Tesis sobre el tema "PV string"
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Elamri, Nuria Ali. "Strain diversity and identification of Pseudomonas syringae pv. maculicola and related pathovars". Thesis, University of the West of England, Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365189.
Texto completoSkandalis, Nikolaos. "Isolation and characterization of effector genes in Pseudomonas syringae pv. tomato strain DC3000". Thesis, Imperial College London, 2006. http://hdl.handle.net/10044/1/11913.
Texto completoRybak, Myrian Asucena. "Genetic determinants of host range specificity of the Wellington strain of Xanthomonas axonopodis pv. citri". [Gainesville, Fla.] : University of Florida, 2005. http://purl.fcla.edu/fcla/etd/UFE0011522.
Texto completoBaker, Christina Marie. "Strain typing and characterization of (sigma⁵⁴-) dependent transcriptional activator mutants in Pseudomonas syringae PV . tomato". Click HERE to connect, 2009. http://digital.library.okstate.edu/etd/Baker_okstate_0664D_10127.pdf.
Texto completoYu, Yanhua. "Functional characterization of AvrBs3/PthA effectors in Xanthomonas oryzae pv. oryzae strain BAI3 from West-Africa". Thesis, Montpellier 2, 2010. http://www.theses.fr/2010MON20231.
Texto completoXanthomonas oryzae pv. oryzae (Xoo) is the causal agent of Bacterial Leaf Blight (BLB) on rice, a serious disease causing important yield losses in the main rice growing regions including Africa. The virulence of Asian Xoo strains mainly depends on the type III effectors of avrBs3/pthA gene family, namely TAL (for Transcription Activator Like) effectors. In depth studies on the function of TAL effectors revealed that the virulence and/or the avirulence activities conferred by these effectors requires the binding and the induction of the corresponding S and/or R genes. African Xoo strains was shown to harbor 8 TAL effectors in their genomes. However, the contribution of these TAL effectors to Xoo virulence is still unknown. This work reports on the identification and characterization of TAL effectors in the African Xoo strain BAI3R. A random mutagenesis based on homologous recombination in the genes encoding TAL effector was conducted in Xoo str ain BAI3R and led to the identification of talC. TalC harbors 21.5 repeats in its central domain and is phylogenetically more related to TAL effectors of Xanthomonas oryzae pv. oryzicola (Xoc). The BAI3RΔtalC mutant is seriously impaired in its virulence on susceptible rice varieties. Interestingly, bacteria are still able to grow at wild-type levels in the apex of the leaf, suggesting a requirement of talc for vascular colonization. Potential direct host targets were identified by conducting a transcriptomic analysis of rice leaves challenged with Xoo strain BAI3R vs. BAI3RΔtalC. Among the identified targets, the rice gene Os11N3 was found to be highly induced upon infection by the wild type strain but not the mutant one. A DNA target box for TalC was located in the Os11N3 upstream region and proved to be functional using GUS assays. We also show that the Os11N3 341-bp upstream region is transcriptionnally activated by TalC. Our results demonstrated for the first time that TAL effectors play an important role in the virulence of Xoo strain BAI3R. Our work will contribute to better improve rice for resistance to bacterial leaf blight
Tudor, Simone Michelle. "Molecular characterization of bacteriocin-like activity in tomato race-three strains of Xanthomonas campestris pv. vesicatoria". [Gainesville, Fla.] : University of Florida, 1999. http://etd.fcla.edu/etd/uf/1999/amp7408/tudor.pdf.
Texto completoTitle from first page of PDF file. Document formatted into pages; contains vi, 121 p.; also contains graphics (some colored). Vita. Includes bibliographical references (p. 108-120).
Sahin, Fikrettin. "Detection, identification and characterization of strains of Xanthomonas campestris pv. vesicatoria by traditional and molecular methods, and resistance in Capsicum species to Xanthomonas campestris pv. vesicatoria pepper race 6 /". The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487945320758679.
Texto completoKang, Hyojeung. "Molecular analysis of secretion genes located on the syr-syp genomic island of Pseudomonas syringae pv. syringae strain B301D". Texas A&M University, 2004. http://hdl.handle.net/1969.1/1320.
Texto completoWonni, Issa. "Les bactérioses du riz dues à Xanthomonas oryzae au Burkina Faso : Diversité et identification de sources de résistance adaptées". Thesis, Montpellier 2, 2013. http://www.theses.fr/2013MON20174/document.
