Bibliographies thématiques / 3D motility

Littérature scientifique sur le sujet « 3D motility »

Auteur : Grafiati

Publié le 11 mars 2023

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Articles de revues sur le sujet "3D motility"

Bouchet, Benjamin P., et Anna Akhmanova. « Microtubules in 3D cell motility ». Journal of Cell Science 130, n^o 1 (1 janvier 2017) : 39–50. http://dx.doi.org/10.1242/jcs.189431.

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Bhattacharjee, Tapomoy, et Thomas E. Angelini. « 3D T cell motility in jammed microgels ». Journal of Physics D : Applied Physics 52, n^o 2 (2 novembre 2018) : 024006. http://dx.doi.org/10.1088/1361-6463/aae813.

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Nors, Jesper, Mette Winther Klinge, Thorbjørn Sommer, Søren Laurberg, Klaus Krogh et Jonas Amstrup Funder. « Assessment of postoperative gastrointestinal motility in colorectal surgery : a study with the Motilis 3D-transit system ». BMJ Innovations 7, n^o 1 (25 novembre 2020) : 53–60. http://dx.doi.org/10.1136/bmjinnov-2019-000396.

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PurposePostoperative recovery following colorectal surgery remains impaired by severe complications including postoperative ileus (POI). Human studies of POI have been limited by a lack of safe and easy-to-use objective methods. Motilis 3D-transit is a completely ambulatory, minimally invasive system whereby electromagnetic capsules are followed by external sensors during their passage of the gastrointestinal (GI) tract. The aim of this study was to evaluate the applicability of the 3D-transit system in a surgical setting.MethodWe included 12 patients as a substudy of the randomised double blind controlled Stimulation of the Autonomic Nervous System In Colorectal Surgery by perioperative nutrition (SANICS)-II trial undergoing elective segmental colonic resection with primary anastomosis at Aarhus University Hospital and Randers Regional Hospital, Denmark. To study region-specific motility, three electromagnetic capsules were administered. One was taken 3 hours before surgery, the next was taken 1 hour before surgery, while the third was placed distal to the anastomosis during surgery. Total and regional GI transit times as well as time until first propulsive colonic contraction were determined.ResultsAll patients tolerated the setup well with no adverse events related to the 3D-transit system. Large variations were found in total GI transit time (26.7–127.6 hours), gastric emptying (0.07–>106.9 hours), small intestinal (1.2–58.4 hours) and colorectal transit time (14.3–>118.1 hours). Time from end of surgery to first propulsive movement in the colon varied from 3.9 to 85 hours. No correlation was found between parameters of GI motility and tolerance of an oral diet or recovery of bowel function.ConclusionThe 3D-transit system allows safe assessment of GI motility in patients operated with segmental colonic resections and primary anastomosis for colorectal cancer. Postsurgical motility varies significantly between patients.

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Acres, Jacqueline, et Jay Nadeau. « 2D vs 3D tracking in bacterial motility analysis ». AIMS Biophysics 8, n^o 4 (2021) : 385–99. http://dx.doi.org/10.3934/biophy.2021030.

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<abstract> <p>Digital holographic microscopy provides the ability to observe throughout a large volume without refocusing. This capability enables simultaneous observations of large numbers of microorganisms swimming in an essentially unconstrained fashion. However, computational tools for tracking large 4D datasets remain lacking. In this paper, we examine the errors introduced by tracking bacterial motion as 2D projections vs. 3D volumes under different circumstances: bacteria free in liquid media and bacteria near a glass surface. We find that while XYZ speeds are generally equal to or larger than XY speeds, they are still within empirical uncertainties. Additionally, when studying dynamic surface behavior, the Z coordinate cannot be neglected.</p> </abstract>

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Siegel, Ashley L., Kevin Atchison, Kevin E. Fisher, George E. Davis et D. D. W. Cornelison. « 3D Timelapse Analysis of Muscle Satellite Cell Motility ». Stem Cells 27, n^o 10 (octobre 2009) : 2527–38. http://dx.doi.org/10.1002/stem.178.

