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Bibliografías temáticas / Ultra-Fast detection

Literatura académica sobre el tema "Ultra-Fast detection"

Autor: Grafiati

Publicado: 22 de junio de 2024

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Artículos de revistas sobre el tema "Ultra-Fast detection":

1

Schmid, Maximilian, Erdogan Celik, Sepideh Mazloomzadeh, Stefan Aschauer y Heike Soltau. "Optimized Ultra-Fast Low Dose Electron Detection". Microscopy and Microanalysis 28, S1 (22 de julio de 2022): 2198–99. http://dx.doi.org/10.1017/s1431927622008480.

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Wang, Jian, Yuesong Zhang, Fei Zhang, Yazhou Li, Lingcong Nie y Jiale Zhao. "MegaDetectNet: A Fast Object Detection Framework for Ultra-High-Resolution Images". Electronics 12, n.º 18 (5 de septiembre de 2023): 3737. http://dx.doi.org/10.3390/electronics12183737.

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Addressing the challenge of efficiently detecting objects in ultra-high-resolution images during object detection tasks, this paper proposes a novel method called MegaDetectNet, which leverages foreground image for large-scale resolution image object detection. MegaDetectNet utilizes a foreground extraction network to generate a foreground image that highlights target regions, thus avoiding the computationally intensive process of dividing the image into multiple sub-images for detection, and significantly improving the efficiency of object detection. The foreground extraction network in MegaDetectNet is built upon the YOLOv5 model with modifications: the large object detection head and classifier are removed, and the PConv convolution is introduced to reconstruct the C3 module, thereby accelerating the convolution process and enhancing foreground extraction efficiency. Furthermore, a Res2Rep convolutional structure is developed to enlarge the receptive field and improve the accuracy of foreground extraction. Finally, a foreground image construction method is proposed, fusing and stitching foreground target regions into a unified foreground image. This approach replaces multiple divided sub-images with a single foreground image for detection, reducing overhead time. The proposed MegaDetectNet method’s effectiveness for detecting objects in ultra-high-resolution images is validated using the publicly available DOTA dataset. Experimental results demonstrate that MegaDetectNet achieves an average time reduction of 83.8% compared to the sub-image division method among various commonly used object detectors, with only a marginal 8.7% decrease in mAP (mean Average Precision). This validates the practicality and efficacy of the MegaDetectNet method for object detection in ultra-high-resolution images.

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Brembilla, G., F. Giganti, H. Sidhu, M. Imbriaco, H. U. Ahmed, C. Moore, M. Emberton y S. Punwani. "Ultra-fast biparametric MRI for prostate cancer detection". European Urology 79 (junio de 2021): S1262. http://dx.doi.org/10.1016/s0302-2838(21)01274-4.

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Shin, M. K., G. I. Moon y Y. E. Koo. "Development of ultra-fast detection method for GMOs". New Biotechnology 44 (octubre de 2018): S78—S79. http://dx.doi.org/10.1016/j.nbt.2018.05.904.

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Maceiczyk, Richard M., David Hess, Flora W. Y. Chiu, Stavros Stavrakis y Andrew J. deMello. "Differential detection photothermal spectroscopy: towards ultra-fast and sensitive label-free detection in picoliter & femtoliter droplets". Lab Chip 17, n.º 21 (2017): 3654–63. http://dx.doi.org/10.1039/c7lc00946a.

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6

Liu, LinYue, XiaoPing Ouyang, ZhongBing Zhang, JianFu Zhang, XianPeng Zhang, YunHong Zhong y Wei Wang. "Polycrystalline chemical-vapor-deposited diamond for fast and ultra-fast neutron detection". Science China Technological Sciences 55, n.º 9 (29 de junio de 2012): 2640–45. http://dx.doi.org/10.1007/s11431-012-4948-9.

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Waldeck, Stephan, Rania Helal, Ibraheem Al-Dhamari, Sandra Schmidt, Christian von Falck, René Chapot, Marc Brockmann y Daniel Overhoff. "New ultra-fast algorithm for cochlear implant misalignment detection". European Journal of Radiology 151 (junio de 2022): 110283. http://dx.doi.org/10.1016/j.ejrad.2022.110283.

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Dey, Sayan, Sumita Santra, Anupam Midya, Prasanta Kumar Guha y Samit Kumar Ray. "Synthesis of CuxNi(1−x)O coral-like nanostructures and their application in the design of a reusable toxic heavy metal ion sensor based on an adsorption-mediated electrochemical technique". Environmental Science: Nano 4, n.º 1 (2017): 191–202. http://dx.doi.org/10.1039/c6en00285d.

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9

Qi, Yun, Yan Zhao, Haihong Bao, Wei Jin y Hoi Lut Ho. "Nanofiber enhanced stimulated Raman spectroscopy for ultra-fast, ultra-sensitive hydrogen detection with ultra-wide dynamic range". Optica 6, n.º 5 (30 de abril de 2019): 570. http://dx.doi.org/10.1364/optica.6.000570.

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10

Seel, Matthias, Eberhard Wildermuth y Wolfgang Zinth. "A multichannel detection system for application in ultra-fast spectroscopy". Measurement Science and Technology 8, n.º 4 (1 de abril de 1997): 449–52. http://dx.doi.org/10.1088/0957-0233/8/4/014.

