Tesis sobre el tema "Tumour metastasis"
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Clark, S. R. "The investigation of tumour metastasis". Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370241.
Texto completoKnight, C. Rosamund L. "Transglutaminase activity, tumour growth and metastasis". Thesis, Nottingham Trent University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278115.
Texto completoHand, D. "The importance of transglutaminase in tumour growth and metastasis". Thesis, Nottingham Trent University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382575.
Texto completoChen, Dongsheng. "Transcriptional regulation of the p9Ka gene in metastatic and non-metastatic rat mammary epithelial cells". Thesis, University of Liverpool, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266488.
Texto completoLi, Chao. "Investigation of the influence of tissue factor on tumour metastasis". Thesis, University of Hull, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.441584.
Texto completoRoyston, Daniel John. "The role of LYVE-1 in tumour metastasis and inflammation". Thesis, University of Oxford, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558401.
Texto completoTexler, Michael Lutz. "Aetiology of tumour cell movement during laparoscopic surgery : patterns of movement and influencing factors". Title page, table of contents and abstract only, 1999. http://web4.library.adelaide.edu.au/theses/09MD/09mdt355.pdf.
Texto completoJack, A. S. "A study of the factors which affect the growth of tumour cells at distant sites". Thesis, University of Glasgow, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381493.
Texto completoLundberg, Owe. "Laparoscopy and tumour growth : a clinical and experimental study". Doctoral thesis, Umeå : Univ, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-227.
Texto completoBalathasan, Lukxmi. "Characterising the role of circulating immune cells in brain metastasis". Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:e7620d30-7e4a-468b-b819-db4cf27eaef6.
Texto completoAbu-Izneid, Tareq y n/a. "The Synthesis and Evaluation of Functionalised Carbohydrates as Probes of Tumour Metastasis". Griffith University. Institute for Glycomics, 2005. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20061019.111424.
Texto completoNixdorf, Sheri Clinical School Prince of Wales Hospital Faculty of Medicine UNSW. "Studies of tumour and metastasis suppressor genes in colorectal and bladder cancer". Awarded by:University of New South Wales. Clinical School - Prince of Wales Hospital, 2009. http://handle.unsw.edu.au/1959.4/44541.
Texto completoRmali, Khaled Ali Ramadan. "Tumour-associated angiogenesis in the development and metastasis of human colorectal cancer". Thesis, Cardiff University, 2006. http://orca.cf.ac.uk/54099/.
Texto completoAlbon, Jacob George. "Developing and using atomic force microscopy to investigate mechanisms of tumour metastasis". Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/7815/.
Texto completoAbu-Izneid, Tareq. "The Synthesis and Evaluation of Functionalised Carbohydrates as Probes of Tumour Metastasis". Thesis, Griffith University, 2005. http://hdl.handle.net/10072/367269.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
Institute for Glycomics
Full Text
Homer, Jarrod James. "Studies on angiogenesis in head and neck squamous cell carcinoma". Thesis, University of Hull, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342866.
Texto completoLeber, Thomas Matthias. "Regulation of MMP-9 in human ovarian cancer". Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298185.
Texto completoPsaila, Bethan. "Interactions between megakaryocytes and tumour cells in the bone marrow vascular stem cell niche promote tumour growth and metastasis". Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/5945.
Texto completoGardner, M. J. "Adhesion molecules in the interactions of ovarian tumour cells and mesothelial cells". Thesis, University of Newcastle Upon Tyne, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336754.
Texto completoBillström, Anita. "The significance of urokinase-type plasminogen activator (u-PA) in tumour growth and linomide-induced upregulation of u-PA's endogenous inhibitor PAI-2". Lund : Research Laboratory, Dept. of Obstetrics and Gynaecology, Lund University Hospital, 1997. http://catalog.hathitrust.org/api/volumes/oclc/39751812.html.
Texto completoPu, J. "Electrical signals control the establishment of cell polarity, tumour metastasis and wound healing". Thesis, University of Aberdeen, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.590979.
Texto completoAl-Mulla, Fahd. "Genetic aberrations associated with metastasis in colorectal cancer : an insight into tumour heterogeneity". Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300772.
