Literatura académica sobre el tema "Ruminant feeding"
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Artículos de revistas sobre el tema "Ruminant feeding"
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Hartinger, Thomas y Qendrim Zebeli. "The Present Role and New Potentials of Anaerobic Fungi in Ruminant Nutrition". Journal of Fungi 7, n.º 3 (10 de marzo de 2021): 200. http://dx.doi.org/10.3390/jof7030200.
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The ruminal microbiota allows ruminants to utilize fibrous feeds and is in the limelight of ruminant nutrition research for many years. However, the overwhelming majority of investigations have focused on bacteria, whereas anaerobic fungi (AF) have been widely neglected by ruminant nutritionists. Anaerobic fungi are not only crucial fiber degraders but also important nutrient sources for the host. This review summarizes the current findings on AF and, most importantly, discusses their new application potentials in modern ruminant nutrition. Available data suggest AF can be applied as direct-fed microbials to enhance ruminal fiber degradation, which is indeed of interest for high-yielding dairy cows that often show depressed ruminal fibrolysis in response to high-grain feeding. Moreover, these microorganisms have relevance for the nutrient supply and reduction of methane emissions. However, to reach AF-related improvements in ruminal fiber breakdown and animal performance, obstacles in large-scale AF cultivation and applicable administration options need to be overcome. At feedstuff level, silage production may benefit from the application of fungal enzymes that cleave lignocellulosic structures and consequently enable higher energy exploitation from forages in the rumen. Concluding, AF hold several potentials in improving ruminant feeding and future research efforts are called for to harness these potentials.
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Nurmeiliasari, Nurmeiliasari, R. Priyanto, D. A. Astuti, Salundik y J. Takahashi. "Utilization of Rumen Mechanical Stimulator as Pseudo Fiber in Ruminant to Minimize Metabolic Problem". Indonesian Bulletin of Animal and Veterinary Sciences 27, n.º 2 (8 de enero de 2018): 67. http://dx.doi.org/10.14334/wartazoa.v27i2.1589.
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Feeding high concentrate diet, characterized with high starch and low fiber is applied to increase energy intake for an optimal production performance in ruminants. However, the prevalence of digestive disorder namely ruminal acidosis has been reported as drawback of this feeding strategy. This encourages farmers and nutritionist to supply adequate fiber in the diet to meet minimum dietary fiber requirement. However, feeding high forage diet is reported to give adverse effect on production performance, thus economic loss for farmers. Therefore, some sorts of artificial fiber utilizations have been introduced to reduce the risk of nutritional diseases as well as improving ruminant productivity. The utilization of rumen mechanical stimulator aims to maintain a healthy rumen function in ruminants fed with high concentrate diet. Objective of this article is to review recent development of rumen mechanical stimulators utilization, a pseudo fiber to substitute forage physical function in the rumen of ruminants. This review highlights the use of rumen mechanical stimulators in dairy cattle, beef cattle and sheep with particular attention in rumination time and rumen parameters. In conclusion, rumen mechanical stimulator is attributable to minimize metabolic problem associated with high concentrate feeding by providing mechanical stimulation on the rumen wall.
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Williams, P. E. V. y A. I. Frost. "Feeding the young ruminant". BSAP Occasional Publication 15 (1992): 109–18. http://dx.doi.org/10.1017/s0263967x00004134.
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AbstractYoung calves and lambs are weaned early to reduce the amount of milk products consumed and potentially improve the profitability of rearing enterprises. At weaning the pre-ruminant must make a number of adjustments to cope with the change in dietary substrate. The microbial population in the rumen must develop and metabolic changes are required in order for the young ruminant to metabolize the end products of microbial digestion. It is recognized that early weaning poses a stress to the calf but it is generally considered that the calf rapidly adjusts to the change in diet composition. A number of factors indicate that considerable advantage would be gained from delaying the weaning of calves and indeed that the calf is not physiologically capable of adapting to an early-weaning regime. Work has indicated that a diverse anaerobic microbial population can be identified in the developing rumen of the calf. More recent results show that the presence of a faculative anaerobic population, which exists for a considerable period of time, has an adverse effect on the development of a mature anaerobic population. Low rumen pH is a problem in the young calf. The level of rumen pH verges on that which would be described as acidosis in the adult ruminant. The young calf is unable to stabilize rumen pH and increasing the level of dry food intake at weaning may further depress rumen pH if the salivary buffering capacity of the calf is not adequately developed. Results indicate that the ability to utilize volatile fatty acids is not fully developed in the young calf and that early weaning can result in high levels of VFAs in the circulation.The composition of the dry diet has a major effect on the response of the calf to weaning. Choice of the ingredients can affect the buffering capacity of the rumen and the physiological development of the digestive tract. Strategies to limit the nutrient intake from dry food to levels commensurate with the developing physiology of the calf may result in a more uniform transition to full ruminant status with consequent benefits in growth rate and health.
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Bueno, Antonio Vinicius Iank, Gustavo Lazzari, Clóves Cabreira Jobim y João Luiz Pratti Daniel. "Ensiling Total Mixed Ration for Ruminants: A Review". Agronomy 10, n.º 6 (19 de junio de 2020): 879. http://dx.doi.org/10.3390/agronomy10060879.
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The interest of ensiling total mixed rations (TMR) for ruminants reemerged in the last decades. In many situations, ensiling TMR has been a sustainable alternative to efficiently handle wet byproducts in ruminant diets. An ensiled TMR typically has a markedly higher aerobic stability than its respective fresh TMR. Ensiling a TMR increases ruminal protein degradability due to proteolysis during storage. An increase of feed efficiency by ruminants fed ensiled rations have been reported, due to the improved starch digestibility in TMR silages containing cereal grains. This manuscript brings an overview of the main nutrient transformations during the ensiling of TMR and their feeding value for ruminants.
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Mir, P. S., T. A. McAllister, D. J. Gibb y E. K. Okine. "Dietary oil rich in polyunsaturated fatty acids for ruminants: Post-ruminal digesta characteristics and their implications on production". Canadian Journal of Animal Science 86, n.º 2 (1 de junio de 2006): 159–70. http://dx.doi.org/10.4141/a05-056.
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Maximizing the production of ruminants requires high intakes of feeds that are energy dense, therefore feeds that are usually high in starch. Under these conditions there can be significant passage of partially digested feed starch and fibre to the small intestine. These digesta components interact in a manner that can diminish post-ruminal digestion and absorption. However, in ruminants, the effects of digesta composition on causing physical constraints to digestion and absorption, via increased digesta viscosity have not been given adequate consideration. While elevation of digesta viscosity as a means of reducing rate of nutrient absorption and re-absorption of compounds detrimental to health is a valid approach in humans, digesta viscosity may reduce the production potential of livestock. Thus, there is merit in reviewing the feeding strategies that lead to increased viscosity and how its mitigation can be managed through inclusion of oil in ruminant diets. The interactions between digesta composition and the resultant viscosity with digestive physiology and production factors are examined. This review also highlights the associated benefits of dietary oil for ruminants, such as enhancement of bio-active lipids in ruminant products, health benefits directly to the animal, through decreasing incidence of liver abscesses and effects on production and possibly on the environment (reduction of enteropathogenic bacterial shedding and methane emissions). Key words: Ruminant, starch, digesta characteristics, viscosity, cholecystokinin
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Yanuartono, Yanuartono, Soedarmanto Indarjulianto, Alfarisa Nururrozi y Hary Purnamaningsih. "Peran Makromineral pada Reproduksi Ruminansia". Jurnal Sain Veteriner 34, n.º 2 (1 de diciembre de 2016): 155. http://dx.doi.org/10.22146/jsv.27541.
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Macromineral is one component of a nutrient that has an important role in the growth, health, production,reproduction and immune system of animals. Ruminants need makromineral such as Ca, P, Mg, K, Na , Cl and S. Mineral needs of ruminant affected by several factors such as their age, pregnancy and lactation status.Mineral deficiency can cause disturbances in reproduction ruminant. Ca and P have direct influence while Mg, K, Na, Cl and S acted indirectly on reproductive function. A complete understanding of the role macromineral on ruminant reproductive function is indispensable for the prevention of their reproductive disorders due toimproper feeding minerals.
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Dynes, R. A., D. A. Henry y D. G. Masters. "Characterising Forages for Ruminant Feeding". Asian-Australasian Journal of Animal Sciences 16, n.º 1 (1 de enero de 2003): 116–23. http://dx.doi.org/10.5713/ajas.2003.116.
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Aumaitre, A. "Feeding of non-ruminant livestock". Animal Feed Science and Technology 29, n.º 1-2 (mayo de 1990): 171–72. http://dx.doi.org/10.1016/0377-8401(90)90103-f.
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Herrera–Camacho, J., J. C. Ku-Vera, J. A. Quintal-Franco, L. G. Williams y R. Quijano-Cervera. "Effects of the addition of polyunsaturated fatty acids on rumen degradation of dry matter and neutral detergent fibre of Guinea grass (Panicum maximum) in Pelibuey sheep". Proceedings of the British Society of Animal Science 2000 (2000): 99. http://dx.doi.org/10.1017/s1752756200001009.
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Fat is often fed to sheep and cattle as a means to increase dietary energy concentration. There is increasing interest in the supplementation of ruminant diets with fats and oils. However feeding fat may reduce dry matter digestibility, and feeding ruminant animals large quantities of fat (>5% of total dry matter intake) can result in a marked negative effect on fibre and dry mater intake. The aim of the present study was to investigate the effect of fat supplementation on ruminal degradation of dry matter (DM) and neutral detergent fiber (NDF) of sheep fed a tropical hay.
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Klein, Frank, Tony Lupo, Don Pielack, Mark Mozola, David Pinero, Scott Coates, Nancy Thiex, Christoph von Holst y Jim Drouillard. "Validation Study of a Lateral-Flow Immunoassay for Detection of Ruminant By-Product Material in Animal Feeds and Feed Ingredients: Performance-Tested MethodSM 010405". Journal of AOAC INTERNATIONAL 88, n.º 6 (1 de septiembre de 2005): 1583–92. http://dx.doi.org/10.1093/jaoac/88.6.1583.
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Abstract An immunoassay with a lateral flow format has been developed for the detection of ruminant by-product material in animal feeds and feed ingredients. The test is designed for the analysis of animal feeds destined for feeding to ruminants to ensure that they do not contain ruminant by-products in violation of the ruminant-to-ruminant feed ban established by the U.S. Food and Drug Administration in 1997. This feed ban was established as a firewall against exposure of ruminant livestock animals to the prion agents responsible for neurological diseases such as bovine spongiform encephalopathy and scrapie. The test is designed for field use, e.g., at a feed mill, and yields a qualitative (presence/absence) result in 15–20 min. The objective of the study was to validate the lateral-flow test for detection of ruminant by-product material in a variety of finished animal feeds and feed ingredients. Results indicate that the test is specific for ruminant material and can detect as little as 1% ruminant material in these commodities.
