Tesis sobre el tema "Pure culture"
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Porcu, Elisabetta. "Pure Land Buddhism in modern Japanese culture /". Leiden : Brill, 2008. http://catalogue.bnf.fr/ark:/12148/cb413311546.
Texto completoMAZURE, NATHALIE. "Etude de la biodegradation de la morphine par une culture pure mycobacterium aurum, et les cultures mixtes, pseudomonas". Compiègne, 1993. http://www.theses.fr/1993COMP648S.
Texto completoZarnegar, Abdolreza. "Purification and Characterization of an Inhibitor of Thymidine Uptake From Culture Supernatants of Human Tonsil Lymphocytes". Digital Commons @ East Tennessee State University, 1987. https://dc.etsu.edu/etd/2833.
Texto completoBrading, Melanie Gayle. "The influence of fluid dynamics and surface material on pure and binary culture biofilms". Thesis, University of Exeter, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307314.
Texto completoWang, Hao. "Development and electrochemical characterization of a Pseudomonas aeruginosa-based pure culture microbial fuel cell". University of Dayton / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1311958719.
Texto completoLamballe, Fabienne. "Reparation du dna dans les hepatocytes de rat adulte en culture pure et co-culture : mecanismes enzymatiques et expression d'oncogenes nucleaires". Rennes 1, 1989. http://www.theses.fr/1989REN10038.
Texto completoBally, Matthias. "Physiology and ecology of nitrilotriacetate degrading bacteria in pure culture, activated sludge and surface waters /". [S.l.] : [s.n.], 1994. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10821.
Texto completoQiu, Yu-Shan. "Development of a successful method for quantifying viable oral anaerobic spirochetes from pure culture and periodontal pockets". Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68246.
Texto completoThis method was extended to enumerate viable oral spirochetes from periodontal pockets. The antibiotic rifampin (20 $ mu$g/ml) was found to be an excellent selective agent for such a count when added to NOS-agarose medium. Counts of cultivable oral spirochetes from 10 subgingival plaque samples ranged from 12.5% to 28.2% of the total cultivable anaerobic bacteria. In addition, by the use of this method thirteen new oral spirochetes were isolated.
The viable count technique was modified and employed to study the locomotion of spirochetes. Migration of oral spirochetes out of NOS-Bacto agar medium into NOS-agarose medium was observed and two locomotory phenotypes of oral spirochetes were detected.
Kijkla, Pruch. "Biocide Mitigation of Carbon Steel and Stainless Steel Biocorrosion by Pure-Strain and Mixed-Culture Microbial Biofilms". Ohio University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1619007982435067.
Texto completoBertrand, François. "Les oidiums des cucurbitacees : maintien en culture pure, etude de leur variabilite et de la sensibilite chez le melon". Paris 11, 1991. http://www.theses.fr/1991PA112286.
Texto completoPatureau, Dominique. "Etudes cinétique et physiologique d'une bactérie dénitrifiant en conditions aérobies. Suivi en réacteur aéré, parfaitement mélangé, en culture pure et en culture mixte associée à une flore nitrifiante". Toulouse, INSA, 1995. http://www.theses.fr/1995ISAT0034.
Texto completoGriffiths, Jonathan T. H. "The effect of temperature upon the growth and metabolism of Aeromonas hydrophila and Lactobacillus plantarum in pure and mixed culture". Thesis, Oxford Brookes University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363793.
Texto completoLam, Melissa Karmen. "'The Pure Products of America Go Crazy' Defamiliarizing American Language and Culture in Lolita and The Crying of Lot 49". Thesis, University of Canterbury. English, 2006. http://hdl.handle.net/10092/942.
Texto completoPakdee-Patrakorn, Chusak. "Recherches sur les potentialités fourragères du soja (Glycine max. (L. ) Merrill) : en culture pure et en association avec le mai͏̈s". Toulouse, INPT, 1986. http://www.theses.fr/1986INPT012A.
Texto completoPakdee-Patrakorn, Chusak. "Recherches sur les potentialités fourragères du soja, Glycine max. (L.) Merrill, en culture pure et en association avec le maïs". Grenoble 2 : ANRT, 1986. http://catalogue.bnf.fr/ark:/12148/cb37600205n.
Texto completoPersson, Johan y Erik Eliason. "Ett schackbräde är inget utan sina pjäser : En beskrivande studie av användares deltagande hos nischade online pure players". Thesis, Linnéuniversitetet, Institutionen för medier och journalistik (MJ), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-45418.
Texto completoFRESSINAUD, MASDEFEIX CATHERINE. "Contribution a l'etude de la myelinisation in vitro : analyse des acides gras et etude ultrastructurale d'oligodendrocytes de rat myelinisant en culture pure". Limoges, 1988. http://www.theses.fr/1988LIMO0210.
