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Eberts, Carolyn. "The Sanger Brand: The Relationship of Margaret Sanger and the Pre-War Japanese Birth Control Movement". Bowling Green, Ohio : Bowling Green State University, 2010. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=bgsu1268832853.
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Collazo, Verena. "Reverse Sanger-Sequenzierung mittels MALDI-TOF-Massenspektrometrie". [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=964226871.
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Thornley, David John. "Analysis of trace data from fluorescence based Sanger sequencing". Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286265.
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Takara, Alexandre Hideaki. "Padronização da genotipagem da variante G202A da G6PD A- análise comparativa da relação custo-benefício entre TETRA-ARMS e sequenciamento Sanger /". Botucatu, 2018. http://hdl.handle.net/11449/165615.
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Orientador: Paulo Eduardo Martins RibollaResumo: A deficiência da enzima Glicose-6-Fosfato Desidrogenase (G6PD) é uma anormalidade genética de alta prevalência populacional que resulta em uma menor reatividade do sistema de óxido-redução eritrocitário, geralmente sem repercussões clínicas; estima-se que mais de 300 milhões de pessoas são portadoras dessa alteração. A enzima é expressa em todos os tecidos e catalisa a primeira etapa da Via das Pentoses. Nas hemácias, essa via é de fundamental importância na manutenção do equilíbrio de seu estado redox e a deficiência dessa enzima pode favorecer eventos hemolíticos agudos e crônicos; e em recém nascidos, pode contribuir para o agravamento da icterícia neonatal. O diagnóstico da deficiência baseia-se na atividade enzimática, identificada através de testes quantitativos e qualitativos. Os testes qualitativos limitam-se a agrupar indivíduos em “deficientes” e “não deficientes”, já os métodos quantitativos são mais precisos na inferência dessa atividade. Estas técnicas podem necessitar de repetições dos testes para confirmação de resultados incongruentes. Por outro lado, a variante genética responsável pela deficiência pode ser precisamente reconhecida através de testes de diagnóstico molecular. O presente projeto tem como objetivo desenvolver uma metodologia de identificação molecular da variante G202A, frequentemente encontrada na população brasileira, e realizar uma comparação do custo-benefício com a metodologia de sequenciamento de Sanger. Ao todo, foram analisadas 107 amost... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Glucose-6-phosphate dehydrogenase deficiency is a metabolic enzymatic defect affecting 300 million people worldwide. The enzyme is present in all tissues and catalyses the first reaction in the Pentose Phosphate pathway responsible for maintaining the redox equilibrium in red blood cell. Deficient enzyme may lead to acute and chronic haemolytic anaemia and neonatal jaundice. Diagnosis for G6PD deficiency is based on biochemical quantitative or qualitative tests. Qualitative tests only classifies subjects as “deficient” or “non-deficient”, while quantitative tests are more precise, however both biochemical approches need a confirmative assay to confirm ambiguous results. On the other hand molecular identification for the molecular variants are more accurate and precise. We developed a new molecular assay to identify the G202A molecular variant present at high frequency on Brazilian population and comparer it to Sanger sequencing. One hundred and seven peripheral blood sample were collected on filter paper. DNA extraction were performed followed by G6PD exon 4 amplification and sequencing. On-line tool “Primer1” generated allele-specific primers for TETRA-ARMS genotyping. Twenty two subjects were deficient homozygote, eighty four wild homozygote and one heterozygote. All subjects genotype were confirmed by Sanger sequencing. TETRA-ARMS costs per reaction is three times lower than Sanger sequencing. We conclude that TETRA-ARMS is a suitable protocol to detect G202A mutation on h... (Complete abstract click electronic access below)
Mestre
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O'Brien, Joseph Morton. "Transonic Compressor Test Rig rebuild and initial results with the Sanger stage". Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2000. http://handle.dtic.mil/100.2/ADA381019.
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Van, Der Westhuizen Barend Nicolaas. "Aviva Pelham : die sanger as crossover-kunstenaar - 'n ondersoek na die verskynsel". Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/85875.
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Thesis (MMus)-- Stellenbosch University, 2013.ENGLISH ABSTRACT: It is ironic that with so much interest in singing as a career, the possiblities for trained singers are getting less and less, especially in South Africa. With reality programmes such as Idols and Superstars, the popular music world is overflowed with singers with little, if any, voice training. Then there are also singers who try to establish themselves as crossover artists and perform opera arias on stages such as the KKNK Classic Pops and Die Burger’s Afri-Klassiek concerts accompanied by South Africa’s foremost symphony and philharmonic orchestras. But what about the classical singer who cross over into other genres of singing and like some local and international artists have proved to us, musicals could be a genre to explore. This study explore the possiblity of this type of crossover from classical singing to musical singing. By listening to sound recordings and using a series of musical volumes, songs were identified that the classical singer could sing without harming his or her voice technique. One of the success stories of this type of crossover is the Capetonian soprano, Aviva Pelham. In 1972 Pelham established herself as an opera singer in Cape Town and then confused the classical world with her appearance as Eliza Doolittle in 1978. After that she had a successful career on both classical and musical stages. During her career of nearly 40 years as performing artist, Pelham appeared in 28 different opera roles, 11 different operetta roles and 10 musical roles. Aviva Pelham’s achievements were laureated with a Cape Nederburg Opera Prize, four FNB Vita Awards and in 2000 an Arts, Culture and Heritage Award from the Western Cape Minister for Environment and Culture
AFRIKAANSE OPSOMMING: Dit is ironies dat, terwyl daar soveel belangstelling in sang as loopbaan heers, word die geleenthede vir opgeleide sangers meer beperk. In Suid-Afrika veral is die moontlikhede beperk vir klassieke sangers. Met realiteitsprogramme soos Idols en Supersterre is die populêre musiekwêreld vol van sangers met geen of baie min sangopleiding. Daar is dan ook van dié sangers wat probeer om hulself te vestig as crossover-kunstenaars en opera-arias uit te voer op die konsertverhoë, soos bv. die KKNK Classic Pops- en Die Burger se Afri-Klassiek-konserte saam met van Suid-Afrika se voorste simfonie- of filharmoniese orkeste. Maar wat van die die klassieke sanger wat “crossover” in ‘n ander tipe styl van sang en soos deur ‘n paar plaaslike en internasionale kunstenaars al bewys, is musiekblyspele ‘n genre om te ondersoek. Hierdie studie ondersoek dus die moontlikheid van hierdie tipe “crossover” van klassieke sang na musiekblyspele. Daar word deur middel van klankopnames en ‘n reeks volumes van musiekblyspel-liedere, liedere geklassifiseer wat die klassieke sanger kan sing sonder om sogenaamde skade aan sy of haar sangtegniek te doen. Een van die sukses-stories van hierdie tipe “crossover” is die Kaapse sopraan, Aviva Pelham. Pelham het haarself as ‘n operasangeres gevestig in 1972 in Kaapstad en die klassieke wêreld ophol gejaag met haar verskyning as Eliza Doolittle in 1978. Vandaar het sy ‘n suksesvolle loopbaan op beide die klassieke en musiekblyspelverhoë gehad. Pelham het gedurende haar loopbaan, van ongeveer 40 jaar as uitvoerende kunstenaar, 28 verskillende operarolle, 11 verskillende operette-rolle en 10 musiekblyspelrolle vertolk. Aviva Pelham se prestasies is bekroon met ‘n Kaapse Nederburg Operaprys, vier FNB Vita-toekennings en sy is in 2000 vereer deur die Wes-Kaapse Minister vir Omgewings- en Kultuursake met ‘n Kunste, Kultuur en Erfenis-toekenning vir haar bydrae tot die Uitvoerende Kunste.
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Thomas, Julie L. "International intercourse establishing a transnational discourse on birth control in the interwar era (Margaret Sanger) /". [Bloomington, Ind.] : Indiana University, 2004. http://wwwlib.umi.com/dissertations/fullcit/3162269.
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Thesis (Ph.D.)--Indiana University, Dept. of History, 2004.Source: Dissertation Abstracts International, Volume: 66-01, Section: A, page: 0379. Chair: Judith A. Allen. Title from dissertation home page (viewed Oct. 12, 2006).
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Gardiner, A. R. "A genetic investigation of the muscle and neuronal channelopathies : from Sanger to next-generation sequencing". Thesis, University College London (University of London), 2016. http://discovery.ucl.ac.uk/1477237/.
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The neurological channelopathies are a group of hereditary, episodic and frequently debilitating diseases often caused by dysfunction of voltage-gated ion channels. This thesis reports genetic studies of carefully clinically characterised patient cohorts with different episodic neurological and neuromuscular disorders including paroxysmal dyskinesias, episodic ataxia, periodic paralysis and episodic rhabdomyolysis. Genetic and clinical heterogeneity has in the past, using traditional Sanger sequencing methods, made genetic diagnosis difficult and time consuming. This has led to many patients and families being undiagnosed. Here, different sequencing technologies were employed to define the genetic architecture in the paroxysmal disorders. Initially, Sanger sequencing was employed to screen the three known paroxysmal dyskinesia genes in a large cohort of paroxysmal movement disorder patients and smaller mixed episodic phenotype cohort. A genetic diagnosis was achieved in 39% and 13% of the cohorts respectively, and the genetic and phenotypic overlap was highlighted. Subsequently, next-generation sequencing panels were developed, for the first time in our laboratory. Small custom-designed amplicon-based panels were used for the skeletal muscle and neuronal channelopathies. They offered considerable clinical and practical benefit over traditional Sanger sequencing and revealed further phenotypic overlap, however there were still problems to overcome with incomplete coverage. Large custom and non-custom pull-down panels were used to investigate patients with recurrent rhabdomyolysis patients. The contribution of genetic abnormalities was determined, and it was concluded that while the contribution of the RYR1 was substantial, it was minimal for the classic voltage-gated ion channels SCN4A and CACNA1S Lastly, whole-exome sequencing was applied to two large undiagnosed possible channelopathy families. One family was found to indeed harbour a channelopathy mutation, whilst the other did not. Overall, next-generation sequencing proved to be a more thorough and efficient method for channelopathy genetic diagnosis and several novel findings throughout the thesis expanded the current knowledge within the field.
