Artículos de revistas: "Terpene-isolation"

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Lai, Shih-Ming, I.-Wen Chen y Ming-Jyi Tsai. "Preparative isolation of terpene trilactones from Ginkgo biloba leaves". Journal of Chromatography A 1092, n.º 1 (octubre de 2005): 125–34. http://dx.doi.org/10.1016/j.chroma.2005.01.028.

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Yang, Chang-Qing, Xiu-Ming Wu, Ju-Xin Ruan, Wen-Li Hu, Yin-Bo Mao, Xiao-Ya Chen y Ling-Jian Wang. "Isolation and characterization of terpene synthases in cotton (Gossypium hirsutum)". Phytochemistry 96 (diciembre de 2013): 46–56. http://dx.doi.org/10.1016/j.phytochem.2013.09.009.

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Coleman, W. M., Bert M. Gordon y Brian M. Lawrence. "Examinations of the Matrix Isolation Fourier Transform Infrared Spectra of Organic Compounds: Part XII". Applied Spectroscopy 43, n.º 2 (febrero de 1989): 298–304. http://dx.doi.org/10.1366/0003702894203273.

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Matrix isolation Fourier transform infrared spectra (MI/FT-IR), mass spectra (MS), carbon-13 Nuclear Magnetic Resonance (13C-NMR) spectra, condensed-phase infrared spectra, and vapor-phase infrared (IR) spectra are presented for a series of terpene compounds. Subtle differences in positional and configurational isomers commonly found with terpenes could be easily detected by the MI/FT-IR spectra. The results are comparable in some aspects to those obtainable from 13C-NMR and thin-film IR; however, most importantly, they are acquired at the low nanogram level for MI/FT-IR, as compared to the milligram level for the other techniques. These results represent an advance in the technology available for the analysis of complex mixtures such as essential oils containing terpene-like molecules.

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4

Trapp, Susan C. y Rodney B. Croteau. "Genomic Organization of Plant Terpene Synthases and Molecular Evolutionary Implications". Genetics 158, n.º 2 (1 de junio de 2001): 811–32. http://dx.doi.org/10.1093/genetics/158.2.811.

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Abstract Terpenoids are the largest, most diverse class of plant natural products and they play numerous functional roles in primary metabolism and in ecological interactions. The first committed step in the formation of the various terpenoid classes is the transformation of the prenyl diphosphate precursors, geranyl diphosphate, farnesyl diphosphate, and geranylgeranyl diphosphate, to the parent structures of each type catalyzed by the respective monoterpene (C10), sesquiterpene (C15), and diterpene synthases (C20). Over 30 cDNAs encoding plant terpenoid synthases involved in primary and secondary metabolism have been cloned and characterized. Here we describe the isolation and analysis of six genomic clones encoding terpene synthases of conifers, [(-)-pinene (C10), (-)-limonene (C10), (E)-α-bisabolene (C15), δ-selinene (C15), and abietadiene synthase (C20) from Abies grandis and taxadiene synthase (C20) from Taxus brevifolia], all of which are involved in natural products biosynthesis. Genome organization (intron number, size, placement and phase, and exon size) of these gymnosperm terpene synthases was compared to eight previously characterized angiosperm terpene synthase genes and to six putative terpene synthase genomic sequences from Arabidopsis thaliana. Three distinct classes of terpene synthase genes were discerned, from which assumed patterns of sequential intron loss and the loss of an unusual internal sequence element suggest that the ancestral terpenoid synthase gene resembled a contemporary conifer diterpene synthase gene in containing at least 12 introns and 13 exons of conserved size. A model presented for the evolutionary history of plant terpene synthases suggests that this superfamily of genes responsible for natural products biosynthesis derived from terpene synthase genes involved in primary metabolism by duplication and divergence in structural and functional specialization. This novel molecular evolutionary approach focused on genes of secondary metabolism may have broad implications for the origins of natural products and for plant phylogenetics in general.

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Awakawa, Takayoshi. "Enzymatic reactions in teleocidin B biosynthesis". Journal of Natural Medicines 75, n.º 3 (6 de marzo de 2021): 467–74. http://dx.doi.org/10.1007/s11418-021-01504-2.

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AbstractThe teleocidin B family members are terpene indole compounds isolated from Streptomyces bacteria, and they strongly activate protein kinase C (PKC). Their unique structures have attracted many researchers in the natural product chemistry and pharmacology fields, and numerous isolation and bioactivity studies have been conducted. The accumulated information has facilitated the identification of the enzymatic reactions in teleocidin biosynthesis, and new developments in structural biology have strongly aided efforts to clarify the finer points of these reactions. This review describes the recent biochemical and structural biological studies to reveal their reaction mechanisms, with a primary focus on the terpene cyclization triggered by the C-N bond formation by P450 oxygenase (TleB), the prenyltransferase (TleC), and the methyltransferase (TleD). This new knowledge will benefit future engineering studies to create unnatural PKC activators.

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Weiss, Eve R., Jana Pika y Robert J. Braddock. "Isolation and Identification of Terpene Chlorohydrins Found in Cold-Pressed Orange Oil". Journal of Agricultural and Food Chemistry 51, n.º 8 (abril de 2003): 2277–82. http://dx.doi.org/10.1021/jf026122n.

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Wang, Piao-Yi, Rong Ni, Ting-Ting Zhu, Chun-Jing Sun, Hong-Xiang Lou, Xuebin Zhang y Ai-Xia Cheng. "Isolation and functional characterization of four microbial type terpene synthases from ferns". Plant Physiology and Biochemistry 155 (octubre de 2020): 716–24. http://dx.doi.org/10.1016/j.plaphy.2020.08.037.

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Hattan, Jun-ichiro, Kazutoshi Shindo, Tetsuya Sasaki y Norihiko Misawa. "Isolation and Functional Characterization of New Terpene Synthase Genes from Traditional Edible Plants". Journal of Oleo Science 67, n.º 10 (2018): 1235–46. http://dx.doi.org/10.5650/jos.ess18163.

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9

Jasiem, Thamer Mouhi. "Isolation of terpene and pharmacognostical study of iraqi caper shrubs (Capparis spinosa) L." Research Journal of Pharmacy and Technology 11, n.º 6 (2018): 2388. http://dx.doi.org/10.5958/0974-360x.2018.00441.9.

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10

French, Larry G. "Isolation and Structure Elucidation of the Terpene β-Thujone from Cedar Leaf Oil". Journal of Chemical Education 88, n.º 6 (junio de 2011): 829–31. http://dx.doi.org/10.1021/ed100620b.

