Bibliografías temáticas / The Wild Wynchesters series

Literatura académica sobre el tema "The Wild Wynchesters series"

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Publicado: 27 de julio de 2024
Última modificación: 28 de julio de 2024

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Artículos de revistas sobre el tema "The Wild Wynchesters series"

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Kreiss, Jens-peter y Efstathios Paparoditis. "The Hybrid Wild Bootstrap for Time Series". Journal of the American Statistical Association 107, n.º 499 (4 de junio de 2012): 1073–84. http://dx.doi.org/10.1080/01621459.2012.695664.

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Cudney, David W., Lowell S. Jordan, Jodie S. Holt y John S. Reints. "Competitive Interactions of Wheat (Triticum aestivum) and Wild Oats (Avena fatua) Grown at Different Densities". Weed Science 37, n.º 4 (julio de 1989): 538–43. http://dx.doi.org/10.1017/s0043174500072374.

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Competitive interactions of wheat and wild oats in relation to variations in plant density of both species were investigated in field studies under irrigation and added fertilizer. Competition studies included comparisons by both additive and replacement series. The additive series was used to study plant responses to competition under fixed density of wheat and increasing density of wild oats. The replacement series was used to study plant responses to competition under constant total plant density with differing proportions of wheat and wild oats. On a per plant basis, shoot dry weight and leaf area index of wild oats were less than those of wheat at anthesis. However, the replacement series experiment indicated that wheat and wild oats were equivalent in competitiveness. Relative density of wild oats gave a better regression fit for wheat yield than did absolute wild oats density (r2values of 0.83 and 0.61, respectively). Yield of wheat grain was linearly proportional to relative density of wild oats.
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Church, S. A. y E. J. Spaulding. "Gene Expression in a Wild Autopolyploid Sunflower Series". Journal of Heredity 100, n.º 4 (25 de marzo de 2009): 491–95. http://dx.doi.org/10.1093/jhered/esp008.

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Baid, Anisha. "Wild Life". Membrana Journal of Photography, Vol. 3, no. 1 (2018): 20–25. http://dx.doi.org/10.47659/m4.020.art.

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Wild Life is a series of augmented photographs of animals and insects placed in vacant, overgrown spaces in suburban Bangalore. Taken through mobile AR apps like Holo and Augment, these photographs (or screenshots) situate virtual bodies within the frame of the mobile camera – creating something in between a document and fiction. The work investigates these processes of augmentation, which enable 3D representations of things in the real/physical world to be projected back into physical space that are then photographed. The larger phenomenon of AR photography also complicates traditional notions of “immersive” media – forcing one to interact with their environments. This essay reflects on the implications of mobile AR photography on the image and the referent. Through a phenomenological reading of and immersion into popular uses of mobile AR (like the game Pokémon Go), the essay is an observation of the convoluted relationships evoked between augmented bodies, their environments and the screens on which they manifest. Keywords: digital image, documentary, mobile AR, photography, Pokémon Go
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Zheglov, A. B. "On wild division algebras over fields of power series". Sbornik: Mathematics 195, n.º 6 (30 de junio de 2004): 783–817. http://dx.doi.org/10.1070/sm2004v195n06abeh000825.

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Morishita, Don W., Donald C. Thill y John E. Hammel. "Wild Oat (Avena fatua) and Spring Barley (Hordeum vulgare) Interference in a Greenhouse Experiment". Weed Science 39, n.º 2 (junio de 1991): 149–53. http://dx.doi.org/10.1017/s0043174500071381.

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Intraspecific and interspecific interference effects on growth, gas exchange, and water potential of wild oat and spring barley were measured under greenhouse conditions using a 1:1.06 barley to wild oat replacement series. Intraspecific barley interference affected barley growth more than interspecific wild oat interference. Interspecific wild oat interference with barley reduced wild oat growth more than intraspecific interference. Wild oat plant height surpassed barley plant height near barley anthesis. Growth and gas exchange of barley and wild oat responded the same to short-term water stress.
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Castillo, Raul O. y David M. Spooner. "Phylogenetic Relationships of Wild Potatoes, Solanum Series Conicibaccata (Sect. Petota)". Systematic Botany 22, n.º 1 (enero de 1997): 45. http://dx.doi.org/10.2307/2419677.

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Auer, Richard E. y Michael P. Knapp. "Delving Deeper: Studying Baseball's Wild-Card Team Using Probability". Mathematics Teacher 107, n.º 1 (agosto de 2013): 74–77. http://dx.doi.org/10.5951/mathteacher.107.1.0074.

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The modern era of professional baseball playoffs began in 1903, when the champions of the American League and the National League played the first World Series. Except for one year, 1904, this playoff system was maintained until 1969. Beginning in 1969, each of the two leagues in Major League Baseball (MLB) was divided into two divisions to accommodate the addition of extra teams in each league. From then through 1993, the two divisional champions in each league played against each other in an initial playoff round to determine which teams would go on to the World Series.
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Wall, David A. "Comparative Analysis of Three Cruciferous Weeds: Growth, Development, and Competitiveness". Weed Science 43, n.º 1 (marzo de 1995): 75–80. http://dx.doi.org/10.1017/s0043174500080851.

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Wild, ball, and dog mustard growth and development were investigated by mathematical growth analysis in a greenhouse experiment. Plant height and total plant biomass over the growth period followed the trend wild mustard > ball mustard > dog mustard. Dog mustard plants had lower leaf areas than either wild or ball mustard. In a replacement series experiment, wild mustard was more competitive than either ball or dog mustard, and ball mustard was more competitive than dog mustard.
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Huang, Hang, Mateusz Michałek y Emanuele Ventura. "Vanishing Hessian, wild forms and their border VSP". Mathematische Annalen 378, n.º 3-4 (14 de septiembre de 2020): 1505–32. http://dx.doi.org/10.1007/s00208-020-02080-8.

