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1

Lee, SangWook, Byung Woo Kim, Hye-Su Shin, Anna Go, Min-Ho Lee, Dong-Ki Lee, Soyoun Kim et Ok Chan Jeong. « Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis ». Micromachines 10, no 11 (11 novembre 2019) : 770. http://dx.doi.org/10.3390/mi10110770.

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Here, we report a simple and effective method for capturing and displacement of gram-negative bacteria using aptamer-modified microbeads and acoustophoresis. As acoustophoresis allows for simultaneous washing and size-dependent separation in continuous flow mode, we efficiently obtained gram-negative bacteria that showed high affinity without any additional washing steps. The proposed device has a simple and efficient channel design, utilizing a long, square-shaped microchannel that shows excellent separation performance in terms of the purity, recovery, and concentration factor. Microbeads (10 µm) coated with the GN6 aptamer can specifically bind gram-negative bacteria. After incubation of bacteria culture sample with aptamer affinity bead, gram-negative bacteria-bound microbeads, and other unbound/contaminants can be separated by size with high purity and recovery. The device demonstrated excellent separation performance, with high recovery (up to 98%), high purity (up to 99%), and a high-volume rate (500 µL/min). The acoustophoretic separation performances were conducted using 5 Gram-negative bacteria and 5 Gram-positive bacteria. Thanks to GN6 aptamer’s binding affinity, aptamer affinity bead also showed binding affinity to multiple strains of gram-negative bacteria, but not to gram-positive bacteria. GN6 coated bead can capture Gram-negative bacteria but not Gram-positive bacteria. This study may present a different perspective in the field of early diagnosis in bacterial infectious diseases. In addition to detecting living bacteria or bacteria-derived biomarkers, this protocol can be extended to monitoring the contamination of water resources and may aid quick responses to bioterrorism and pathogenic bacterial infections.
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Cobb, Corie Lynn, Matthew R. Begley, Emilee Nicole Armstrong et Keith Edward Johnson. « (Invited) Manufacturing 3D Electrode Architectures Via Acoustophoresis ». ECS Meeting Abstracts MA2022-02, no 6 (9 octobre 2022) : 608. http://dx.doi.org/10.1149/ma2022-026608mtgabs.

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Achieving high-energy and high-power density Lithium-ion batteries (LIBs) with fast charge behavior is critical for the future of electric vehicle applications. Conventional LIBs have planar anode and cathode electrode stacks that can be optimized for energy or power, but not both simultaneously due to fundamental ion transport limitations with increasing electrode thickness. Three-dimensional (3D) electrode architectures1,2 can remove these performance trade-offs through engineered ion-transport in thick electrodes. However, scalable manufacturing methods for patterning these architectures over large areas at meaningful time scales is still limited. As a path to solving this challenge, we leverage both modeling and experiments to investigate the feasibility of deploying acoustophoresis to assemble and pattern 3D battery electrodes. Acoustophoresis employs acoustic standing waves to focus particles and enables near micron-scale control over particle placement in a fluid medium at time scales < 1 second. Prior research has shown the potential for rapid assembly of particles with this approach,3,4 making acoustic-based processing a promising methodology for manufacturing 3D electrodes over large areas. In this talk, we expand a previously developed model4 that solves differential equations of acoustic forces to track particle trajectories and define how the acoustic forces are influenced by slurry viscosity, particle loading, and particle morphology. Our initial experiments with different material systems, including LiNi0.6Mn0.2Co0.2O2 (NMC-622), help validate our model and process conditions to as a path towards acoustophoretic fabrication of 3D electrode architectures. References C. L. Cobb and S. E. Solberg, J. Electrochem. Soc., 164, A1339–A1341 (2017). C. L. Cobb and M. Blanco, Journal of Power Sources, 249, 357–366 (2014). D. S. Melchert et al., Materials & Design, 109512 (2021). R. R. Collino et al., Materials Research Letters, 6, 191–198 (2018). Acknowledgements This material is based upon work supported by the U.S. Department of Energy’s Office of Energy Efficiency and Renewable Energy (EERE) under the Advanced Manufacturing Office (AMO) Award Number DE-EE0009112. The views expressed herein do not necessarily represent the views of the U.S. Department of Energy or the United States Government.
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Tandar, Clara E., Ryan Dubay, Eric Darling et Jason Fiering. « Cell-like microparticles with tunable acoustic properties for calibrating devices ». Journal of the Acoustical Society of America 152, no 4 (octobre 2022) : A36. http://dx.doi.org/10.1121/10.0015454.