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Weyerstahl, Peter, Helga Marschall-Weyerstahl, Martin Schröder y Vijay K. Kaul. "Terpenes and terpene derivatives, XXIV. Isolation and stereochemistry of the four artedouglasia oxides". Liebigs Annalen der Chemie 1988, n.º 9 (14 de septiembre de 1988): 917–18. http://dx.doi.org/10.1002/jlac.198819880919.

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de Mesquita, Mariana L., José E. de Paula, Laila S. Espindola, Luiz A. L. Soares, Tania M. G. da Silva, Celso A. Camara y Telma M. G. Da Silva. "Protoflavanones from the Wood Stem of Salvertia convallariodora". Natural Product Communications 12, n.º 4 (abril de 2017): 1934578X1701200. http://dx.doi.org/10.1177/1934578x1701200413.

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Phytochemical analysis of the ethyl acetate extract of stem wood of Salvertia convallariodora A. St.-Hil. (Vochysiaceae), a Brazilian Cerrado species, led to the isolation and full characterization of three new non-aromatic B-ring flavanones (1-3) as well as the terpene mixture of sericic acid (4), 24-hydroxytormentic acid (5), 24-hydroxytormentic acid glucosyl ester (6), and sericoside (7), all identified for the first time from S. convallariodora. The structures of the new flavanones (1-3) were established from IR, LC-PDA-qTOF-MS, and NMR spectral data, including 2D NMR experiments.

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13

Atolani, Olubunmi y Gabriel A. Olatunji. "Isolation and evaluation of antiglycation potential of polyalthic acid (furano-terpene) from Daniella oliveri". Journal of Pharmaceutical Analysis 4, n.º 6 (diciembre de 2014): 407–11. http://dx.doi.org/10.1016/j.jpha.2014.05.002.

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14

Okoth, Dorothy A., Joachim J. Hug, Ronald Garcia, Cathrin Spröer, Jörg Overmann y Rolf Müller. "2-Hydroxysorangiadenosine: Structure and Biosynthesis of a Myxobacterial Sesquiterpene–Nucleoside". Molecules 25, n.º 11 (9 de junio de 2020): 2676. http://dx.doi.org/10.3390/molecules25112676.

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Myxobacteria represent an under-investigated source for biologically active natural products featuring intriguing structural moieties with potential applications, e.g., in the pharmaceutical industry. Sorangiadenosine and the here-discovered 2-hydroxysorangiadenosine are myxobacterial sesquiterpene–nucleosides with an unusual structural moiety, a bicyclic eudesmane-type sesquiterpene. As the biosynthesis of these rare terpene–nucleoside hybrid natural products remains elusive, we investigated secondary metabolomes and genomes of several 2-hydroxysorangiadenosine-producing myxobacteria. We report the isolation and full structure elucidation of 2-hydroxysorangiadenosine and its cytotoxic and antibiotic activities and propose a biosynthetic pathway in the myxobacterium Vitiosangium cumulatum MCy10943T.

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15

Прокопчук (Prokopchuk), Денис (Denis) Игоревич (Igorevich), Олег (Oleg) Игоревич (Igorevich) Покровский (Pokrovskiy), Ольга (Ol'ga) Олеговна (Olegovna) Паренаго (Parenago), Саида (Saida) Амирановна (Аmiranovna) Багателия (Bagatelia), Алхас (Аlkhas) Анатольевич (Аnatol'evich) Марколия (Markolia), Сергей (Sergej) Александрович (Аleksandrovich) Покрышкин (Pokryshkin) y Валерий (Valerij) Васильевич (Vasil'evich) Лунин (Lunin). "COMPARISON OF THE QUALITATIVE COMPOSITION OF LAURUS NOBILIS LEAVES EXTRACTS OBTAINED BY SUPERCRITICAL FLUID EXTRACTION AND MICROWAVE-ASSISTED EXTRACTION". chemistry of plant raw material, n.º 3 (20 de marzo de 2018): 169–77. http://dx.doi.org/10.14258/jcprm.2018033758.

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Along with traditional methods for processing aroma plants, such as steam distillation or extraction with organic solvents, novel alternative approaches are developed which provide the means for a more effective isolation of biologically active compounds from plant matrices and simultaneously possess ecological attractiveness. Supercritical fluid extraction and microwave-assisted extraction can be attributed to such approaches. Their implementation into routine practice is partially hampered by somewhat vague understanding of applicability areas of these methods as well as by the lack of knowledge on the influence of process parameters on the result. In this work, we performed a comparison of the applicability of supercritical fluid and microwave-assisted extraction techniques for the isolation of biologically active compounds from leaves of Laurus nobilis L. It was shown that microwave-assisted extraction allows obtaining oil containing only volatile terpene components. Supercritical fluid extraction allows isolation of not only volatile terpenes from laurel leaves, but a broader spectrum of compounds including eugenol derivatives, phytosteroids and tocopherols. Qualitative composition of supercritical fluid extracts is virtually independent on process parameters, only quantitative differences in component proportions are registered.

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Kung, Chen, Chao, Wu, Lin y Chen. "Analysis of Volatile Constituents in Platostoma palustre (Blume) Using Headspace Solid-Phase Microextraction and Simultaneous Distillation-Extraction". Foods 8, n.º 9 (14 de septiembre de 2019): 415. http://dx.doi.org/10.3390/foods8090415.

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Hsian-tsao (Platostoma palustre Blume) is a traditional Taiwanese food. It is admired by many consumers, especially in summer, because of its aroma and taste. This study reports the analysis of the volatile components present in eight varieties of Hsian-tsao using headspace solid-phase microextraction (HS-SPME) and simultaneous distillation-extraction (SDE) coupled with gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). HS-SPME is a non-heating method, and the results show relatively true values of the samples during flavor isolation. However, it is a kind of headspace analysis that has the disadvantage of a lower detection ability to relatively higher molecular weight compounds; also, the data are not quantitative, but instead are used for comparison. The SDE method uses distillation 2 h for flavor isolation; therefore, it quantitatively identifies more volatile compounds in the samples while the samples withstand heating. Both methods were used in this study to investigate information about the samples. The results showed that Nongshi No. 1 had the highest total quantity of volatile components using HS-SPME, whereas SDE indicated that Taoyuan Mesona 1301 (TYM1301) had the highest volatile concentration. Using the two extraction methods, 120 volatile components were identified. Fifty-six volatile components were identified using HS-SPME, and the main volatile compounds were α-pinene, β-pinene, and limonene. A total of 108 volatile components were identified using SDE, and the main volatile compounds were α-bisabolol, β-caryophyllene, and caryophyllene oxide. Compared with SDE, HS-SPME sampling extracted a significantly higher amount of monoterpenes and had a poorer detection of less volatile compounds, such as sesquiterpenes, terpene alcohols, and terpene oxide.