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Abstract Wild forms are homogeneous polynomials whose smoothable rank is strictly larger than their border rank. The discrepancy between these two ranks is caused by the difference between the limit of spans of a family of zero-dimensional schemes and the span of their flat limit. For concise forms of minimal border rank, we show that the condition of vanishing Hessian is equivalent to being wild. This is proven by making a detour through structure tensors of smoothable and Gorenstein algebras. The equivalence fails in the non-minimal border rank regime. We exhibit an infinite series of minimal border rank wild forms of every degree $$d\ge 3$$ d ≥ 3 as well as an infinite series of wild cubics. Inspired by recent work on border apolarity of Buczyńska and Buczyński, we study the border varieties of sums of powers $$\underline{{\mathrm {VSP}}}$$ VSP ̲ of these forms in the corresponding multigraded Hilbert schemes.
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Tesis sobre el tema "The Wild Wynchesters series"

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Duras, Toni. "Robust critical values for unit root tests for series with conditional heteroskedasticity errors using wild bootstrap". Thesis, Örebro universitet, Handelshögskolan vid Örebro Universitet, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-29781.

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Englund, Jonas. "Testing for Cointegration in Multivariate Time Series : An evaluation of the Johansens trace test and three different bootstrap tests when testing for cointegration". Thesis, Örebro universitet, Handelshögskolan vid Örebro Universitet, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-30067.

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In this paper we examine, by Monte Carlo simulation, size and power of the Johansens trace test when the error covariance matrix is nonstationary, and we also investigate the properties of three different bootstrap cointegration tests. Earlier studies indicate that the Johansen trace test is not robust in presence of heteroscedasticity, and tests based on resampling methods have been proposed to solve the problem. The tests that are evaluated is the Johansen trace test, nonparametric bootstrap test and two different types of wild bootstrap tests. The wild bootstrap test is a resampling method that attempts to mimic the GARCH model by multiplying each residual by a stochastic variable with an expected value of zero and unit variance. The wild bootstrap tests proved to be superior to the other tests, but not as good as earlier indicated. The more the error terms differs from white noise, the worse these tests are doing. Although the wild bootstrap tests did not do a very bad job, the focus of further investigation should be to derive tests that does an even better job than the wild bootstrap tests examined here.
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Castillo, Raúl Oswaldo. "Phylogenetic relationships of wild potatoes, Solanum series Conicibaccata (sec. Petota)". 1995. http://catalog.hathitrust.org/api/volumes/oclc/33869212.html.

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Thesis (Ph. D.)--University of Wisconsin--Madison, 1995.
Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Ames, Sevillano Mercedes I. "Evolutionary relationships of Solanum series Piurana and related species in Solanum section Petota (wild potatoes)". 2008. http://www.library.wisc.edu/databases/connect/dissertations.html.

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Villamon, Francisco G. "Late blight resistance linkages in a novel cross of the wild potato species Solanum paucissectum (series Piurana)". 2005. http://catalog.hathitrust.org/api/volumes/oclc/61325932.html.

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Thesis (M.S.)--University of Wisconsin--Madison, 2005.
Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 25-32)
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Fajardo, Diego Alberto. "Phylogenetic analysis of wild potato species, Solanum series Conicibaccata by morphology and conserved orthologous set II (COSII) markers". 2008. http://www.library.wisc.edu/databases/connect/dissertations.html.

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Libros sobre el tema "The Wild Wynchesters series"

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Wild Honey: Something Wild - 1, Man of the Month - 57. Richmond: Silhouette, 1994.

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The Governess Gambit: A Wild Wynchesters Prequel - The Wild Wynchesters - 0.5. WebMotion, 2021.

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The Duke Heist: The Wild Wynchesters - 1. Grand Central Publishing, 2021.

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The Duke Heist: The Wild Wynchesters - 1. Grand Central Publishing, 2021.

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The Rake Mistake: Heist Club - 1, The Wild Wynchesters - 1.5. Intrepid Reads, 2022.

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The Modiste Mishap: Heist Club - 2, The Wild Wynchesters - 2.5. Intrepid Reads, 2022.

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The Perks of Loving a Wallflower: The Wild Wynchesters - 2. Grand Central, 2021.

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Fergus, Charles. Turtles: Wild Guide (Wild Guides Series) (Wild Guide Series). Stackpole Books, 2007.

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Wild Wave: The Wild Series. Scholastic, Incorporated, 2024.

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Emert, Phyllis R. Wild Wings Series. Silver Burdett Press, 1990.

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Capítulos de libros sobre el tema "The Wild Wynchesters series"

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Masini, Francesca y Simone Mattiola. "Wild words". En Studies in Language Companion Series, 234–54. Amsterdam: John Benjamins Publishing Company, 2022. http://dx.doi.org/10.1075/slcs.223.10mas.

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Quitmeyer, Andrew y Kitty Kelly. "Wild Birthplaces of Behavioral Media". En Human–Computer Interaction Series, 99–118. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-45289-6_5.

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GERSHENZON, JONATHAN, MARYCAROL ROSSITER, TOM J. MABRY, CHARLIE E. ROGERS, MICHAEL H. BLUST y THEODORE L. HOPKINS. "Insect Antifeedant Terpenoids in Wild Sunflower". En ACS Symposium Series, 433–46. Washington, D.C.: American Chemical Society, 1985. http://dx.doi.org/10.1021/bk-1985-0276.ch030.

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Olsen, L. F., C. G. Steinmetz, C. W. Tidd y W. M. Schaffer. "Childhood Infections — Examples of ‘Chaos in the Wild’". En NATO ASI Series, 359–75. New York, NY: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-7847-1_26.

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Hall, J. Christopher, Nataraj N. Vettakkorumakankav y Hong-gang Zheng. "Auxinic Herbicide Resistance in Wild Mustard (Sinapis arvensisL.)". En ACS Symposium Series, 126–34. Washington, DC: American Chemical Society, 2001. http://dx.doi.org/10.1021/bk-2002-0808.ch007.

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Aal, Tanja y Margarita Grinko. "Breaking New Ground: Stories from the Wild". En Human–Computer Interaction Series, 139–52. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-31642-5_10.

