Bibliografías temáticas / Self-cleavage

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Literatura académica sobre el tema "Self-cleavage"

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Publicado: 10 de marzo de 2023

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Artículos de revistas sobre el tema "Self-cleavage"

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Manzi, A. E., H. H. Higa, S. Diaz y A. Varki. "Intramolecular self-cleavage of polysialic acid." Journal of Biological Chemistry 269, n.º 38 (septiembre de 1994): 23617–24. http://dx.doi.org/10.1016/s0021-9258(17)31560-0.

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Agback, Peter, Corine Glemarec, Lee Yin, Anders Sandström, Janez Plavec, Christian Sund, Shun-ichi Yamakage et al. "The self-cleavage of lariat-RNA". Tetrahedron Letters 34, n.º 24 (junio de 1993): 3929–32. http://dx.doi.org/10.1016/s0040-4039(00)79266-5.

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Forster, A. C., A. C. Jeffries, C. C. Sheldon y R. H. Symons. "Structural and Ionic Requirements for Self-cleavage of Virusoid RNAs and trans Self-cleavage of Viroid RNA". Cold Spring Harbor Symposia on Quantitative Biology 52 (1 de enero de 1987): 249–59. http://dx.doi.org/10.1101/sqb.1987.052.01.030.

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Little, J. W. "LexA cleavage and other self-processing reactions." Journal of Bacteriology 175, n.º 16 (1993): 4943–50. http://dx.doi.org/10.1128/jb.175.16.4943-4950.1993.

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McCarthy, Tom J., Melissa A. Plog, Shennen A. Floy, Joshua A. Jansen, Juliane K. Soukup y Garrett A. Soukup. "Ligand Requirements for glmS Ribozyme Self-Cleavage". Chemistry & Biology 12, n.º 11 (noviembre de 2005): 1221–26. http://dx.doi.org/10.1016/j.chembiol.2005.09.006.

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McCarthy, Tom J., Melissa A. Plog, Shennen A. Floy, Joshua A. Jansen, Juliane K. Soukup y Garrett A. Soukup. "Ligand Requirements for glmS Ribozyme Self-Cleavage". Chemistry & Biology 13, n.º 6 (junio de 2006): 683. http://dx.doi.org/10.1016/j.chembiol.2006.06.003.

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van Roosmalen, M. L., J. D. H. Jongbloed, A. Kuipers, G. Venema, S. Bron y J. M. van Dijl. "A Truncated Soluble Bacillus Signal Peptidase Produced in Escherichia coli Is Subject to Self-Cleavage at Its Active Site". Journal of Bacteriology 182, n.º 20 (15 de octubre de 2000): 5765–70. http://dx.doi.org/10.1128/jb.182.20.5765-5770.2000.

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ABSTRACT Soluble forms of Bacillus signal peptidases which lack their unique amino-terminal membrane anchor are prone to degradation, which precludes their high-level production in the cytoplasm ofEscherichia coli. Here, we show that the degradation of soluble forms of the Bacillus signal peptidase SipS is largely due to self-cleavage. First, catalytically inactive soluble forms of this signal peptidase were not prone to degradation; in fact, these mutant proteins were produced at very high levels in E. coli. Second, the purified active soluble form of SipS displayed self-cleavage in vitro. Third, as determined by N-terminal sequencing, at least one of the sites of self-cleavage (between Ser15 and Met16 of the truncated enzyme) strongly resembles a typical signal peptidase cleavage site. Self-cleavage at the latter position results in complete inactivation of the enzyme, as Ser15 forms a catalytic dyad with Lys55. Ironically, self-cleavage between Ser15 and Met16 cannot be prevented by mutagenesis of Gly13 and Ser15, which conform to the −1, −3 rule for signal peptidase recognition, because these residues are critical for signal peptidase activity.
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Pabón-Peña, L. M., Y. Zhang y L. M. Epstein. "Newt satellite 2 transcripts self-cleave by using an extended hammerhead structure". Molecular and Cellular Biology 11, n.º 12 (diciembre de 1991): 6109–15. http://dx.doi.org/10.1128/mcb.11.12.6109-6115.1991.

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Synthetic transcripts of satellite 2 DNA from newts undergo self-catalyzed, site-specific cleavage in vitro. Cleavage occurs within a domain that is similar to the hammerhead domain used by a number of self-cleaving, infectious plant RNAs. The newt hammerhead has a potentially unstable structure due to a stem composed of two base pairs and a 2-nucleotide loop, and unlike other hammerheads that have been studied, it cannot cleave as an isolated unit. Here we show that cleavage by a single newt hammerhead requires additional satellite 2 sequences flanking both ends of the hammerhead domain. We also present a structural model of a truncated satellite 2 transcript which is capable of cleavage. The structure includes an internally looped extension to one of the conserved stems of the hammerhead. By in vitro mutagenesis, the identities of each of the five nucleotides composing one of the internal loops were shown to be critical for cleavage. Additional evidence that the extension stimulates self-cleavage in a manner other than by simply stabilizing the hammerhead is presented.
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Pabón-Peña, L. M., Y. Zhang y L. M. Epstein. "Newt satellite 2 transcripts self-cleave by using an extended hammerhead structure." Molecular and Cellular Biology 11, n.º 12 (diciembre de 1991): 6109–15. http://dx.doi.org/10.1128/mcb.11.12.6109.