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Tesis sobre el tema "Ultra-Fast detection":

1

Stein, Oliver [Verfasser] y Eckhard [Akademischer Betreuer] Elsen. "Ultra Fast Beam Loss Mechanisms at the LHC and their Detection for Use in Machine Protection / Oliver Stein ; Betreuer: Eckhard Elsen". Hamburg : Staats- und Universitätsbibliothek Hamburg, 2018. http://d-nb.info/1171427344/34.

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Le, Marchand Tanguy. "Protein Dynamics by Solid-State NMR with Ultra-Fast Magic-Angle Spinning : from Microcrystals to Amyloid Fibrils and Membrane Proteins". Thesis, Lyon, 2018. http://www.theses.fr/2018LYSEN023/document.

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La Résonance Magnétique Nucléaire (RMN) à l’état solide avec rotation à l’angle magique (MAS) est une technique de choix pour l’étude de la structure et de la dynamique de molécules biologiques peu ou non solubles. Un grand nombre d’approches ont été développées pour la reconstitution de structures tridimensionelles à partir de mesures précises de proximités internucléaires, ainsi que pour la détection de mouvements moléculaires avec une résolution atomique sur des échelles de temps couvrant plusieurs ordres de grandeur. Malgré d’impressionnants progrès, les études par RMN MAS sont cependant loin d’être réalisées en routine. Les déterminations structurelles et de dynamique sont souvent démontrées sur des préparations microcristallines modèles, mais sont encore rares pour des systèmes plus complexes tels que les fibrilles amyloïdes non cristallines ou les protéines trans-membranaires insérées dans des bi- couches lipidiques. Mon travail a pour objectif d’étendre les possibilités de la RMN MAS pour l’étude de systèmes biomoléculaires complexes dans différents états d’agrégation. Pour cela, j’ai exploité les possibilités uniques offertes par les hauts champs magnétiques (fréquence de Larmor du 1H 700, 800 et 1000 MHz) combinés avec les sondes MAS de dernières générations capables d’atteindre des vitesses de rotations supérieures à 60 kHz. Ces conditions expérimentales per- mettent d’augmenter la sensibilité de la RMN MAS à l’aide de la détection 1H à haute résolution et d’enrichir la palette de paramètres RMN rapporteurs de la dynamique des protéines. La première partie de cette thèse décrit le développement de nouvelles stratégies pour l’attribution des résonances du squelette de protéines, pour l’élucidation de structures, et pour l’étude de la dynamique du squelette peptidique et des chaînes latérales. Les méthodes présentées réduisent significative- ment les besoins en termes de temps expérimental, de quantités d’échantillon et de marquage isotopique, et permettent d’analyser par RMN des systèmes de plus hauts poids moléculaire. La seconde partie décrit l’application de la RMN MAS avec détection en 1H pour l’évaluation du rôle de la dynamique des protéines dans des processus tels que la formation de fibrilles amyloïdes et le fonctionnement de protéines membranaires. Une première application est l’étude de la tendance de la β-2 microglobuline humaine à former des fibrilles amyloïdes. Une comparaison de la dynamique du squelette peptidique de la protéine sauvage et du mutant D76N dans leur forme cristalline, ainsi que la détermination de propriétés structurales de la forme fibrillaire m’ont permis d’identifier la présence de repliements pathologiques et de formuler des hypothèses sur le mécanisme de formation des fibrilles. Finalement, la dynamique locale et globale de protéines membranaires dans des bicouches lipidiques a été étudiée. En particulier, le mécanisme d’action d’un transporteur d’alkanes, AlkL, de P. putida a été examiné dans un environnement lipidique. La détermination de paramètres pour la dynamique rapide (ps-ns) et lente (μs-ms) du squelette peptidique de la protéine en présence ou en absence de substrat met en évidence des acheminements possibles pour le transfert de molécules vers la membrane et jette les bases pour une meilleure compréhension du processus
Solid-state NMR with magic angle spinning (MAS) has emerged as a powerful technique for investigating structure and dynamics of insoluble or poorly soluble biomolecules. A number of approaches has been designed for reconstructing molecular structures from the accurate measurement of internuclear proximities, and for probing motions at atomic resolution over timescales spanning several orders of magnitude. Despite this impressive progress, however, MAS NMR studies are still far from routine. Complete determinations, which are often demonstrated on model microcrystalline preparations, are still rare when it comes to more complex systems such as non-crystalline amyloid fibrils or transmembrane proteins in lipid bilayers. My work aimed at extending the possibilities of MAS NMR for applications on complex biomolecular systems in different aggregation states. For this, I exploited the unique possibilities provided by high magnetic fields (700, 800 and 1000 MHz 1H Larmor frequency) in combination with the newest MAS probes capable of spinning rates exceeding 60 kHz. These experimental conditions al- low to boost the sensitivity of MAS NMR through 1H detection at high resolution and to enrich the palette of probes for protein dynamics. The first part of the thesis reports on my contribution to the development of new strategies for backbone resonance assignment, for structure elucidation, and for investigation of backbone and side-chain dynamics. These methodologies significantly reduce the requirements in terms of experimental time, sample quantities and isotopic labeling, and enlarge the molecular size of systems amenable to NMR analysis. The second part describes the application of 1H detected MAS NMR to evaluate the role of protein dynamics in problems such as amyloid fibril formation and membrane protein function. I first addressed the amyloid fibril formation propensity of human beta-2 microglobulin, the light chain of the major histocompatibility complex I. I performed comparative studies of backbone dynamics of the wild type protein as well as a D76N mutant in crystals, and determined some of the structural features of the fibrillar form. This allowed to identify the presence of pathological folding intermediates and to formulate hypotheses on the mechanism of fibrils formation. Finally, I studied the local and global dynamics of membrane proteins in lipid bilayers. In particular, I investigated the mechanism of action of the alkane trans- porter AlkL from P. putida in lipid bilayers. The measurement of parameters for fast (ps-ns) and slow (μs-ms) backbone dynamics of the protein in presence or in absence of a substrate highlights possible routes for molecular uptake and lays the basis for a more detailed mechanistic understanding of the process

3

Hanoun, Christelle. "Development of time-stretch terahertz waveform recorders for high repetition rate accelerator-based light sources". Electronic Thesis or Diss., Université de Lille (2022-....), 2023. https://pepite-depot.univ-lille.fr/ToutIDP/EDSMRE/2023/2023ULILR072.pdf.

