Bibliografías temáticas / Transgenic grapevine

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Artículos de revistas

Literatura académica sobre el tema "Transgenic grapevine"

Autor: Grafiati

Publicado: 11 de marzo de 2023

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Artículos de revistas sobre el tema "Transgenic grapevine"

Gölles, R., R. Moser, H. Pühringer, et al. "TRANSGENIC GRAPEVINES EXPRESSING COAT PROTEIN GENE SEQUENCES OF GRAPEVINE FANLEAF VIRUS, ARABIS MOSAIC VIRUS, GRAPEVINE VIRUS A AND GRAPEVINE VIRUS B." Acta Horticulturae, no. 528 (May 2000): 307–14. http://dx.doi.org/10.17660/actahortic.2000.528.42.

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Yu, Yanyan, Yong Ni, Tian Qiao, et al. "Overexpression of VvASMT1 from grapevine enhanced salt and osmotic stress tolerance in Nicotiana benthamiana." PLOS ONE 17, no. 6 (2022): e0269028. http://dx.doi.org/10.1371/journal.pone.0269028.

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Salt and drought stresses are major environmental conditions that severely limit grape growth and productivity, while exogenous melatonin can alleviate the drought and salt damage to grapevines. N-acetylserotonin methyltransferase (ASMT) is the key enzyme in melatonin synthesis, which plays a critical role in regulating stress responses. However, the roles of ASMTs from grapevine under drought and salt stresses responses remain largely unclear. In this study, the VvASMT1 gene was isolated from grapevine, and its physiological functions in salt and mimic drought stress tolerance were investigated. Expression pattern analysis revealed that VvASMT1 was significantly induced by different salt and osmotic stresses. Ectopic expression of VvASMT1 in Nicotiana benthamiana significantly enhanced melatonin production in transgenic plants. Compared with wild-type plants, the transgenic lines exhibited a higher germination ratio, longer root length, lower degree of leaf wilting and relative water content (RWC) under salt and osmotic stresses. In addition, under salt and osmotic stresses, overexpression of VvASMT1 improved proline and malondialdehyde (MDA) contents, increased the activity of antioxidant enzymes and decreased the accumulation of reactive oxygen species (ROS). Taken together, our results demonstrate the explicit role of VvASMT1 in salt and osmotic stress responses, which provides a theoretical foundation for the genetic engineering of grapevine.

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Gribaudo, I., G. Gambino, S. Leopold, and M. Laimer. "MOLECULAR CHARACTERIZATION OF TRANSGENIC GRAPEVINE PLANTS." Acta Horticulturae, no. 689 (August 2005): 485–92. http://dx.doi.org/10.17660/actahortic.2005.689.59.

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Levenko, B. A., and M. A. Rubtsova. "HERBICIDE RESISTANT TRANSGENIC PLANTS OF GRAPEVINE." Acta Horticulturae, no. 528 (May 2000): 339–42. http://dx.doi.org/10.17660/actahortic.2000.528.46.

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Gray, D. J., Z. T. Li, D. L. Hopkins, et al. "Transgenic Grapevines Resistant to Pierce's Disease." HortScience 40, no. 4 (2005): 1104D—1105. http://dx.doi.org/10.21273/hortsci.40.4.1104d.

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Pierce's disease (PD), caused by the xylem-limited bacterium Xylella fastidiosa, is endemic to the coastal plain of the southeastern United States. Although native southern grapevines are tolerant to X. fastidiosa, all varieties of Vitisvinifera grown in the region will succumb to PD. Genetic transformation to add disease resistance genes, while not disturbing desirable phenotypic characters, holds promise for expanding the southeastern U.S. grape industry by allowing use of established fruit and wine varieties. We utilize embryogenic cell cultures and Agrobacterium strain EHA105 to refine transformation systems for Vitis species and hybrids. V. vinifera`Thompson Seedless' is employed as a model variety to test various transgenes for disease resistance, since as many as 150 independent transgenic plant lines routinely are produced from 1 g of embryogenic culture material. Transgenic plants are stringently screened for PD resistance in greenhouses by mechanical inoculation with X. fastidiosa. Transgenic plants are compared with both susceptible and resistant control plants by assessing typical PD symptom development and by assaying bacterial populations in xylem sap over time. Using these procedures, nine putative PD resistance genes have been inserted into grapevine and over 900 unique transgenic lines have been evaluated. A range of susceptible-to-resistant responses has been catalogued. Thus far, the best construct for PD resistance contains a grape codon-optimized hybrid lytic peptide gene in a high-performance bi-directional 35S promoter complex. Certain transgenic plant lines containing this construct exhibit better resistance than that of resistant control vines.

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Krastanova, S., K. S. Ling, H. Y. Zhu, B. Xue, T. J. Burr, and D. Gonsalves. "DEVELOPMENT OF TRANSGENIC GRAPEVINE ROOTSTOCKS WITH GENES FROM GRAPEVINE FANLEAF VIRUS AND GRAPEVINE LEAFROLL ASSOCIATED CLOSTEROVIRUSES 2 AND 3." Acta Horticulturae, no. 528 (May 2000): 367–72. http://dx.doi.org/10.17660/actahortic.2000.528.52.

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Dutt, Manjul, Dennis J. Gray, Zhijian T. Li, Sadanand Dhekney, and Marilyn M. Van Aman. "Micropropagation Cultures for Genetic Transformation of Grapevine." HortScience 41, no. 4 (2006): 972C—972. http://dx.doi.org/10.21273/hortsci.41.4.972c.