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Mechanophenotype of biological cells has demonstrated correlation with biomolecular states and cell function. Hence, new methods to measure mechanophenotype at high throughput are of growing interest. Acoustophoretic microdevices can characterize cell mechanical features; however, calibration particles with physiologically relevant properties are needed to quantify and optimize device performance. Currently, conventional polymer microspheres are rigid and do not replicate cell deformation and compressibility. To address this, we developed monodisperse, tunable, cell-like microparticles (MPs) from polyacrylamide hydrogel, fabricated with a microfluidic droplet generator. Size and compressibility are adjusted by fabrication parameters, and density is adjusted by incorporation of nanoparticles (NPs). Here, we present for the first time microparticles of reduced density and acoustic contrast (lower than unloaded MPs) achieved by loading MPs with nanoparticles of low molecular weight alkanes. We produced the NPs by sonication and photopolymerization before addition to the MP precursor. NP-loaded MPs were less dense than unloaded MPs at 1005.9 and 1013.6kg/m3, respectively, and they exhibited negative acoustic contrast by acoustophoresis in aqueous medium while that of unloaded MPs was positive. These new particles extend the tunable range of acoustic contrast, mimicking and exceeding that of most biological cells and could also aid cell separation when conjugated to cells.
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Heyman, Joseph S. « Acoustophoresis separation method ». Journal of the Acoustical Society of America 94, no 2 (août 1993) : 1176–77. http://dx.doi.org/10.1121/1.406934.

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Leibacher, Ivo, Alexander Garbin, Philipp Hahn et Jürg Dual. « Acoustophoresis of Disks ». Physics Procedia 70 (2015) : 21–24. http://dx.doi.org/10.1016/j.phpro.2015.08.017.

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Hsu, Jin-Chen, Chih-Hsun Hsu et Yeo-Wei Huang. « Acoustophoretic Control of Microparticle Transport Using Dual-Wavelength Surface Acoustic Wave Devices ». Micromachines 10, no 1 (13 janvier 2019) : 52. http://dx.doi.org/10.3390/mi10010052.

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We present a numerical and experimental study of acoustophoretic manipulation in a microfluidic channel using dual-wavelength standing surface acoustic waves (SSAWs) to transport microparticles into different outlets. The SSAW fields were excited by interdigital transducers (IDTs) composed of two different pitches connected in parallel and series on a lithium niobate substrate such that it yielded spatially superimposed and separated dual-wavelength SSAWs, respectively. SSAWs of a singltablee target wavelength can be efficiently excited by giving an RF voltage of frequency determined by the ratio of the velocity of the SAW to the target IDT pitch (i.e., f = cSAW/p). However, the two-pitch IDTs with similar pitches excite, less efficiently, non-target SSAWs with the wavelength associated with the non-target pitch in addition to target SSAWs by giving the target single-frequency RF voltage. As a result, dual-wavelength SSAWs can be formed. Simulated results revealed variations of acoustic pressure fields induced by the dual-wavelength SSAWs and corresponding influences on the particle motion. The acoustic radiation force in the acoustic pressure field was calculated to pinpoint zero-force positions and simulate particle motion trajectories. Then, dual-wavelength SSAW acoustofluidic devices were fabricated in accordance with the simulation results to experimentally demonstrate switching of SSAW fields as a means of transporting particles. The effects of non-target SSAWs on pre-actuating particles were predicted and observed. The study provides the design considerations needed for the fabrication of acoustofluidic devices with IDT-excited multi-wavelength SSAWs for acoustophoresis of microparticles.
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Foresti, Daniele, Katharina T. Kroll, Robert Amissah, Francesco Sillani, Kimberly A. Homan, Dimos Poulikakos et Jennifer A. Lewis. « Acoustophoretic printing ». Science Advances 4, no 8 (août 2018) : eaat1659. http://dx.doi.org/10.1126/sciadv.aat1659.

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Urbansky, Anke, Andreas Lenshof, Josefina Dykes, Thomas Laurell et Stefan Scheding. « ­Separation of Lymphocyte Populations from Peripheral Blood Progenitor Cell Products Using Affinity Bead Acoustophoresis ». Blood 124, no 21 (6 décembre 2014) : 315. http://dx.doi.org/10.1182/blood.v124.21.315.315.