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J. Fradi, Ahmed. "Evaluation the Effectiveness of Different Concentrations Phenols, Alkaloids and Terpenes Extracted from Pimpinella anisum against Phytophthora Fungi." Ibn AL- Haitham Journal For Pure and Applied Sciences 35, n.º 2 (20 de abril de 2022): 1–6. http://dx.doi.org/10.30526/35.2.2746.

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This study did the isolation, purification, and identification of the fungus Phytophthora cinnamomi of some infected plants, including Chili pepper, cucumber, and eggplant. The green parts of Pimpinella anisum plant were grounded to a semi-powdered state. Phenols, alkaloids and terpenes were extracted from this plant, then the anti-fungal activity was evaluated at different concentrations of 5% and 10%. The percentage of radial growth inhibition of fungi with plant extracts was measured after seven days of incubation. The results showed that the terpene extract was the most effective against fungi and the alkaloid extract had the least antifungal activity. the percentage of radial growth inhibition was 97%-65%, respectively.

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Garson, Mary J., Annette F. Dexter, Lynette K. Lambert y Vicky Liokas. "Isolation of the Bioactive Terpene 7-Deacetoxy-olepupuane from the Temperate Marine Spong Dysidea Sp." Journal of Natural Products 55, n.º 3 (marzo de 1992): 364–67. http://dx.doi.org/10.1021/np50081a013.

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19

Sulastri, Lilik, Ristanti Mega Lestari y Partomuan Simanjuntak. "Isolasi Dan Identifikasi Senyawa Kimia Monoterpen Dari Fraksi Etilasetat Daun Keji Beling (Strobilanthes crispa (L.) Blume) Yang Mempunyai Daya Sitotoksik". Jurnal Fitofarmaka Indonesia 8, n.º 1 (29 de enero de 2021): 12–17. http://dx.doi.org/10.33096/jffi.v8i1.721.

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Isolation and identification one of chemical compound mono-terpene from Keji beling plant (local name) (Strobilanthes crispa) has been done. Extraction was carried out by maceration of the keji beling leaf powder with ethanol 96%, then partitioned with water-ethyl acetate. Ethylacetate extract was isolated and purified by a cytotoxic guide fractionation system with Brine Shrimp Lethality Test (BSLT) on column chromatography (SiO2; n-hexane - ethylacetate = 20 : 1); and preparative Thin Layer Chromatography (TLC) (SiO2; n-hexane - ethylaseate = 10 : 1) which gave one compound in oily form. Based on identification by infra red spectra and gas chromatography-mass spectra (GC-MS), the compound is a monoterpene D-limone which has a cytotoxic power of 73,11 ppm.

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20

Katavic, Peter L., Pinus Jumaryatno, John N. A. Hooper, Joanne T. Blanchfield y Mary J. Garson. "Oxygenated Terpenoids from the Australian Sponges Coscinoderma matthewsi and Dysidea sp., and the Nudibranch Chromodoris albopunctata". Australian Journal of Chemistry 65, n.º 5 (2012): 531. http://dx.doi.org/10.1071/ch12010.

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The isolation and structure elucidation of seven new oxygenated terpenoids and eight known terpene metabolites from marine invertebrates collected at the Inner Gneerings Reef, South East Queensland, is discussed. Investigation of the sponge Coscinoderma matthewsi yielded an epoxylactone derivative (1) of the known furanoterpene tetradehydrofurospongin-1 (2). A chemical investigation of the dissected nudibranch Chromodoris albopunctata provided the new oxygenated diterpenes 12α-acetoxyspongian-16-one (10), 20-acetoxyspongian-16-one (12), 20-oxyspongian-16-one propionate (13) and 12α,20-dioxyspongian-16-one dipropionate (14) in conjunction with three other known diterpene metabolites, while two new chromodorolides, D (17) and E (18), in addition to four known diterpenes were isolated from a Dysidea sp.

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Katavic, Peter L., Pinus Jumaryatno, John N. A. Hooper, Joanne T. Blanchfield y Mary J. Garson. "Note of clarification about: Oxygenated Terpenoids from the Australian Sponges Coscinoderma matthewsi and Dysidea sp., and the Nudibranch Chromodoris albopunctata [vol. 65, pp. 531–538]". Australian Journal of Chemistry 66, n.º 11 (2013): 1461. http://dx.doi.org/10.1071/ch12010_nc.

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The isolation and structure elucidation of seven new oxygenated terpenoids and eight known terpene metabolites from marine invertebrates collected at the Inner Gneerings Reef, South East Queensland, is discussed. Investigation of the sponge Coscinoderma matthewsi yielded an epoxylactone derivative (1) of the known furanoterpene tetradehydrofurospongin-1 (2). A chemical investigation of the dissected nudibranch Chromodoris albopunctata provided the new oxygenated diterpenes 12?-acetoxyspongian-16-one (10), 20-acetoxyspongian-16-one (12), 20-oxyspongian-16-one propionate (13) and 12?,20-dioxyspongian-16-one dipropionate (14) in conjunction with three other known diterpene metabolites, while two new chromodorolides, D (17) and E (18), in addition to four known diterpenes were isolated from a Dysidea sp.

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Li, Huanhuan, Hongji Li, Shuo Chen, Wenhui Wu y Peng Sun. "Isolation and Identification of Pentalenolactone Analogs from Streptomyces sp. NRRL S-4". Molecules 26, n.º 23 (5 de diciembre de 2021): 7377. http://dx.doi.org/10.3390/molecules26237377.

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Terpene synthases are widely distributed in Actinobacteria. Genome sequencing of Streptomyces sp. NRRL S-4 uncovered a biosynthetic gene cluster (BGC) that putatively synthesizes pentalenolactone type terpenes. Guided by genomic information, the S-4 strain was chemically investigated, resulting in the isolation of two new sesquiterpenoids, 1-deoxy-8α-hydroxypentalenic acid (1) and 1-deoxy-9β-hydroxy-11-oxopentalenic acid (2), as shunt metabolites of the pentalenolactone (3) biosynthesis pathway. Their structures and absolute configurations were elucidated by analyses of HRESIMS and NMR spectroscopic data as well as time-dependent density functional theory/electronic circular dichroism (TDDFT/ECD) calculations. Compounds 1 and 2 exhibited moderate antimicrobial activities against Gram-positive and Gram-negative bacteria. These results confirmed that the pentalenolactone pathway was functional in this organism and will facilitate efforts for exploring Actinobacteria using further genome mining strategies.