Tesis sobre el tema "Ruminant feeding"
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McGill, Tyler R. "Improving the Efficiency of Dairy Cattle Feeding". Thesis, Virginia Tech, 2017. http://hdl.handle.net/10919/79951.
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Biological functions that use amino acids (AA) are limited by AA supply. This concept was likened to staves in a barrel, where the shortest stave determines the barrel's ability to hold water (Mitchell and Block, 1946). Inaccuracies in models that predict nutrient supply and requirements of dairy cows result in inefficient feeding, as under-prediction of requirements results in deficiency, and over-prediction results in excess. To avoid limitations in production due to AA deficiencies, protein is fed in quantities that likely exceed requirements. Overfeeding of AA results in increased expenses for producers and increased N excretion to the environment, providing economic and environmental incentives to increase N-efficiency. Work presented in the following chapters evaluated the impact of AA supplementation on milk production in dairy cattle, and evaluated the PREP10 and NRC (2001) nutrient requirement model predictions. In two feeding trials (Chapter 2 and Chapter 3), low protein diets did not result in decreased milk production, indicating that protein requirements were overestimated. Although supplementation of AA did not increase milk production, low protein diets resulted in greater N-efficiency, especially when supplemented with Histidine. Evaluation of the PREP10 and NRC (2001) models (Chapter 4) used production data from the literature to identify deficiencies in prediction equations, and found that correction of model bias would considerably reduce prediction errors. Model inaccuracies affect the inefficiency of dairy cow feeding, and must be evaluated to improve feed efficiency. Such improvements could act synergistically with AA supplementation to more closely match nutrient supply to requirements.Master of Science
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Osman, T. E. A. A. "Reticulo-ruminal motility and food intake in sheep". Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383451.
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Campidonico, Luca. "Plant secondary compounds in ruminant feeding: implication and effect on meat fatty acid". Doctoral thesis, Università di Catania, 2017. http://hdl.handle.net/10761/3776.
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According to FAO, about 9 billion of people will ask for food by 2050. The agriculture challenge is to increase food production by preserving food quality and natural resources.
According to WHO, red meat can favor the onset of cardiovascular disease and colon-rectal cancer; however, they are also an important source of vitamin, protein and functional fatty acids (CLA, EPA, DHA).
Future animal breeding techniques aims will be improving food nutritional profile and reducing environmental impact at the same time.
Rumen lipolysis and bio-hydrogenation allow the synthesis of a pool of fatty acids; this process is affected by animal diet. Pasture feeding increase omega-3 and CLA content in food; however, the problems related to pasture availability ask for innovative techniques that promote their synthesis even if green herbage is not present. The use of plants, or plant-extracts, containing bioactive compounds represents an achievable prospect.
The aim of this PhD was to deepen how different plants bioactive compounds affect fatty acid composition in lamb meat.
In the first experiment, the use of Trifolium pratensis (containing polyphenol oxidase enzyme, PPO) and Onobrychis viciifolia (containing condensed tannins, CT) silages were tested. The effect of PPO and CT on rumen lipid profile was comparable and even additive when silages were supplied as a mixture. In this case, meat fatty acid profile was similar to pasture-fed animals.
In the second experiment, three plants extract were added to lambs diet: hydrolizable tannins from chestnut (Castanea sativa), CT from mimosa (Acacia dealbata) and from gambier (Uncaria gambir). The effect of the different source of tannins on meat fatty acid composition was not very strong; however, multivariate analysis allowed discriminating the different groups.
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Njogah, John Njihia. "Ammoniation of low quality roughages using urea to improve their nutritive value for ruminant feeding". Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/27604.
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This study was carried out to examine the effect of urea treatment on different roughages with respect to their degradation in the rumen. The effect of urea treatment of barley straw on dry matter intake, digestibility and weight gain was also studied.
The first experiment involved urea treatment of wheat straw and orchardgrass hay. Three urea levels were used; 2, 4 and 6 g/100 g DM and the samples ensiled for 3, 6 and 9 weeks. Samples were incubated in the rumen for 1, 12, 24, 36, 48, 72 and 96 hours. Degradation constants were derived using the equation
p = a + b(1 – e[sup –ct])
where 'p' represents the amount degraded at time 't'. 'a' represents the fraction which disappears rapidly, 'b' represents that fraction which will degrade in time and the rate of degradation of this fraction is represented by the 'c'. The fraction which is undegradable in the rumen can be derived as
100 - (a + b)
. Orchardgrass hay had significantly larger degradation constants than wheat straw (P<0.05). Treatment increased the rapidly disappearing-'a' fraction of wheat straw but reduced the same fraction of orchardgrass hay (P<0.05). The 'b' fraction of orchardgrass hay was increased significantly (P<0.05) by urea treatment; in the case of wheat straw, the increase was not significant (P>0.05). Crude protein content of both materials was increased by treatment (P<0.05). Rumen disappearance of CP did not show a consistent trend especially in the case of control samples and this was an indication of microbial contamination of the residue in the bags. In other words, part of dry matter that remained in the bags was contributed by microbial material. Such material also contributed to the nitrogen content of the residue thus masking some of the crude protein disappearance that may have occurred.
In the second experiment, some of the crop by-products that are fed to ruminants in Kenya, were treated. These included barley straw, maize stover, oat straw, rice straw, wheat straw and rhodesgrass hay which was considered to be of low quality. Water was added to raise the moisture content of the materials to 20%. Urea was applied at 6 g urea/100g DM and the materials were ensiled for 6 weeks. Samples of the ensiled materials and their controls were incubated in the rumen of a cannulated steer for 1, 12, 24, 36, 48 and 72 hours. Also incubated were untreated samples of napier grass, alfalfa hay and pyrethrum marc. Rhodesgrass hay had the largest degradation constants ('a', 'b' and :a+b') before treatment and barley had the least; 13.59, 61.63, 75.22 and 7.16, 37.71, 44.87% respectively. Treatment had the largest impact on rice straw; the 'a', 'b' and 'a+b' increased from 10.24, 46.27 and 56.51% to 22.11, 70.79 and 92.91% respectively. In the case of rhodesgrass hay, treatment had no significant effect (P>0.05). Urea treatment increased significantly (P<0.05) the CP content of all materials. Analysis of data for CP disappearance indicated microbial contamination and this was more evident in the control samples where 24 and 72 hours incubation left the samples with more CP than the unincubated samples. Most of the CP in low quality roughages is bound to the lignin and is therefore undegrable. As degradation proceeds, the CP progressively becomes a larger fraction of the total dry matter which undergraded, thus there is a concentrating effect.
In the final experiment sixteen calves weighing from 86 to 176 kg were divided into four weight categories. Animals within the same weight category were randomly allocated to four different diets; barley straw treated with 4 or 6 g urea/100g DM and ensiled for six weeks; or urea added to straw at feeding time to raise the crude protein to the levels in the ensiled straws. The straws were mixed with hay in 3:1 ratio (straw:hay) and offered ad libitum. In addition each calf received 1.5 kg of concentrate plus 20 g of a mineral mixture daily. A randomized complete block design was used and each group was on diet for four weeks after which the groups were randomly re-assigned to different diets. Treatment did not improve dry matter intake significantly (P>0.05). Acid insoluble ash (AIA) was used as an internal marker to calculate apparent digestibilities for DM, CP and ADF. Straw treated with 6 g urea/100g DM and ensiled had a higher dry matter digestibility (P<0.05) than the other straws; there was a 16.3% improvement over control. Ensiling reduced CP digestibility significantly (P<0.05). Acid detergent fibre digestibility was improved in the case of straw treated with 6 g urea/100g DM and ensiled compared to the control. There were no significant differences between the different diets in terms of average daily gain but there was a trend towards higher gain as the level of urea increased.
Urea as a source of ammonia for the treatment of low quality roughages has given encouraging results and is emerging as a viable treatment method. The method has some advantages over other chemicals in that it is safer to handle, cheaper, readily available and involves simple treatment procedures.Land and Food Systems, Faculty of
Graduate
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Useni, Bilungi Alain. "Effect of exogenous fibrolytic enzymes on fibre and protein digestion in ruminant animals". Thesis, Stellenbosch : University of Stellenbosch, 2011. http://hdl.handle.net/10019.1/6744.
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Thesis (MScAgric (Animal Sciences))--University of Stellenbosch, 2011.Includes bibliography.
ENGLISH ABSTRACT: Forages are the main feed components in ruminant production systems for the reason that they are often the major source of energy available to the animal. However, only 10 to 35% of energy intake is available as net energy because the digestion of plant cell walls is not complete. This can significantly affect livestock performance and profits in production systems that use forages as a major source of nutrients of the diet. As a result of low and variable nutritive values of forage feedstuffs, attempts to improve ruminal fibre degradability have been an ongoing research topic. The use of exogenous fibrolytic enzymes (EFE) has been proposed as means to improve forage digestibility. Positive results with regard to rumen forage digestibility and other animal production traits have consequently been obtained due to increased rumen microbial activity following EFE addition in ruminant diets. Two EFE (Abo 374 and EFE 2) and one commercial yeast preparation were firstly identified and selected for their potential to improve the cumulative gas production (GP) at 24 hours of a range of feed substrates using the in vitro GP system as a screening step to identify the superior EFE products. The different feed substrates were lucerne hay, wheat straw, wheat straw treated with urea and a commercial concentrate diet. An in vitro experiment was undertaken on these four different substrates in order to evaluate the two EFE and the yeast preparation. This was to identify the most promising EFE capable of producing a significant effect on feed digestibility using organic matter digestibility (in vitro true digestibility) and fermentation characteristics (in vitro GP system). Results from the in vitro evaluation showed that EFE significantly enhanced in vitro DM degradability and GP profiles (P < 0.05). Abo 374 enzyme showed potential to increase in vitro microbial protein synthesis (MPS) of GP residues of the concentrate diet. In addition, no correlation was found between the in vitro MPS and the 48 hours cumulative GP of all the tested substrates (P < 0.05; R2 < 0.30). Treatments were found to increase in vitro MPS, feed degradability and the cumulative GP of different quality forages and the concentrate diet, with Abo 374 being the best treatment (P < 0.05). However in vitro responses of EFE were variable depending on the energy concentration and chemical composition of different substrates. Variation in MPS was mostly due to the low recovery of purine derivates with the purine laboratory analysis. On the basis of these results, Abo 374 was selected and consequently further tested in another in vitro and in situ trial using a mixed substrate of lucerne hay and wheat straw. Abo 374 significantly improved the cumulative GP, in vitro DM and NDF disappearance of the mixed substrate (P < 0.05). In addition, no correlation was found between the in vitro MPS and the cumulative GP at 48 hours (P = 0.68; R2 < 0.25). The in situ disappearance of feed nutrients (DM, NDF and CP) with Abo 374 was similar to the control. The lack of significance of disappearance was probably due to the small number of sheep used in the study and the relatively high coefficient of variation associated with measuring ruminal digestion. Abo 374 significantly increased the in situ MPS (P = 0.0088) of the mixed substrate of lucerne hay and wheat straw. Evidence of the increased MPS and both in vitro and in situ disappearance of DM and NDF resulted from the Abo 374 activity during either the pre-treatment or the digestion process. The addition of Abo 374 to the mixed substrate of lucerne hay and wheat straw appeared to have been beneficial for microbial colonization of feed particles as a result of the increased rumen activity. It could be speculated that the primary microbial colonization was thus initiated, leading to the release of digestion products that attract in return additional bacteria to the site of digestion. This EFE may be efficient to produce some beneficial depolymerisations of the surface structure of the plant material and the hydrolytic capacity of the rumen to improve microbial attachment and the feed digestibility thereafter. Therefore, the mechanism of action by which Abo 374 improved the feed digestion can be attributed to the increased microbial attachment, stimulation of the rumen microbial population and synergistic effects with hydrolases of ruminal micro-organisms. With regard to these findings, the addition of EFE in ruminant systems can improve the ruminal digestion of DM, NDF and CP to subsequently enhance the supply of the metabolizable protein to the small intestine. Key words: crude protein (CP), exogenous fibrolytic enzymes (EFE), dry matter (DM), gas production (GP), neutral detergent fibre (NDF), microbial protein synthesis (MPS).