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Belletti, Barbara, Ilenia Pellizzari, Stefania Berton, Linda Fabris, Katarina Wolf, Francesca Lovat, Monica Schiappacassi et al. « p27kip1 Controls Cell Morphology and Motility by Regulating Microtubule-Dependent Lipid Raft Recycling ». Molecular and Cellular Biology 30, n^o 9 (1 mars 2010) : 2229–40. http://dx.doi.org/10.1128/mcb.00723-09.

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ABSTRACT p27kip1 (p27) is an inhibitor of cyclin/cyclin-dependent kinase complexes, whose nuclear loss indicates a poor prognosis in various solid tumors. When located in the cytoplasm, p27 binds Op18/stathmin (stathmin), a microtubule (MT)-destabilizing protein, and restrains its activity. This leads to MT stabilization, which negatively affects cell migration. Here, we demonstrate that this p27 function also influences morphology and motility of cells immersed in three-dimensional (3D)matrices. Cells lacking p27 display a decrease in MT stability, a rounded shape when immersed in 3D environments, and a mesenchymal-amoeboid conversion in their motility mode. Upon cell contact to extracellular matrix, the decreased MT stability observed in p27 null cells results in accelerated lipid raft trafficking and increased RhoA activity. Importantly, cell morphology, motility, MT network composition, and distribution of p27 null cells were rescued by the concomitant genetic ablation of Stathmin, implicating that the balanced expression of p27 and stathmin represents a crucial determinant for cytoskeletal organization and cellular behavior in 3D contexts.

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Green, Jordan R., et Erin M. Wilson. « Spontaneous facial motility in infancy : A 3D kinematic analysis ». Developmental Psychobiology 48, n^o 1 (2005) : 16–28. http://dx.doi.org/10.1002/dev.20112.

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Lemos, Lauana Greicy Tonon, Gabriel Mello da Cunha Longo, Bruna dos Santos Mendonça, Marcela Cristina Robaina, Mariana Concentino Menezes Brum, Caíque de Assis Cirilo, Etel Rodrigues Pereira Gimba et al. « The LQB-223 Compound Modulates Antiapoptotic Proteins and Impairs Breast Cancer Cell Growth and Migration ». International Journal of Molecular Sciences 20, n^o 20 (12 octobre 2019) : 5063. http://dx.doi.org/10.3390/ijms20205063.

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Drug resistance represents a major issue in treating breast cancer, despite the identification of novel therapeutic strategies, biomarkers, and subgroups. We have previously identified the LQB-223, 11a-N-Tosyl-5-deoxi-pterocarpan, as a promising compound in sensitizing doxorubicin-resistant breast cancer cells, with little toxicity to non-neoplastic cells. Here, we investigated the mechanisms underlying LQB-223 antitumor effects in 2D and 3D models of breast cancer. MCF-7 and MDA-MB-231 cells had migration and motility profile assessed by wound-healing and phagokinetic track motility assays, respectively. Cytotoxicity in 3D conformation was evaluated by measuring spheroid size and performing acid phosphatase and gelatin migration assays. Protein expression was analyzed by immunoblotting. Our results show that LQB-223, but not doxorubicin treatment, suppressed the migratory and motility capacity of breast cancer cells. In 3D conformation, LQB-223 remarkably decreased cell viability, as well as reduced 3D culture size and migration. Mechanistically, LQB-223-mediated anticancer effects involved decreased proteins levels of XIAP, c-IAP1, and Mcl-1 chemoresistance-related proteins, but not survivin. Survivin knockdown partially potentiated LQB-223-induced cytotoxicity. Additionally, cell treatment with LQB-223 resulted in changes in the mRNA levels of epithelial-mesenchymal transition markers, suggesting that it might modulate cell plasticity. Our data demonstrate that LQB-223 impairs 3D culture growth and migration in 2D and 3D models of breast cancer exhibiting different phenotypes.