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Yuping, Tang y Lou Fengchang. "SEPARATION AND ISOLATION OF TERPENE LACTONES FROM GINKGO BILOBA L. BY DIRECT HIGH PERFORMANCE LIQUID CHROMATOGRAPHY". Journal of Liquid Chromatography & Related Technologies 23, n.º 18 (enero de 2000): 2897–900. http://dx.doi.org/10.1081/jlc-100101241.

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Suciati, Lynette K. Lambert, Benjamin P. Ross, Myrna A. Deseo y Mary J. Garson. "Phytochemical Study of Fagraea spp. Uncovers a New Terpene Alkaloid with Anti-Inflammatory Properties". Australian Journal of Chemistry 64, n.º 4 (2011): 489. http://dx.doi.org/10.1071/ch10421.

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Phytochemical investigation of the stem bark of F. racemosa JACK ex WALL (Loganiaceae) from East Java, Indonesia, has resulted in the isolation of a new alkaloid fagraeoside along with the iridoid glycoside secologanoside. Fagraeoside may be derived from the condensation of secologanin with L-asparagine, and represents a rare example of a terpene alkaloid in which the amino acid component is non-aromatic. Investigation of three additional species of Fagraea provided known lignans, iridoid or secoiridoid glycosides, and flavanol-6-C-glucosides, thus it is likely that iridoid and secoiridoid glucosides are chemotaxonomic markers for the Fagraea genus. Fagraeoside inhibited the production of prostaglandin E2 in 3T3 murine fibroblasts (IC50 ~5.1 µM), and was not cytotoxic to this cell line or to a P388 murine leukaemia cell line. Selected isolated compounds, including fagraeoside, showed low to moderate activity in anti-acetylcholinesterase screening.

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Jørgensen, Lars, Steven J. McKerrall, Christian A. Kuttruff, Felix Ungeheuer, Jakob Felding y Phil S. Baran. "14-Step Synthesis of (+)-Ingenol from (+)-3-Carene". Science 341, n.º 6148 (1 de agosto de 2013): 878–82. http://dx.doi.org/10.1126/science.1241606.

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Ingenol is a diterpenoid with unique architecture and has derivatives possessing important anticancer activity, including the recently Food and Drug Administration–approved Picato, a first-in-class drug for the treatment of the precancerous skin condition actinic keratosis. Currently, that compound is sourced inefficiently from Euphorbia peplus. Here, we detail an efficient, highly stereocontrolled synthesis of (+)-ingenol proceeding in only 14 steps from inexpensive (+)-3-carene and using a two-phase design. This synthesis will allow for the creation of fully synthetic analogs of bioactive ingenanes to address pharmacological limitations and provides a strategic blueprint for chemical production. These results validate two-phase terpene total synthesis as not only an academic curiosity but also a viable alternative to isolation or bioengineering for the efficient preparation of polyoxygenated terpenoids at the limits of chemical complexity.

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Oladimeji, Olawale H. y Kufre E. Eberefiak. "Isolation and antimicrobial analysis of a steroidal terpene from the butanol fraction of Byrophyllum pinnatum (Lam.) Oken". European Chemical Bulletin 6, n.º 7 (12 de julio de 2017): 292. http://dx.doi.org/10.17628/ecb.2017.6.292-294.

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Vezzaro, Alice, Sandra T. Krause, Alberto Nonis, Angelo Ramina, Jörg Degenhardt y Benedetto Ruperti. "Isolation and characterization of terpene synthases potentially involved in flavor development of ripening olive (Olea europaea) fruits". Journal of Plant Physiology 169, n.º 9 (junio de 2012): 908–14. http://dx.doi.org/10.1016/j.jplph.2012.01.021.

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28

Nurhayati, Awik Puji Dyah, Rarastoeti Pratiwi, Subagus Wahyuono y Istriyati . "PROLIFERATION INHIBITORY ACTIVITY OF THE ACTIVE FRACTION MARINE SPONGE Cinachyrella sp. AGAINST CELL LINE T47D". KnE Life Sciences 2, n.º 1 (20 de septiembre de 2015): 663. http://dx.doi.org/10.18502/kls.v2i1.243.

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Marine sponges Cinachyrella sp. (Family:Tetillidae) in Kukup beach, Kemadang Village, Tanjungsari District, Gunung Kidul, DIY were producing diversity secondary metabolites such as polyketides, alkaloids, peptide and terpene. The purpose of this study was investigated proliferation inhibitory activity of active fraction Cinachyrella sp. against cell line T47D. Sponges samples were collected manually from rocky substrate at depth 0.5 m. The sponges was minced and extracted with 95% ethanol. The ethanol extract was partitioned sequentially with ethyl acetate. The extract ethyl acetate was fractionation with 4 organic solvent, in increasing order of polarity with vacuum liquid chromatography column (VLC) method. Doubling time method was applied to analyse the inhibition proliferative cell line T47D. Resulted showed ethyl acetate extract of Cinachyrella sp. were 12 fractions and all tested fraction obtained by thin layer chromatography (TLC). Fractions that have the same value Rf grouped together to obtain 6 fractions. The fraction number 5 exhibited proliferation inhibitory activity to cell line T47D. The Rf value of active fraction number 5 were 0.125; 0.25 and 0.437. The active fraction 5 than isolation by preparative thin-layer chromatography (PTLC) was 5 isolate fractions preparative. The isolate fractions preparative number 5 exhibited proliferation inhibitory activity against cell line T47D. Fraction which determined by cerium sulfate and results was expressed terpene and alkaloid. Keywords: Cinachyrella sp., Doubling time method, proliferation inhibitory activity.

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Jones, Christopher G., Christopher I. Keeling, Emilio L. Ghisalberti, Elizabeth L. Barbour, Julie A. Plummer y Jörg Bohlmann. "Isolation of cDNAs and functional characterisation of two multi-product terpene synthase enzymes from sandalwood, Santalum album L." Archives of Biochemistry and Biophysics 477, n.º 1 (septiembre de 2008): 121–30. http://dx.doi.org/10.1016/j.abb.2008.05.008.

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30

Guidoti, Daniela Granella Gomes, David Teixeira Guidoti, Adriano Lopes Romero, Ana Lúcia Tasca Gois Ruiz, Mary Ann Foglio, João Ernesto de Carvalho y Carmem Lúcia de Mello Sartori Cardoso da Rocha. "Kaurenoic acid from Annona squamosa L. exhibits antiproliferative effect on human tumor cell lines and induces apoptosis in Aspergillus nidulans". Revista Fitos 13, n.º 2 (10 de septiembre de 2019): 122–36. http://dx.doi.org/10.17648/2446-4775.2019.716.