AFRIKAANSE OPSOMMING: Ruvoere is die hoof-voerkomponent in herkouer produksiesisteme aangesien dit dikwels die vernaamstebron van energie aan herkouer is. Slegs 10 tot 35% van die energie-inname is beskikbaar as netto-enrgie, omdat die vertering van selwande onvolledig is. Dit kan die prestasie en profyt in produksiesisteme drasties beïnvloed waar ruvoere as ’n hoofbron van nutriënte in die dieet gebruik word. Aangesien die nutriëntwaarde van ruvoere laag is en baie varieer, is navorsing vir verbeterde ruminale veselvertering steeds ’n voorgesette onderwerp. Dit is voorgestel dat eksogeniese fibrolitese ensieme (EFE) gebruik kan word vir verbeterde ruvoervertering. Positiewe resultate in ruminale ruvoerverterig en ander diereproduksie-eienskappe, is verkry as gevolg van toenemende rumen mikrobiese aktiwiteit na EFE aanvulling in herkouerdiëte. Twee EFE’s (Abo 374 en EFE 2) en `n gisproduk is geïdentifiseer en geselekteer vir hul potensiaal om die kumulatiewe gasproduksie (GP) na 24 uur met ’n reeks voersubstrate te verbeter met die gebruik van die in vitro GP sisteem as seleksiemetode om die superieure EFE produkte te identifiseer. Die verskillende ruvoersubstrate was lusernhooi, koringstrooi, ureumbehandelde koringstrooi en ’n kommersiële konsentraatdieet. ’n In vitro eksperiment was onderneem om die vier verskillende substrate te gebruik om die twee EFE’s en gisproduk te evalueer. Hierdeur sou die belowendste EFE’s identifiseer kon word wat ’n betekenisvolle effek op ruvoervertering het. Die vertering van ruvoer sal bepaal word deur organiese materiaal vertering (in vitro ware vertering), asook fermentasie-eienskappe (in vitro GP sisteem). Resultate van die in vitro evaluering het getoon dat EFE’s in vitro DM degradering en GP profiele verbeter. Dit blyk dat die Abo 374 ensiem ’n potensiële toemame in in vitro mikrobiese proteïensintese (MPS), soos bepaal deur die GP oorblyfsels van konsentraat diëte, tot gevolg gehad het. Daar was geen korrelasie tussen die in vitro GP en MPS van al die proefsubstrate nie. Dit blyk dat die behandelings ’n toename in in vitro GP, MPS en ruvoerdegradeerbaarheid van lae kwaliteit ruvoer- en konsentraatdiëte gehad het, waar Abo 374 die beste behandeling was. Die in vitro reaksies van die EFE’s was egter wisselend, afhangende van die energiekonsentrasie en die chemiese samestelling van die verskillende substrate. Variasie van MPS was meestal as gevolg van die lae herwinning van purienderivate tydens die purienanalise. Op grond van dié resultate, is Abo 374 geselekteer om verdere toetse in ander in vitro en in situ proewe te doen. Die substraat wat gebruik is, was ’n 1:1 mengsel van lusernhooi en koringstrooi. Abo 374 het die kumulatiewe RP, in vitro DM en NBV verdwyning van die gemengde substraat verbeter. Boonop is geen korrelasie tussen die MPS en in vitro GP gevind nie. In situ verdwyning van DM, NBV en RP was hoër vir Abo 374, maar nie betekenisvol nie. Die gebrek aan betekenisvolle verdwynings mag die gevolg wees van die klein hoeveelheid skape wat in die proef gebruik is, asook die relatiewe hoë koëffisient van variasie wat gepaard gaan met die bepaling van ruminale vertering. Abo 374 het die in situ MPS betekenisvol verhoog. Verhoogde MPS en in vitro en in situ verdwyning van DM en NBV is waargeneemwaarskynlik as gevolg van die aktiwiteit van Abo 374 gedurende die voorafbehandeling óf die verterings proses. Die byvoeging van Abo 374 tot die gemengde substraat van lusernhooi en koringstrooi blyk om voordelig te wees vir mikrobiese kolonisering van voerpartikels as gevolg van ’n toename in rumenaktiwiteit. Die primêre mikrobiese kolonisering het waaarskynlik gelei tot die vrystelling van verteringsprodukte wat addisionele bakterieë na die plek van vertering lok. Die EFE mag geskik wees vir voordelige depolimerisasie op die oppervlakstruktuur van die plantmateriaal, asook verbeterde hidrolitiese kapasiteit van die rumen om sodoende mikrobiese aanhegting, asook ruvoervertering te verbeter. Dus, Abo 374 se meganisme van aksie wat verbeterde ruvoervertering tot gevolg het, kan toegeskryf word aan `n verhoogde mikrobiese aanhegting, stimulering van die rumen mikrobiese populasie en die sinergistiese effek met hidrolases van rumen mikroörganismes. Ten opsigte van die bevindings, kan die byvoeging van EFE in herkouersisteme ruminale vertering van DM, NBV en RP verbeter, wat dan daaropvolgend die dunderm met meer metaboliseerbare proteïn sal voorsien. Sleutelwoorde: eksogene fibrolitiese ensieme (EFE), droëmaterial (DM), ruproteïen (RP), neutraal bestande vesel (NBV), mikrobiese proteïensintese (MPS), gasproduksie (GP).
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Mullins, Chad Ryan. "Feeding behavior and metabolism of transition dairy cows supplemented with monensin". Diss., Kansas State University, 2011. http://hdl.handle.net/2097/13102.
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Doctor of PhilosophyDepartment of Animal Sciences and Industry
Barry J. Bradford
The mechanisms behind the metabolic changes observed when transition cows are administered monensin, as well as the effects of supplementing mid-lactation cows with two commercial amino acid products were investigated. Traditionally, the effects of monensin are attributed to increased gluconeogenic precursor supply, but recent research indicated that the effects of monensin extend beyond gluconeogenic flux. Thus, the primary objectives of Experiment 1 were to determine if monensin modulates transition cow feeding behavior, ruminal pH, and/or expression of key metabolic genes. Overall, monensin decreased time between meals prepartum (126 vs. 143 ± 5.0 min; P < 0.03) with a trend appearing postpartum (81.4 vs. 88.8 ± 2.9 min; P < 0.08), which could be related to the smaller ruminal pH standard deviation during the first day cows received the lactation ration (0.31 vs. 0.26 ± 0.015; P < 0.02). Monensin also increased liver mRNA abundance of carnitine palmitoyltransferase 1a (0.15 vs. 0.10 ± 0.002 arbitrary units; P < 0.04), which corresponded to a slower rate of liver triglyceride (TG) accumulation from 7 days before calving through 7 days post calving (412 vs. 128 ± 83 mg TG/g protein over this time period; P = 0.03). No significant effects of monensin supplementation were observed on other metabolic parameters or milk production. Overall, these results confirm that the effects of monensin on transition cows extend beyond altered propionate flux. In Experiment 2, mid-lactation cows consuming a control diet containing 26% wet corn gluten feed (dry matter basis) were compared to cows consuming the same diet supplemented with lysine embedded within Ca salts of fatty acids and the isopropyl ester of 2-hydroxy-4-(methylthio) butanoic acid, a methionine precursor. This trial was conducted because the NRC (2001) model indicated a lysine deficiency prior to supplementation; however amino acid supplementation had no effects. This trial was then extended to decrease dietary CP from 17.9% to 17.1%, and further increase lysine and methionine supply in the treatment diet. No production or intake effects were observed during this period, but MUN was decreased in the treated group (10.8 vs. 12.5 ± 0.2 mg/dL; P < 0.001).
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Anele, Uchenna Young [Verfasser]. "Evaluation of dual purpose cowpea varieties for dry season feeding of ruminant animals / Uchenna Young Anele". Bonn : Universitäts- und Landesbibliothek Bonn, 2011. http://d-nb.info/1016001525/34.
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Gobindram, Mohammad Noor Ehsan Newaz. "Plant secondary compounds in small ruminant feeding in stall-fed and pastoral system in the Mediterranean". Doctoral thesis, Università di Catania, 2015. http://hdl.handle.net/10761/1642.
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The utilization of alternative feed resources such as agro-industrial by-products or rangelands may contribute to reduce carbon footprint and improve sustainability of livestock farming systems.My thesis aimed at investigating how animals can be made to ingest alternative feed resources containing higher amounts of Plant Secondary compounds (PSCs) than usual forages and concentrates. PSCs are present in agroindutrial by products and in the natural vegetation of rangelands in the Mediterranean area. They are often associated with anti-nutritive properties such as reduced palatability and protein digestion but can also have positive effects especially on health. Two systems of lamb meat production were investigated, namely stall-fed in Siciliy and pastoral in Morocco.
In the stall-fed system, we investigated the potential of replacing barley by two locally available agro industrial by- products containing polyphenols, namely carob pulp and dehydrated citrus pulp (DCP). The animals were 90 days of age Ccomisana lambs assessed during fattening. Food intake pattern and blood metabolic profile were monitored. It was found that ingestion of a diet containing 35% of carob pulp or DCP resulted in comparable performance, feed efficiency and carcass weight and yield as compared to animals ingesting a cereal based diet (control). The daily feed consumption pattern was markedly affected by the inclusion of carob pulp in the diet.Control group ate more than 40% of the whole daily intake in the first 90 min of feeding compared with only 33% in the carob groups but the overall feed intake was unchanged. The highest level of DCP inclusion in the diet also produced a different rate of feed consumption, as compared to the control group. In terms of blood metabolic profile, carob ingesting animals had lower cholesterol, higher urea and higher NEFA compared to the control whilst with DCP ingestion none of the parameters measured were affectedPertaining to the blood protein profile, carob pulp addition gave minor effects, only a tendency for the albumin to globulin (AG) ratio to rise has been observed; in the case of DCP inclusion in lambs diet, the serum albumin and the AG ratio was higher compared to the control; but the values were not a a level to cause metabolic distress.
