Tesis sobre el tema "Stress response pathways"
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Crowley, Cara Leilani. "Bile salt induced stress response pathways". Diss., The University of Arizona, 2000. http://hdl.handle.net/10150/289231.
Texto completoBouman, Lena. "A role of parkin in stress response pathways". Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-120918.
Texto completoMutavchiev, Delyan Rumenov. "Regulation of fission yeast cell polarity by stress-response pathways". Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/29006.
Texto completoChalmers, Fiona. "Improving protein yield from mammalian cells by manipulation of stress response pathways". Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7666/.
Texto completoRichards, Siân Louise. "The involvement of Arabidopsis thaliana Annexin 1 in abiotic stress response pathways". Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648626.
Texto completoEdwards, Clare B. "The effects of supplemented metabolites on lifespan and stress response pathways in Caenorhabditis elegans". Scholar Commons, 2015. http://scholarcommons.usf.edu/etd/5681.
Texto completoRyan, Ellis Louise. "Investigating the role of TAB182 in the DNA damage response and replication stress pathways". Thesis, University of Birmingham, 2016. http://etheses.bham.ac.uk//id/eprint/6741/.
Texto completoWeatherbee, Jessica L. "Exploiting DNA Repair and ER Stress Response Pathways to Induce Apoptosis in Glioblastoma Multiforme: A Dissertation". eScholarship@UMMS, 2016. https://escholarship.umassmed.edu/gsbs_diss/865.
Texto completoMelgar, Katelyn M. "A polypharmacologic strategy for overcoming adaptive therapy resistance in AML by targeting immune stress response pathways". University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1571061798761171.
Texto completoWeatherbee, Jessica L. "Exploiting DNA Repair and ER Stress Response Pathways to Induce Apoptosis in Glioblastoma Multiforme: A Dissertation". eScholarship@UMMS, 2008. http://escholarship.umassmed.edu/gsbs_diss/865.
Texto completoDiedrichs, Danilo Roberto. "A mathematical model of the unfolded protein response to stress in the endoplasmic reticulum of mammalian cells". Diss., University of Iowa, 2012. https://ir.uiowa.edu/etd/3284.
Texto completoViana, Tiago Monteiro Lomba. "In search of role of yeast regulatory pathways during the last stages of wine fermentation". Doctoral thesis, ISA/UL, 2014. http://hdl.handle.net/10400.5/7321.
Texto completoWhile searching for roles of Saccharomyces cerevisiae regulatory pathways during the late stages of wine fermentation, a peculiar H+ homeostasis was found in an industrial wine strain, while fermenting natural grape must (NGM). Intracellular pH (pHi) of the population remained in the range 6.0 – 6.4, decreasing by the end of glucose fermentation (pHi 5.2 at 15oC), although cells remained viable. The plasma membranes of late stationary cells became almost impermeable to H+. To ensure replicability of subsequent experiments, a synthetic grape must was designed (ISA-SGM), in which we obtained similar fermentative profiles as in NGM for the wine strain and for laboratory strains. Fluorescence Ratio Imaging Microscopy (FRIM) was used to estimate pHi of individual cells of strain BY4741. Even at the end of fermentation, we found subpopulations with “healthy” cytosolic pH (i.e. pHi 6-7). Sixty-two single-deletion mutants of BY4741 were screened to identify genes required for optimal wine fermentation performance, leading to the identification of 10 determinants whose absence improved fermentation performance, while 37 were deleterious. Principal Component Analysis revealed that deletion of some genes involved in transcription, cell cycle and stress response led to improved performance, while reduced performance was associated mainly with genes involved in membrane functions.
Gomez, Vargas Javier Alejandro. "Regulation of the signal transduction pathways of the unfolded protein response during chronic and physiological ER stresses". Diss., University of Iowa, 2016. https://ir.uiowa.edu/etd/5760.
Texto completoPlatara, Maria. "Signaling pathways regulating the transcriptional response of the sodium ATPase ENA1 to saline and alkaline stress in the yeast Saccharomyces cerevisiae". Doctoral thesis, Universitat Autònoma de Barcelona, 2008. http://hdl.handle.net/10803/3586.