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Abstract Introduction: Processing of peripheral blood progenitor cells (PBPC) for clinical transplantation or research applications aims to effectively isolate or deplete specific cell populations. We have previously reported the use of a novel ultrasound-based sorting technology, called acoustophoresis, for sorting of platelets (Dykes et al., PloS one 2011) and CD4+ cells from PBPC products (Lenshof et al., Cytometry Part A 2014). Here, we investigated the performance of microfluidic acoustophoresis for the separation of CD8+ lymphocytes from PBPC, and present a method for affinity-bead-mediated acoustic separation of cells which otherwise cannot be discriminated acoustically. In an acoustic standing wave field radiation forces induce movement of particles depending on particle and medium properties, such as for example particle size, density and compressibility. Targeting of cells by affinity specific beads generates cell-bead complexes that exhibit distinct acoustic properties relative to non-targeted cells and, thus, become possible to isolate. Method: PBPC samples (n=16) were obtained from patients and healthy donors. Following density gradient centrifugation, mononuclear cells were labelled with anti-CD8 microbeads (Dynal) and sorted either on an acoustophoresis-microchip (Figure 1) or standard magnetic cell sorting technique for comparison. PBPC samples, target and waste fractions were analysed for purity, separation efficiency, recovery, T-cell function and progenitor cell content. Results: PBPC products contained a mean of 11.6 ± 7.1% CD8+ cells before sorting. Purities obtained with acoustic sorting of CD8+ lymphocytes were 93.3 ± 6.8% compared to 94.4 ± 8.6% for magnetic sorting (n=16). Viabilities of sorted cells were 97.0 ± 3.9% (acoustic) and 97.5 ± 3.5% (magnetic). Mean separation efficiency recovery of acoustic sorted CD8+ cells was 57 ± 19% of the total CD8+ cells compared to a median recovery of magnetic sorted CD8+ cells of 43 ± 17%. Leukocyte subpopulation analysis performed after CD8 selection showed a relative increase of CD4 cells in the non-target fractions due to the removal of CD8 cells. Functional testing of sorted CD8+ lymphocytes showed unimpaired mitogen mediated proliferation capacity after 2-day, 4-day and 6-day stimulation with CD3/CD28. Furthermore, hematopoietic progenitor cell assays revealed a preserved colony forming ability of the post-sorted non-target cells Conclusion: Acoustophoresis is a promising technology to efficiently sort bead-labelled lymphocyte populations from PBPC samples with high purity and recovery without impairing lymphocyte function. Affinity-bead acoustophoresis is, thus, an interesting technology for stem cell processing in PBPC. Figure 1 Picture of the acoustophoresis platform. The cell suspension with bead-labeled CD8+ cells enters through the side inlets (a) while the wash buffer (Histopaque-1077) is injected through the center inlet (b). Radiation forces in the acoustic standing wave field move the cell-bead complex faster to the center compared to non-target cells and can be separated in the center outlet of the channel (c). Non-target cells exit through the side outlets (d). The total length of the acoustophoresis microchip is 35mm. Figure 1. Picture of the acoustophoresis platform. The cell suspension with bead-labeled CD8+ cells enters through the side inlets (a) while the wash buffer (Histopaque-1077) is injected through the center inlet (b). Radiation forces in the acoustic standing wave field move the cell-bead complex faster to the center compared to non-target cells and can be separated in the center outlet of the channel (c). Non-target cells exit through the side outlets (d). The total length of the acoustophoresis microchip is 35mm. Disclosures Laurell: Acousort AB: shareholder Other. Scheding:Acousort AB: Co-founder and board member Other.
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Brooks, Todd L., et Robert E. Apfel. « Novel configurations for acoustophoresis ». Journal of the Acoustical Society of America 102, no 5 (novembre 1997) : 3184. http://dx.doi.org/10.1121/1.420853.

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Baasch, Thierry, Ivo Leibacher et Jürg Dual. « Multibody dynamics in acoustophoresis ». Journal of the Acoustical Society of America 141, no 3 (mars 2017) : 1664–74. http://dx.doi.org/10.1121/1.4977030.

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Koklu, Mehti, Ahmet Can Sabuncu et Ali Beskok. « Acoustophoresis in shallow microchannels ». Journal of Colloid and Interface Science 351, no 2 (novembre 2010) : 407–14. http://dx.doi.org/10.1016/j.jcis.2010.08.029.