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Le travail de Thèse concerne le développement de systèmes permettant l'enregistrement de signaux électriques, en single-shot, avec des bandes passantes allant jusqu'à plusieurs térahertz (THz). Ce travail est motivé par des besoins importants dans le développement et la recherche sur les sources de lumière basées sur des accélérateurs (les centres de rayonnement synchrotron et les lasers à électrons libres). Ce travail est également motivé par les besoins récents de spectroscopie THz avec des fréquences d'acquisition élevées. Cette thèse se focalise sur le développement de systèmes de mesure où les ondes THz à analyser sont sondées au moyen d'un laser femtoseconde. Plus spécifiquement, le travail porte sur le développement de la technique dite du photonic time-stretch, et vise à résoudre plusieurs problèmes ouverts. Jusque récemment, les techniques de time-stretch souffraient d'une limite fondamentale sur leurs durées d'enregistrement et/ou leurs résolutions temporelles à des valeurs incompatibles avec de nombreuses applications dans le domaine des accélérateurs. Dans une première partie, le travail a consisté à tenter de résoudre ce problème en développant, au laboratoire PhLAM, un système d'enregistrement THz monocoup associant la technique du photonic time-stretch et la technique dite du Diversity Electro-Optic Sampling (DEOS). Le système d'enregistrement, basé sur un laser de sonde à 1030 nm a ensuite été testé lors de deux séries d'expériences sur les sources THz intense basées sur l'accélérateur de ELBE, à Dresde. La première expérience s'est focalisée sur la source CDR (Coherent Diffraction Radiation) de ELBE - une source THz émettant des impulsions “single cycle”. Le succès de cette expérience nous a ensuite mené à réaliser des mesures du rayonnement émis par le laser à électrons libres (FEL) Térahertz de FELBE. Ceci nous a permis de démontrer la possibilité d'enregistrer de signaux THz à une fréquence - record - de 13 MHz. De plus, d'un point de vue plus fondamental, l'étude expérimentale du démarrage du laser à électrons libres a permis - pour la première fois - de visualiser de façon directe le démarrage d'un laser impulsionnel, en enregistrant complètement les impulsions émises (c'est-à-dire leur amplitude et leur porteuse). Finalement, cette thèse s'est focalisée sur un problème ouvert différent, concernant le coût - extrêmement élevé - des systèmes de mesures THz de type time-stretch. En effet, ces systèmes de mesures requièrent des oscilloscopes avec des bandes passantes élevées (généralement au-delà de 10 ou 20 GHz). À partir d'une étude comparative détaillée, et de mesures effectuées à SOLEIL, nous avons démontré la supériorité d'une stratégie basée sur des lasers de sonde à 1550 nm, au lieu des lasers (essentiellement à 1030 nm) usuellement employés dans la littérature. En permettant l'utilisation d'oscilloscopes avec des bandes passantes relativement faibles (de l'ordre du GHz), ceci nous a permis de réduire les coûts de façon importante, permettant d'envisager une popularisation beaucoup plus importante de ces méthodes de time-stretch dans le domaine des accélérateurs et en spectroscopie
Terahertz (THz) science lacks of non-destructive waveform recorders for single-shot measurements of ultrafast signals. Such recording systems are particularly needed in accelerator-based light sources, such as synchrotron radiation facilities and Free-Electron Lasers (FEL). Single-shot operation is required for monitoring the emission of THz FELs, as well as the emission by other novel coherent THz sources. Moreover, single-shot recording systems are also required for monitoring shot-to-shot fluctuations of relativistic electron bunch properties, either for fundamental research, and in routine accelerator operation. This Thesis focuses on the development of THz recorders, using laser probes, that can operate at high repetition rates, typically in the Megahertz range. A main point of the strategy consists of using the so-called photonic time-stretch technique, for imprinting the THz waveform under interest onto a chirped laser pulse, and then to stretch it in time, so that it can be recorded by an oscilloscope. Two main designs are presented. In a first time we present a time-stretch-based recorder that is able to record waveforms with unprecedented duration and/or time resolution, by associating the time-stretch technique, with the recently developed Diversity Electro-Optic Sampling method (DEOS). We then present the first tests of this method on the THz Coherent Diffraction Radiation beamline of the ELBE facility (at the Helmoltz Zentrum Dresden Rossendorf). Using this system, we then present the first measurements of the pulses emitted by a THz Free-Electron Laser, the FELBE FEL, operating at 13 MHz repetition rate. This represents the first complete recording of pulses (amplitude and carrier) not only in a Free-Electron Laser, but also in a mode locked laser in general. Finally, we address the open problem of costs in THz time stretch systems, which are dominated by the required high bandwidth oscilloscopes (several hundreds of k€ as of 2023). We show that, when using the 1550 nm wavelength for the laser probe, special designs of THz time-strech digitizers can lead to much lower costs. We finally show a proof-of concept test of this method at the THz AILES beamline of the SOLEIL facility

4

Marchetti, Alessandro. "Sviluppi metodologici per la cristallizzazione e l’analisi strutturale di proteine tramite Risonanza Magnetica Nucleare allo stato solido". Doctoral thesis, Scuola Normale Superiore, 2012. http://hdl.handle.net/11384/85789.

