Bibliographies thématiques / 2D-Fluorescence

Littérature scientifique sur le sujet « 2D-Fluorescence »

Auteur : Grafiati
Publié le 10 mars 2023

Créez une référence correcte selon les styles APA, MLA, Chicago, Harvard et plusieurs autres

Choisissez une source :

Sommaire

Consultez les listes thématiques d’articles de revues, de livres, de thèses, de rapports de conférences et d’autres sources académiques sur le sujet « 2D-Fluorescence ».

À côté de chaque source dans la liste de références il y a un bouton « Ajouter à la bibliographie ». Cliquez sur ce bouton, et nous générerons automatiquement la référence bibliographique pour la source choisie selon votre style de citation préféré : APA, MLA, Harvard, Vancouver, Chicago, etc.

Vous pouvez aussi télécharger le texte intégral de la publication scolaire au format pdf et consulter son résumé en ligne lorsque ces informations sont inclues dans les métadonnées.

Articles de revues sur le sujet "2D-Fluorescence"

1

Otosu, Takuhiro, et Shoichi Yamaguchi. « Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy : Concepts and Applications ». Molecules 23, no 11 (14 novembre 2018) : 2972. http://dx.doi.org/10.3390/molecules23112972.

Texte intégral
Résumé :
We review the basic concepts and recent applications of two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS), which is the extension of fluorescence correlation spectroscopy (FCS) to analyze the correlation of fluorescence lifetime in addition to fluorescence intensity. Fluorescence lifetime is sensitive to the microenvironment and can be a “molecular ruler” when combined with FRET. Utilization of fluorescence lifetime in 2D FLCS thus enables us to quantify the inhomogeneity of the system and the interconversion dynamics among different species with a higher time resolution than other single-molecule techniques. Recent applications of 2D FLCS to various biological systems demonstrate that 2D FLCS is a unique and promising tool to quantitatively analyze the microsecond conformational dynamics of macromolecules at the single-molecule level.
Styles APA, Harvard, Vancouver, ISO, etc.
2

Draeger, Simon, Sebastian Roeding et Tobias Brixner. « Rapid-scan coherent 2D fluorescence spectroscopy ». Optics Express 25, no 4 (7 février 2017) : 3259. http://dx.doi.org/10.1364/oe.25.003259.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
3

Assawajaruwan, S., J. Reinalter et B. Hitzmann. « Selection Techniques for Significant Fluorescence Variables from 2D Fluorescence Spectroscopy ». Chemie Ingenieur Technik 88, no 9 (29 août 2016) : 1316. http://dx.doi.org/10.1002/cite.201650056.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
4

Pachón, Leonardo A., Andrew H. Marcus et Alán Aspuru-Guzik. « Quantum process tomography by 2D fluorescence spectroscopy ». Journal of Chemical Physics 142, no 21 (18 mai 2015) : 212442. http://dx.doi.org/10.1063/1.4919954.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
5

Chida, Hinako, et Keiko Tawa. « Microscopic Study on Excitation and Emission Enhancement by the Plasmon Mode on a Plasmonic Chip ». Sensors 20, no 22 (10 novembre 2020) : 6415. http://dx.doi.org/10.3390/s20226415.

Texte intégral
Résumé :
Excitation and emission enhancement by using the plasmon mode formed on a plasmonic chip was studied with a microscope and micro-spectroscope. Surface plasmon resonance wavelengths were observed on one-dimensional (1D) and two-dimensional (2D) plasmonic chips by measuring reflection and transmission spectra, and they were assigned to the plasmon modes predicted by the theoretical resonance wavelengths. The excitation and emission enhancements were evaluated using the fluorescence intensity of yellow–green fluorescence particles. The 2D grating had plasmon modes of kgx45(2) (diagonal direction with m = 2) in addition to the fundamental mode of kgx(1) (direction of a square one side) in the visible range. In epifluorescence detection, the excitation enhancement factors of kgx(2) on the 1D and 2D chips were found to be 1.3–1.4, and the emission enhancement factor of kgx45(2) on the 2D chip was 1.5–1.8, although the emission enhancement was not found on the 1D chip. Moreover, enhancement factors for the other fluorophores were also studied. The emission enhancement factor of kgx(1) was shown to depend on the fluorescence quantum yield. The emission enhancement of 2D was 1.3-fold larger than that of 1D considering all azimuth components, and the 2D pattern was shown to be advantageous for bright fluorescence microscopic observation.
Styles APA, Harvard, Vancouver, ISO, etc.
6

Hur, Jin, et Bo-Mi Lee. « Comparing the Heterogeneity of Copper-Binding Characteristics for Two Different-Sized Soil Humic Acid Fractions Using Fluorescence Quenching Combined with 2D-COS ». Scientific World JOURNAL 11 (2011) : 1865–76. http://dx.doi.org/10.1100/2011/640598.