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Soler, Carles, José Á. Picazo-Bueno, Vicente Micó, Anthony Valverde, Daznia Bompart, Francisco J. Blasco, Juan G. Álvarez et Almudena García-Molina. « Effect of counting chamber depth on the accuracy of lensless microscopy for the assessment of boar sperm motility ». Reproduction, Fertility and Development 30, n^o 6 (2018) : 924. http://dx.doi.org/10.1071/rd17467.

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Sperm motility is one of the most significant parameters in the prediction of male fertility. Until now, both motility analysis using an optical microscope and computer-aided sperm analysis (CASA-Mot) entailed the use of counting chambers with a depth to 20 µm. Chamber depth significantly affects the intrinsic sperm movement, leading to an artificial motility pattern. For the first time, laser microscopy offers the possibility of avoiding this interference with sperm movement. The aims of the present study were to determine the different motility patterns observed in chambers with depths of 10, 20 and 100 µm using a new holographic approach and to compare the results obtained in the 20-µm chamber with those of the laser and optical CASA-Mot systems. The ISAS®3D-Track results showed that values for curvilinear velocity (VCL), straight line velocity, wobble and beat cross frequency were higher for the 100-µm chambers than for the 10- and 20-µm chambers. Only VCL showed a positive correlation between chambers. In addition, Bayesian analysis confirmed that the kinematic parameters observed with the 100-µm chamber were significantly different to those obtained using chambers with depths of 10 and 20 µm. When an optical analyser CASA-Mot system was used, all kinematic parameters, except VCL, were higher with ISAS®3D-Track, but were not relevant after Bayesian analysis. Finally, almost three different three-dimensional motility patterns were recognised. In conclusion, the use of the ISAS®3D-Track allows for the analysis of the natural three-dimensional pattern of sperm movement.

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Stanton, M. M., C. Trichet-Paredes et S. Sánchez. « Applications of three-dimensional (3D) printing for microswimmers and bio-hybrid robotics ». Lab on a Chip 15, n^o 7 (2015) : 1634–37. http://dx.doi.org/10.1039/c5lc90019k.

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Thèses sur le sujet "3D motility"

Godeau, Amélie. « Cyclic contractions contribute to 3D cell motility ». Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAF038/document.

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La motilité des cellules est un phénomène fondamental en biologie souvent étudié sur des surfaces planes, conditions peu physiologiques. Nous avons analysé la migration cellulaire dans une matrice cellulaire 3D contenant de la fibronectine fluorescente. Nous démontrons que les cellules y sont confinées, et déforment leur environnement de manière cyclique avec une période de ~14 min avec deux centres de contractions à l’avant et à l’arrière de la cellule qui contractent avec un déphasage de ~3.5 min. Une perturbation de ces cycles entraîne une réduction de la motilité. Par l’utilisation d’inhibiteurs spécifiques, nous avons identifié l’acto-myosine comme étant l’acteur principal de ce phénomène. En imposant des contractions-relaxations locales par ablations laser, nous avons déclenché la motilité cellulaire ce qui confirme notre hypothèse. L’ensemble de cette étude met en évidence un nouveau mécanisme fondamental de dynamique cellulaire impliqué dans le mouvement des cellules
Cell motility is an important process in Biology. It is mainly studied on 2D planar surfaces, whereas cells experience a confining 3D environment in vivo. We prepared a 3D Cell Derived Matrix (CDM) labeled with fluorescently labeled fibronectin, and strikingly cells managed to deform the matrix with specific patterns : contractions occur cyclically with two contraction centers at the front and at the back of the cell, with a period of ~14 min and a phase shift of ~3.5 min. These cycles enable cells to optimally migrate through the CDM, as perturbation of cycles led to reduced motility. Acto-myosin was established to be the driving actor of these cycles, by using specific inhibitors. We were able to trigger cell motility externally with local laser ablations, which supports this framework of two alternating contractions involved in motion. Altogether, this study reveals a new mechanism of dynamic cellular behaviour linked to cell motility

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Godeau, Amélie [Verfasser], et Albrecht [Akademischer Betreuer] Ott. « Cyclic contractions contribute to 3D cell motility / Amélie Godeau ; Betreuer : Albrecht Ott ». Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2017. http://d-nb.info/1138978833/34.