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Annona squamosa is a source of bioactive compounds, with several pharmacological activities. However, tests are needed to confirm the safety of these compounds in terms of cytogenotoxic potential and their possible medicinal activity. Thus, the aims of the present study were to isolate kaurenoic acid from sugar apple peel and determine its antiproliferative activity in nine human tumor cell lines and mutagenic activity in germinating Aspergillus nidulans conidia. Chemical extraction resulted in the isolation of kaurenoic acid, a terpene that demonstrated a cytostatic effect at concentrations of 25 and 250 µg mL-1 and antiproliferative at a concentration 250 µg mL-1. The germination test showed that this compound can activate apoptosis, since there was an increase in dead conidia and a decrease in malformed individuals in all treatments. These results indicate its antiproliferative effect and apoptosis activation, representing a useful tool in the development of new chemotherapy drugs.

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El Midaoui, Adil, Farid Khallouki, Réjean Couture, Florina Moldovan, Mahmoud Ali Ismael, Brice Ongali, Marie Yvonne Akoume et al. "Thymus atlanticus: A Source of Nutrients with Numerous Health Benefits and Important Therapeutic Potential for Age-Related Diseases". Nutrients 15, n.º 18 (21 de septiembre de 2023): 4077. http://dx.doi.org/10.3390/nu15184077.

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Thymus atlanticus (Lamiaceae) is a plant endemic to the Mediterranean basin that is found in significant quantities in the arid regions of Morocco. Thymus atlanticus is used in traditional medicine to treat infectious and non-infectious diseases. It is also used for the isolation of essential oils and for the seasoning of many dishes in the Mediterranean diet. The major constituents of Thymus atlanticus are saponins, flavonoids, tannins, alkaloids, various simple and hydroxycinnamic phenolic compounds, and terpene compounds. Several of these compounds act on signaling pathways of oxidative stress, inflammation, and blood sugar, which are parameters often dysregulated during aging. Due to its physiochemical characteristics and biological activities, Thymus atlanticus could be used for the prevention and/or treatment of age-related diseases. These different aspects are treated in the present review, and we focused on phytochemistry and major age-related diseases: dyslipidemia, cardiovascular diseases, and type 2 diabetes.

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Mierza, Vriezka, Antolin Antolin, Audi Ichsani, Nurma Dwi, Sridevi Sridevi y Syfa Dwi. "Research Article: Isolasi dan Identifikasi Senyawa Terpenoid". Jurnal Surya Medika 9, n.º 2 (27 de agosto de 2023): 134–41. http://dx.doi.org/10.33084/jsm.v9i2.5681.

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Terpenoids are a group of hydrocarbons that are produced in large quantities by plants and are mainly contained in the sap and cell vacuoles. In plants, terpene compounds and their modifications, terpenoids, are secondary metabolites. Terpenoids make up many of the essential oils produced by plants. The content of essential oils influences the use of spice products, both as seasonings, as fragrances, as well as ingredients for medicine, health, etc. The terpenoid-steroid test was carried out using the Lieberman-Buchard reagent which yielded positive results for terpenoids for all fractions. The results obtained for the n-hexane and ethyl acetate fractions on the addition of lieberman-buchard yielded positive results for steroids as indicated by the formation of a green color in the fractions. The purpose of this research article is to provide information to find out what methods are used in isolation in identifying terpenoid compounds and knowing the types of terpenoid compounds obtained from the results of previous research journals.

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Schroeder, Daniel R. y Frank R. Stermitz. "Isolation and synthesis of bishordeninyl terpene alkaloids. Some experiments relating to the natural occurrence of formal Diels-Alder adducts". Tetrahedron 41, n.º 19 (enero de 1985): 4309–20. http://dx.doi.org/10.1016/s0040-4020(01)97202-3.

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Оленников (Olennikov), Даниил (Daniil) Николаевич (Nikolaevich) y Нина (Nina) Игоревна (Igorevna) Кащенко (Kashchenko). "PHYTOECDYSTEROIDS OF SERRATULA L. AND KLASEA CASS. GENERA: CHEMODIVERSITY, METHODS OF ISOLATION AND ANALYSIS". chemistry of plant raw material, n.º 4 (14 de septiembre de 2017): 123–35. http://dx.doi.org/10.14258/jcprm.2017042016.

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Serratula L. and Klasea Cass. are two systematically related genera of Asteraceae family containing phytoecdysteroids, a group of natural terpene compounds with various biological activity. Beginning from the 1970s, 76 phytoecdysteroids were isolated and identified in 13 species of Serratula and 5 species of Klasea. This review presented information on the chemodiversity of phytoecdysteroids of Serratula and Klasea genera and their occurrence in individual species. It was shown that the structural features of Serratula and Klasea phytoecdysteroids include the presence of a complete side chain at C-20 atom as well as 5 to 7 hydroxyl groups. Among the species studied, the most investigated were S. coronata, S. tinctoria and S. chinensis with 50, 21 and 19 known compounds, respectively. Also in the review the information on the methods of extraction, isolation and analysis of phytoecdysteroids of the Serratula and Klasea genera was included. The special attention was paid to the data on chromatographic separation of phytoecdysteroids using column, thin-layer and high-performance liquid chromatography on the various sorbents. The information presented in the review demonstrated the perspectiveness of Serratula and Klasea species as sources of phytoecdysteroids.

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Lee, Sae-Byuk y Heui-Dong Park. "Isolation and Investigation of Potential Non-Saccharomyces Yeasts to Improve the Volatile Terpene Compounds in Korean Muscat Bailey A Wine". Microorganisms 8, n.º 10 (8 de octubre de 2020): 1552. http://dx.doi.org/10.3390/microorganisms8101552.

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The Muscat Bailey A (MBA) grape, one of the most prominent grape cultivars in Korea, contains considerable amounts of monoterpene alcohols that have very low odor thresholds and significantly affect the perception of wine aroma. To develop a potential wine starter for Korean MBA wine, nine types of non-Saccharomyces yeasts were isolated from various Korean food materials, including nuruk, Sémillon grapes, persimmons, and Muscat Bailey A grapes, and their physiological, biochemical, and enzymatic properties were investigated and compared to the conventional wine fermentation strain, Saccharomyces cerevisiae W-3. Through API ZYM analysis, Wickerhamomyces anomalus JK04, Hanseniaspora vineae S7, Hanseniaspora uvarum S8, Candida railenensis S18, and Metschnikowia pulcherrima S36 were revealed to have β-glucosidase activity. Their activities were quantified by culturing in growth medium composed of different carbon sources: 2% glucose, 1% glucose + 1% cellobiose, and 2% cellobiose. W. anomalus JK04 and M. pulcherrima S36 showed the highest β-glucosidase activities in all growth media; thus, they were selected and utilized for MBA wine fermentation. MBA wines co-fermented with non-Saccharomyces yeasts (W. anomalus JK04 or M. pulcherrima S36) and S. cerevisiae W-3 showed significantly increased levels of linalool, citronellol, and geraniol compared to MBA wine fermented with S. cerevisiae W-3 (control). In a sensory evaluation, the flavor, taste, and overall preference scores of the co-fermented wines were higher than those for the control wine, suggesting that W. anomalus JK04 and M. pulcherrima S36 are favorable wine starters for improving Korean MBA wine quality.