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Synthetic transcripts of satellite 2 DNA from newts undergo self-catalyzed, site-specific cleavage in vitro. Cleavage occurs within a domain that is similar to the hammerhead domain used by a number of self-cleaving, infectious plant RNAs. The newt hammerhead has a potentially unstable structure due to a stem composed of two base pairs and a 2-nucleotide loop, and unlike other hammerheads that have been studied, it cannot cleave as an isolated unit. Here we show that cleavage by a single newt hammerhead requires additional satellite 2 sequences flanking both ends of the hammerhead domain. We also present a structural model of a truncated satellite 2 transcript which is capable of cleavage. The structure includes an internally looped extension to one of the conserved stems of the hammerhead. By in vitro mutagenesis, the identities of each of the five nucleotides composing one of the internal loops were shown to be critical for cleavage. Additional evidence that the extension stimulates self-cleavage in a manner other than by simply stabilizing the hammerhead is presented.
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10

Wu, H. N. y M. M. Lai. "RNA conformational requirements of self-cleavage of hepatitis delta virus RNA". Molecular and Cellular Biology 10, n.º 10 (octubre de 1990): 5575–79. http://dx.doi.org/10.1128/mcb.10.10.5575-5579.1990.

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Hepatitis delta virus (HDV) RNA subfragments undergo self-cleavage at varying efficiencies. We have developed a procedure of using repeated cycles of heat denaturation and renaturation of RNA to achieve a high efficiency of cleavage. This effect can also be achieved by gradual denaturation of RNA with heat or formamide. These results suggest that only a subpopulation of the catalytic RNA molecules assumes the active conformation required for self-cleavage. This procedure could be of general use for detecting catalytic RNA activities.
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Tesis sobre el tema "Self-cleavage"

1

Forster, Anthony Carlyle. "Self-cleavage of plant pathogenic RNAs". Title page, contents and summary only, 1987. http://web4.library.adelaide.edu.au/theses/09PH/09phf7331.pdf.

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Sheldon, Candice Claire. "Hammerhead mediated self-cleavage of plant pathogenic RNAs /". Title page, contents and summary only, 1992. http://web4.library.adelaide.edu.au/theses/09PH/09phs544.pdf.

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Dick, Sarah. "Caspase 3 Cleavage of Pax7 Inhibits Self-Renewal of Satellite Cells". Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32233.

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Compensatory growth and regeneration of skeletal muscle is dependent on the resident stem cell population, termed satellite cells. Self-renewal and maintenance of the satellite cell niche is coordinated by the transcription factor Pax7, yet continued expression of this protein inhibits the myoblast differentiation program. As such, the reduction or removal of Pax7 may denote a key prerequisite for satellite cells to abandon self-renewal and acquire differentiation competence. Here, we identify caspase 3 cleavage inactivation of Pax7 as a crucial step for terminating the self-renewal process. Inhibition of caspase 3 results in elevated Pax7 protein and satellite cell self-renewal, while caspase activation leads to Pax7 cleavage and initiation of the myogenic differentiation program. We have also noted that casein kinase 2 (CK2) directed phosphorylation of Pax7 attenuates caspase directed cleavage. Together, these results demonstrate that satellite cell fate is dependent on opposing post-translational modifications of the Pax7 protein.
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4

Gencer, Burcak. "Analysis Of Self-processing Mechanism Of Galactose Oxidase By Site-directed Mutagenesis And Heterologous Expression In Escherichia Coli". Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/3/12607081/index.pdf.

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In this study, self-catalytic maturation of heterologously expressed pro-galactose oxidase was analysed in E.coli by altering some amino acids which were supposed to play a crucial role in pro-peptide removal. Galactose oxidase (GOase
EC 1.1.3.9) from Fusarium graminearum
having a molecular mass of 68kDa, is a monomeric, copper containing enzyme with an unusual thioether bond. The enzyme is produced as a precursor with an additional 8 amino acid pre- and a 17- amino acid pro-sequence at the N terminus. Previous work has shown that the pre-peptide is removed possibly by a protease during secretion, whereas the 17 amino acid pro-peptide is removed autocatalytically by the aerobic addition of Cu2+ to the precursor, preceding the formation of the thioether bond at the active site. The pro-gao gene was on ProGON1 and ProGOMN1 constructs which were previously established on pET101/D/lacZ vector in England by directed evolution. ProGON1 contains silent mutations at the N-terminus different from native galactose oxidase whereas ProGOMN1 has six further mutations within the mature enzyme, providing high expression. The cleavage site mutations R-1P/A1P, R-1X/A1X, S2A, and the H522A mutation just against the cleavage site in the three dimensional configuration, were carried out by site-directed mutagenesis. Those and some extra mutations were confirmed by DNA sequence analysis. Next, mutant galactose oxidases were expressed in E. coli BL21 Star (DE3), and were purified by Strep-Tactin®
Sepharose®
column, operating on the basis of affinity chromatography. Subsequently, SDS-PAGE was performed to analyze self-processing by detecting molecular mass difference of protein bands resulting from pro-sequence removal or existence. When the bands obtained in SDS-PAGE were compared, it was seen that the products of original recombinant plasmids, i.e. ProGON1, ProGOMN1
and the mutational variants showed no difference in band size, all slightly above 70kDa
indicating pro-sequence presence on all constructs. Non-mutants and some of the mutants showed galactose oxidase activity, signifying proper active site construction by thioether bond formation. ProGOMN1 was submitted for N-terminal amino acid sequencing to be able to assert that a size above 70kDa is not solely due to the existence of a 1 kDa Strep-tag II at C-terminus. Sequencing data affirmed the presence of both the pre-peptide and the pro-preptide showing that processing has not occurred at the N-terminus. Accordingly, in this study, it was shown for the first time that the existence of a pre-pro-peptide at the N-terminus of galactose oxidase does not prevent thioether bond formation at the active site. Furthermore, since the pro-peptide is cleaved autocatalytically, the lack of removal of the pre-peptide in E.coli in the presence of Cu 2+ and oxygen is very likely to be the cause of lack of pro-peptide cleavage. In future studies the region corresponding to the pre-peptide will be deleted to prove this hypothesis.
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5