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Leibacher, Ivo, Peter Reichert et Jürg Dual. « Microfluidic droplet handling by bulk acoustic wave (BAW) acoustophoresis ». Lab on a Chip 15, no 13 (2015) : 2896–905. http://dx.doi.org/10.1039/c5lc00083a.

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Mao, Zhangming, Yuliang Xie, Feng Guo, Liqiang Ren, Po-Hsun Huang, Yuchao Chen, Joseph Rufo, Francesco Costanzo et Tony Jun Huang. « Experimental and numerical studies on standing surface acoustic wave microfluidics ». Lab on a Chip 16, no 3 (2016) : 515–24. http://dx.doi.org/10.1039/c5lc00707k.

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Cacace, Teresa, Vittorio Bianco, Melania Paturzo, Pasquale Memmolo, Massimo Vassalli, Massimiliano Fraldi, Giuseppe Mensitieri et Pietro Ferraro. « Retrieving acoustic energy densities and local pressure amplitudes in microfluidics by holographic time-lapse imaging ». Lab on a Chip 18, no 13 (2018) : 1921–27. http://dx.doi.org/10.1039/c8lc00149a.

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Reichert, Peter, Dhananjay Deshmukh, Lukas Lebovitz et Jürg Dual. « Thin film piezoelectrics for bulk acoustic wave (BAW) acoustophoresis ». Lab on a Chip 18, no 23 (2018) : 3655–67. http://dx.doi.org/10.1039/c8lc00833g.

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Akella, Meghana, Soheila Shabaniverki et Jaime J. Juárez. « Acoustophoretic assembly of millimeter-scale Janus fibers ». RSC Advances 10, no 1 (2020) : 434–43. http://dx.doi.org/10.1039/c9ra09796a.

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Urbansky, Anke, Franziska Olm, Stefan Scheding, Thomas Laurell et Andreas Lenshof. « Label-free separation of leukocyte subpopulations using high throughput multiplex acoustophoresis ». Lab on a Chip 19, no 8 (2019) : 1406–16. http://dx.doi.org/10.1039/c9lc00181f.

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Dolatmoradi, Ata, et Bilal El-Zahab. « Thermally-assisted ultrasonic separation of giant vesicles ». Lab on a Chip 16, no 18 (2016) : 3449–53. http://dx.doi.org/10.1039/c6lc00765a.

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Vidal, S. Durand, J. P. Simonin, P. Turq et O. Bernard. « Acoustophoresis Revisited. 1. Electrolyte Solutions ». Journal of Physical Chemistry 99, no 17 (avril 1995) : 6733–38. http://dx.doi.org/10.1021/j100017a065.

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Md Ali, Mohd Anuar, Kostya (Ken) Ostrikov, Fararishah Abdul Khalid, Burhanuddin Y. Majlis et Aminuddin A. Kayani. « Active bioparticle manipulation in microfluidic systems ». RSC Advances 6, no 114 (2016) : 113066–94. http://dx.doi.org/10.1039/c6ra20080j.

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The motion of bioparticles in a microfluidic environment can be actively controlled using several tuneable mechanisms, including hydrodynamic, electrophoresis, dielectrophoresis, magnetophoresis, acoustophoresis, thermophoresis and optical forces.
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Iranmanesh, Ida, Harisha Ramachandraiah, Aman Russom et Martin Wiklund. « On-chip ultrasonic sample preparation for cell based assays ». RSC Advances 5, no 91 (2015) : 74304–11. http://dx.doi.org/10.1039/c5ra16865a.

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We demonstrate pre-alignment, size-based separation, isolation, trapping, up-concentration and fluorescence monitoring of cells in a sequence by the use of a multi-step, three-transducer acoustophoresis chip designed for cellular sample preparation.
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Hirayama, Ryuji, et Sriram Subramanian. « Magical multi-modal displays using acoustophoresis ». XRDS : Crossroads, The ACM Magazine for Students 29, no 1 (septembre 2022) : 54–58. http://dx.doi.org/10.1145/3558195.

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Le Magueresse, Romain, Tamara Krpic, Maxime Bilodeau, Robert Schiavi, Pierre Gelinas et Nicolas Quaegebeur. « Axisymmetric acoustophoresis for paper pulp concentration ». Ultrasonics Sonochemistry 80 (décembre 2021) : 105822. http://dx.doi.org/10.1016/j.ultsonch.2021.105822.