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Baker, Ryan. « IMAGING AND ANALYSIS OF LARVAL ZEBRAFISH GUT MOTILITY, AND AUTOMATED TOOLS FOR 3D MICROSCOPY ». Thesis, University of Oregon, 2018. http://hdl.handle.net/1794/23133.

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Nearly all individual members of the animal kingdom have gastrointestinal tracts which feature unique cellular compositions, geometries, and temporal dynamics. These guts are distinct enough from one another, even across siblings or even across the same individual at different points in space and time, that defining meaningful scientific representations of those features is difficult. Studying these guts is also innately challenging as it requires accessing to the insides of the enclosed 3D volumes. The work presented here describes tools and methodologies designed to address these difficulties. To investigate gut motility, we constructed a combined light sheet fluorescence and differential interference contrast microscope to obtain videos of larval zebrafish (Danio rerio) gut motility and to obtain 3D information about nearby fluorescently tagged cells. Using advanced computer vision algorithms, we quantified aspects of zebrafish gut motility which have never before been characterized, then used that information to identify the effects of different genetic, chemical, and physiological states of zebrafish gut motility. Finally, we designed and constructed an instrument for automating 3D microscopy for future studies. This dissertation includes previously published and unpublished co-authored material.

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Flewellen, James Lewis. « Digital holographic microscopy for three-dimensional studies of bacteria ». Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:94ff344b-51ec-41c5-a5f8-c579e16dccd7.

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Holography has the ability to render three-dimensional information of a recorded scene by capturing both the amplitude and phase of light incident on the recording medium. The application of digital camera technology and high-speed computing means digital holograms can be analysed numerically and novel applications can be found for this technology. This thesis explores the potential for both inline and off-axis digital holographic microscopy to study the three-dimensional swimming behaviour of bacteria. A high-magnification (225x) digital holographic microscope was designed and constructed with the ability to switch easily between inline and off-axis imaging modalities. Hardware aspects, in particular the illumination source, the choice of camera and data transfer rates, were considered. Novel strategies for off-axis holography combining dark field microscopy were designed and implemented. The localisation accuracy of the inline imaging modality was assessed by studying samples of polystyrene microspheres. The microscope is sensitive to stage drift on the order of angstroms per second and can successfully localise microspheres in dilute suspensions at least 100μm from the objective specimen plane. As a simple test of the capabilities of the microscope, the diffusion coefficient of a 0.5μm microsphere was found to be isotropic and consistent with the theoretical value. Amplitude and phase image reconstructions from the off-axis modality are demonstrated. High-magnification dark field off-axis holographic microscopy is shown to be superior to inline microscopy in localising 100nm gold nanoparticles. An artifact from our method of dark-field imaging, however, restricts the depth range to 15μm. A lower-magnification (45x) configuration of the microscope was used to study the 3D swimming behaviour of wild type Escherichia coli as a qualitative demonstration of the potential for this instrument in microbiological applications.

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Thouvenin, Olivier. « Optical 3D imaging of subcellular dynamics in biological cultures and tissues : applications to ophthalmology and neuroscience ». Thesis, Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCC169/document.