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Karkar, Adnane. "Leucodystrophies : aspects génétiques et moléculaires au Maroc". Thesis, Sorbonne Paris Cité, 2018. http://www.theses.fr/2018USPCC258.
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Les leucodystrophies (LD) sont des troubles héréditaires affectant la substance blanche (SB) du système nerveux central (SNC) avec ou sans atteinte du système nerveux périphérique (SNP). Ces troubles ont en commun des anomalies de la cellule gliale ou de la gaine de la myéline. L’imagerie par résonance magnétique (IRM) représente l’outil majeur pour la détection des anomalies de la SB, ainsi l’IRM accompagnée d’un examen clinique permettent d’orienter le diagnostic étiologique. La confirmation de ce diagnostic est principalement basée sur la biologie moléculaire qui permet la détermination du gène muté. Sachant qu’au Maroc aucune étude sur les leucodystrophies n’avait été réalisée auparavant, nous avons mené une étude prospective afin de déterminer le profil des patients marocains atteints de ces troubles et de mettre en place un diagnostic moléculaire des formes les plus fréquentes. Pour cela nous avons rassemblé des échantillons de familles dont un ou plusieurs membres avaient une suspicion de leucodystrophie. Par la suite nous avons réalisé un séquençage des gènes connu pour leur implication dans les leucodystrophies. Le séquençage s’est déroulé en deux phases. La première, par séquençage Sanger, a concerné les patients présentant un marqueur biochimique positif. La deuxième phase, par séquençage nouvelle génération ou Next-Generation Sequencing (NGS) a concerné les patients sans marqueurs biochimiques apparent. Cette approche nous a permis d’identifier les types de leucodystrophies présentes au sein de la population marocaine, mais aussi de mettre en évidence de nouvelles mutationsLeukodystrophies are hereditary disorders affecting central nervous system white matter (WM) with or without damages in peripheral nervous system. These disorders have in common abnormalities of the glial cell and the sheath of the myelin. Magnetic resonance imaging (MRI) is the major tool to detect WM abnormalities, so MRI in association with a good clinical examination can help to provide an accurate medical diagnosis. Moreover, the confirmation of this or that leukodystrophy remains in the field of molecular biology by the detection of mutated gene translating the phenotype of the patient. Knowing that in Morocco no previous study on leukodystrophies had been carried out, we sought to know the characteristics of Moroccan patients carrying these disorders so as to be able to establish a molecular diagnosis of the most frequent forms. We collected samples from families with one or more members had a suspicion of leukodystrophy. Thus, we carried out a sequencing with an approach that consists in analyzing directly by Sanger method the leukodystrophies having a positive biochemical marker and by next generation sequencing or Next-Generation Sequencing (NGS) leukodystrophies without known biochemical markers. This approach allowed us, to identify leukodystrophies in a sample of the Moroccan population but also to identify new mutations
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Lenhardt, Nina-Veronika Ingeborg [Verfasser] y Bermhard [Akademischer Betreuer] Weber. "Mutationsanalyse in ABCA4-assoziierten Netzhautdystrophien mittels der Didesoxymethode nach Sanger / Nina-Veronika Ingeborg Lenhardt. Betreuer: Bermhard Weber". Regensburg : Universitätsbibliothek Regensburg, 2015. http://d-nb.info/1071713450/34.
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SILVA, D. A. "CARACTERIZAÇÃO de Mutações da Osteogênese imperfeita em Pacientes do Espírito Santo: Estudo Dos genes Ifitm5, Col1a1 e Col1a2". Universidade Federal do Espírito Santo, 2018. http://repositorio.ufes.br/handle/10/7117.
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Previous issue date: 2018-02-27A Osteogênese Imperfeita (OI) é uma doença que ocorre devido à desordem generalizada do tecido conjuntivo causando principalmente fragilidade óssea. Essa desordem, na maioria das vezes, é causada por mutações nos genes produtores das cadeias do colágeno tipo I, COL1A1 ou COL1A2, embora mutações em novos genes envolvidos na via do metabolismo ósseo têm sido constantemente descobertas. Atualmente, existem 17 genes relacionados com esta doença. Um deles é o gene IFITM5, ainda pouco caracterizado na maioria das populações. Por possuir vários genes causadores de OI, o Next Generation Sequencing (NGS), uma técnica de sequenciamento em larga escala, tem sido amplamente utilizada. Contudo, as mutações identificadas por NGS precisam ser validadas para dar confiabilidade aos resultados. Assim, este trabalhou visou identificar mutações no gene IFITM5 e validar mutações identificadas por NGS nos genes COL1A1 e COL1A2 para caracterizar o padrão de mutações nestes genes em pacientes do Espírito Santo. Este estudo contou com uma amostra inicial de 31 pacientes que foram previamente analisados para outros genes. Desta amostra, 8 indivíduos que apresentaram resultados moleculares inconclusivos foram estudados para o gene IFITM5. Foi detectada a presença da mutação c.-14C>T em um paciente. Esta mutação ocorre na região 5-UTR do gene e é recorrente em várias populações do mundo. A validação de mutações foi realizada em 16 indivíduos que apresentaram alterações genéticas nos genes COL1A1 ou COL1A2 detectadas por NGS. Apenas uma das sequências identificadas por NGS não foi validada. Este estudo confirmou que mutações no gene COL1A1 e COL1A2 são encontradas, em aproximadamente 75% dos pacientes, enquanto que no gene IFITM5 são encontradas mutações em, aproximadamente, 3% dos pacientes com OI do Espírito Santo. Esses resultados poderão auxiliar no desenvolvimento de estratégias de diagnóstico molecular mais eficientes para esta doença.
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Alessandrini, Gaia. "Metodi innovativi per il sequenziamento di acidi nucleici". Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amslaurea.unibo.it/7341/.
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Calvani, Nichola Eliza Davies. "Translocation of Fasciola hepatica via international livestock movements: development of ante-mortem molecular diagnostic tools for the identification of Fasciola spp. in livestock". Thesis, The University of Sydney, 2020. https://hdl.handle.net/2123/22464.
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Fasciolosis caused by infection with Fasciola hepatica and Fasciola gigantica is a zoonotic disease of worldwide importance, with an estimated 91 million people considered at risk of infection and livestock losses expected to exceed US $3 billion/year. Despite the significant human and animal health impacts, no test is capable of ante-mortem Fasciola spp. differentiation in areas of parasite sympatry. The aim of this thesis was to design, validate and deploy a suite of highly sensitive molecular diagnostic tools for Fasciola spp. differentiation from faecal samples to enable ante-mortem screening of livestock in Northern Laos. In combination with a traditional sedimentation method and with the use of a high-speed benchtop homogeniser, the detection and quantification of Fasciola spp. infection in 100% of cattle with low faecal egg loads (<25 EPG) was possible. The point of first detection of F. hepatica infection in experimentally-infected sheep was then examined and compared to the sedimentation and a commercially-available coproantigen ELISA. Faecal samples were first considered positive at 6, 7 and 8 weeks post infection (WPI) by coproELISA, real-time PCR and sedimentation, respectively. A simplified method using un-concentrated faecal samples was developed to increase sample throughput. The limit of detection using this method was 10 and 20 EPG for sheep and cattle, respectively. Finally, several single nucleotide polymorphism assays were developed to differentiate Fasciola spp. in faecal samples alongside a Next Generation Sequencing method to determine the contribution of nucleotides from each species. These assays were applied to 153 faecal samples collected from local cattle across 27 villages in Northern Laos to detect F. hepatica translocation in an area of SE Asia with frequent international livestock trade. Of the 91 positive samples, 11 were identified as containing F. hepatica DNA, indicating establishment of the F. hepatica lifecycle in Northern Laos.
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Rocha, Bruno Garcia. "Desenvolvimento de metodologias para identificação molecular do HPV". Universidade Federal de São Carlos, 2016. https://repositorio.ufscar.br/handle/ufscar/8289.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
The Human Papiloma Virus (HPV) is a Sexually Transmitted Disease (STD) very common in the world. It infects the human epithelium may persisting of asymptomatic form or causing some neoplasia. Many studies report the association between HPV and many kinds of cancer such as: lap utero, anus, penis, vagina and vulva. According to INCA data for the year of 2016 are expected 16.340 new cases of lap utero cancer, being the second most frequent case in the female population in Brazil. For the recognition of the virus, there`s a lots of tracking methods, as morphological test (pap test), that observes cytopathic effects caused by the virus on human cells, suggesting the existence of infection, however this type of test presents low results and has shown high taxes of false negative and positive results. To overcome this problems, countless studies has shown the effect of molecular techniques utilization to increase the sensibility and especially, getting recognize and genotyping the HPV virus. On this recent studies, were tested distinct molecular techniques for typing the HPV virus, as Conventional PCR followed by Sanger Sequencing , Real time PCR (SYBRGreen® e Taqman ®) and Sequencing of New Generation. Altogether were collected 318 samples pf cervix grated, and from this material were collected the DNA using an adapted protocol (POWELL; GANNON, 2002). Using the conventional PCR technique followed by Sanger Sequencing we obtained 65 positives samples for the HPV(21%), in 49 samples(75,3%) it was possible to identify the HPV type, in the other 16 samples(24,7%) it was not possible the identification, probably because the infection was formed for two or more types of the virus. With the real time PCR technique using SYBRGreen®, were accomplished an experimente with 30 samples, which was possible to confirm the results in 28 of it, using Sanger Sequencing. In two samples the results are not confirmed, being possible to positive the sample, showing high sensibility of the real time PCR technique. The methodology of New Generation Sequencing (NGS) it showed useful for HPV identification, being one of the first studies published for routine use. And it has great prospects because besides HPV can identify other microorganisms in the sample and quantifies them as well.