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Brooks, Todd L., et Robert E. Apfel. « Studies of particle separation using acoustophoresis ». Journal of the Acoustical Society of America 105, no 2 (février 1999) : 1017. http://dx.doi.org/10.1121/1.424872.

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Bruus, Henrik. « Acoustofluidics 10 : Scaling laws in acoustophoresis ». Lab on a Chip 12, no 9 (2012) : 1578. http://dx.doi.org/10.1039/c2lc21261g.

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Evander, Mikael, Andreas Lenshof, Thomas Laurell et Johan Nilsson. « Acoustophoresis in Wet-Etched Glass Chips ». Analytical Chemistry 80, no 13 (juillet 2008) : 5178–85. http://dx.doi.org/10.1021/ac800572n.

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Yu, Gan, Xiaolin Chen et Jie Xu. « Acoustophoresis in variously shaped liquid droplets ». Soft Matter 7, no 21 (2011) : 10063. http://dx.doi.org/10.1039/c1sm05871a.

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Augustsson, Per, Jonas Persson, Simon Ekström, Mats Ohlin et Thomas Laurell. « Decomplexing biofluids using microchip based acoustophoresis ». Lab Chip 9, no 6 (2009) : 810–18. http://dx.doi.org/10.1039/b811027a.

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Vijayakumar, V., J. M. Dabbi, J. L. Walker, A. Mertiri, R. J. Christianson et J. Fiering. « Rosette-induced separation of T cells by acoustophoresis ». Biomicrofluidics 16, no 5 (septembre 2022) : 054107. http://dx.doi.org/10.1063/5.0109017.

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Breakthrough cell therapies for the treatment of cancers require the separation of specific cells, such as T cells, from the patient's blood. Current cell therapy processes rely on magnetic separation, which adds clinical risk and requires elevated manufacturing controls due to the added foreign material that constitutes the magnetic beads. Acoustophoresis, a method that uses ultrasound for cell separation, has demonstrated label-free enrichment of T cells from blood, but residual other lymphocytes limit the ultimate purity of the output T cell product. Here, to increase the specificity of acoustophoresis, we use affinity reagents to conjugate red blood cells with undesired white blood cells, resulting in a cell–cell complex (rosette) of increased acoustic mobility. We achieve up to 99% purity of T cells from blood products, comparable to current standards of magnetic separation, yet without the addition of separation particles.
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Dong, Zhengya, David Fernandez Rivas et Simon Kuhn. « Acoustophoretic focusing effects on particle synthesis and clogging in microreactors ». Lab on a Chip 19, no 2 (2019) : 316–27. http://dx.doi.org/10.1039/c8lc00675j.

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Hahn, Philipp, Olivier Schwab et Jurg Dual. « Modeling and optimization of acoustofluidic micro-devices ». Lab Chip 14, no 20 (2014) : 3937–48. http://dx.doi.org/10.1039/c4lc00714j.

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Dow, P., K. Kotz, S. Gruszka, J. Holder et J. Fiering. « Acoustic separation in plastic microfluidics for rapid detection of bacteria in blood using engineered bacteriophage ». Lab on a Chip 18, no 6 (2018) : 923–32. http://dx.doi.org/10.1039/c7lc01180f.

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Antfolk, Maria, Christian Antfolk, Hans Lilja, Thomas Laurell et Per Augustsson. « A single inlet two-stage acoustophoresis chip enabling tumor cell enrichment from white blood cells ». Lab on a Chip 15, no 9 (2015) : 2102–9. http://dx.doi.org/10.1039/c5lc00078e.

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Ohlin, Mathias, Ida Iranmanesh, Athanasia E. Christakou et Martin Wiklund. « Temperature-controlled MPa-pressure ultrasonic cell manipulation in a microfluidic chip ». Lab on a Chip 15, no 16 (2015) : 3341–49. http://dx.doi.org/10.1039/c5lc00490j.

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We study the effect of 1 MPa-pressure ultrasonic-standing-wave trapping of cells during one hour in a fully temperature- and acoustic streaming-controlled microfluidic chip, and conclude that the viability of lung cancer cells are not affected by this high-pressure, long-term acoustophoresis treatment.
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Karthick, S., P. N. Pradeep, P. Kanchana et A. K. Sen. « Acoustic impedance-based size-independent isolation of circulating tumour cells from blood using acoustophoresis ». Lab on a Chip 18, no 24 (2018) : 3802–13. http://dx.doi.org/10.1039/c8lc00921j.