Texto completoEn un estudio previo se identificaron dos regiones del promotor de ENA1 responsables de su respuesta al álcali, la ARR1 (Alkaline Responsive Region 1) que es calcineurina-dependiente y ARR2 que es calcineurina-independiente. En este trabajo restringimos la región responsable de la respuesta alcalina de ARR2 a un pequeño fragmento de 42 nucleótidos que denominamos MCIR (por Minimum Calcineurin Independent Response). MCIR contiene un elemento MIG, capaz de unir a los represores Mig1 y Mig2. Observamos que la respuesta a pH alcalino de la MCIR se anula en células que carecen de Snf1, la quinasa que regula la actividad represora de Mig1 en función de la disponibilidad de glucosa. En cambio, su respuesta se ve moderadamente reducida en cepas rim101, mientras que el doble mutante mig1 mig2 presenta altos niveles de expresión a pH alcalino. Además, la deleción de NRG1 resulta en una expresión elevada y la inducción de MCIR es marginal en el cuádruple mutante nrg1,2 mig1,2. También demostramos que Nrg1 se une al extremo 5' de la ARR2 in vitro e in vivo. Por lo tanto, la respuesta de ENA1 que es calcineurina independiente esta regulada por Rim101 (a través de Nrg1) y por Snf1 (a través de Nrg1 y Mig2). De esta manera, la inducción del promotor de ENA1 por pH alcalino en un mutante rim101snf1 en presencia del inhibidor químico de la calcineurina FK506 se anula totalmente. Por lo tanto, la respuesta transcripcional de ENA1 a estrés alcalino, integra tres vías de señalización, cuya importancia relativa es Snf1 > calcineurina > Rim101.
La CK2 es una quinasa que está conservada en eucariotas y participa en diversos procesos celulares. En S. cerevisiae cepas que carecen de las subunidades reguladoras Ckb1 y/o Ckb2 de la CK2 son muy sensibles a cationes de litio y de sodio.
En este estudio confirmamos observaciones anteriores que describían que la respuesta de ENA1 a estrés salino y alcalino está disminuida en células que carecen de Ckb1 y/o Ckb2. Además demostramos que los mutantes ckb son sensibles a pH alcalino. Las tres vías de señalización (Rim101, calcineurina, Snf1) responsables de la regulación de ENA1 en condiciones de estrés alcalino se examinaron para posibles interacciones con la CK2. Nuestros resultados sugieren que CK2 y calcineurina regulan la expresión de ENA1 de manera independiente. Además, mostramos que la deleción de RIM101 resulta en inducción de la expresión de ENA1, disminuida en condiciones de estrés salino, y que la deleción simultanea de CKB agrava solo ligeramente el defecto de las cepas rim101 en la expresión salina y alcalina de ENA1. Deleción del factor de transcripción Nrg1 en un fondo genético ckb resulta en niveles de expresión de ENA1 relativamente altos en condiciones de estrés salino y alcalino. Estos resultados, junto con datos anteriores que muestran que Nrg1 se fosforila por CK2 en estas condiciones de estrés, son compatibles con una supuesta interacción entre CK2 y la vía Rim101. Cabe destacar que la deleción de CKB repara el defecto que presentan las células snf1 en la expresión de ENA1 bajo estrés salino y alcalino y que los mutantes ckb1,2 snf1 presentan un crecimiento en litio mayor que la cepa salvaje, sugiriendo la existencia de una interacción compleja entre CK2 y Snf1.
The adaptive response of the yeast Saccharomyces cerevisiae to environmental alkalinization results in remodeling of gene expression. A key target is the gene ENA1, encoding a sodium ATPase, whose induction by alkaline pH was shown to integrate at least two different signals, mediated by the calcineurin and the Rim101 pathways.
Early work in our laboratory identified two regions in the ENA1 promoter required for full response to alkalinization, the ARR1 (from Alkaline Responsive Region 1), whose response is calcineurin-dependent and ARR2, whose response is calcineurin-independent. In this work we have restricted the alkaline response of ARR2 to a smaller fragment of 42 nucleotides that we denominated MCIR (from Minimum Calcineurin Independent Response). MCIR contains a MIG element, able to bind Mig1 and Mig2 repressors. We observe that high pH-induced response driven from MCIR is largely abolished in cells lacking Snf1, the protein kinase that regulates repressor activity of Mig1 with respect to glucose availability, and results moderately reduced in a rim101 strain, whereas the double mig1 mig2 mutant presents high levels of expression upon alkaline stress. In addition, deletion of NRG1 results in increased expression and induction from the MCIR region is marginal in a quadruple nrg1,2 mig1,2 mutant. We also demonstrate that Nrg1 binds to the 5´-end of the ARR2 region in vitro and in vivo. Therefore, the calcineurin-independent response of the ENA1 gene is under the regulation of Rim101 (through Nrg1) and Snf1 (through Nrg1 and Mig2). Accordingly, induction by alkaline stress of the entire ENA1 promoter in a snf1 rim101 mutant in the presence of the calcineurin inhibitor FK506 is completely abolished. Thus, the transcriptional response to alkaline stress of the ENA1 gene integrates three different signaling pathways, whose relative potency is Snf1 > calcineurin > Rim101.