O Papilomavírus humano conhecido como HPV é uma doença sexualmente transmissível frequente em todo mundo, ele infecta o epitélio de seres humanos, podendo persistir de forma assintomática ou causar neoplasias. Diversos estudos relatam a associação entre o HPV (Alto Risco) e diversos tipos de câncer como: colo de útero, ânus, orofaringe, pênis, vagina e vulva. Segundo dados do INCA para o ano de 2016, são esperados 16.340 novos casos de câncer de colo de útero, sendo de maior frequência na população feminina no Brasil. Para a identificação do vírus existem inúmeros métodos de rastreio como testes morfológicos (exame do Papanicolau), que observam os efeitos citopáticos que o vírus provoca nas células sugerindo a existência da infecção, mas este tipo de teste apresenta baixa especificidade e vem apresentando altas taxas de falsos-negativos e positivos. Para contornar estes problemas inúmeros estudos têm demostrando a eficácia da utilização de técnicas moleculares, para aumentar a sensibilidade e especificidade, conseguindo identificar e genotipar o vírus do HPV. No presente estudo foram testadas diferentes técnicas moleculares para a identificação do vírus do HPV como: PCR convencional seguida por sequenciamento Sanger, PCR em tempo real (SYBRGreen® e Taqman®) e sequenciamento de nova geração. Ao todo foram coletadas 318 de amostras de raspado do colo cervical. Deste material foi extraído o DNA utilizando um protocolo adaptado (POWELL, GANNON, 2002). Utilizando a técnica da PCR convencional seguida por sequenciamento Sanger obtivemos 65 amostras positivas para o HPV (21%), destas 49 amostras (75,3%) foi possível identificar o tipo do HPV e em 16 casos (24,7%) não foi possível identificar o vírus, sendo possivelmente uma infecção formada por dois ou mais tipos do vírus. Com a técnica de PCR em tempo real utilizando SYBRGreen® foi realizado um experimento com 30 amostras sendo possível confirma o resultado destas com o sequenciamento Sanger em 28 casos. Em duas amostras os resultados não corroboraram, sendo possível positivar a amostra. Mostrando a maior sensibilidade da técnica de PCR em tempo real. A metodologia de sequenciamento de nova geração (NGS) se mostrou útil para identificação do HPV, demonstrada neste trabalho de maneira inédita. O uso do NGS apresenta boas perspectivas pois além do HPV pode identificar outros microrganismos na amostra e quantifica-los.
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Martinsson, Caroline. "Characterisation of EGFR and KRAS mutations in non-small cell lung cancer". Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-126041.
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Background: Lung cancer is the leading cause of cancer-related death and one of the most common cancer types worldwide. Epidermal growth factor receptor (EGFR) has been shown to be an important therapeutic target in non-small cell lung cancer. Kirsten rat sarcoma viral oncogene homologue (KRAS) is a downstream signalling molecule in the EGFR pathway. Lung cancer patients with EGFR mutations respond to tyrosine EGFR inhibitor therapy, in contrast, patients with KRAS mutations do not benefit of such treatment. Methods: This study investigates the frequency of EGFR and KRAS mutations in non-small cell lung cancer patients. Fifty-one lung cancer patients with primary non-small cell lung cancer diagnosed between 1995 and 2005 in the Uppsala-Örebro region were analysed by Sanger sequencing and Pyrosequencing to determine the mutation status of these genes. Results: Five EGFR mutations were found in four patients (8%), two deletions in exon 19, one point mutation in exon 20 and two point mutations in exon 21. KRAS mutations were found in 12 patients (24%), ten codon 12 mutations and two codon 61 mutations. Conclusions: This study confirms previous observations regarding the frequency of EGFR and KRAS mutations in non-small cell lung cancer. Mutations in EGFR and KRAS were mutually exclusive, indicating that both mutations present relevant tumorigenic genomic aberrations.
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Ahluwalia, Sanjam. "CONTROLLING BIRTHS, POLICING SEXUALITIES: A HISTORY OF BIRTH CONTROL IN COLONIAL INDIA, 1877-1946". University of Cincinnati / OhioLINK, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=ucin980270900.
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Lapalombella, Silvana <1982>. "From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amsdottorato.unibo.it/7445/1/Tesi_dottorato_LAPALOMBELLA_ciclo28.pdf.
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In this PhD thesis I will describe different aspects of conservation genetics and genomics of two wild Canidae species, the wolf (Canis lupus) and the golden jackal (Canis aureus), through the study of two of the most variable gene families: the Major Histocompatibility Complex genes (MHC), and Olfactory Receptors genes (OR). In order to perform these studies both Sanger and next generation sequencing (NGS) DNA techniques have been used. The background of the thesis is described in the “General introduction” with phylogeny, classification and evolutionary ecology of the Canidae, with a focus on the species Canis lupus and its main conservation concerns in Italy. Moreover, I will introduce the importance to perform genetic studies as tools for wild-life conservation and management, with a description of the framework of the principal historical and currently used molecular markers that had driven to develop MHC and OR sequencing projects. The thesis is divided into two parts, “PART I – The MHC typing project” and “PART II – The OR genes typing project”. A total of four scientific papers (already published or under revision) will be introduced and illustrated as result of three years of PhD activities at ISPRA’s (Istituto Superiore per la Protezione e la Ricerca Ambientale), Laboratory of conservation genetics, in Ozzano dell’Emilia, and thankfully to a PhD fellowship granted by the Università di Bologna.
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Lapalombella, Silvana <1982>. "From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amsdottorato.unibo.it/7445/.
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In this PhD thesis I will describe different aspects of conservation genetics and genomics of two wild Canidae species, the wolf (Canis lupus) and the golden jackal (Canis aureus), through the study of two of the most variable gene families: the Major Histocompatibility Complex genes (MHC), and Olfactory Receptors genes (OR). In order to perform these studies both Sanger and next generation sequencing (NGS) DNA techniques have been used. The background of the thesis is described in the “General introduction” with phylogeny, classification and evolutionary ecology of the Canidae, with a focus on the species Canis lupus and its main conservation concerns in Italy. Moreover, I will introduce the importance to perform genetic studies as tools for wild-life conservation and management, with a description of the framework of the principal historical and currently used molecular markers that had driven to develop MHC and OR sequencing projects. The thesis is divided into two parts, “PART I – The MHC typing project” and “PART II – The OR genes typing project”. A total of four scientific papers (already published or under revision) will be introduced and illustrated as result of three years of PhD activities at ISPRA’s (Istituto Superiore per la Protezione e la Ricerca Ambientale), Laboratory of conservation genetics, in Ozzano dell’Emilia, and thankfully to a PhD fellowship granted by the Università di Bologna.
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Giacomini, Luana. "Elementos para a organização do trabalho em hemoterapia com vistas à fidelização do doador voluntário de sangue". reponame:Repositório Institucional da FURG, 2007. http://repositorio.furg.br/handle/1/2763.
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Dissertação(mestrado) - Universidade Federal do Rio Grande, Programa de Pós-Graduação em Enfermagem, Escola de Enfermagem, 2007.Submitted by eloisa silva (eloisa1_silva@yahoo.com.br) on 2012-11-12T17:38:02Z No. of bitstreams: 1 luanagiacomini.pdf: 856199 bytes, checksum: efe96955855934b36615c8bc1c5e9a1f (MD5)
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Este estudo de caráter qualitativo e natureza exploratória teve como objetivos detectar a significação do ato de doar sangue; identificar elementos ligados à decisão de tornar-se um doador de sangue; conhecer as dúvidas ligadas à doação de sangue; conhecer a opinião do doador quanto à organização do trabalho realizado pelo serviço; identificar quais estratégias são mais eficientes para o desenvolvimento de um programa de doação voluntária de sangue; identificar a relevância da relação profissional-usuário na conquista de doadores voluntários e habituais. Foi realizado no Banco de Sangue do Hospital Santa Casa da cidade do Rio Grande e os sujeitos foram os doadores que se apresentaram como voluntários, no período de janeiro a março de 2007. Foi solicitado o Consentimento Livre e Esclarecido dos participantes e realizadas entrevistas (semi-estruturadas) para a coleta de dados, as quais foram gravadas e transcritas. A partir do conteúdo das entrevistas, os dados foram classificados em quatro grupos relativos: 1) à significação e o comportamento diante da doação de sangue; 2) à avaliação da organização do serviço e do atendimento ao doador; 3) às estratégias para a promoção da doação de sangue altruísta; e 4) à relação profissional-usuário na fidelização de doadores assíduos. A análise permite perceber que é indispensável desenvolver uma comunicação social eficaz que contemple informação e educação, buscando reduzir os medos, tabus e falsas idéias que podem desmotivar as pessoas a se tornarem doadoras de sangue. Deve-se elaborar ou produzir meios, materiais, mensagens que tenham como função não apenas desmistificar os efeitos da doação, mas, também, despertar a generosidade e solidariedade, e educar quanto à necessidade de doadores saudáveis e regulares. Deve-se explorar os sentimentos dos que doam e não doam para que percebam que a doação se faz de pessoas para pessoas e que, portanto, a responsabilidade pelo abastecimento é de cunho social. Os serviços precisam desenvolver uma filosofia de atendimento aos doadores apoiada na humanização das relações, procurando estar aberto e atento aos questionamentos e responder de forma correta, orientando, acalmando e satisfazendo as necessidades apresentadas pelos doadores. Receber e entender que as queixas podem servir para melhorar o serviço. Desenvolver meios e medir de forma permanente os resultados de indicadores de satisfação que permitam orientar a necessidade de ajustar a prestação da assistência. Também é de extrema valia a implantação de melhorias nos aspectos que estão diretamente relacionados à execução do trabalho para realizar o acolhimento, como ampliação do horário de atendimento, administração da demanda e melhor espaço físico, proporcionando conforto e bem-estar aos usuários.
