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Articles de revues sur le sujet « 2D-Fluorescence »

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Auteur : Grafiati
Publié le 10 mars 2023

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1

Otosu, Takuhiro, et Shoichi Yamaguchi. « Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy : Concepts and Applications ». Molecules 23, no 11 (14 novembre 2018) : 2972. http://dx.doi.org/10.3390/molecules23112972.

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We review the basic concepts and recent applications of two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS), which is the extension of fluorescence correlation spectroscopy (FCS) to analyze the correlation of fluorescence lifetime in addition to fluorescence intensity. Fluorescence lifetime is sensitive to the microenvironment and can be a “molecular ruler” when combined with FRET. Utilization of fluorescence lifetime in 2D FLCS thus enables us to quantify the inhomogeneity of the system and the interconversion dynamics among different species with a higher time resolution than other single-molecule techniques. Recent applications of 2D FLCS to various biological systems demonstrate that 2D FLCS is a unique and promising tool to quantitatively analyze the microsecond conformational dynamics of macromolecules at the single-molecule level.
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Draeger, Simon, Sebastian Roeding et Tobias Brixner. « Rapid-scan coherent 2D fluorescence spectroscopy ». Optics Express 25, no 4 (7 février 2017) : 3259. http://dx.doi.org/10.1364/oe.25.003259.

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3

Assawajaruwan, S., J. Reinalter et B. Hitzmann. « Selection Techniques for Significant Fluorescence Variables from 2D Fluorescence Spectroscopy ». Chemie Ingenieur Technik 88, no 9 (29 août 2016) : 1316. http://dx.doi.org/10.1002/cite.201650056.

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4

Pachón, Leonardo A., Andrew H. Marcus et Alán Aspuru-Guzik. « Quantum process tomography by 2D fluorescence spectroscopy ». Journal of Chemical Physics 142, no 21 (18 mai 2015) : 212442. http://dx.doi.org/10.1063/1.4919954.

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Chida, Hinako, et Keiko Tawa. « Microscopic Study on Excitation and Emission Enhancement by the Plasmon Mode on a Plasmonic Chip ». Sensors 20, no 22 (10 novembre 2020) : 6415. http://dx.doi.org/10.3390/s20226415.

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Excitation and emission enhancement by using the plasmon mode formed on a plasmonic chip was studied with a microscope and micro-spectroscope. Surface plasmon resonance wavelengths were observed on one-dimensional (1D) and two-dimensional (2D) plasmonic chips by measuring reflection and transmission spectra, and they were assigned to the plasmon modes predicted by the theoretical resonance wavelengths. The excitation and emission enhancements were evaluated using the fluorescence intensity of yellow–green fluorescence particles. The 2D grating had plasmon modes of kgx45(2) (diagonal direction with m = 2) in addition to the fundamental mode of kgx(1) (direction of a square one side) in the visible range. In epifluorescence detection, the excitation enhancement factors of kgx(2) on the 1D and 2D chips were found to be 1.3–1.4, and the emission enhancement factor of kgx45(2) on the 2D chip was 1.5–1.8, although the emission enhancement was not found on the 1D chip. Moreover, enhancement factors for the other fluorophores were also studied. The emission enhancement factor of kgx(1) was shown to depend on the fluorescence quantum yield. The emission enhancement of 2D was 1.3-fold larger than that of 1D considering all azimuth components, and the 2D pattern was shown to be advantageous for bright fluorescence microscopic observation.
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Hur, Jin, et Bo-Mi Lee. « Comparing the Heterogeneity of Copper-Binding Characteristics for Two Different-Sized Soil Humic Acid Fractions Using Fluorescence Quenching Combined with 2D-COS ». Scientific World JOURNAL 11 (2011) : 1865–76. http://dx.doi.org/10.1100/2011/640598.

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Heterogeneous distributions of copper-binding characteristics were compared for two ultrafiltered size fractions of a soil HA using fluorescence quenching combined with two-dimensional correlation spectroscopy (2D-COS). The apparent shapes of the original synchronous fluorescence spectra and the extent of the fluorescence quenching upon the addition of copper were similar for the two fractions. The stability constants calculated at their highest peaks were not significantly different. However, the 2D-COS results revealed that the fluorescence quenching behaviors were strongly affected by the associated wavelengths and the fraction's size. The spectral change preferentially occurred in the wavelength order of 467 nm → 451 nm → 357 nm for the 1–10 K fraction and of 376 nm → 464 nm for the >100 K fraction. The extent of the binding affinities exactly followed the sequential orders interpreted from the 2D-COS, and they exhibited the distinctive ranges of the logarithmic values from 5.86 to 4.91 and from 6.48 to 5.95 for the 1–10 K and the >100 K fractions, respectively. Our studies demonstrated that fluorescence quenching combined with 2D-COS could be successfully utilized to give insight into the chemical heterogeneity associated with metal-binding sites within the relatively homogeneous HA size fractions.
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7

Huang, Jiaxing. « (Invited) Seeing 2D Sheets with Fluorescence Quenching Microscopy ». ECS Meeting Abstracts MA2020-01, no 10 (1 mai 2020) : 830. http://dx.doi.org/10.1149/ma2020-0110830mtgabs.