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Here, we report a label-free method based on acoustic impedance contrast for the isolation of CTCs from peripheral blood mononuclear cells (PBMCs) in a microchannel using acoustophoresis. Applying this method, we demonstrate the label-free isolation of HeLa and MDA-MB-231 cells from PBMCs.
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Flatt, R. J. « Acoustophoretic characterization of cement suspensions ». Materials and Structures 35, no 253 (27 septembre 2002) : 541–49. http://dx.doi.org/10.1617/13729.

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Bettiga, M., C. Grenvall et T. Laurell. « Acoustophoretic Separation of Yeast Cells ». Journal of Biotechnology 150 (novembre 2010) : 522. http://dx.doi.org/10.1016/j.jbiotec.2010.09.837.

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Flatt, R. J., et C. F. Ferraris. « Acoustophoretic characterization of cement suspensions ». Materials and Structures 35, no 9 (novembre 2002) : 541–49. http://dx.doi.org/10.1007/bf02483122.

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Lenshof, A., et T. Laurell. « Emerging Clinical Applications of Microchip-Based Acoustophoresis ». Journal of Laboratory Automation 16, no 6 (décembre 2011) : 443–49. http://dx.doi.org/10.1016/j.jala.2011.07.004.

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Hilgenfeldt, Sascha, Bhargav Rallabandi, Siddhansh Agarwal et David Raju. « Beyond acoustophoresis : Particle manipulation near oscillating interfaces ». Journal of the Acoustical Society of America 141, no 5 (mai 2017) : 3463. http://dx.doi.org/10.1121/1.4987189.

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Nama, Nitesh, Rune Barnkob, Zhangming Mao, Christian J. Kähler, Francesco Costanzo et Tony Jun Huang. « Numerical study of acoustophoretic motion of particles in a PDMS microchannel driven by surface acoustic waves ». Lab on a Chip 15, no 12 (2015) : 2700–2709. http://dx.doi.org/10.1039/c5lc00231a.

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Soh, Tom H., et Allen Yang. « Acoustophoretic cell sorting in microfluidic channels ». Journal of the Acoustical Society of America 132, no 3 (septembre 2012) : 1952. http://dx.doi.org/10.1121/1.4755183.

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Foresti, Daniele, et Dimos Poulikakos. « Acoustophoretic Waltz : A contactless exothermal reaction ». Physics of Fluids 26, no 9 (septembre 2014) : 091111. http://dx.doi.org/10.1063/1.4893545.

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Adams, Jonathan D., et H. Tom Soh. « Tunable acoustophoretic band-pass particle sorter ». Applied Physics Letters 97, no 6 (9 août 2010) : 064103. http://dx.doi.org/10.1063/1.3467259.

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Brooks, Todd L., et Robert E. Apfel. « Simulation of particle motions in an acoustophoresis device ». Journal of the Acoustical Society of America 109, no 5 (mai 2001) : 2346. http://dx.doi.org/10.1121/1.4744243.

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Fornell, Anna, Kevin Cushing, Johan Nilsson et Maria Tenje. « Binary particle separation in droplet microfluidics using acoustophoresis ». Applied Physics Letters 112, no 6 (5 février 2018) : 063701. http://dx.doi.org/10.1063/1.5020356.

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Dubay, R., C. Lissandrello, P. Swierk, N. Moore, D. Doty et J. Fiering. « Scalable high-throughput acoustophoresis in arrayed plastic microchannels ». Biomicrofluidics 13, no 3 (mai 2019) : 034105. http://dx.doi.org/10.1063/1.5096190.

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Lin, Sz-Chin Steven, Xiaole Mao et Tony Jun Huang. « Surface acoustic wave (SAW) acoustophoresis : now and beyond ». Lab on a Chip 12, no 16 (2012) : 2766. http://dx.doi.org/10.1039/c2lc90076a.

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Kothapalli, Satya V. V. N., Martin Wiklund, Birgitta Janerot-Sjoberg, Gaio Paradossi et Dmitry Grishenkov. « Investigation of polymer-shelled microbubble motions in acoustophoresis ». Ultrasonics 70 (août 2016) : 275–83. http://dx.doi.org/10.1016/j.ultras.2016.05.016.

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Brooks, Todd L., et Robert E. Apfel. « Design and characterization of a system for acoustophoresis ». Journal of the Acoustical Society of America 107, no 5 (mai 2000) : 2845. http://dx.doi.org/10.1121/1.429195.

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