Texto completoOates, Adam John. "The identification of metastasis-related gene products in a rodent mammary tumour model". Thesis, University of Liverpool, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283448.
Texto completoDurkan, Garrett Christopher. "Matrix metalloproteinase-1 and -9 and tissue inhibitor of metalloproteinase-1 in bladder cancer : pathophysiological significance and relationship to epidermal growth factor receptor expression". Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369832.
Texto completoSpeakman, H. "Metastasis of the LMC1̲ rodent tumour and the response to treatment with cyclophosphamide in combination with surgery". Thesis, University of Leeds, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378851.
Texto completoVallejo, Espi Maria de Lourdes. "Characterization of the mechanisms by which Carbonic Anhydrase IX facilitates tumour growth and metastasis". Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/52795.
Texto completoMedicine, Faculty of
Biochemistry and Molecular Biology, Department of
Graduate
Taniguchi, Tadaaki. "Investigation of the mechanism of enhancement of invasion and tumour growth by tissue factor in human cancer". Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325327.
Texto completoHall, Debbie M. S. "Investigation of the role of N-acetylgalactosylated glycoconjugates in cancer metastasis using the lectin from Helix pomatia (the Roman snail)". Thesis, Oxford Brookes University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367328.
Texto completoNduka, Charles. "Operative dissemination of cancer : the impact of microenvironmental manipulation on post-operative tumour growth". Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391634.
Texto completoHolzapfel, Boris Michael. "Tissue engineering of a morphologically and functionally intact humanized bone organ for bone metastasis research". Thesis, Queensland University of Technology, 2015. https://eprints.qut.edu.au/84810/18/84810%28thesis%29.pdf.
Texto completoNouhi, Zaynab. "Prolactin plays a dual role in breast cancer : promoting formation of breast tumour while inhibiting its metastasis". Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97983.
Texto completoElsharif, Amal A. M. "Functional Investigation of Dual αvβ3 and αllbβ3 Integrin Inhibition in Haematological and Solid Tumour Models". Thesis, University of Bradford, 2018. http://hdl.handle.net/10454/16883.
Texto completoThe Libyan Embassy; Omer Al Mukhtar University, Faculty of Medical Technology, Derna, Libya.
Zraikat, Manar Saleh Ali. "Development of in vitro models of invasion for the pharmacological investigation of small molecule inhibitors of tumour progression : development and validation of a 3-dimensional tumour spheroid invasion model to evaluate the pharmacological effects of novel small molecule β3 integrin antagonists". Thesis, University of Bradford, 2015. http://hdl.handle.net/10454/7511.
Texto completoPittman, Kenneth Brian. "The development and evaluation of molecular biological techniques to detect solid tumour cells in peripheral blood /". Title page, table of contents and abstract only, 1995. http://web4.library.adelaide.edu.au/theses/09MD/09mdp689.pdf.
Texto completoJassim, Amir. "Involvement of the matrix proteins SPARC and osteopontin in the dynamic interaction between tumour and host cells". Thesis, Brunel University, 2016. http://bura.brunel.ac.uk/handle/2438/13399.
Texto completoElkashef, Sara M. "Pharmacological evaluation of novel polysialyltransferase inhibitors as anti-metastatic agents and development of analytical methods for assessment of polysialylation inhibition : in vitro assessment of the effects of novel polysialyltransferase inhibitors on tumour cell function and development of quantitative HPLC-based methods for evaluation of novel polysialyltransferase inhibitors". Thesis, University of Bradford, 2016. http://hdl.handle.net/10454/14123.
Texto completoPhipps, Laura Ellen. "Targeting cell adhesion as a method of sensitising metastatic tumour cells to TRAIL-induced apoptosis". Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:93198943-a7fa-4b30-aaed-c92d7e0a4ba6.
Texto completoRecalde-Percaz, Leire. "Neuropilin 2 role in the regulation of disseminated tumour cells dormancy and metastasis in breast and head and neck cancer". Doctoral thesis, Universitat de Barcelona, 2021. http://hdl.handle.net/10803/672374.