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Kong, Zhizhi, Matthias Daab, Hitomi Yano, Haiyue Huang, Josef Breu, Takayoshi Sasaki, SonBinh T. Nguyen et Jiaxing Huang. « Visualizing Transparent 2D Sheets by Fluorescence Quenching Microscopy ». Small Methods 4, no 3 (12 février 2020) : 2000036. http://dx.doi.org/10.1002/smtd.202000036.

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9

Xue, Mao-Yun, Ai-Ping Yang, Mei-Hua Ma et Xiao-Hua Li. « The application of two-dimensional fluorescence correlation spectroscopy on the interaction between bovine serum albumin and prulifloxacin ». Spectroscopy 23, no 5-6 (2009) : 257–63. http://dx.doi.org/10.1155/2009/565173.

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The interaction between bovine serum albumin (BSA) and prulifloxacin was investigated by ultraviolet spectrophotometer (UV) and fluorescence spectroscopy in this paper. Two-dimensional (2D) correlation spectroscopy was applied to the analysis of fluorescence spectra. The results of spectroscopic measurements suggested that prulifloxacin (PL) have a strong ability to quench the intrinsic fluorescence of bovine serum albumin through static quenching procedure. Thermodynamic parameter enthalpy changes (ΔH) and entropy changes (ΔS) were calculated. Owing to the spectral resolution enhancement in 2D correlation spectroscopy, the structure change of prulifloxacin can be observed.
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10

Li, Peng, Meng-Yue Guo, Lu-Lu Gao, Xue-Mei Yin, Shuai-Liang Yang, Ran Bu et En-Qing Gao. « Photoresponsivity and antibiotic sensing properties of an entangled tris(pyridinium)-based metal–organic framework ». Dalton Transactions 49, no 22 (2020) : 7488–95. http://dx.doi.org/10.1039/d0dt00397b.

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Novel 2D → 2D entanglement affords close donor–acceptor contacts for electron transfer based photochromism and photomodulable fluorescence of a MOF, which also serves as a regenerable and sensitive luminescent sensor for nitrofuran antibiotics.
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Mizuno, T., E. Hase, T. Minamikawa, Y. Tokizane, R. Oe, H. Koresawa, H. Yamamoto et T. Yasui. « Full-field fluorescence lifetime dual-comb microscopy using spectral mapping and frequency multiplexing of dual-comb optical beats ». Science Advances 7, no 1 (janvier 2021) : eabd2102. http://dx.doi.org/10.1126/sciadv.abd2102.

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Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust to photobleaching. However, since most FLIMs are based on point-to-point measurements, mechanical scanning of a focal spot is needed for forming an image, which hampers rapid imaging. Here, we demonstrate scan-less full-field FLIM based on a one-to-one correspondence between two-dimensional (2D) image pixels and frequency-multiplexed radio frequency (RF) signals. A vast number of dual-comb optical beats between dual optical frequency combs are effectively adopted for 2D spectral mapping and high-density frequency multiplexing in the RF region. Bimodal images of fluorescence amplitude and lifetime are obtained with high quantitativeness from amplitude and phase spectra of fluorescence RF comb modes without the need for mechanical scanning. The parallelized FLIM will be useful for rapid quantitative fluorescence imaging in life science.
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Cascarano, Pasquale, Maria Colomba Comes, Andrea Sebastiani, Arianna Mencattini, Elena Loli Piccolomini et Eugenio Martinelli. « DeepCEL0 for 2D single-molecule localization in fluorescence microscopy ». Bioinformatics 38, no 5 (2 décembre 2021) : 1411–19. http://dx.doi.org/10.1093/bioinformatics/btab808.

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Abstract Motivation In fluorescence microscopy, single-molecule localization microscopy (SMLM) techniques aim at localizing with high-precision high-density fluorescent molecules by stochastically activating and imaging small subsets of blinking emitters. Super resolution plays an important role in this field since it allows to go beyond the intrinsic light diffraction limit. Results In this work, we propose a deep learning-based algorithm for precise molecule localization of high-density frames acquired by SMLM techniques whose ℓ2-based loss function is regularized by non-negative and ℓ0-based constraints. The ℓ0 is relaxed through its continuous exact ℓ0 (CEL0) counterpart. The arising approach, named DeepCEL0, is parameter-free, more flexible, faster and provides more precise molecule localization maps if compared to the other state-of-the-art methods. We validate our approach on both simulated and real fluorescence microscopy data. Availability and implementation DeepCEL0 code is freely accessible at https://github.com/sedaboni/DeepCEL0.
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Fransen, M. « 1- and 2D Detectors and sample fluorescence in XRD ». Acta Crystallographica Section A Foundations of Crystallography 61, a1 (23 août 2005) : c146. http://dx.doi.org/10.1107/s0108767305093803.