Texte intégral
Résumé :
Heterogeneous distributions of copper-binding characteristics were compared for two ultrafiltered size fractions of a soil HA using fluorescence quenching combined with two-dimensional correlation spectroscopy (2D-COS). The apparent shapes of the original synchronous fluorescence spectra and the extent of the fluorescence quenching upon the addition of copper were similar for the two fractions. The stability constants calculated at their highest peaks were not significantly different. However, the 2D-COS results revealed that the fluorescence quenching behaviors were strongly affected by the associated wavelengths and the fraction's size. The spectral change preferentially occurred in the wavelength order of 467 nm → 451 nm → 357 nm for the 1–10 K fraction and of 376 nm → 464 nm for the >100 K fraction. The extent of the binding affinities exactly followed the sequential orders interpreted from the 2D-COS, and they exhibited the distinctive ranges of the logarithmic values from 5.86 to 4.91 and from 6.48 to 5.95 for the 1–10 K and the >100 K fractions, respectively. Our studies demonstrated that fluorescence quenching combined with 2D-COS could be successfully utilized to give insight into the chemical heterogeneity associated with metal-binding sites within the relatively homogeneous HA size fractions.
Styles APA, Harvard, Vancouver, ISO, etc.
7

Huang, Jiaxing. « (Invited) Seeing 2D Sheets with Fluorescence Quenching Microscopy ». ECS Meeting Abstracts MA2020-01, no 10 (1 mai 2020) : 830. http://dx.doi.org/10.1149/ma2020-0110830mtgabs.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
8

Kong, Zhizhi, Matthias Daab, Hitomi Yano, Haiyue Huang, Josef Breu, Takayoshi Sasaki, SonBinh T. Nguyen et Jiaxing Huang. « Visualizing Transparent 2D Sheets by Fluorescence Quenching Microscopy ». Small Methods 4, no 3 (12 février 2020) : 2000036. http://dx.doi.org/10.1002/smtd.202000036.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
9

Xue, Mao-Yun, Ai-Ping Yang, Mei-Hua Ma et Xiao-Hua Li. « The application of two-dimensional fluorescence correlation spectroscopy on the interaction between bovine serum albumin and prulifloxacin ». Spectroscopy 23, no 5-6 (2009) : 257–63. http://dx.doi.org/10.1155/2009/565173.

Texte intégral
Résumé :
The interaction between bovine serum albumin (BSA) and prulifloxacin was investigated by ultraviolet spectrophotometer (UV) and fluorescence spectroscopy in this paper. Two-dimensional (2D) correlation spectroscopy was applied to the analysis of fluorescence spectra. The results of spectroscopic measurements suggested that prulifloxacin (PL) have a strong ability to quench the intrinsic fluorescence of bovine serum albumin through static quenching procedure. Thermodynamic parameter enthalpy changes (ΔH) and entropy changes (ΔS) were calculated. Owing to the spectral resolution enhancement in 2D correlation spectroscopy, the structure change of prulifloxacin can be observed.
Styles APA, Harvard, Vancouver, ISO, etc.
10

Li, Peng, Meng-Yue Guo, Lu-Lu Gao, Xue-Mei Yin, Shuai-Liang Yang, Ran Bu et En-Qing Gao. « Photoresponsivity and antibiotic sensing properties of an entangled tris(pyridinium)-based metal–organic framework ». Dalton Transactions 49, no 22 (2020) : 7488–95. http://dx.doi.org/10.1039/d0dt00397b.

Texte intégral
Résumé :
Novel 2D → 2D entanglement affords close donor–acceptor contacts for electron transfer based photochromism and photomodulable fluorescence of a MOF, which also serves as a regenerable and sensitive luminescent sensor for nitrofuran antibiotics.
Styles APA, Harvard, Vancouver, ISO, etc.
Plus de sources

Thèses sur le sujet "2D-Fluorescence"

1

Loureiro, Cláudia Filipa Reis Galinha. « Monitoring and modelling of membrane bioreactors for wastewater treatment incorporating 2D fluorescence spectroscopy ». Doctoral thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/13108.

Texte intégral
Résumé :
Dissertação para obtenção do Grau de Doutor em Engenharia Química e Bioquímica, Especialidade em Engenharia Bioquímica
Os biorreactores de membranas (MBRs, ‘membrane bioreactors’) para o tratamento de águas residuais combinam o processo de lamas activadas com um passo de filtração para obtenção de um efluente limpo, livre de sólidos. Os MBRs representam uma tecnologia em expansão no tratamento de águas residuais sobretudo devido ao reduzido espaço que requerem e à elevada qualidade do efluente obtido. No entanto, a colmatação das membranas pode reduzir o desempenho do MBR. Por este motivo, no presente trabalho, pretendeu-se estudar a monitorização dos MBRs, com o objectivo de minimizar o número de parâmetros de monitorização necessários para descrever o desempenho do processo e obter uma monitorização em tempo real com recurso mínimo a técnicas laboratoriais demoradas. Para este fim, estudou-se a aplicabilidade da fluorescência bidimensional em meios biológicos complexos, tais como as lamas activadas utilizadas para o tratamento de águas residuais. A fluorescência bidimensional mostrou ser uma técnica abrangente, capaz de recolher informação relevante sobre o estado do sistema em tempo real. Devido à complexidade da informação contida nos espectros de fluorescência, usaram-se técnicas de estatística multivariada, tais como análise de componentes principais e projecção de estruturas latentes (PLS, ‘projection to latent structures’), para extrair a informação dos espectros e correlacioná-la com parâmetros de operação e de desempenho do MBR. O uso de modelos estatísticos permitiu a previsão de parâmetros chave para o desempenho do MBR usando somente dados de processo impostos ou facilmente adquiríveis em tempo real. Adicionalmente, a modelação estatística foi combinada com um modelo mecanístico, numa estrutura híbrida, de forma a melhorar a previsão mecanística. Este estudo demonstrou ser possível usar modelos PLS para incorporar dados de fluorescência obtidos em tempo real, de modo a melhorar a previsão mecanística sem requerer análises laboratoriais adicionais.
Styles APA, Harvard, Vancouver, ISO, etc.
2