Texto completoBacterial Leaf Blight (BLB) and Bacterial Leaf Streak (BLS) diseases respectively caused by Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae. pv oryzicola (Xoc) are two emerging diseases of rice in West Africa, due to the recent expansion of rice cultivation and introduction of improved rice varieties over the last decade. Three news Xoo races were characterized based on genetic analysis, demonstrating their african specificity. In contrast, a study achevied on about ten Xoc strains isolated in Mali in 2003, show that they are related to asian Xoc strains. In Asia, several R genes against Xoo have been identified and deployed in breeding program to control BLB. In contrast, no R gene against Xoc has been identified in rice. The objectives of this PhD thesis are to (i) complete the Xo collections of African isolates upon annual sampling operated from 2009 to 2012 in various agroecological areas of Burkina Faso and Mali, (ii) determine the genetic diversity of these strains , (iii) identify and characterize news sources of resistance genes to BLB and BLS within rice accessions cultivated in Burkina Faso.Our results showed that african Xoc are highly diverse genetically and phenotypically. PCR-based analyse of two conserved type III effector gene (xopAJ and xopW) differentiated two groups of Xoc strains, with xopAJ not detected in a majority of African Xoc strains and 1050 bp insertion detected in xopW gene for few strains. However, the high genetic diversity observed among the Xoc strains is not correlated to geographical origin, sampling data or host plant species. Xoo strains characterized belong all to race A1 previously reported by Gonzalez et al. (2007) in Burkina Faso. Given the diversity of X. oryzae strains and their evolution, it is essential to establish a large scale epidemiological monitoring of Xo populations in concerned regions in west Africa.At last, some accessions cultivated in Burkina Faso showed specific resistance to african Xoo strains at all plant development stages. These original data contrast with rice lines carring Xa4, xa5 and Xa7 resistance genes against BLB, which are only effective at maximun tillering stage.Given no sources of effective resistance genes against BLS is available in rice, these accessions which were also efficient against a set of Xoc strains representative of the diversity in Africa, represent a huge potential source for the control of BLS in Burkina Faso, and eventually in others african countries
Appel, Maryke. "Cloning and identification of genes involved in the interaction between the bacterial stone fruit pathogen Pseudomonas syringae pv. syringae strain NV and plum trees". Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52165.
Texto completoENGLISH ABSTRACT: Bacterial canker of stone fruit, caused by Pseudomonas syringae pv. syringae, is one of the most destructive crop diseases in South Africa. Chemical control has failed completely and effective long-term management strategies will have to rely on the breeding of resistant host trees. To assist in such breeding programmes, investigations into the molecular basis of the interaction between P. s. pv. syringae and stone fruit trees have been undertaken in collaboration with the ARC-Fruit, Wine and Vine Research Institute in Stellenbosch. The aim of this dissertation was to clone and identify genes that are involved in interaction between the bacterial canker pathogen and stone fruit trees. In the first part of the study, the harpin encoding gene of a local strain of the pathogen, P. s. pv. syringae NV, was amplified in a polymerase chain reaction (PCR) strategy with primers based on the hrpAZB sequences of the bean pathogen, P. s. pv. syringae 61. Sequencing of this hrpZpssNvgene revealed a high degree of homology (96%) between the harpin encoding genes and harpin proteins of the two strains. The hrpZpssNvgene was subsequently cloned into the pMAL-c2 expression vector and expressed in Escherichia co/i. This system was used for the production of purified, biologically active, recombinant HrpZpSSNV protein. In the second part of the study, differential display (DD) technology was used to identify genes that are induced in stone fruit trees in response to P. s. pv. syringae and/or its harpin elicitor. For this purpose, actively growing shoots of two Prunus sa/icina cultivars, the moderately resistant cv. 'Laetitia' and the highly susceptible cv. 'Songold' were treated with recombinant harpinpssNvprotein or live P. s. pv. syringae NV bacteria. An untreated control and wounding control was included in the experiment. Total RNA was isolated for comparative mRNA analysis 24 hours after treatment. DD profiles were generated with fifteen primer combinations. Eight candidate bands were re-amplified, cloned and sequenced. Reverse transcription PCR was employed to verify the expression patterns of the cloned bands in the original RNA sample set. Two bands, DDc and DD4 were shown to be differentially expressed between treatments and/or cultivars, while no differences in the expression levels of the remaining six bands (DDa, DDe, DD3, DD5, DD6 and DD7) were observed. BLAST similarity searches yielded significant matches for DDe, DD4 and DD7 with plant defense-related genes.