Boiron, Stéphane. "Au sujet du passage à l'acte délinquant chez les adolescents contemporains issus de populations migrantes, quels sont les liens avec l'intégration des parents et l'intégration des jeunes (telles qu'elles sont perçues par les adolescents), le niveau d'estime de soi et l'anxiété". Thesis, Grenoble, 2011. http://www.theses.fr/2011GRENH012/document.

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En prenant le soin de dépeindre un tableau de l'immigration étrangère en France telle qu'on la retrouve de nos jours, nous associons à ce travail descriptif une définition de l'intégration dans la société française contemporaine. Il importe après de présenter le contexte général du passage à l'acte délictueux, puis de décrire des mécanismes psychiques sous-jacents à celui-ci. Ceci nous met alors en mesure de développer des réflexions au sujet de dimensions importantes dans cette recherche clinique, comme par exemple celle du double clivage et celle du stress acculturatif. Au sujet des mécanismes psychiques, nous donnons une large place aux rites d'institution de soi, à la négativité, à l'agressivité, à la violence, au lien groupal et bien sûr au rapport entre la migration et la délinquance. Dans la réflexion sur le double clivage, nous nous arrêtons sur l'appartenance culturelle et sur l'identité, et nous mesurons aussi le lien entre l'histoire (ou l'Histoire) et le clivage dedans-dehors, un lien autour duquel prennent toute leur importance les notions de la filiation, de la transmission, du faux self ou encore de l'entre deux. Ensuite, nous rencontrons des notions telles que la vulnérabilité de l'estime de soi, la distorsion cognitive, la violence individuelle, les préjugés de groupe ou encore les processus d'acculturation. Cela nous amène à considérer les liens entre l'assimilation et l'immigration, en prenant le temps de saisir dès lors l'importance prise par la notion de l'honneur chez les jeunes résidant dans des quartiers dits sensibles et issus de populations migrantes. Cela nous permet de passer à une revue des liens potentiels entre le passage à l'acte délinquant et l'estime de soi, ainsi qu'entre le passage à l'acte délinquant et l'anxiété. La première revue nous amène à traiter notamment de la carence d'élaboration psychique et de son déterminisme dans le passage à l'acte délinquant. La deuxième revue nous donne l'occasion de prendre du temps sur l'analyse du modèle cognitif de traitement de l'information spécifique à l'anxiété, ce qui amène à considérer les notions de l'attention sélective et de l'hyper vigilance, ainsi que celle de la phobie sociale. Une dernière revue nous permet d'évoquer la place influente que prend l'exil dans l'histoire d'un sujet et dans l'histoire de sa famille. Cela n'est pas sans lien sur certaines formes de violence ou encore certaines manifestations délictueuses qui surgissent dans l'espace public comme dans l'espace privé
Taking care to describe a board of the stranger immigration in FRANCE as it is existing nowadays, we connect to this descriptive work a definition of the integration in the contemporary french society. It is important after this to present the general context of the punishable acting out, and then to describe the subjacent psychic mecanisms of these. It authorizes us in this way to spread out reflexions about some importants dimensions in this clinical research, like for example, the "double cleavage". About psychics mecanisms, we accord a big place at rites, agressivity, violence, and of course at the ties between migration and juvenile delinquency. In the reflexion about the "double cleavage", we take a reflexion moment on the cultural appartenance and on the identity, and we also measure the ties between family 's history and the cleavage between "the inside" and "the outside", a bond on which take a big importance the notions of relationship, dependance and transmission, and the fact to navigate between two cultures. Then, we meet notions like the vulnerability of self esteem, the cognitive distorsion, individual violence, prejudiced people, etc. This leads us to consider the ties between immigration and assimilation, taking care to grasp the importance of the notion of honour for youg mens who are living in the slums, and who are coming from migrant people. This work leads us to be interested by the ties between delinquency and self esteem, and by the ties between delinquency and anxiety. The first review leads us to take care about the notion of the default of psychic elaboration and about its acting out so well-defined. The second review gives us the occasion to be interested by the analysis of the cognitive pattern of the treatment of the specific information of anxiety, so, we consider in this way the notions of vigilance and social phobia. A last review authorize us to evoke the influent place taken by the exile in the family story. This should have a tie with some violence forms or some punishable manifestations which are rising in the private space or in the public space
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6

Lin, Yan-Ru y 林妍如. "HDV;promoter-like element;self-cleavage activity". Thesis, 1995. http://ndltd.ncl.edu.tw/handle/k7be57.