Texto completoNeilson, James. "Attachment of bacteria to glass surfaces in pure culture and in mixed suspensions and the effect of growth conditions on that attachment". Thesis, University of Warwick, 1991. http://wrap.warwick.ac.uk/108883/.
Texto completoChahatha, Hisham El. "Étude de quelques aspects écologiques des relations nutritionnelles entre graminées et légumineuses en présence d'un champignon endomycorhizien en culture pure et mixte (compétition, symbiose, pollution)". Nancy 1, 1991. http://www.theses.fr/1991NAN10181.
Texto completoSurtiningsih, Suprapto Tini. "Mobilisation de P et CD de phosphates naturels par des bactéries et des champignons ectomycorhiziens en culture pure et dans la rhizosphère du pin sylvestre". Nancy 1, 1994. http://www.theses.fr/1994NAN10271.
Texto completoBouzar, Fatouma. "Variabilité clonale de Lactobacillus delbrueckii subsp. Bulgaricus CNRZ 1187 : influence sur la production des polysaccharides exocellulaires au cours de la fermentation en culture pure et mixte". ENSIA, 1997. http://www.theses.fr/1997EIAA0065.
Texto completoVu, Thi Thu Tra [Verfasser]. "Investigation of Vibrio and ESBL/AmpC-producing Enterobacteriaceae in retail seafood and inactivation of Vibrio in pure culture and mussel homogenates using high hydrostatic pressure / Thi Thu Tra Vu". Berlin : Freie Universität Berlin, 2019. http://d-nb.info/117715272X/34.
Texto completoSkandrani, Dalila. "Détermination des seuils de toxicité de divers insecticides (forme pure ou commerciale) sur cellules humaines en culture (A549, SH-SY5Y) : expression des gènes et protéines de stress (HSPs, GRPs,…)". Toulouse 3, 2006. http://www.theses.fr/2006TOU30233.
Texto completoToxicity of several insecticides was determined in vitro on lung adenocarcinoma A549 and neuroblastoma SH-SY5Y cell lines, with the aim to find out, among stress proteins, reliable and sensitive markers of occupational or accidental exposure. Carbamates (formétanate, methomyl, pyrimicarb), organochlorines (dienochlor, endosulfan), pyrethroid (bifenthrin) and neonicotinoid (imidacloprid) insecticides were comparatively investigated either as pure chemical or as commercial formulations. Measurement of threshold concentrations (LOEC) leading to a significant decrease of the growth-rate in A549 cells showed that organochlorines were the most toxic whereas imidacloprid and methomyl were the less toxic. SH-SY5Y cells were found to be more sensitive than A549. When compared at similar concentration of active principle, commercial formulations were found to be twice to 100 times more aggressive than the respective pure active molecule. In A549, GRP78 stress protein was up-regulated by almost all the insecticides, commercial formulations being more efficient. No such effect was observed in SH-SY5Y. Conversely, cytosolic HSP72/73 stress proteins were somewhat underexpressed in all cases. .
Grateau, Philippe. "Sensibilités, cultures et doléances : regard culturel sur les cahiers de doléances de 1789". Rennes 2, 1999. http://books.openedition.org/pur/23366.
Texto completoThe registers of grievances have been worked on, exploited and published. They have been widely commented upon especially by experts including Michelet,Taine, Tocqueville, Jaures or Furet. Since they have been subjected to all sorts of reading grids, ranging from cursive to structural reading, and searched for any relevant information they could disclose about the political, the economic and the cultural situation of the time, they seem to hold no more secrets. Yet, commemorating the bicentennial on a scientific level was the opportunity to remind every one of us of their extraordinary richness. Reading them from a cultural point of view isn't aimed at being considered as + scoop ;. It rather aims, on the one hand, at taking into account the immense historical production of the last three decades and on the other hand, at making the most of the product of the extraordinary effort which has been put into publication for over a century. Indeed, recent works in the field of public opinion or material culture raise new questions. Along the same hoes, the numerous available editions enable us to approach things from qualitative as well as a quantitative angle by examining them on different scales (local, regional, national). After presenting a critical synthesis of the existing works on the subject, the survey concerning both national and regional levels, casts a light on rural people's moral and philosophical aspirations, whether these convey their yearning for freedom, equality, happiness or progress. From these representations, it then goes on to explore cultural attitudes. For instance, through a complaint about the tax system, the members of the peasant community expressed their moral and philosophical yearnings, they described their conception of authority and sovereignty, they disclosed their fear of running short of bread, their dread of being struck by a disease or else their craving for culture. They wondered about the adequate means to collect the necessary funds for the education of their children, for medicine or to lure a capable surgeon and a competent midwife into settling in their village. Thus people of modest means cast a special light - at least in the context of a meeting - on what the ideals of the + siècle des Lumières ; were to lead on to on a local scale
Rohe, Lena [Verfasser], Reinhard [Akademischer Betreuer] Well, Nicole [Akademischer Betreuer] Wrage-Mönnig, Klaus [Akademischer Betreuer] Dittert y Heinz [Akademischer Betreuer] Flessa. "Nitrous oxide from fungal denitrification - Pure culture and soil studies using stable isotope and microbial inhibitor approaches / Lena Rohe. Gutachter: Nicole Wrage-Mönnig ; Klaus Dittert ; Heinz Flessa. Betreuer: Reinhard Well". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2014. http://d-nb.info/1064148298/34.