Widom, Julia. « Local Conformations and Excited State Dynamics of Porphyrins and Nucleic Acids by 2-Dimensional Fluorescence Spectroscopy ». Thesis, University of Oregon, 2014. http://hdl.handle.net/1794/17885.

Texte intégral
Résumé :
Biological systems present many challenges to researchers attempting to study them using spectroscopy. Low specificity, low sensitivity, and broad and overlapping lineshapes limit the amount of information that can be obtained in experiments. Two-dimensional fluorescence spectroscopy (2D FS) is a highly sensitive and information-rich spectroscopic technique that was developed to study the conformations and excited state dynamics of systems exhibiting exciton coupling. In this dissertation, I describe a variety of extensions of 2D FS that further increase its utility for the study of biological systems. I describe experiments on a dimer of zinc tetraphenylporphyrin embedded in a membrane, in which the signals from two conformational subpopulations were separated in order to study the thermodynamics of their interconversion. I present proof-of-principle experiments on nucleic acids that utilize fluorescence resonance energy transfer to separate signals from different subpopulations. I also describe experiments in which 2D FS was performed using ultraviolet excitation to determine the conformation of a dinucleotide of a fluorescent analogue of the nucleic acid base adenine. I discuss experiments on porphyrin dimers in which 2D FS was used as a probe of excited state dynamics. Finally, I present model calculations for a proposed variation of 2D FS in which entangled photons would be used as the excitation source. These calculations suggest that this approach has the potential to yield significantly narrower spectral lineshapes than conventional 2D FS. These experiments and calculations yield new insight into the systems investigated and establish a `toolbox' of variations of 2D FS that can be used to gain as much information as possible from experiments on challenging systems such as protein-DNA complexes. This dissertation contains previously published and unpublished co-authored material.
Styles APA, Harvard, Vancouver, ISO, etc.
3

Assawajaruwan, Supasuda [Verfasser], et Bernd [Akademischer Betreuer] Hitzmann. « Development of an on-line process monitoring for yeast cultivations via 2D-fluorescence spectroscopy / Supasuda Assawajaruwan ; Betreuer : Bernd Hitzmann ». Hohenheim : Kommunikations-, Informations- und Medienzentrum der Universität Hohenheim, 2019. http://d-nb.info/1177881446/34.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
4

Ranzan, Lucas. « Estudo da viabilidade do uso de espectroscopia por fluorescência 2D para quantificar teor de enxofre em óleo diesel ». reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/116717.