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Cette thèse a pour objectif l’étude d’un lien effectif potentiel entre la motilité cellulaire, la mécanique cellulaire, et l’activité biochimique de ces mêmes cellules. Ce couplage a été étudié dans divers systèmes biologiques, et aussi bien dans des cultures de cellules qu’à l’intérieur de tissus plus complexes. Notamment, nous avons particulièrement cherché à détecter un couplage électromécanique dans des neurones qui pourrait être impliqué dans la propagation du message nerveux.Pour ce faire, nous avons dû développer deux microscopes optiques à la sensibilité extrême. Ces microscopes se composent de deux parties principales. La première sert à détecter des mouvements axiaux plus petits que la longueur d’onde optique, soit en dessous de 100 nanomètres. La deuxième partie permet la détection d’un signal de fluorescence, offrant la possibilité de suivre l’évolution biochimique de la cellule. Avec ces deux microscopes multimodaux, il est donc possible de suivre de manière simultanée un contraste de motilité, un contraste mécanique, un contraste structurel et un contraste biochimique. Si l’un de ces systèmes est basé sur la tomographie de cohérence optique plein champ et permet de faire de telles mesures en 3-D et en profondeur dans les tissus biologiques, le second ne permet que des mesures dans des cultures de cellules, mais est bien plus robuste au bruit mécanique. Dans ce manuscrit, nous allons essentiellement décrire le développement de ces deux appareils, et préciser les contrastes auxquels ils sont sensibles spécifiquement.Nous développerons également deux des applications principales de ces microscopes que nous avons étudié dans le détail au cours de cette thèse. La première application développe l’intérêt d’un de nos microscopes pour la détection sans marquage des principaux composants cellulaires et structuraux de la cornée et de la rétine. La seconde application tend à détecter et à suivre des ondes électromécaniques dans des neurones de mammifères
This PhD project aims to explore the relationship that might exist between the dynamic motility and mechanical behavior of different biological systems and their biochemical activity. In particular,we were interested in detecting the electromechanical coupling that may happen in active neurons, and may assist in the propagation of the action potential. With this goal in mind, we have developed two highly sensitive optical microscopes that combine one modality that detects sub-wavelength axial displacements using optical phase imaging and another modality that uses a fluorescence path. Therefore, these multimodal microscopes can combine a motility, a mechanical,a structural and a biochemical contrast at the same time. One of this system is based ona multimodal combination of full-field optical coherence tomography (FF-OCT) and allows the observation of such contrast inside thick and scattering biological tissues. The other setup provides a higher displacement sensitivity, but is limited to measurements in cell cultures. In this manuscript, we mainly discuss the development of both systems and describe the various contrastst hey can reveal. Finally, we have largely used our systems to investigate diverse functions of the eye and to look for electromechanical waves in cell cultures. The thorough description of both biological applications is also provided in the manuscript

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Bresteau, Enzo. « Adhesive Clathrin Structures Support 3D Haptotaxis Through Local Force Transmission ». Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS546.

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La migration cellulaire est un processus fondamental au maintien des fonctions physiologiques de l’organisme. Elle est également centrale dans de nombreuses pathologies et entre notamment en jeu lors de la dissémination métastatique. Lorsqu’elles migrent, les cellules utilisent des structures d’adhésion afin de s’appuyer sur leur environnement. Nous avons récemment montré que les puits recouverts de clathrine, plus connus pour leur rôle dans l’endocytose, peuvent également servir de structures d’adhésion. Dans ce manuscrit, je démontre que certains ligands internalisés par la voie d’endocytose clathrine peuvent également se lier à la matrice et orienter la migration cellulaire en régulant les structures adhésives de clathrine.J’ai commencé par montrer que le collagène est associé à plus de structures de clathrine et a plus de protrusions lorsqu’il est recouvert par des ligands. J’ai ensuite montré que les cellules appliquaient plus de forces sur des fibres de collagènes décorées par des ligands et que ce surplus de force nécessite la présence de structures de clathrine. Enfin j’ai montré que les cellules suivent les ligands liés à des réseaux de collagène en 3D et que cette migration dirigée nécessite également la présence de structures de clathrine. Ce mécanisme de migration pourrait notamment permettre aux cellules de suivre des gradients de ligands liés à la matrice in vivo et ainsi de s’orienter dans l’organisme
Cell migration is a fundamental process in the development and homeostasis of multicellular organisms. It is also central to many pathologies and it is especially important for metastatic dissemination. When migrating, cells use adhesion structures to push on their substrate in order to move forward. We recently showed that clathrin coated structures, primarily known as endocytic structures, can also serve as adhesion structures. In this manuscript, I show that some ligands internalized through clathrin mediated endocytosis can also bind to the extracellular matrix and orient cell migration using adhesive clathrin structures.I first showed that ligand-decorated collagen fibers are associated with more clathrin structures and more protrusions. I then showed that cells applied more forces to the ligand-decorated collagen fibers and this extra amount of forces requires the presence of clathrin structures. Finally, I showed that cells can migrate following collagen-bound ligands in 3D, this directed migration also requiring the presence of clathrin structures. Such migration mechanism could be used by cells to follow in vivo gradient of matrix-bound ligands and thus find their way when migrating inside the body

