Literatura académica sobre el tema "Real-time PCR"
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Artículos de revistas sobre el tema "Real-time PCR"
RAZA, ABIDA y NAUREEN A KHATTAK. "REAL TIME PCR;". Professional Medical Journal 19, n.º 06 (3 de noviembre de 2012): 751–59. http://dx.doi.org/10.29309/tpmj/2012.19.06.2455.
Texto completoLederman, Lynne. "Real-Time PCR". BioTechniques 44, n.º 2 (febrero de 2008): 179–83. http://dx.doi.org/10.2144/000112741.
Texto completoDavidson, Eugene A. "REAL-TIME PCR". Shock 27, n.º 6 (junio de 2007): 708. http://dx.doi.org/10.1097/01.shk.0000270193.65250.8e.
Texto completoKusser, Wolfgang, Sandrine Javorschi y Martin A. Gleeson. "Real-Time PCR". Cold Spring Harbor Protocols 2006, n.º 1 (1 de enero de 2006): pdb.prot4112. http://dx.doi.org/10.1101/pdb.prot4112.
Texto completoFraga, Dean, Tea Meulia y Steven Fenster. "Real-Time PCR". Current Protocols Essential Laboratory Techniques 00, n.º 1 (enero de 2008): 10.3.1–10.3.34. http://dx.doi.org/10.1002/9780470089941.et1003s00.
Texto completoBusch, Ulrich. "Real-Time PCR". Journal für Verbraucherschutz und Lebensmittelsicherheit 2, n.º 2 (mayo de 2007): 111–12. http://dx.doi.org/10.1007/s00003-007-0178-7.
Texto completoKang, Won, Sang-Bum Park, Youn-Hyoung Nam, Young-Chang An, Sang-Hyun Lee, Won-Cheoul Jang, Su-Min Park, Jong-Wan Kim y Song-Chun Chong. "Detection of Hepatitis B Virus Using Micro-PCR and Real-Time PCR Methods". Journal of the Korean Chemical Society 51, n.º 1 (20 de febrero de 2007): 36–42. http://dx.doi.org/10.5012/jkcs.2007.51.1.036.
Texto completoHeid, C. A., J. Stevens, K. J. Livak y P. M. Williams. "Real time quantitative PCR." Genome Research 6, n.º 10 (1 de octubre de 1996): 986–94. http://dx.doi.org/10.1101/gr.6.10.986.
Texto completoSchmittgen, Thomas D. "Real-Time Quantitative PCR". Methods 25, n.º 4 (diciembre de 2001): 383–85. http://dx.doi.org/10.1006/meth.2001.1260.
Texto completoGospodinović, Hristina, Ljiljana Pavlović, Marija Obradović, Sanja Dimitrijević, Sofija Jovanović y Edita Grego. "Detection of high-risk HPV genotypes using Real-time PCR". Glasnik javnog zdravlja 96, n.º 4 (2022): 416–26. http://dx.doi.org/10.5937/serbjph2204416g.
Texto completoTesis sobre el tema "Real-time PCR"
Crane, Bryan Lee 1976. "Real time PCR measurement by fluorescence anisotropy". Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/30347.
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Includes bibliographical references (p. 181-190).
Real-time polymerase chain reaction (PCR) is the gold-standard for quantitation in both mutation and gene expression analyses. Already this technique has found valuable clinical application in disease diagnosis and progression evaluation. As the number of known gene-disease correlations continues to rise, there will be increased demand for higher throughput and decreased cost for these analyses. Present real-time PCR measurement is based upon the fluorescent intensity of either intercalating dyes or oligonucleotide probes. Intercalating dye methods suffer from a lack of binding specificity, while probe methods are expensive and require increased assay optimization. In this thesis, a new method is presented for monitoring real-time PCR that utilizes the fluorescent anisotropy (FA) of labeled primers. FA, when measured at constant temperature, is indicative of the molecular mass to which the fluorophore is attached. Specificity is improved with the FA method over the use of intercalating dyes since the selective binding of primers is required for signal change. Assay complexity and cost are reduced compared to fluorogenic probe methods since the probes are eliminated. The design of a prototype instrument, which successfully implements this new method, is presented. Instrument and assay performance are compared to intercalating dye assays run in commercially available instrumentation. Theoretical limits on performance are also presented and compared to experimental results. Excellent repeatability and linearity are observed with respect to these benchmarks. This new method, having both high specificity and low optimization complexity, is expected to be particularly applicable to the demanding robustness requirements of nano-scale PCR.
by Bryan Lee Crane.