CK2 is a well conserved kinase among eukaryotes that participates in many different cellular processes. In Saccharomyces cerevisiae, strains lacking the regulatory subunits Ckb1 and/or Ckb2 of this kinase are hypersensitive to sodium and lithium cations. However, the mechanism by which CK2 affects yeast salt tolerance is not known.
In this study we confirm previous observations that the alkaline and saline response of ENA1 is decreased in strains lacking Ckb1 and/or Ckb2. Furthermore, we show that ckb mutants are sensible at alkaline pH. The three pathways (Rim101, calcineurin and Snf1) responsible for ENA1 regulation under alkaline stress conditions were examined for any possible interaction with CK2. Our results suggest that CK2 and calcineurin regulate ENA1 expression under alkaline and lithium stress in an independent fashion. Moreover, we show that deletion of RIM101 results in decreased ENA1 induction under lithium stress conditions and that simultaneous mutation of CKB only slightly aggravates the defect that presents the rim101 strain in ENA1 alkaline and saline expression. Mutation of the Nrg1 transcription factor in a ckb background leads to relatively high levels of ENA1 expression under alkaline and lithium stress conditions. These results, together with previous data showing that Nrg1 is phosphorylated by CK2 under these stress conditions, support a possible interaction between the CK2 and the Rim101 pathways. Remarkably, deletion of CKB partially counteracts the defect that snf1 mutants present in ENA1 saline and alkaline expression, and ckb1,2 snf1 mutants are as tolerant as wild type cells to lithium ions, revealing a complex interaction between CK2 and Snf1.
Xing, Qikun. "Deciphering the oxylipin signaling pathways during defense responses in brown macroalgae". Thesis, Sorbonne université, 2021. http://www.theses.fr/2021SORUS536.
Texto completoOxylipins are oxygenated compounds derived from polyunsaturated fatty acids (PUFAs), found in many organisms. In land plants and animals, they are known to have regulating roles in growth and in response to various stresses. Brown algae have developed unique oxylipin pathways, using both C18- and C20- type PUFAs. However, the role and regulation of oxylipin pathways during defense responses are largely unclear in brown algae. In my PhD thesis, I conducted large transcriptomic analyses in order to decipher the molecular responses of two kelps species, Saccharina latissima and Laminaria digitata during biotic interactions, in relationships with the induction of oxylipin pathways. The transcriptomic analysis was performed on a kelp-endophyte co-cultivation bioassay and oligoguluronate elicitation. Among differentially expressed genes, several genes putatively involved in oxylipin pathways were identified with diverse expression patterns. During the elicitation, one putative cytochrome P450 (CYP)-encoding gene was induced at 1 h and 12 h, suggesting that oxylipin pathways in S. latissima might be induced at least two times. The result of metabolite profiling showed an early production of putative C18 oxylipins and the putative alternative role of C20 oxylipin pathways during the elicitation. Finally, the biological function of several oxylipins and aldehydes were tested on targeted gene or metabolite inductions in S. latissima and L. digitata. Altogether, these results led to the partial reconstruction of oxylipin pathways and provide a better understanding of their regulation during defense responses in kelps
Packer, John. "Identification of signalling pathways involved in the oxidative stress response triggered by Low Temperature Plasma in prostate epithelial cells and the assessment of tumour-associated allelic expression in prostate cancer". Thesis, University of York, 2018. http://etheses.whiterose.ac.uk/22237/.
Texto completoKoren, John. "The Role of Hsp70 in Cancer: A Study of the Hsp70 / Akt Relationship". Scholar Commons, 2012. http://scholarcommons.usf.edu/etd/4105.
Texto completoNaim, Adnan. "The Role of G3BPs in the Stress Response Pathway". Thesis, Griffith University, 2016. http://hdl.handle.net/10072/367499.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Natural Sciences
Science, Environment, Engineering and Technology
Full Text
Nalam, Vamsi J. "9-Lipoxygenase Oxylipin Pathway in Plant Response to Biotic Stress". Thesis, University of North Texas, 2012. https://digital.library.unt.edu/ark:/67531/metadc115127/.
Texto completoGomes, Susana Isabel Lopes. "Effect assessment of nanoparticles toxicity in the terrestrial compartment". Doctoral thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/12578.