This study of qualitative character and exploratory nature aimed to detect the significance of the act of donating blood; to identify elements connected with the decision on becoming a blood donator; to know the doubts connected with blood donation; to know the donator’s opinion according to the organization of work done by the service; to identify which strategies are more efficient to the development of a voluntary blood donation program; to identify the relevance of the user-professional relation in the conquest of voluntary and regular donators. It was carried out in the Blood Bank of Santa Casa Hospital in the city of Rio Grande and the subjects were the donators that presented themselves as volunteers, in the period from January to March, 2007. The Statement of Free and Informed Consent of the participants was required and interviews were carried out (semi-structured) for the data collection, which were all recorded and transcribed. From the interview contents, the data were classified in 4 related groups: 1) to the significance and behavior front the blood donation; 2) to the evaluation of the service organization and attention to the donator; 3) to the strategies for the promotion of altruistic blood donation; and 4) to the user-professional relation in the patronization of regular donators. The analysis allows to notice it is indispensable to develop an efficient social communication that includes information and education, seeking for reducing fears, taboos and false ideas which can discourage people to become blood donators. It’s necessary to prepare or produce means, materials, messages that have as a main role not only to desmystify the donation effects, but also, to arouse generosity and solidarity, and to educate about the necessity of regular and healthy donators. It’s necessary to explore the feelings of the ones who donate and the others who do not so that they realize donation is made from people to people and, therefore, the responsibility for the supply is of social nature. The services need to develop a philosophy of attention to the donators based on the humanization of relations, trying to be opened and aware to the questions and answer them in a proper way, orientating, calming and satisfying the needs presented by the donators. Receiving and understanding that complaints may serve to improve the service. Developing means and measuring in a permanent way the indicators of satisfaction results that allow to orientate the necessity of adjusting the giving of assistance. It is also of extreme importance the implementation of improvements on the aspects that are directly related to the work execution to do the reception, such as the increasing of the opening hours, demand administration and better room space, providing comfort and well-being to the users.
Este estudio de carácter cualitativo y naturaleza exploratória tuvo como objetivos detectar el significado del acto de donar sangre; identificar elementos ligados a la decisión de convertirse en un donador de sangre; conocer las dudas relacionadas a la donación de sangre; conocer la opinión del donador al respecto así como sobre la organización del trabajo realizado por el servicio; identificar qué estrategias son más eficientes para el desarrollo de un programa de donación voluntaria de sangre; identificar la importancia de la relación profesional-usuario en la conquista de donadores voluntarios y habituales. Fue realizado em el Banco de Sangre del Hospital Santa Casa de la ciudad de Rio Grande y los sujetos fueron los donadores que se presentaron voluntariamente, en el período de enero a marzo de 2007. Se solicitó el Consentimento Libre y Esclarecido de los participantes y realizadas entrevistas (semiestructuradas) para la colecta de datos, que fueron grabadas e transcriptas. A partir del contenido de las entrevistas, los datos fueron clasificados en 4 grupos relativos: 1) al significado y el comportamiento frente a la donación de sangre; 2) a la evaluación de la organización del servicio y del atendimiento al donador; 3) a las estrategias para la promoción de la donación de sangre altruista; y 4)a la relación profesional-usuario en la fidelidad de donadores asiduos. El análisis permite percibir que es indispensable desenvolver una comunicación social eficaz que contemple información y educación, buscando reducir los miedos, tabus y falsas ideas que pueden desmotivar a las personas a tornarse donadoras de sangre. Se debe elaborar o producir medios, materiales, mensajes que tengan como función no apenas desmistificar los efectos de la donación, pero, também, despertar la generosidad y solidaridad, y educar cuanto a la necesidad de donadores saludables y regulares. Se debe estudiar los sentimientos de los que donan y no donan para que perciban que la donación se hace de personas para personas y que, por lo tanto, la responsabilidad por el abastecimiento es de carácter social. Los servicios necesitan desarrollar una filosofia de atendimiento a los donadores apoyada en el humanitarismo de las relaciones, procurando estar abierto y atento a las interrogaciones y respondiendo de forma correcta, orientando, calmando e satisfaciendo las necesidades presentadas por los donadores. Recibir y entender que las quejas pueden servir para mejorar el servicio. Desarrollar medios y medir de forma permanente los resultados de indicadores de satisfacción que permitan orientar la necesidad de ajustar el atendimiento. También es de extrema valía el implante de mejorias en los aspectos que están directamente relacionados a la ejecución del trabajo para realizar la recepción, con ampliación del horario de atendimiento, administración de la demanda y mejor espacio físico, proporcionando confort y bienestar a los usuarios.
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Delgado, Verdugo Alberto. "Genetic Aspects of Endocrine Tumorigenesis : A Hunt for the Endocrine Neoplasia Gene". Doctoral thesis, Uppsala universitet, Endokrinkirurgi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-224111.
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Endocrine tumors arise from endocrine glands. Most endocrine tumors are benign but malignant variants exist. Several endocrine neoplasms display loss of parts of chromosome 11 or 18, produce hormones and responds poorly to conventional chemotherapeutics. The multiple endocrine neoplasia syndromes are mainly confined to endocrine tumors. This opens the question if there exists a single or several endocrine tumor genes. The aim of the study was to describe genetic derangements in endocrine tumors. Paper I: Investigation of mutational status of SDHAF2 in parathyroid tumors. SDHAF2 is located in the proximity of 11q13, a region that frequently displays loss in parathyroid tumors. We established that mutations in SDHAF2 are infrequent in parathyroid tumors. Paper II: Study of SDHAF2 gene expression in a cohort of benign pheochromocytomas (PCC) (n=40) and malignant PCC (n=10). We discovered a subset of benign PCC (28/40) and all malignant PCC (10/10) with significantly lower SDHAF2 expression. Benign PCC with low SDHAF2 expression and malignant tumors consistently expressing low levels of SDHAF2 were methylated in the promoter region. SDHAF2 expression was restored in vitro after treatment with 5- aza-2-deoxycytidine. Paper III: HumanMethylation27 array (Illumina) covering 27578 CpG sites spanning over 14495 genes were analyzed in a discovery cohort of 10 primary small neuroendocrine tumors (SI-NETs) with matched metastases. 2697 genes showed different methylation pattern between the primary tumor and its metastasis. We identified several hypermethylated genes in key regions. Unsupervised clustering of the tumors identified three distinct clusters, one with a highly malignant behavior. Paper IV: Loss of chromosome 18 is the most frequent genetic aberration in SI-NETs. DNA from SI-NETs were subjected to whole exome capture sequencing and high resolution SNP array. Genomic profiling revealed loss of chromosome 18 in 5 out of 7 SI-NETs. No tumor-specific somatic mutation on chromosome 18 was identified which suggests involvement of other mechanisms than point mutations in SI-NET tumorigenesis. Paper V: The cost for diagnostic genetic screening of common susceptibility genes in PCC is expensive and labor intensive. Three PCC from three patients with no known family history were chosen for exome capture sequencing. We identified three variants in known candidate genes. We suggest that exome-capture sequencing is a quick and cost-effective tool.
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Poulet, Nicolas. "Le sandre (Sander lucioperca (L. )) : biologie, comportement et dynamique des populations en Camargue (Bouches du Rhône, France)". Phd thesis, Université Paul Sabatier - Toulouse III, 2004. http://tel.archives-ouvertes.fr/tel-00008942.
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Le sandre est un percidé largement répandu en Europe. Le fonctionnement de ses populations méridionales est cependant mal connu. L'objectif de ce travail est d'apporter des connaissances nouvelles sur l'écologie du sandre par l'étude de la population d'un réseau de canaux en Camargue (sud de la France). Différentes techniques ont été mises en œuvre pour étudier l'isolement de la population (génétique, morphométrie), l'utilisation de l'habitat (télémétrie), la biologie (traits de vie) et la dynamique dans le système. Le fonctionnement de cette population dépend plus de facteurs biotiques qu'abiotiques (contrairement à ce qui est observé sur les populations septentrionales), ainsi que de facteurs anthropiques (agriculture...)
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Colchen, Tatiana. "Étude multi-traits du cannibalisme intra-cohorte chez les premiers stades de vie du sandre (Sander lucioperca)". Thesis, Université de Lorraine, 2017. http://www.theses.fr/2017LORR0181/document.