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14

Zhang, Zhen-Yu, Hai-Yu Wang, Jiang-Lin Du, Xu-Lin Zhang, Ya-Wei Hao, Qi-Dai Chen et Hong-Bo Sun. « Surface Plasmon-Modulated Fluorescence on 2D Metallic Silver Gratings ». IEEE Photonics Technology Letters 27, no 8 (15 avril 2015) : 821–23. http://dx.doi.org/10.1109/lpt.2015.2392431.

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15

SIMANTIRAKI, MARIA, ROSY FAVICCHIO, STELIOS PSYCHARAKIS, GIANNIS ZACHARAKIS et JORGE RIPOLL. « MULTISPECTRAL UNMIXING OF FLUORESCENCE MOLECULAR TOMOGRAPHY DATA ». Journal of Innovative Optical Health Sciences 02, no 04 (octobre 2009) : 353–64. http://dx.doi.org/10.1142/s1793545809000656.

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Even though multispectral imaging is considered very significant in biological imaging, it is only commonly used in microscopy in a 2D approach. Here, we present a Fluorescence Molecular Tomography system capable of recording simultaneously tomographic data at several spectral windows, enabling multispectral tomography. 3D reconstructed data from several spectral windows is used to construct a linear unmixing algorithm for multispectral deconvolution of overlapping fluorescence signals. The method is applied on tomographic 3D fluorescence concentration maps in tissue-mimicking phantoms, yielding absolute quantification of the concentration of each individual fluorophore. Results are compared to the case when unmixing is performed in the raw 2D data instead of the reconstructed 3D concentration map, showing greater accuracy when unmixing algorithms are applied in the reconstructed data. Both the reflection and transmission geometries are considered.
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16

Sowrirajan, Chandrasekaran, Sameena Yousuf et Israel V. M. V. Enoch. « The Unusual Fluorescence Quenching of Coumarin 314 by β-Cyclodextrin and the Effect of β-Cyclodextrin on its Binding with Calf Thymus DNA ». Australian Journal of Chemistry 67, no 2 (2014) : 256. http://dx.doi.org/10.1071/ch13364.

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This paper discusses the binding of a laser dye, Coumarin 314 with β-cyclodextrin, studied mainly by UV-visible spectroscopy, 2D rotating-frame nuclear Overhauser effect spectroscopy (ROESY), steady-state spectroscopy and time-resolved fluorescence spectroscopy. The role of β-cyclodextrin on the binding of Coumarin 314 with calf thymus DNA was investigated. Coumarin 314 shows a hyperchromic shift of absorption and a quenching of fluorescence due to binding with β-cyclodextrin. The fluorescence quenching is non-linear and the reason for the non-linearity is discussed. The unusual fluorescence quenching on Coumarin 314–β-cyclodextrin binding is rationalised from the effect of acidity on absorption, fluorescence, and molecular modelling studies. Additional proof for the mode of binding is given by 2D ROESY. The capped and exposed portions of the Coumarin 314 molecule in the Coumarin 314–β-cyclodextrin complex when binding with calf thymus DNA were visualised based on spectral and molecular modelling studies.
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17

Li, Rui, Yajun Zhang, Xuefeng Xu, Yi Zhou, Maodu Chen et Mengtao Sun. « Optical characterizations of two-dimensional materials using nonlinear optical microscopies of CARS, TPEF, and SHG ». Nanophotonics 7, no 5 (24 mai 2018) : 873–81. http://dx.doi.org/10.1515/nanoph-2018-0002.

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AbstractIn this paper, we employ the nonlinear optical microscopies of coherent anti-Stokes Raman scattering spectroscopy, two-photon excitation fluorescence, and second harmonic generation to characterize the properties of two-dimensional (2D) materials. With these nonlinear optical microscopy methods, we can not only clearly observe the surface topography of 2D materials but also reveal the quality of 2D materials. These nonlinear optical microscopies offer great potential for characterization of the properties of 2D materials.
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18

Xie, Donghao, Ding-Kun Ji, Yue Zhang, Jun Cao, Hu Zheng, Lin Liu, Yi Zang et al. « Targeted fluorescence imaging enhanced by 2D materials : a comparison between 2D MoS2 and graphene oxide ». Chemical Communications 52, no 60 (2016) : 9418–21. http://dx.doi.org/10.1039/c6cc04687h.