Texto completoOver 11 million tons of nanomaterials (NMs) have been produced in 2012 and predictions point the increase in production. Despite predictions and extended usage via consumer products and industry, the understanding of the potential impact of these materials on the environment is virtually absent. The main aim of this thesis is to understand how a selected group of nanomaterials (metal based particles) may impact soil invertebrates, with special focus on the mechanisms of response. Since a case-by-case Environmental Risk Assessment (ERA) of all the emerging contaminants (particularly NMs) is impossible, among others due to time and cost reasons, to gain understanding on the mechanism of action and response is very important to reach a common paradigm. Understanding the modes of action provides predictive characters in cross particle extrapolation. Besides, it also provides insight for the production of new and sustainable materials. Overall, the effects of the selected NMs (Copper and Silver, Titanium and Zirconium oxides) and the respective salt forms, were investigated at the gene expression (using high-throughput tools, microarray and qPCR technology), biochemical (using enzymatic assays for analysis of oxidative stress markers) and organism (survival and reproduction as in OECD test guidelines) levels, this using standard soil species (Enchytraeus albidus, Enchytraeus crypticus, Eisenia fetida). Gene expression analysis provided valuable information on the mechanisms affected by each of the NMs. The gene expression profile highlighted a (nano)material signature and the effect of the duration of exposure. The functional analyses integrated with the biochemical and organism data, revealed a good understanding power. The biochemical parameters (oxidative stress related) were distinct across the materials and also influenced by duration of exposure and concentration. The standardized organismal responses differed the least between the various materials. The overall outcome is that, in this context of NMs effect assessment, gene expression and enzymatic assays introduced a very important knowledge gap, which could not had been achieved by the standard organismal effects alone. A reoccurring issue with some metal based NMs is the possible dissolution and subsequent release of ions that then causes toxicity e.g. Cu-NPs or Ag-NPs release Cu2+ or Ag+. The oxidation state of the particles was investigated, although this was not the focus of the thesis. The study of fate, e.g. dissolution of NPs, is also only in its beginning and the appropriate techniques are currently being developed. The results showed a specific nanoparticle effect. The UV exposure with titanium dioxide nanoparticles increased its effect.
Em 2012 foram produzidas mais de 11 milhões de toneladas de nanomateriais (NMs) e as perspetivas apontam para um aumento na produção. Apesar das previsões e o uso extensivo em produtos de consumo e indústria, o conhecimento é praticamente inexistente no que diz respeito ao potencial impacto destes materiais no ambiente. O principal objetivo desta tese é compreender o impacto de um grupo de NMs selecionados (NMs de base metálica) em invertebrados de solo, com especial incidência nos mecanismos de resposta. Uma vez que a avaliação de risco ambiental feita caso-a-caso para todos os contaminantes emergentes (particularmente NMs) é impossível, devido, entre outros fatores, ao tempo e custos necessário, a compreensão dos mecanismos de ação é muito importante para alcançar paradigmas comuns. A compreensão dos modos de ação fornece os caracteres com valor preditivo para a extrapolação entre partículas. Além disso, também fornece informação para a produção de novos materiais sustentáveis. Em suma, os efeitos dos NMs selecionados (Cobre e Prata, Óxido de Titânio e Zircónio) e do respetivo sal, foram investigados ao nível dos genes (utilizando a ferramentas de alto varrimento, tecnologia de “microarrays” e PCR em tempo real), bioquímico (utilizando ensaios enzimáticos para a análise de marcadores de stress oxidativo) e do organismo (sobrevivência e reprodução, tal como nos protocolos OCDE), utilizando espécies modelo ecotoxicológicas (Enchytraeus albidus, Enchytraeus crypticus, Eisenia fetida). A análise da expressão de genes forneceu informação importante sobre os mecanismos afetados por cada um dos NMs testados. Os perfis de expressão genéticos evidenciaram uma assinatura do (nano)material e o efeito do tempo de exposição. A análise funcional integrada com os dados bioquímicos e de organismo revelou um bom poder de entendimento. As respostas dos parâmetros bioquímicos (relacionados com stress oxidativo) foram distintas entre os materiais testados e também influenciados pelo tempo de exposição e concentrações testadas. As respostas padronizadas ao nível do organismo foram as que mostraram menor diferenciação entre os vários materiais testados. De um modo geral, e neste contexto de avaliação de efeitos de NMs, a expressão de genes e ensaios enzimáticos, apresentaram um papel muito importante no preenchimento de lacunas que não podería ter sido alcançado através dos efeitos no organismo isoladamente. Um assunto recorrente relativo a alguns NMs de base metálica tem a ver com a possível dissolução e subsequente libertação de iões que a posteriori causam toxicidade, p.e. Cu-NPs ou Ag-NPs libertam Cu2+ ou Ag+. O estado de oxidação das partículas foi investigado, apesar deste não ser o foco da tese. O estudo do destino, p.e. dissolução de NPs, está ainda apenas no seu início e as técnicas apropriadas estão presentemente a ser desenvolvidas. Os resultados mostraram um efeito específico das nanopartículas. A exposição UV com o dióxido de titânio aumentou o seu efeito.