AFRIKAANSE OPSOMMING: Bakteriese kanker van steenvrugte, wat deur Pseudomonas syringae pv. syringae veroorsaak word, is een van die mees verwoestende siektes van landbougewasse in Suid-Afrika. Chemiese beheermaatreëls het geheel en al misluk en effektiewe langtermyn beheerstrategieë sal op die teling van weerstandbiedende gasheerbome moet staatmaak. Ondersoeke na die molekulêre basis van die interaksie tussen P. s. pv. syringae en steenvrugbome is in samewerking met die LNR-Vrugte-, Wyn- en Wingerdnavorsingsinstituut in Stellenbosch van stapel gestuur om tot sulke telingsprogramme by te dra. Die doelwit van hierdie proefskrif was om gene wat betrokke is in die interaksie tussen die bakteriese kanker patogeen en steenvrugbome te kloneer en te identifiseer. In die eerste gedeelte van die studie is die harpien-koderende geen van 'n plaaslike ras van die patogeen, P. s. pv. syringae NV, geamplifiseer in 'n polimerase kettingreaksie (PKR)-strategie met peilers wat op die hrpAZB-geenopeenvolgings van die boontjiepatogeen, P. s. pv. syringae 61, gebaseer is. Volgordebepaling van hierdie hrpZpssNv-geen het 'n hoë vlak van homologie (96%) tussen die harpien-koderende gene en harpien proteïene van die twee rasse getoon. Die hrpZpssNv-geen is vervolgens in die uitdrukkingsvektor pMAL-c2 gekloneer en uitgedruk in Escherichia coli. Hierdie sisteem is vir die produksie van suiwer, biologies-aktiewe, rekombinante HrpZpssNv-proteingebruik. In die tweede gedeelte van die studie is die differensiaalvertoon (DD) tegniek gebruik om gene te identifiseer wat deur P. s. pv. syringae en/of sy harpien elisitar in steenvrugbome geïnduseer word. Vir hierdie doel is aktief-groeiende lote van twee Prunus sa/icina kultivars, die matig weerstandbiedende kv. 'Laetitia' en die hoogs vatbare kv. 'Songold', met rekombinante harpienpssNvproteïen of lewende P. s. pv. syringae NV bakterieë behandel. 'n Onbehandelde- en verwondingskontrole is in die eksperiment ingesluit. Totale RNA is 24 uur na behandeling vir vergelykende mRNA-analise geïsoleer. DD-profiele is met vyftien peilerkombinasies gegenereer. Agt kandidaatbande is geheramplifiseer en gekloneer, waarna hul DNA-opeenvolgings bepaal is. Trutranskriptase-PKR is gebruik om die ekspressiepatrone van die gekloneerde bande in die oorspronklike RNA monsters na te gaan. Daar is vasgestel dat twee van die bande, DDc en DD4, differensieel tussen kultivars en/of behandelings uitgedruk is, terwyl geen verskille in die ekspressievlakke van die oorblywende ses bande (DDa, DOe, 003, DOS, 006 en DO7) waargeneem is nie. BLAST-soektogte het betekenisvolle ooreenkomste vir DDe, DD4 en DD7 met plant weerstandsgeassosieerde gene opgelewer.
"High Power Density, High Efficiency Single Phase Transformer-less Photovoltaic String Inverters". Doctoral diss., 2017. http://hdl.handle.net/2286/R.I.45041.
Texto completoDissertation/Thesis
Doctoral Dissertation Electrical Engineering 2017
Li, Tsungchia y 李宗家. "Integration Of Two Maximum Power Point Trackers For Two-string PV Panels In A 5kW DC Distribution System". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/72306275847005788469.
Texto completo國立中正大學
電機工程研究所
100
This thesis presents development of two maximum power point trackers (MPPTs) for two-string PV panels in a 5 kW DC distribution system. To deal with wide output voltage range of the PV panels, the proposed MPPT topology consists of buck and boost converters. For increasing the efficiency of PV panels, a perturbation and observation algorithm is employed to track the maximum output power of the panels. Two MPPT modules and a bi-directional inverter connected with the AC grid are utilized to establish the DC distribution system. Moreover, the two maximum power point trackers can be operated in independent mode or in parallel mode. In the parallel mode, a current balancing control is adopted for the two MPPT modules. In addition, the two MPPT modules are designed with a hot-swap feature and power derating to increase the application feasibility of the two MPPT modules in the DC distribution system. Finally, experimental results have verified that the maximum power tracking accuracy can reach 99%.