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Weyerstahl, Peter, Christian Christiansen y Helga Marschall. "Terpenes and Terpene Derivatives, XXX. Isolation and Synthesis of Isohumbertiol, the First Naturally Occurring Sesquiterpene Alcohol with a Humbertiane Skeleton". Liebigs Annalen der Chemie 1992, n.º 12 (22 de diciembre de 1992): 1325–28. http://dx.doi.org/10.1002/jlac.1992199201218.

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Chang, Yung-Jin, Seung-Hwan Song, Si-Hyung Park y Soo-Un Kim. "Amorpha-4,11-diene Synthase of Artemisia annua: cDNA Isolation and Bacterial Expression of a Terpene Synthase Involved in Artemisinin Biosynthesis". Archives of Biochemistry and Biophysics 383, n.º 2 (noviembre de 2000): 178–84. http://dx.doi.org/10.1006/abbi.2000.2061.

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KABORE, Ibrahim, Mamounata DIAO, Samson GUENNE, Amana METUOR-DABIRE, Hemayoro SAMA, Alin CIOBICA, Adama HILOU, Martin KIENDREBEOGO y Mamoudou H. DICKO. "Phytochemistry and Alternative use of Sweeteners in Metabolic Diseases". Annals of the Academy of Romanian Scientists Series on Biological Sciences 11, n.º 1 (2022): 109–19. http://dx.doi.org/10.56082/annalsarscibio.2022.1.109.

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The plants native to tropical climates and even southern Africa (5/8) are considerable, which served as a matrix for the isolation and identification of natural sweeteners. These compounds of plant origin have become essential in many fields. From the agronomic industry to the pharmaceutical industry, their use is aimed at combating the supply of glucose and additional calories to consumers who wage a fierce fight against metabolic diseases. In this context, that vascular plants capable of harboring new molecules with similar sweet principles are of interest to researchers. Indeed, these molecules of global interest mainly belong to the terpene, flavonoid (phenolic) and protein structural classes, but are mainly of protein nature (7/9) whose use would not promote an increase in calories or blood sugar in the subjects who would use them. Therefore, this review aims to understand the information regarding, the phytochemistry and the impact of the use of these sweeteners on those affected by food conditions such as diabetes and obesity. All with the aim of finding other sources of sweeteners in our Burkinabe ecosystem, in order to provide local relief to the affected populations.

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Maksimovic, Svetolik, Vanja Tadic, Jasna Ivanovic, Tanja Radmanovic, Stoja Milovanovic, Milica Stankovic y Irena Zizovic. "Utilization of the integrated process of supercritical extraction and impregnation for incorporation of Helichrysum italicum extract into corn starch xerogel". Chemical Industry and Chemical Engineering Quarterly 24, n.º 2 (2018): 191–200. http://dx.doi.org/10.2298/ciceq170223031m.

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Supercritical CO2 extraction of Helichrysum italicum and impregnation of starch xerogels with the extract by using an integrated scCO2 extraction and impregnation process were performed at 350 bar and 40?C in order to produce biomaterials for possible oral intake of the extract. Xerogels produced by air-drying of acetogels and alcogels were used as carriers in the supercritical impregnation process. The effect of ethanol as a co-solvent, contact time, plant material/carrier mass ratio and xerogel preparation on the impregnation loading was studied. The highest impregnation loading (1.26?0.22%) was achieved after 5 h impregnation of the xerogel obtained from alcogel using pure scCO2 and plant material/ /carrier mass ratio of 10. Chemical analysis of the extracts showed that the addition of ethanol as co-solvent had a positive effect on scCO2 selectivity to terpene fraction and total flavonoids, while it lowered the total phenolic content. Despite the difference in chemical composition, both extracts expressed similar antioxidant activity according to the DPPH and FRAP methods. The integrated process was shown to be a feasible method for isolation and incorporation of bioactive components of H. italicum into starch xerogels.

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Zou, Ran, Xin Li, Xiaochen Chen, Yue-Wei Guo y Baofu Xu. "Chemical and biosynthetic potential of Penicillium shentong XL-F41". Beilstein Journal of Organic Chemistry 20 (15 de marzo de 2024): 597–606. http://dx.doi.org/10.3762/bjoc.20.52.

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Penicillium strains are renowned for producing diverse secondary metabolites with unique structures and promising bioactivities. Our chemical investigations, accompanied by fermentation media optimization, of a newly isolated fungus, Penicillium shentong XL-F41, led to the isolation of twelve compounds. Among these are two novel indole terpene alkaloids, shentonins A and B (1 and 2), and a new fatty acid 3. Shentonin A (1) is distinguished by an unusual methyl modification at the oxygen atom of the typical succinimide ring, a feature not seen in the structurally similar brocaeloid D. Additionally, shentonin A (1) exhibits a cis relationship between H-3 and H-4, as opposed to the trans configuration in brocaeloid D, suggesting a divergent enzymatic ring-expansion process in their respective fungi. Both shentonins A (1) and B (2) also feature a reduction of a carbonyl to a hydroxy group within the succinimide ring. All isolated compounds were subjected to antimicrobial evaluations, and compound 12 was found to have moderate inhibitory activity against Candia albicans. Moreover, genome sequencing of Penicillium shentong XL-F41 uncovered abundant silent biosynthetic gene clusters, indicating the need for future efforts to activate these clusters and unlock the full chemical potential of the fungus.

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Smit, Samuel J., Melané A. Vivier y Philip R. Young. "Seeing the Forest through the (Phylogenetic) Trees: Functional Characterisation of Grapevine Terpene Synthase (VviTPS) Paralogues and Orthologues". Plants 10, n.º 8 (26 de julio de 2021): 1520. http://dx.doi.org/10.3390/plants10081520.