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High-resolution solid-state NMR (ssNMR) has recently emerged as a powerful characterization technique for systems that cannot be investigated by solution NMR or X-ray crystallographic methods, and represents a subtle complementary technique for any atomic-scaled study. This is particularly true in structural biology. There exist nowadays well established protocols for sample preparation, resonance assignment and collection of structural restraints, that have paved the way to the first three-dimensional structure determinations at atomic resolution of biomolecules in the solid state, from microcrystalline samples to fibrils and membrane-associated systems. Despite rapid uptake in the field of structural biology, however, these methods for structure determination are far from being routine, and several important problems remain however to be solved before ssNMR is applied to the study of challenging solid protein assemblies. Many methodological developments are still expected in this fast evolving field. Most of the model systems used up-to-date for method development in biological solid-state NMR, are relatively small globular proteins, in the range of 50 to 80 residues (approximately 5.5 to 9.5 kDa). In order to extend the capabilities of ssNMR to larger substrates, the objectives of this thesis are twofold: a) to establish a new, large and more complex model system, and b) to develop new, sophisticated NMR experiments in order to improve the sensitivity and the resolution of the currently existing schemes for resonance assignment, which is one of the main barrier to progress to structural investigation in solid proteins. The N-terminal domain of the subunit of E. coli DNA polymerase III (186 : 186 residues, 18 kDa) was selected as a target. This domain represents the catalytic core of the E. coli replisome, the large molecular machine that replicated DNA in bacteria. In a first part, preparation conditions for solid-state NMR are obtained, notably in combination with automated screening processes for high-throughput protein crystallography, and almost complete resonance assignment is performed by the application of established experiments based on high-power rf irradiations and slow magic-angle spinning (MAS) at high magnetic fields. In a second part, we explore the use of MAS at so-called ultra-fast spinning rates (60 kHz). We show that this makes possible the use of “totally low power” experiments. This yields an extraordinary increase in resolution and sensitivity, enabling the acquisition of selective cross- polarization (CP) transfers, through-bond correlations and 1 H-detected correlations. In particular, we demonstrate that narrow 1 H NMR line widths can be obtained for fully protonated protein samples in the solid state under ultra-fast magic-angle spinning for medium-size microcrystalline and non-crystalline proteins, without any need for dilution against a deuterated background. This provides extensive, robust and expeditious assignments of the backbone 1 H, 15 N, 13 Cα and 13 CO resonances of proteins in different aggregation states, without the need of deuteration. The final part of this thesis concerns the study of thermotropic liquid crystals (LC or LX) phases of a de Vries smectogen, the (S)-hexyl-lactate derivative abbreviated as 9HL, selectively deuterated in a phenyl moiety of the aromatic core. de Vries mesophases show a substantially constant layer spacing in the transition between smectic C and smectic A mesophases and are for this reason of great interest for the development of new ferroelectric (FLC) and antiferroelectric (AFLC) electrooptic devices. Our work is the first attempt to apply NMR to characterize the nature of the de Vries transition, discriminating among possible models. It is also one of the first examples in the scientific literature of application of high magnetic field (above 16 T) for the analysis of LX phases.

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Barbet-Massin, Emeline. "Structure of bio-macromolecular complexes by solid-state Nuclear Magnetic Resonance". Thesis, Lyon, École normale supérieure, 2013. http://www.theses.fr/2013ENSL0811.