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Klimis-Zacas, Dorothy y Aleksandra S. Kristo. "Wild Blueberries (Vaccinium angustifolium): Modulators of Vascular Function, Structure, and Metabolism". En ACS Symposium Series, 151–66. Washington, DC: American Chemical Society, 2012. http://dx.doi.org/10.1021/bk-2012-1093.ch009.

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Scognamiglio, Monica, Brigida D’Abrosca, Severina Pacifico, Marina Isidori, Assunta Esposito y Antonio Fiorentino. "Mediterranean Wild Plants As Useful Sources of Potential Natural Food Additives". En ACS Symposium Series, 209–35. Washington, DC: American Chemical Society, 2012. http://dx.doi.org/10.1021/bk-2012-1093.ch012.

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Guevara-Lara, F., L. G. Espinosa-Alonso, M. E. Valverde, A. Lygin, J. Widholm y O. Paredes-López. "Phenolics, Flavonoids and Other Nutraceuticals in Mexican Wild Common Beans (Phaseolus vulgaris)". En ACS Symposium Series, 77–88. Washington, DC: American Chemical Society, 2006. http://dx.doi.org/10.1021/bk-2007-0946.ch007.

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Sineiro, J., M. Rubilar, M. Cascante, E. Álvarez, M. Sánchez y M. J. Núñez. "Potential Therapeutic Applications of Common Agro-Food Byproducts and Chilean Wild Plants". En ACS Symposium Series, 117–30. Washington, DC: American Chemical Society, 2012. http://dx.doi.org/10.1021/bk-2012-1093.ch007.

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Actas de conferencias sobre el tema "The Wild Wynchesters series"

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V, Sri Sowmya, Sasikala D y Theetchenya S. "A Comparative Exploration of Time Series Models for Wild Fire Prediction". En 2024 Fourth International Conference on Advances in Electrical, Computing, Communication and Sustainable Technologies (ICAECT). IEEE, 2024. http://dx.doi.org/10.1109/icaect60202.2024.10469631.

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Okafor, Emmanuel, Pornntiwa Pawara, Faik Karaaba, Olarik Surinta, Valeriu Codreanu, Lambert Schomaker y Marco Wiering. "Comparative study between deep learning and bag of visual words for wild-animal recognition". En 2016 IEEE Symposium Series on Computational Intelligence (SSCI). IEEE, 2016. http://dx.doi.org/10.1109/ssci.2016.7850111.

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Zhang, HaiMing, JianHui Li, YuanChun Zhou, XueZhi Wang y BaoPing Yan. "Using a Time Series of Satellite Imagery to Study the Wild Birds' Migration". En 2013 Fourth International Conference on Networking and Distributed Computing (ICNDC). IEEE, 2013. http://dx.doi.org/10.1109/icndc.2013.34.

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Wilcoxen, Keith M., Rachael L. Brake, Doug Saffran, Yohannes Teffera, Deborah Choquette, Doug Whittington, Violeta Yu et al. "Abstract 1795: Characterization of a novel series of potent, selective inhibitors of wild type and mutant/fusion anaplastic lymphoma kinase". En Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-1795.

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Braga, Antonio, Lia Furtado, Antonio Bezerra, Breno Freitas, Joseph Cazier y Danielo Gomes. "Applying the Long-Term Memory Algorithm to Forecast Loss of Thermoregulation Capacity in Honeybee Colonies". En X Workshop de Computação Aplicada à Gestão do Meio Ambiente e Recursos Naturais. Sociedade Brasileira de Computação - SBC, 2019. http://dx.doi.org/10.5753/wcama.2019.6422.

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Bees are the main pollinators of most wild and cultivated plant species, thus being essential for the maintenance of plant ecosystems and for food production. But they are threatened due to a series of drivers such as pesticides, habitat loss and climate change. Here, we propose a method to iden- tify the loss of thermoregulation capacity in honeybee colonies. We applied the Long Short-Term Memory (LSTM) algorithm, which is based on Recurrent Neural Networks (RNN), to six real datasets of the Arnia remote hive monitoring system. From brood temperatures gathered along the European fall season in 2017, the LSTM was able to detect when a honeybee colony is about to lose its thermoregulation capacity. Our results showed an error of only 0.5% in predic- tion for well-thermoregulated beehives.
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Watts, Travis J., Jerry G. Rose y Ethan J. Russell. "Relationships Between Wheel/Rail Surface Impact Loadings and Correspondingly Transmitted Tie/Ballast Impact Pressures for Revenue Train Operations". En 2018 Joint Rail Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/jrc2018-6184.

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A series of specially designed granular material pressure cells were precisely positioned directly below the rail at the tie/ballast interface to measure typical interfacial pressures exerted by revenue freight trains. These vertical pressures were compared to the recorded wheel/rail nominal and peak forces for the same trains traversing nearby mainline wheel impact load detectors (WILDs). The cells were imbedded within the bottom of new wood ties so that the surfaces of the pressure cells were even with the bottoms of the ties and the underlying ballast. The cells were inserted below consecutive rail seats of one rail to record pressures for a complete wheel rotation. The stability and tightness of the ballast support influenced the magnitudes and consistencies of the recorded ballast pressures. Considerable effort was required to provide consistent ballast conditions for the instrumented ties and adjacent undisturbed transition ties. Norfolk Southern (NS) crews surfaced and tamped through the test section and adjacent approach ties. This effort along with normal accruing train traffic subsequently resulted in reasonably consistent pressure measurements throughout the test section. The impact ratio (impact factor) and peak force values recorded by the WILDs compared favorably with the resulting magnitudes of the transferred pressures at the tie/ballast interface. High peak force and high impact ratio WILD readings indicate the presence of wheel imperfections that increase nominal forces at the rail/wheel interface. The resulting increased dynamic impact forces can contribute to higher degradation rates for the track component materials and more rapid degradation rates of the track geometry. The paper contains comparative WILD force measurements and tie/ballast interfacial pressure measurements for loaded and empty trains. Typical tie/ballast pressures for locomotives and loaded freight cars ranges from 20 to 30 psi (140 to 210 kPa) for smooth wheels producing negligible impacts. The effect of increased wheel/rail impacts and peak force values on the correspondingly transmitted pressures at the tie/ballast interface is significant, with increased pressures of several orders of magnitude compared to nominal impact forces from wheels.
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"Impact of Heparan Sulphate Binding Domain of Chemokine CCL21 to Migration of Breast Cancer Cells". En Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0132.

