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Literatura académica sobre el tema "RP-UPLC"

Autor: Grafiati

Publicado: 10 de marzo de 2023

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Artículos de revistas sobre el tema "RP-UPLC"

Bharani Pandilla, Chitra K, Nalini C N, Ashok P y Vadivelan R. "Method development and validation of Ifetroban by RP-UPLC". International Journal of Research in Pharmaceutical Sciences 11, n.º 3 (7 de julio de 2020): 3158–63. http://dx.doi.org/10.26452/ijrps.v11i3.2429.

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The purpose of this work is to develop and validate reverse phase Ultra performance liquid chromatography (UPLC) method for the rapid and precise determination of ifetroban sodium in its pure form and in formulations. A simple, specific, accurate, precise isocratic UPLC method for analysis of Ifetroban sodium was developed and validated using a Phenomenex C18 column (50 mm x 3.0 mm, 3µ) as the stationary phase, in conjunction using Triethyl amine buffer: methanol in the proportion of 25:75 with a flow rate of 1.0 mL/min, run time is 3 min and UV detector is used at 235 nm wavelength. The developed UPLC technique was found to be rapid as the retention time was 0.56 minutes for Ifetroban peak to elute. The developed UPLC technique was validated as per the ICH guidelines for specificity, linearity, accuracy, precision, robustness and found to be satisfactory. Linearity was established in the concentration range 100-300 µg/mL with correlation coefficient of 0.998 and the equation obtained is y = 0.635x + 0.639.The percentage recovery is 100.41. The method is rugged and is trouble free and transferable. The study showed that the developed UPLC technique can be used for the estimation of drug purity, stability, solubility and with no interference of pharmaceutical excipients from the active pharmaceutical ingredient. The precision, accuracy, robustness results obtained enables rapid quantification of ifetroban for quantitative analysis.

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Zhou, Jianwen, Caixia Han, Hui Cao, Shoumin Zhen, Zitong Yu, Xiaohui Li, Wujun Ma y Yueming Yan. "Fast identification of wheat 1BL.1RS translocation by reversed-phase ultra-performance liquid chromatography (RP-UPLC)". Crop and Pasture Science 64, n.º 9 (2013): 865. http://dx.doi.org/10.1071/cp13246.

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The 1BL.1RS chromosomal translocation in wheat is the result of replacement of the short arm of chromosome 1B of wheat by the short arm of chromosome 1R of rye, which had been widely used as a parental line in worldwide wheat breeding, resulting in a high percentage of wheat cultivars containing this translocation. A fast and reliable approach to identify this translocation is highly desirable in modern wheat breeding. This study compared reversed-phase ultra-performance liquid chromatography (RP-UPLC), acidic polyacrylamide gel electrophoresis (A-PAGE), liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), allelic-specific PCR, and reversed-phase high-performance liquid chromatography (RP-HPLC) approaches to identify the 1BL.1RS translocation in 76 bread wheat cultivars. Two gliadin bands in the Gli-B1 region of A-PAGE separation were confirmed by LC-MS/MS to be omega secalins from the 1BL.1RS translocation, and they can be used as reliable protein markers for identifying the translocation. A few specific minor peaks eluted at 12–13 min on the RP-UPLC patterns can readily differentiate the 1BL.1RS translocation. Of the 76 wheat cultivars tested, 40 were identified as carrying the 1BL.1RS translocation by RP-UPLC, which was consistent with the results of A-PAGE, HPLC, and PCR. Compared with other established methods, RP-UPLC showed a clear advantage in fast identification of the 1BL.1RS translocation with higher reliability and lower costs, and it is therefore ideal for large-scale screening of the 1BL.1RS translocation in wheat breeding.

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Takale, Nilesh, Neelakandan Kaliyaperumal, Gopalakrishnan Mannathusamy y Rajarajan Govindasamy. "Method Development and Validation for Quantitative Analysis of Anti-Histamine Promethazine Hydrochloride by RP-UPLC". Oriental Journal Of Chemistry 37, n.º 1 (28 de febrero de 2021): 33–39. http://dx.doi.org/10.13005/ojc/370103.