Texto completoLes metàstasis, principal causa de mortalitat associada a tumors sòlids que provoquen la mort de més del 90% dels pacients, no disposen d’un tractament efectiu. Les metàstasis deriven de cèl·lules tumorals disseminades (CTDs) que escapen del tumor primari i, després d’un període d’adaptació al nou microambient, proliferen donant lloc a la metàstasi. Durant aquest període d’adaptació, les CTDs entren en un estat de latència que es caracteritza per una parada del cicle cel·lular, fet que les fa invisibles davant de les teràpies actuals. Mitjançant mecanismes parcialment desconeguts, les CTDs es reactiven i proliferen generant la metàstasi. Per tant, és imprescindible millorar el coneixement sobre la biologia de les CTDs per fer front a un dels principals problemes de l’oncologia clínica actual. Per tal que es generi una metàstasi, es requereix la supervivència de les CTDs tant en circulació com al nou microambient que colonitzaran. Tenint tot això en compte, proposem que el microambient tumoral influencia el fenotip i la supervivència de les CTDs, regulant així la formació de metàstasis. Aquesta tesi ha tingut com a objectiu l’estudi de la influència de factors neuronals en el desenvolupament del tumor i la metàstasi als càncers de mama i de cap i coll. Concretament, hem estudiat el paper de la Neuropilina 2 (NRP2) en la regulació de la biologia i supervivència de les CTDs, així com en la modulació del microambient tumoral, afavorint la formació de metàstasis. Així mateix, hem analitzat l’efecte i la contribució de les semaforines de classe 3 (SEMA3s) i els seus receptors, les plexines (PLXNs), als càncers de mama i de cap i coll. D’una banda, hem posat de manifest la funció anti-tumoral de la SEMA3F en augmentar l’expressió de marcadors de latència, a més de reduir la disseminació tumoral i d’induir fenotips menys proliferatius a les CTDs in vivo. D’altra banda, els nostres resultats suggereixen que la PLXNA2 semblaria inhibir el creixement de tumors de mama receptor d’estrogen (ER)-negatius, disminuint la migració i invasió cel·lular. Altrament, l’expressió de la PLXNA3 està regulada per la via dels estrògens, associant la seva expressió als períodes de latència més perllongats en tumors de mama ER-positius. Finalment, el nostre estudi s’ha centrat a determinar la funció de la NRP2 a les CTDs i al desenvolupament de metàstasis al pulmó. En concret, hem demostrat la relació de l’expressió de NRP2 amb una major proliferació, adhesió, migració, invasió i supervivència cel·lular in vitro i in vivo. En concordança, hem mostrat que la inhibició de NRP2 redueix tant el creixement del tumor primari com la formació de metàstasis als pulmons in vivo. Finalment, destacant el paper del microambient tumoral als pulmons, hem vist que el TGFβ1 secretat pels macròfags i fibroblasts pulmonars augmenta l’expressió de NRP2 a les cèl·lules tumorals. En definitiva, aquesta tesi contribueix a un major coneixement de les CTDs descrivint l’eix TGFβ1- NRP2 com a inhibidor de la latència cel·lular i promotor de les metàstasis pulmonars. Tenint en compte la correlació de l’expressió de NRP2 en pacients de càncer de mama i de cap i coll amb una major incidència de metàstasis, definim la NRP2 com a marcador de mala prognosi contra la qual generar possibles noves eines terapèutiques per a millorar els tractaments de la malaltia metastàtica
Toh, Alan Kie Leong. "Functional roles of EMP-associated targets in breast cancer models". Thesis, Queensland University of Technology, 2021. https://eprints.qut.edu.au/207818/1/Alan%20Kie%20Leong_Toh_Thesis.pdf.
Texto completoHam, Sunyoung. "The role of breast cancer derived-exosomes in the tumour micro-environment". Thesis, Queensland University of Technology, 2021. https://eprints.qut.edu.au/208432/1/Sunyoung_Ham_Thesis.pdf.
Texto completoGrawenda, Anna Maria. "The identification and analysis of molecular biomarkers in the p53 tumour suppressor pathway that affect cancer progression in humans". Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:5a76b7ca-22f6-4f49-b715-5ad43f916984.
Texto completoHassan, Sara. "Epithelial-mesenchymal plasticity in circulating tumour cells from patients with metastatic cancers and PDX models". Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/228621/8/Sara_Hassan_Thesis.pdf.