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Vittala, Sandeepa Kulala, Sajena Kanangat Saraswathi, Anjali Bindu Ramesan et Joshy Joseph. « Nanosheets and 2D-nanonetworks by mutually assisted self-assembly of fullerene clusters and DNA three-way junctions ». Nanoscale Advances 1, no 10 (2019) : 4158–65. http://dx.doi.org/10.1039/c9na00485h.

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Self-assembly of F-An nanoclusters with 3WJ-DNA and 3WJ-OH offers nanosheets and entangled 2D-nanonetworks, respectively. 3WJ-OH/F-An in the presence of AgNCs shows enhanced fluorescence (∼40%) due to its stabilization in the 2D-nanonetworks.
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20

Tang, Long, Huan-Huan Wang, Yu-Hao Fu, Yi-Tong Wang, JiJiang Wang et XiangYang Hou. « Three cobalt-based coordination polymers with tripodal carboxylate and imidazole-containing ligands : syntheses, structures, properties and DFT studies ». RSC Advances 9, no 66 (2019) : 38902–11. http://dx.doi.org/10.1039/c9ra07737e.

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The tripodal carboxylate ligand can be employed in Co(ii) salt/imidazole-containing ligand systems to generate 1D chain, 2D layer, and 2D to 3D network, and the fluorescence properties of 1–3 and magnetic behavior of 1 and 2 have been investigated.
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Li, Donghai, Matthias Nuss, Sebastian Goetz, Verena Kolb, Jens Pflaum, Chiara Trovatello, Giulio Cerullo et Tobias Brixner. « Spatially resolved coherent 2D fluorescence spectroscopy within a high-NA microscope ». EPJ Web of Conferences 205 (2019) : 03014. http://dx.doi.org/10.1051/epjconf/201920503014.

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We have developed coherent two-dimensional (2D) fluorescence micro-spectroscopy which probes the nonlinear optical response at surfaces via fluorescence detection with sub-micron spatial resolution. This enables the investigation of microscopic variations in laterally heterogeneous film samples which are of interests for sub-wavelength opto-electronic devices.
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Sharma, Nallin, et Hui-Fen Wu. « The emergence of red fluorescence from two-dimensional nitrogenated-stanene oxide nanosheets ». Nanoscale 12, no 19 (2020) : 10505–10. http://dx.doi.org/10.1039/d0nr02292f.

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Wright, Penny A., Helen F. Boyd, Richard C. Bethell, Michael Busch, Phillip Gribbon, Joachim Kraemer, Eloisa Lopez-Calle, Thomas H. Mander, Dirk Winkler et Neil Benson. « Development of a 1-μl Scale Assay for Mitogen-Activated Kinase Kinase 7 Using 2-D Fluorescence Intensity Distribution Analysis Anisotropy ». Journal of Biomolecular Screening 7, no 5 (octobre 2002) : 419–28. http://dx.doi.org/10.1177/108705702237673.

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This paper describes the development of a robust, miniaturizable, and quantitative fluorescence-based assay for mitogen-activated protein kinase kinase 7 (MKK7). As a first step, the basic steady-state kinetics of the MKK7-catalyzed phosphorylation of c-Jun N-terminal kinases (JNKs) 1, 2, and 3 were defined using standard radiometric methods. Subsequently, the authors found that in addition to the holo JNKs, a series of novel small peptides (based on the region around the JNK phosphorylation site) are also substrates, provided that these were prephosphorylated on the Y residue of the TPY motif. One of these peptide substrates was used in the development of a fluorescence polarization-based assay using an antibody as a sensor. The assay was successfully miniaturized for use with conventional fluorescence polarization (FP) reader technology in 8.5 μl and on the single μl scale using Evotec proprietary 2-dimensional fluorescence intensity distribution analysis (2D-FIDA) anisotropy and liquid handling technology. The steady-state kinetic parameters derived using the FP or 2D-FIDA anisotropy format assays correlated well with those generated using a radiometric assay. Moreover, the quantitative sensitivity to known inhibitors was maintained independent of the format and assay volume. In addition, the authors found that the 2D-FIDA anisotropy assay exhibited superior performance statistics (typical Z' = ~0.5) relative to conventional FP (typical Z' = 0.3) and yielded the additional benefit of order-of-magnitude savings in terms of reagent costs. The 2D-FIDA anisotropy assay was used to carry out a successful high-throughput screening in 1-μl final volume against company file compounds.
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Guo, Xu-jing, Yun-zhen Li, Yan-hong Feng et Dong-hai Yuan. « Using fluorescence quenching combined with two-dimensional correlation fluorescence spectroscopy to characterise the binding-site heterogeneity of dissolved organic matter with copper and mercury in lake sediments ». Environmental Chemistry 14, no 2 (2017) : 91. http://dx.doi.org/10.1071/en16135.