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The quantitative analysis method for the quantitative analysis of the anti-histaminic drug Promethazine Hydrochloride (PMZ•HCl) is stated by a straightforward, smooth, reliable and reverse step of the ultra-performing liquid chromatographic method (RP-UPLC). Following ICH quidelinesQ2(R1), the RP-UPLC method has been developed and checked. The uniform solution of 3.4% KH2PO4 solution in water, 7.0 pH with dilute KOH, ACN, andMeOH in ratio of 40:40:20, used as a mobile phase. The flow of 0.6 mL/minusing photo diode array detector / UV detector by with wavelength of 254 nm and runtime 3 min. This gives linearty from 80-120 % with correlation coefficient of 0.99988. Repeatability and intermediate precision shows relative standard deviation (percent RSD) of 0.52, 0.24 and a overall RSD of 0.43. Robustness studies show no indicative changes in SST requirements, like asymmetry factor, theoretical plate & percentage relative standard deviation. These criteria's values are well within their acceptability limit. The degradation of promethazine under different stress conditions has been studied and shows that all known impurities and degradants are well separated from promethazine peak.This RP-UPLC is descriptive and accurate.

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Vishnuvardhan, Chiguru, R. Srinivas y N. Satheeshkumar. "Development and validation of a UPLC method for screening potentially counterfeit anti-hypertensive drugs using design of experiment". Anal. Methods 6, n.º 13 (2014): 4610–16. http://dx.doi.org/10.1039/c4ay00384e.

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Lakshmi, D. Sri, Jane T. Jacob, D. Srinivasa Sastry y D. Satyanarayana. "Simultaneous Estimation of Metformin Glimeperide and Voglibose by RP-UPLC". Asian Journal of Pharmaceutical Analysis 7, n.º 1 (2017): 23. http://dx.doi.org/10.5958/2231-5675.2017.00005.9.

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Kumar, Kakumani Kishore, Chimalakonda Kameswara Rao, Maddala Vijaya Lakshmi y Khagga Mukkanti. "A Validated Stability Indicating RP-UPLC Method for Atrovastain Calcium". American Journal of Analytical Chemistry 03, n.º 05 (2012): 392–99. http://dx.doi.org/10.4236/ajac.2012.35052.

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Dubey, Somshankar y Mahesh Duggirala. "SIMULTANEOUS ESTIMATION OF LAMIVUDINE, ABACAVIR AND DOLUTEGRAVIR BY UPLC METHOD". International Journal of Applied Pharmaceutics 10, n.º 1 (6 de enero de 2018): 46. http://dx.doi.org/10.22159/ijap.2018v10i1.21156.

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Objective: To develop a simple, rapid, sensitive and effective reverse phase ultra-performance liquid chromatographic method (RP-UPLC) for simultaneous quantification of lamivudine, abacavir and dolutegravir in pure and tablet dosage forms.Methods: Chromatographic separation was performed by using Waters-ACQUITY UPLC system equipped with auto sampler, photodiode array (PDA) detector, zodiac sil RP C18 (4.6 mm × 250 mm, 3.0 µm) column, phosphate buffer (pH 3.0) and methanol in the ratio of 30:70 %v/v have been delivered at a flow rate of 0.25 ml/min and the detection was carried out using a Ultraviolet (UV) detector at a wavelength of 260 nm at ambient column temperature. The mobile phase was used as diluent.Results: The retention time (Rt) for lamivudine, abacavir and dolutegravir were 1.763, 2.247 and 3.175 min respectively. A good linear response was obtained in the range of 15-75 µg/ml, 30-150 µg/ml and 2.5-12.5 µg/ml, respectively. The Limit of Detection (LOD) values were found to be 0.021, 0.330 and 0.038 µg/ml, respectively and the Limit of Quantitation (LOQ) values were 0.056, 1.320 and 0.095 µg/ml, respectively.Conclusion: It was concluded that the developed RP-UPLC method was effective, suitable and conducive for analyzing lamivudine, abacavir and dolutegravir in pharmaceutical formulations. The method was quantitatively evaluated in terms of precision, linearity, accuracy (recovery), selectivity and robustness in accordance with standard guidelines.