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Forster, Anthony Carlyle. "Self-cleavage of plant pathogenic RNAs / by Anthony Carlyle Forster". Thesis, 1987. http://hdl.handle.net/2440/18536.

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賈儒馨. "Inhibition Of Self-Cleavage Acitivity Of Hepatitis Delta Virus Genomic Ribozyme By Amimoglycoside". Thesis, 1995. http://ndltd.ncl.edu.tw/handle/60857002834825094566.

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碩士
國立臺灣大學
微生物研究所
83
Hepatitis delta virus(HDV) genome is a single-stranded circular RNA consisting of about 1700 nucleotides. Both genomic and antigenomic HDV RNAs have self-cleavage activity(ribozyme activity), which is believed to play an important role in RNA processing when the RNAs are synthesized through a rolling-circle mechanism. 　　Recently, aminoglycoside antibiotics have been found to act on functional RNA elements both in 16S rRNA and in the group l intron core. There is no nucleotide sequences similarities can be detected by comparing group l introns and rRNA, implying that a three-dimensional structure is recognized by the antibiotics. In this study, we try to examine whether antibiotics could interact with HDV ribozyme, and then assay their effect on the activity of ribozyme. 　　First, a variety of antibiotics have been selected to examine their effects on the self-cleavage activity on HDV subfragment (HDV nucleotides 654 to 770). We found that a subset of aminoglycoside antibiotics, including neomycin, tobramycin, kanamycin, gentamycin and netromycin, can inhibit the ribozyme activity in vitro. The effect of Mg2+ions on such inhibition was also studied. It has been shown that the concentration of antibiotics required for inhibiting 50% of ribozyme activity, namely ID50, is reduced when the reaction are carried out with lower concentration of Mg2+ions. To further narrow down the HDV ribozyme region involved in antibiotic inhibition, a smaller HDV ribozyme(HDV nt 683 to 770) was tested. The results showed that the ID50 of antibiotics was reduced for the smaller HDV ribozyme. 　　Subsequently, we tried to identify the sites of HDV RNA which interact with antibiotics. By using base-specific chemcial probing methods, we found that the modification pattern at position A704 was changed in the presence of tobramycin. A704 is located at the bottom of stem II which may play a role in promoting correct folding and stabilizing the structure. We therefore suggest that antibiotics could result in conformational change of RNA. Finally, we tested the effect of such inhibitory antibiotics on HDV replication in vivo. In tissue oulture system, no obvious effect of antibiotics on HDV replication was observed.
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Chia, Ju-hsin y 賈儒馨. "Inhibition of self-cleavage activity of hepatitis delta virus genomic ribozyme by aminoglycoside antibiotics". Thesis, 1995. http://ndltd.ncl.edu.tw/handle/73797484532055470978.

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碩士
國立臺灣大學
微生物學研究所
83
Hepatitis delta virus(HDV) genome is a single-stranded circular RNA consisting of about 1700 nucleotides. Both genomic and antigenomic HDV RNAs have self-cleavage activity , which is processing when the RNAs are synthesized through a rolling-circle mechanism. Recently, aminoglycoside antibiotics have been found to act on functional RNA elements both in 16S rRNA and in the group Iintron core. In this study, we try to examine whether the antibiotics could interact with HDV RNA, and then assay their effect on the activity of RNA. First, many antibiotics have been selected to examine their effects of the self-cleavage activity on HDV RNA(HDV nt 654 to 770). We found that a subset of aminoglycosides, including neomycin, tobramycin, kanamycin, gentamycin, and netromycin, can inhibit the ribozyme activity in vitro. The effect of Mg2+ ions on such inhibition was also studied. It has been shown that the concentration of antibiotics required for inhibiting 50% of ribozyme activity,namely ID50, is reduced when the reaction are carried out in lower concentration of Mg2+ ions. To furture narrow down the HDV RNA region involved in antibiotics inhibition, a smaller HDV ribozyme(HDV nt 683 to 770) was tested. The results showed that the ID50 of antibiotics was reduced for the smaller RNA. Subsequently, we tried to identify the sites of HDV RNA which interact with antibiotics. By using chemical probing methods,we found that the modification pattern at A704 was changed in the presence of tobramycin. A704 islocated at the bottom of stem II which play a role in promoting corret folding and stabilizing the structure. We suggest that antibiotics could result in conformational change of ribozyme. Finally, we tested the effect of antibiotics on HDV replication in vivo. In tissue culture system, no obvious effect of antibiotics on HDV replication was observed.
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Brown, Abigail Leigh. "Competing RNA Structures and Their Effects on HDV Antigenomic RNA Self-cleavage and mRNA Processing". Diss., 2010. http://hdl.handle.net/10161/3131.