Texto completoGarnier-Petit, Agathe. "Purification et caractérisation de la glutamate déshydrogénase à NAD chez le basidiomycète ectomycorhizien Laccaria bicolor : régulation de son activité chez le champignon en culture pure et dans deux associations ectomycorhiziennes". Nancy 1, 1998. http://www.theses.fr/1998NAN10312.
Texto completoCottrell, Corey Ann. "Projeto Pure Mutt - Puro vira lata: Um Estudo Coreográfico nas Danças Urbanas: Samba-reggae, Capoeira e Hip Hop". reponame:Repositório Institucional da UFBA, 2007. http://www.repositorio.ufba.br/ri/handle/ri/9213.
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Esta dissertação tem o objetivo de demonstrar o processo da criação coreográfica inspirada em danças urbanas da diáspora africana. Na pesquisa coreográfica, foram trabalhadas as seguintes modalidades de dança: samba-reggae, capoeira e hip hop. Um dos principais objetivos foi o trabalho integrado com os artistas-participantes. O projeto realizou a apresentação de um trabalho de criação coletiva, que foi gravado na forma digital e editado em DVD, no qual revela toda a pesquisa e o seu processo criativo, contendo depoimentos dos participantes, clips dos ensaios e a performance final. Os resultados do projeto foram obtidos através da pesquisa bibliográfica e da pesquisa de campo. A pesquisa bibliográfica focalizou nas danças urbanas acima mencionadas, as teorias de cultura, etnicidade, cultura popular afro-brasileira e afro-americana, e a globalização. A pesquisa de campo tomou forma a partir de colaborações com a Escola de Dança da Fundação Cultural do Estado da Bahia e da Escola de Capoeira Ginga e Malícia, e com a minha participação como artista e espectadora em alguns eventos de samba-reggae, capoeira, hip hop e reggae, em Salvador, durante o período de 2005 a 2007. Na Escola de Dança (FUNCEB), Escola de Capoeira Ginga e Malícia e na Faculdade de Educação na UFBA foram realizados ensaios durante três meses, que produziram o produto criativo alvo desta pesquisa. A apresentação final foi realizada e gravada na Praça Dois de Julho, no Campo Grande, em Salvador - BA, em dezembro de 2006. Este trabalho também foi apresentado na 32a Conferência da Associação de Estudos Caribenhos em Salvador, em maio de 2007.
Salvador
Caris, Andrade Rodrigo. "Biodegration of commonly used plasticizers by pure bacterial cultures". Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=106606.
Texto completoL'adipate de di-(2-éthylhexyle), le dibenzoate de diéthylène-glycol et le dibenzoate de dipropylène-glycol sont des plastifiants d'usage commun identifiés comme contaminants environnementaux. Des études de biodégradation de ces plastifiants, effectuées avec la bactérie Rhodococcus rhodochrous et la levure Rhodotorula rubra en présence d'une source additionnelle de carbone, ont montré que ces molécules sont partiellement biodégradés. Cette biodégradation mène à la formation de métabolites toxiques, dont certains sont persistants. L'objectif de cette thèse de maitrise était de déterminer si le mécanisme de biodégradation et la nature des métabolites formés sont transférables à d'autres microorganismes et d'évaluer le rôle de la source additionnelle de carbone dans cette biodégradation. Pour ce faire, les plastifiants mentionnés ci-dessus ont été ajoutés à des cultures pures de différentes bactéries communément présente dans le sol, et ce, lorsque ces cultures ont atteint la phase stationnaire et que la source additionnelle de carbone, l'héxadécane, ait été totalement consommée. L'ensemble des bactéries étudiées a démontré un potentiel de biodégradation des plastifiants testés, et ce, même en absence d'une source additionnelle de carbone. Un niveau plus élevé de biodégradation a été observé dans le cas de l'adipate de (2-éthylhexyle) que dans ceux des dibenzoates. Les mécanismes de biodégradation observés ici en absence d'une source additionnelle de carbone sont comparables à ceux rapportés dans la littérature en présence d'héxadécane. Les métabolites formés étaient également les mêmes et bien que seulement une biodégradation partielle ait été observée, la formation des métabolites les plus récalcitrants et toxiques (2-éthylhexanol, acide 2-éthylhexanoïque, benzoate de diethylène glycol et benzoate de dipropylène glycol) a mené à une certaine accumulation.Cette thèse de maitrise démontre qu'une source additionnelle de carbone n'est pas nécessaire pour que la biodégradation de ces plastifiants ait lieu et que la biodégradabilité ainsi que les mécanismes de biodégradation publiés antérieurement ne sont pas spécifiques aux microorganismes Rhodococcus rhodochrous et Rhodotorula rubra et s'étendent plutôt à divers types commun de bactéries. Ces résultats suggèrent une biodégradation probable de ces plastifiants dans l'environnement.