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La RMN du solide a récemment émergé en tant que technique très puissante en biologie structurale, permettant de caractériser au niveau atomique des systèmes qui ne peuvent être étudiés par d’autres méthodes. Des protocoles spécifiques à la RMN du solide sont à présent bien établis pour la préparation des échantillons, l’attribution des spectres et l’acquisition de contraintes structurales. Ensemble, ces protocoles ont ouvert la voie aux premières déterminations de structures tridimensionnelles de molécules biologiques à l’état solide avec une résolution atomique, et ce non seulement pour des échantillons protéiques microcristallins, mais également pour des systèmes plus complexes tels que des fibrilles ou des protéines membranaires.La détermination structurale de tels systèmes est cependant encore loin d’être une routine, et des avancées de plus large ampleur sont attendues grâce à des développements aux niveaux méthodologique et matériel. Pour cette raison, une majeure partie du travail présenté dans cette thèse a été consacrée au développement d’expériences à la fois nouvelles et sophistiquées pour améliorer la sensibilité et la résolution des méthodes déjà existantes pour attribuer les spectres et élargir les possibilités offertes par la RMN du solide en vue d’étudier la structure de systèmes protéiques plus larges. Ces développements reposent notamment sur l’utilisation de champs magnétiques très intenses et sur la rotation des échantillons à l’angle magique dans la gamme des très hautes vitesses angulaires. Nous montrons que dans ce cadre, il est possible de concevoir des expériences utilisant uniquement des champs radiofréquences à faible puissance ainsi que d’utiliser des transferts sélectifs, l’acquisition de corrélations à travers les liaisons chimiques et la détection proton.En particulier, nous montrons que des expériences de corrélation homonucléaire reposant sur des transferts scalaires deviennent une alternative compétitive aux expériences basées sur des transferts dipolaires. Deux nouvelles séquences d’impulsion permettant de détecter des corrélations 13C-13C à travers les liaisons chimiques avec une excellente résolution sont présentées; couplées à des transferts 15N-13C, elles permettent l’attribution des résonances de la chaîne principale des protéines avec une grande sensibilité.De plus, nous démontrons qu’il est possible d’obtenir des raies très fines pour les résonances de protons dans des protéines complètement protonées à l’état solide grâce à la rotation à l’angle magique à ultra-haute vitesse, sans avoir recours à la deutération. Dans ce contexte, nous avons développé de nouvelles stratégies permettant d’attribuer rapidement et de façon fiable les résonances des spins 1H, 15N, 13CO, 13CA et 13CB dans différentes classes de protéines, ainsi que pour mesurer des contraintes structurales à partir des distances entre protons. L’approche proposée repose sur la haute sensibilité des protons et accélère donc considérablement les processus d’attribution et de détermination structurale des protéines à l’état solide, comme illustré sur la protéine beta-2-microglobuline.Enfin, nous avons appliqué cette nouvelle approche pour réaliser l’attribution et l’étude structurale et fonctionnelle de trois catégories de complexes protéiques: les fibrilles amyloidogènes formées par beta-2-microglobuline, les nucléocapsides du virus de la rougeole, et les nucléocapsides d’Acinetobacter phage205. Nous avons également utilisé la technique de Polarisation Nucléaire Dynamique pour obtenir des informations sur l’ARN des nucléocapsides du virus de la rougeole.Nous considérons que les résultats présentés dans cette thèse représentent une avancée substantielle dans le domaine de la RMN du solide appliquée à la biologie structurale. Grâce aux progrès actuels dans ce domaine, l’impact de la RMN biomoléculaire à l’état solide promet d’augmenter dans les prochaines années
Solid-state NMR has recently emerged as a key technique in modern structural biology, by providing information at atomic level for the characterization of a wide range of systems that cannot be investigated by other atomic-scale methods. There are now well established protocols for sample preparation, resonance assignment and collection of structural restraints, that have paved the way to the first three-dimensional structure determinations at atomic resolution of biomolecules in the solid state, from microcrystalline samples to fibrils and membrane-associated systems. These determinations are however still far from being routine, and larger breakthroughs are expected with further methodological and hardware developments. Accordingly, most of the work presented in this thesis consists of the development of new, sophisticated NMR experiments to improve the sensitivity and resolution of the currently existing schemes for resonance assignment and to extend the capabilities of solid-state NMR in terms of structural investigation of proteins for the analysis of large substrates. These developments notably rely on the use of very high magnetic fields and ultra-fast magic-angle spinning (MAS). We show the great potential of this particular regime, which enables the use of low-power experiments and the acquisition of selective cross-polarization transfers, through-bond correlations and 1H-detected correlations.In particular, we show that homonuclear correlation experiments based on through-bond transfers become competitive alternatives to dipolar transfer schemes. Two new pulse sequences that detect sensitive and resolved 13C-13C through-bond correlations are introduced, which coupled to 15N-13C dipolar transfer steps provide sensitive routes for protein backbone resonance assignment.Furthermore, we demonstrate that narrow 1H NMR line widths can be obtained for fully protonated proteins in the solid state under ultra-fast MAS, even without perdeuteration. In this context, we have developed new strategies for extensive, robust and expeditious assignments of the 1H, 15N, 13CO, 13CA and 13CB resonances of proteins in different aggregation states, and new schemes for the measurements of site-specific 1H-1H distance restraints. This approach relying on the very high sensitivity of 1H spins remarkably accelerates the processes of assignment and structure determination of proteins in the solid state, as shown by the assignment and de novo structure determination of native beta-2-microglobulin. Finally, we apply this new approach to perform resonance assignment and to study structural and dynamic features of three complex protein aggregates: amyloid fibrils formed by native and D76N beta-2-microglobulin, Acinetobacter phage 205 nucleocapsids and measles virus (MeV) nucleocapsids. We also used Dynamic Nuclear Polarization to obtain the first information about RNA in MeV nucleocapsids.We believe that the results presented in this thesis represent a substantial step forward for solid-state NMR in structural biology. With all the current advances in the field, the impact of biomolecular solid-state NMR is likely to increase in the next years

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Vignola, Gianpiero. "Realizzazione di un sistema automatico di test per rivelatori al silicio e studio preliminare di una matrice 2x2 con UFSD". Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amslaurea.unibo.it/16927/.

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Obiettivo di questo studio è quello di realizzare un programma per la caratterizzazione degli innovativi rivelatori UFSD (Ultra Fast Silicon Detectors) a disposizione della sede di Bologna dell'INFN, per poi passare all'analisi preliminare di un prototipo di matrice 2 x 2 di rivelatori dello stesso tipo. I risultati ottenuti sono stati confrontati con le caratteristiche fornite dai costruttori. Per quanto riguarda la matrice, si sono effettuate delle scansioni utilizzando una fonte laser. Si è quindi valutata la risposta del dispositivo concentrandosi sulle differenze in ampiezza dei segnali ottenuti. Nel Capitolo 1 si illustrano brevemente i principi fisici alla base del funzionamento dei rivelatori al silicio, soffermandosi principalmente su quelli caratterizzati dalla presenza di uno strato di guadagno interno. Nel Capitolo 2 viene descritto l'apparato di misura utilizzato, i rivelatori analizzati e i programmi LabVIEW che ho realizzato per effettuare le misure. Questi sono stati progettati nell'ottica di rendere rapide ed efficienti le future caratterizzazioni di UFSD o altri dispositivi a semiconduttore. I risultati ottenuti sono mostrati e analizzati nel Capitolo 3.