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HDV antigenomic RNA is processed in two distinct pathways; it can be cleaved at the polyA site and polyadenylated to become mRNA for the delta antigens, or the RNA can be cleaved by the antigenomic ribozyme to become full-length antigenomic RNA that is used for synthesis of genomic HDV RNA. The polyA site is located just 33 nucleotides upstream of the ribozyme cleavage site. If processing occurs primarily at the upstream polyA site, there may not be enough full-length antigenomic RNA to support replication. On the other hand, ribozyme cleavage downstream of the polyA site could inhibit polyadenylation by interfering with polyadenylation complex assembly. Thus, it appears that HDV may need a mechanism to control RNA processing so that both products can be generated in the proper amounts during the infection cycle.

A model has been proposed in which the choice between ribozyme cleavage and polyadenylation is determined by alternative RNA secondary structures formed by the polyA sequence (Wadkins and Been 2002). One of the hypothetical structures, AltP2, is a pairing between part of the upstream polyA sequence and the 3' end of the ribozyme sequence. For this model, the same upstream sequence that forms AltP2 could also form a stem loop, P(-1), within the leader, by pairing with sequences located farther upstream. A processing choice is possible because AltP2 is predicted to inhibit ribozyme cleavage and favor polyadenylation resulting in mRNA production, whereas P(-1) would inhibit polyadenylation and favor ribozyme cleavage resulting in full-length replication product.

The P(-1) vs. AltP2 model was tested using an antigenomic HDV ribozyme construct with the 60-nucleotide sequence upstream of the ribozyme cleavage site. This leader sequence contains the proposed polyA sequence elements. In vitro analysis of this construct revealed that the kinetic profile of ribozyme self-cleavage was altered in two ways. Relative to the ribozyme without upstream sequences, the fraction of precursor RNA that cleaved decreased to about 50%, but the active ribozyme fraction cleaved faster. Native gel electrophoresis revealed that the active and inactive precursor RNAs adopted persistent alternative structures, and structure mapping with Ribonuclease T1 and RNase H provided evidence for structures resembling P(-1) and AltP2.

Sequence changes in the 5' leader designed to alter the relative stability of P(-1) and AltP2 increased or decreased the extent of ribozyme cleavage in a predictable way, but disrupting AltP2 did not completely restore ribozyme activity. The analysis of deletion and base change variants supported a second alternative pairing, AltP4, formed by the pyrimidine-rich sequence immediately 5' of the ribozyme cleavage site and a purine-rich sequence from the 5' side of P4. A similar approach was used to test if the effect of disrupting both AltP2 and AltP4 might be additive, and the results suggested that ribozyme precursors with 5' leader sequences could fold into multiple inactive conformations, which can include, but may not be limited to, AltP2, AltP4, or a combination of both.

Luciferase expression constructs with HDV polyA and ribozyme sequences were used to investigate the effects of RNA structure and ribozyme cleavage on polyadenylation in cells. One hypothesis was that P(-1) could inhibit polyadenylation by making the polyA sequence elements less accessible to polyA factors, but sequence changes designed to alter the stability of the stem loop had no effect on polyadenylation. The model also predicts that the ribozyme sequence downstream of the polyA site could affect polyadenylation, possibly in two different ways. Ribozyme cleavage could interfere with polyadenylation by uncoupling transcription from processing, however, the ribozyme sequence might also influence polyadenylation in a manner independent of the ribozyme cleavage activity. As such, the AltP2 structure could potentially have a positive effect on polyadenylation either by inhibiting ribozyme cleavage or by making the polyA signal sequences more accessible to the polyA factors. To distinguish between the effects of ribozyme cleavage and alternative RNA structures, luciferase expression levels from constructs with an HDV polyA sequence followed by the active wild-type ribozyme or the inactive C76u version of the ribozyme were compared. For the wild-type HDV polyA sequence, the active ribozyme reduced expression, whereas the inactive ribozyme control had no effect on expression. However, for the modified leader sequences, which were efficiently polyadenylated in the absence of ribozyme, there were changes in expression that appeared to be independent of ribozyme cleavage. Based on these findings, two alternative models are proposed. One model predicts that protein factors might affect antigenomic RNA processing, and the other model suggests that additional alternative structures, such as AltP4, might influence the choice between ribozyme cleavage and polyadenylation.


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Libros sobre el tema "Self-cleavage"

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Seven Days to Sex Appeal: How to Be Sexier Without Surgery, Weight Loss, or Cleavage. Andrews McMeel Publishing, 2008.

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Capítulos de libros sobre el tema "Self-cleavage"

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Sheldon, C. C., A. C. Jeffries, C. Davies y R. H. Symons. "RNA Self-Cleavage by the Hammerhead Structure". En Nucleic Acids and Molecular Biology, 227–42. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-84150-7_14.