Cronje, Marise Christine. "Production of kepi grains using pure cultures as starters". Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53561.
Texto completoENGLISH ABSTRACT: Kepi is a refreshing, fermented dairy beverage that differs from other fermented milk products in that it is produced with a mixed microbial community which is confined to discrete grains. These grains can be recovered as a solid matrix at the end of the fermentation and then be reutilised as a starter to ferment the next batch of milk. The grain microbial community consists of a symbiotic association of yeasts and lactic acid bacteria, but the overall composition of the grains has not been completely elucidated. The microbes in the grains are embedded in a protein-polysaccharide Kefiran matrix, which appears essential for grain formation. The mechanism of grain formation is still not fully understood and it thus remains undecided which organism is really responsible for the production of this proteinpolysaccharide matrix. The aim of this study was to isolate, characterise and identify the microbes present in Kefiran from mass cultured South African grains and then to evaluate grain formation with these purified cultures isolated from Kefiran strings using a mass cultivation process. Sixteen strains of lactic acid bacteria and one yeast strain were isolated from Kefiran strings produced during the mass cultivation of South African Kepi grains. API technology, numerical clustering and DNA sequence comparisons were used to identify the purified isolates. The isolates were grouped into seven clusters by numerical clustering and clustering distance from selected reference and marker strains. The heterofermentative lactobacilli were identified as Lactobacillus parakefiri and Lb. kefiri and the homofermentative strains as Lb. delbrueckii ssp. bulgaricus, Lb. gallina rum, Lb. acidophilus and Lb. bavaricus. One isolate was found to be a member of the genus Lactobacillus, but was not positively identified to species level. Cultures isolated from Kefiran were evaluated for ability to grain formation by adding 1 x 109 cfu.ml:' bacteria and 1 x 108 cfu.ml' yeast to double pasteurised, full cream milk during the mass cultivation process. It was found that the control and all the cultures in double pasteurised milk showed grain accumulation indicating that other microbes were present in pasteurised and double pasteurised milk which had an influence on the grain forming ability. The cultures isolated from pasteurised and double pasteurised milk included members of the species Pediococcus, Acinetobacter, Lactococcus laetis ssp. lactis, Candida lipolytica, C. guilliermondii, Chryseobacterium meningosepticum, Pseudomonas putida and four isolates of the Bacillus cereus group. It was found that these rod-shaped "milk isolates" resulted in grain accumulation when inoculated into UHT milk and it was concluded that the "milk isolates" did contribute to grain formation. These isolates were then combined with the Kefiran cultures and this resulted in grains very similar to the traditional Kepi grains. These grains were made from Lb. gallinarum in double pasteurised milk as well with a combination of Lb. gallinarum, Lb. acidophilus, Lb. kefiri, Lb. delbrueckii ssp. bulgaricus, Candida lambica and Pseudomonas putida in URT milk. The grains were firm, elastic and did not dissolve in water but kept their structure and were retained when sieved. An acceptable Kepi beverage was produced from these grains. From these typically traditional grain characteristics it was concluded that, even though the microbial compositions were probably not the same, the general appearance was similar to traditional grains and that it is thus possible to produce grains from pure single strain Kefiran cultures and "milk isolates". Furthermore, it was possible to produce a Kepilike beverage from these grains, which included similar characteristics as the traditional Kepi beverage.