Ph.D.
Rozales, Franciéli Pedrotti. "Real time-pcr e nested-pcr no diagnóstico da tuberculose pulmonar". reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/72990.
Texto completoTuberculosis (TB) remains as an important public health problem worldwide. Therefore, the rapid detection of M. tuberculosis is of primary importance to effectively reduce transmission among patients. The aims of this study were to evaluate two molecular tests to detect M. tuberculosis complex (MTBC) directly from clinical samples. The study included 124 respiratory samples which were evaluated by two in house molecular assays for MTBC detection: Nested PCR (NPCR) and Real Time PCR (RT-PCR). The respiratory samples were also evaluated by the direct test (AFB assay). The results were compared with the results of culture and also compared with the culture results plus clinical data of patients. We used a commercial DNA sample with known quantification to establish the Limit of Detection (LOD). The LOD was 1 copy/μL for RT-PCR and 25 copies/μL for NPCR. The AFB assay presented low sensitivity – SE - (40%) and a high specificity - SP – (94%). Both molecular assays, RT-PCR and NPCR presented high SE and SP (RT-PCR 98% and 91%, NPCR 86% and 93%, respectively) compared to culture. When the results of the molecular tests were compared to the culture plus clinical data the SE and SP were 90,20% and 97,26% for RT-PCR and 80,39% and 98,63% for the NPCR, respectively. It was possible to observe a slight decrease of SE of the molecular methods in comparison to culture plus clinical data in relation to culture; however, the SP was increased, since many cases of TB could not be confirmed by culture. Furthermore we evaluated the cost of molecular assays: the NPCR cost was $17.77/test while the RT-PCR cost was $15.76/test. The RT-PCR test was faster (2 hours) than the NPCR (4 hours) to be performed. Our study confirms that PCRs may be useful for rapid diagnosis of respiratory TB, with high SP rates. It may also be very important to exclude such diagnosis, considering the high NPV found in our study. In summary, PCRs targeting IS6110 of MTB improve the accuracy of the diagnosis of pulmonary TB, with many potential positive effects for clinical management and control of the disease.
Pires, Elisabete Sofia Videira. "Real-Time PCR, High Resolution Melting - aplicações forenses". Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/10747.
Texto completoApontada como a maior revolução científica na área forense desde a descoberta das impressões digitais, a identificação humana por meio da análise do DNA tornou-se uma poderosa ferramenta de investigação, auxiliando na elucidação de casos forenses, baseando-se cientificamente na existência de polimorfismos genéticos ao longo do genoma em indivíduos diferentes, que faz com que cada pessoa possua um código genético único. Com a introdução da real-time PCR nas investigações forenses, tornou-se possível uma análise sensível e específica de regiões polimórficas tanto no genoma nuclear como no mitocondrial, a partir de quantidades ínfimas de DNA obtidas de amostras altamente degradadas ou com baixo número de cópias. A quantificação do DNA é um procedimento importante na análise forense e deve ser efetuado, previamente, a qualquer análise de DNA. A união entre a bioinformática e a genética forense propiciou a criação de métodos de análise específicos, como a HRM, muito útil na genotipagem de SNPs, de extrema importância na investigação forense. Foi elaborada uma revisão bibliográfica com o objetivo de conhecer as aplicações forenses da real-time PCR e os respetivos métodos, tendo se confirmado então a aplicabilidade deste método na área forense.