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Walker, Keitirele Patricia. "Productivity of four fodder tree species, their nutritional value and potential role in ruminant production in Eastern Botswana". Thesis, Stellenbosch : University of Stellenbosch, 2007. http://hdl.handle.net/10019.1/1421.
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Thesis (PhD (Forest and Wood Science))—University of Stellenbosch, 2007.Sustainable livestock production in semi-arid Botswana could be improved through tree planting on-farm to provide much needed protein and shade. Such action can be encouraged if the growth, productivity, nutritional value of trees and their contribution to mass gain of livestock are known.. A study at Malotwana investigated two indigenous species, Acacia galpinii and Faidherbia albida, and two exotic species, Leucaena diversifolia and L. leucocephala, at three spacings in a randomised complete block design replicated five times. The three spacings represented 400, 317 and 241 trees per hectare. The study was conducted over 6.5 years. Indigenous trees were sampled biennially and exotics annually to evaluate crown width, height, stem diameter, stem number and stem volume index. Complete plant harvesting of exotic trees at 2.5, 4.5 and 6.5 years evaluated agroforestry production. Leaves from all four species and pods of exotic species were analysed for chemical composition. Two groups of eight lambs were balanced for mass at selection for an on-farm feeding trial. Browse from exotic trees, comprising a 2:1 ratio of pods to leaves, was fed at 30% as supplement to hay to one group whose mean mass was contrasted with that of the control group fed sorghum bran at 30%. A. galpinii was a promising species, adaptable to planting away from its origin. Its crown width ranged from 5.86 m in high density plots to 6.08 m at low density and was significantly different among stands (p = 0.0406) at 6.5 years. Diameter at breast height (dbh) was significantly different among stands aged 6.5 years (p = 0.0003) and ranged between 10.38 cm at high density to 12.48 cm in low density plots, demonstrating a capacity to provide both shade and poles on-farm. At 4.5 years, F. albida attained a mean height of 4.5 m and 4.5 cm in dbh but suffered 67% mortality during a severe drought. Annual fodder production of 0.647 and 0.996 metric tonnes ha-1 for leaves and pods of L. diversifolia and 1.237 and 1.431 for L. leucocephala was recorded in years of average rainfall. Yields of 0.3 and 0.59 metric tonnes ha-1 were recorded for both species in the driest year. Equally good agroforestry production was obtained from both low and high density stands suggesting that low density plantings, which foster higher plant survival and reduce disease incidence, are best suited to the semi-arid conditions of Botswana. The crude protein of leaves ranged between 16.26 (L. diversifolia) and 25.25% (F. albida). They were highly digestible with more than twice the calcium content livestock require. Crude protein and digestibility measures were significantly different among leaves and varied significantly at different spacings (p<0.0001). Pods of the exotic species contained significantly more protein than the leaves (p<0.0001). Lambs supplemented with browse gained 102.33 g per animal per day while the control group gained 83.95 g. There were significant differences between groups during growth (p<0.05). Growing of A. galpinii, complemented with L. diversifolia and L. leucocephala, can supply short and long term feed, and greatly enhance livestock production while diversifying farm feed sources
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Gravador, Rufielyn. "Plant secondary compounds in small ruminant feeding: an alternative to synthetic compounds for improving meat quality in low-input farming systems". Doctoral thesis, Università di Catania, 2015. http://hdl.handle.net/10761/1643.
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Sheep meat is a source of polyunsaturated (PUFA) and monounsaturated (MUFA) fatty acids that confer beneficial effects to human health, but it also contains high concentration of saturated fatty acids (SFA) that are associated with the development of certain diseases. To attain a healthier intramuscular fatty acid composition, that is, meat with high PUFA to SFA ratio, and low in fatty acids n-6 to n-3 ratio, several strategies focused on animal feeding have been tested. However, a sufficient amount of antioxidants must be present in meat to counterbalance the increase in the content of readily oxidizable unsaturated fatty acids, which is vulnerable to oxidation, and from the associated deterioration of meat colour and proteins, thus antioxidants are crucial in preserving the nutritive value and extending the shelf life of the meat.
The increasing costs of feedstuffs for livestock led to the utilization of cheaper resources as alternatives to reduce the production cost. Here, locally available materials and agro-industrial by-products commonly found in the Mediterranean countries (citrus pulp, carob pulp, and olive cake) were used in feeding trials with lambs and the effects on meat quality were evaluated. These plant-derived materials contain considerable amounts of secondary bioactive metabolites, which affect the fatty acid composition and/or act as antioxidants. Hence, it was hypothesized that at appropriate levels of inclusion in to the lamb diets, these feedstuffs would not negatively affect animal health and productivity, and would represent a strategy to naturally produce a healthy and oxidatively stable meat.
In the first study (Experiment 1), Comisana male lambs were fed for 60 days:a conventional cereal-based concentrate diet, or concentrates in which 24% or 35% dried citrus pulp was included in partial replacement of barley. Following slaughter, the slices of longissimus thoracis et lomborum muscle were packed aerobically and stored for up to 6 days. The inclusion of citrus pulp in the diet effectively inhibited protein oxidation in meat by reducing protein radicals, carbonyl formation, and thiol loss in comparison to the Control. The results showed the ability of citrus pulp to be conveniently included among the ingredients of a concentrate-based diet and to result in an improved oxidative stability of meat proteins.In the second study (Experiment 2), Comisana lambs were fed for 60 days: conventional concentrates or a diet in which cereal concentrates were partially replaced by 24% or 35% carob pulp. The results demonstrated that feeding carob pulp did not affect animal productivity and improved the muscle content of PUFA, among which is rumenic acid, and reduced the SFA concentration and the n-6/n-3 PUFA ratio as compared to the control diet. Moreover, no extensive colour, lipid and protein oxidation in meat were observed over 6 days of aerobic storage. Therefore, dietary carob in lamb diet could be an effective strategy to improve the PUFA content in the meat without compromising animal growth performance and meat oxidative stability. In the third study (Experiment 3), the effect of feeding lambs with diets including linseed and olive cake on the evolution of volatile compounds (VOCs) in meat was assessed. Specifically, Appenninica lambs fed commercial concentrates or diets containing linseed, or olive cake, or combination of both. The VOC profile was determined through SPME-GC-MS analysis of raw and cooked meat The cooked meat showed higher concentrations of VOCs than raw meat and most of these were derived from fatty acid oxidation.Nevertheless, in both raw and cooked meat, none of the lipid oxidation-derived volatiles was significantly affected by the dietary treatments. Therefore, the results suggest that the replacement of cereal concentrates with linseed and/or olive cake in diets for lambs did not cause appreciable changes in VOC profile of meat.
Libros sobre el tema "Ruminant feeding"
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Jean-Claude, Blum, Wiseman Julian y Institut national de la recherche agronomique. Département de l'élevage des monogastriques., eds. Feeding of non-ruminant livestock. London: Butterworth, 1987.
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M, Chenost, Reiniger P, European Cooperation in the Field of Scientific and Technical Research (Organization), Commission of the European Communities. y Institut national de la recherche agronomique (France), eds. Evaluation of straws in ruminant feeding. London: Elsevier Applied Science, 1989.
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A, Stark Barbara, Wilkinson J. M. 1942- y Givens D. I. 1948-, eds. Ruminant feed evaluation and utilisation. Marlow: Chalcombe, 1989.
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Robert, Jarrige, ed. Ruminant nutrition: Recommended allowances and feed tables. London: Libbey, 1989.
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Forage in ruminant nutrition. San Diego: Academic Press, 1990.
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Garnsworthy, Philip C. Recent developments in ruminant nutrition 3. Nottingham: Nottingham University Press, 1996.
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Baldwin, R. L. Modeling ruminant digestion and metabolism. London: Chapman & Hall, 1995.
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Coyle, John E. In vivo digestibility studies of ruminant feed ingredients. Dublin: University College Dublin, 1996.
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Jean-Claude, Blum, Wiseman J y Institut national de la recherche agronomique (France). Département d'élevage des monogastriques., eds. Feeding of non-ruminant livestock: Collective edited work. London: Butterworths, 1987.
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Soest, Peter J. Van. Nutritional ecology of the ruminant. 2a ed. Ithaca: Comstock Pub., 1994.
Buscar texto completoCapítulos de libros sobre el tema "Ruminant feeding"
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De Boever, J. L. y S. De Campeneere. "Important Feeding Value Parameters in Ruminant Nutrition". En Breeding in a World of Scarcity, 179–86. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-28932-8_27.
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Tamminga, Seerp. "Influence of Feeding Management on Ruminant Fiber Digestibility". En Forage Cell Wall Structure and Digestibility, 571–602. Madison, WI, USA: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, 2015. http://dx.doi.org/10.2134/1993.foragecellwall.c22.
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Romanov, Victor, Alexey Mishurov y Maria Dovydenkova. "A New Probiotic Complex in Ruminant Animal Feeding". En Fundamental and Applied Scientific Research in the Development of Agriculture in the Far East (AFE-2021), 387–94. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-91405-9_42.
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Hawkey, Kerensa, John Brameld, Tim Parr, Andrew Salter y Heidi Hall. "Suitability of insects for animal feeding." En Insects as animal feed: novel ingredients for use in pet, aquaculture and livestock diets, 26–38. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789245929.0004.
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Abstract This chapter explores the nutritional composition of insects and the potential benefits and drawbacks for their inclusion into pet, poultry, pig, ruminant and aquatic animal feeds. Six species are most commonly described for use in animal feed, namely black soldier fly (BSF; Hermetia illucens) larvae and prepupae, yellow mealworm (YM; Tenebrio molitor) larvae, superworm (SW; Zophobas morio) larvae, lesser mealworm (LM; Alphitobius diaperinus) larvae, house fly (HF; Musca domestica) larvae and house crickets (HC; Acheta domesticus). The nutritional composition, variability observed due to manipulation of feed source, age, developmental stage and suitability for inclusion into animal feed are compared.
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Riley, James J., Edward P. Glenn y Carlos U. Mota. "Small ruminant feeding trials on the Arabian peninsula with Salicornia bigelovii Torr." En Halophytes as a resource for livestock and for rehabilitation of degraded lands, 273–76. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0818-8_26.
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Bustos, D. P. Jaramillo, W. B. Valderrama y A. J. Trujillo Mesa. "5. Small ruminants feeding and effect on cheese composition". En Human Health Handbooks, 73–86. The Netherlands: Wageningen Academic Publishers, 2013. http://dx.doi.org/10.3920/978-90-8686-766-0_5.