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Le cannibalisme intra-cohorte est l’acte de tuer et consommer tout ou partie d’un individu conspécifique du même âge. Longtemps considéré comme un artefact d’élevage, le cannibalisme est maintenant admis comme un phénomène naturel pouvant être soumis à des mécanismes de sélection. Chez les Téléostéens, il est majoritairement référencé au niveau des premiers stades de vie. En condition d’élevage, plusieurs facteurs biotiques et abiotiques ont été testés sans réussir à totalement l’éliminer chez de nombreuses espèces. Dans ce contexte, cette thèse a pour objectifs de mieux comprendre la mise en place et le maintien du cannibalisme, chez le sandre, en se focalisant sur le lien entre cannibalisme, personnalité et mise en place de l’ichtyophagie. Cette thèse a été divisée en trois parties : (i) description du cannibalisme chez les premiers stades de vie du sandre et étude de l’influence de certains facteurs d’élevage, (ii) détermination des traits de personnalité chez les juvéniles, et mise en évidence de l’apparition du comportement piscivore chez les larves, (iii) recherche des liens entre cannibalisme, personnalité et mise en place de l’ichtyophagie. Cette thèse a montré que les facteurs d’élevage testés n’ont pas d’influence sur le cannibalisme. Elle a permis de démontrer l’existence de syndromes comportementaux dès les premiers stades de vie, que l’ichtyophagie ne se mettait pas en place au même âge chez tous les individus et que celle-ci était liée à la personnalité. Nos résultats montrent, également, que le cannibalisme chez les premiers stades de vie du sandre serait lié à la mise en place d’une ichtyophagie précoce et non à une personnalité particulière. On peut en déduire que le cannibalisme ne repose pas, au cours du développement, sur les mêmes caractéristiques individuelles que l’ichtyophagieIntra-cohort cannibalism is the act of killing and consuming the whole, or major part, of a conspecific individual of the same age. Most often considered as an artefact of captive conditions, it is now assumed that cannibalism is a natural phenomenon, which could be submitted to selection. In Teleosts, it is mostly found in early life stages. In rearing conditions, several biotic and abiotic factors have been already tested, yet without successfully stop it in several species. In this context, the aim of this thesis is to better understand the onset and the stability of cannibalism, in pikeperch, by focusing on the link between cannibalism, personality and the onset of ichtyophagy. This study comprised three main parts aiming at: (i) describing the cannibalism in early life stages and studying the impact of rearing factors, (ii) determining personality traits in juveniles and describing the onset of ichtyophagy in larvae, (iii) searching the link between cannibalism, personality and onset of ichtyophagy. This thesis showed that rearing factors have no impact on cannibalism. Furthermore, we found behavioural syndromes in early life stages and that the onset of ichtyophagy did not occur at the same time for all individuals. Our results highlight that cannibalism seems to be linked to the onset of ichtyophagy rather than individual personality. In conclusion, cannibalism is not due, throughout the development, to the same individual characteristics than ichtyophagy
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Stark, Tiara Elizabeth. "Phylogeography and Genetic Diversity of the Commercially-Collected Caribbean Blue-Legged Hermit Crab: Implications for Conservation". The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1543499269936318.
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Müller-Kelwing, Karin. "Curt Sander". Böhlau Verlag, 2020. https://slub.qucosa.de/id/qucosa%3A75069.
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Jönsson, Johanna. "Identification of the tick-borne pathogens Anaplasma phagocytophilum, Neoehrlichia mikurensis and Rickettsia in Swedish ticks : Investigation of transovarial transmission and co-infection". Thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-53366.
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Globally, vector borne diseases cause more than a million deaths each year and more than a billion infections in humans. Ticks are of big medicinal importance since they can transmit pathogens that can cause serious infections. Some recently discovered pathogens that can cause infections in humans are Anaplasma phagocytophilum (A. phagocytophilum) that can cause human granulocytic anaplasmosis (HGA) and Candidatus Neoehrlichia mikurensis (N. mikurensis) that can cause Neoehrlichiosis. It is still widely unknown how prevalent these pathogens are, if ticks can be infected with both of these pathogens and if these pathogens can be transovarially transmitted from adult female to egg and larvae. This study aims to screen for these pathogens in collected ticks from southern Sweden and to detect eventual co-infections and transovarial transmission. A real-time qPCR assay targeting the 16S rRNA gene of N. mikurensis and other Anaplasmataceae was applied on 1356 Ixodes ricinus (I. ricinus) ticks collected from 5 sites in southern Sweden. Positive samples were subjected to Sanger sequencing. A. phagocytophilum occurred in 4.64 % of the ticks, N. mikurensis occurred in 1.33 % of the ticks and also Rickettsia was found to occur in 6.27 % of the ticks. No co-infection was detected. Some samples of tick larvae showed positive results after qPCR, indicating transovarial transmission, but none of the sequences were readable.
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Furgerson, Jessica L. "The Battle for Birth Control: Exploring the Rhetoric of the Birth Control Movement 1914-2014". Ohio University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1420730080.
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Kodipad, Ahad Ahmed. "XPO1 mutations are a novel predictor of shorter time to first treatment in early stage CLL patients". Doctoral thesis, Università del Piemonte Orientale, 2021. http://hdl.handle.net/11579/128430.
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In approximately 70% of newly diagnosed cases, CLL presents at an early clinical stage and is managed with a watch & wait strategy. Until now, few clinical and molecular inform on the risk of treatment requirement. On these grounds, we aimed at identifying new molecular markers that may predict early treatment requirement and may help clinicians to better plan the watch & wait strategy in asymptomatic early stage CLL patients. 295 Binet A CLL patients who referred to our institution were subjected to next-generation-sequencing (NGS) in a panel of recurrently mutated genes in CLL. Two validation multicenter cohorts of 402 treatment naïve Binet A CLL patients (Binet A validation cohort) and 395 untreated Rai 0 CLL patients (Rai 0 validation cohort) were also included and analyzed for XP01 mutations. The primary endpoint was time to first treatment (TTFT). In the training cohort, NGS mutational analysis showed that XP01 was mutated in 7 (2.4%) patients. In univariate analysis, trisomy 12 (p=0.001), unmutated IGHV genes (p<0.0001) and mutations of XP01 (p<0.0001), NOTCHI (p<0.001) and SF3B1 (p=0.022) were associated with a shorter TTFT. By multivariate analysis, XPOI mutations (p=0.002) and unmutated IGHV genes (p<0.0001) maintained an independent association with a shorter TTFT. XPO1 mutational analysis was subsequently investigated in 2 independent multicenter cohorts of early stage CLL
patients. In the Binet A validation cohort (N=402 patients), XPO1 was mutated in 15 (3.7%) patients and was associated with a shorter TTFT (p=0.004). Similarly, also in the Rai 0 validation cohort, (N=395 patients), XP01 was mutated in 8 (2.0%) patients and was associated with a shorter TTFT (p<0.001). By combining the training and the validation cohorts (N=1092 patients), a total of 30 somatically acquired XP01 mutations were identified (2.7% of patients). More precisely, 27
(90.0%) mutations affected XP01 codon E571 and 3 (10.0%) codon D624. From a clinical perspective, patients carrying either XPO1 E571 or D624 mutations showed superimposable outcome in terms of TTFT (p=0.345). Based on these results, XP01 mutational analysis might be incorporated in other prognostic scores and help clinicians to refine the management of the watch and wait strategy for early stage CLL.
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Roche, Jennifer. "Implication des récepteurs de la dopamine dans la régulation de l’axe gonadotrope lors de la période pré-ovulatoire chez le sandre, Sander lucioperca". Thesis, Université de Lorraine, 2018. http://www.theses.fr/2018LORR0234.
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Dans le cadre de la production de nouvelles espèces aquacoles, le sandre, Sander lucioperca, est devenu, depuis plusieurs années, une espèce d’intérêt piscicole en raison de sa valeur économique potentielle. Pour développer et pérenniser sa production aquacole, il est nécessaire de comprendre et maîtriser son cycle de reproduction ainsi que les mécanismes physiologiques mis en jeu afin d’obtenir des œufs et des juvéniles viables tout au long de l’année. Dans cet optique d’optimisation du contrôle du cycle, la dopamine apparaît, chez de nombreux téléostéens dont certains perciformes, comme un inhibiteur de l’axe gonadotrope, via les récepteurs de la famille D2, en bloquant le pulse ovulatoire de LH et l’ovulation. Chez le sandre, le rôle de la dopamine et de ses récepteurs, notamment les récepteurs de la famille D1, est inconnu. L’objet de cette thèse est de déterminer le rôle du système dopaminergique lors des phases finales de l’ovogénèse chez le sandre à travers trois axes principaux : (1) déterminer l’effet du blocage des récepteurs de la dopamine, D1 ou D2, sur la régulation de l’axe gonadotrope et l’induction de l’ovulation en absence et en présence d’une molécule de sGnRHa, (2) définir le répertoire et le profil d’expression des récepteurs dopaminergiques par l’étude du transcriptome cérébral du sandre en période pré-ovulatoire et (3) établir le rôle de la dopamine et de ses différents récepteurs (familles D1 et D2) dans la régulation directe et locale de l’axe gonadotrope aux niveaux cérébral et ovarien. La première partie de ce travail a permis pour la première fois, par l’utilisation d’antagonistes spécifiques des familles de récepteurs D1 et D2, de mettre en évidence un rôle potentiel de la dopamine sur la sécrétion de certains stéroïdes sexuels en période pré-ovulatoire chez le sandre par l’intermédiaire des récepteurs de la famille D1. L’identification de l’ensemble des récepteurs de la dopamine existant chez le sandre nous a permis de confirmer leur expression à tous les niveaux de l’axe gonadotrope (cerveau, hypophyse et ovaires) étayant l’hypothèse d’un rôle de la dopamine dans la reproduction du sandre. Enfin, la dernière partie de ce projet a permis de montrer un rôle régulateur du système dopaminergique, directement au niveau ovarien, sur la production de testostérone par l’intermédiaire des deux familles de récepteurs de la dopamine. L’implication des deux familles de récepteurs a également été mise en évidence dans la production ovarienne de la 17β-estradiol. Au niveau cérébral, seule la famille des récepteurs D2 a été montrée impliquée dans la régulation de l’expression du gène de la GnRH-3. De façon générale, cette étude a permis de mettre en évidence l’implication des récepteurs de la dopamine dans la régulation de l’axe gonadotrope lors des phases finales de l’ovogenèse. Toutefois, des travaux ultérieurs devront être menés pour approfondir les mécanismes physiologiques mis en jeu. D’un point de vue aquacole, les traitements hormonaux à base d’antagonistes des récepteurs de la dopamine ont été inefficaces pour améliorer les performances de reproduction du sandre ce qui n’est pas en faveur de leur utilisation future pour induire l’ovulation chez cette espèce. Ainsi, la mise au point d’autres méthodes d’optimisation sera nécessaire pour continuer à développer la production aquacole du sandrePikeperch, Sander lucioperca, is a potential valuable economic fish, making it a species of interest for aquaculture diversification. In the domestication process, controlling and understanding the reproductive cycle is a crucial step in order to produce viable offspring in a synchronous and predictable way. In many teleosts including some perciforms, dopamine inhibits the ovulatory pulse of LH and the ovulation step through D2 dopamine receptors family. In pikeperch, the roles of dopamine and its receptors, especially those belonging to the D1 receptors family, are unknown. For the purpose of the optimization of pikeperch reproduction, we investigated the role of the dopaminergic system during the final stages of oogenesis in this species: (1) by determining the effects of D1 or D2 receptor antagonists alone or in association with sGnRHa on the regulation of the reproductive axis and on the induction of ovulation, (2) by determining the repertoire and the expression profile of the dopamine receptors using a brain transcriptome analysis during the pre-ovulatory period and (3) by evaluating the role of dopamine and its receptors (D1 and D2 families) in the direct and local regulation of the gonadotropic axis at the brain and ovarian levels. For the first time, we showed that the dopamine/D1 receptors complex regulates the sex-steroids release during the pre-ovulatory period, suggesting that dopamine is involved in pikeperch reproduction. Also, we support its involvement thanks to the identification of the dopamine receptors gene expression at the brain, pituitary and ovarian levels. Finally, we showed that the dopaminergic system directly regulates the ovarian testosterone production, through both D1 and D2 receptor families. The involvement of both dopamine receptor families was also highlighted on ovarian 17β-estradiol production. Only the D2 receptor family was shown to be involved on the brain GnRH-3 gene expression. In conclusion, we point out a dopamine receptors implication on the gonadotropic axis regulation during the final stages of oogenesis in pikeperch. However, further studies should be performed to pinpoint the physiological mechanisms behind this phenomenon. From an aquaculture point of view, hormonal treatments with dopamine receptor antagonists appear to be ineffective to improve pikeperch reproductive performances. Therefore, their use to induce pikeperch ovulation should be put into question and the development of alternative methods is necessary to further promote pikeperch production
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Roche, Jennifer. "Implication des récepteurs de la dopamine dans la régulation de l’axe gonadotrope lors de la période pré-ovulatoire chez le sandre, Sander lucioperca". Electronic Thesis or Diss., Université de Lorraine, 2018. http://www.theses.fr/2018LORR0234.
