Literatura académica sobre el tema "Wzb phosphatase"
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Artículos de revistas sobre el tema "Wzb phosphatase"
Reid, Anne N. y Chris Whitfield. "Functional Analysis of Conserved Gene Products Involved in Assembly of Escherichia coli Capsules and Exopolysaccharides: Evidence for Molecular Recognition between Wza and Wzc for Colanic Acid Biosynthesis". Journal of Bacteriology 187, n.º 15 (1 de agosto de 2005): 5470–81. http://dx.doi.org/10.1128/jb.187.15.5470-5481.2005.
Texto completoVincent, Carole, Patricia Doublet, Christophe Grangeasse, Elisabeth Vaganay, Alain J. Cozzone y Bertrand Duclos. "Cells of Escherichia coli Contain a Protein-Tyrosine Kinase, Wzc, and a Phosphotyrosine-Protein Phosphatase, Wzb". Journal of Bacteriology 181, n.º 11 (1 de junio de 1999): 3472–77. http://dx.doi.org/10.1128/jb.181.11.3472-3477.1999.
Texto completoNakar, David y David L. Gutnick. "Involvement of a Protein Tyrosine Kinase in Production of the Polymeric Bioemulsifier Emulsan from the Oil-Degrading Strain Acinetobacter lwoffii RAG-1". Journal of Bacteriology 185, n.º 3 (1 de febrero de 2003): 1001–9. http://dx.doi.org/10.1128/jb.185.3.1001-1009.2003.
Texto completoRosche, Thomas M., Ben Smith y James D. Oliver. "Evidence for an Intermediate Colony Morphology of Vibrio vulnificus". Applied and Environmental Microbiology 72, n.º 6 (junio de 2006): 4356–59. http://dx.doi.org/10.1128/aem.02937-05.
Texto completoLaPointe, Gisele, Daniele Atlan y Christophe Gilbert. "Characterization and site-directed mutagenesis of Wzb, an O-phosphatase from Lactobacillus rhamnosus". BMC Biochemistry 9, n.º 1 (2008): 10. http://dx.doi.org/10.1186/1471-2091-9-10.
Texto completoTiwari, Monalisa, Shruti Panwar, Akansha Kothidar y Vishvanath Tiwari. "Rational targeting of Wzb phosphatase and Wzc kinase interaction inhibits extracellular polysaccharides synthesis and biofilm formation in Acinetobacter baumannii". Carbohydrate Research 492 (junio de 2020): 108025. http://dx.doi.org/10.1016/j.carres.2020.108025.
Texto completoGovarthanan, Muthusamy, Jung-Hee Park, Loganathan Praburaman, Young-Joo Yi, Min Cho, Hyun Myung, Shanmugam Gnanendra, Seralathan Kamala-Kannan y Byung-Taek Oh. "Relative Expression of Low Molecular Weight Protein, Tyrosine Phosphatase (Wzb Gene) of Herbaspirillum sp. GW103 Toward Arsenic Stress and Molecular Modeling". Current Microbiology 71, n.º 3 (6 de junio de 2015): 311–16. http://dx.doi.org/10.1007/s00284-015-0850-6.
Texto completoKlumpp, Susanne, Jan Hermesmeier, Dagmar Selke, Ralf Baumeister, Roland Kellner y Josef Krieglstein. "Protein Histidine Phosphatase: A Novel Enzyme with Potency for Neuronal Signaling". Journal of Cerebral Blood Flow & Metabolism 22, n.º 12 (diciembre de 2002): 1420–24. http://dx.doi.org/10.1097/01.wcb.0000045041.03034.99.
Texto completoPettis, Gregg S. y Aheli S. Mukerji. "Structure, Function, and Regulation of the Essential Virulence Factor Capsular Polysaccharide of Vibrio vulnificus". International Journal of Molecular Sciences 21, n.º 9 (5 de mayo de 2020): 3259. http://dx.doi.org/10.3390/ijms21093259.
Texto completoCefalo, Angela D., Jeffery R. Broadbent y Dennis L. Welker. "The Streptococcus thermophilus protein Wzh functions as a phosphotyrosine phosphatase". Canadian Journal of Microbiology 59, n.º 6 (junio de 2013): 391–98. http://dx.doi.org/10.1139/cjm-2013-0094.