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Rao, Sanapala Srinivasa y A. Vijayalakshmi. "Analytical method development and validation of glipizide by RP-UPLC method". RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY 14, n.º 3 (2021): 1370–74. http://dx.doi.org/10.5958/0974-360x.2021.00244.4.

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Raju Sri Datla, V. V. S. S. N. y Manikandan Ayyar. "Development and validation of Stability-indicating RP-UPLC Method for the Simultaneous Determination of Ivacaftor, Tezacaftor and Elexacaftor in bulk and their Formulation". Research Journal of Chemistry and Environment 25, n.º 12 (25 de noviembre de 2021): 107–15. http://dx.doi.org/10.25303/2512rjce107115.

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A simple reproducible stability indicating RP-UPLC method was developed for the simultaneous determination of Ivacaftor, Tezacaftor and Elexacaftor in their combined dosage forms using HSS C18, 1.8μm, 100mm x2.1 mm i.d. column. A mobile phase of phosphate buffer (10mM) pH-4.8 and acetonitrile in the ratio of 70: 30v/v mixture was used for separation and quantification of ivacaftor, tezacaftor and elexacaftor. The present drug analytes were run at a flow-rate of 0.3ml/ min at 30°C temperature. The injection volume was 2μL and with ultraviolet detection at 270nm. Under these conditions, elexacaftor, ivacaftor and tezacaftor were eluted at 0.72min, 1.4min and 1.9min respectively with a total run time shorter than 5min. The developed method was validated according to International Conference on Harmonization (ICH) guidelines. The developed RP-UPLC method was applied successfully for quality control assay of Ivacaftor, Tezacaftor and Elexacaftor in their combination drug product.

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Lee, Su-Bin, Yu-Jeong Yang, Sun-Hyung Lim, Yong Q. Gu y Jong-Yeol Lee. "A Rapid, Reliable RP-UPLC Method for Large-Scale Analysis of Wheat HMW-GS Alleles". Molecules 26, n.º 20 (13 de octubre de 2021): 6174. http://dx.doi.org/10.3390/molecules26206174.

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High-molecular-weight glutenin subunits (HMW-GS) account for only 10% of total wheat storage proteins, but play an important role in the processing quality of wheat flour. Therefore, identifying HMW-GS alleles associated with good end-use quality provides important information for wheat breeders. To rapidly, accurately and reproducibly identify HMW-GS, we established an optimized reversed-phase ultra-performance liquid chromatography (RP-UPLC) method. Separation parameters were optimized using an ACQUITY UPLC Protein BEH C4 column and stepwise ACN gradient, and the separation patterns and retention times (RTs) of 22 subunits were comparatively analyzed in 16 standard wheat cultivars. All HMW-GS proteins were well separated within about 5.5 min, and all analyses were complete within 12 min. We distinguished the 16 subunits based on RT, although three subunits in 1Bx (1Bx7/1Bx7OE and 1Bx17) and three subunits in 1By (1By8*, 1By9 and 1By15) had overlapping RTs; these were differentiated by SDS-PAGE. To distinguish 1Bx7 and 1Bx7OE, which differ in protein abundance, RP-UPLC was combined with PCR analysis of DNA junction markers. The optimized method was successfully applied to determine HMW-GS alleles in a large collection of bread wheat germplasm (1787 lines). This protocol is an appropriate option for selecting lines harboring favorable HMW-GS alleles in wheat breeding.

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Tesis sobre el tema "RP-UPLC"

Saviano, Alessandro Morais. "Métodos espectrofotométrico, RP-UPLC e microbiológico para determinação de linezolida em formas farmacêuticas". Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/9/9139/tde-26102015-094146/.