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A major drawback to the use of embryogenic cultures for transformation of grapevine is that their ability to undergo genetic transformation is cultivar-dependent. Also, depending on cultivar, embryogenic cultures are difficult to impossible to maintain over time, reducing their utility for use in genetic transformation. An alternative to the use of embryogenic cultures for transformation of grapevine is the use of micropropagation cultures, which are easier to initiate from a wide range of grapevine cultivars and can be maintained over time without loss of function. Vitis vinifera `Thompson Seedless' was used as a model for genetic transformation using micropropagation cultures. In vitro cultures were initiated from apical meristems of actively growing vines and maintained in C2D medium containing 4 μM of 6-benzylaminopurine (C2D4B). Shoot tips and nodes were collected from proliferating in vitro cultures for transformation studies. A variety of wounding techniques, including nicking, sonication, and fragmenting of meristematic tissues was employed in order to enable Agrobacterium infection. We used a construct containing a bidirectional 35S promoter complex with a marker gene composed of a bifunctional fusion between an enhanced green fluorescent protein (EGFP) gene and a neomycin phosphotransferase (NPTII) gene in one direction and a hybrid lytic peptide gene in the other. Transgenic shoots growing in C2D4B medium containing 200 mg·L-1 each of carbenicillin and cefotaxime and 20 mg·L-1 of kanamycin were selected based on GFP fluorescence. Transgenic shoots were rooted and transferred to a greenhouse. To date, 18 transgenic lines have been generated. Details on the transformation procedure will be discussed.

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Aleynova, Olga A., Konstantin V. Kiselev, Zlata V. Ogneva, and Alexandra S. Dubrovina. "The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response." International Journal of Molecular Sciences 21, no. 21 (2020): 7939. http://dx.doi.org/10.3390/ijms21217939.

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Calmodulin-like proteins (CMLs) represent a large family of plant calcium sensor proteins involved in the regulation of plant responses to environmental cues and developmental processes. In the present work, we identified four alternatively spliced mRNA forms of the grapevine CML21 gene that encoded proteins with distinct N-terminal regions. We studied the transcript abundance of CML21v1, CML21v2, CML21v3, and CML21v4 in wild-growing grapevine Vitis amurensis Rupr. in response to desiccation, heat, cold, high salinity, and high mannitol stress using quantitative real-time RT-PCR. The levels of all four splice variants of VaCML21 were highly induced in response to cold stress. In addition, VaCML21v1 and VaCML21v2 forms were highly modulated by all other abiotic stress treatments. Constitutive expression of VaCML21v2 and VaCML21v4 improved biomass accumulation of V. amurensis callus cell cultures under prolonged low temperature stress. Heterologous expression of the grapevine CML21v2 and VaCML21v4 splice variants in Arabidopsis improved survival rates of the transgenic plants after freezing. The VaCML21v2 overexpression enhanced activation of the cold stress-responsive marker genes AtDREB1A and AtDREB2A, while VaCML21v4 overexpression—AtCOR47, AtRD29A, AtRD29B, and AtKIN1 genes after freezing stress in the transgenic Arabidopsis. The results indicate that the grapevine CML21 gene acts as a positive regulator in the plant response to cold stress. The detected variety of CML21 transcripts and their distinct transcriptional responses suggested that this expansion of mRNA variants could contribute to the diversity of grapevine adaptive reactions.

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Li, Wei, Changxi Dang, Yuxiu Ye, et al. "Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco." International Journal of Molecular Sciences 21, no. 4 (2020): 1323. http://dx.doi.org/10.3390/ijms21041323.

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In plants, auxin/indoleacetic acid (Aux/IAA) proteins are transcriptional regulators that regulate developmental process and responses to phytohormones and stress treatments. However, the regulatory functions of the Vitis vinifera L. (grapevine) Aux/IAA transcription factor gene VvIAA18 have not been reported. In this study, the VvIAA18 gene was successfully cloned from grapevine. Subcellular localization analysis in onion epidermal cells indicated that VvIAA18 was localized to the nucleus. Expression analysis in yeast showed that the full length of VvIAA18 exhibited transcriptional activation. Salt tolerance in transgenic tobacco plants and Escherichia. coli was significantly enhanced by VvIAA18 overexpression. Real-time quantitative PCR analysis showed that overexpression of VvIAA18 up-regulated the salt stress-responsive genes, including pyrroline-5-carboxylate synthase (NtP5CS), late embryogenesis abundant protein (NtLEA5), superoxide dismutase (NtSOD), and peroxidase (NtPOD) genes, under salt stress. Enzymatic analyses found that the transgenic plants had higher SOD and POD activities under salt stress. Meanwhile, component analysis showed that the content of proline in transgenic plants increased significantly, while the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) decreased significantly. Based on the above results, the VvIAA18 gene is related to improving the salt tolerance of transgenic tobacco plants. The VvIAA18 gene has the potential to be applied to enhance plant tolerance to abiotic stress.

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Rubtsova, M. A., and B. A. Levenko. "PHOSPHINOTHRICIN- AND CROWN GALL-RESISTANT TRANSGENIC PLANTS OF GRAPEVINE." Acta Horticulturae, no. 625 (September 2003): 465–72. http://dx.doi.org/10.17660/actahortic.2003.625.55.

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