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Environmental contextDissolved organic matter (DOM) plays an important role in influencing the migration and ultimate fate of metals. Different sources of pollution resulted in changes in the structure of sediment DOM in Lake Wuliangsuhai. We investigate the binding properties of DOM with CuII and HgII using fluorescence quenching combined with two-dimensional correlation spectroscopy (2D-COS) in order to demonstrate the influence of different sources of DOM on metals. AbstractDissolved organic matter (DOM) was collected from three sampling sites (L1, L2 and L3) in Lake Wuliangsuhai. L1 received upstream industrial wastewater and domestic sewage. L2 had suffered from agricultural non-point source pollution. L3 was situated in the lake outlet area. The complexation of DOM with CuII and HgII was investigated based on fluorescence quenching of the synchronous fluorescence spectra on the addition of CuII and on two-dimensional correlation spectroscopy (2D-COS). The synchronous and asynchronous maps derived from 2D-COS provided a clear picture of the heterogeneous distribution of CuII and HgII binding sites, which was not readily detected using only the synchronous fluorescence spectra. CuII and HgII complexation was stronger at shorter wavelengths than at longer wavelengths. Moreover, fluorescence quenching also occurred intensely in the fulvic-like regions (363nm for DOM-Cu in L2 and 365nm for DOM-Hg in L1). The logarithms of the stability constants (log KM) ranged from 4.42 to 6.23, from 4.75 to 4.86, and from 4.80 to 5.73 for DOM-Cu in L1, L2 and L3, respectively, depending on the wavelength. DOM at the longer wavelengths exhibited a higher log KM than that at the shorter wavelengths, and the f values in the protein-like region were clearly high. These results suggest that the influence of the structural and chemical properties of DOM on CuII binding may differ for DOM from different sources. The combined approach of fluorescence quenching and 2D-COS could be applied as a tool for evaluating the metal binding site heterogeneity of DOM.
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Huang, Jiaxing. « (Invited) Fluorescence Quenching Microscopy for Imaging 2D Materials : An Update ». ECS Meeting Abstracts MA2021-01, no 14 (30 mai 2021) : 671. http://dx.doi.org/10.1149/ma2021-0114671mtgabs.

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Barnat, E. V., et B. R. Weatherford. « 2D laser-collision induced fluorescence in low-pressure argon discharges ». Plasma Sources Science and Technology 24, no 5 (25 septembre 2015) : 055024. http://dx.doi.org/10.1088/0963-0252/24/5/055024.

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Kumar, Sunil, Dean Wilding, Markus B. Sikkel, Alexander R. Lyon, Ken T. MacLeod et Chris Dunsby. « High-speed 2D and 3D fluorescence microscopy of cardiac myocytes ». Optics Express 19, no 15 (6 juillet 2011) : 13839. http://dx.doi.org/10.1364/oe.19.013839.

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Koike, Masaki, Isao H. Suzuki, Sigeru Nakae et Ken-ichi Hasegawa. « A 2D imaging detector system for X-ray fluorescence analysis ». Nuclear Instruments and Methods in Physics Research Section A : Accelerators, Spectrometers, Detectors and Associated Equipment 299, no 1-3 (décembre 1990) : 567–70. http://dx.doi.org/10.1016/0168-9002(90)90845-w.

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Cueva, Evelyn, Matias Courdurier, Axel Osses, Victor Castañeda, Benjamin Palacios et Steffen Härtel. « Mathematical modeling for 2D light-sheet fluorescence microscopy image reconstruction ». Inverse Problems 36, no 7 (1 juillet 2020) : 075005. http://dx.doi.org/10.1088/1361-6420/ab80d8.

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Malý, Pavel, Julian Lüttig, Stefan Mueller, Maximilian H. Schreck, Christoph Lambert et Tobias Brixner. « Coherently and fluorescence-detected two-dimensional electronic spectroscopy : direct comparison on squaraine dimers ». Physical Chemistry Chemical Physics 22, no 37 (2020) : 21222–37. http://dx.doi.org/10.1039/d0cp03218b.

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Yao, Cheng-You, Bo Li et Zhen Qiu. « 2D Au-Coated Resonant MEMS Scanner for NIR Fluorescence Intraoperative Confocal Microscope ». Micromachines 10, no 5 (30 avril 2019) : 295. http://dx.doi.org/10.3390/mi10050295.