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Lymph node metastasis constitutes a key event in breast cancer progression. Chemokines are small proteins, which can promote metastatic spread by inducing cancer cell migration and invasion. Chemokine function is dependant upon their binding to both cell surface heparan sulphate (HS) molecules and to their specific receptor. Our group has demonstrated a significant increase in chemokine receptor CCR7 expression in cancerous breast epithelia compared to healthy controls. This study is designed to test the hypothesis that a non-HS binding forms of chemokine CCL21 can disrupt the normal response to CCL21, therefore reducing the metastasis of CCR7-expressing cancer cells. Truncated CCL21 chemokine (Δ98- 134 c-terminal basic extension), was synthesised to investigate a possible linkage between chemokine binding capacity and cell activation. Wild type (WT) and mutant-CCL21 were tested for their ability to stimulate a dose-dependent increase in intracellular-free calcium in peripheral blood mononuclear cell (PBMC) and breast cancer epithelial cells MDA-MB-231. Mutant-CCL21 at concentrations 5 and 10nM showed potential to mobilise Ca2+ at levels similar to that produced by WT-CCl21. A series of experiments was performed to determine how deletion of the HS-binding site altered the ability of CCL21 to stimulate chemotaxis within a concentration gradient generated by free solute diffusion. PBMC stimulated to migrate by wild-type CCL21 was not significantly different from that stimulated by mutant (P> 0.05). Similar results were observed in assays using MDA-MB-231 cells. A further series of experiments was performed to compare the potential of WT and mutant-CCL21 to stimulate the migration of cells across endothelium. In contrast to results for trans-filter migration, it was found that the non HSbinding mutant stimulated no increased in transendothelial cell migration above the background at each of the tested concentrations, 10, 30 and 50 nM respectively (P>0.05). However, WT-CCL21 stimulated significant increased PBMC migration at each of the tested concentration (all P <0.001). Furthermore, the effect of heparin on chemotactic properties of WT and mutant- CCL21 was examined. Interestingly, heparin (250 µg/ml) completely inhibit the chemotaxis mediated by WT-CCL21 (5nM) (P < 0.001), whereas it did not inhibit the chemotaxis at concentrations 100, 250 & 500 µg/ml in response to mutant CCL21 (5nM) (P > 0.05). Similar assay will be performed using MDA-MB-231 cells. Work is ongoing to characterise the biophysical properties of mutant-CCL21 and determine its potential role for a therapeutic blockade of the migration of breast cancer cells in-vivo. Our primarily data showed that mutant CCL21 in xenograft brain tumor models showed substantial inhibition of tumour growth. Our results indicate that truncated CCL21 chemokine might be a potential preventive biofactor for human breast cancer metastasis by targeting chemokine receptor genes.
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Yang, Kuan, Xiaxin Cao, Changqi Yan, Yongyong Yang, Chunping Tian y Jianjun Xu. "Visualization Study of Bubble Sliding Characteristics in a Subcooled Flow Boiling Narrow Rectangular Channel Under Natural Circulation Condition". En 2017 25th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/icone25-66843.

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Heat transfer enhancement by the motion of the bubbles sliding along the heating surface are wildly reported by many researchers, thus it is of great importance to quantitatively investigate the characteristics of sliding bubbles. A visualization study of subcooled flow boiling of water in a vertical single face-heated narrow rectangular channel under a series of natural circulation working conditions was conducted. Pictures of the bubble sliding behaviors were captured by a high speed camera simultaneously with thermal data. A sequence of digital image processing algorithms were applied to the original picture to extract bubble shape and location information, which post-processing methods were adopted to obtain characteristic sliding parameters (including the distribution of the equivalent sliding bubble diameter and velocity, number density of the sliding bubbles). It is found that bubbles can be able to nucleate and grow while sliding on the heating plate after the ONB point; the bubble number density, average bubble sliding velocity and the average sliding diameter continue to increase along the test section; heat transfer in the flow channel are significantly enhanced along flow direction with relatively low local void fraction. The average bubble sliding velocity near the inlet is significantly smaller than the sectional average velocity of single-phase fluid of the flow channel, and then it exceeded near the outlet of the test section. The average bubble sliding velocity and diameter increase with increasing heat flux and decreeing local subcooling degree. The equivalent diameter of sliding bubbles and the bubble sliding velocity approximately follow normal distribution. The distribution of the bubble diameter and velocity both cover a wild range. The standard deviations of the probability density function of the sliding bubble diameter and velocity increase with increasing heat flux and decreasing subcooling degree.
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Malki, Mohammed Imad. "Contribution of Glycosaminoglycan binding in CCL21-mediated Migration of Breast Cancer cells". En Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0081.