Listed as the greatest revolution in forensic science since the discovery of fingerprints, identification by analyzing human DNA has become a powerful research tool, helping to elucidate forensic cases, scientifically based on the existence of genetic polymorphisms throughout the genome at different individuals, which causes that each person has a unique genetic code. With the introduction of real-time PCR in forensic investigations, it became possible a sensitive and specific analysis of polymorphic regions both in the mitochondrial and nuclear genome, from minute quantities of DNA obtained from samples highly degraded or low copy number. The quantification of DNA is an important procedure in forensic analysis and must be made in advance to any DNA analysis. The union between forensic genetics and bioinformatics led to the creation of specific analysis methods, such as HRM, very useful in scanning and genotyping of SNPs, of utmost importance in forensic investigation. A literature review has been prepared in order to meet the forensic applications of real-time PCR and related methods, and so been confirmed the applicability of this method in the forensic field.
Dunkley, Kingsley Delroy. "Modulation of cell yields and genetic responses of Salmonella fermentation and colonization in the gastrointestinal ecology of avian species". [College Station, Tex. : Texas A&M University, 2006. http://hdl.handle.net/1969.1/ETD-TAMU-1187.
Texto completoAndalo, Alice. "Analisi quantitativa dell'espressione genica mediante real-time rt-pcr". Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/8450/.
Texto completoDörries, Hans-Henno. "Entwicklung von Real-Time-PCR-Nachweissystemen für getränkerelevante Hefen". [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=979663938.
Texto completoHartmann, Britta. "Entwicklung einer Real-time-PCR-Nachweismethode für Yersinia enterocolitica". [S.l.] : [s.n.], 2007. http://edoc.ub.uni-muenchen.de/archive/00006660.
Texto completoHartmann, Britta. "Entwicklung einer Real-Time PCR-Nachweismethode für Yersinia enterocolitica". Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-66603.
Texto completoMalatji, Dikeledi Petunia. "Detection of Babesia rossi genotypes using real-time PCR". Diss., University of Pretoria, 2011. http://hdl.handle.net/2263/31138.
Texto completoDissertation (MSc)--University of Pretoria, 2011.
Veterinary Tropical Diseases
MSc
Unrestricted
Zhang, Yan. "Frequent RASSF1A gene promoter hypermethylation in breast cancer". [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-63611.
Texto completoLibros sobre el tema "Real-time PCR"
Tevfik, Dorak M., ed. Real-time PCR. New York: Taylor & Francis, 2006.
Buscar texto completoTevfik, Dorak M., ed. Real-time PCR. New York: Taylor & Francis, 2006.
Buscar texto completoBiassoni, Roberto y Alessandro Raso, eds. Quantitative Real-Time PCR. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0733-5.
Texto completoBiassoni, Roberto y Alessandro Raso, eds. Quantitative Real-Time PCR. New York, NY: Springer New York, 2020. http://dx.doi.org/10.1007/978-1-4939-9833-3.
Texto completoMeuer, Stefan, Carl Wittwer y Kan-Ichi Nakagawara, eds. Rapid Cycle Real-Time PCR. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0.
Texto completoQuantitative real-time PCR in applied microbiology. Norfolk, UK: Caister Academic Press, 2012.
Buscar texto completoJulie, Logan, Edwards Kirstin y Saunders Nick, eds. Real-time PCR: Current technology and applications. Norfolk, UK: Caister Academic Press, 2009.
Buscar texto completoQuantitative real-time PCR: Methods and protocols. New York: Humana Press, 2014.
Buscar texto completoDietmaier, Wolfgang, Carl Wittwer y Natarajan Sivasubramanian, eds. Rapid Cycle Real-Time PCR — Methods and Applications. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-59397-0.
Texto completoWittwer, Carl, Meinhard Hahn y Karen Kaul, eds. Rapid Cycle Real-Time PCR — Methods and Applications. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18840-4.
Texto completoCapítulos de libros sobre el tema "Real-time PCR"
Müller, Hans-Joachim y Daniel Ruben Prange. "Real-Time-PCR". En PCR - Polymerase-Kettenreaktion, 65–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-48236-0_14.
Texto completoMathew, Alan G. "Real-Time PCR". En Handbook of Food Safety Engineering, 217–57. Oxford, UK: Wiley-Blackwell, 2012. http://dx.doi.org/10.1002/9781444355321.ch10.
Texto completoSaunders, Nicholas A. "Real-Time PCR". En Genomics, Proteomics, and Clinical Bacteriology, 191–211. Totowa, NJ: Humana Press, 2004. http://dx.doi.org/10.1385/1-59259-763-7:191.