Johnson, Shane Benjamin. "An analysis of prefrontal cortex pathways and their assembly of stress coping responses". Diss., University of Iowa, 2019. https://ir.uiowa.edu/etd/6965.
Texto completoMorancho, Armisen Beatriz. "Regulation of NFAT5 by signaling pathways involved in osmotic stress responses and cell growth". Doctoral thesis, Universitat Pompeu Fabra, 2008. http://hdl.handle.net/10803/77906.
Texto completoAl-Bader, Nadia. "The role of the trehalose biosynthesis pathway in «Aspergillus fumigatus» development and stress response". Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=40760.
Texto completoLe tréhalose est un disaccharide de glucose qui fait partie du métabolisme d’organismes comme les bactéries, les plantes, les insectes et les champignons, mais il est absent chez les mammifères. Chez les champignons, le tréhalose sert de source d’énergie pour la reproduction et la croissance, alors qu’il peut conférer une protection aux cellules en conditions de stress environnemental. En plus, l’accumulation de tréhalose a été liée à la virulence chez les levures pathogènes. Normalement, la biosynthèse de tréhalose est effectuée par un complexe de protéines comprenant une tréhalose-6-phosphate synthéase, une tréhalose-6-phosphate phosphatase et des protéines régulatrices. Chez la moisissure pathogène Aspergillus fumigatus, on a identifié quatre gènes potentiels de tréhalose-6-phosphate synthéases : tpsA, tpsB, tpsC et tpsD. Nous avons observé une accumulation de tréhalose pendant le cycle de vie de A. fumigatus et elle a été accompagnée par une expression considérable de tpsA et tpsB. Le tréhalose s’est accumulé suite à un choc thermique et cette accumulation correspondait à une expression accrue de tpsA. On a donc fait une disruption de tpsA, tpsB et de ces deux gènes chez A. fumigatus. Seulement le double mutant ΔtpsAB n’a pas accumulé de tréhalose. Ce mutant démontrait un délai de germination à température de croissance normale. Ce délai était plus marqué à température élevée à laquelle les spores de ΔtpsAB ont démontré un défaut grave de viabilité, de croissance et de sporulation subséquente. En plus, on a des preuves que ΔtpsAB a un défaut dans la paroi cellulaire. Des études sont en cours chez un modèle de souris pour déterminer si ΔtpsAB a un défaut de virulence, ce qui pourrait faire des tréhalose synthéases une nouvelle cible pour la thérapie antifongique.
Wong, Ee Tsin. "The impact of cell architecture on activation and output of the p53 stress response pathway". Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2006. http://wwwlib.umi.com/cr/ucsd/fullcit?p3225303.
Texto completoTitle from first page of PDF file (viewed September 21, 2006). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references.
Prochnik, Simon Edward. "The role of the fission yeast Wis1 pathway in stress response and cell cycle control". Thesis, University of Edinburgh, 1998. http://hdl.handle.net/1842/11284.
Texto completoFarabaugh, Kenneth Thomas kt. "Insights into a Novel Signaling Pathway that Determines Cell Fate in Response to Hyperosmotic Stress". Case Western Reserve University School of Graduate Studies / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=case1571692276973131.
Texto completoBotha, Cornelis Petrus. "Simulation of the human energy system / Cornelis Petrus Botha". Thesis, North-West University, 2002. http://hdl.handle.net/10394/9623.
Texto completoPhD (Mechanical Engineering) North-West University, Potchefstroom Campus, 2003
Bain, Peter A. y n/a. "Gene Expression Profiling of Cylindrospermopsin Toxicity". Griffith University. School of Biomolecular and Physical Sciences, 2007. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20080404.145834.
Texto completoBain, Peter A. "Gene Expression Profiling of Cylindrospermopsin Toxicity". Thesis, Griffith University, 2007. http://hdl.handle.net/10072/367068.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Physical Sciences
Faculty of Science, Environment, Engineering and Technology
Full Text
Schwaiger, Marion [Verfasser] y Markus [Akademischer Betreuer] Heinrichs. "Long-term consequences of childhood adversity – exploring stress responsive molecular pathways and a psychobiological intervention model". Freiburg : Universität, 2016. http://d-nb.info/1124005110/34.
Texto completoWang, Qunwei. "ATM kinase and DNA-PKcs pathways regulate the expression of IL-23 in dendritic cells : interaction with ER-stress pathways and impact on Th17 responses". Thesis, University of Nottingham, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.718993.
Texto completoWei, Yu-Chen. "Pct1 regulates phosphatidylcholine synthesis in response to changes in surface curvature elastic stress sensed on the inner nuclear membrane". Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/287931.