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Soldat, Jan [Verfasser] y Peter [Akademischer Betreuer] Fischer. "Characterization, Operation and Wafer-level Testing of an ultra-fast 4k Pixel Readout ASIC for the DSSC X-ray Detector at the European XFEL / Jan Soldat ; Betreuer: Peter Fischer". Heidelberg : Universitätsbibliothek Heidelberg, 2018. http://d-nb.info/1177691531/34.

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Strazzi, Sofia. "Study of first thin LGAD prototypes for the ALICE 3 timing layers". Master's thesis, Alma Mater Studiorum - Università di Bologna, 2021. http://amslaurea.unibo.it/24382/.

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The work here presented concerns the characterization and the performance study of very thin Low-Gain Avalanche Detector (LGAD) prototypes; the goal is to evaluate if such a sensor is suitable for the Time-Of-Flight (TOF) system of the ALICE 3 experiment, a next generation heavy-ion experiment (LHC Run 5). A total of 18 sensors with a thickness of 25 μm and 35 μm were characterized; both single channel and matrices, with different inter-pad design and doping profile were compared to two 50 μm-prototypes. Preliminary tests with a laser setup allowed to evaluate the light-sensitive areas in terms of efficiency, uniformity of the response and edge effects. Finally, timing performances were analyzed. Promising results were found for the 25 μm-thick sensors, which showed a time resolution better than 16 ps for a gain 20 and reaching nearly 13 ps for a gain 30.

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Godin, David Andrew. "Development of an UFLC/MS/MS method for the comparative analysis of oxytocin and artesunate-amodiaquine for validation of field detection systems". Thesis, 2016. https://hdl.handle.net/2144/19190.

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Spurious, falsely-labeled, falsified or counterfeit (SFFC) pharmaceuticals are a health concern that claims hundreds of thousands of lives annually1, a violation of intellectual property rights which cost legitimate companies billions2, and a low-risk high yield revenue stream for organized crime2. While ports of entry and border control points are the primary access control points for SFFC3,4, advances in field portable detection and equipment offers an increasingly effective method for the assessment of pharmaceuticals at regional centers and points of distribution. This is particularly important for less developed countries (LDC) who do not maintain satellite or regional testing facilities. As part of a proposed protocol to assess field portable detection equipment, an ultrafast liquid chromatography, tandem mass spectrometry (UFLC-MS/MS) method for the quantification of liquid formulation Oxytocin was developed. The six minute method was found to have a within run %bias of +/-16%, a linear dynamic range of 150-1000 nanograms/milliliter (ng/ml), and an accuracy within acceptability criteria for all tested concentrations. The effectiveness of three identified transition ions, 723.1, 86.2 and 70.1 Daltons, for the analysis of oxytocin by mass spectrometry was assessed across several figures of merit to include signal to noise ratio, %CV, calibration sensitivity, and analytical sensitivity. The 723.1 ion fragment was recommended for quantification, while the 70.1 dalton ion was recommended as a qualifier ion, although 86.2 also performed within acceptability criteria. A method for the UFLC-MS/MS assessment of degradation products for oxytocin was proposed for specificity testing. Degradation of oxytocin by exposure to highly acidic, basic, and thermal conditions for one hour was attempted. Formation of degraded products was not observed. Additionally, existing High Performance Liquid Chromatography (HPLC) methods for the simultaneous assessment of Artesunate and Amodiaquine HCl were modified to assess compatibility with UFLC. No method assessed produced sufficient quality signal to continue with method development.

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Walsh, Erin. "Detection of drugs of abuse within electronic cigarette liquids using gas chromatography-mass spectrometry and ultra fast liquid chromatography tandem mass spectrometry". Thesis, 2015. https://hdl.handle.net/2144/13971.

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As electronic cigarettes become more prevalent in society, their use as a delivery mechanism for drugs of abuse has increased. Electronic cigarette liquids present a complex matrix due to the lack of regulation, and therefore standardization, in their manufacturing. Due to the lack of published data, development of new analytical methods to accommodate this complexity was deemed necessary. Gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-tandem mass spectrometry (LC/MS/MS) methods were developed to identify the flavorants of the electronic cigarette liquids as well as identify and quantify nicotine and common drugs of abuse used with these devices. Seven drugs of abuse were investigated: methamphetamine, heroin, cocaine, fentanyl, JWH-081, JWH-018, and AM-2201. Electronic cigarette liquids from five manufacturers were sampled. From each manufacturer five "flavors" of liquids were chosen. Each liquid "flavor" was tested at the manufactures reported nicotine concentrations of 0 mg/mL, 12 mg/mL, and 24 mg/mL to give a total of 75 electronic cigarette liquid samples. Liquid-liquid extraction was performed on all samples prior to analysis by GC/MS and LC/MS/MS. Analysis was performed in replicates of five to identify the electronic cigarette liquid components as well as quantify nicotine and the four analytes of interest. For any electronic cigarette liquid labeled as containing 0 mg/mL of nicotine in which nicotine was identified, the sample was analyzed by GC/MS to quantify the nicotine level. These concentrations were compared to the naturally occurring levels of nicotine found in certain food products. Identification of the flavorants of the electronic cigarette liquids as well as the quantitation of nicotine and the four commonly abused drugs was accomplished using GC/MS and LC/MS/MS. Samples of e-liquids labeled by the manufacturer as containing 0 mg/mL of nicotine may contain detectable and quantifiable levels of nicotine. Quantitation of drugs of abuse may be affected by matrix components and was found to be dependent on both the specific e-liquid being used with the electronic cigarette as well as the analyte being investigated.