AFRIKAANSE OPSOMMING: Kepi is "n verfrissende, gefermenteerde suiweldrankie wat van ander gefermenteerde produkte verskil in die opsig dat dit vervaardig word deur Kepi korrels in melk te inkubeer. Die Kepi korrels kan aan die einde van die fermentasie herwin word en weer gebruik word om die volgende lot melk te fermenteer. Die korrels bestaan uit "n simbiotiese samestelling van giste en melksuurbakterieë, maar die presiese samestelling van die korrels is steeds onbekend. Die mikro-organismes is vasgevang in "n proteïen-polisakkaried Kefiran matriks en die Kefiran word as essensieel beskou vir korrelvorming. Die meganisme van korrelvorming bly steeds onbekend en daar is nog nie tot "n gevolgtrekking gekom oor watter organisme die Kefiran produseerder is nie. Die doel van die studie was om die mikro-organismes in Kefiran te isoleer en te identifiseer deur Suid-Afrikaanse Kepi korrels te massa kweek. Hierdie mikroorganismes was dan verder geëvalueer ten opsigte van korrel vorming. Sestien melksuurbakterieë isolate en een gis isolaat is geïsoleer vanuit die Kefiran. API tegnologie, numeriese groepering en DNA volgorde vergelykings was gebruik om die isolate te identifiseer. Die isolate is in sewe groepe verdeel volgens numeriese groepering. Die afstand van verwysings en merker organismes is ook in ag geneem. Die heterofermentatiewe organismes is geïdentifiseer as Lactobacillus parakefiri en Lb. kefiri en die heterofermentatiewe organismes as Lb. delbrueckii ssp. bulgaricus, Lb. gallina rum, Lb. acidophilus en Lb. bavaricus. Een isolaat kon nie geïdentifiseer word tot op spesie vlak nie, maar is verwant aan die genus Lactobacillus. Hierdie geïsoleerde Kefiran kulture is geëvalueer ten op sigte van korrelvorming, deur 1 x 109 kve.ml' van die bakterieë en 1 x 108 kve.ml' van die gis by dubbel gepasteuriseerde volroom melk te voeg tydens die massakwekings proses. Die kontrole wat geen bygevoegde kulture bevat nie, sowel as die wat wel bygevoegde kulture bevat, het korrel vorming getoon. Laasgenoemde toon dat daar organismes teenwoordig is in gepasteuriseerde en dubbel gepasteuriseerde melk wat "n rol kan speel tydens korrelvorming. Die kulture wat geïsoleer is vanuit gepasteuriseerde en dubbel gepasteuriseerde melk, sluit in: Pediococcus, Acinetobacter, Lactococcus laetis ssp. lactis, Candida lipolytica, C. guilliennondii, Chryseobacterium menigosepticum, Pseudomonas putida en vier isolate van die Bacillus cereus groep. Hierdie organismes wat uit melk geïsoleer is, het korrelvorming getoon in UHT melk en die gevolgtrekking kan gemaak word dat die "melk organismes" wel "n rol speel tydens korrel vorming. Hierdie "melk isolate" in kombinasie met die Kefiran kulture het korrels tot gevolg gehad wat baie dieselfde was as tradisionele Kepi korrels. Laasgenoemde korrels is gemaak deur Lb. gallina rum in dubbel gepasteuriseerde melk, sowel as deur "n kombinasie van Lb. gallina rum, Lb. acidophilus, Lb. kefiri, Lb. delbrueckii ssp. bulgaricus, Candida lambica en Pseudomonas putida in UHT melk. Die korrels was stewig, elasties, het nie opgelos in water nie en het hulle struktuur behou wanneer gesif. Wanneer hierdie tipiese tradisionele korrels se eienskappe in ag geneem word, kan die gevolgtrekking gemaak word dat alhoewel die mikrobiese samestelling van die korrels nie dieselfde is as die tradisionele korrel nie, is die algemene voorkoms en eienskappe dieselfde en dat dit wel moontlik is om korrels te produseer deur isolate geïsoleer vanuit Kefiran en melk. Verder was dit moontlik om "n drankie te vervaardig met die korrels wat baie dieselfde is as tradisionele Kepi.
Besse, Laurent. "Les maisons des jeunes et de la culture : 1959-1981 : Etat, associations, municipalités". Paris 1, 2004. http://books.openedition.org/pur/4064.
Texto completoLyons, Reneé C. "Celebrate Hispanic Culture with Pura Belpré Award Winners". Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etsu-works/2378.
Texto completoKrammer, Thibault. "Développement d'un réseau microvasculaire sur puce microfluidique pour la reconstruction tissulaire". Thesis, Université Grenoble Alpes (ComUE), 2019. http://www.theses.fr/2019GREAV044.