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Gene families involved in specialised metabolism play a key role in a myriad of ecophysiological and biochemical functions. The Vitis vinifera sesquiterpene synthases represent the largest subfamily of grapevine terpene synthase (VviTPS) genes and are important volatile metabolites for wine flavour and aroma, as well as ecophysiological interactions. The functional characterisation of VviTPS genes is complicated by a reliance on a single reference genome that greatly underrepresents this large gene family, exacerbated by extensive duplications and paralogy. The recent release of multiple phased diploid grapevine genomes, as well as extensive whole-genome resequencing efforts, provide a wealth of new sequence information that can be utilised to overcome the limitations of the reference genome. A large cluster of sesquiterpene synthases, localised to chromosome 18, was explored by means of comparative sequence analyses using the publicly available grapevine reference genome, three PacBio phased diploid genomes and whole-genome resequencing data from multiple genotypes. Two genes, VviTPS04 and -10, were identified as putative paralogues and/or allelic variants. Subsequent gene isolation from multiple grapevine genotypes and characterisation by means of a heterologous in planta expression and volatile analysis resulted in the identification of genotype-specific structural variations and polymorphisms that impact the gene function. These results present novel insight into how grapevine domestication likely shaped the VviTPS landscape to result in genotype-specific functions.

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WEYERSTAHL, P., C. CHRISTIANSEN y H. MARSCHALL. "ChemInform Abstract: Terpenes and Terpene Derivatives. Part 30. Isolation and Synthesis of Isohumbertiol, the First Naturally Occurring Sesquiterpene Alcohol with a Humbertiane Skeleton." ChemInform 24, n.º 15 (20 de agosto de 2010): no. http://dx.doi.org/10.1002/chin.199315239.

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Alicandri, Enrica, Stefano Covino, Bartolomeo Sebastiani, Anna Rita Paolacci, Maurizio Badiani, Francesco Manti, Carmelo Peter Bonsignore, Agostino Sorgonà y Mario Ciaffi. "Diterpene Resin Acids and Olefins in Calabrian Pine (Pinus nigra subsp. laricio (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes". Plants 10, n.º 11 (5 de noviembre de 2021): 2391. http://dx.doi.org/10.3390/plants10112391.

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A quali-quantitative analysis of diterpenoid composition in tissues obtained from different organs of Pinus nigra subsp. laricio (Poiret) Maire (Calabrian pine) was carried out. Diterpene resin acids were the most abundant diterpenoids across all the examined tissues. The same nine diterpene resin acids were always found, with the abietane type prevailing on the pimarane type, although their quantitative distribution was found to be remarkably tissue-specific. The scrutiny of the available literature revealed species specificity as well. A phylogeny-based approach allowed us to isolate four cDNAs coding for diterpene synthases in Calabrian pine, each of which belonging to one of the four groups into which the d3 clade of the plants’ terpene synthases family can be divided. The deduced amino acid sequences allowed predicting that both monofunctional and bifunctional diterpene synthases are involved in the biosynthesis of diterpene resin acids in Calabrian pine. Transcript profiling revealed differential expression across the different tissues and was found to be consistent with the corresponding diterpenoid profiles. The isolation of the complete genomic sequences and the determination of their exon/intron structures allowed us to place the diterpene synthase genes from Calabrian pine on the background of current ideas on the functional evolution of diterpene synthases in Gymnosperms.

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Klimek, Katarzyna, Katarzyna Tyśkiewicz, Malgorzata Miazga-Karska, Agnieszka Dębczak, Edward Rój y Grazyna Ginalska. "Bioactive Compounds Obtained from Polish “Marynka” Hop Variety Using Efficient Two-Step Supercritical Fluid Extraction and Comparison of Their Antibacterial, Cytotoxic, and Anti-Proliferative Activities In Vitro". Molecules 26, n.º 8 (19 de abril de 2021): 2366. http://dx.doi.org/10.3390/molecules26082366.

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Given the health-beneficial properties of compounds from hop, there is still a growing trend towards developing successful extraction methods with the highest yield and also receiving the products with high added value. The aim of this study was to develop efficient extraction method for isolation of bioactive compounds from the Polish “Marynka” hop variety. The modified two-step supercritical fluid extraction allowed to obtain two hop samples, namely crude extract (E1), composed of α-acids, β-acids, and terpene derivatives, as well as pure xanthohumol with higher yield than that of other available methods. The post-extraction residues (R1) were re-extracted in order to obtain extract E2 enriched in xanthohumol. Then, both samples were subjected to investigation of their antibacterial (anti-acne, anti-caries), cytotoxic, and anti-proliferative activities in vitro. It was demonstrated that extract (E1) possessed more beneficial biological properties than xanthohumol. It exhibited not only better antibacterial activity against Gram-positive bacteria strains (MIC, MBC) but also possessed a higher synergistic effect with commercial antibiotics when compared to xanthohumol. Moreover, cell culture experiments revealed that crude extract neither inhibited viability nor divisions of normal skin fibroblasts as strongly as xanthohumol. In turn, calculated selectivity indexes showed that the crude extract had from slightly to significantly better selective anti-proliferative activity towards cancer cells in comparison with xanthohumol.

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Umaña, Eduardo, Godofredo Solano, Gabriel Zamora y Giselle Tamayo-Castillo. "Costa Rican Propolis Chemical Compositions: Nemorosone Found to Be Present in an Exclusive Geographical Zone". Molecules 28, n.º 20 (14 de octubre de 2023): 7081. http://dx.doi.org/10.3390/molecules28207081.

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Background: The chemistry of Costa Rican propolis from Apis mellifera remains underexplored despite its potential applications. This study identified its chemical composition, linking chemotypes to antioxidant potential. Methods: Proton nuclear magnetic resonance (1H NMR) spectra were obtained for 119 propolis extracts and analyzed using multivariate analyses. In parallel, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was used to assess antioxidant activity. A generalized linear regression model (GLM) correlated this with its chemical profiles and geographical origin. Chromatographic methods were used to isolate active and inactive compounds, which were identified using nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS). Results: Principal component analysis (PCA) revealed three chemical profile groups for the 119 propolis extracts, explaining 73% of the total variance with two components. Radical scavenging activity was found to correlate with chemical composition. Isolation yielded n-coniferyl benzoate in type I (EC50 = 190 µg/mL, ORAC = 0.60 µmol TE/µmol) and nemorosone in type II (EC50 = 300 µg/mL, ORAC = 0.7 µmol TE/µmol). Type III was represented in terpene-like components, which exhibited lower antioxidant activity. Conclusions: This study categorizes Costa Rican propolis into three chemical types and identifies two key components linked to antioxidant activity. Notably, nemorosone, a valuable natural product, was found to be highly concentrated in a particular region of Costa Rica.

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Tran, Thi Diem Huong, Ngoc Tuyet Nguyen y Thi Hong Thuong Nguyen. "Isolation and in silico analysis of a transcript variant for a putative terpene synthase (HbTPS6L-X1) from the bark tissues of rubber Hevea brasiliensis RRIV 209". Ministry of Science and Technology, Vietnam 65, n.º 12 (25 de diciembre de 2023): 70–76. http://dx.doi.org/10.31276/vjst.65(12).70-76.