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Dans le cadre de la production de nouvelles espèces aquacoles, le sandre, Sander lucioperca, est devenu, depuis plusieurs années, une espèce d’intérêt piscicole en raison de sa valeur économique potentielle. Pour développer et pérenniser sa production aquacole, il est nécessaire de comprendre et maîtriser son cycle de reproduction ainsi que les mécanismes physiologiques mis en jeu afin d’obtenir des œufs et des juvéniles viables tout au long de l’année. Dans cet optique d’optimisation du contrôle du cycle, la dopamine apparaît, chez de nombreux téléostéens dont certains perciformes, comme un inhibiteur de l’axe gonadotrope, via les récepteurs de la famille D2, en bloquant le pulse ovulatoire de LH et l’ovulation. Chez le sandre, le rôle de la dopamine et de ses récepteurs, notamment les récepteurs de la famille D1, est inconnu. L’objet de cette thèse est de déterminer le rôle du système dopaminergique lors des phases finales de l’ovogénèse chez le sandre à travers trois axes principaux : (1) déterminer l’effet du blocage des récepteurs de la dopamine, D1 ou D2, sur la régulation de l’axe gonadotrope et l’induction de l’ovulation en absence et en présence d’une molécule de sGnRHa, (2) définir le répertoire et le profil d’expression des récepteurs dopaminergiques par l’étude du transcriptome cérébral du sandre en période pré-ovulatoire et (3) établir le rôle de la dopamine et de ses différents récepteurs (familles D1 et D2) dans la régulation directe et locale de l’axe gonadotrope aux niveaux cérébral et ovarien. La première partie de ce travail a permis pour la première fois, par l’utilisation d’antagonistes spécifiques des familles de récepteurs D1 et D2, de mettre en évidence un rôle potentiel de la dopamine sur la sécrétion de certains stéroïdes sexuels en période pré-ovulatoire chez le sandre par l’intermédiaire des récepteurs de la famille D1. L’identification de l’ensemble des récepteurs de la dopamine existant chez le sandre nous a permis de confirmer leur expression à tous les niveaux de l’axe gonadotrope (cerveau, hypophyse et ovaires) étayant l’hypothèse d’un rôle de la dopamine dans la reproduction du sandre. Enfin, la dernière partie de ce projet a permis de montrer un rôle régulateur du système dopaminergique, directement au niveau ovarien, sur la production de testostérone par l’intermédiaire des deux familles de récepteurs de la dopamine. L’implication des deux familles de récepteurs a également été mise en évidence dans la production ovarienne de la 17β-estradiol. Au niveau cérébral, seule la famille des récepteurs D2 a été montrée impliquée dans la régulation de l’expression du gène de la GnRH-3. De façon générale, cette étude a permis de mettre en évidence l’implication des récepteurs de la dopamine dans la régulation de l’axe gonadotrope lors des phases finales de l’ovogenèse. Toutefois, des travaux ultérieurs devront être menés pour approfondir les mécanismes physiologiques mis en jeu. D’un point de vue aquacole, les traitements hormonaux à base d’antagonistes des récepteurs de la dopamine ont été inefficaces pour améliorer les performances de reproduction du sandre ce qui n’est pas en faveur de leur utilisation future pour induire l’ovulation chez cette espèce. Ainsi, la mise au point d’autres méthodes d’optimisation sera nécessaire pour continuer à développer la production aquacole du sandrePikeperch, Sander lucioperca, is a potential valuable economic fish, making it a species of interest for aquaculture diversification. In the domestication process, controlling and understanding the reproductive cycle is a crucial step in order to produce viable offspring in a synchronous and predictable way. In many teleosts including some perciforms, dopamine inhibits the ovulatory pulse of LH and the ovulation step through D2 dopamine receptors family. In pikeperch, the roles of dopamine and its receptors, especially those belonging to the D1 receptors family, are unknown. For the purpose of the optimization of pikeperch reproduction, we investigated the role of the dopaminergic system during the final stages of oogenesis in this species: (1) by determining the effects of D1 or D2 receptor antagonists alone or in association with sGnRHa on the regulation of the reproductive axis and on the induction of ovulation, (2) by determining the repertoire and the expression profile of the dopamine receptors using a brain transcriptome analysis during the pre-ovulatory period and (3) by evaluating the role of dopamine and its receptors (D1 and D2 families) in the direct and local regulation of the gonadotropic axis at the brain and ovarian levels. For the first time, we showed that the dopamine/D1 receptors complex regulates the sex-steroids release during the pre-ovulatory period, suggesting that dopamine is involved in pikeperch reproduction. Also, we support its involvement thanks to the identification of the dopamine receptors gene expression at the brain, pituitary and ovarian levels. Finally, we showed that the dopaminergic system directly regulates the ovarian testosterone production, through both D1 and D2 receptor families. The involvement of both dopamine receptor families was also highlighted on ovarian 17β-estradiol production. Only the D2 receptor family was shown to be involved on the brain GnRH-3 gene expression. In conclusion, we point out a dopamine receptors implication on the gonadotropic axis regulation during the final stages of oogenesis in pikeperch. However, further studies should be performed to pinpoint the physiological mechanisms behind this phenomenon. From an aquaculture point of view, hormonal treatments with dopamine receptor antagonists appear to be ineffective to improve pikeperch reproductive performances. Therefore, their use to induce pikeperch ovulation should be put into question and the development of alternative methods is necessary to further promote pikeperch production
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Sander, Bertil [Verfasser]. "Schattenrecht der Schwarzarbeit / Bertil Sander". Frankfurt : Peter Lang GmbH, Internationaler Verlag der Wissenschaften, 2015. http://d-nb.info/1080456627/34.
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Hartard, Josefina. "Sangre altiva y noble corazón". Tesis, Universidad de Chile, 2015. http://repositorio.uchile.cl/handle/2250/130758.
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GrabadoraEn el marco del aniversario de los 90 años del Club Social y Deportivo Colo--Colo, se crearán placas homenajeando a lo que este equipo de fútbol alimenta día a día: sus hinchas, sus ídolos, su historia. Estas placas se ubicarán en aquellos lugares donde se vive y comparte el Club Social. Este proyecto nace de mi insistencia de compenetrar el estadio, y el mundo que este conlleva, con la Escuela de Arte.
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García, Herrera Gabriel. "El frío de la sangre". Universidad Peruana de Ciencias Aplicadas (UPC), 2015. http://hdl.handle.net/10757/338878.
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Ganador (Primer puesto), género Cuento. "XIX Juegos Florales 2014", realizado del 27 de Junio al 14 de Julio de 2014. Universidad Peruana de Ciencias Aplicadas - UPC. Lima, Perú.; Seudónimo: Raskolnikov
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Hellemans, Simon. "Ecology and reproduction of neotropical soil-feeding termites from the Termes group". Doctoral thesis, Universite Libre de Bruxelles, 2019. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/286072.