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A linezolida é um antibiótico oxazolidinona efetivo contra bactérias patogênicas gram-positivas, incluindo Staphylococcus aureus resistente a meticilina (MRSA), Staphylococcus aureus resistente a glicopeptídeos (GISA) e enterococci resistente a vancomicina (VRE). As oxazolidinonas inibem a translação bacteriana na fase de iniciação da síntese de proteínas, enquanto que a síntese de RNA e DNA não são afetadas. Diversos métodos por cromatografia líquida de alta-eficiência (HPLC) têm sido relatados para a determinação da concentração de linezolida em plasma humano, soro e urina. Entretanto, um número pequeno de métodos estão disponíveis para a determinação de linezolida em formulações farmacêuticas. O objetivo deste trabalho foi desenvolver e validar métodos espectrofotométrico, de cromatografia líquida de ultraeficiência em fase reversa (RP-UPLC), doseamento microbiológico em placas e doseamento microbiológico rápido em microplacas com leitura cinética para a determinação de linezolida em soluções injetáveis e comprimidos. Adicionalmente, foram identificadas e quantificadas as principais fontes de incerteza relacionadas aos métodos. O desenvolvimento dos métodos utilizou uma abordagem racional, empregando-se planejamento de experimentos (análise fatorial e metodologia de superfície resposta) para otimização das condições analíticas. As fontes de incertezas foram identificadas empregando-se diagrama de Ishikawa e quantificadas conforme procedimento da Eurachem/Citac. Os métodos desenvolvidos apresentaram especificidade/seletividade, linearidade, precisão, exatidão e robustez adequadas, conforme os critérios estabelecidos pelo ICH e ANVISA. A análise estatística demonstrou equivalência entre os resultados providos por cada um dos métodos, sendo intercambiáveis entre si. Portanto, os métodos podem ser empregados no controle de qualidade de rotina na indústria farmacêutica.
Linezolid is an oxazolidinone antibiotic effective against gram-positive pathogenic bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), glycopeptide-intermediate Staphylococcus aureus (GISA) and vancomycin-resistant enterococci (VRE). Oxazolidinones inhibit bacterial translation at the initiation phase of protein synthesis, while RNA and DNA synthesis are not affected. Several high performance liquid chromatographic (HPLC) methods have been reported to determine the concentration of linezolid in human plasma, serum and urine. However, a few number of methods are available to determine the concentration of linezolid in pharmaceutical dosage forms. The aim of the present work was to develop and validate a spectrophotometric method, a reverse-phase ultra-high performance liquid chromatographic method, microbiological assay and microbiological rapid assay in microplates using kinetic reading for the determination of linezolid in injections and tablets dosage forms. In addition, the most important sources of uncertainty were identified and quantified for each method. A rational approach was used in the development of these methods, using design of experiments (factorial design and response surface methodology) for optimization of analytical conditions. The sources of uncertainties were identified using Ishikawa diagram and quantified as described in Eurachem/Citac procedure. The developed methods were specific/selective, linear, precise, accurate and robust, as required by ICH and ANVISA guidelines. Statistical analysis showed equivalence among the results provided by each method, being interchangeable. Thus, the methods may be employed in routine quality control analysis by pharmaceutical industry.

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Ida, Björs. "Development of separation method for analysis of oligonucleotides using LC-UV/MS". Thesis, Uppsala universitet, Analytisk farmaceutisk kemi, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-403381.