Texto completoTissue engineering aims to develop functional tissues or organs in vitro in order to provide drug testing platforms or transplantable tissues and improve the treatments provided to patients. However, the physiological tissue structures developed to date do not integrate and perfusable vascular network. In vivo, the vascular network supplies the body’s cells with oxygen and nutrients and removes cellular waste and carbon dioxide. It also has a major role in maintaining organ homeostasis. Blood capillaries are hollow vessels whose walls are only composed of a layer of endothelial cells and diffuse nutrients. The blood capillary network is dense and perfuse all tissues. Due to the limit oxygen diffusion inside tissues, each cell is located at most 200µm away from a capillary. The difficulties of building a network of perfusable capillaries and integrating them into tissue constructs limit the development of thick physiological tissues.An innovative technique for developing a microvascular network within a thick construction is presented in this thesis. This technique consists of assembling spherical tissue micro-units within a microfluidic chamber, and developing a network of capillaries through the interstitial pores formed by the spheres packing. Tissue micro-units are composed of biopolymers representative of the extracellular matrix and contain cells from the tissue of interest. A layer of endothelial cells is developed on the surface of these microspheres. The stacking of these microspheres creates a porous medium in which nutrient medium is perfused. Flow control within such a structure allows the application of physical stimuli influencing the self-assembly of endothelial cells into capillaries within the interstitial space of the sphere packing.During this thesis, a device for manufacturing microspheres from natural biopolymers was developed. The structure formed by the stacks of spheres was studied and the flow within such environments were characterized so as to apply controlled physical stimuli to cells. A bioreactor-like perfusion system has been built. A thick tissue structure could be formed within this system and the development of the vascular network was promoted. The formation of the network was demonstrated by the presence of infused blood capillaries within the structure. The technique developed promises to be applied to the development of many tissues and applications for organ-on-chip or tissue engineering devices
Wilson, Craig Stewart. "The biodegradation of nitriles by pure and defined mixed cultures of bacteria". Thesis, University of Kent, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242890.
Texto completoChuang, Adina Shiang Mattes Timothy E. "Proteomic investigations of vinyl chloride-assimilating bacteria from pure cultures to the environment /". [Iowa City, Iowa] : University of Iowa, 2009. http://ir.uiowa.edu/etd/347.
Texto completoChuang, Adina Shiang. "Proteomic investigations of vinyl chloride-assimilating bacteria: from pure cultures to the environment". Diss., University of Iowa, 2009. https://ir.uiowa.edu/etd/347.
Texto completoBerthuy, Ophélie. "Puce à cellules multiplexée pour l'étude de réponses cellulaires parallélisées". Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10133/document.
Texto completoThe work reported in this thesis focuses on the development of a multiplexed cell chip for the study of parallelized cellular responses. The lineage of cells from Prostate cancer LNCaP cells, were used as a study model thanks to their ability to secrete prostate-specific antigen (PSA) and β-2-microglobulin (B2M) in response to induction by hormones such as dihydrotestosterone (DHT). We were able to detect in real time these label-free molecules and their secretion by small populations of adherent LNCaP cells (from 1 to 100 cells) at specified positions on a SPRi biochip. Three different approaches were considered for this biochip. The first was to pattern the gold surface of a SPRi slide to obtain microwells whose bottom reveals the gold surface (cytophilic area) and an outer shell composed of polystyrene (cytophobe) to create an adhesive/non-adhesive surface for cell culture. Antibodies were immobilized in a controlled manner in the microwells using a piezo electric spotter. In this miniaturized system, different cell lines were co-cultured on a surface of 1 cm², paving the way for multiplexing. A small population of cells (1 to 100) was deposited in an automated manner into each microwell. In order to maintain the cells in a hydrated environment during deposition, a biocompatible alginate polymer was used. This method allows the encapsulation of cells in a very small volume (<50 nL). The ability of the hydrogel to maintain the encapsulated cells in a given position on the support led to the design of a second approach for the production of the biochip. In this second approach the surface is not altered and biological compounds (antibodies and cells) are directly deposited in an automated manner on the gold layer. Finally, a last approach was developed by immobilizing the cells on a patterned substrate placed in front of the sensitive layer SPRi. In all three approaches, the kinetics of PSA secretion and secreted B2M could be followed by SPRi
Dastidar, Aniruddha. "ARSENITE OXIDATION BY PURE CULTURES OF THIOMONAS ARSENIVORANS STRAIN B6 IN BIOREACTOR SYSTEMS". UKnowledge, 2010. http://uknowledge.uky.edu/gradschool_diss/70.
Texto completoEstevam, Andressa. "Obtenção de BIOH 2 a partir da fermentação anaeróbia de efluente de cervejaria utilizando cultura pura isolada do ambiente". Universidade Estadual do Oeste do Paraná, 2017. http://tede.unioeste.br/handle/tede/3009.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The search for alternative fuels to replace the current demand for fossil fuels is growing worldwide, as is the case of the use of biomass for the production of clean energy. H2, besides being an important input in the industrial sector, is an excellent fuel due to its calorific value and the non-generation of gaseous pollutants. The production of hydrogen by anaerobic fermentation has been highlighted as an important route of production because it allows the use of residual substrates by microorganisms producing H2. Such microorganisms can be found in many sources in the form of anaerobic microbial consortia, for use in the form of mixed culture or for isolation of species. This work aimed at the isolation of bacteria from the environment, with the capacity to produce hydrogen by anaerobic fermentation of different substrates and the application of these cultures to the production of H2 using as a source of carbon the brewery effluent. Strains of bacteria isolated from avian litter were characterized and tested for potential H2 production in small scale trials, in static bottles and different substrates, in mesophilic condition. From the selection of a strain of better performance, the experiments were carried out in a mechanically stirred reactor, in a batch of 72 hours and using exclusively the brewery effluent. The H2 production occurred in all the tests and the average biogas flow rate was 72 mL h-1, H2 H2 molar flow rate of 1.2 mmol H2 h-1 and H2 content in the biogas of 30 to 40%, being this compound only by CO2 and H2.