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Nefzaoui, A., H. Ben Salem y M. El Mourid. "Innovations in small ruminants feeding systems in arid Mediterranean areas". En New trends for innovation in the Mediterranean animal production, 99–116. Wageningen: Wageningen Academic Publishers, 2012. http://dx.doi.org/10.3920/978-90-8686-726-4_14.
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Cabrita, Ana Rita J., Inês M. Valente, Hugo M. Oliveira, António J. M. Fonseca y Margarida R. G. Maia. "Effects of Feeding with Seaweeds on Ruminal Fermentation and Methane Production". En Seaweeds as Plant Fertilizer, Agricultural Biostimulants and Animal Fodder, 187–210. Boca Raton, FL : CRC Press, 2019.: CRC Press, 2019. http://dx.doi.org/10.1201/9780429487156-10.
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Tesfa, Kal’ab N. y Fithawi Mehari. "Comparative Feeding Value of Halophyte as Alternative Animal Feed for Small Ruminants in Eritrea". En Sustainable Agricultural Development, 253–60. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0519-7_19.
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Noziere, P., D. Sauvant y J. L. Peyraud. "The evolution of INRA feeding systems for ruminants based on absorbed nutrients and animal responses". En Energy and protein metabolism and nutrition in sustainable animal production, 315–16. Wageningen: Wageningen Academic Publishers, 2013. http://dx.doi.org/10.3920/978-90-8686-781-3_108.
Texto completoActas de conferencias sobre el tema "Ruminant feeding"
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Matovu, Jacob y Ahmet Alçiçek. "Investigations and Concerns about the Fate of Transgenic DNA and Protein in Livestock". En International Students Science Congress. Izmir International Guest Student Association, 2021. http://dx.doi.org/10.52460/issc.2021.011.
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The fate of transgenic DNA (tDNA) and protein from feed derived from Genetically Modified organisms (GMOs) in animals has been a major issue since their commercialization in 1996. Several studies have investigated the risks of horizontal gene transfer (HGT) of tDNA and protein to bacteria or animal cells/tissues, but some of the reported data are controversial. Previous reports showed that tDNA fragments or proteins derived from GM plants could not be detected in tissues, fluids, or edible products from livestock. Other researchers have shown that there is a possibility of small fragments entering animal tissues, fluids and organs. This motivated us to update our knowledge about these concerns. Therefore, this review aimed to evaluate the probable transfer and accumulation of tDNA/proteins from transgenic feeds in animal samples (ruminant and non-ruminant) by evaluating the available experimental studies published scientifically. This study found that the tDNA/protein is not completely degraded during feed processing and digestion in Gastro-Intestinal Tract (GIT). In large ruminants (cattle), tDNA fragments/proteins were detected in GIT digesta, rumen fluid, and faeces. In small ruminants (goats), traces of tDNA/proteins were detected in GIT digesta, blood, milk, liver, kidney, heart and muscle. In pigs, they were detected in blood, spleen, liver, kidney, and GIT digesta. In poultry, traces were detected in blood, liver and GIT digesta but not in meat and eggs. Notwithstanding some studies that have shown transfer of tDNA/protein fragments in animal samples, we cannot rely on these few studies to give general evidence for transfer into tissues/fluids and organs of farm animals. However, this study clearly shows that transfer is possible. Therefore, intensive and authentic research should be conducted on GM plants before they are approved for commercial use, investigating issues such as the fate of tDNA or proteins and the effects of feeding GM feed to livestock.
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Cornale, P., A. Mimosi y L. M. Battaglini. "43. Reducing feed-food competition: impact of by-products and grazing in ruminant feeding". En EurSafe 2022. The Netherlands: Wageningen Academic Publishers, 2022. http://dx.doi.org/10.3920/978-90-8686-939-8_43.
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Ambadi, Djidda Oumar. "The Roughage Problem and Solutions in Animal Feeding in Chad". En 6th International Students Science Congress. Izmir International Guest Student Association, 2022. http://dx.doi.org/10.52460/issc.2022.003.
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Chad, a landlocked country in Central Africa with an area of 1,284,000 km², is divided into three major agro-climatic zones: the Saharan zone (47% of the area), the Sahelian zone (43% of the area), and the Sudanian zone (10% of the area). In the last census 2018, the agricultural land amounted to about 502,380 square kilometers. Agriculture accounts for two-thirds of the economy in Chad and livestock remains one of the economic backbones with cattle population being the most predominant. Pigs and chicken population are relatively small. Milk producing animals increased from 1.16 million heads in 1971 to 3.95 million head in 2020 growing at an average annual rate of 2.75%. Roughage shortage, both in quantity and quality, remains the main problem of animal production in Chad. The use of roughage in the nutrition of ruminant animals is of great physiological and economic importance. In order to produce quality roughage, it is important to rehabilitate the available meadow pasture, increase the roughage planting areas, and enhance forage conservation through silage and hay making. This review discusses the strategies to get quality and cheap roughage in order to avoid future challenges in livestock production in Chad.
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Ghorbel, Roukaya y Nedim Koşum. "Hydroponic Fodder Production: An Alternative Solution for Feed Scarcity". En 6th International Students Science Congress. Izmir International Guest Student Association, 2022. http://dx.doi.org/10.52460/issc.2022.005.
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Feeds and animal nutrition presents a major sector in providing food security. However, there is a large gap between fodder supply and demand [1]. This gap can be attributed basically to climatic changes, urbanization and increase in meat demand. Nowadays, especially after the covid-19 pandemic, there is a crucial problem in supplying fresh green feed to remote and urban regions. Hydroponic fodder is an alternative solution to provide the sustainability of quality forage for ruminant. Hydroponics can include several crops such as maize, wheat and barley, the fodder can be produced in a short duration (6-10 days) and all year around. The hydroponics fodder present various benefits for animal health. Feeding hydroponically produced fodder increases the digestibility of the nutrients in the ration, which could increase milk production [2]. Hydroponic fodder production is an economic solution particularly where the conventional production of green fodder is limited or unavailable. This technology put forward a solution to address the shortage of forage production caused by the scarcity of green feed in dry seasons and urban areas [3]. That´s why, further studies and academic efforts are needed to expand hydroponic fodder production. The purpose of this study is to review the actual literature on hydroponic fodder production process, nutritious value and effects on livestock production and productivity.
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Ünlü, Elif Işılay y Ahmet Çınar. "Lesion Detection on Skin Images Using Improved U-Net". En International Students Science Congress. Izmir International Guest Student Association, 2021. http://dx.doi.org/10.52460/issc.2021.022.
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The fate of transgenic DNA (tDNA) and protein of feeds from Genetically Modified organisms (GMOs) in animals has been an important topic since their commercialization in 1996. Several studies have investigated about risks of horizontal gene transfer (HGT) of tDNA and proteins to bacteria or animal cells/tissues, however, the reported data is at times controversial. Earlier reports showed that tDNA fragments or protein derived from GM plants have not been detected in tissues, fluids, or edible products of farm animals. Other researchers have come out to demonstrate that there is the possibility of small fragments leaking out into the animal tissues, fluids and organs. This motivated us to update our knowledge about these concerns. Therefore, this review aimed at assessing the likely transfer and accumulation of tDNA/ proteins from transgenic feeds to animal (ruminants and non-ruminants) samples through evaluating the available experimental scientific published studies. This study has found out that the tDNA or protein is not completely degraded during feed processing and digestion in the Gastro-Intestinal Tract (GIT). In large ruminants (Cattle), tDNA fragments/protein have been detected in the GIT digesta, ruminal fluid and feces. In small ruminants (Goats), traces of tDNA/proteins have been detected in the GIT digesta, blood, milk, liver, kidney, heart and muscle. In pigs, they have been detected in blood, spleen, liver kidney and in the GIT digesta. In poultry, traces have been seen in blood, liver and GIT digesta but not in meat and Eggs. Regardless of some studies that have shown the transfer of tDNA/protein fragments to animal samples, we cannot base on these few studies to give a piece of general evidence about their transfer into tissues/fluids and organs of livestock animals. However, this study clearly shows possible transfer, hence intensive and authentic research on GM crops should be done before they are allowed for commercial use, studying issues like the fate of tDNA or proteins and the effect of feeding GM feeds to livestock.
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ISMAILOV, Ismail Sagidovich, Nina Vladimirovna TREGUBOVA, Rashid Hasanbievich KOCHKAROV, Anna Victorovna MORGUNOVA y Natalija Alecseevna DRIZHD. "THE RELATIONSHEEP OF AMINO ACID METABOLISM WITH PRODUCTIVITY OF GROWING YOUNG SHEEP". En RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.125.
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A number of studies on digestion of the ruminants have shown the process of synthesis of all the essential amino acids in the rumen (Abu Fadel, 2004; Trukhachev, V., Zlidnev, N. 2008). However, according to some researches, microbial protein is deficient in methionine and lysine. This assumption also proves the need for further study of the influence of balanced amino acids to these diets on the growth, development and productive performance of the ruminants. Scientific approbation of the issues related to establishing the requirements of young sheep in lysine and methionine with cystine and development of recommendations for their proper balance in animal rations have been conducted along with a series of physiological, scientific and practical experiments. For this purpose, we used the following research methods: preparative, analytical, measuring and calculating. Zootechnical evaluation of young sheep’s diet with different levels of lysine and methionine with cysteine has been conducted, and influence of these components on growth, development, metabolism, the use of amino acids and wool productivity have been studied. The importance of studying the content of amino acids in plasma is evident because they are the indicators of protein metabolism in the animals’ organism and represent themselves the exchange fund when used in the biochemical transformations in the process of updating the protein of the body tissues and the synthesis of animal products. Increase in young sheep’s diet of lysine and methionine can improve their productivity, reduce the cost of feed energy and improve biochemical indexes of meat, taking into account the optimization of its amino acid composition. Efficiency increase of young sheep during the process of the individual development is linked to conditions of feeding and in particular the usefulness of a protein food, which is primarily determined by sufficient intake of essential amino acids - lysine and methionine with cystine. Thus, the inclusion in the diet of growing young sheep some amount of synthetic amino acids – 6–8 grams of lysine and 3–4 g of methionine per 1 fodder unit, enhances oxidation-reduction processes, increase productivity, i.e. wool yield and other indicators. The content of free amino acids in plasma directly depends on their availability in feed. The use of synthetic amino acids (lysine, methionine and cystine) contributed to the increase of their content in a free state in the blood serum, indicating their increased demand in sheep. With the use of different doses of synthetic amino acids (lysine, methionine, cystine) in the diets of young sheep it has been found that the organization of optimum conditions of feeding, care and management of sheep in winter season help to avoid abrupt changes in the structure of the skin, to ensure the normal development of the histological structure and morphogenetic processes.)