Texto completoTesis sobre el tema "Wzb phosphatase"
Iodice, Domenico. "Construction and characterization of probiotic Lactobacillus rhamnosus GG deletion mutant for extracellular polysaccharide (EPS) biosynthesis regulator". Doctoral thesis, Università di Siena, 2018. http://hdl.handle.net/11365/1037713.
Texto completoHuang, Yu-Chie y 黃愈志. "Structural Studies of Human RNase 7 and Klebsiella pneumoniae Phosphatase Wzb". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/51053237025758269998.
Texto completo國立臺灣大學
生化科學研究所
97
Chapter 1 Ribonucleases are found widely within living organisms and are thought to play important roles in RNA metabolism, angiogenesis, neurotoxicity, antitumor and antimicrobial activity. In human ribonucleases, antimicrobial activity was found in RNase 3, RNase 5 and RNase 7. The mechanisms of antimicrobial activities remain unclear although they possess similar properties, e.g., high isoelectric point and net positive charge. It is therefore interesting to investigate whether the positively charged residues or epitopes are responsible for the bactericidal activity. Here, we report on the role of cationic residues of human RNase 7 (hRNase 7) in its antimicrobial activity. We found that hRNase 7 is stable in the range of pH 3.5~9.5 and its structure is thermally and chemically stable with a Tm value of 66.4 ℃ and a Cm value of 3.27 M denatured by guanine hydrochloride. NMR structure of hRNase 7 shows that the 22 positively charged residues (18 Lys, 4 Arg) are distributed into three clusters on the surface. Residues in the first cluster including Lys1, Lys3, Lys111 and Lys112, are quite flexible and located near the N-terminus. On the contrary, the other two clusters, one with residues Lys32/Lys35 and the other with Lys96/Arg97/Lys100, are all located on secondary structure regions and are quite rigid. Mutagenesis studies confirmed that residues in the flexible cluster are critical for the bactericidal activity rather than the catalytic residues, such as His15, Lys38, His123, and residues in the other two positively charged clusters. Together, we suggest that hRNase 7 binds to bacterial membrane primarily by using the flexible residues, Lys1, Lys3, Lys111, and Lys112, in the first positively charged cluster and then renders the membrane permeable. Chapter 2 The low molecular weight protein tyrosine phosphatases (LMW-PTPs) in bacteria are ubiquitous regulators of tyrosine phosphorylation. Despite their abundance, the spectrum of functions of LMW-PTPs in prokaryote has yet to be elucidated. Several studies have demonstrated a role of LMW-PTPs in CPS/EPS synthesis and export, as well as in stress resistance. The target protein, LMW-PTPs Wzb, studied in this thesis is from the K. pneumoniae NTUH2044 strain clinically isolated at National Taiwan University Hospital (NTUH). It contains the conserved signature motif of CX5RS known as the P-loop or PTPloop, and an unique Asp residue situated on the DPY-loop of the LMW-PTPs. Our previous study has found that catalytic activity on Wzb_C9S mutant is dramatically reduced or even not seen at all. To ascertain the importance of the conversed residue Cys9 in the catalytic mechanism of Wzb, we expressed and purified unlabeled and isotopically labeled Wzb and Wzb_C9S mutant for structural study. Due to the lack of several cross peaks in NMR spectral of Wzb, we could not determine its structure. In comparison, Wzb_C9S gave much better NMR quality and its 3D NMR solution structure containing a canonical topology of LMW-PTPs with a central four-stranded parallel beta-sheet and five alpha-helices has been solved. Superimposition of 2D HSQC spectra of Wzb and Wzb_C9S reveals that the unobserved cross peaks and cross peaks exhibiting shift changes in Wzb are mostly located in the P-loop and its nearby regions. Backbone dynamics study showed that the residues in the P-loop are moderately rigid. In addition, both Tm and Cm values in Wzb_C9S are higher than those in Wzb. Taken together, we conclude there is a conformational change, especially in the P-loop, between Wzb and Wzb_C9S, with the P-loop in Wzb_C9S apparently more rigid. Due to the change of the rigidity in the P-loop, the Ser9 in C9S mutant is unable to act as a nucleophile to attack the phosphate group, as Cys9 does in Wzb.
Hong-TsunChang y 張宏存. "iPhos: a web tool to streamline the alkaline phosphatase-assisted LC-MS phosphoproteome investigation". Thesis, 2015. http://ndltd.ncl.edu.tw/handle/7n3cf5.