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Howley, Stéphane. « Développement et approche de personnalisation d'un modèle numérique musculaire déformable du cou ». Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10306.

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L'objectif du travail de thèse, dans le cadre du projet Européen DEMU2NECK, a été de contribuer à développer un modèle volumique déformable et personnalisable du cou, incluant la prise en compte de la capacité d'activation musculaire. Ce modèle vise à contribuer à une meilleure compréhension des liens entre pathologie, posture et activation musculaire pour aider les cliniciens et les fabricants de dispositifs médicaux dans leurs prises de décision. Pour atteindre cet objectif la thèse a été structurée en quatre grandes tâches : après une i) synthèse bibliographique, ii) un modèle générique a été développé et validé. La troisième étape a été iii) le développement d'un modèle de muscle actif et son intégration au modèle générique. L'implémentation de la fonction contractile des muscles lors de simulations musculo-squelettiques isométriques et dynamiques de tâches fonctionnelles simples du cou, a permis de mettre en avant des efforts transverses transmis par les muscles au rachis, allant dans le sens de l'hypothèse d'une contribution à sa stabilisation. La dernière tâche a été iv) la réalisation des premières applications de personnalisation, qui ont porté sur la déformation du modèle générique sur la base de données d'imagerie obtenues sur volontaires. Des comparaisons des réponses des modèles personnalisés et du modèle générique ont mis en avant l'intérêt scientifique de l'approche de personnalisation à travers l'obtention de différences significatives de résultats
The objective of this thesis, as part of the DEMU2NECK European project, was to contribute to develop a 3D, deformable model of the neck, with contractile muscles and including the possibility of patient-specific geometric personalisation. The aim of this model is to contribute to a better understanding of the links that exist between pathologies, cervical spine posture and muscular activation in order to help clinicians and medical devices manufacturers in their decision making process. To achieve this goal, the thesis work was divided into four main tasks: after a i) bibliographic synthesis, ii) a passive generic finite element model was developed and validated. The third step consisted in iii) the development of a finite element muscle model and its integration to the generic passive model. The contractile function of the muscles was implemented during isometric and dynamic simulations of simple functional tasks of the neck. The transverse forces that were transmitted from the muscles to the cervical spine are in good agreement with the hypothesis of a contribution of these forces to the cervical spine stability. The last task covered iv) the personalisation process of the generic model. The responses of subject-specific models based on volunteers were compared with the ones obtained from the generic model. They showed significant differences and, therefore, the scientific relevance of the personalization approach

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Sharma, Yasha. « Collective cell motility in 3-dimensions : dynamics, adhesions, and emergence of heterogeneity ». Thesis, 2016. https://hdl.handle.net/2144/14625.