Informes sobre el tema "Ruminant feeding"
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Gottlieb, Yuval y Bradley A. Mullens. Might Bacterial Symbionts Influence Vectorial Capacity of Biting Midges for Ruminant Viruses? United States Department of Agriculture, septiembre de 2010. http://dx.doi.org/10.32747/2010.7699837.bard.
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- Original objectives and revision: The feasibility study performed in the last year was aimed at determining the symbiotic profiles of eight selected Culicoidesspecies in Israel and the USA by: Comparing bacterial communities among geographic populations of primary bluetongue virus (BTV) vectors. Comparing bacterial communities between adults of field-collected, mammal-feeding BTV vectors and non-vectors. Comparing bacterial communities within and between mammal feeders and bird feeders, with special attention to species with unique immature habitats. We made an effort to collect the eight species during the beginning of the project, however, due to the short available collection season, and the significant changes in habitats available for Israeli Culicoides, we initially determined the symbiotic profile of five species: two BTV vectors (C. sonorensis, C. imicola), one mammal feeders with unknown vectoring ability (C. schultzei), one bird feeder (C. crepuscularis), and one unique habitat species (C. cacticola). In addition, upon preliminary symbiont identification we focused our effort on relevant specific symbionts. Background: Biting midges (Culicoides, Diptera: Ceratopogonidae) are vectors of many major viral diseases affecting farm animals, including BT, which is listed among the most damaging by the World Organization for Animal Health (OIE) and has recently emerged in completely unexpected areas (Northern Europe). One of the strategies to reduce the vectorial capacity of insect vectors is by manipulating their specific symbionts either to affect the vector species or to influence performance of the disease agent within it. Despite significant efforts to elucidate the vectorial capacity of certain Culicoidesspecies, and the critical basis of variability in infection, almost no attention has been given to symbiotic interactions between the vector and its bacterial tenants. It is now established that bacterial symbionts have major influences on their host biology, and may interact with disease agents vectored by their hosts. - Major conclusions, solutions, achievements: During the feasibility project we have found two major bacterial symbionts in Israeli and American Culicoides. In Israel we discovered that C. imicola, a known vector of BT, and C. schultzeigp. a suspected vector of BT, carry the symbiotic bacterium Cardinium, a reproductive manipulator symbiont. In C. imicolathe infection rate was close to 50%, and in C. schultzeiit was lower, and restricted to one of two species within Schultzeigroup. In 3 American species (C. sonorensis, C. crepuscularis, C. cacticola) we found the bacterium Burkholderiasp. In all species tested we have also found other bacterial species in diverse quantities and frequencies. - Implications, both scientific and agricultural: Finding specific symbionts in Culicoidesvector species is the first step in developing symbiont based control (SBC) strategies. Both identified symbionts are known from other insects, and Cardiniumis also known as a reproductive manipulator that can cause cytoplasmic incompatibility, an important phenomenon that can be used for spreading desired traits in infected populations. The role of the symbionts in Culicoideshost can be target for manipulation to reduce the vectorial capacity of the host by either changing its fitness so that it is unable to serve as a vector, or by directly changing the symbiont in a way that will affect the performance of the disease agent in its vector. Since Burkholderiaperhaps can be cultured independently of the host, it is a promising candidate for the later option. Thus, we have now opened the door for studying the specific interactions between symbionts and vector species.
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Weinberg, Zwi G., Adegbola Adesogan, Itzhak Mizrahi, Shlomo Sela, Kwnag Jeong y Diwakar Vyas. effect of selected lactic acid bacteria on the microbial composition and on the survival of pathogens in the rumen in context with their probiotic effects on ruminants. United States Department of Agriculture, enero de 2014. http://dx.doi.org/10.32747/2014.7598162.bard.
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This research project was performed in context of the apparent probiotic effect of selected lactic acid bacteria (LAB) silage inoculants on the performance of ruminants (improved feed intake, faster live-weight gain, higher milk yields and improved feed efficiency). The overall objective was to find out how LAB affect ruminant performance. The project included several “chapters” as follows: 1. The effect of LAB silage inoculants on the survival of detrimental bacteria in rumen fluid, in vitro study (Weinberg et al., The Volcani Center). An in vitro model was developed to study the interaction between selected LAB and an E. coli strain tagged with green fluorescence protein (GFP) in buffered RF. Results indicated that both LAB inoculants and E. coli survived in the RF for several days; both LAB inoculants and LAB-treated silages did not affect survival of E. coli in rumen fluid in vitro. The effect of feeding baled wheat silages treated with or without three selected LAB silage inoculants on the performance of high-lactating cows (Weinberg et al., The Volcani Center). Treatments included control (no additive), Lacobacillusbuchneri40788 (LB), Lactobacillus plantarumMTD1 40027 (LP) and Pediococcuspentosaceus30168 (PP), each applied at 10⁶ cfu/g FM. The silages were included in the TMR of 32 high milking Holstein cows in a controlled feeding experiment. All baled silages were of good quality. The LB silage had the numerically highest acetic acid and were the most stable upon aerobic exposure. The cows fed the LB silages had the highest daily milk yields, percent milk fat and protein. The microbiome of baled wheat silages and changes during ensiling of wheat and corn (Sela et al., The Volcani Center). Bacterial community of the baled silages was dominated mainly of two genera in total, dominated by Lactobacillus and Clostridium_sensu_stricto_12 with 300 other genera at very low abundance. Fungal community was composed mainly of two genera in total, dominated by Candida and Monascuswith 20 other genera at very low abundance. In addition, changes in the microbiome during ensiling of wheat and corn with and without addition of L. plantarumMTD1 was studied in mini-silos. Overall 236 bacterial genera were identified in the fresh corn but after 3 months Lactobacillus outnumbered all other species by acquiring 95% of relative abundance. The wheat silage samples are still under analysis. The effect of applying LAB inoculants at ensiling on survival of E. coli O157:H7 in alfalfa and corn silages(Adesogan et al., University of Florida). E. coli (10⁵ cfu/g) was applied to fresh alfalfa and corn at ensiling with or without L. plantarumor L. buchneri. The pathogen was added again after about 3 moths at the beginning of an aerobic exposure period. The inoculants resulted in faster decrease in pH as compared with the control (no additives) or E. coli alone and therefore, the pathogen was eliminated faster from these silages. After aerobic exposure the pathogen was not detected in the LAB treated silages, whereas it was still present in the E. coli alone samples. 5. The effect of feeding corn silage treated with or without L. buchnerion shedding of E. coli O157:H7 by dairy cows (Adesogan et al., UFL). BARD Report - Project 4704 Page 2 of 12 Five hundred cows from the dairy herd of the University of Florida were screened for E. coli shedding, out of which 14 low and 13 high shedders were selected. These cows were fed a total mixed ration (TMR) which was inoculated with E. coli O157:H7 for 21 days. The TMR included corn silage treated with or without L. buchneri. The inoculated silages were more stable upon aerobic exposure than the control silages; the silage inoculant had no significant effect on any milk or cow blood parameters. However, the silage inoculant tended to reduce shedding of E. coli regardless of high or low shedders (p = 0.06). 6. The effect of feeding baled wheat silages treated with or without three selected LAB silage inoculants on the rumen microbiome (Mizrahi et al., BGU). Rumen fluid was sampled throughout the feeding experiment in which inoculated wheat silages were included in the rations. Microbial DNA was subsequently purified from each sample and the 16S rRNA was sequenced, thus obtaining an overview of the microbiome and its dynamic changes for each experimental treatment. We observed an increase in OTU richness in the group which received the baled silage inoculated with Lactobacillus Plantarum(LP). In contrast the group fed Lactobacillus buchneri(LB) inoculated silage resulted in a significant decrease in richness. Lower OTU richness was recently associated in lactating cows with higher performance (Ben Shabatet al., 2016). No significant clustering could be observed between the different inoculation treatments and the control in non metric multi-dimentional scaling, suggesting that the effect of the treatments is not the result of an overall modulation of the microbiome composition but possibly the result of more discrete interactions. Significant phylum level changes in composition also indicates that no broad changes in taxa identity and composition occurred under any treatment A more discrete modulation could be observed in the fold change of several taxonomic groups (genus level analysis), unique to each treatment, before and after the treatment. Of particular interest is the LB treated group, in which several taxa significantly decreased in abundance. BARD Report - Project 4704 Page 3 of 12
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Brosh, Arieh, David Robertshaw, Yoav Aharoni, Zvi Holzer, Mario Gutman y Amichai Arieli. Estimation of Energy Expenditure of Free Living and Growing Domesticated Ruminants by Heart Rate Measurement. United States Department of Agriculture, abril de 2002. http://dx.doi.org/10.32747/2002.7580685.bard.