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Lymph node metastasis constitutes a key event in Breast Cancer progression and it is a process at least partially mediated by the chemokine CCR7. Chemokine function is dependent upon their binding to both cell-surface glycosaminoglycans (GAGs) molecules and to their specific receptors; thus, the role of GAGs in CCR7-mediated lymph node metastasis was investigated by creating a non-GAG binding chemokine CCL21 (mut-CCL21). Mut-CCL21 (Δ98-134) was synthesized, at 50nM it had similar potential to mobilize intracellular calcium compared to wild-type CCL21 (WT-CCL21). Next, a series of experiments was performed to determine how deletion of the GAG-binding site altered the ability of CCL21 to stimulate chemotaxis within a concentration gradient generated by free solute diffusion. Both WT-CCL21 and mut-CCL21 had a similar potential to stimulate chemotactic migration of PBMC (P>0.05). However, 4T1-Luc cells exhibit reduced migration at 30 & 50nM (p<0.001). Interestingly, this effect was greatly exacerbated in trans-endothelial migration, with the mut-CCL21 failing to increase cell migration above the background level at 30 nM in PBMC and 4T1-Luc cells (p> 0.001 vs WT). This difference could potentially be attributed to reduced GAG binding, as surface plasmon resonance spectroscopy showed that mut-CCL21 did not significantly bind heparan sulphate compared to the WT-CCL21. Finally, a murine model was used to assess the potential of mut-CCL21 to prevent lymph node metastasis in vivo. Mice were injected with 4T1-Luc cells in the mammary fat pad and treated daily for a week with 20µg mut-CCL21 once the tumor was visible. Mice were imaged weekly with IVIS to assess bioluminescence and sacrificed on day 18. Luciferase expression was significantly reduced in lymph nodes from mice that had been treated with mut-CCL21 compared to the control (p=0.0148), suggesting the potential to target chemokine binding to GAGs as a therapeutic option.
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Informes sobre el tema "The Wild Wynchesters series"

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Zamir, Dani y Steven Tanksley. Fine Mapping and Genetic Interactions of Nearly-Isogenic Allelic Series Representing Yield and Quality QTLs Derived from Wild Tomato Species. United States Department of Agriculture, julio de 2002. http://dx.doi.org/10.32747/2002.7586460.bard.

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Wild germplasm represents a rich source of QTLs capable of enhancing productivity of crop plants. Using the molecular linkage map of tomato in conjunction with novel population structures, we have identified QTLs from five Lycopersicon species that improve key yield and quality associated traits of processing tomatoes. In this research we employed multi-testing sites for fine mapping analysis of the different components of the affected traits combined with genetic interaction studies. Our results demonstrate that 'exotic libraries', which comprise of marker-defined genomic regions taken from wild species and introgressed onto the background of elite crop lines, provide an important opportunity for improving of the agricultural performance of modem crop varieties. Furthermore, we showed that these genetic resources can also serve as reagents for the discovery and characterization of genes that underlie traits of agricultural value. The results set the stage for using the QTLs in marker assisted programs and for applying map-based cloning of the targeted QTL/genes. The cloning of QTLs revealed genes that control pathways for agricultural yield in tomato that may be common for other crop species.
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2

Abbo, Shahal, Hongbin Zhang, Clarice Coyne, Amir Sherman, Dan Shtienberg y George J. Vandemark. Winter chickpea; towards a new winter pulse for the semiarid Pacific Northwest and wider adaptation in the Mediterranean basin. United States Department of Agriculture, enero de 2011. http://dx.doi.org/10.32747/2011.7597909.bard.

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Original objectives: [a] Screen an array of chickpea and wild annual Cicer germplasm for winter survival. [b] Genetic analysis of winter hardiness in domesticated x wild chickpea crosses. [c] Genetic analysis of vernalization response in domesticated x wild chickpea crosses. [d] Digital expression analysis of a core selection of breeding and germplasm lines of chickpea that differ in winter hardiness and vernalization. [e] Identification of the genes involved in the chickpea winter hardiness and vernalization and construction of gene network controlling these traits. [f] Assessing the phenotypic and genetic correlations between winter hardiness, vernalization response and Ascochyta blight response in chickpea. The complexity of the vernalization response and the inefficiency of our selection experiments (below) required quitting the work on ascochyta response in the framework of this project. Background to the subject: Since its introduction to the Palouse region of WA and Idaho, and the northern Great Plains, chickpea has been a spring rotation legume due to lack of winter hardiness. The short growing season of spring chickpea limits its grain yield and leaves relatively little stubble residue for combating soil erosion. In Israel, chilling temperatures limit pod setting in early springs and narrow the effective reproductive time window of the crop. Winter hardiness and vernalization response of chickpea alleles were lost due to a series of evolutionary bottlenecks; however, such alleles are prevalent in its wild progenitor’s genepool. Major conclusions, solutions, achievements: It appears that both vernalization response and winter hardiness are polygenic traits in the wild-domesticated chickpea genepool. The main conclusion from the fieldwork in Israel is that selection of domesticated winter hardy and vernalization responsive types should be conducted in late flowering and late maturity backgrounds to minimize interference by daylength and temperature response alleles (see our Plant Breeding paper on the subject). The main conclusion from the US winter-hardiness studies is that excellent lines have been identified for germplasm release and continued genetic study. Several of the lines have good seed size and growth habit that will be useful for introgressing winter-hardiness into current chickpea cultivars to develop releases for autumn sowing. We sequenced the transcriptomes and profiled the expression of genes in 87 samples. Differential expression analysis identified a total of 2,452 differentially expressed genes (DEGs) between vernalized plants and control plants, of which 287 were shared between two or more Cicer species studied. We cloned 498 genes controlling vernalization, named CVRN genes. Each of the CVRN genes contributes to flowering date advance (FDA) by 3.85% - 10.71%, but 413 (83%) other genes had negative effects on FDA, while only 83 (17%) had positive effects on FDA, when the plant is exposed to cold temperature. The cloned CVRN genes provide new toolkits and knowledge to develop chickpea cultivars that are suitable for autumn-sowing. Scientific & agricultural implications: Unlike the winter cereals (barley, wheat) or pea, in which a single allelic change may induce a switch from winter to spring habit, we were unable to find any evidence for such major gene action in chickpea. In agricultural terms this means that an alternative strategy must be employed in order to isolate late flowering – ascochyta resistant (winter types) domesticated forms to enable autumn sowing of chickpea in the US Great Plains. An environment was identified in U.S. (eastern Washington) where autumn-sown chickpea production is possible using the levels of winter-hardiness discovered once backcrossed into advanced cultivated material with acceptable agronomic traits. The cloned CVRN genes and identified gene networks significantly advance our understanding of molecular mechanisms underlying plant vernalization in general, and chickpea in particular, and provide a new toolkit for switching chickpea from a spring-sowing to autumn-sowing crop.
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3

Mwebe, Robert, Chester Kalinda, Ekwaro A. Obuku, Eve Namisango, Alison A. Kinengyere, Moses Ocan, Ann Nanteza, Savino Biryomumaisho y Lawrence Mugisha. Epidemiology and effectiveness of interventions for Foot and Mouth Disease in Africa: A protocol for systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, noviembre de 2022. http://dx.doi.org/10.37766/inplasy2022.11.0039.