A busca por combustíveis alternativos para substituir a demanda atual de combustíveis fósseis vem crescendo mundialmente, como é o caso da utilização de biomassa para a produção de energia limpa. O H2, além de ser importante insumo no setor industrial é um excelente combustível devido a seu poder calorífico e à não geração de gases poluentes. A produção de hidrogênio por fermentação anaeróbia vem se destacando como uma importante rota de produção pois permite a utilização de substratos residuais por micro-organismos produtores de H2. Tais micro-organismos podem ser encontrados em muitas fontes na forma de consórcios microbianos anaeróbios, para uso na forma de cultura mista ou para isolamento de espécies. Este trabalho teve como objetivo o isolamento de bactérias do ambiente, com capacidade de produzir hidrogênio por fermentação anaeróbia de diferentes substratos e a aplicação destas culturas à produção de H2 utilizando como fonte de carbono o efluente de cervejaria. Cepas de bactérias isoladas de cama de aviário foram caracterizadas e testadas quanto ao potencial de produção de H2 em ensaios de pequena escala, em frascos estáticos e diferentes substratos, em condição mesofílica. A partir da seleção de uma cepa de melhor performance foram conduzidos ensaios em reator agitado mecanicamente, em batelada com duração de 72 horas e utilizando como fonte de carbono efluente de cervejaria, exclusivamente. A produção de H2 ocorreu em todos os ensaios sendo obtidos os valores médios de vazão de biogás de 72 mL h-1, vazão molar de H2 de 1,2 mmol H2 h-1 e teor de H2 no biogás de 30 a 40%, sendo este composto somente por CO2 e H2. PALAVRAS-
Ibarra, Barrera María Carolina. "Plan de regeneración pueblo de Putre". Tesis, Universidad de Chile, 2013. http://www.repositorio.uchile.cl/handle/2250/116281.
Texto completoLa presente memoria constituye la síntesis de los conceptos abordados para el desarrollo del proyecto de título definido como “Plan de Regeneración poblado de Putre”, realizado durante el período académico 2012-2013. Durante estos ultimos años conceptos como Cultura, Identidad y Memoria han sido los principales gestores al momento de idear y construir nuevas formas de hacer y pensar la arquitectura. la oportunidad para desarrollar teóricamente esta linea de pensamiento converge durante el 5º año de carrera, principalmente durante la etapa de seminario, el cual llevó por nombre “Reconstrucción de un Paisaje Cultural”, es en este momento donde logre comprender que estos conceptos toman medida solo en su estrecha relacion con el territorio. Asumiendo que los procesos son diversos y nunca totalmente acabados, el proyecto presente simplemente esboza aquellas ideas, intenciones, intereses y compromisos que de modo personal me interesa desarrollar en el futuro. A continuación graficare los principales conceptos abordados para la elaboración del presente proyecto.
Mosteller, Tracy M. "Sanitizer efficacy towards attached bacteria in a simulated milk pipeline system using pure and mixed cultures". Diss., This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-08062007-094410/.
Texto completoLaplace, Jean-Marie. "Cultures microbiennes pures et mixtes : production d'éthanol à partir des pentoses et des héxoses dérivés des lignocelluloses". Montpellier 2, 1992. http://www.theses.fr/1992MON20155.
Texto completoBaudoin, Régis. "Développement et caractérisation d'une puce à cellules pour le criblage d'agents toxiques". Compiègne, 2008. http://www.theses.fr/2008COMP1785.
Texto completoCurrent developments in tissue engineering and microtechnology fields allowed proposing new pertinent tools to investigate in vitro toxicity. We propose the development of a cellular microchip that mimics organs in vitro. To validate our approach, we showed the pertinence of the microchip environment with the culture of a renal cell line, the Madin Darby Canine Kidney (MDCK) and a human hepatic cell line, the HepG2/C3A. In this study, we tested three flow rates (0, 10 and 25 µL/min) at three inoculated cell densities. Then, we tested our microchip with three ammonium chloride loadings (0, 5 and 10mM) in order to demonstrate its potential for future toxicity experiments. The cellular activities were monitored by the cell proliferation rates, the glucose and glutamine consumptions, albumin and ammonia productions and by the detoxication via the CYP 1A activity. In dynamic condition, the cellular activities in term of consumptions and productions were higher in the microchip than in static conditions. More especially, the detoxication activity of the CYP 1A was found higher. The toxicity analysis with the chloride ammonium showed similar tendencies in the microchip and in Petri standard culture conditions (reduction of proliferation. However, the ammonium chloride seemed induce a higher CYP 1A activity in the microchip. By these investigations, we showed the pertinence of the utilization of our microchip for in vitro dynamic toxicity testing. The targeted industries of this new in vitro cell culture model are the chemical, pharmaceutical and cosmetic industries
MARIN, DOUGLAS. "Ecophysiologie de canavalia ensiformis (l. )dc. , en cultures pures et associees". Rennes 1, 1994. http://www.theses.fr/1994REN10196.