Texto completoEvans, Joanne Susan. "A role for the stress-activated MAPK pathway of Schizosaccharomyces pombe in the cellular response to ionising radiation". Thesis, University of Oxford, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.410679.
Texto completoBourougaa, Karima. "Diversifying the p53 pathway in response to Endoplasmic Reticulum stress via alternative translation initiation of the p53 mRNA". Paris 7, 2009. http://www.theses.fr/2009PA077128.
Texto completoThe p53 protein is the product of a tumour suppressor gene mutated in 50% of human tumours and it has been suggested that all tumours carry defects in the p53 pathways. The p53 protein is a tetrameric transcription factor which is activated in response to a large panel of stresses. P53 downstream pathways control Gl and G2 cell cycle arrest, DNA repair or apoptosis. Even though a large number of p53 response genes have been identified it is still not clear how cells can differentiate the cell biological outcome of p53 activation. More recently, several p53 isoforms hâve been identified and the physiological roles of these are now starting to unravel. The p53/47 isoform lacks the first 39 amino acids including the Mdm2 binding site and trans-actiyation domain I. In consequence, p53/47 homo and hetero-oligomer complexes exhibit altered activity and biochemical properties. The results presented here show that activation of the PERK kinase in response to endoplasmic reticulum (ER) stress induces cap-independent translation of p53/47 via an Internai Ribosome Entry Site (IRES) located within the first 120 nt of the p53 encoding sequence. ER stress promotes selective homo-oligomerisation of p53/47 which induces 14-3-3a and a G2 arrest. At the same time, p53/47 prevents p53-induced expression of p21 and Gl arrest. This thesis shows how IRES-dependent mRNA translation in response to a specific cellular stress pathway alters the expression of p53/47 allowing the cell to differentiate the cell biological outcome of p53 activation. This study can then help to unravel how thé cells can differentiate the cell biological response to different types of cell stress
Machingura, Marylou. "Activity of the beta-cyanoalanine synthase pathway is associated with the response to abiotic stress by Arabidopsis thaliana". OpenSIUC, 2012. https://opensiuc.lib.siu.edu/dissertations/628.
Texto completoAlvarenga, Santos Buiate Ester. "ESTABLISHMENT OF BIOTROPHY BY THE MAIZE ANTHRACNOSE PATHOGEN COLLETOTRICHUM GRAMINICOLA: USE OF BIOINFORMATICS AND TRANSCRIPTOMICS TO ADDRESS THE POTENTIAL ROLES OF SECRETION, STRESS RESPONSE, AND SECRETED PROTEINS". UKnowledge, 2015. http://uknowledge.uky.edu/plantpath_etds/17.
Texto completoFlanagan, Marc David. "The roles and regulation of ubiquitin/ubiquitin-like protein conjugation pathways in responses to oxidative stress in Schizosaccharomyces pombe". Thesis, University of Newcastle Upon Tyne, 2013. http://hdl.handle.net/10443/1825.
Texto completoLeitao, Beatriz Belchior. "The role of the SUMO pathway in the cellular responses to oxidative stress signals in human endometrium". Thesis, Imperial College London, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.544293.
Texto completoWang, Hong. "Regulation of the plant one-carbon metabolic pathway and global gene responses in maize under salt stress". Diss., The University of Arizona, 2001. http://hdl.handle.net/10150/289752.
Texto completoAmarsaikhan, Nansalmaa. "The Role Of The Cross Pathway Control Protein In The Stress Response And Adaptation Of Aspergillus Species To Antifungals". Phd thesis, METU, 2010. http://etd.lib.metu.edu.tr/upload/12612308/index.pdf.
Texto completoMorris, Bethany H. "The Effect of Stress on Hedonic Capacity in Generalized Anxiety Disorder: A Prospective Experimental Study of One Potential Pathway to Depression". Scholar Commons, 2009. https://scholarcommons.usf.edu/etd/1716.
Texto completoOun, Rabiaa R. Salah. "The Rad9.M50 variant of the DNA damage checkpoint protein Rad9 regulates the MAP kinase pathway in the response to heat stress". Thesis, Bangor University, 2016. https://research.bangor.ac.uk/portal/en/theses/the-rad9m50-variant-of-the-dna-damage-checkpoint-protein-rad9-regulates-the-map-kinase-pathway-in-the-response-to-heat-stress(507c63ee-2f27-4f3d-a39b-e7dba7201f95).html.
Texto completoWeerakoon, Tasmeen Shiny. "Investigation of a putative mitochondrial Twin Arginine Translocation pathway in Arabidopsis thaliana". Miami University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1501256746410956.