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The electrostatic MEMS scanner plays an important role in the miniaturization of the microscopic imaging system. We have developed a new two-dimensional (2D) parametrically-resonant MEMS scanner with patterned Au coating (>90% reflectivity at an NIR 785-nm wavelength), for a near-infrared (NIR) fluorescence intraoperative confocal microscopic imaging system with a compact form factor. A silicon-on-insulator (SOI)-wafer based dicing-free microfabrication process has been developed for mass-production with high yield. Based on an in-plane comb-drive configuration, the resonant MEMS scanner performs 2D Lissajous pattern scanning with a large mechanical scanning angle (MSA, ±4°) on each axis at low driving voltage (36 V). A large field-of-view (FOV) has been achieved by using a post-objective scanning architecture of the confocal microscope. We have integrated the new MEMS scanner into a custom-made NIR fluorescence intraoperative confocal microscope with an outer diameter of 5.5 mm at its distal-end. Axial scanning has been achieved by using a piezoelectric actuator-based driving mechanism. We have successfully demonstrated ex vivo 2D imaging on human tissue specimens with up to five frames/s. The 2D resonant MEMS scanner can potentially be utilized for many applications, including multiphoton microendoscopy and wide-field endoscopy.
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Pajuelo-Corral, Oier, Antonio Rodríguez-Diéguez, Jose A. García, Eider San Sebastián, Jose M. Seco et Javier Cepeda. « Chiral coordination polymers based on d10 metals and 2-aminonicotinate with blue fluorescent/green phosphorescent anisotropic emissions ». Dalton Transactions 47, no 26 (2018) : 8746–54. http://dx.doi.org/10.1039/c8dt01159a.

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Babor, Majharulislam, Olivier Paquet-Durand, Christoph Berg, Jochen Büchs et Bernd Hitzmann. « Online Process State Estimation for Hansenula Polymorpha Cultivation with 2D Fluorescence Spectra-Based Chemometric Model Calibrated from a Theoretical Model in Place of Offline Measurements ». Fermentation 9, no 2 (21 janvier 2023) : 95. http://dx.doi.org/10.3390/fermentation9020095.

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The use of 2D fluorescence spectra is a powerful, instantaneous, and highly accurate method to estimate the state of bioprocesses. The conventional approach for calibrating a chemometric model from raw spectra needs a large number of offline measurements from numerous runs, which is tedious, time-consuming, and error-prone. In addition, many process variables lack direct signal responses, which forces chemometric models to make predictions based on indirect responses. In order to predict glycerol and biomass concentrations online in batch cultivation of Hansenula polymorpha, this study substituted offline measurements with simulated values. The only data from cultivations needed to generate the chemometric model were the 2D fluorescence spectra, with the presumption that they contain sufficient information to characterize the process state at a measurement point. The remainder of the evaluation was carried out with the aid of a mathematical process model that describes the theoretical interferences between process variables in the system. It is shown that the process model parameters, including microbial growth rate, the yield of biomass from glycerol, and lag time can be determined from only the spectra by employing a model-based calibration (MBC) approach. The prediction errors for glycerol and biomass concentrations were 8.6% and 5.7%, respectively. An improved model-based calibration (IMBC) approach is presented that calibrates a chemometric model for only biomass. Biomass was predicted from a 2D fluorescence spectrum in new cultivations, and glycerol concentration was estimated from the process model utilizing predicted biomass as an input. By using this method, the prediction errors for glycerol and biomass were reduced to 5.2% and 4.7%, respectively. The findings indicate that model-based calibration, which can be carried out with only 2D fluorescence spectra gathered from prior runs, is an effective method for estimating the process state online.
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Hu, Guangqi, Xiaokai Xu, Bingfu Lei, Jianle Zhuang, Xuejie Zhang, Haoran Zhang, Chaofan Hu, Xiaotang Liu, Yingji He et Yingliang Liu. « Self-formed C-dot-based 2D polysiloxane with high photoluminescence quantum yield and stability ». Nanoscale 12, no 19 (2020) : 10771–80. http://dx.doi.org/10.1039/d0nr00743a.

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Liu, Kangzhi, Yu Tian, Qing Li, Xiang-Yun Du, Jing Zhang, Cai-Feng Wang et Su Chen. « Microfluidic printing directing photonic crystal bead 2D code patterns ». Journal of Materials Chemistry C 6, no 9 (2018) : 2336–41. http://dx.doi.org/10.1039/c7tc05355j.

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We developed a new microfluidic printing technology for the fabrication of multi-signal 2D code patterns with structural colors and fluorescence properties, which may have potential applications in anti-counterfeiting and optoelectronic fields.
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Feng, Qiushi, Jia Shi, Weiqiang Yang, Weiheng Zhong, Yuanzheng Li, Heyu Chen, Weizhen Liu, Haiyang Xu, Xinfeng Liu et Yichun Liu. « Engineering fluorescence intensity and electron concentration of monolayer MoS2 by forming heterostructures with semiconductor dots ». Nanoscale 11, no 14 (2019) : 6544–51. http://dx.doi.org/10.1039/c8nr08209j.

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Xu, Shun-Qi, Xiang Zhang, Cheng-Bin Nie, Zhong-Fu Pang, Xiao-Na Xu et Xin Zhao. « The construction of a two-dimensional supramolecular organic framework with parallelogram pores and stepwise fluorescence enhancement ». Chemical Communications 51, no 91 (2015) : 16417–20. http://dx.doi.org/10.1039/c5cc05875a.