Capítulos de libros sobre el tema "Ultra-Fast detection":

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Qin, Zequn, Huanyu Wang y Xi Li. "Ultra Fast Structure-Aware Deep Lane Detection". En Computer Vision – ECCV 2020, 276–91. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-58586-0_17.

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Schöffler, M. S., L. Ph H. Schmidt, S. Eckart, R. Dörner, A. Czasch, O. Jagutzki, T. Jahnke et al. "Ultra-fast Dynamics in Quantum Systems Revealed by Particle Motion as Clock". En Molecular Beams in Physics and Chemistry, 353–73. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-63963-1_17.

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AbstractTo explore ultra-fast dynamics in quantum systems one needs detection schemes which allow time measurements in the attosecond regime. During the recent decades, the pump & probe two-pulse laser technique has provided milestone results on ultra-fast dynamics with femto- and attosecond time resolution. Today this technique is applied in many laboratories around the globe, since complete pump & probe systems are commercially available. It is, however, less known or even forgotten that ultra-fast dynamics has been investigated several decades earlier even with zeptosecond resolution in ion-atom collision processes. A few of such historic experiments, are presented here, where the particle motion (due to its very fast velocity) was used as chronometer to determine ultra-short time delays in quantum reaction processes. Finally, an outlook is given when in near future relativistic heavy ion beams are available which allow a novel kind of “pump & probe” experiments on molecular systems with a few zeptosecond resolution. However, such experiments are only feasible if the complete many-particle fragmentation process can be imaged with high momentum resolution by state-of-the-art multi-particle coincidence technique.

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Jünger, Daniel, Christian Hundt, Jorge González-Domínguez y Bertil Schmidt. "Ultra-Fast Detection of Higher-Order Epistatic Interactions on GPUs". En Euro-Par 2016: Parallel Processing Workshops, 421–32. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-58943-5_34.

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Li, Wenkai y Andy Song. "UFO RPN: A Region Proposal Network for Ultra Fast Object Detection". En Lecture Notes in Computer Science, 619–31. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-97546-3_50.

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Retianza, Darian Verdy, Jeroen van Duivenbode, Henk Huisman y Maurice Roes. "A Robust and Ultra-Fast Short Circuit Detection in Half-Bridge Using Stray Voltage Capture: Applied in Electromagnetic Suspension". En Lecture Notes in Mobility, 244–53. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-65871-7_19.

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Patel, Madhavi y Vishal Patel. "High Throughput Screening Techniques in Anticancer Drug Discovery and Development from Botanicals". En Alternative Remedies and Natural Products for Cancer Therapy: An Integrative Approach, 97–121. BENTHAM SCIENCE PUBLISHERS, 2023. http://dx.doi.org/10.2174/9789815124699123010008.

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Botanicals obtained from nature are the major source of therapeutic medicines for humans. Utilization of botanicals for a variety of diseases is not mysterious, and a rapid increase in such therapeutic substances is observed due to their efficacy and long-term safety. Cancer, as a leading cause of disease worldwide, piques the interest of researchers seeking novel anticancer agents. Till date, the major share of cancer medicines is occupied by natural products, and the drug discovery process is rapidly going on. However, traditional anticancer drug discovery is time-consuming and herculean. High-throughput screening (HTS) is a tool to make natural product library screening easy and fast. The advancement of extraction, isolation, and structure characterization of Phyto actives obtained from botanicals, provides a large number of compounds for testing via HTS. High-density well plates, a liquid handling platform, automation, and robotics allow the screening of 100,000 compounds per day. Anticancer screening of botanicals by HTS can be performed on various cancer cell lines along with molecular targets, enzyme or protein interaction assays, or the capacity of the extract to induce apoptosis. After the replication of identified extracts, further assays are performed for more precise results. HTS screening and computational methods provide speed and a high degree of sensitivity for anticancer agents and compress the time required for drug discovery. Advances in HTS technology, such as ultra-HTS and the use of 3-D cultures, will speed up the process of discovering anticancer drugs from botanicals. Advanced detection techniques for HTS assays, as well as instrumental techniques for identified lead compound separation, help to ensure that the results are neat.

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Kenidra, Billel y Mohamed Benmohammed. "An Ultra-Fast Method for Clustering of Big Genomic Data". En Research Anthology on Bioinformatics, Genomics, and Computational Biology, 175–91. IGI Global, 2023. http://dx.doi.org/10.4018/979-8-3693-3026-5.ch008.

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The clustering process is used to identify cancer subtypes based on gene expression and DNA methylation datasets, since cancer subtype information is critically important for understanding tumor heterogeneity, detecting previously unknown clusters of biological samples, which are usually associated with unknown types of cancer will, in turn, gives way to prescribe more effective treatments for patients. This is because cancer has varying subtypes which often respond disparately to the same treatment. While the DNA methylation database is extremely large-scale datasets, running time still remains a major challenge. Actually, traditional clustering algorithms are too slow to handle biological high-dimensional datasets, they usually require large amounts of computational time. The proposed clustering algorithm extraordinarily overcomes all others in terms of running time, it is able to rapidly identify a set of biologically relevant clusters in large-scale DNA methylation datasets, its superiority over the others has been demonstrated regarding its relative speed.