Texto completoKeiten-Schmitz, Jan [Verfasser], Stefan [Akademischer Betreuer] Müller, Volker [Gutachter] Dötsch y Stefan [Gutachter] Müller. "Characterizing the SUMO-targeted ubiquitin ligase pathway in the proteotoxic stress response / Jan Keiten-Schmitz ; Gutachter: Volker Dötsch, Stefan Müller ; Betreuer: Stefan Müller". Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2021. http://d-nb.info/1229989196/34.
Texto completoCornes, Maragliano Eric 1987. "A functional study of the conserved LSM proteins in C. elegans reveals their involvement in the stress response of metazoans". Doctoral thesis, Universitat Pompeu Fabra, 2015. http://hdl.handle.net/10803/315473.
Texto completoLas proteínas de la familia Lsm están conservadas desde bacterias a humanos y participan en el metabolismo de ARN. Aunque el estudio de sus funciones ha sido generalmente abordado mediante aproximaciones bioquímicas en modelos unicelulares, sus funciones en organismos multicelulares son desconocidas. Su estudio en organismos modelo es de especial relevancia biomédica ya que la alteración específica de ciertas proteínas Lsm ha sido relacionada con el desarrollo del cáncer. Mediante el estudio funcional de las once proteínas Lsm presentes en C. elegans, mostramos cómo los genes lsm-1 y lsm-3, pese a no ser esenciales para la viabilidad del organismo, son necesarios para el mantenimiento de su salud. Además, LSM-1 y LSM-3 funcionan durante la respuesta a estrés promoviendo la agregación citoplasmática de proteínas LSM y contribuyendo a la correcta señalización a través de la vía de la Insulina/IGF-like, importante en la regulación del metabolismo y la respuesta a estrés en metazoos. Este estudio destaca el importante papel fisiológico de las proteínas LSM en el desarrollo y la adaptación al estrés de un organismo multicelular.
Ivanova, Petkova Mima. "Functional, genomic and molecular characterisation of Mtl1, an element of the CWI pathway of Saccharomyces cerevisiae with a role in the oxidative stress response". Doctoral thesis, Universitat de Lleida, 2011. http://hdl.handle.net/10803/51089.
Texto completoSaccharomyces cerevisiae es un microorganismo eucariota que se utiliza como modelo de estudio de las vías de transducción de señal implicadas en la respuesta a estrés oxidativo. Hasta el momento no se ha descrito la existencia de una ruta específica de señales de oxidación. Mtl1 es un miembro de la ruta CWI (Cell Wall Integrity: vía de integridad celular) que funciona como un sensor transmembrana que detecta el estrés oxidativo. En el presente estudio se demuestra que Mtl1 es esencial en el proceso de señalización del estrés oxidativo y quiescencia hacia la ruta CWI y hacia el factor general de respuesta a estrés Msn2/Msn4. En este último caso la señalización ocurre a través de Rom2 y Rho1 (y probablemente también a través de Pkc1) hacia la inhibición de las funciones Tor1 y Ras2. La función Mtl1 se requiere para: i) la represión de la trascripción de genes ribosomales, ii) la inducción del factor transcripcional Msn2/Msn4 y iii) activar la ruta CWI en respuesta a estrés oxidativo y ayuno de glucosa. En la segunda parte de la presente tesis se muestra que el dominio citoplasmático de Mtl1 interacciona físicamente con Rom2, la GEF (GTP Exchange Factor: factor intercambiador de GTP). Nuestros datos sugieren que la actividad Slt2 es importante para la supervivencia en condiciones de quiescencia. Sin embargo, Msn2/Msn4 contribuyen de manera más significativa a la supervivencia celular frente a condiciones oxidativas. Además, la ausencia de TOR1 o RAS2 es suficiente como para inducir la activación de Slt2 de manera independiente de Mtl1, en las condiciones de estrés mencionadas anteriormente. Todo ello sugiere que entre CWI, TOR y RAS-cAMP se establecen una serie de reacciones cruzadas encaminadas a asegurar que las células sean capaces de adaptar el crecimiento y su maquinaria metabólica de manera adecuada. Mtl1 se N-glicosila y se O-manosila, principalmente por la manosil transferasa Pmt2. Mtl1 se localiza preferentemente y de manera homogénea en la periferia celular, yema, septo y en la punta del shmoo. La manosilación de Mtl1 es importante para la localización de Mtl1 de manera regular en la periferia y en la punta del shmoo. La O-manosilación catalizada por Pmt2 en general, y en particular la O-manosilación de Mtl1, poseen una gran relevancia en: a) la respuesta a estrés oxidativo; b) frente al bloqueo en la ruta TOR; y c) la extensión cronológica de la vida.