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Zabadaj, Marcin, et Patrycja Ciosek-Skibińska. « Quantum Dots—Assisted 2D Fluorescence for Pattern Based Sensing of Amino Acids, Oligopeptides and Neurotransmitters ». Sensors 19, no 17 (22 août 2019) : 3655. http://dx.doi.org/10.3390/s19173655.

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Quantum dots (QDs) are very attractive nanomaterials for analytical chemistry, due to high photostability, large surface area featuring numerous ways of bioconjugation with biomolecules, usually high quantum yield and long decay times. Their broad absorption spectra and narrow, sharp emission spectra of size-tunable fluorescence make them ideal tools for pattern-based sensing. However, almost always they are applied for specific sensing with zero-dimensional (0D) signal reporting (only peak heights or peak shifts are considered), without taking advantage of greater amount of information hidden in 1D signal (emission spectra), or huge amount of information hidden in 2D fluorescence maps (Excitation-Emission Matrixes, EEMs). Therefore, in this work we propose opposite strategy—non-specific interactions of QDs, which are usually avoided and regarded as their disadvantage, were exploited here for 2D fluorescence fingerprinting. Analyte-specific multivariate fluorescence response of QDs is decoded with the use of Partial Least Squares—Discriminant Analysis. Even though only one type of QDs is studied, the proposed pattern-based method enables to obtain satisfactory accuracy for all studied compounds—various neurotransmitters, amino-acids and oligopeptides. This is a proof of principle of the possibility of the identification of various bioanalytes by such fluorescence fingerprinting with the use of QDs.
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Georgiev, Nikola, et Marcus Aldén. « Two-Dimensional Imaging of Flame Species Using Two-Photon Laser-Induced Fluorescence ». Applied Spectroscopy 51, no 8 (août 1997) : 1229–37. http://dx.doi.org/10.1366/0003702971941809.

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The potential for two-dimensional visualization of combustion species by using two-photon laser-induced fluorescence (LIF) has been investigated. The technique was applied for two-dimensional (2D) imaging of carbon monoxide, ammonia, oxygen, and hydrogen atoms in flames. Approaches for compensating the signal intensity for the quadratic laser intensity dependence in two-photon imaging are discussed. For the case of CO and H atom visualization, a potential problem is the interference from nonresonantly excited C2, whose emission spectrally and spatially coincides with the fluorescence from CO. Different strategies for elimination of the C2 emission were investigated. It was found out that the emissions from CO and C2 can be separated in time. For the case of the oxygen atoms, it was observed that the relation between the intensities of the fluorescence signals at 845 and 777 nm changes with the equivalence ratio of the investigated flame. An attempt to estimate the 2D detection limit for these species in flames is also made.
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Karki, Khadga J., Junsheng Chen, Atsunori Sakurai, Qi Shi, Alastair T. Gardiner, Oliver Kühn, Richard J. Cogdell et Tönu Pullerits. « Before Förster. Initial excitation in photosynthetic light harvesting ». Chemical Science 10, no 34 (2019) : 7923–28. http://dx.doi.org/10.1039/c9sc01888c.

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Fluorescence detected double quantum coherence 2D spectroscopy reveals strong correlation between weakly coupled pigment pools directly after absorption of light before the Förster transfer regime sets in.
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Wolf, G., J. S. Almeida, J. G. Crespo et M. A. M. Reis. « Monitoring of biofilm reactors using natural fluorescence fingerprints ». Water Science and Technology 47, no 5 (1 mars 2003) : 161–67. http://dx.doi.org/10.2166/wst.2003.0309.

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Natural fluorescence as a method to monitor biofilm processes was studied, using the example of an extractive membrane bioreactor for the degradation of 3-chloro-4 methylaniline and 1,2-dichloroethane. Non-invasive, on-line, in-situ 2D fluorometry monitoring was employed to elicit biofilm process status. The fluorescence fingerprints were deconvoluted in a pattern recognition approach using artificial neural networks (ANN) through association with key process performance parameters.
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Muhammad, Nadeem, Fenglian Wang, Qamar Subhani, Qiming Zhao, Muhammad Abdul Qadir, Hairong Cui et Yan Zhu. « Comprehensive two-dimensional ion chromatography (2D-IC) coupled to a post-column photochemical fluorescence detection system for determination of neonicotinoids (imidacloprid and clothianidin) in food samples ». RSC Advances 8, no 17 (2018) : 9277–86. http://dx.doi.org/10.1039/c7ra12555k.

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Palmieri, Valentina, Marta Barba, Lorena Di Pietro, Claudio Conti, Marco De Spirito, Wanda Lattanzi et Massimiliano Papi. « Graphene Oxide Induced Osteogenesis Quantification by In-Situ 2D-Fluorescence Spectroscopy ». International Journal of Molecular Sciences 19, no 11 (26 octobre 2018) : 3336. http://dx.doi.org/10.3390/ijms19113336.

