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.524784.
Texte intégralAptekar, Shraddha Ashok. « Selective targeting to glioma with nucleic acid aptamers ». Thesis, University of Central Lancashire, 2015. http://clok.uclan.ac.uk/11801/.
Texte intégralLeonard, Marissa. « Overcoming Breast Cancer Metastasis with Novel RNA Aptamers ». University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1572879601351414.
Texte intégralMarquardt, Janice Dionne. « Force interaction characterization between thrombin and DNA aptamers ». [Ames, Iowa : Iowa State University], 2008.
Trouver le texte intégralDassie, Justin Patrick. « Selective targeting of cancer cells with RNA aptamers ». Diss., University of Iowa, 2012. https://ir.uiowa.edu/etd/1310.
Texte intégralBrackett, David Michael. « Ligand binding and catalysis in an RNA aptamer / ». For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.
Texte intégralFordham, Daniel George. « Development of a biosensor using aptamer/antigen interactions ». Thesis, University of Portsmouth, 2011. https://researchportal.port.ac.uk/portal/en/theses/development-of-a-biosensor-using-aptamerantigen-interactions(66fc5f4e-0248-41d1-8124-d68b6b040a64).html.
Texte intégralLaurenson, Sophie. « The development and application of peptide aptamer microarrays ». Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613244.
Texte intégralWoodman, Robbie. « The development and application of peptide aptamer technology ». Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614795.
Texte intégralBartley, Amanda Nicole. « Aptamer-Based Assay For Detection Of Ochratoxin A ». FIU Digital Commons, 2018. https://digitalcommons.fiu.edu/etd/3894.
Texte intégralMeini, Nadir. « Conception et réalisation de biocapteurs impédimétriques ». Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10074/document.
Texte intégralThe objective of the research concerns the design and realization of biosensors based impedimetric measures, for which there is strong demand in various societal benefit areas, particularly the environment, food security and biomedical.Biosensors are rapid, selective and inexpensive devices that combine a biological recognition element, the so-called bioreceptor (e.g. enzymes, antibodies, DNA or microorganisms) to a physical transducer (e.g. electrochemical, optical, thermal or piezoelectrical). They can be used to detect one specific analyte or one family of analytes for a wide range of applications (e.g. environment, food, health). In the first part of this work, an aptasensor was developed for thrombin detection. Two different aptamers targeting thrombin were directly immobilized on the gold electrode. The aptasensor exhibits high sensitivity, specificity and stability in the detection of thrombin. In the second part, using electrochemical impedance spectroscopy (EIS), we have, monitored label-free protein immobilization on a gold surface, through a strategy of electroaddressing, compatible with the production of microarrays for multi-detection. This functionalization is achieved via the alkyne/azide cycloaddition, better known as the "click" reaction.Finally, a biosensor using phage proteins was developed for detecting E. coli
Haarberg, Hans Eirik. « Theophylline IMAGEtags (intracellular multi aptamer genetic tags) the development and evaluation of an RNA reporter system based on the theophylline aptamer / ». [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1464205.
Texte intégralBayrac, Abdullah Tahir. « In Vitro Selection Of Dna Aptamers To Glioblastoma Multiforme ». Phd thesis, METU, 2011. http://etd.lib.metu.edu.tr/upload/12613769/index.pdf.
Texte intégralLennarz, Sabine [Verfasser]. « RNA aptamers as selective protein kinase inhibitors / Sabine Lennarz ». Bonn : Universitäts- und Landesbibliothek Bonn, 2015. http://d-nb.info/1077289588/34.
Texte intégralMeyer, Michael [Verfasser]. « Applications of aptamers in flow cytometry assays / Michael Meyer ». Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2014. http://d-nb.info/1059512718/34.
Texte intégralZhang, Naru, et 张娜茹. « Study on influenza virus-like particles and ssDNA aptamers ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/200167.
Texte intégralpublished_or_final_version
Microbiology
Doctoral
Doctor of Philosophy
Ellingham, Mark David. « Selection and characterisation of RNA aptamers to FMDV 3Dpol ». Thesis, University of Leeds, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.436396.
Texte intégralDing, Yindi. « Peptide aptamers : tools for the analysis of RAS signaling ». Lyon, École normale supérieure (sciences), 2009. http://www.theses.fr/2009ENSL0535.
Texte intégralThis thesis describes the use of peptide aptamers (PA) for the study of Ras signaling in model cell lines. It further describes the initial analysis of biological effects of small molecules identified for their capacity to displace a biologicallyactive PA from H-RasV12. Finally, it offers characterization of DRGn precursors that we hope to be able to use for future studies. To analyze Ras interactions with other proteins and perturbation of signaling pathways, PAs directed towards wt Ras and oncogenic Ras had been selected. PA 105R elicited an interaction phenotype in yeast two–hybrid (Y2H) with H-, K-, and N-Ras. PA HR3C gave an interaction phenotype with H-Ras wt and HrasV12. In PC12 cells, it blocked both wt and oncogenic H-Ras signaling, whereas 105R had little effect on NGF-induced signaling, but inhibited that stimulated by oncogenic Ras. In contrast, in AsPC-1, endogenous oncogenic K-RasD12 is inhibited by 105R, while HR3C is much less effective. Targeted mutation of amino acids located on the surface of H-RasV12, followed by Y2H interaction mating assays, offered the possibility to map the binding sites of the biologically-active Pas. We evaluated biological activity of a number of small molecules selected for their capacity to displace one of the Ras-specific PAs from the target. These studies resulted in the identification of one molecule that may be biologically pertinent. Further studies will be required to develop possible pre-clinical candidates based on these molecules. Finally, we were to implement the PA technology in primary cells derived from rat DRG, and we developed a DRG precursor line that we have maintained in culture for over two years. It is hoped that we use thes precursors for future studies in the PNS
Gibert, Benjamin. « La protéine de stress Hsp27 / HspB1, une cible de choix en thérapie anti-cancéreuse ». Phd thesis, Université Claude Bernard - Lyon I, 2010. http://tel.archives-ouvertes.fr/tel-00733751.
Texte intégralCatlin, Diane M. « DNA Aptamer Confirmation and Utilization for the Cyanotoxin, Cylindrospermopsin ». FIU Digital Commons, 2016. http://digitalcommons.fiu.edu/etd/2552.
Texte intégralMehanovic, Samir. « Development of an RNA aptamer to PD173955N through SELEX ». [Ames, Iowa : Iowa State University], 2008.
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