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Huang, M. H. A., S. Ahmad, P. Barrillon, S. Brandt, C. Budtz-Jørgensen, A. J. Castro-Tirado, S. H. Chang et al. "THE CALIBRATION AND SIMULATION OF THE GRB TRIGGER DETECTOR OF THE ULTRA FAST FLASH OBSERVATORY". En Gamma-ray Bursts: 15 Years of GRB Afterglows, 531–36. EDP Sciences, 2020. http://dx.doi.org/10.1051/978-2-7598-1002-4-088.

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Huang, M. H. A., S. Ahmad, P. Barrillon, S. Brandt, C. Budtz-Jørgensen, A. J. Castro-Tirado, S. H. Chang et al. "THE CALIBRATION AND SIMULATION OF THE GRB TRIGGER DETECTOR OF THE ULTRA FAST FLASH OBSERVATORY". En Gamma-ray Bursts: 15 Years of GRB Afterglows, 531–36. EDP Sciences, 2020. http://dx.doi.org/10.1051/978-2-7598-1002-4.c088.

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Actas de conferencias sobre el tema "Ultra-Fast detection":

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Alshawi, Tariq. "Ultra-Fast Saliency Detection Using Qr Factorization". En 2019 53rd Asilomar Conference on Signals, Systems, and Computers. IEEE, 2019. http://dx.doi.org/10.1109/ieeeconf44664.2019.9048740.

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Melentiev, Pavel N., Rinat O. Esenaliev, Lina V. Son, Denis S. Kudryavtsev, Igor E. Kasheverov, Victor I. Tsetlin y Victor I. Balykin. "Ultra-fast, ultra-sensitive single molecule detection and real-time imaging". En Single Molecule Spectroscopy and Superresolution Imaging XV, editado por Ingo Gregor, Rainer Erdmann y Felix Koberling. SPIE, 2022. http://dx.doi.org/10.1117/12.2617863.

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Kolakowski, Jerzy. "Detection of UWB pulses with ultra-fast comparators". En 2014 20th International Conference on Microwaves, Radar, and Wireless Communications (MIKON). IEEE, 2014. http://dx.doi.org/10.1109/mikon.2014.6899992.

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Lembrikov, B. I. y Y. Ben-Ezra. "Ultra fast detection based on SiGe thin layers". En 2010 12th International Conference on Transparent Optical Networks (ICTON). IEEE, 2010. http://dx.doi.org/10.1109/icton.2010.5549024.

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Quenum, Jerome, Kehan Wang y Avideh Zakhor. "Fast, Accurate Barcode Detection in Ultra High-Resolution Images". En 2021 IEEE International Conference on Image Processing (ICIP). IEEE, 2021. http://dx.doi.org/10.1109/icip42928.2021.9506134.

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Paulillo, B., S. Pirotta, S. Guilet, P. Crozat, J. M. Manceau, N. Zerounian, A. Degiron et al. "Sub-wavelength THz resonators for ultra-fast THz detection". En SPIE OPTO, editado por Manijeh Razeghi. SPIE, 2017. http://dx.doi.org/10.1117/12.2251405.

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Cojocari, O., N. Sobornytskyy, C. Weickhmann, R. Jakoby, A. Semenov, H. Hubers, R. Muller y A. Hoehl. "Quasi optical Schottky diode detectors for fast ultra-wideband detection". En 2016 IEEE International Conference on Microwave and Millimeter Wave Technology (ICMMT). IEEE, 2016. http://dx.doi.org/10.1109/icmmt.2016.7761674.

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Lashgari, Mahmoud y S. Mohammad Shahrtash. "Ultra-Fast Detection and Location of Busbar Faults Employing IMG". En 2020 14th International Conference on Protection and Automation of Power Systems (IPAPS). IEEE, 2019. http://dx.doi.org/10.1109/ipaps49326.2019.9069380.

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Sobornytskyy, N., A. Lisauskas, C. Weickhmann, R. Jakobi, A. Semenov, H. Hubers, R. Muller, A. Hoehl y O. Cojocari. "Quasi optical Schottky diode detectors for fast ultra-wideband detection". En 2013 38th International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz 2013). IEEE, 2013. http://dx.doi.org/10.1109/irmmw-thz.2013.6665618.

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Vermeulen, O., A. Manzanera y L. Lacassagne. "Ultra Fast Grey Scale Face Detection Using Vector SIMD Programming". En 2007 Third International IEEE Conference on Signal-Image Technologies and Internet-Based System SITIS. IEEE, 2007. http://dx.doi.org/10.1109/sitis.2007.142.

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Informes sobre el tema "Ultra-Fast detection":

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Tringe, J., N. Ileri, S. Letant, P. Stroeve, M. Shirk, S. Zaidi, R. Balhorn y C. Siders. Ultra-fast Laser Synthesis of Nanopore Arrays in Silicon for Bio-molecule Separation and Detection. Office of Scientific and Technical Information (OSTI), febrero de 2008. http://dx.doi.org/10.2172/926003.

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Jordan, Tyler Alexander. Development of an ultra-fast BaF₂-based detector. Office of Scientific and Technical Information (OSTI), abril de 2020. http://dx.doi.org/10.2172/1615640.

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Jordan, Tyler. Development of an ultra-fast BaF2-based detector. Office of Scientific and Technical Information (OSTI), noviembre de 2020. http://dx.doi.org/10.2172/1726114.

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