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Review question / Objective: What is the epidemiology and effectiveness of control measures for foot and mouth disease in African countries?’ PICOS: Description of elements Population/ problem/Setting: Artiodactyla (cloven ungulates), domestic (cattle, sheep, goats, and pigs), camels and wildlife (buffaloes, deer, antelope, wild pigs, elephant, giraffe, and camelids) affected by Foot and Mouth Disease (FMD) or Hoof and Mouth Disease (HMD) caused by the Foot and Mouth Disease Virus (FMDV) in Africa. Intervention: Prevention measures: vaccination, ‘biosafety and biosecurity’, sensitization of the public. Control measures: quarantine, movement control, closure of markets and stock routes, mouth swabbing of animals with infected materials (old technique that is no long applicable), culling, mass slaughter, stamping out and any other interventions or control measures generally accepted by the ‘community of practice’ of animal health practitioners. Comparator: areas that did not have any control activities for FMD, in head-to-head comparisons in the same study. Outcome: epidemiological outcomes: incidence, prevalence, patterns or trends, clinical symptoms, and risk factors. Effectiveness outcomes: success, and usefulness of the interventions measured as averted deaths, illness and infections, and costs associated with the interventions (cost–effectiveness). Study design: epidemiological designs include cohort design for incidence, cross sectional for prevalence and case-control for clinical symptoms and risk factors. Interventional designs include randomized controlled trials, cluster randomized trials, quasi-experimental designs – controlled before and after, interrupted time series, [regression discontinuity design, difference-in-difference, and propensity score matching]. Timelines: 1900 – 2022.
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4

Ohad, Itzhak y Himadri Pakrasi. Role of Cytochrome B559 in Photoinhibition. United States Department of Agriculture, diciembre de 1995. http://dx.doi.org/10.32747/1995.7613031.bard.

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The aim of this research project was to obtain information on the role of the cytochrome b559 in the function of Photosystem-II (PSII) with special emphasis on the light induced photo inactivation of PSII and turnover of the photochemical reaction center II protein subunit RCII-D1. The major goals of this project were: 1) Isolation and sequencing of the Chlamydomonas chloroplast psbE and psbF genes encoding the cytochrome b559 a and b subunits respectively; 2) Generation of site directed mutants and testing the effect of such mutation on the function of PSII under various light conditions; 3) To obtain further information on the mechanism of the light induced degradation and replacement of the PSII core proteins. This information shall serve as a basis for the understanding of the role of the cytochrome b559 in the process of photoinhibition and recovery of photosynthetic activity as well as during low light induced turnover of the D1 protein. Unlike in other organisms in which the psbE and psbF genes encoding the a and b subunits of cytochrome b559, are part of an operon which also includes the psbL and psbJ genes, in Chlamydomonas these genes are transcribed from different regions of the chloroplast chromosome. The charge distribution of the derived amino-acid sequences of psbE and psbF gene products differs from that of the corresponding genes in other organisms as far as the rule of "positive charge in" is concerned relative to the process of the polypeptide insertion in the thylakoid membrane. However, the sum of the charges of both subunits corresponds to the above rule possibly indicating co-insertion of both subunits in the process of cytochrome b559 assembly. A plasmid designed for the introduction of site-specific mutations into the psbF gene of C. reinhardtii. was constructed. The vector consists of a DNA fragment from the chromosome of C. reinhardtii which spans the region of the psbF gene, upstream of which the spectinomycin-resistance-conferring aadA cassette was inserted. This vector was successfully used to transform wild type C. reinhardtii cells. The spectinomycin resistant strain thus obtained can grow autotrophically and does not show significant changes as compared to the wild-type strain in PSII activity. The following mutations have been introduced in the psbF gene: H23M; H23Y; W19L and W19. The replacement of H23 involved in the heme binding to M and Y was meant to permit heme binding but eventually alter some or all of the electron transport properties of the mutated cytochrome. Tryptophane W19, a strictly conserved residue, is proximal to the heme and may interact with the tetrapyrole ring. Therefore its replacement may effect the heme properties. A change to tyrosine may have a lesser affect on the potential or electron transfer rate while a replacement of W19 by leucine is meant to introduce a more prominent disturbance in these parameters. Two of the mutants, FW19L and FH23M have segregated already and are homoplasmic. The rest are still grown under selection conditions until complete segregation will be obtained. All mutants contain assembled and functional PSII exhibiting an increased sensitivity of PSII to the light. Work is still in progress for the detailed characterization of the mutants PSII properties. A tobacco mutant, S6, obtained by Maliga and coworkers harboring the F26S mutation in the b subunit was made available to us and was characterized. Measurements of PSII charge separation and recombination, polypeptide content and electron flow indicates that this mutation indeed results in light sensitivity. Presently further work is in progress in the detailed characterization of the properties of all the above mutants. Information was obtained demonstrating that photoinactivation of PSII in vivo initiates a series of progressive changes in the properties of RCII which result in an irreversible modification of the RCII-D1 protein leading to its degradation and replacement. The cleavage process of the modified RCII-D1 protein is regulated by the occupancy of the QB site of RCII by plastoquinone. Newly synthesized D1 protein is not accumulated in a stable form unless integrated in reassembled RCII. Thus the degradation of the irreversibly modified RCII-D1 protein is essential for the recovery process. The light induced degradation of the RCII-D1 protein is rapid in mutants lacking the pD1 processing protease such as in the LF-1 mutant of the unicellular alga Scenedesmus obliquus. In this case the Mn binding site of PSII is abolished, the water oxidation process is inhibited and harmful cation radicals are formed following light induced electron flow in PSII. In such mutants photo-inactivation of PSII is rapid, it is not protected by ligands binding at the QB site and the degradation of the inactivated RCII-D1 occurs rapidly also in the dark. Furthermore the degraded D1 protein can be replaced in the dark in absence of light driven redox controlled reactions. The replacement of the RCII-D1 protein involves the de novo synthesis of the precursor protein, pD1, and its processing at the C-terminus end by an unknown processing protease. In the frame of this work, a gene previously isolated and sequenced by Dr. Pakrasi's group has been identified as encoding the RCII-pD1 C-terminus processing protease in the cyanobacterium Synechocystis sp. PCC 6803. The deduced sequence of the ctpA protein shows significant similarity to the bovine, human and insect interphotoreceptor retinoid-binding proteins. Results obtained using C. reinhardtii cells exposes to low light or series of single turnover light flashes have been also obtained indicating that the process of RCII-D1 protein turnover under non-photoinactivating conditions (low light) may be related to charge recombination in RCII due to back electron flow from the semiquinone QB- to the oxidised S2,3 states of the Mn cluster involved in the water oxidation process.
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5