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Introduction Oligonucleotides are short nucleic acid chains, usually 19-27mer long. They bind to their corresponding chain, making a specific inhibition possible. In pharmaceuticals, this can be used to inhibit the expression of a gene or protein of interest. Oligonucleotides are usually analyzed based on separation using both hydrophobic and ion-exchange properties. In this project, the possibility to use a mixed-mode column to separate these oligonucleotides and their impurities were explored. Method Liquid chromatography is used as the separation method and the method of detection is both mass spectrometry and UV. Three different columns are evaluated; C18, DNAPac RP, and mixed-mode RP/WAX. Results and discussion Different compositions of mobile phases and gradients are evaluated based on a literature study. Triethylamine, triethylammonium acetate, ammonium formate, hexafluoroisopropanol is used along with both methanol and acetonitrile. Phosphate buffer is evaluated on LC-UV. The results from the C18 column displays a good separation of the oligonucleotides, whilst the DNAPac RP is not as sufficient using the same mobile phases. The mixed-mode column provides good separation and selectivity using phosphate buffer and UV detection. Conclusion Mixed-mode column has the potential to be used for separation of oligonucleotides and one future focus would be to make the mobile phase compatible with mass spectrometry. Phosphate buffer and UV detection seems to be the go-to mobile phase using mixed-mode column even though MS is a more powerful tool for the characterization and identification of oligonucleotides. This provides a hint about the challenge in making the mobile phase MS compatible.

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Luca, Nompari. "Designing Quality: Quality by Design in the analytical pharmaceutical development". Doctoral thesis, 2018. http://hdl.handle.net/2158/1116278.

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The present thesis study describes an integrated approach for the implementation of Quality by Design principles to screening and development of analytical methods applied to vaccines physical-chemical testing, according to Analytical QbD (AQbD) published in literature. The research study is focused on application of the risk assessment and the experimental design tools to be used to build knowledge about the analytical methods performance and to mitigate the risks of failure. Risk management tools and mathematical/statistical models have been applied to study and define well characterized analytical methods (based on understanding of method parameters-performance relationships), in order to establish an analytical procedure able to ensure the desired performance and to identify the method design space. The AQbD structured approach clearly mitigates the risk of failure and provides advantages in a life-cycle management prospective.

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Actas de conferencias sobre el tema "RP-UPLC"

Reinoso, Zain Sanchez, Jacinthe Thibodeau, Laila Ben Said, Ismail Fliss, Laurent Bazinet y Sergey Mikhaylin. "Bioactive Peptide Production from Slaughterhouse Blood Proteins: Impact of Pulsed Electric Fields and Ph on Enzyme Inactivation, Antimicrobial and Antioxidant Activities of Peptic Hydrolysates from Bovine and Porcine Hemoglobins". En 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/fsht2150.

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Slaughterhouse blood is a valuable by-product since multiple bioactive compounds can be derived out of it. Its solid fraction consists mainly of hemoglobin, which is a good source of antimicrobial and antioxidant peptides that can be released by peptic hydrolysis. Nevertheless, this method has limitations such as low yield, expensive cost of enzyme process, and non-eco-friendly production (high energy consumption and chemical reagents requested). Amount the alternative green technologies for protein valorization, pulsed electric field (PEF) stands out since it allows modifying the physicochemical properties of proteins, promoting the enzymatic hydrolysis, enzyme inactivation, and bioactivity enhancement. Thus, this study aimed to evaluate the effect of PEF on the pepsin inactivation and biological activities (antimicrobial and antioxidant) in hemoglobin hydrolysates. Bovine and porcine hemoglobins were hydrolyzed with pepsin for 3 h (37°C, pH 3.0) and treated with PEF (73 pulses, 23.8kV/cm, 90Hz) to inactivate the enzyme. The hydrolysis degree was evaluated, which did not show significant changes after PEF-inactivation of pepsin, whereas the peptide population analysis by RP-UPLC-MS/MS showed some changes in PEF-treated hydrolysates over time, which suggested a residual pepsin activity. Additionally, the impact of pH (3, 7, and 10) on bioactivity was studied. PEF-treatments did not show a significant impact on antimicrobial (antibacterial, antifungal, and anti-yeast activities) and antioxidant activities (DPPH and ORAC). However, higher pH fostered stronger anti-yeast activity (R. mucilaginosa) and DPPH‐scavenging capacity, whereas pH 7 fostered the antifungal activity (M. racemosus). Even though some changes were observed in the peptide population, no negative effects of PEF were found for biological activities. Thus, the utilization of hemoglobin from the meat industry combined with PEF-treatment fits the circular economy concept since derived peptides can be recycled to protect meat and other products against microbial growth and oxidation.

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