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1

Capretti, Paolo, Cecilia Comparini, Matteo Garbelotto y Nicola La Porta, eds. XIII Conference "Root and Butt Rot of Forest Trees" IUFRO Working Party 7.02.01. Florence: Firenze University Press, 2013. http://dx.doi.org/10.36253/978-88-6655-353-3.

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The present Proceedings comprise the contributions that were presented at the 13th International Conference of the IUFRO W. Party 7.02.01 “Root and Butt Rot of Forest Trees” that was held in Italy from the 4th to the 10th of September 2011. The Conference started in Firenze than moved to FEM Research Centre, S. Michele all’Adige, Trento and continued in San Martino di Castrozza, Dolomite region. Root and Butt Rot of Forest Trees have a high biological and economic impact in forestry. The Proceedings were organized under seven headings: Genomics and Plant-Pathogen Interactions; Systematics, Taxonomy and Phylogeography; Ecology; Population Genetics; Etiology and Epidemiology; Disease Management and Control; New Reports, Diagnostics and Research on the Application of new Diagnostic Methods.
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2

Thies, Walter Gene. Laminated root rot in Western North America. Portland, Or: U.S. Dept. of Agriculture, Forest Service, Pacific Northwest Research Station, 1995.

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3

Peterson, Michael James. Sanitation of styroblocks to control algae and seedling root rot fungi. Victoria, B.C: Forestry Canada, 1990.

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4

L, Campbell C. y Benson D. M. 1945-, eds. Epidemiology and management of root diseases. Berlin: Springer-Verlag, 1994.

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5

Geiger, Jean-Paul. Maladies racinaires de l'hévéa: Biochimie et physiologie des relations hôte-parasite. Paris: Editions de l'ORSTOM, 1987.

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6

Jacobs, Karin. Leptographium species: Tree pathogens, insect associates, and agents of blue-stain. St. Paul, Minn: APS Press, American Phytopathological Society, 2001.

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7

James, Robert L. Effects of pre-sowing soil treatments on root colonization of 1-0 ponderosa and lodgepole pine seedlings by potentially-pathogenic fungi, USDA Forest Service Lucky Peak Nursery, Boise, Idaho. Missoula, MT: U.S. Dept. of Agriculture, Forest Service, Northern Region, 2001.

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8

James, Robert L. Effects of pre-sowing soil treatments on root colonization of 1-0 ponderosa and lodgepole pine seedlings by potentially-pathogenic fungi, USDA Forest Service Lucky Peak Nursery, Boise, Idaho. Missoula, MT: U.S. Dept. of Agriculture, Forest Service, Northern Region, 2001.

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9

Mallett, K. I. The Trap-log method of detecting armillaria root rot pathogens in forest soils (Forest management note). Forestry Canada, Northwest Region, Northern Forest Centre, 1991.

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10

Krupa, S. V. Ecology of Root Pathogens. Elsevier, 2012.

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11

Laminated root rot in Western North America. [Portland, Or.]: U.S. Dept. of Agriculture, Forest Service, Pacific Northwest Research Station, 1995.

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12

Gleń-Karolczyk, Katarzyna. Zabiegi ochronne kształtujące plonowanie zdrowotność oraz różnorodność mikroorganizmów związanych z czernieniem pierścieniowym korzeni chrzanu (Atmoracia rusticana Gaertn.). Publishing House of the University of Agriculture in Krakow, 2019. http://dx.doi.org/10.15576/978-83-66602-39-7.