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Collective cell migration is ubiquitous in biology, from development to cancer; it is influenced by heterogeneous cell types, signals and matrix properties, and requires large scale regulation in space and time. Understanding how cells achieve organized collective motility is crucial to addressing cellular and tissue function and disease progression. While current two-dimensional model systems recapitulate the dynamic properties of collective cell migration, quantitative three-dimensional equivalent model systems have proved elusive. The overarching hypothesis of this work is that cell collectives are heterogeneous in nature; and that the influence of biochemical, physical, and mechanical factors combined leads to diverse physical behaviors. The central goal of this work is to establish standard tools for the understanding of 3D collective cell motility by treating individual cell-collectives as independent entities. An experimental model studies cell collectives by tracking individual cells within cell cohorts embedded in three dimensional collagen scaffolding. A computational model of 3-dimensional multi-scale self-propelled particles recreates experimental data and accounts for intercellular adhesion dynamics. A custom algorithm identifies cellular cohorts from experimental and simulated data so these may be treated as independent entities. A second custom algorithm quantifies the temporal and spatial heterogeneity of motion in cell cohorts during ‘motility events’ observed in experiments and simulations. The results show that cell-cohorts in 3D are dynamic with spatial and temporal heterogeneity; cohesive motility events can emerge without an external driving agent. Simulated cohorts are able to recreate experimental motility event signatures. Together these model systems and analytical techniques are some of the first to address collective motility of adhesive cellular cohorts in 3-dimensions.

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Chapitres de livres sur le sujet "3D motility"

Wessels, Deborah, Spencer Kuhl et David R. Soll. « 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics ». Dans Cytoskeleton Methods and Protocols, 315–35. Totowa, NJ : Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-376-3_18.

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« Cell Motility in 3D Matrices ». Dans Cell and Matrix Mechanics, 214–35. CRC Press, 2014. http://dx.doi.org/10.1201/b17612-12.

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Alio, Jorge, et Carlos Laria. « New Methods for the Analysis of Ocular Motility : 3D Video Oculography ». Dans Surgical Techniques in Ophthalmology (Strabismus Surgery), 213. Jaypee Brothers Medical Publishers (P) Ltd., 2010. http://dx.doi.org/10.5005/jp/books/11418_24.

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Nitzsche, Bert, Volker Bormuth, Corina Bräuer, Jonathon Howard, Leonid Ionov, Jacob Kerssemakers, Till Korten, Cecile Leduc, Felix Ruhnow et Stefan Diez. « Studying Kinesin Motors by Optical 3D-Nanometry in Gliding Motility Assays ». Dans Methods in Cell Biology, 247–71. Elsevier, 2010. http://dx.doi.org/10.1016/s0091-679x(10)95014-0.

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Alio, Jorge, et Carlos Laria. « New Methods for the Analysis of Ocular Motility : 3D Video-oculography ». Dans Surgical Techniques in Ophthalmology (Pediatric Ophthalmic Surgery), 307. Jaypee Brothers Medical Publishers (P) Ltd., 2011. http://dx.doi.org/10.5005/jp/books/11282_40.

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Ballav, Sangeeta, Ankita Jaywant Deshmukh, Shafina Siddiqui, Jyotirmoi Aich et Soumya Basu. « Two-Dimensional and Three-Dimensional Cell Culture and Their Applications ». Dans Cell Culture [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.100382.

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Cell culture is one of the most important and commonly used in vitro tools to comprehend various aspects of cells or tissues of a living body such as cell biology, tissue morphology, mechanism of diseases, cell signaling, drug action, cancer research and also finds its great importance in preclinical trials of various drugs. There are two major types of cell cultures that are most commonly used- two-dimensional (2D) and three-dimensional culture (3D). The former has been used since the 1900s, owing to its simplicity and low-cost maintenance as it forms a monolayer, while the latter being the advanced version and currently most worked upon. This chapter intends to provide the true meaning and significance to both cultures. It starts by making a clear distinction between the two and proceeds further to discuss their different applications in vitro. The significance of 2D culture is projected through different assays and therapeutic treatment to understand cell motility and treatment of diseases, whereas 3D culture includes different models and spheroid structures consisting of multiple layers of cells, and puts a light on its use in drug discovery and development. The chapter is concluded with a detailed account of the production of therapeutic proteins by the use of cells.

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Actes de conférences sur le sujet "3D motility"

Goh, Voon Hueh, Muhammad Amir Bin As'Ari et Lukman Hakim Bin Ismail. « 3D Convolutional Neural Networks for Sperm Motility Prediction ». Dans 2022 2nd International Conference on Intelligent Cybernetics Technology & Applications (ICICyTA). IEEE, 2022. http://dx.doi.org/10.1109/icicyta57421.2022.10037950.