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Graphene and graphene oxide can promote the adhesion, growth and differentiation of mesenchymal stem cells. Further, graphene surface coatings accelerate the differentiation of human mesenchymal stem cells acting as osteogenic inducers. Quantification of the osteogenic induction is conventionally performed with Alizarin Red S (ARS), an anthraquinone derivative used to identify calcium deposits in tissue sections and cell cultures. The ARS staining is quite versatile because the dye forms an Alizarin Red S–calcium complex that can be extracted from the stained monolayer of cells and readily assayed by absorbance measurements. Direct visualization of stained deposits is also feasible; however, an in-situ visualization and quantification of deposits is possible only on transparent supports and not on thick opaque materials like ceramics and graphene composites that are well-known inducers of osteogenesis. In this manuscript, the shape of the 2D-fluorescence spectra of the ARS-calcium complex is used to develop a method to detect and monitor the in-situ differentiation process occurring during the osteogenic induction mediated by opaque graphene oxide surfaces.
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Galinha, Claudia F., Carla Portugal, Gilda Carvalho, Giuseppe Guglielmi, Daniele Chiarani, Gianni Andreottola, Rui Oliveira, Maria A. M. Reis et João G. Crespo. « Monitoring of Membrane Bioreactors for Wastewater Treatment Using 2D-Fluorescence Spectroscopy ». Proceedings of the Water Environment Federation 2009, no 10 (1 janvier 2009) : 5629–32. http://dx.doi.org/10.2175/193864709793952873.

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Solle, D., D. Geissler, E. Stark, T. Scheper et B. Hitzmann. « Chemometric Modelling based on 2D-Fluorescence Spectra without a Calibration Measurement ». Bioinformatics 19, no 2 (22 janvier 2003) : 173–77. http://dx.doi.org/10.1093/bioinformatics/19.2.173.

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Zhao, Yingjie, Richard H. M. Bernitzky, Max J. Kory, Gregor Hofer, Johan Hofkens et A. Dieter Schlüter. « Decorating the Edges of a 2D Polymer with a Fluorescence Label ». Journal of the American Chemical Society 138, no 28 (11 juillet 2016) : 8976–81. http://dx.doi.org/10.1021/jacs.6b05456.

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Parsa, Fatemeh, Massomeh Ghorbanloo, Ali Morsali, Jun Wang, Peter C. Junk et Pascal Retailleau. « Azobenzene based 2D-MOF for high selective quinone fluorescence sensing performance ». Inorganica Chimica Acta 510 (septembre 2020) : 119699. http://dx.doi.org/10.1016/j.ica.2020.119699.

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Ranzan, Cassiano, Lucas Ranzan, Luciane F. Trierweiler et Jorge O. Trierweiler. « Sulfur Determination in Diesel using 2D Fluorescence Spectroscopy and Linear Models ». IFAC-PapersOnLine 48, no 8 (2015) : 415–20. http://dx.doi.org/10.1016/j.ifacol.2015.09.003.

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Özden, Ayberk, Hüseyin Şar, Aydan Yeltik, Büşra Madenoğlu, Cem Sevik, Feridun Ay et Nihan Kosku Perkgöz. « CVD grown 2D MoS2layers : A photoluminescence and fluorescence lifetime imaging study ». physica status solidi (RRL) - Rapid Research Letters 10, no 11 (5 septembre 2016) : 792–96. http://dx.doi.org/10.1002/pssr.201600204.

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Galinha, Claudia F., et João G. Crespo. « Development and Implementation of MBR Monitoring : Use of 2D Fluorescence Spectroscopy ». Membranes 12, no 12 (2 décembre 2022) : 1218. http://dx.doi.org/10.3390/membranes12121218.

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The monitoring of a membrane bioreactor (MBR) requires the assessment of both biological and membrane performance. Additionally, the development of membrane fouling and the requirements for frequent membrane cleaning are still major concerns during MBR operation, requiring tight monitoring and system characterization. Transmembrane pressure is usually monitored online and allows following the evolution of membrane performance. However, it does not allow distinguishing the fouling mechanisms occurring in the system or predicting the future behavior of the membrane. The assessment of the biological medium requires manual sampling, and the analyses involve several steps that are labor-intensive, with low temporal resolution, preventing real-time monitoring. Two-dimensional fluorescence spectroscopy is a comprehensive technique, able to assess the system status at real-time without disturbing the biological system. It provides large sets of data (system fingerprints) from which meaningful information can be extracted. Nevertheless, mathematical data analysis (such as machine learning) is essential to properly extract the information contained in fluorescence spectra and correlate it with operating and performance parameters. The potential of 2D fluorescence spectroscopy as a process monitoring tool for MBRs is, therefore, discussed in the present work in view of the actual knowledge and the authors’ own experience in this field.
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