"Substring Current-Voltage Measurement of PV Strings Using a Non-Contact I-V Curve Tracer". Master's thesis, 2020. http://hdl.handle.net/2286/R.I.57398.
Texto completoDissertation/Thesis
Masters Thesis Engineering 2020
"Application of Radiovoltmeters: Quick and Quantitative Power Determination of Individual PV Modules in a String without using I-V Curve Tracers". Master's thesis, 2019. http://hdl.handle.net/2286/R.I.55612.
Texto completoDissertation/Thesis
Masters Thesis Engineering 2019
Hsu, Shu-Ying y 許淑瑩. "Variability and grouping of strains of Xanthomonas campestris pv. vesicatoria from Taiwan". Thesis, 1998. http://ndltd.ncl.edu.tw/handle/35037181947520827298.
Texto completo國立中興大學
植物病理學系
86
The recent taxonomic studies showed that strains of Xanthomonascampestris pv. vesicatoria (Xcv) were heterogeneous population and weredivided into two genetically distinct groups (group A and group B). In orderto understand the variation and grouping of Xcv from Taiwan, a total of 152strains of Xcv from various localities of Taiwan were tested for theirphysiological, cell composition and molecular characteristics. Among the 152Xcv strains, 113 strains were nonpectolytic and nonamylolytic (group A) and 39strains were pectolytic and amylolytic (group B). The analysis by the systemof Biolog GN Microplate showed that strains varied greatly in utilization of anumber of carbon compounds. Cis-aconitate was the only substrate that helpedto discriminate between strains of the group A and group B. The fatty acidprofiles of 12 strains tested were qualitatively similar but quantitativedifferent. The amount of the 15:0 ante-iso fatty acid for group A strains(except XVT36) was lower than group B strains. Analyzing the whole-cellprotein patterns, strains of the same group were similar and which of thedifferent group were dissimilar. Particularly, the group A strains shared a32-33 kDa and a 24- 25 kDa protein band, whereas the group B strains sharedanother proteins with molecular weight about 26-27 kDa and 23-24 kDa. Based onthe cluster analysis of the protein profiles, though the strains of the samegroups were polymorphic, the similarity of the strains of the same group washigher than the strains of the different group. DNAs of Xcv strains weredigested with restriction enzymes Xba I and Spe I and analyzed by PFGE, theresult showed that the similarity of the DNA restriction fragments of the samegroup strains was higher than the strains of the different group. With theprimer pairs designed by previous investigators based on the DNA fragmentinvolved in the expression of two antigens associated with thelipopolysaccharide for reacting in PCR, all group A strains amplified a 560 bpproduct and group B strains did not. The homology of DNA of 5 group A strainsand 5 group B strains were analyzed by dot blot, the result showed that thesimilarity was 72~148 ﹪ for group A strains, 70~186 ﹪ for group B strainsand 12~58 ﹪ between group A and group B. The results of this study revealedthat Xcv strains from Taiwan were heterogeneous population, and can bedistinguished into two groups ( group A and group B ) which were the same asthat reported in other country. The group A belongs to Xanthomonas axonopodispv. vesicatoria and the group B was placed in Xanthomonas vesicatoria.
Xu, Shu-Ying y 許淑瑩. "Variability and grouping of strains of Xanthomonas campestris pv. vesicatoria from Taiwan". Thesis, 1998. http://ndltd.ncl.edu.tw/handle/35874935838082571626.
Texto completoSu, Yu-Chi y 蘇鈺琪. "Genotypic and Pathotypic Diversity of Xanthomonas oryzae pv. oryzicola Strains in Taiwan". Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107NCHU5363014%22.&searchmode=basic.