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Research objectives were: 1) To study the effect of diet energy density, level of exercise, thermal conditions and reproductive state on cardiovascular function as it relates to oxygen (O2) mobilization. 2) To validate the use of heart rate (HR) to predict energy expenditure (EE) of ruminants, by measuring and calculating the energy balance components at different productive and reproductive states. 3) To validate the use of HR to identify changes in the metabolizable energy (ME) and ME intake (MEI) of grazing ruminants. Background: The development of an effective method for the measurement of EE is essential for understanding the management of both grazing and confined feedlot animals. The use of HR as a method of estimating EE in free-ranging large ruminants has been limited by the availability of suitable field monitoring equipment and by the absence of empirical understanding of the relationship between cardiac function and metabolic rate. Recent developments in microelectronics provide a good opportunity to use small HR devices to monitor free-range animals. The estimation of O2 uptake (VO2) of animals from their HR has to be based upon a consistent relationship between HR and VO2. The question as to whether, or to what extent, feeding level, environmental conditions and reproductive state affect such a relationship is still unanswered. Studies on the basic physiology of O2 mobilization (in USA) and field and feedlot-based investigations (in Israel) covered a , variety of conditions in order to investigate the possibilities of using HR to estimate EE. In USA the physiological studies conducted using animals with implanted flow probes, show that: I) although stroke volume decreases during intense exercise, VO2 per one heart beat per kgBW0.75 (O2 Pulse, O2P) actually increases and measurement of EE by HR and constant O2P may underestimate VO2unless the slope of the regression relating to heart rate and VO2 is also determined, 2) alterations in VO2 associated with the level of feeding and the effects of feeding itself have no effect on O2P, 3) both pregnancy and lactation may increase blood volume, especially lactation; but they have no effect on O2P, 4) ambient temperature in the range of 15 to 25°C in the resting animal has no effect on O2P, and 5) severe heat stress, induced by exercise, elevates body temperature to a sufficient extent that 14% of cardiac output may be required to dissipate the heat generated by exercise rather than for O2 transport. However, this is an unusual situation and its affect on EE estimation in a freely grazing animal, especially when heart rate is monitored over several days, is minor. In Israel three experiments were carried out in the hot summer to define changes in O2P attributable to changes in the time of day or In the heat load. The animals used were lambs and young calves in the growing phase and highly yielding dairy cows. In the growing animals the time of day, or the heat load, affected HR and VO2, but had no effect on O2P. On the other hand, the O2P measured in lactating cows was affected by the heat load; this is similar to the finding in the USA study of sheep. Energy balance trials were conducted to compare MEI recovery by the retained energy (RE) and by EE as measured by HR and O2P. The trial hypothesis was that if HR reliably estimated EE, the MEI proportion to (EE+RE) would not be significantly different from 1.0. Beef cows along a year of their reproductive cycle and growing lambs were used. The MEI recoveries of both trials were not significantly different from 1.0, 1.062+0.026 and 0.957+0.024 respectively. The cows' reproductive state did not affect the O2P, which is similar to the finding in the USA study. Pasture ME content and animal variables such as HR, VO2, O2P and EE of cows on grazing and in confinement were measured throughout three years under twenty-nine combinations of herbage quality and cows' reproductive state. In twelve grazing states, individual faecal output (FO) was measured and MEI was calculated. Regression analyses of the EE and RE dependent on MEI were highly significant (P<0.001). The predicted values of EE at zero intake (78 kcal/kgBW0.75), were similar to those estimated by NRC (1984). The EE at maintenance condition of the grazing cows (EE=MEI, 125 kcal/kgBW0.75) which are in the range of 96.1 to 125.5 as presented by NRC (1996 pp 6-7) for beef cows. Average daily HR and EE were significantly increased by lactation, P<0.001 and P<0.02 respectively. Grazing ME significantly increased HR and EE, P<0.001 and P<0.00l respectively. In contradiction to the finding in confined ewes and cows, the O2P of the grazing cows was significantly affected by the combined treatments (P<0.00l ); this effect was significantly related to the diet ME (P<0.00l ) and consequently to the MEI (P<0.03). Grazing significantly increased O2P compared to confinement. So, when EE of grazing animals during a certain season of the year is estimated using the HR method, the O2P must be re measured whenever grazing ME changes. A high correlation (R2>0.96) of group average EE and of HR dependency on MEI was also found in confined cows, which were fed six different diets and in growing lambs on three diets. In conclusion, the studies conducted in USA and in Israel investigated in depth the physiological mechanisms of cardiovascular and O2 mobilization, and went on to investigate a wide variety of ruminant species, ages, reproductive states, diets ME, time of intake and time of day, and compared these variables under grazing and confinement conditions. From these combined studies we can conclude that EE can be determined from HR measurements during several days, multiplied by O2P measured over a short period of time (10-15 min). The study showed that RE could be determined during the growing phase without slaughtering. In the near future the development microelectronic devices will enable wide use of the HR method to determine EE and energy balance. It will open new scopes of physiological and agricultural research with minimizes strain on animals. The method also has a high potential as a tool for herd management.
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Brosh, Arieh, Gordon Carstens, Kristen Johnson, Ariel Shabtay, Joshuah Miron, Yoav Aharoni, Luis Tedeschi y Ilan Halachmi. Enhancing Sustainability of Cattle Production Systems through Discovery of Biomarkers for Feed Efficiency. United States Department of Agriculture, julio de 2011. http://dx.doi.org/10.32747/2011.7592644.bard.
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Feed inputs represent the largest variable cost of producing meat and milk from ruminant animals. Thus, strategies that improve the efficiency of feed utilization are needed to improve the global competitiveness of Israeli and U.S. cattle industries, and mitigate their environmental impact through reductions in nutrient excretions and greenhouse gas emissions. Implementation of innovative technologies that will enhance genetic merit for feed efficiency is arguably one of the most cost-effective strategies to meet future demands for animal-protein foods in an environmentally sustainable manner. While considerable genetic variation in feed efficiency exist within cattle populations, the expense of measuring individual-animal feed intake has precluded implementation of selection programs that target this trait. Residual feed intake (RFI) is a trait that quantifies between-animal variation in feed intake beyond that expected to meet energy requirements for maintenance and production, with efficient animals being those that eat less than expected for a given size and level of production. There remains a critical need to understand the biological drivers for genetic variation in RFI to facilitate development of effective selection programs in the future. Therefore, the aim of this project was to determine the biological basis for phenotypic variation in RFI of growing and lactating cattle, and discover metabolic biomarkers of RFI for early and more cost-effective selection of cattle for feed efficiency. Objectives were to: (1) Characterize the phenotypic relationships between RFI and production traits (growth or lactation), (2) Quantify inter-animal variation in residual HP, (3) Determine if divergent RFIphenotypes differ in HP, residual HP, recovered energy and digestibility, and (4) Determine if divergent RFI phenotypes differ in physical activity, feeding behavior traits, serum hormones and metabolites and hepatic mitochondrial traits. The major research findings from this project to date include: In lactating dairy cattle, substantial phenotypic variation in RFI was demonstrated as cows classified as having low RMEI consumed 17% less MEI than high-RMEI cows despite having similar body size and lactation productivity. Further, between-animal variation in RMEI was found to moderately associated with differences in RHP demonstrating that maintenance energy requirements contribute to observed differences in RFI. Quantifying energetic efficiency of dairy cows using RHP revealed that substantial changes occur as week of lactation advances—thus it will be critical to measure RMEI at a standardized stage of lactation. Finally, to determine RMEI in lactating dairy cows, individual DMI and production data should be collected for a minimum of 6 wk. We demonstrated that a favorably association exists between RFI in growing heifers and efficiency of forage utilization in pregnant cows. Therefore, results indicate that female progeny from parents selected for low RFI during postweaning development will also be efficient as mature females, which has positive implications for both dairy and beef cattle industries. Results from the beef cattle studies further extend our knowledge regarding the biological drivers of phenotypic variation in RFI of growing animals, and demonstrate that significant differences in feeding behavioral patterns, digestibility and heart rate exist between animals with divergent RFI. Feeding behavior traits may be an effective biomarker trait for RFI in beef and dairy cattle. There are differences in mitochondrial acceptor control and respiratory control ratios between calves with divergent RFI suggesting that variation in mitochondrial metabolism may be visible at the genome level. Multiple genes associated with mitochondrial energy processes are altered by RFI phenotype and some of these genes are associated with mitochondrial energy expenditure and major cellular pathways involved in regulation of immune responses and energy metabolism.
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Weinberg, Zwi G., Richard E. Muck, Nathan Gollop, Gilad Ashbell, Paul J. Weimer y Limin Kung, Jr. effect of lactic acid bacteria silage inoculants on the ruminal ecosystem, fiber digestibility and animal performance. United States Department of Agriculture, septiembre de 2003. http://dx.doi.org/10.32747/2003.7587222.bard.
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The overall objective of the whole research was to elucidate the mechanisms by which LAB silage inoculants enhance ruminant performance. The results generated will permit the development of better silage inoculants that maximize both silage preservation and animal performance. For this one-year BARD feasibility study, the objectives were to: 1. determine whether lactic acid bacteria (LAB) used in inoculants for silage can survive in rumen fluid (RF) 2.select the inoculants that survived best, and 3. test whether LAB silage inoculants produce bacteriocins-like substances. The most promising strains will be used in the next steps of the research. Silage inoculants containing LAB are used in order to improve forage preservation efficiency. In addition, silage inoculants enhance animal performance in many cases. This includes improvements in feed intake, liveweight gain and milk production in 25-40% of studies reviewed. The cause for the improvement in animal performance is not clear but appears to be other than direct effect of LAB inoculants on silage fermentation. Results from various studies suggest a possible probiotic effect. Our hypothesis is that specific LAB strains interact with rumen microorganisms which results in enhanced rumen functionality and animal performance. The first step of the research is to determine whether LAB of silage inoculants survive in RF. Silage inoculants (12 in the U.S. and 10 in Israel) were added to clarified and strained RF. Inoculation rate was 10 ⁶ (clarified RF), 10⁷ (strained RF) (in the U.S.) and 10⁷, 10⁸ CFU ml⁻¹ in Israel (strained RF). The inoculated RF was incubated for 72 and 96 h at 39°C, with and without 5 g 1⁻¹ glucose. Changes in pH, LAB numbers and fermentation products were monitored throughout the incubation period. The results indicated that LAB silage inoculants can survive in RF. The inoculants with the highest counts after 72 h incubation in rumen fluid were Lactobacillus plantarum MTD1 and a L. plantarum/P. cerevisiae mixture (USA) and Enterococcus faecium strains and Lactobacillus buchneri (Israel). Incubation of rumen fluid with silage LAB inoculants resulted in higher pH values in most cases as compared with that of un-inoculated controls. The magnitude of the effect varied among inoculants and typically was enhanced with the inoculants that survived best. This might suggest the mode of action of LAB silage inoculants in the rumen as higher pH enhances fibrolytic microorganisms in the rumen. Volatile fatty acid (VFA) concentrations in the inoculated RF tended to be lower than in the control RF after incubation. However, L. plalltarull1 MTDI resulted in the highest concentrations of VFA in the RF relative to other inoculants. The implication of this result is not as yet clear. In previous research by others, feeding silages which were inoculated with this strain consistently enhanced animal performance. These finding were recently published in Weinberg et.al.. (2003), J. of Applied Microbiology 94:1066-1071 and in Weinberg et al.. (2003), Applied Biochemistry and Biotechnology (accepted). In addition, some strains in our studies have shown bacteriocins like activity. These included Pediococcus pentosaceus, Enterococcus faecium and Lactobacillus plantarum Mill 1. These results will enable us to continue the research with the LAB strains that survived best in the rumen fluid and have the highest potential to affect the rumen environment.
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Varga, Gabriella A., Amichai Arieli, Lawrence D. Muller, Haim Tagari, Israel Bruckental y Yair Aharoni. Effect of Rumen Available Protein, Amimo Acids and Carbohydrates on Microbial Protein Synthesis, Amino Acid Flow and Performance of High Yielding Cows. United States Department of Agriculture, agosto de 1993. http://dx.doi.org/10.32747/1993.7568103.bard.