Wisniewski, Michael, Samir Droby, John Norelli, Dov Prusky y Vera Hershkovitz. Genetic and transcriptomic analysis of postharvest decay resistance in Malus sieversii and the identification of pathogenicity effectors in Penicillium expansum. United States Department of Agriculture, enero de 2012. http://dx.doi.org/10.32747/2012.7597928.bard.

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Use of Lqh2 mutants (produced at TAU) and rNav1.2a mutants (produced at the US side) for identifying receptor site-3: Based on the fact that binding of scorpion alpha-toxins is voltage-dependent, which suggests toxin binding at the mobile voltage-sensing region, we analyzed which of the toxin bioactive domains (Core-domain or NC-domain) interacts with the DIV Gating-module of rNav1.2a. This analysis was based on the assumption that the dissociation of toxin mutants upon depolarization would vary from that of the unmodified toxin should the substitutions affect a site of interaction with the channel Gating-module. Using a series of toxin mutants (mutations at both domains) and two channel mutants that were shown to reduce the sensitivity to scorpion alpha-toxins, and by comparison of depolarization-driven dissociation of Lqh2 derivatives off their binding site at rNav1.2a mutant channels we found that the toxin Core-domain interacts with the Gating-module of DIV. Details of the experiments and results appear in Guret al (2011). Mapping receptor site 3 at Nav1.2a by extensive channel mutagenesis (Seattle): Since previous studies with photoaffinity labeling and antibody mapping implicated domains I and IV in scorpion alpha-toxin binding, Nav1.2 channel mutants containing substitutions at these extracellular regions were expressed and tested for receptor function by whole-cell voltage clamp. Of a large number of channel mutants, T1560A, F1610A, and E1613A in domain IV had ~5.9-, ~10.7-, and ~3.9-fold lower affinities for the scorpion toxin Lqh2, respectively, and mutant E1613R had 73-fold lower affinity. Toxin dissociation was accelerated by depolarization for both wild-type and mutants, and the rates of dissociation were also increased by mutations T1560A, F1610A and E1613A. In contrast, association rates for these three mutant channels at negative membrane potentials were not significantly changed and were not voltage-dependent. These results indicated that Thr1560 in the S1-S2 loop, Phe1610 in the S3 segment, and Glu1613 in the S3-S4 loop in domain IV participate in toxin binding. T393A in the SS2-S6 loop in domain I also showed a ~3.4-fold lower affinity for Lqh2, indicating that this extracellular loop may form a secondary component of the toxin binding site. Analysis with the Rosetta-Membrane algorithm revealed a three-dimensional model of Lqh2 binding to the voltage sensor in a resting state. In this model, amino acid residues in an extracellular cleft formed by the S1-S2 and S3-S4 loops in domain IV that are important for toxin binding interact with amino acid residues on two faces of the wedge-shaped Lqh2 molecule that are important for toxin action. The conserved gating charges in the S4 transmembrane segment are in an inward position and likely form ion pairs with negatively charged amino acid residues in the S2 and S3 segments (Wang et al 2011; Gurevitz 2012; Gurevitzet al 2013).
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6

Ziesler, Pamela y Claire Spalding. Statistical abstract: 2021. National Park Service, mayo de 2022. http://dx.doi.org/10.36967/nrds-2293345.