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Hevea brasiliensis is the only tree species grown to produce natural rubber. The method of harvesting latex by slicing a groove into the bark of the tree mimics a mechanical wounding. In addition, rubber trees could be frequently exposed to pest and pathogen attacks through the scraped bark region. Terpenoids are a group of specialised compounds that play an important role in plant-environment interactions, especially in plant response to stress factors. The diversity of terpenoids is mainly determined by enzymes of the terpene synthase (TPS) family. Screening the RNA-seq data of H. brasiliensis revealed the presence of many putative TPS genes expressed in the bark tissues of rubber trees; however, their function has not been studied. This paper presents the identification of a TPS gene transcript variant (designated as HbTPS6L-X1) from the bark tissues of H. brasiliensis RRIV 209. Through the analysis of the phylogenetic tree, the presence of conserved motifs, and the signal peptide sequence region of the protein, as well as structural homology to known sesquiterpene synthases from other plants, HbTPS6L-X1 was predicted to belong to the TPS-a subfamily, be localised in the cytoplasm and catalyse the conversion of farnesyl diphosphate (FPP) to sesquiterpenes. These in silico functional characteristics are the basis for designing experiments to confirm the in planta biological function of HbTPS6L-X1 protein.

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Hsu, Chih-Yao, Pung-Ling Huang, Chih-Ming Chen, Chi-Tang Mao y Shu-Miaw Chaw. "Tangy Scent in Toona sinensis (Meliaceae) Leaflets: Isolation, Functional Characterization, and Regulation of TsTPS1 and TsTPS2, Two Key Terpene Synthase Genes in the Biosynthesis of the Scent Compound". Current Pharmaceutical Biotechnology 13, n.º 15 (10 de diciembre de 2012): 2721–32. http://dx.doi.org/10.2174/138920112804724864.

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Sundaraj, Yasotha, Hasdianty Abdullah, Nima Ghahremani Nezhad, Kenneth Francis Rodrigues, Suriana Sabri y Syarul Nataqain Baharum. "Cloning, Expression and Functional Characterization of a Novel α-Humulene Synthase, Responsible for the Formation of Sesquiterpene in Agarwood Originating from Aquilaria malaccensis". Current Issues in Molecular Biology 45, n.º 11 (10 de noviembre de 2023): 8989–9002. http://dx.doi.org/10.3390/cimb45110564.

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This study describes the cloning, expression and functional characterization of α-humulene synthase, responsible for the formation of the key aromatic compound α-humulene in agarwood originating from Aquilaria malaccensis. The partial sesquiterpene synthase gene from the transcriptome data of A. malaccensis was utilized for full-length gene isolation via a 3′ RACE PCR. The complete gene, denoted as AmDG2, has an open reading frame (ORF) of 1671 bp and encodes for a polypeptide of 556 amino acids. In silico analysis of the protein highlighted several conserved motifs typically found in terpene synthases such as Asp-rich substrate binding (DDxxD), metal-binding residues (NSE/DTE), and cytoplasmic ER retention (RxR) motifs at their respective sites. The AmDG2 was successfully expressed in the E. coli:pET-28a(+) expression vector whereby an expected band of about 64 kDa in size was detected in the SDS-PAGE gel. In vitro enzyme assay using substrate farnesyl pyrophosphate (FPP) revealed that AmDG2 gave rise to two sesquiterpenes: α-humulene (major) and β-caryophyllene (minor), affirming its identity as α-humulene synthase. On the other hand, protein modeling performed using AlphaFold2 suggested that AmDG2 consists entirely of α-helices with short connecting loops and turns. Meanwhile, molecular docking via AutoDock Vina (Version 1.5.7) predicted that Asp307 and Asp311 act as catalytic residues in the α-humulene synthase. To our knowledge, this is the first comprehensive report on the cloning, expression and functional characterization of α-humulene synthase from agarwood originating from A. malaccensis species. These findings reveal a deeper understanding of the structure and functional properties of the α-humulene synthase and could be utilized for metabolic engineering work in the future.

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Iyekowa, Osaro y Mary Olire Edema. "Chemosuppressive activities in in vivo studies of Plasmodium falciparum-infected mice using isolated oil of Stigmaphyllon ovatum (Amazon vine) Cav." Ovidius University Annals of Chemistry 28, n.º 1 (23 de febrero de 2017): 1–6. http://dx.doi.org/10.1515/auoc-2017-0001.

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AbstractProblem: In sub-Saharan Africa, malaria remains one of the leading health problems. This situation has been aggravated by the increasing spread of drug-resistant Plasmodium falciparum strains. The study was conducted to determine the chemosuppressive activities in in vivo studies of Plasmodium falciparum-infected mice with isolated oil of Stigmaphyllon ovatum leaves used in the traditional treatment of malaria in Nigeria. Methodology: The plant leaves were collected, dried, pulverized and extracted in Soxhlet extractor with hexane solvent. The crude extract was concentrated using a rotary evaporator and phytochemical screening performed using standard methods. Isolation of oil from hexane extract was done using vacuum liquid chromatography while characterization was done by gas chromatography-mass spectrometry (GC-MS). Chemosuppressive activities were conducted along with quinine to determine the antimalarial potency in Plasmodium falciparum- infected BALB/c albino mice. Findings: Glycosides, saponins, phenolics, and alkaloids among others were present. Components detected from the isolated yellow oil of S. ovatum were 9-octadecenoic acid (oleic acid) (Rt:20.0, 19.37%), an unsaturated fatty acid, squalene (Rt:25.6, 4.58%), a terpene; 7-tetradecenal (Rt:22.6, 2.40%), an aldehyde and alicyclic compounds like bicycle (3,10) hexan-3-one (Rt:16.7, 0.22%). Quinine-treated mice exhibited the lowest parasite counts of 0.27±0.01 (83.82% mean chemosuppression) at day 4 of therapy while the lowest parasite counts for the isolated oil was 0.95±0.05 (42.92% mean chemosuppression) at day 4 of therapy. Conclusion: The chemosuppressive activities revealed that the isolated oil exhibit significant suppression (P<0.05) of Plasmodium falciparum when compared with the standard, quinine which was evident by the photomicrograph results. This work corroborates the local use of the plants for the treatment of malaria in Southern Nigeria.

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Abbott, Eric, Dawn Hall, Bjoern Hamberger y Joerg Bohlmann. "Laser microdissection of conifer stem tissues: Isolation and analysis of high quality RNA, terpene synthase enzyme activity and terpenoid metabolites from resin ducts and cambial zone tissue of white spruce (Picea glauca)". BMC Plant Biology 10, n.º 1 (2010): 106. http://dx.doi.org/10.1186/1471-2229-10-106.

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