Texte intégral
Résumé :
A dispersão de óxidos sulfurados no meio ambiente, proveniente da queima de combustíveis fosseis, contribui significativamente para a poluição atmosférica. A presença de compostos sulfurados em combustíveis também apresenta desvantagens práticas na operação das unidades, corroendo tubulações e unidades de armazenamento, além de causar danos aos motores. De acordo com a Resolução nº 50, de 23 de dezembro de 2013 da ANP, todo o diesel metropolitano vendido no Brasil deve conter no máximo 10 ppm de enxofre, forçando uma adaptação dos processos produtivos, que, por sua vez, forçam as industrias a investirem em sistemas de controle e consequentemente metodologias de análise on-line de correntes de processo. As análises atuais certificadoras de enxofre em diesel requerem preparação amostral, equipamentos de alto custo e possuem alto tempo morto associado. Surge a necessidade de estudo de uma metodologia capaz de ser aplicada em analisadores de linha para predição de enxofre em tempo real. Com este intuito, este trabalho visa estudar a viabilidade do uso de espectroscopia por fluorescência 2D para predição de enxofre em óleo diesel. A espectroscopia por fluorescência é uma técnica rápida, que dispensa preparação de amostra e possui alta sensibilidade para compostos naturalmente fluorescentes. Um estudo envolvendo quatro componentes sulfurados presentes em diesel foi realizado por meio de metodologias não supervisionadas - PCA, e supervisionadas - PSCM. Não foi possível segmentar as quatro soluções-padrão por meio de gráfico de escores da PCA, mas com PSCM foi factível o ajuste de modelos multilineares para predição de enxofre nas soluções-padrão, com coeficientes de determinação superiores a 0,97. Ainda, é possível definir a região de fluorescência mais significativa para cada um dos padrões, constatando que todos possuem regiões de emissão de fluorescência similares. Com relação à amostras de diesel, foram analisados dois grupos distintos de óleo diesel, (i) diesel HDT com média de 100 ppm de enxofre, e (ii) diesel S10 com média de 6,5 ppm de enxofre. Avaliando os resultados da PCA, foi possível segmentar os dois conjuntos de diesel utilizando os dados de escores. Por PSCM, foi possível ajustar modelos baseados em pares de fluorescência capazes de predizer satisfatoriamente concentrações de enxofre em amostras de diesel S10. Os modelos ajustados para diesel HDT apresentaram resultados menos significativos. Assim, a viabilidade do uso de espectroscopia por fluorescência 2D para a caracterização de enxofre em correntes de diesel foi confirmada, viabilizando a construção de sensores de processos baseados nesta técnica analítica.
The dispersion of sulfur oxides in the environment from the burning of fossil fuels contributes significantly to air pollution. The presence of sulfur compounds in fuel also presents disadvantages in operation units, corroding pipes and storage units and may even damage the engine. According to ANP Resolution No. 50, dated December 23, 2013 the entire metropolitan diesel sold in Brazil must contain no more than 10 ppm sulfur, forcing an adaptation of production processes. The current analysis to certify sulfur content in diesel requires sample preparation, expensive equipment and have high dead time associated. Therefore, it is important the study of a method capable of being applied to online analyzers for predicting sulfur in real time. This work aims to study the feasibility of using 2D fluorescence spectroscopy for prediction of sulfur in diesel fuel. Fluorescence spectroscopy is a rapid technique that, usually, does not require sample preparation and has high sensitivity to naturally fluorescent compounds. Initially, a study involving four sulfur containing compounds present in diesel was carried out by means of unsupervised methods - PCA and supervised - PSCM. It was not possible to segregate the four standard solutions by score plot from the PCA, but PSCM was able to fit general multilinear models for the prediction of sulfur in standard solutions with coefficients of determination greater than 0.97. It was possible to define the region of most significant fluorescence for each standard, noting that all standards present similar fluorescence emission regions. After, two groups of different diesel, diesel HDT averaging 100 ppm sulfur and diesel S10 averaged 6.5 ppm sulfur were analyzed. Evaluating the results of PCA was possible to segregate both sets of diesel data using score plot. With PSCM was possible to find models based on fluorescence able to satisfactorily predict concentrations of sulfur in diesel samples S10. The models adjusted for diesel HDT showed less significant results. Thus, the feasibility of prediction of sulfur in diesel using 2D fluorescence spectroscopy was confirmed.
Styles APA, Harvard, Vancouver, ISO, etc.
5

Pessoa, Carolina de Marco. « Aperfeiçoamento do algoritmo colônia de formigas para o desenvolvimento de modelos quimiométricos ». reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2015. http://hdl.handle.net/10183/118860.

Texte intégral
Résumé :
O desenvolvimento e aperfeiçoamento de métodos de otimização são pontos de profundo interesse em todas as áreas de pesquisa. Tais técnicas muitas vezes envolvem a aquisição de métodos de controle novos ou melhores, o que está diretamente ligado a duas tarefas importantes: a escolha de formas eficientes de monitoramento do processo e a obtenção de modelos confiáveis para a variável de interesse a partir de dados experimentais. Graças às suas diversas vantagens, os sensores óticos vêm sendo amplamente aplicados na primeira tarefa. Uma vez que é possível a utilização de vários tipos de espectroscopia através deste tipo de sensor, modelos capazes de lidar com dados espectrais estão se tornando cada vez mais atraentes. A segunda tarefa, por sua vez, depende não só de quais preditores são utilizados na construção do modelo, mas também de quantos. Como a qualidade do modelo depende também do número de variáveis selecionadas, é importante desenvolver métodos que identifiquem aqueles que explicam o máximo possível da variabilidade dos dados. O método de otimização Colônia de Formigas (ACO) aparece como uma ferramenta bastante útil na seleção de variáveis, podendo-se encontrar muitas variações desse algoritmo na literatura. O propósito deste trabalho é desenvolver métodos de seleção de variáveis com base no algoritmo ACO, conceitos estatísticos e testes de hipóteses. Para isso, diversos critérios de decisão foram implementados nas etapas do algoritmo referentes à atualização de trilha de feromônios (C1) e à seleção de modelos (C2). A fim de estudar estas modificações, foram realizados dois estudos de caso: o primeiro na área de bioprocessos e o segundo na área de caracterização de alimentos. Ambos os estudos mostraram que, em geral, os modelos com menores erros são obtidos utilizando-se métricas dos componentes do modelo, tal como o tamanho do intervalo de confiança de cada parâmetro e o teste-t de hipóteses. Além disso, a modificação do critério de seleção de modelos parece não interferir significativamente no resultado final do algoritmo. Por último, foi feito um estudo da aplicação dessas versões do ACO no campo de caracterização de combustíveis, mais especificamente diesel, associando-se duas análises espectroscópicas para predição do conteúdo de enxofre. Algumas das versões desenvolvidas mostraram-se superior ao algoritmo ACO utilizado como base para este trabalho, proposto por Ranzan (2014), e todas os versões forneceram melhores resultados na quantificação de enxofre que aqueles obtidos por PCR. Dessa forma, comprova-se a potencialidade de métricas implementadas no algoritmo ACO, associadas à espectroscopia, na seleção de preditores significativos.
The development and improvement of optimization methods are points of deep interest in all areas of research. These techniques are often related to the acquisition of new or better control methods, which are directly attached to two importante tasks: choosing efficient forms of process monitoring and obtaining reliable models for the monitored variable from experimental data. Due to their several advantagens, optical sensors are being widely applied in the first task. Since several types of spectroscopy are possible through this type of sensor, models capable of dealing with spectral data are becoming increasingly attractive. The second task depends not only on which predictors are used in the model, but also on how many. Since the quality of the model depends on the number of selected variables, it is important to develop methods that identify those that explain the greater amount of data variability as possible, without compromising the reliability of the model. The Ant Colony Optimization is an important tool for variable selection, being possible to find a lot of variations of this method in literature. The purpose of this work is to develop a method of variable selection based on the Ant Colony Optimization (ACO) algorithm, statistical concepts and hypothesis testing. For this purpose, several decision criteria for trail update (C1) and model selection (C2) were implemented within the routine. In order to study these modifications, two case study was conducted: one related to bioprocess monitoring and another one envolving the characterization of food products. Both studies showed that, in general, the models with the lowest errors were obtained through the use of model component metrics, such as the length of the confidence interval associated with each parameter and the t hypothesis test. Besides, the modification of the model selection criterion doesn’t seem to affect the algorithm final result. Finally, the aplicattion of these methods in the field of fuels characterization, specifically diesel fuel, was studied, associating two spectroscopical analyses in order to predict the sulfur content. Some of the new developed methods appeared to be better than the ACO algorithm used as basis in this work, proposed by Ranzan (2014), and all methods showed better results than those from the models constructed by PCR. Thus, it is proved the high potencial of using different metrics within ACO algorithm, associated with spectroscopy, in order to select significative predictors.
Styles APA, Harvard, Vancouver, ISO, etc.
6