Texto completoBrown, David Norman. "Application of phylogenetic inference methods to quantify intra-tumour heterogeneity and evolution of breast cancers". Doctoral thesis, Universite Libre de Bruxelles, 2017. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/260251.
Texto completoDoctorat en Sciences biomédicales et pharmaceutiques (Médecine)
info:eu-repo/semantics/nonPublished
Luco, Aimee-Lee. "Vitamin D strongly influences skeletal metastasis development in breast cancer: comparison of systemic vitamin D deficiency versus local ablation of CYP27B1 in breast tumour cells". Thesis, McGill University, 2014. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=121223.
Texto completoLa vitamine D est bien connue pour son rôle dans le maintien des concentrations de calcium et du phosphore dans la circulation ainsi que dans la prévention du rachitisme. La découverte plus récente de sa capacité d'inhiber la prolifération cellulaire, induire leur différentiation ainsi que l'apoptose cellulaire, inhiber l'angiogenèse, et moduler le système immunitaire rend son étude un sujet de recherche très intéressant surtout dans le domaine de la recherche sur le cancer. Nous avons étudié l'effet de la carence en vitamine D sur la croissance tumorale dans un modèle murin de métastases osseuses du cancer du sein. Nous avons aussi établi que ces cellules expriment l'enzyme CYP27B1 (1α-hydroxylase) et sont donc capables d'activer la vitamine D en son métabolite actif la 1,25-dihydroxyvitamine D (1,25(OH)2D) à partir du métabolite inactif, la 25-hydroxyvitamine D (25(OH)D). Nous avons ensuite examiné l'effet de l'activation locale de la vitamine D par les cellules tumorales dérivées du sein sur la croissance de ces cellules dans le microenvironnement osseux. Nous n'avons constaté aucune différence significative entre la croissance des cellules tumorales du cancer du sein dans l'os chez les souris carencées en vitamine D en comparaison aux souris non carencées en vitamine D. Cependant, nous avons démontré que les cellules tumorales du cancer du sein qui expriment le CYP27B1 croissent beaucoup moins vite dans l'os que les cellules tumorales qui n'expriment pas le CYP27B1. Ces résultats suggèrent un rôle très important de l'activation extra-rénale de la vitamine D par les cellules tumorales du cancer du sein pour inhiber la croissance de ces cellules dans l'os. En conclusion, ces travaux indiquent que le précurseur inactif 25(OH)D pourrait être utilisé seul ou en combinaison pour le traitement des métastases osseuses du cancer du sein.
Al, Saihati Hajir. "SIP1/ZEB2-induced epithelial mesenchymal transition promotes metastasis and alters chemokine (C-C motif) ligand 5 expression to modulate the tumour microenvironment in colorectal cancer". Thesis, University of Southampton, 2016. https://eprints.soton.ac.uk/417395/.
Texto completoLai, Andrew. "Towards the development of novel bispecific antibodies to inhibit key cell surface receptors integral for the growth and migration of tumour cells". Thesis, Queensland University of Technology, 2016. https://eprints.qut.edu.au/101339/1/Andrew_Lai_Thesis.pdf.
Texto completoMinutentag, Iael Weissberg. "Identificação de alterações no transcritoma associadas à progressão metastática em adenocarcinoma de reto". Botucatu, 2019. http://hdl.handle.net/11449/180847.