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Gerlach, W. L. y M. J. Young. "Self-Cleavage Activities from viral Satellite RNAs". En Plant Molecular Biology 2, 67–73. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3304-7_7.

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Bucher, Taina. "Cleavage-Control". En A Networked Self and Platforms, Stories, Connections, 125–43. New York: Routledge, 2018. | Series: A networked self: Routledge, 2018. http://dx.doi.org/10.4324/9781315193434-9.

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Jaccob, Madhavan, Gopalan Rajaraman y Federico Totti. "On the kinetics and thermodynamics of S–X (X = H, CH3, SCH3, COCH3, and CN) cleavage in the formation of self-assembled monolayers of alkylthiols on Au(111)". En Vincenzo Barone, 99–109. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-34462-6_10.

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"Self-Cleavage of RNA". En Encyclopedia of Genetics, Genomics, Proteomics and Informatics, 1781. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_15317.

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Forster, Anthony C., Christopher Davies, Cheryl J. Hutchins y Robert H. Symons. "Characterization of self-cleavage of viroid and virusoid RNAs". En RNA Processing Part B: Specific Methods, 583–607. Elsevier, 1990. http://dx.doi.org/10.1016/0076-6879(90)81153-l.

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Bhattacharyya, Kushal. "Investigation of Strain Effect on Cleavage Fracture for Reactor Pressure Vessel Material". En Simulation Modeling. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.101245.

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Failure mechanism of 20MnMoNi55 steel in the lower self of ductile to brittle transition (DBT) region is considered as brittle fracture but it has been observed from the experimental analysis of stress-strain diagram that clear plastic deformation is shown by the material before failure. Therefore, strain correction is implemented in the cleavage fracture model proposed by different researchers in the lower self of the DBT region with the help of finite element analysis. To avoid a huge number of experiments being performed, Monte Carlo simulation is used to generate a huge number of random data at different temperatures in the lower self of the DBT region for calibration of the cleavage parameters with the help of the master curve methodology. Fracture toughness calculated after strain correction through different models are validated with experimental results for the different probability of failures.
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8

Latham, John A., Arthur J. Zaug y Thomas R. Cech. "Self-splicing and enzymatic cleavage of RNA by a group I intervening sequence". En RNA Processing Part B: Specific Methods, 558–69. Elsevier, 1990. http://dx.doi.org/10.1016/0076-6879(90)81151-j.

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Acikalin Coskun, Kubra, Nazlıcan Yurekli, Elif Cansu Abay, Merve Tutar, Mervenur Al y Yusuf Tutar. "Structure- and Design-Based Difficulties in Recombinant Protein Purification in Bacterial Expression". En Protein Detection [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.103958.

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Protein purification is not a simple task. Yet, overexpression at bacterial systems with recombinant modifications brings further difficulties. Adding a tag, an affinity label, and expressing particular domains of the whole protein, especially hydrophobic sections, make purification a challenging process. Protein folding pattern may perturb N- or C-terminal tag and this terminal preference may lead to poor purification yield. Codon optimization, solvent content and type, ionic conditions, resin types, and self-cleavage of recombinant proteins bring further difficulties to protein expression and purification steps. The chapter overviews problems of protein purification through a small peptide overexpression in bacteria (Recombinant anti-SARS Coronavirus 2 (SARS-Cov-2) Spike protein Receptor Binding Domain (RBD) antibody (Clone Sb#14). The chapter also covers troubleshooting at distinct steps and highlights essential points to solve crucial issues of protein purification.
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Podlesek, Zdravko y Darja Žgur Bertok. "The Escherichia coli SOS Response: Much More than DNA Damage Repair". En Escherichia coli [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.100353.

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The Escherichia coli SOS response is an inducible DNA damage repair pathway controlled by two key regulators, LexA, a repressor and RecA, an inducer. Upon DNA damage RecA is activated and stimulates self cleavage of LexA, leading to, in E. coli, derepresion of approximately 50 SOS genes. The response is triggered by exogenous and endogenous signals that bacteria encounter at a number of sites within the host. Nevertheless, besides regulating DNA damage repair the SOS response plays a much broader role. Thus, SOS error prone polymerases promote elevated mutation rates significant for genetic adaptation and diversity, including antibiotic resistance. Here we review the E. coli SOS response in relation to recalcitrance to antimicrobials, including persister and biofilm formation, horizontal gene tranfer, gene mobility, bacterial pathogenicity, as well SOS induced bacteriocins that drive diversification. Phenotypic heterogeneity in expression of the SOS regulator genes, recA and lexA as well as colicin activity genes is also discussed.
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Actas de conferencias sobre el tema "Self-cleavage"

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YANG, X. M., R. E. GEORGESCU, J. H. LI, W. F. YU y M. L. TASAYCO. "RECOGNITION BETWEEN DISORDERED POLYPEPTIDE CHAINS FROM CLEAVAGE OF AN α/β DOMAIN: SELF-VERSUS NON-SELF-ASSOCIATION". En Proceedings of the Pacific Symposium. WORLD SCIENTIFIC, 1998. http://dx.doi.org/10.1142/9789814447300_0058.