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The effect of rumen available protein amino acids and carbohydrates on microbial protein synthesis, amino acid flow and performance of high yielding dairy cows was studied. A significant relationship between the effective degradabilities of OM in feedstuffs and the in vivo ruminal OM degradation of diets of dairy cows was found. The in situ method enabled the prediction of ruminal nutrients degradability response to processing of energy and nitragenous supplements. The AA profile of the rumen undegradable protein was modified by the processing method. In a continuous culture study total N and postruminal AA flows, and bacterial efficiency, is maximal at rumen degradable levels of 65% of the CP. Responses to rumen degradable non carbohydrate (NSC) were linear up to at least 27% of DM. Higher CP flow in the abomasum was found for cows fed high ruminally degradable OM and low ruminally degradable CP diet. It appeared that in dairy cows diets, the ratio of rumen degradable OM to rumenally degradable CP should be at least 5:1 in order to maximize postruminal CP flow. The efficiency of microbial CP synthesis was higher for diets supplemented with 33% of rumen undegradable protein, with greater amounts of bacterial AA reaching the abomasum. Increase in ruminal carbohydrate availability by using high moisture corn increased proportions of propionate, postruminal nutrients flow, postruminal starch digestibility, ruminal availability of NSC, uptake of energy substrates by the mammory gland. These modifications resulted with improvement in the utilization of nonessential AA for milk protein synthesis, in higher milk protein yield. Higher postruminal NSC digestibility and higher efficiency of milk protein production were recorded in cows fed extruded corn. Increasing feeding frequency increased flow of N from the rumen to the blood, reduced diurnal variation in ruminal and ammonia, and of plasma urea and improved postruminal NSC and CIP digestibility and total tract digestibilities. Milk and constituent yield increased with more frequent feeding. In a study performed in a commercial dairy herd, changes in energy and nitrogenous substrates level suggested that increasing feeding frequency may improve dietary nitrogen utilization and may shift metabolism toward more glucogenesis. It was concluded that efficiency of milk protein yield in high producing cows might be improved by an optimization of ruminal and post-ruminal supplies of energy and nitrogenous substrates. Such an optimization can be achieved by processing of energy and nitrogenous feedstuffs, and by increasing feeding frequency. In situ data may provide means for elucidation of the optimal processing conditions.
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Huber, John Tal, Joshuah Miron, Brent Theurer, Israel Bruckental y Spencer Swingle. Influence of Ruminal Starch Degradability on Performance of High Producing Dairy Cows. United States Department of Agriculture, enero de 1994. http://dx.doi.org/10.32747/1994.7568748.bard.
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This research project entitled "Influence of Ruminal Starch Degradability on Performance of High Producing Dairy Cows" had the following objectives: a) Determine effects of feeding varying amounts of ruminally degradable starch (RDS) on efficiency of milk and milk protein production; and 2) Investigate digestive and metabolic mechanisms relating to lactation responses to diets varying in ruminal and total starch degradability. Four lactation studies with high producing cows were conducted in which steam-flaked (~ 75% RDS) was compared with dry-rolled sorghum (~ 50% RDS) grain. All studies demonstrated increased efficiency of conversion of feed to milk (FCM/DMI) and milk protein as amount of RDS in the diet increased by feeding steam-flaked sorghum. As RDS in diets increased, either by increased steam-flaked sorghum, grinding of sorghum, or increasing the proportion of wheat to sorghum, so also did ruminal and total tract digestibilities of starch and neutral-detergent soluble (NDS) carbohydrate. Despite other research by these two groups of workers showing increased non-ammonia N (NAN) flowing from the rumen to the duodenum with higher RDS, only one of the present studies showed such an effect. Post-absorptive studies showed that higher dietary RDS resulted in greater urea recycling, more propionate absorption, a tendency for greater output of glucose by the liver, and increased uptake of alpha-amino nitrogen by the mammary gland. These studies have shown that processing sorghum grain through steam-flaking increases RDS and results in greater yields and efficiency of production of milk and milk protein in high producing dairy cows.
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Osman, Mohamed, Judith Stabel, Ken Onda, Scot Down, Wanda Kreikemeier, Douglas Ware y Donald C. Beitz. Modification of Digestive System Microbiome of Lactating Dairy Cows by Feeding Bovamine®: Effect on Ruminal Fermentation. Ames (Iowa): Iowa State University, enero de 2012. http://dx.doi.org/10.31274/ans_air-180814-884.
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Klement, Eyal, Elizabeth Howerth, William C. Wilson, David Stallknecht, Danny Mead, Hagai Yadin, Itamar Lensky y Nadav Galon. Exploration of the Epidemiology of a Newly Emerging Cattle-Epizootic Hemorrhagic Disease Virus in Israel. United States Department of Agriculture, enero de 2012. http://dx.doi.org/10.32747/2012.7697118.bard.
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In September 2006 an outbreak of 'Bluetongue like' disease struck the cattle herds in Israel. Over 100 dairy and beef cattle herds were affected. Epizootic hemorrhagic disease virus (EHDV) (an Orbivirusclosely related to bluetongue virus (BTV)), was isolated from samples collected from several herds during the outbreaks. Following are the aims of the study and summary of the results: which up until now were published in 6 articles in peer-reviewed journals. Three more articles are still under preparation: 1. To identify the origin of the virus: The virus identified was fully sequenced and compared with the sequences available in the GenBank. It appeared that while gene segment L2 was clustered with EHDV-7 isolated in Australia, most of the other segments were clustered with EHDV-6 isolates from South-Africa and Bahrain. This may suggest that the strain which affected Israel on 2006 may have been related to similar outbreaks which occurred in north-Africa at the same year and could also be a result of reassortment with an Australian strain (Wilson et al. article in preparation). Analysis of the serological results from Israel demonstrated that cows and calves were similarly positive as opposed to BTV for which seropositivity in cows was significantly higher than in calves. This finding also supports the hypothesis that the 2006 EHD outbreak in Israel was an incursive event and the virus was not present in Israel before this outbreak (Kedmi et al. Veterinary Journal, 2011) 2. To identify the vectors of this virus: In the US, Culicoides sonorensis was found as an efficient vector of EHDV as the virus was transmitted by midges fed on infected white tailed deer (WTD; Odocoileusvirginianus) to susceptible WTD (Ruder et al. Parasites and Vectors, 2012). We also examined the effect of temperature on replication of EHDV-7 in C. sonorensis and demonstrated that the time to detection of potentially competent midges decreased with increasing temperature (Ruder et al. in preparation). Although multiple attempts were made, we failed to evaluate wild-caught Culicoidesinsignisas a potential vector for EHDV-7; however, our finding that C. sonorensis is a competent vector is far more significant because this species is widespread in the U.S. As for Israeli Culicoides spp. the main species caught near farms affected during the outbreaks were C. imicolaand C. oxystoma. The vector competence studies performed in Israel were in a smaller scale than in the US due to lack of a laboratory colony of these species and due to lack of facilities to infect animals with vector borne diseases. However, we found both species to be susceptible for infection by EHDV. For C. oxystoma, 1/3 of the Culicoidesinfected were positive 11 days post feeding. 3. To identify the host and environmental factors influencing the level of exposure to EHDV, its spread and its associated morbidity: Analysis of the cattle morbidity in Israel showed that the disease resulted in an average loss of over 200 kg milk per cow in herds affected during September 2006 and 1.42% excess mortality in heavily infected herds (Kedmi et al. Journal of Dairy Science, 2010). Outbreak investigation showed that winds played a significant role in virus spread during the 2006 outbreak (Kedmi et al. Preventive Veterinary Medicine, 2010). Further studies showed that both sheep (Kedmi et al. Veterinary Microbiology, 2011) and wild ruminants did not play a significant role in virus spread in Israel (Kedmi et al. article in preparation). Clinical studies in WTD showed that this species is highly susceptibile to EHDV-7 infection and disease (Ruder et al. Journal of Wildlife Diseases, 2012). Experimental infection of Holstein cattle (cows and calves) yielded subclinical viremia (Ruder et al. in preparation). The findings of this study, which resulted in 6 articles, published in peer reviewed journals and 4 more articles which are in preparation, contributed to the dairy industry in Israel by defining the main factors associated with disease spread and assessment of disease impact. In the US, we demonstrated that sufficient conditions exist for potential virus establishment if EHDV-7 were introduced. The significant knowledge gained through this study will enable better decision making regarding prevention and control measures for EHDV and similar viruses, such as BTV.
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Sengupta-Gopalan, Champa, Shmuel Galili y Rachel Amir. Improving Methionine Content in Transgenic Forage Legumes. United States Department of Agriculture, febrero de 2001. http://dx.doi.org/10.32747/2001.7580671.bard.
Texto completoResumen
Leguminous forage crops are high in proteins but deficient in S- amino acids. It has been shown that both wool quality and milk production can be limited by the post-ruminal supply of sulfur-containing amino acids. Efforts to use conventional plant breeding and cell selection techniques to increase the S-amino acid content of alfalfa have met with little success. With the objective to increase the S-amino acid content of forage legumes, the goal of this project was to co- express the methionine rich zein genes from corn along with a gene for a key enzyme in methionine biosynthesis, aspartate kinase(AK). The zeins are seed storage proteins from corn and are groupec into four distinct classes based on their amino acid sequence homologies. The b-zein (15kd) and the 6zein (10kD and 18kD) have proportionately high levels of methionine (10%, 22% and 28%, respectively). Initial studies from our lab had shown that while the 15kD zein accumulated to high levels in vegetative tissues of transgenic tobacco the l0kD zein did not. However, co-expression of the 10kD zein with the 15kD zein genes in tobacco showed stabilization of the 10kD zein and the co-localization of the 10kD and 15kD zein proteins in unique ER derived protein bodies. AK is the key enzyme for producing carbon skeletons for all amino acids of the aspartate family including methionine. It is, however, regulated by end-product feedback inhibition. The specific objectives of this proposal were: i. to co-express the 15kD zein with the 10/18kD zein genes in alfalfa in order to enhance the level of accumulation of the 10/18kD zein; ii. to increase methionine pools by expressing a feedback insensitive AK gene in transformants co-expressing the 15kD and 10/18kD zein genes. The Israeli partners were successful in expressing the AK gene in alfalfa which resulted in an increase in free and bound threonine but not in methionine (Galili et al., 2000). Since our target was to increase methionine pools, we changed our second objective to replace the AK gene with the gene for cystathionine gamma synthase (CGS) in the co-expression studies. The first methionine specific reaction is catalyzed by CGS. An additional objective was to develop a transformation system for Berseem clover, and to introduce the appropriate gene constructs into it with the goal of improving their methionine content. Genes for the 15kD zein along with the genes for either the 10kD or 18kD zein have been introduced into the same alfalfa plant both by sexual crosses and by re-transformation. Analysis of these zein co-expressors have shown that both the IOkD and 18kD zein levels go up 5 to 10 fold when co-expressed with the 15kD zein (Bagga et al., MS in preparation). Incubation of the leaves of transgenic alfalfa co-expressing the 15kD and 10kD zein genes, in the rumen of cows have shown that the zein proteins are stable in the rumen. To increase the level of zein accumulation in transgenic alfalfa different promoters have been used to drive the zein genes in alfalfa and we have concluded that the CaMV 35S promoter is superior to the other strong leaf -specific promoters. By feeding callus tissue of alfalfa plants co-expressing the 15kD and 10kD zein genes with methionine and its precursors, we have shown that the zein levels could be significantly enhanced by increasing the methionine pools. We have now introduced the CGS gene (from Arabidopsis; kindly provided to us by Dr. Leustek), into the 15kD zein transformants and experiments are in progress to check if the expression of the CGS gene indeed increases the level of zein accumulation in alfalfa. We were not successful in developing a transformation protocol for Berseem clover.