Texto completoResumo: Introdução: Apesar dos avanços no tratamento, cerca da metade dos pacientes com câncer de reto (CR) desenvolverá metástase à distância. No entanto, as vias biológicas envolvidas na progressão do câncer não são totalmente conhecidas. Neste estudo, investigamos os perfis moleculares e imunológicos em adenocarcinomas de reto relacionados à progressão metastática visando identificar biomarcadores moleculares e/ou alvos terapêuticos. Pacientes e Métodos: O transcritoma de 15 tecidos de CR metastático (M) e não-metastático (NM) pré-tratamento e de duas amostras de tecido de reto normais foi avaliado utilizando a plataforma Clariom D. Os genes foram considerados diferencialmente expressos quando a alteração de expressão era maior que 2 vezes e o valor de p <0,05 e detectados com o pacote limma. As funções moleculares e vias biológicas foram determinadas com a ferramenta Enricher. Os achados foram validados utilizando dados do TCGA e o perfil imunológico determinado com o algorótimo xCell. Resultados: A comparação entre os grupos M e NM revelou 52 genes diferencialmente expressos, sendo 27 regulados positivamente e 25 regulados negativamente. O gene ANLN foi detectado com o maior valor de fold change nos tumores metastáticos. Além disso, expressão aumentada de ANLN foi associada com menor sobrevida em pacientes com CR. A via do fator de crescimento endotelial vascular (VEGF) foi detectada como alterada nos tumores M. Validação dos resultados com dados do TCGA confirmou o gene ANLN co... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Introduction: Despite advances in treatment, about half of patients with rectal cancer (RC) will develop distant metastasis. However, the biological pathways underpinning the cancer progression are not fully understood. In this study, we sought to identify molecular and immunological profiles in rectal adenocarcinomas related to metastatic progression aiming to identify molecular biomarkers and/or therapeutic targets. Patients and Methods: Transcriptome analysis of 15 pre-treatment metastatic (M) and non-metastatic (NM) rectal cancer tissues and two normal rectal tissue samples was evaluated using Clariom D platform. Genes were considered differentially expressed when presenting 2-fold change and p<0.05 and were obtained with limma package . Molecular function and biological pathways with the Enricher package. Our findings were validated from the TCGA database and the immunological profile was determined using the xCell algorithm. Results: The comparison of M with NM groups revealed 52 differentially expressed genes, being 27 up-regulated and 25 down-regulated. ANLN gene was detected as the top gene upregulated in M tumours. Additionally, ANLN overexpression was associated with shorter survival in RC patients. Vascular endothelial growth factor (VEGF) pathway was detected as altered in M tumours. Cross-study validation with TCGA dataset confirmed ANLN gene as associated with M tumours. Furthermore, KIF14, XRCC2 and GPX3 genes, which have important carcinogenesis functions, we... (Complete abstract click electronic access below)
Mestre
Tomasin, Rebeka 1985. "Estudo da evolução tumoral, caquexia e metástase em diferentes modelos animais in vivo e in vitro = Tumour growth, cachexia and metastasis in vivo and in vitro". [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317753.
Texto completoTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-26T00:25:10Z (GMT). No. of bitstreams: 1 Tomasin_Rebeka_D.pdf: 4368331 bytes, checksum: 33a1f49902e0d1a423b1a4b7ebe757e9 (MD5) Previous issue date: 2014
Resumo: Câncer é um nome genérico para um grupo de mais de cem doenças que compartilham algumas características. Talvez a característica mais marcante das neoplasias malignas seja a rápida proliferação de células anormais para além de suas fronteiras usuais, invasão de tecidos adjacentes e finalmente dispersão para órgãos distantes. Anualmente, cerca de oito milhões de pessoas morrem devido ao câncer e outros doze milhões de novos casos são diagnosticados. Dentre os eventos associados à progressão neoplásica, destacam-se as metástases e a caquexia. Metástases são tumores em sítios secundários, sendo responsáveis por cerca de 90% do total de mortes por câncer. Já a caquexia, uma síndrome paraneoplásica, é caracterizada por extensa espoliação de gordura e massa magra, fadiga e alterações metabólicas, prejudicando a qualidade de vida e a sobrevida de cerca de 50-85% dos pacientes, dependendo do tipo de tumor. Com relação às terapias, o grande desafio ainda é combater o tumor sem prejudicar o hospedeiro, o que acredita-se ser possível através de terapias-alvo para genes específicos e/ou tratamentos coadjuvantes, incluindo aqueles que empregam suplementação e/ou drogas derivadas de produtos naturais, que muitas vezes tem menor efeito tóxico ao