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Putra, Arief Bramanto Wicaksono, Anggri Sartika Wiguna, Achmad Fanany Onnilita Gaffar y Rheo Malani. "Cleavage self : a new concept in reproduction stage of genetic algorithm for rainfall prediction". En 2020 6th International Conference on Science in Information Technology (ICSITech). IEEE, 2020. http://dx.doi.org/10.1109/icsitech49800.2020.9392057.

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Bezensek, Bostjan y John W. Hancock. "Increased Temperature Margins Due to Constraint Loss". En ASME 2003 Pressure Vessels and Piping Conference. ASMEDC, 2003. http://dx.doi.org/10.1115/pvp2003-2007.

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Enhanced levels of toughness associated with constraint loss are related to temperature shifts in the ductile-brittle transition curve. An argument to quantify the temperature shift is developed using self-similarity of near-tip stress fields under small-scale yielding combined with scaling techniques developed by Dodds and co-workers [1,2] for cleavage. The change in the yield stress and hence temperature that give the same stress field at failure in constrained and unconstrained fields has been determined. The procedure is illustrated using the data of Sherry et al [3] for A533B pressure vessel steel. The results are consistent with the empirical expressions proposed by Wallin [4], and enable a discussion of the physical implications for the micro-mechanics of cleavage.
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4

Holguin, Brandon, James Allison, Donghyeon Ryu, Zachary Alvarez, Francisco Hernandez y Jamie Kimberley. "Development of 3D Impact Self-Sensing Composites Using Fracto-Mechanoluminescent EuD4TEA". En ASME 2017 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/smasis2017-3980.

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The objective of this study is to develop three dimensional (3D) impact self-sensing composites capable of localizing impact damage in through-the-thickness direction. The 3D impact self-sensing composites (3D-ISSC) are designed by embedding fracto-mechanoluminescent (FML) crystals in cells of honeycomb-cored fiber reinforced polymer (FRP) structural composites. FML crystals were shown to emit light resulting from cleavage of crystalline structures due to external mechanical stimuli. Unlike other conventional sensor networks, without supplying external electrical source, the 3D-ISSC is envisioned to monitor impact occurrences and detect damage. Instead, the emitted light will be utilized for informing severity of impact occurrences and 3D locations of the impact damage. First, FML europium-doped dibenzoylmethide triethylammonium (EuD4TEA) crystals are synthesized. Second, the synthesized EuD4TEA crystals are embedded in the honey-cored FRP structural composites to fabricate 3D-ISSC. Third, to validate its 3D self-sensing capability, Kolsky bar is employed to apply high strain-rate compressive loading to simulate impact occurrences while taking high-speed video footage for quantifying intensity of FML light emission through image processing technique.
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5

Kovarik, Onrej, Jan Siegl, Jan Cizek, Tomas Chraska y Jan Kondas. "Fracture Toughness of Cold Sprayed Pure Metals". En ITSC2019, editado por F. Azarmi, K. Balani, H. Koivuluoto, Y. Lau, H. Li, K. Shinoda, F. Toma, J. Veilleux y C. Widener. ASM International, 2019. http://dx.doi.org/10.31399/asm.cp.itsc2019p0775.

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Abstract The fracture toughness of pure Al, Cu, Ni, and Ti deposited by cold spraying was investigated to gain a better understanding of the damage process and quantify material performance. Rectangular specimens of self-standing deposits with fatigue pre-cracks were tested in three-point bending. KIC values were obtained from J-R curves and stress-strain curves were plotted. The cold-sprayed deposits exhibited significantly lower fracture toughness than the same wrought materials, and fractographic analysis revealed either ductile or cleavage intergranular fracture as the major failure mode.
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Bae, Jin-Ho, Shi-Hoon Choi, Ki Soo Kim y Ki Bong Kang. "Effect of Thermomechanical Processing Parameters Upon Texture Development and Anisotropy of Mechanical Properties of API-X80 Line Pipe Steels for Spiral-Welded Pipes". En 2004 International Pipeline Conference. ASMEDC, 2004. http://dx.doi.org/10.1115/ipc2004-0278.

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This study has been conducted to analyze the effect of texture and microstructure on the anisotropy of yield strength and Charpy fracture toughness of an X80 line pipe steel. The texture and microstructure were investigated by X-ray diffractometer and electron backscattered diffraction (EBSD). The yield strength and impact energy were measured along 0° (longitudinal), 30° and 90° (transverse) to the rolling direction. It was found that the microstructure of the developed steel consisted of fine acicular and polygonal ferrite with small pearlite and MA constituents. The major components of textures were {332} <113> and {113} <110> orientations. In order to investigate the effect of both morphological and crystallographic texture on yield strength anisotropy, the prediction of the plastic property was carried out by using a visco-plastic self-consistent (VPSC) polycrystal model. The predicted anisotropy of yield strength with VPSC model assuming ellipsoidal grain shape was in a good agreement with experimental observation. EBSD results showed that the density of {100} cleavage planes of Charpy specimen, 30 degree to rolling direction, was the highest compared with that of other specimens. Therefore, the highest susceptibility to the cleavage fracture, i.e. increased ductile-brittle transition temperature, can be seen in the 30 degree direction.
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7

Maclaskey, Sean K., Karthikeyan D. Ramachandriya, C¸ O¨zge Topal, Mark R. Wilkins, Murat Yasar y A. Kaan Kalkan. "Photolytic Nanostructures for Hydrogen Production". En ASME 2011 5th International Conference on Energy Sustainability. ASMEDC, 2011. http://dx.doi.org/10.1115/es2011-54530.