Texto completoEvrard, A., N. Boulle y G. s. Lutfalla. "Real-Time PCR". En Nanoscience, 841–69. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-88633-4_15.
Texto completoKonrad, Regina y Ulrich Busch. "PCR und Real-Time PCR". En Molekularbiologische Methoden in der Lebensmittelanalytik, 35–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10716-0_4.
Texto completoSluijter, J. P. G., G. Pasterkamp y D. P. V. de Kleijn. "Quantitative Real-Time PCR". En Cardiovascular Research, 75–83. Boston, MA: Springer US, 2006. http://dx.doi.org/10.1007/0-387-23329-6_4.
Texto completoBroll, Hermann. "Quantitative Real-Time PCR". En Molecular Biological and Immunological Techniques and Applications for Food Chemists, 59–83. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470637685.ch3.
Texto completoBartholomew, Rachel A., Janine R. Hutchison, Timothy M. Straub y Douglas R. Call. "PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification". En Manual of Environmental Microbiology, 2.3.2–1–2.3.2–13. Washington, DC, USA: ASM Press, 2015. http://dx.doi.org/10.1128/9781555818821.ch2.3.2.
Texto completoSchmittgen, Thomas D., Eun Joo Lee y Jinmai Jiang. "High-Throughput Real-Time PCR". En Methods in Molecular Biology, 89–98. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-040-3_7.
Texto completoDotti, Isabella, Ermanno Nardon, Danae Pracella y Serena Bonin. "Quantitative Real-Time RT-PCR". En Guidelines for Molecular Analysis in Archive Tissues, 121–32. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_25.
Texto completoActas de conferencias sobre el tema "Real-time PCR"
Becker, Holger, Nadine Hlawatsch, Richard Klemm, Christian Moche, Thomas Hansen-Hagge y Claudia Gärtner. "Real-time PCR in microfluidic devices". En SPIE MOEMS-MEMS, editado por Bonnie L. Gray y Holger Becker. SPIE, 2014. http://dx.doi.org/10.1117/12.2037241.
Texto completoWoudenberg, Timothy M. y J. Stevens. "Quantitative PCR by real-time detection". En Photonics West '96, editado por Gerald E. Cohn, Steven A. Soper y C. H. Winston Chen. SPIE, 1996. http://dx.doi.org/10.1117/12.237619.
Texto completoBARLOCCHI, G., U. MASTROMATTEO, S. SASSOLINI, M. SCURATI y F. VILLA. "MICROFLUIDIC DEVICE FOR REAL TIME PCR DETECTION". En Proceedings of the 9th Italian Conference. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812701770_0058.
Texto completoGANDELMAN, O. A., V. L. CHURCH, C. A. MOORE, C. CARNE, H. JALAL, J. A. H. MURRAY y L. C. TISI. "BART – BIOLUMINESCENT ALTERNATIVE TO REAL-TIME PCR". En Chemistry, Biology and Applications. WORLD SCIENTIFIC, 2007. http://dx.doi.org/10.1142/9789812770196_0023.
Texto completoChen, Ping-Hei, Da-Sheng Lee, Jui-Hung Chien y Meng-Hsun Wu. "Development of a Novel Real-Time PCR Machine". En European Conference on Biomedical Optics. Washington, D.C.: OSA, 2005. http://dx.doi.org/10.1364/ecbo.2005.wg7.
Texto completoKubista, Mikael, Anders Stalberg y Tzachi Bar. "Light-up-probe-based real-time Q-PCR". En BiOS 2001 The International Symposium on Biomedical Optics, editado por Ramesh Raghavachari y Weihong Tan. SPIE, 2001. http://dx.doi.org/10.1117/12.424589.
Texto completoHertwig, Aline Morgan von, Maristela da Silva do Nascimento, Maria Helena Pelegrinelli Fungaro y Marta Hiromi Taniwaki. "Real-Time Pcr for Identification of Aspergillus Niger". En XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-077.
Texto completoLee, D. S., J. H. Chien, M. H. Wu y P. H. Chen. "Development of a novel real-time PCR machine". En European Conference on Biomedical Optics 2005, editado por Christian D. Depeursinge. SPIE, 2005. http://dx.doi.org/10.1117/12.633056.