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In 2021, recreation visits to National Park Service (NPS) sites rebounded from the COVID-19 pandemic-driven low visitation of 2020 and climbed to 297,115,406 recreation visits. This is an increase of 60 million recreation visits (+25.3%) from 2020 and a decrease of 30 million recreation visits (-9.3%) from 2019. Recreation visitor hours were 1,356,657,749 – a 28.6% increase from 2020 and a 5.1% decrease from 2019. Total overnight stays followed a similar pattern with 12,745,455 overnight stays – up 4.7 million (+58.5%) from 2020 and down 1.1 million (-8%) from 2019. Five parks were added to the reporting system in 2021: Alagnak Wild River in Alaska, Camp Nelson National Monument in Kentucky, Medgar and Myrlie Evers Home National Monument in Mississippi, Tule Springs Fossil Beds National Monument in Nevada, and World War I Memorial in Washington, D.C. These parks were responsible for over 629,000 recreation visits in 2021. Factors influencing visits to National Park System units in 2021 include: continuing closures and limited capacities due to COVID-19 mitigation at some parks, temporary closures for wildland fires in 2021 (eleven parks), severe regional smoke/haze from ongoing wildland fires throughout the summer and early autumn affecting parks in the western half and northern tier of states in the continental U.S., two hurricanes in 2021 – both in August – impacted visitation: Hurricane Henri caused temporary closures of some parks in the northeast and Hurricane Ida caused temporary closures of parks along the Gulf Coast and generated some heavy flooding in the northeast, hurricanes and wildland fires in previous years resulting in lingering closures, most notably Hurricanes Irma and Maria in 2017, the Carr and Woolsey Fires in 2018, Hurricane Dorian in 2019, the Caldwell, Cameron Peak, East Troublesome, and Woodward Fires in 2020, and Hurricane Sally in 2020. Forty-four parks set a record for recreation visits in 2021 and 6 parks broke a record they set in 2020. See Appendix A for a list of record parks. The number of reporting units with over 10 million recreation visits was the same as in recent years (3 parks) and 73 parks had over 1 million recreation visits. Twenty-five percent of total recreation visits occurred in the top 8 parks and fifty percent of total visitation occurred in the top 25 parks. Several parks passed annual visitation milestones including Capulin Volcano NM which passed 100,000 annual recreation visits for the first time, Big Bend NP and Devils Tower NM which each passed 500,000 annual recreation visits for the first time, and Zion NP which passed 5 million visits for the first time. Other parks passed milestones for accumulated recreation visits including Hamilton Grange NMEM (1968-2021) and Palo Alto Battlefield NHP (2003-2021) each passing 1 million total recreation visits, Voyageurs NP (1976-2021) passing 10 million total recreation visits, and Hot Springs NP (1904-2021) passing 100 million total recreation visits. Population center designations were updated in 2021 to reflect overlap of park boundaries with statistical areas from the 2020 U.S. Census. Many population center changes reflect increases in local population as indicated by parks changing from rural to outlying or from outlying to suburban. Other changes reflect increasing complexity in population density as parks changed from a single designation, such as rural or suburban, to a mixed designation. See the Definitions section for population center definitions and Table B.1 for previous and updated population center designations by park. In the pages that follow, a series of tables and figures display visitor use data for calendar year 2021. By documenting these visits across the National Park System, the NPS Statistical Abstract offers a historical record of visitor use in parks and provides NPS staff and partners with a useful tool for effective management and planning. In 2021, 394 of 423 NPS units...
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7

Epel, Bernard y Roger Beachy. Mechanisms of intra- and intercellular targeting and movement of tobacco mosaic virus. United States Department of Agriculture, noviembre de 2005. http://dx.doi.org/10.32747/2005.7695874.bard.

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To cause disease, plant viruses must replicate and spread locally and systemically within the host. Cell-to-cell virus spread is mediated by virus-encoded movement proteins (MPs), which modify the structure and function of plasmodesmata (Pd), trans-wall co-axial membranous tunnels that interconnect the cytoplasm of neighboring cells. Tobacco mosaic virus (TMV) employ a single MP for cell- cell spread and for which CP is not required. The PIs, Beachy (USA) and Epel (Israel) and co-workers, developed new tools and approaches for study of the mechanism of spread of TMV that lead to a partial identification and molecular characterization of the cellular machinery involved in the trafficking process. Original research objectives: Based on our data and those of others, we proposed a working model of plant viral spread. Our model stated that MPᵀᴹⱽ, an integral ER membrane protein with its C-terminus exposed to the cytoplasm (Reichel and Beachy, 1998), alters the Pd SEL, causes the Pd cytoplasmic annulus to dilate (Wolf et al., 1989), allowing ER to glide through Pd and that this gliding is cytoskeleton mediated. The model claimed that in absence of MP, the ER in Pd (the desmotubule) is stationary, i.e. does not move through the Pd. Based on this model we designed a series of experiments to test the following questions: -Does MP potentiate ER movement through the Pd? - In the presence of MP, is there communication between adjacent cells via ER lumen? -Does MP potentiate the movement of cytoskeletal elements cell to cell? -Is MP required for cell-to-cell movement of ER membranes between cells in sink tissue? -Is the binding in situ of MP to RNA specific to vRNA sequences or is it nonspecific as measured in vitro? And if specific: -What sequences of RNA are involved in binding to MP? And finally, what host proteins are associated with MP during intracellular targeting to various subcellular targets and what if any post-translational modifications occur to MP, other than phosphorylation (Kawakami et al., 1999)? Major conclusions, solutions and achievements. A new quantitative tool was developed to measure the "coefficient of conductivity" of Pd to cytoplasmic soluble proteins. Employing this tool, we measured changes in Pd conductivity in epidermal cells of sink and source leaves of wild-type and transgenic Nicotiana benthamiana (N. benthamiana) plants expressing MPᵀᴹⱽ incubated both in dark and light and at 16 and 25 ᵒC (Liarzi and Epel, 2005 (appendix 1). To test our model we measured the effect of the presence of MP on cell-to-cell spread of a cytoplasmic fluorescent probe, of two ER intrinsic membrane protein-probes and two ER lumen protein-probes fused to GFP. The effect of a mutant virus that is incapable of cell-to-cell spread on the spread of these probes was also determined. Our data shows that MP reduces SEL for cytoplasmic molecules, dilates the desmotubule allowing cell-cell diffusion of proteins via the desmotubule lumen and reduces the rate of spread of the ER membrane probes. Replicase was shown to enhance cell-cell spread. The data are not in support of the proposed model and have led us to propose a new model for virus cell-cell spread: this model proposes that MP, an integral ER membrane protein, forms a MP:vRNAER complex and that this ER-membrane complex diffuses in the lipid milieu of the ER into the desmotubule (the ER within the Pd), and spreads cell to cell by simple diffusion in the ER/desmotubule membrane; the driving force for spread is the chemical potential gradient between an infected cell and contingent non-infected neighbors. Our data also suggests that the virus replicase has a function in altering the Pd conductivity. Transgenic plant lines that express the MP gene of the Cg tobamovirus fused to YFP under the control the ecdysone receptor and methoxyfenocide ligand were generated by the Beachy group and the expression pattern and the timing and targeting patterns were determined. A vector expressing this MPs was also developed for use by the Epel lab . The transgenic lines are being used to identify and isolate host genes that are required for cell-to-cell movement of TMV/tobamoviruses. This line is now being grown and to be employed in proteomic studies which will commence November 2005. T-DNA insertion mutagenesis is being developed to identify and isolate host genes required for cell-to-cell movement of TMV.
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