Emmett, Liam. « Synthesis and characterisation of arene borazine hybrids ». Thesis, University of Bath, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.675733.

Texte intégral
Résumé :
We present the synthesis and characterisation of novel single organic molecules known as phenoxylene borazines and borazatruxenes. Using temperature-dependant and concentration-dependant 1H NMR, we probe the supramolecular aggregation of these molecules in solution. Finally, we synthesise 2D hybrid material comprised of electron delocalised benzene rings and electron localised borazine rings. Using a combination of solid-state 11B and 13C NMR techniques, Raman spectroscopy and XPS, we confirm the presence of benzene and borazine regions in these novel materials.
Styles APA, Harvard, Vancouver, ISO, etc.
7

Milliez, Anne. « UP-CONVERSION IN RARE-EARTH DOPED MICRO-PARTICLES APPLIED TO NEW EMISSIVE 2D DISLAYS ». Doctoral diss., University of Central Florida, 2006. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/3966.

Texte intégral
Résumé :
Up-conversion (UC) in rare-earth co-doped fluorides to convert diode laser light in the near infrared to red, green and blue visible light is applied to make possible high performance emissive displays. The infrared-to-visible UC in the materials we study is a sequential form of non-linear two photon absorption in which a strong absorbing constituent absorbs two low energy photons and transfers this energy to another constituent which emits visible light. Some of the UC emitters' most appealing characteristics for displays are: a wide color gamut with very saturated colors, very high brightness operation without damage to the emitters, long lifetimes and efficiencies comparable to those of existing technologies. Other advantages include simplicity of fabrication, versatility of operating modes, and the potential for greatly reduced display weight and depth. Thanks to recent advances in material science and diode laser technology at the excitation wavelength, UC selected materials can be very efficient visible emitters. However, optimal UC efficiencies strongly depend on chosing proper operating conditions. In this thesis, we studied the conditions required for optimization. We demonstrated that high efficiency UC depends on high pump irradiance, low temperature and low scattering. With this understanding we can predict how to optimally use UC emitters in a wide range of applications. In particular, we showed how our very efficient UC emitters can be applied to make full color displays and very efficient white light sources.
Ph.D.
Other
Optics and Photonics
Optics
Styles APA, Harvard, Vancouver, ISO, etc.
8

Simon, Bertrand. « Application des techniques de déconvolution multinoyaux et de tomographie diffractive optique à l'amélioration de l'imagerie microscoique 2D et 3D ». Mulhouse, 2007. http://www.theses.fr/2007MULH0850.