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Horseradish roots, due to the content of many valuable nutrients and substances with healing and pro-health properties, are used more and more in medicine, food industry and cosmetics. In Poland, the cultivation of horseradish is considered minor crops. In addition, its limited size causes horseradish producers to encounter a number of unresolved agrotechnical problems. Infectious diseases developing on the leaves and roots during the long growing season reduce the size and quality of root crops. The small range of protection products intended for use in the cultivation of horseradish generates further serious environmental problems (immunization of pathogens, low effectiveness, deterioration of the quality of raw materials intended for industry, destruction of beneficial organisms and biodiversity). In order to meet the problems encountered by horseradish producers and taking into account the lack of data on: yielding, occurrence of infectious diseases and the possibility of combating them with methods alternative to chemical ones in the years 2012–2015, rigorous experiments have been carried out. The paper compares the impact of chemical protection and its reduced variants with biological protection on: total yield of horseradish roots and its structure. The intensification of infectious diseases on horseradish leaves and roots was analyzed extensively. Correlations were examined between individual disease entities and total yield and separated root fractions. A very important and innovative part of the work was to learn about the microbial communities involved in the epidemiology of Verticillium wilt of horseradish roots. The effect was examined of treatment of horseradish cuttings with a biological preparation (Pythium oligandrum), a chemical preparation (thiophanate-methyl), and the Kelpak SL biostimulator (auxins and cytokinins from the Ecklonia maxima algae) on the quantitative and qualitative changes occurring in the communities of these microorganisms. The affiliation of species to groups of frequencies was arranged hierarchically, and the biodiversity of these communities was expressed by the following indicators: Simpson index, Shannon–Wiener index, Shannon evenness index and species richness index. Correlations were assessed between the number of communities, indicators of their biodiversity and intensification of Verticillium wilt of horseradish roots. It was shown that the total yield of horseradish roots was on average 126 dt · ha–1. Within its structure, the main root was 56%, whereas the fraction of lateral roots (cuttings) with a length of more than 20 cm accounted for 26%, and those shorter than 20 cm for 12%, with unprofitable yield (waste) of 6%. In the years with higher humidity, the total root yield was higher than in the dry seasons by around 51 dt · ha–1 on average. On the other hand, the applied protection treatments significantly increased the total yield of horseradish roots from 4,6 to 45,3 dt · ha–1 and the share of fractions of more than 30 cm therein. Higher yielding effects were obtained in variants with a reduced amount of foliar application of fungicides at the expense of introducing biopreparations and biostimulators (R1, R2, R3) and in chemical protection (Ch) than in biological protection (B1, B2) and with the limitation of treatments only to the treatment of cuttings. The largest increments can be expected after treating the seedlings with Topsin M 500 SC and spraying the leaves: 1 × Amistar Opti 480 SC, 1 × Polyversum WP, 1 × Timorex Gold 24 EC and three times with biostimulators (2 × Kelpak SL + 1 × Tytanit). In the perspective of the increasing water deficit, among the biological protection methods, the (B2) variant with the treatment of seedlings with auxins and cytokinins contained in the E. maxima algae extract is more recommended than (B1) involving the use of P. oligandrum spores. White rust was the biggest threat on horseradish plantations, whereas the following occurred to a lesser extent: Phoma leaf spot, Cylindrosporium disease, Alternaria black spot and Verticillium wilt. In turn, on the surface of the roots it was dry root rot and inside – Verticillium wilt of horseradish roots. The best health of the leaves and roots was ensured by full chemical protection (cuttings treatment + 6 foliar applications). A similar effect of protection against Albugo candida and Pyrenopeziza brassicae was achieved in the case of reduced chemical protection to one foliar treatment with synthetic fungicide, two treatments with biological preparations (Polyversum WP and Timorex Gold 24 EC) and three treatments with biostimulators (2 × Kelpak SL, 1 × Tytanit). On the other hand, the level of limitation of root diseases comparable with chemical protection was ensured by its reduced variants R3 and R2, and in the case of dry root rot, also both variants of biological protection. In the dry years, over 60% of the roots showed symptoms of Verticillium wilt, and its main culprits are Verticillium dahliae (37.4%), Globisporangium irregulare (7.2%), Ilyonectria destructans (7.0%), Fusarium acuminatum (6.7%), Rhizoctonia solani (6.0%), Epicoccum nigrum (5.4%), Alternaria brassicae (5.17%). The Kelpak SL biostimulator and the Polyversum WP biological preparation contributed to the increased biodiversity of microbial communities associated with Verticillium wilt of horseradish roots. In turn, along with its increase, the intensification of the disease symptoms decreased. There was a significant correlation between the richness of species in the communities of microbial isolates and the intensification of Verticillium wilt of horseradish roots. Each additional species of microorganism contributed to the reduction of disease intensification by 1,19%.
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13

Mazzola, Mark. The effect of fungal root pathogens and phenazine antibiotic biosynthesis on the survival of fluorescent pseudomonads in soil habitats. 1990.

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14

Garrett, S. D. Pathogenic Root-Infecting Fungi. University of Cambridge ESOL Examinations, 2011.

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15

Marsden, M. A. Sensitivity of the western root disease model: inventory of root disease. 1992.

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16

Brammall, Ronald Anthony. Host-pathogen interactions in Fusarium crown and root rot disease in tomato. 1987.

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17

Bae, Hanhong. RFLP analysis of genetic variation in the laminated-root-rot fungal pathogen of conifers, Phellinus weirii. 1992.

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18

Leptographium Species: Tree Pathogens, Insect Associates, and Agents of Blue-Stain. Amer Phytopathological Society, 2001.

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19

Ferguson, Brennan Angus. Fungal root pathogen interactions in a mixed conifer forest in the Blue Mountains of northeastern Oregon. 1994.

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20

Stirling, Graham, Helen Hayden, Tony Pattison y Marcelle Stirling. Soil Health, Soil Biology, Soilborne Diseases and Sustainable Agriculture. CSIRO Publishing, 2016. http://dx.doi.org/10.1071/9781486303052.

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Our capacity to maintain world food production depends heavily on the thin layer of soil covering the Earth's surface. The health of this soil determines whether crops can grow successfully, whether a farm business is profitable and whether an enterprise is sustainable in the long term. Farmers are generally aware of the physical and chemical factors that limit the productivity of their soils but often do not recognise that soil microbes and the soil fauna play a major role in achieving healthy soils and healthy crops. Soil Health, Soil Biology, Soilborne Diseases and Sustainable Agriculture provides readily understandable information about the bacteria, fungi, nematodes and other soil organisms that not only harm food crops but also help them take up water and nutrients and protect them from root diseases. Complete with illustrations and practical case studies, it provides growers and their consultants with holistic solutions for building an active and diverse soil biological community capable of improving soil structure, enhancing plant nutrient uptake and suppressing root pests and pathogens. The book is written by scientists with many years' experience developing sustainable crop production practices in the grains, vegetable, sugarcane, grazing and horticultural industries. This book will be useful for: growers, consultants, agronomists and soil chemists, extension personnel working in the grains, livestock, sugarcane and horticultural industries, professionals running courses in soil health/biological farming, and students taking university courses in soil science, ecology, microbiology, plant pathology and other biological sciences.
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21

Kuchta, David. Effects of rhizobium phaseoli (6-3) inoculation on the root-rot pathogen fusarium oxysporum in broth culture and in the rhizosphere and spermosphere of greenhouse and field cultivated phaseolus vulgaris: A thesis in biology. 1985.

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