hospedeiro. Desse modo, este trabalho teve dois objetivos: (1) avaliar a ação da administração oral de Aloe vera e mel sobre o tecido tumoral e o hospedeiro portador de carcinosarcoma de Walker 256; e (2) identificação de genes supressores de metástase através de screen funcional in vivo empregando-se biblioteca de shRNA em modelo de câncer de mama triplo negativo. Em relação ao primeiro objetivo, os resultados sugerem que a associação entre Aloe vera e mel pode modular a proteólise e o estresse oxidativo de maneira diferencial preservando o hospedeiro em detrimento do tecido tumoral. Ainda, o tratamento com Aloe vera e mel parece ser capaz de diminuir a propensão metastática das células tumorais in vivo, através de aumento na expressão de caderina-E e redução na expressão de caderina-N, bem como inibição da angiogênese. Outros experimentos sugerem que os efeitos antitumorais observados in vivo estão, em parte, relacionados à ação imunomodulatória de alguns componentes da Aloe vera. Com relação ao segundo objetivo, foram identificados dezenas de candidatos a genes supressores de metástase. Dentre esses genes, que estão sendo validados, Mnat1, Snd1, Cul5, Gabbr1, Rorb, Adk, Ccnd3, Gdnf, Nr1d1, Ptprs e Ltah4 são os genes-candidatos de maior confiabilidade por cumprirem um ou mais dos seguintes requisitos: (a) diminuição significativa do nível de DNA e RNA em canceres de mama humanos agressivos, sendo assim relacionados à pior prognostico, (b) papel biológico sugestivo, (c) fenótipo marcante durante o screen ou ainda (d) decréscimo significativo na expressão em linhagens de câncer de mama mais agressivas
Abstract: Cancer is a generic name for a group of over a hundred diseases which share some features. The most remarkable feature in cancer disease is the fast proliferation of abnormal cells beyond their usual boundaries, invasion of surrounding tissues and finally spread to distant organs. Every year, cancer is responsible for over eight million deaths and twelve million new cases are diagnosed. Among all the events associated with the neoplastic progression, metastasis and cachexia are major issues. Metastases, which are tumours growing in secondary sites, account for 90% of all cancer deaths. Cachexia, a paraneoplastic syndrome, is characterized by severe fat and lean mass waste, fatigue and metabolic alterations, jeopardizing the quality of life and reducing the survival of about 50-85% of the cancer patients, depending on the tumour type. Regarding to the therapies, the biggest challenge is still fighting the tumour without harming the host, which is believed to be possible by targeted therapies to specific genes and/or adjuvant treatments, including supplementation and drugs derived from natural compounds, which usually have lower side effects in the host. Knowing these points, this work had two aims: (1) evaluate the effects of Aloe vera and honey in both tumour and host tissues in Walker 256-tumour bearing rats; and, (2) identification of metastasis suppressor genes using a functional in vivo shRNA screen in a triple negative breast cancer syngeneic model. Regarding to the first aim, the results suggested that the combination of Aloe vera and honey selectively modulate proteolysis and oxidative stress, damaging the tumour tissue while protected the host. Moreover, the Aloe vera and honey treatment apparently decreases the metastatic potential in vivo, by simultaneous increase in E-cadherin and decrease in N-cadherin expression, while decreased tumour vascularization. Finally, our results suggested the antitumoral effects observed in vivo are, at least partially, related to the immunomodulatory activity of some Aloe vera¿s compounds. Regarding to the second aim, dozens of putative metastasis suppressor genes were identified. High confidence candidates, which would be further analysed are Mnat1, Snd1, Cul5, Gabbr1, Rorb, Adk, Ccnd3, Gdnf, Nr1d1, Ptprs e Ltah4. Their selection was based on meeting the following requirements: (a) significant decrease at DNA or RNA level in highly aggressive human breast cancer carcinomas and thus, worse prognosis, (b) suggestive biological role, (c) occurrence of a remarkable phenotype during the screen, and (d) significant decrease in expression in more aggressive cancer cell lines
Doutorado
Biologia Celular
Doutora em Biologia Celular e Estrutural
Mallet, Daniel Gordon. "Mathematical Modelling of the Role of Haptotaxis in Tumour Growth and Invasion". Queensland University of Technology, 2004. http://eprints.qut.edu.au/15941/.
Texto completoKrishnan, Jaya. "The role of vascular endothelial growth factor receptor 3, and its ligands vascular endothelial growth factor C and vascular endothelial growth factor D in tumour metastasis and haematopoeisis". Thesis, University of London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270576.
Texto completo