Texto completo國立中興大學
植物病理學系所
107
Bacterial leaf streak (BLS) disease of rice, elicited by Xanthomonas oryzae pv. oryzicola (Xoc), was originally discovered in the Philippines in 1918 and recently found in Taiwan (Nantou and Yulin counties) in 2007. To understand the diversity of the BLS pathogens in Taiwan, 48 Xoc strains isolated at different time and locations were differentiated by their pathological reactions on 18 rice varieties, i.e. IR24, 12 IR24-derived IRBB lines, and 5 Taiwan commercial lines, and by PCR-amplified DNA fingerprints that primed to repetitive sequences. The genotypes of Xoc strains were determined by BOX-PCR fingerprinting that grouped the 48 Xoc strains into 4 haplotypes. Rice inoculation tests in the fields and greenhouse showed the Xoc strains caused differential virulence on the 18 rice lines, and the rice cultivars IRBB5 and IRBB7 were more resistant to the majority of the 48 Xoc strains. Nevertheless, the bacterial haplotypes were not correlated with the geographical distribution or the pathological reactions on the rice cultivars. Additional rice inoculation tests on 2-month-old rice seedlings in the greenhouse confirmed that the rice resistance phenotypes were not affected by the inoculation methods (rubbing and infiltration) or the plant age. However, some rice cultivars, e.g. TK9, exhibited differential pathological reactions between greenhouse and field assays, suggesting the rice resistance against Xoc infection may be modulated by environmental factors. The results of this research revealed the genotypic and pathological diversity of Xoc strains in Taiwan, which can be applied to develop durable resistant cultivars to rice bacterial leaf streak disease.
p'in, wang mei y 王美蘋. "Phenotypeical study of fleQ mutant and complement strains in Xanthomonas campestris pv. campestris". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/26184195550551491945.
Texto completo亞洲大學
生物科技學系碩士班
95
Xanthomonas campestris pv. campestris (Xcc) is the pathogen causing black rot in cruciferous plants. It has previously demonstrated in our laboratory that a fleQ mutant is non-flagellated and immobile and expression of flagella genes fliE, fliQ, fliL, flgG, flgB, and flhF was depended on transcriptional activator, FleQ. In this work, it is shown that expression of fliA and fliC is also regulated by FleQ. In order to know if a plasmid-encoded fleQ gene can complement a mutation in fleQ, the fleQ gene was cloned into pBBad22K carrying an arabinose inducible promoter. The derived plasmid, pBadfleQ, was introduced into mutants and the parental strain. The phenotypic and Western blot analysis demonstrated that plasmid-encoded fleQ was expressed in the absence of inducer and the small amount of FleQ produced was sufficient to complement the mobility defect of a fleQ mutant. Addition of arabinose was increased the production of FleQ but decreased the effect of complementation. Induction to overexpress FleQ by 0.05% arabinose in Xc17fleQ::Gm (pBadfleQ ) and Xc17(pBadfleQ ) hindered their growth and severely reduced the biosynthesis of extracellular polysaccharide (EPS) and extracellular enzymes, such as amylases, proteinases, and pectinases. The promoter activity of the major EPS biosynthesis gene gumB is inhibited by the overexpressed FleQ protein. Since EPS and extracellular enzymes are important virulence factors in Xcc, Xc17fleQ::Gm(pBadfleQ) and Xc17(pBadfleQ), are less virulent than their parental wild-Type strain. It has previously been show in our laboratory that RpoN, FleQ, FleN, FlhF, FlgM and FliA can regulate flagellar genes expression. In this work, yeast two-hybrid system was used to further analyze the protein-protein interaction between these regulators. Our data revealed that proteins FleN and FliA and FlgM and FliA, RpoN2 and FlhF have interaction.
Shigaki, Toshiro. "Differential epidemiological fitness among strains of Xanthomonas campestris pv. campestris and the genetics of pathogenicity". Thesis, 1996. http://hdl.handle.net/10125/9465.
Texto completoXU, XIU-HUI y 許秀惠. "Characterization of strains of Xanthomonas campestris pv. vesicatoria from Taiwan and electron and light microscopy of tomato infected leaves". Thesis, 1989. http://ndltd.ncl.edu.tw/handle/50217862167803337907.
Texto completoNiemann, Nicolaas Johannes Jacobus. "Molecular characterisation of the causal agent of bacterial leaf streak of maize / Nicolaas Johannes Jacobus Niemann". Thesis, 2015. http://hdl.handle.net/10394/15478.
Texto completoMSc Environmental Sciences, North-West University, Potchefstroom Campus, 2015
Garton, Jeffrey Earl. "Evaluation of race and copper tolerant strains of Xanthomonas axonopodis pv. vesicatoria, causal agent of bacterial leaf spot of bell pepper in Georgia". 2009. http://purl.galileo.usg.edu/uga%5Fetd/garton%5Fjeffrey%5Fe%5F200912%5Fms.
Texto completo