Texte intégral
Résumé :
Le travail de cette thèse porte sur l'amélioration des techniques de microscopies optiques 2D et 3D. Dans une première partie des travaux, j'ai proposé une technique d'illumination structurée focalisée et des raitements numériques spécifiques permettant un gain de la résolution latérale d'un facteur deux en microscopie confocale de fluorescence. La deuxième partie des travaux a consisté à réaliser un montage expérimental de microscopie tomographique optique diffractive qui permet, après une étape de reconstruction numérique, l'imagerie de spécimen transparents en trois imensions. La caractérisation du montage expérimental a montré que cette technique pennettait d'obtenir une information jusqu'alors inaccessible : la cartographie des indices optiques au sein du spécimen observé. En outre un gain en résolution vis à vis des techniques de microscopie plus classiques a été obtenu
This work concerns the improvement of 2D and 3D optical microscopy techniques. In a first part, I propose a focused structured illumination technique with specific numérical processing allowing an improvement of a factor two of the lateral resolution in confocal fluorescence microscopy. The second part of work consisted in carrying an experimental diffractive optical tomographic microscopy set-up. This one allows, after a stage of numerical processing of the images, to image 3D transparent specimens. The characterization of the experimental set-up showed that this technique allowed to obtain information not accessible before : the cartography of the optical indices within the specimen observed. Moreover a improvement of the resolution with respect to the more traditional techniques of microscopy was obtained
Styles APA, Harvard, Vancouver, ISO, etc.
9

Karlsson, Edlund Patrick. « Methods and models for 2D and 3D image analysis in microscopy, in particular for the study of muscle cells ». Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9201.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
10

Santos, Felipe André dos [UNESP]. « Determinação de manganês e zinco em spots protéicos de plasma de tilápia do Nilo (Oreochromis niloticus) por SR-XRF e GFAAS após separação por 2D-PACE ». Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/95264.

Texte intégral
Résumé :
Made available in DSpace on 2014-06-11T19:27:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-06-15Bitstream added on 2014-06-13T20:36:06Z : No. of bitstreams: 1 santos_fa_me_botfmvz.pdf: 1235942 bytes, checksum: 7b3f579b06b2874c741bde8ef170587e (MD5)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Universidade Estadual Paulista (UNESP)
O presente trabalho teve como objetivo investigar a presença de manganês e zinco em “spots” protéicos de amostras de plasma de tilápia do Nilo (Oreochromis niloticus) obtidos após separação das proteínas por eletroforese em gel de poliacrilamida em segunda dimensão (2D-PAGE) para posterior avaliação qualitativa e quantitativa utilizando fluorescência de raios-X com radiação síncrotron (SR-XRF) e espectrometria de absorção atômica em chama e em forno de grafite (FAAS/GFAAS). As análises dos espectros de fluorescência indicaram a presença de manganês e zinco em quatro e seis “spots” protéicos de plasma, respectivamente. Observou-se que os íons metálicos estão ligados em proteínas com massa molar na faixa de 19 a 70 kDa e com pI na faixa de 4,7 a 6,30. A concentração de manganês e zinco ligados a essas proteínas foi determinada por GFAAS após a mineralização ácida dos spots protéicos, encontrandose concentrações na faixa de 3,40 a 4,20 mg g-1 e 2,30 a 13,90 mg g-1, respectivamente
The aim of the present study was to investigate the presence of manganese and zinc in protein spots in samples of Nile tilapia (Oreochromis niloticus) plasma obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and subsequent qualitative and quantitative determination by synchrotron radiation X-ray fluorescence (SRXRF) and graphite furnace atomic absorption spectrometry (GFAAS). An analysis of the fluorescence spectra indicated the presence of manganese and zinc in four and six plasma protein spots, respectively. It was observed that the metal ions are bound in proteins with molecular weight ranging from 19 to 70 kDa and pI ranging from 4.7 to 6.30. The manganese and zinc concentrations bound to these proteins were determined by GFAAS after acid digestion of protein spots, finding concentrations ranging from 3.40 to 4.20 mg g-1 and 2.30 to 13.90 mg g - 1, respectively
Styles APA, Harvard, Vancouver, ISO, etc.
Plus de sources

Chapitres de livres sur le sujet "2D-Fluorescence"

1

Robotti, Elisa, et Emilio Marengo. « 2D-DIGE and Fluorescence Image Analysis ». Dans Methods in Molecular Biology, 25–39. New York, NY : Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7268-5_3.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
2

Wang, Daifa, Ling Wang, Yubo Fan et Deyu Li. « 2D Shape-based fluorescence molecular tomography through hybrid genetic algorithm based optimization ». Dans IFMBE Proceedings, 1018–21. Berlin, Heidelberg : Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-29305-4_267.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
3

Castelli, Filippo M., Matteo Roffilli, Giacomo Mazzamuto, Irene Costantini, Ludovico Silvestri et Francesco S. Pavone. « Semantic Segmentation of Neuronal Bodies in Fluorescence Microscopy Using a 2D+3D CNN Training Strategy with Sparsely Annotated Data ». Dans Machine Learning, Optimization, and Data Science, 95–99. Cham : Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-64583-0_10.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
4

Ishii, Kunihiko, Takuhiro Otosu et Tahei Tahara. « Lifetime-Weighted FCS and 2D FLCS : Advanced Application of Time-Tagged TCSPC ». Dans Springer Series on Fluorescence, 111–28. Cham : Springer International Publishing, 2014. http://dx.doi.org/10.1007/4243_2014_65.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
5

Rodríguez-Vidal, Francisco. « Application of Excitation-Emission Matrix Fluorescence (EEMF) in the Wastewater Field ». Dans Fluorescence Imaging - Recent Advances and Applications [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.105975.