Texto completo國立成功大學
電機工程學系
103
iPhos is a web based software toolkit to facilitate and streamline the work-flow of the alkaline phosphatase-assisted phosphoproteome characterization. Comprehensive characterization of the phosphoproteome in living cells is critical in signal transduction research. But the low abundance of phosphopeptides among the total proteome in cells remains a challenge in mass spectrometry-based proteomic analysis. To overcome the obstacles, an alternative analytic strategy to confidently identify phosphorylated peptides by using the alkaline phosphatase (AP) treatment in combination with high-resolution mass spectrometry was provided by Liao’s group. While the process is applicable, the key integration along the pipeline was mostly done by tedious manual work. In order to be effective and convenient, iPhos not only has a user friendly web interface but also assists the experiment work-flow in data analysis. We further demonstrated the utility of the iPhos toolkit on the data of human metastatic lung cancer cells (CL1-5). In the comparison study of the control group of CL1-5 cell lysates and the treatment group of dasatinib-treated CL1-5 cell lysates, we demonstrated the applicability of the iPhos toolkit and reported the experimental results based on the iPhos-facilitated phosphoproteome investigation. Additionally, we also compared the strategy with pure DDAbased LC-MS/MS phosphoproteome investigation. The results of iPhos-facilitated targeted LC-MS/MS analysis convey more thorough and confident phosphopeptide identification than the results of pure DDA-based analysis. The iPhos software toolkit and sample tutorial data are available online at http://cosbi3.ee.ncku.edu.tw/iPhos/.
Chao, Ti-chun y 趙悌均. "PSTPIP2, Proline-Serine-Threonine Phosphatase Interacting Protein 2, a Host Membrane-Deforming Protein, is Critical for Membranous Web Formation in Hepatitis C Virus Replication". Thesis, 2011. http://ndltd.ncl.edu.tw/handle/06552578558701366866.
Texto completoIslam, Salim Timo. "Structural and Functional Characterization of O-Antigen Translocation and Polymerization in Pseudomonas aeruginosa PAO1". Thesis, 2013. http://hdl.handle.net/10214/7239.
Texto completoBookmarks within the document have been provided for ease of access to a particular section in the body of the thesis. Each entry in the Table of Contents, List of Tables, and List of Figures has been "linked" to its respective position and as such can be clicked for direct access to the entry. Similarly, each in-text Figure or Table reference has been "linked" to its respective figure/table for direct access to the entry.
1.) Canadian Institutes of Health Research (CIHR) Frederick Banting and Charles Best Canada Graduate Scholarship doctoral award, 2.) CIHR Michael Smith Foreign Study Award, 3.) Cystic Fibrosis Canada (CFC) doctoral studentship, 4.) University of Guelph Dean's Tri-Council Scholarship, 5.) Ontario Graduate Scholarship in Science and Technology, 6.) Operating grants to Dr. Joseph S. Lam from CIHR (MOP-14687) and CFC
Libros sobre el tema "Wzb phosphatase"
Kirchman, David L. Degradation of organic matter. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198789406.003.0007.
Texto completoCapítulos de libros sobre el tema "Wzb phosphatase"
Sharma, Sunita. "Biosynthesis of the Immunomodulatory Molecule Capsular Polysaccharide A from Bacteroides fragilis". En Biosynthesis [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96937.
Texto completoActas de conferencias sobre el tema "Wzb phosphatase"
Nishi, Toshihiro, Kazunori Naganuma, Yoshihisa Sakai, Masao Asobe y Shoichi Sudo. "Amplification characteristics of femtosecond optical pulses in short, Er3+-doped phosphate fibers". En OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1993. http://dx.doi.org/10.1364/oam.1993.wb.1.
Texto completoXia, Da-Hai, Chengcheng Pan, Shi-Zhe Song, Wei-Xian Jin, Wen-Bin Hu y Bao-Min Fan. "Covalent surface modification of 2024 aluminum alloy surface by self-assembly dodecyl phosphate film towards corrosion protection". En 1st Corrosion and Materials Degradation Web Conference. Basel, Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/cmdwc2021-10040.
Texto completoDAGDAG, Omar, Zaki Safi, Lei Guo, Hamid Erramli y Mustapha El Gouri. "Assessment of the anticorrosive efficiency of zinc phosphate (ZP) and epoxy resin (DGEBA-DAA) based composite (DGEBA-DAA-ZP) for electrolytic cadmium pre-treated 15CDV6 steel in 3wt% NaCl medium: experimental and computational studies". En 1st Corrosion and Materials Degradation Web Conference. Basel, Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/cmdwc2021-09984.
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