The eukaryotic microorganism Saccharomyces cerevisiae serves as a model system in which to study the signal transduction pathways involved in the oxidative stress response. Up to date, there is no evidence of any MAPK cascade which is specific to oxidative signals. Mtl1 is a member of the CWI pathway, which functions as a cell wall sensor for oxidative stress. In the present study, we propose an essential role for Mtl1 in signalling oxidative stress and quiescence to the CWI pathway and to the general stress response through the inhibition of either Tor1 or Ras2 functions. The Mtl1 function is required i) to induce ribosomal gene repression, ii) to induce the general stress response driven by the transcription factor Msn2/Msn4, and iii) to activate the CWI pathway in response to both oxidative stress and glucose starvation. The signalling from Mtl1 to Tor1 and/or Ras2 inhibition under these conditions occurs through Rom2 and Rho1, and probably through Pkc1, at least that signal which target is the ribosomal gene expression. We demonstrate that the Mtl1 cytoplasmic domain physically interacts with the GEF Rom2. Our data indicate that Slt2 activity is really essential in terms of cell survival in quiescent conditions. However, in response to oxidative stress the contribution of Msn2/Msn4 function is more significant. In addition, we demonstrate that deletion of either TOR1 or RAS2 is sufficient to activate Slt2 upon the above mentioned stress conditions, independently on Mtl1. These data suggested that CWI, TOR and Ras-cAMP provide diverse cross talks in order to assure the cells to appropriately adapt metabolism and growth. We demonstrate that Mtl1 is N-glycosylated and highly O-mannosylated mostly by Pmt2 protein O-mannosyltransferase. Mtl1 localises to the cell periphery, the bud, the septum, and to the tip of the shmoo. Mtl1 O-mannosylation confers its proper localisation. We provide evidence for the importance of protein O-mannosylation in oxidative stress response, through at least Mtl1. This is the first report suggesting a role of protein Omannosylation in cell survival upon TOR blockage. Mtl1 O-mannosylation by Pmt2 is required to elicit cellular responses to TOR inhibition. Both Pmt2 and Mtl1 play positive roles in the chronological life span.
Wu, Hui-Chen. "Molecular bases of the heat shock response in plants : identification of elements involved in HS transduction pathway and in the cross talk between HS and oxidative stress". Thesis, Montpellier 2, 2010. http://www.theses.fr/2010MON20125.
Texto completoWhile being unable to escape their lands, plants are continuously submitted to the modifications of their environment, and need to adjust proper physiological processes in response to various stimuli. During this work, I devoted my studies on two major stresses affecting plant development, heat shock (HS) and oxidative stresses (OS), focusing on key elements in these pathways (HS chaperons and HS-related thioredoxins) in order to bring news elements of knowledge and interconnexion of these pathways.Using rice and soybean as mono- and dicotyledonous plant systems, I show how HS leads to calcium release from plant cell apoplast to the cytosol in a typical calcium signature, conferring cell wall rigidity and enhancing HS signaling pathway. I also identify Pectin Methylesterase as required in this pathway for cell wall remodeling and plasma membrane integrity. I further investigate how plant sense temperature increases and how they transmit the HS signal to downstream elements. Using systematic analyses of Calmodulin (CaM) and small heat shock protein (sHsp) gene expression, I identify one CaM as a coordinator of HS response, which I characterize as involving specific cytosolic/nuclear isoforms of the sHsp family.I latter perform the molecular analysis of TDX, a Thioredoxin suspected to be involved in heat shock response. I show that TDX interacts with cytosolic/nuclear members of the Hsp70 family in a redox dependent manner, both HS and OS inducing its nuclear relocation, and that TDX is required for both acquired thermotolerance and OS signaling.I finally discuss the data brought by this work and propose models with cross-talks between HS and oxidative stress signaling
Gok, Abdulkerim. "Degradation Pathway Models of Poly(ethylene-terephthalate) Under Accelerated Weathering Exposures". Case Western Reserve University School of Graduate Studies / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=case1449244610.
Texto completoAlmazroue, Hanadi Abdulaali. "Identification, Cloning, and Expression of Tobacco Responsive to Dehydration like Protein (RD22), SBIP-355 and Its Role in SABP2 Mediated SA Pathway in Plant Defense". Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etd/2456.
Texto completoAmoedo, Machi Hugo [Verfasser], Gerhard [Akademischer Betreuer] Braus, Gerhard [Gutachter] Braus, Stefanie [Gutachter] Pöggeler y Ralph [Gutachter] Kehlenbach. "The Aspergillus fumigatus Vap-Vip methyltransferase pathway modulates stress response, secondary metabolism and azole resistance / Hugo Amoedo Machi ; Gutachter: Gerhard Braus, Stefanie Pöggeler, Ralph Kehlenbach ; Betreuer: Gerhard Braus". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2019. http://d-nb.info/1190353644/34.
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