Texte intégral
Résumé :
Fluorescence is a versatile and useful analytical technique for the analysis of waters, both natural waters (freshwaters and marine waters) and wastewaters (urban wastewaters and industrial effluents). Among the various fluorescence techniques currently available, excitation-emission matrix fluorescence (EEMF) is the most used nowadays since it provides comprehensive information on the dissolved organic matter (DOM) present in water. EEMF spectra can be represented either in the form of a 3D-graph or a 2D-contour map and fluorescence peaks can be studied by the fast and simple peak-picking method (more suitable for routine measurements in water treatment plants, allowing a rapid response in case of potential problems in the sequence of treatment) or using mathematical tools such as PARAFAC (more suitable for research purposes and accurate identification of the fluorophores). The EEMF peaks commonly found in waters are peaks A and C (humic substances), peaks B1, B2, T1, and T2 (protein-like peaks), and peak M (microbial-like peak). EEMF was first applied to the characterization of natural waters, but in recent years, more attention is being paid to the wastewater field. Urban wastewaters have been mostly studied, whereas there are fewer studies focused on industrial effluents. This chapter provides a brief review of these EEFM applications.
Styles APA, Harvard, Vancouver, ISO, etc.
6

Najumnissa D. « Comparison and Analysis of Dental Imaging Techniques ». Dans Computational Techniques for Dental Image Analysis, 179–94. IGI Global, 2019. http://dx.doi.org/10.4018/978-1-5225-6243-6.ch008.

Texte intégral
Résumé :
Fluoride dental decay is the second most common disease around the world. Detection methods for early disease are very crude. Precise oral diagnosis and treatment are very strongly connected to the quality of dental imaging techniques which advances the diagnostic procedure. To study the external appearance of the teeth arches, 2D images are used. CBCT images were used to locate the bone at dental implant sites. Fiberoptic transillumination, fluorescence imaging detects caries. For qualitative and quantitative analysis of dental applications, laser-induced breakdown spectroscopy (LIBS) is used. Electron caries monitor (ECM), fiberoptic transillumination (FOTI), digital fiberoptic transillumination (DIFOTI), quantitative light-induced fluorescence (QLF) are also some of the detection methods used. Hence, in this chapter, the methodologies are analyzed and compared for easy use of the dentist.
Styles APA, Harvard, Vancouver, ISO, etc.
7

Takeda, S., G. Picard, M. Yamaki et K. Nagayama. « Preparation and significance of fluorescence labeled recombinant apoferritin for in situ characterization of 2D protein arrays on mercury ». Dans Advanced Materials '93, 509–12. Elsevier, 1994. http://dx.doi.org/10.1016/b978-1-4832-8380-7.50123-1.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.

Actes de conférences sur le sujet "2D-Fluorescence"

1

Simionovici, A. « 2D imaging by X-ray fluorescence microtomography ». Dans Sixth international conference on x-ray microscopy (XRM99). AIP, 2000. http://dx.doi.org/10.1063/1.1291207.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
2

Qu, Jun L., Hanben Niu, Ji Li, Xiaomei Kuo et Junlan Zhou. « Time-domain 2D fluorescence lifetime microscopic measurement ». Dans Medical Imaging 2000, sous la direction de James T. Dobbins III et John M. Boone. SPIE, 2000. http://dx.doi.org/10.1117/12.384525.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
3

Li, Haijun, Zhen Qiu, Xiyu Duan, Kenn R. Oldham, Katsuo Kurabayashi et Thomas D. Wang. « 2D resonant microscanner for dual axes confocal fluorescence endomicroscope ». Dans 2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2014. http://dx.doi.org/10.1109/memsys.2014.6765763.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
4

Katz, Alvin, Howard E. Savage, Yuanlong Yang, Fanan Zeng, Jayson Rome, Steven A. McCormick, Rubina S. Cocker, Yizhong Yu et Robert R. Alfano. « Ultraviolent and blue 2D fluorescence mapping of gynecological tissues ». Dans BiOS 2000 The International Symposium on Biomedical Optics, sous la direction de Robert R. Alfano. SPIE, 2000. http://dx.doi.org/10.1117/12.382734.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
5

Liu, Qian, Yu Chen, Britton Chance et Qingming Luo. « Low-frequency phased-array 2D fluorescence localization in breast cancer detection ». Dans Third International Conference on Photonics and Imaging in Biology and Medicine, sous la direction de Qingming Luo, Valery V. Tuchin, Min Gu et Lihong V. Wang. SPIE, 2003. http://dx.doi.org/10.1117/12.546147.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
6

Yacomotti, A. M., L. C. Estrada, O. E. Martinez, M. Brunstein, L. Le-Gratiet, S. Bouchoule, A. Talneau, I. Sagnes et A. Levenson. « Fluorescence Microscopy on a 2D Photonic Crystal Transparent in the Visible ». Dans Conference on Lasers and Electro-Optics. Washington, D.C. : OSA, 2010. http://dx.doi.org/10.1364/cleo.2010.ctun6.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
7