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Nolte, David D., et John Turek. « Motility-Contrast Imaging : Digital Holography of Cellular Motion in 3D Tissues ». Dans Digital Holography and Three-Dimensional Imaging. Washington, D.C. : OSA, 2009. http://dx.doi.org/10.1364/dh.2009.jmb4.

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Nobe, Kazuki, Kayo Yoshimoto, Kenji Yamada et Hideya Takahashi. « 3D registration method for assessing the gastrointestinal motility using spectral reflectance estimation ». Dans Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVI, sous la direction de Tuan Vo-Dinh, Anita Mahadevan-Jansen et Warren S. Grundfest. SPIE, 2018. http://dx.doi.org/10.1117/12.2288383.

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Nolte, David D., Kwan Jeong et John J. Turek. « Digital Holographic Optical Coherence Imaging : 3D Motility Assays of the Effect of Anticancer Drugs ». Dans Photorefractive Effects, Photosensitivity, Fiber Gratings, Photonic Materials and More. Washington, D.C. : OSA, 2007. http://dx.doi.org/10.1364/pr.2007.tud2.

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Vaezi, Seyed, Gianni Orlando, Mojtaba Fazli, Gary Ward, Silvia Moreno et Shannon Quinn. « A Novel Pipeline for Cell Instance Segmentation, Tracking and Motility Classification of Toxoplasma Gondii in 3D Space ». Dans Python in Science Conference. SciPy, 2022. http://dx.doi.org/10.25080/majora-212e5952-009.

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Baker, Brendon M., Colin K. Choi, Britta Trappmann et Christopher S. Chen. « Engineered Fibrillar Extracellular Matrices for the Study of Directed Cell Migration ». Dans ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80943.

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Résumé :

The biology of cell adhesion and migration has traditionally been studied on 2D glass or plastic surfaces. While such studies have shed light on the molecular mechanisms governing these processes [1], current knowledge is limited by the dissimilarity between the flat surfaces conventionally employed and the topographically complex extracellular matrix (ECM) cells routinely navigate within the body. On ECM-coated flat surfaces, cells are presented with an unlimited expanse of adhesive ligand and can spread and migrate freely. Conversely, the availability of ligand in vivo is generally restricted to ECM structures, forcing cells to form adhesions in prescribed locations distributed through 3D space depending on the geometry and organization of the surrounding matrix [2]. These physical constraints on cell adhesion likely have profound consequences on intracellular signaling and resulting migration, and calls into question whether the mechanisms and modes of cell motility observed on flat substrates are truly reflective of the in vivo scenario [3]. The topographies of ECMs found in vivo are varied but largely fibrillar, ranging from the tightly crosslinked fibers that form the sheet-like basement membrane, to the structure of fibrin-rich clots and collagenous connective tissues. Collagen comprises approximately 25% of the human body by mass, and as such, purified collagen has served as a popular setting for the study of cell migration within a fibrillar context for many decades [4]. However, a major limitation to the use of these gels is the inability to orthogonally dictate key structural features that impact cell behavior. For example, in contrast to the large range of fiber diameters found in vivo within connective tissue resulting from hierarchical collagen assembly and multiple types of collagens [3], collagen gels are limited to fibril diameters of ∼500nm. Furthermore, recreating the structural anisotropy common to connective tissues in collagen gels is technically challenging [5]. Thus, there remains a significant need for engineered fibrillar materials that afford precise and independent control of architectural and mechanical features for application in cell biology. In this work, we develop two approaches to fabricating fibrillar ECMs in order to study cell adhesion and migration in vitro.

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Kutter, Oliver, Sonja Kirchhoff, Marina Berkovich, Maximilian Reiser et Nassir Navab. « Spatio-temporal registration in multiplane MRI acquisitions for 3D colon motiliy analysis ». Dans Medical Imaging, sous la direction de Maryellen L. Giger et Nico Karssemeijer. SPIE, 2008. http://dx.doi.org/10.1117/12.769810.

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