Texto completo"RqPCRAnalysis: Analysis of Quantitative Real-time PCR Data". En International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2013. http://dx.doi.org/10.5220/0004312002020211.
Texto completoGaertner, Claudia, Holger Becker, Nadine Hlawatsch, Richard Klemm, Christian Moche, René Sewart, Rainer Frank y Andreas Willems. "Lab-on-a-chip PCR: real time PCR in miniaturized format for HLA diagnostics". En SPIE Sensing Technology + Applications, editado por Brian M. Cullum y Eric S. McLamore. SPIE, 2014. http://dx.doi.org/10.1117/12.2050233.
Texto completoInformes sobre el tema "Real-time PCR"
Dilcheva, Valeria, Ivelin Vladov y Svetlozara Petkova. A Comparative Study of Six Trichinella Species by Real-time PCR Assay. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, enero de 2018. http://dx.doi.org/10.7546/crabs.2018.01.08.
Texto completoDilcheva, Valeria, Ivelin Vladov y Svetlozara Petkova. A Comparative Study of Six Trichinella Species by Real-time PCR Assay. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, enero de 2018. http://dx.doi.org/10.7546/grabs2018.1.08.
Texto completoHarris, D. L. Hank, Isabel Turney Harris, James S. Dickson, Stephen Gaul, Brad T. Bosworth y Lori Feldmann. Quantitative Real-time PCR (qPCR) for the Determination of Salmonella Levels in Lairage. Ames (Iowa): Iowa State University, enero de 2009. http://dx.doi.org/10.31274/ans_air-180814-1009.
Texto completoMcAvin, James C. y Carl J. Mason. Pre-Clinical Testing of a Real-Time PCR Assay for Diahhreal Disease Agent Cryptosporidium. Fort Belvoir, VA: Defense Technical Information Center, mayo de 2014. http://dx.doi.org/10.21236/ada600722.
Texto completoBonab, Zahra Hojjati, Parisa Mohammadi, Ezzat Asgarani y Nassim Ghorbanmehr. The Evaluation of Nitrogen Fixation Activity of Soil Cyanobacteria via Reduction Assay and Real-time PCR. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, junio de 2019. http://dx.doi.org/10.7546/crabs.2019.06.07.
Texto completoFaris, Gregory W. Development of Laser-Mediated Nanodroplet Real-Time PCR on Circulating Tumor Cells (CTC) by Microfilter Platform. Fort Belvoir, VA: Defense Technical Information Center, junio de 2015. http://dx.doi.org/10.21236/ada621341.
Texto completoMcAvin, James C. y Carl J. Mason. Norovirus Real Time RT-PCR Detection Technology Transition to the Joint Biological Identification and Diagnosis System (JBAIDS). Fort Belvoir, VA: Defense Technical Information Center, septiembre de 2012. http://dx.doi.org/10.21236/ada568257.
Texto completoMcAvin, James C. y Carl J. Mason. Pre-Clinical Testing of Real-Time PCR Assays for Diarrheal Disease Agents of Genera Escherichia and Shigella. Fort Belvoir, VA: Defense Technical Information Center, mayo de 2014. http://dx.doi.org/10.21236/ada600976.
Texto completoHutchison, Janine R., Gregory F. Piepel, Brett G. Amidan, Michael A. Sydor y Brooke L. Deatherage Kaiser. False Negative Rates of a Macrofoam-Swab Sampling Method with Low Surface Concentrations of Two Bacillus anthracis Surrogates via Real-Time PCR. Office of Scientific and Technical Information (OSTI), mayo de 2015. http://dx.doi.org/10.2172/1186982.
Texto completoHutchison, Janine R., Gregory F. Piepel, Brett G. Amidan, Michael A. Sydor y Brooke L. D. Kaiser. False Negative Rates of a Macrofoam-Swab Sampling Method with Low Surface Concentrations of Two Bacillus anthracis Surrogates via Real-Time PCR. Office of Scientific and Technical Information (OSTI), junio de 2016. http://dx.doi.org/10.2172/1260869.
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