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The traditional view of a lifelong monogamy between a king and a queen has recently been challenged in termites. In several species, multiple parthenogenetically-produced secondary queens replace the primary queen and mate with the primary king; this strategy is referred to as “Asexual Queen Succession” (AQS). The aim of my thesis was to investigate the modalities of reproduction and the ecology of neotropical soil-feeding termites from the Termitinae, with a focus on the inquiline termite Cavitermes tuberosus in the Termes group.In the first axis, we investigated the modalities of reproduction of C. tuberosus. (i) AQS is the main reproductive strategy of this species. (ii) The evolution of AQS requires the propensity of parthenogens to develop into neotenic queens. In C. tuberosus, secondary queens develop from a developmental stage of “aspirants” which participate to the social tasks usually undertaken by workers, as long as the primary queen is alive. (iii) In AQS species, a female-biased sex ratio is expected in the dispersing reproductives. In C. tuberosus, sex ratio varies among years and according to the type of reproductives, and the population sex ratio is balanced. These results raise hints on queen-king conflict over the sex ratio.In the second axis, we described the ecology and symbioses of C. tuberosus. (iv) Wolbachia, an endosymbiotic bacterium mainly known for manipulating the reproduction of arthropods in order to enhance its own transmission, infects all individuals in societies. This bacterium, particularly abundant in a gut-associated bacteriome, may play a role in the nutrition of C. tuberosus; both partners would have evolved a mutualistic symbiosis. (v) Inquiline termites live in a nest built by other termite species and do not forage outside. Physico-chemical measures and microbiota sequencing revealed that C. tuberosus is a generalist nest-feeder.Finally, we expanded our study of the breeding systems in the phylogenetic proximity of C. tuberosus. (vi) We described Palmitermes impostor, a new genus and species as a sister-group to the genus Cavitermes. (vii) AQS is the main reproductive strategy in P. impostor, and queens of Spinitermes trispinosus and Inquilinitermes inquilinus are able to reproduce parthenogenetically. Therefore, it appears likely that the conditional use of sexual and asexual reproductions is a preadaptation common to the whole Termes group, and that it evolved into a stable element of their breeding system at least in some species.Overall, our results open new perspectives in the understanding of reproductive strategies in termites and their relationships with their bacterial symbionts.Doctorat en Sciences
info:eu-repo/semantics/nonPublished
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Weitzel, David L. "Status and ecology of walleye (Sander vitreus) and sauger (Sander canadense) in the Wabash River, Indiana". Virtual Press, 2004. http://liblink.bsu.edu/uhtbin/catkey/1286768.
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This study has presented a comprehensive overview of the status and ecology of walleye and sauger in the Wabash River, Indiana. Populations of walleye and sauger are relatively low in numbers. Both species exhibited a trend of increased abundance with river km from the mouth of the Wabash River. Summer walleye habitat use and movements were examined in the upper Wabash River. Walleye preferred run habitat over riffle or pool habitat. Lateral position, substrate use, and mean depth appeared to be influenced by discharge. Movements were generally short and occurred within a relatively small home range of 1.7 km. The population size limits the fisheries potential for walleye and sauger in the Wabash River mainstem. The upper Wabash River supports higher abundances of these species than the middle Wabash River and may provide a focal point for future management efforts.Department of Biology
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Muñoz, Analí. "Sangre vinta : gráfica inspirada en la obra de teatro SIN SANGRE, aplicada en vestuario estilo vintage". Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/101116.
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El proyecto contribuira a la validación del auto-encargo en Diseño Gráfico, como proceso para la producción de piezas de uso cotidiano. Se implementara como método de diseño: la re-construcción de lo existente, a través de la interpretación e intervención personal. A su ves reconocer la obra SIN SANGRE como hito en la escena cultural nacional y utilizarla de inspiración para el proyecto y Aportar una experiencia de aplicación de diseño gráfi¬co en soporte textil.
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Žoramskė, Inga. "UAB „“Sander Baltic“ aprūpinimo sistemos tobulinimas". Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2009~D_20091125_160233-95157.
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Įmonių praeities pasiekimai yra menkaverčiai dėl dabartinės rizikos veiksnių, rinkos nepastovumo, ekonominio neapibrėžtumo. Šiuolaikinių įmonių dominavimas rinkoje remiasi logistine sistema, gebančia prisitaikyti prie aplinkos pokyčių, gebančia balansuoti tarp „sveiko proto“ ir greito, operatyvaus reagavimo laiku į besikeičiančią pasaulinę verslo aplinką. Tai įpareigojo įmones ieškoti naujų veiklos efektyvumo didinimo būdų ir paskatino verslą atsigręžti į aprūpinimą bei aprūpinimo sistemų valdymą. Šiuo metu aprūpinimo valdymo problemų sprendimas yra vienas iš dažniausiai nagrinėjamų klausimų įmonėse ir organizacijose.
Šio darbo tikslas: išnagrinėti mokslinius šaltinius aprūpinimo tema ir išsiaiškinti aprūpinimo reikšmę šiuolaikiniam verslui. Darbe buvo atlikta UAB „Sander Baltic“ aprūpinimo sistemos analizė, norint suformuluoti ir pateikti projektinį sprendimą įmonės aprūpinimo sistemos veiklos tobulinimui.
Magistrinis darbas susideda iš trijų dalių. Pirmoje darbo dalyje yra nagrinėjama aprūpinimo reikšmė ir vaidmuo šiuolaikiniame versle. Aptarta aprūpinimo samprata, aprūpinimo sistemų taikymo ypatumai bei problemos. Antroje dalyje pateikiama UAB „Sander Baltic“ aprūpinimo sistemos analizė. Įmonės veiklos specifika yra labai svarbus aspektas numatant tolesnes aprūpinimo sistemos tobulinimo kryptis, kurios yra pateikiamos trečiojoje darbo dalyje. Pastarojoje dalyje taip pat yra analizuojami atlikto tyrimo rezultatai, vertinantys esamą aprūpinimo sistemą ir nurodantys jos... [toliau žr. visą tekstą]Achievements of the past became of low value in the context of nowadays risk factors, instability of the market, economical indeterminacy. Domination of the companies in the market is now based upon the logistics system capable of adapting to external changes, capable of balancing between rational thinking and timely rapid response to the changing environment of the global trade. This obliged the companies to search for new ways of increasing efficiency and encouraged them to spotlight the supply and supply system management. Solving the problems related to supply management is one of the biggest concerns of the companies and organizations nowadays. The goal of this work: to analyze the scientific articles dealing with the supply topic and to determine the significance of supply to the modern trade. The supply system analysis of company “Sander Baltic” Ltd. has been performed with goal to formulate and propose project solution of company’s supply system improvement. Master degree thesis consists of three parts. In the first part of the work importance and role of the supply in modern trade is analyzed. Conception of supply, peculiarities and problems of supply systems are discussed. The supply system analysis of company “Sander Baltic” Ltd. is presented in the second part of the work. Peculiarity of the company work is very important aspect in scheduling directions of supply system improvement which are presented in the third part of the work. Results of the research... [to full text]
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Sander, Charlotte [Verfasser]. "Die Zerrüttung des Arbeitsverhältnisses / Charlotte Sander". Frankfurt : Peter Lang GmbH, Internationaler Verlag der Wissenschaften, 2016. http://d-nb.info/1090773315/34.
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Young, Carlos. "Chi to Hone (Sangre y Huesos)". Anthropía, 2014. http://repositorio.pucp.edu.pe/index/handle/123456789/78034.
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El artículo analiza la película “Sangre y Huesos” sobre inmigrantes coreanos en Japón en el contexto político y económico de la II Guerra Mundial. Estos inmigrantes se ven envueltos en diferentes conflictos y en situaciones de violencia junto a redefiniciones de identidad, desencuentros generacionales y culturales dentro de una sociedad que los discrimina. Es un motivo para una reflexión antropológica que el autor exterioriza a través de alusiones continuas a la obra de José María Arguedas.
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Sander, Björn [Verfasser]. "Dignitätsbeurteilung von gastrointestinalen Stromatumoren / Björn Sander". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2021. http://d-nb.info/1238897150/34.
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Slusar, Mary Beth. "Multi-Framing in Progressive Era Women's Movements: A Comparative Analysis of the Birth Control, Temperance, and Women's Ku Klux Klan Movements". The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1269583527.
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Geiser, Madeline Allott. "The Limits of Law in the American Reproductive Freedom Movement". Ohio University Honors Tutorial College / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1587700422115124.
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Fuentes, Rivera Salcedo Teófilo José. "Mercado de sangre humana en el Perú". Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2005. https://hdl.handle.net/20.500.12672/861.