Wang, Daifa, et Jing Bai. « Triangle Mesh Based 2D Fluorescence Molecular Tomography with Spatially Smoothed Linear Scheme ». Dans Asia Communications and Photonics Conference and Exhibition. Washington, D.C. : OSA, 2009. http://dx.doi.org/10.1364/acp.2009.tucc4.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
8

Kaskelyte, Dalia, Arunas Ciburys, Saulius Bagdonas, Giedre Streckyte, Ricardas Rotomskis et Roaldas Gadonas. « Fiber-optics based laser system for 2D fluorescence detection and optical biopsy ». Dans Clinical and Biomedical Spectroscopy. SPIE, 2009. http://dx.doi.org/10.1117/12.831582.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
9

Larson, Jeffrey M. « 2D and 3D deconvolution of confocal fluorescence images by maximum likelihood estimation ». Dans International Symposium on Biomedical Optics, sous la direction de Jose-Angel Conchello, Carol J. Cogswell et Tony Wilson. SPIE, 2002. http://dx.doi.org/10.1117/12.467835.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
10

Qi, Xin, Zhuo Wang et Xuantao Su. « Detection of microparticles in flow by 2D light scattering and fluorescence imaging ». Dans Twelfth International Conference on Information Optics and Photonics, sous la direction de Yue Yang. SPIE, 2021. http://dx.doi.org/10.1117/12.2606539.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.

Rapports d'organisations sur le sujet "2D-Fluorescence"

1

Creighton, Dan. X-ray diffraction, x-ray fluorescence, pyrolysis, source rock analysis, 2D SEM images from cuttings for wells : Malguk 1, Merak 1, Pipeline State 1, West Kavik 1, and Wolfbutton 25-6-9. Alaska Division of Geological & Geophysical Surveys, juillet 2020. http://dx.doi.org/10.14509/30533.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
2

Eyal, Yoram, et Sheila McCormick. Molecular Mechanisms of Pollen-Pistil Interactions in Interspecific Crossing Barriers in the Tomato Family. United States Department of Agriculture, mai 2000. http://dx.doi.org/10.32747/2000.7573076.bard.

Texte intégral
Résumé :
During the evolutionary process of speciation in plants, naturally occurring barriers to reproduction have developed that affect the transfer of genes within and between related species. These barriers can occur at several different levels beginning with pollination-barriers and ending with hybrid-breakdown. The interaction between pollen and pistils presents one of the major barriers to intra- and inter-specific crosses and is the focus of this research project. Our long-term goal in this research proposal was defined to resolve questions on recognition and communication during pollen-pistil interactions in the extended tomato family. In this context, this work was initiated and planned to study the potential involvement of tomato pollen-specific receptor-like kinases (RLK's) in the interaction between pollen and pistils. By special permission from BARD the objectives of this research were extended to include studies on pollen-pistil interactions and pollination barriers in horticultural crops with an emphasis on citrus. Functional characterization of 2 pollen-specific RLK's from tomato was carried out. The data shows that both encode functional kinases that were active as recombinant proteins. One of the kinases was shown to accumulate mainly after pollen germination and to be phosphorylated in-vitro in pollen membranes as well as in-vivo. The presence of style extract resulted in dephosphorylation of the RLK, although no species specificity was observed. This data implies a role for at least one RLK in pollination events following pollen germination. However, a transgenic plant analysis of the RLK's comprising overexpression, dominant-negative and anti-sense constructs failed to provide answers on their role in pollination. While genetic effects on some of the plants were observed in both the Israeli and American labs, no clear functional answers were obtained. An alternative approach to addressing function was pursued by screening for an artificial ligand for the receptor domain using a peptide phage display library. An enriched peptide sequence was obtained and will be used to design a peptide-ligand to be tested for its effect o pollen germination and tube growth. Self-incompatibility (SI) in citrus was studied on 3 varieties of pummelo. SI was observed using fluorescence microscopy in each of the 3 varieties and compatibility relations between varieties was determined. An initial screen for an S-RNase SI mechanism yielded only a cDNA homologous to the group of S-like RNases, suggesting that SI results from an as yet unknown mechanism. 2D gel electrophoresis was applied to compare pollen and style profiles of different compatibility groups. A "polymorphic" protein band from style extracts was observed, isolated and micro-sequenced. Degenerate primers designed based on the peptide sequence date will be used to isolate the relevant genes i order to study their potential involvement in SI. A study on SI in the apple cultivar Top red was initiated. SI was found, as previously shown, to be complete thus requiring a compatible pollinator variety. A new S-RNase allele was discovered fro Top red styles and was found to be highly homologous to pear S-RNases, suggesting that evolution of these genes pre-dated speciation into apples and pears but not to other Rosaceae species. The new allele provides molecular-genetic tools to determine potential pollinators for the variety Top red as well as a tool to break-down SI in this important variety.
Styles APA, Harvard, Vancouver, ISO, etc.
Vous pouvez également être intéressé par les bibliographies sur le sujet « 2D-Fluorescence » pour d’autres types de sources :
Articles de revues Thèses
Nous offrons des réductions sur tous les plans premium pour les auteurs dont les œuvres sont incluses dans des sélections littéraires thématiques. Contactez-nous pour obtenir un code promo unique!

Vers la bibliographie