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The present work demonstrates hydrogen generation by nanowire-nanoparticle conjugate structures under visible radiation. The mechanism of hydrogen generation is attributed to photolytic cleavage of water. The nanostructures are synthesized by sol-gel and reduction chemistries and characterized by XRD, SEM, TEM, and optical spectroscopy. Photolysis is analyzed by gas chromatography. We discuss the possible mechanisms involved in the observed photolysis in terms of unique attributes of the multifunctional nanostructures, such as efficient channeling of photocarriers to redox reactions at the interfaces and self-alignment of energy levels leading to efficient charge transfer. We demonstrate conversion and quantum efficiencies of 10.6% and 22.5%, respectively, for the first hour of photolysis, under 470 nm excitation.
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8

Rasponi, Marco, Tania Ullah, Richard Gilbert, Gianfranco B. Fiore y Todd Thorsen. "A Microfluidic Device for Flow-Through Blood Oxygenation by Photocatalytic Action". En ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206652.

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The breakthrough work of Fujishima and Honda in 1972 [1], in which they achieved ultraviolet light-induced water cleavage with the use of titanium dioxide (TiO2) in an electrochemical cell, has drawn considerable attention in recent years to the “acceleration of a photoreaction by the presence of a catalyst” [2] or photocatalysis. Research on photocatalysis has explored the decomposition of organic pollutants and microorganisms, the superhydrophilic self-cleaning properties of surfaces, and the photosplitting of water, among other applications. Semiconductors can act as photocatalysts because of their electronic structure and TiO2, in particular, has been a popular choice. It is non-toxic and mechanically stable, can be fabricated at low-cost, and the anatase phase of TiO2 has a bandgap of approximately 3.2 eV, ideal for excitation by light in the ultraviolet range.
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9

Gollob, Bernhard. "Austria and Artistic Freedom: A Troubled History?" En Mezinárodní konference doktorských studentů oboru právní historie a římského práva. Brno: Masaryk University Press, 2022. http://dx.doi.org/10.5817/cz.muni.p280-0156-2022-9.

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Austria perceives itself as “cultural superpower”. Therefore, it seems fairly surprising that an own fundamental rights provision to protect the arts was positivised only in 1982. The socio-political situation in the perished Austrian Empire and the Weimar Constitution had a decisive impact on attempts to adopt a corresponding provision after World War One. Following the horrors of World War Two, the young Second Republic of Austria used arts and culture as tool to draw the picture of a peace-loving “Kulturnation” sui generis. In the following years a significant cleavage between the Austrian self-perception and the legal-political reality can be observed (also) in regard to Artistic Freedom. Neither Austria’s authoritarian past nor its Nazi past and related crimes had a significant impact on the legislative process.
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10

Lee, K. S., A. H. Sherry y M. R. Goldthorpe. "Residual Stress and Constraint Effects on Fracture in the Transition Temperature Regime". En ASME 2008 Pressure Vessels and Piping Conference. ASMEDC, 2008. http://dx.doi.org/10.1115/pvp2008-61475.

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This paper presents the results of a combined experimental and numerical study aimed at quantifying the influence of self-balancing residual stresses on the fracture toughness constraint benefit of a ferritic pressure vessel steel tested in the cleavage fracture regime. Tests were performed on standard and pre-compressed, high constraint, compact-tension (CT) and low constraint, single-edge-notched tension (SENT) specimens at a temperature close to the Master Curve reference temperature T0. Pre-compression is undertaken prior to pre-cracking to establish a residual stress across the uncracked ligament, which is highly tensile at the pre-crack notch root and balanced by compressive stresses further ahead of the notch. The pre-crack is subsequently introduced into material ahead of the notch, within the tensile residual stress region, specimen by electro-discharge machining and fatigue. The tests demonstrate an influence of tensile residual stresses on the apparent fracture toughness properties for both CT and SENT specimens. The tests on low constraint specimens illustrate the constraint benefit on cleavage toughness for this material, and the influence of residual stresses in reducing this benefit. The paper shows how the observed behaviour can be quantified through using two parameter fracture mechanics. Here, the J-integral is determined by taking full account of the influence of preloading on the crack driving force. Both the elastic-T-stress and the elastic-plastic Q-stress are calculated and demonstrated as constraint indexing parameters. The results demonstrate a reduction in constraint benefit for cracks located within highly bending residual stress fields. Thus, when exploring any possible benefit in fracture toughness due to crack tip constraint, it is critical that the combined influence of the primary and secondary stresses on crack tip constraint be taken fully into account.
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