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El estudio fue realizado con el objetivo de identificar y analizar el mercado de sangre humana en el Perú, para lo cual se consideró los Bancos de sangre del Ministerio de Salud (MINSA), Seguridad Social (EsSalud), Fuerzas Armadas, Policía Nacional y Entidades privadas, durante el año 2000, evaluándose el grado de eficiencia y cobertura de atención de estos servicios de salud. Asimismo se realizó una revisión aplicativa de los criterios por la cual la sangre debería ser considerada como un bien público. Dicha investigación se realizó en función de la brecha existente entre la oferta y la demanda existente puesto que en el Perú se presenta el problema de demanda insatisfecha de sangre en forma permanente y progresiva determinado por el crecimiento poblacional y el aumento de las necesidades hospitalarias determinado por la modificación en la predominancia epidemiología del país que ha virado de enfermedades infecto-contagiosa hacia accidentes de tránsito, cánceres, enfermedades cardiovasculares, lo que genera un mayor incremento de las necesidades existente y por ende una elevación del índice de mortalidad. La incapacidad para atender oportunamente con sangre genera de por sí fatales consecuencias, como alta mortalidad por hemorragias y contaminación por transfusión, agravándose más esta situación al no establecerse el desarrollo de un Sistema Nacional de Bancos de Sangre. La hipótesis planteada fue considerar el mercado de sangre asimétrico y monopólico, lo que podría determinaría una brecha entre oferta y demanda generando ello un exceso en la demanda. Condicionada además por la ausencia de regulación de este mercado y un Sistema Nacional de Bancos de sangre que proyecte un marco de abastecimiento continuo, regular buscado un equilibrio entre la oferta y la demanda que permita integrarse con otros actores del sistema, como es el caso de la población usuaria. El método de investigación usado fue la deducción de tipo aplicada, analítica y estadístico complementariamente a las técnicas señaladas, se utilizaron información correspondiente al año 2000 y de una serie de tiempos, (series históricas), las cuales se obtuvieron de instituciones como los archivos del Programa Nacional de Hemoterapia y Bancos de sangre (PRONAHEBAS), archivos del MINSA, archivos del Programa Materno Perinatal y de encuestas de usuarios directos (pacientes) e indirectos (médicos tratantes), personal de salud, público en general. Los resultados obtenidos fueron por el lado de la demanda: es perfectamente inelástica para los Bancos de sangre del sector privado y perfectamente elástica para los bancos del sector público. Por el lado de la oferta los Bancos de sangre de Lima y Callao ofertan el 46% de la sangre de todo el país con aproximadamente un tercio de la población de la siguiente manera: Bancos del Ministerio de Salud (88) EsSalud (33) Fuerzas Armadas y Policía Nacional (6) y Privados (32), con un total de 159; el precio de las unidades de sangre oscila entre 172 y 388 nuevos soles; el movimiento económico anual de sangre en el país fue de 33´040,000 nuevos soles, siendo los Bancos de sangre del MINSA los que captan más del 50%; los intermediarios tienen un movimiento estimado de 7´030,588 nuevos soles; el 61% de los Bancos de sangre se encuentran en la Costa, mientras que el 11% se encuentra en la Selva.Presentando el mercado las siguientes características: 11,856 donaciones por cada millón de habitantes, con una cobertura de solo 29.64 por ciento de la demanda media de sangre, con capacidad de atención de solo 59% de la población nacional; La relación entre la oferta y demanda de sangre en la población peruana presentan una desigualdad marcada, por ejemplo en las zonas de alta pobreza Ayacucho (0.86%), Cajamarca (0.44%), Huancavelica (0.09%), Huánuco (0,70%), San Martín (0.64%), Amazonas (0,54%) presentan un nivel de sangre captada menor al 1%, comparado con Lima ciudad (6.9%), siendo el estándar propuesto por la Organización Panamericana de la Salud del 3%. Las conclusiones fueron: la sangre en el Perú no es un bien público porque no cumple con los criterios de imposibilidad de exclusión; la curva de demanda puede ser perfectamente elástica o inelástica dependiendo del estrato social y de la institución que la oferte.
Los Banco de sangre no son monopólicos, ya que se comportan con características similares a empresas que siguen el modelo de competencia. Existe en el país una gran una brecha entre la oferta y la demanda de sangre; ya que se oferta 311,550 unidades y se demandan 798,414, siendo la brecha de 486,864 unidades favorable para la demanda, estando ello focalizado en zonas de mayor pobreza como Ayacucho, Huancavelica, Cajamarca, Amazonas, lo que estaría condicionado muertes maternas por desabastecimiento de sangre. Sin embargo en Lima existe una brecha favorable para la oferta de 54,511 unidades, puesto que se oferta 95,964 unidades y se demanda 41,453, solo logra una cobertura del 59% de la población nacional, existiendo una mayor concentración de oferta de sangre en Lima y Callao (46% de toda la oferta de sangre de todo el país). Los Bancos de sangre no consideran los conceptos economía de mercado tales como eficiencia, costo efectividad, calidad incuestionable y lealtad total para el cliente-consumidor; las leyes y reglamentos establecen normas restrictivas en forma unilateral en contra de los usuarios. Existe libre competencia en este mercado porque los Bancos de sangre son servicios que ofertan sangre a todos estratos poblacionales, sin embargo existen barreras de entrada que condicionan un marco de desabastecimiento propiciando una brecha entre la oferta y la demanda. Los Bancos de sangre del país presentan un déficit en la oferta ocasionando una incapacidad para la atención situaciones críticas. El movimiento económico anual de los Bancos de sangre durante el año 2000 fue de 33´040,000 nuevos soles, correspondiendo a los intermediarios aproximadamente un 20% del total. El país no cuenta con Sistema Nacional de Bancos de Sangre (Centros Hemodadores Nacionales y Regionales) que regule las actividades del mercado de sangre lo que ocasiona una mayor brecha entre la oferta y la demanda.Tesis
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Sander, Jürgen H. A. [Verfasser]. "Normtatsachen im Zivilprozeß. / Jürgen H. A. Sander". Berlin : Duncker & Humblot, 2019. http://d-nb.info/1238280293/34.
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Sander, Matthias [Verfasser]. "Gemeindeeinkommensteuer - Theoretische Beurteilung und Ausgestaltungsmöglichkeiten / Matthias Sander". Aachen : Shaker, 2004. http://d-nb.info/1172615683/34.
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Sykes, Claire L. "August Sander and the task of the photographer". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ39891.pdf.
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Sander, Heiko [Verfasser]. "Pflegezeit und Familienpflegezeit aus arbeitsrechtlicher Sicht / Heiko Sander". Frankfurt a.M. : Peter Lang GmbH, Internationaler Verlag der Wissenschaften, 2018. http://d-nb.info/1161045961/34.
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Sander, Friederike [Verfasser]. "Anwendung maßgeschneiderter poröser Adsorbentien in Enzymaufreinigungsprozessen / Friederike Sander". Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2014. http://d-nb.info/1065396961/34.
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Espinosa, Anaya Nicola. "Contactados: amor y sangre en el Lote 88". Anthropía, 2014. http://repositorio.pucp.edu.pe/index/handle/123456789/77982.
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En “CONTACTADOS. Amor y sangre en el Lote 88” Marco Avilés y Daniel Silva, narran la historia de un grupo de Machiguengas en aislamiento voluntario que habita la Reserva Kugapakori Nahua Nanti. Esta Reserva Nacional comparte parte del área con el Lote 88 del que se extrae el gas de Camisea repercutiendo en la vida cotidiana de dicho grupo. Los autores buscan plasmar a través de varios artículos y un ensayo fotográico las costumbres y desafíos que afrontan los miembros de este grupo. Al mismo tiempo, al representar fotográicamente a estas personas los autores buscan evidenciar la exis- tencia de estos pueblos e incluso darles una identidad presentándolos con una foto carnet, que muchas veces son invisibilizados en el discurso político nacional que los percibe como un mero obstáculo para el desarrollo; “Y contra el petróleo han creado la igura del nativo selvático 'no conectado'; es decir, desconocido pero presumible, por lo que millones de hectáreas no deben ser exploradas y el petróleo peruano debe quedarse bajo tierra mientras se paga en el mundo US $90 por cada barril” (A. García, 2007). Así el territorio amazónico es presentado por el discurso capitalista como un área inhabitada que debe ser utilizada para fomentar el desarrollo del país, ignorando a las personas que viven ahí.
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Barrig, Maruja. "Comentarios a Razones de sangre de Gonzalo Portocarrero". Pontificia Universidad Católica del Perú, 2013. http://repositorio.pucp.edu.pe/index/handle/123456789/115402.
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Uribe, Echeverría Catalina. "La contemplación de la sangre : tres lecturas medievales". Tesis, Universidad de Chile, 2011. http://repositorio.uchile.cl/handle/2250/108735.
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La sangre ha sido universalmente considerada como la imagen fundamental de la vida, el vehículo de lo vital que, cuando se derrama, llama a la muerte. Su simbología es ambivalente, signo simultáneo de purificación y corrupción, energía y violencia. En este trabajo se busca investigar cómo la significación paradójica de la contemplación de la sangre es un motivo literario que se puede verificar a través de tres lecturas medievales: la devota cristiana, la amorosa, y la perversa. La siguiente investigación se propone establecer que la contemplación de la sangre ha sido objeto de inspiración literaria y religiosa en la Edad Media. La imagen de la sangre y/o sus gotas induce a fenómenos extáticos de ensimismamiento o contemplación que se manifiestan en formas paradójicas, y sin sentido unívoco, en diferentes períodos de la historia y a través de los siguientes registros de escritura o corpus: en el religioso y hagiográfico con las Sagradas Escrituras (entendido aquí como antecedente necesario para la comprensión de muchos fenómenos medievales) y las vidas de algunos(as) santos(as) en torno a la visión, y contemplación, de la Santa Sangre; en la primera ‘novela’ medieval Perceval El Galés o el Cuento del Grial, y los motivos literarios que giran en torno a la escena en la que el protagonista cae en un estado de contemplación profunda ante tres gotas de sangre estampadas en la nieve; y en las actas judiciales eclesiástica y civil del Proceso de Gilles de Rais de la Baja Edad Media, donde se revela el estado de éxtasis en el que caía este asesino ante el derramamiento de sangre de sus víctimas. Esta imagen simbólica tiene un efecto de desbordamiento, escapa al control y llama a una transformación en profundidad. Así como las devotas medievales pierden el sentido cuando se sienten inundadas por la sangre de Cristo, el héroe Perceval lo pierde también ante la visión hipnótica de este elemento derramado en la nieve, y Gilles de Rais representa el nivel más extremo de desenfreno y pérdida de conciencia, o sea de preponderancia –en sus palabras y en sus conductas- del inconsciente, cuando cae en éxtasis al ver el líquido vital escurriendo incansablemente de los cuerpos abiertos de niños agónicos.