Texto completoBarbosa, Willer Araujo. "Cultura Purí e educação popular no município de Araponga, Minas Gerais". Florianópolis, SC, 2005. http://repositorio.ufsc.br/handle/123456789/102780.
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Análise do processo variável da emergência étnica no entorno do Parque Estadual da Serra do Brigadeiro na Zona da Mata de Minas Gerais. A Cultura Puri foi silenciada durante os últimos duzentos anos, a partir da Educação Popular, seu processo intercultural possibilitou a recriação de identidades. Através de entrevistas, de rodas de narração de histórias e das notas de campo em comunidades rurais recupera fontes orais. Capta um encadeamento discursivo recorrente entre violência, fabulações naturalísticas e vontade de liberdade. Se inscreve na tradição da crítica cultural como motor da transformação social. Associa-se à saga colonial migratória, e recoloca a temática dos movimentos sociais. Critica o monoculturalismo do saber através da inteligibilidade dos movimentos sociais e da ecologia de saberes. Enfatiza a constituição de uma outra temporalidade e da dilatação do tempo presente, o que aprofunda as análises de longa duração. Aborda conceitos que se interrelacionam complexamente, tais como: estrutura de sentimentos, hegemonia, residual e emergencial. Toma como referências os estudos de Raymond Williams e de Boaventura de Sousa Santos.
Ferreira, Maria de Fatima. "Melancolia: da identificação narcísica à pura cultura da pulsão de morte". Universidade Federal de Minas Gerais, 2006. http://hdl.handle.net/1843/VCSA-6X3REP.
Texto completoMarques, Nara Eliza. "De Chocolat : um Griot à brasileira: o mais puro cacau da Bahia contando histórias na República do Café com Leite /". Araraquara, 2020. http://hdl.handle.net/11449/192553.
Texto completoResumo: O final do século XIX e o começo do século XX é um período de intensa e diversa produção cultural decorrente das sucessivas mudanças que aconteciam não só no Brasil, mas em todo o mundo. Havia um processo de construção e afirmação de identidades e de reconstrução de hierarquias sociais que haviam ruído com a abolição do escravismo. Muitos desses movimentos culturais, principalmente os de agência de negros libertos, sofreram sucessivas tentativas de apagamento por terem caráter popular, ou seja, não se enquadrarem em um modelo europeu-ocidental reformador de costumes. É de um desses movimentos, mais especificamente de uma figura atuante na cena cultural do início do século XX que essa pesquisa irá erguer a voz. O nome artístico dele era De Chocolat, fundador da primeira companhia de teatro do Brasil composta apenas por negros, a Companhia Negra de Revista. Ele produz um teatro com uma importante função social. Utiliza-se de ferramentas populares como a música, a oralidade, a dança, os improvisos, os chistes, as anedotas para fazer críticas à sociedade da época. Essa dissertação terá como elemento central o fac-símile da primeira peça encenada por De Chocolat e sua companhia, intitulada “Tudo Preto”. É através desse material que será visto, que assim como um griot africano – que são intermediadores sociais e guardadores das histórias de seu povo – De Chocolat usará as populares Revistas Teatrais para contar a história da construção da identidade negra no Brasil tangenciando ass... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The end of the nineteenth century and the beginning of the twentieth century is a period of intense and diverse cultural production resulting from successive changes that took place not only in Brazil, but also around the world. There was a process of building and affirming identities and rebuilding social hierarchies that had broken with the abolition of slavery. Many of these cultural movements, especially those of freed black agencies, have suffered successive attempts to erase because they are popular in nature that is not fitting into a Western-European customs reformer. It is from one of these movements, more specifically of an active figure in the cultural scene of the early twentieth century that this research will raise the voice. His stage name was De Chocolat, founder of Brazil's first black theater company, Black Company of Magazines. He produces a theater with an important social function. It uses popular tools such as music, orality, dance, improvisations, jokes, anecdotes to criticize the society of the time. This dissertation will have as its central element the facsimile of the first play staged by De Chocolat and his company, entitled "All black". It is through this material that he will be seen, as well as an African griot – who are social mediators and keepers of the stories of his people – De Chocolat will use the popular Theatre of Magazines to tell the story of the construction of black identity in Brazil such as black women, racism, religiosity, Brazil... (Complete abstract click electronic access below)
Mestre
Vadelorge, Loïc. "Pour une histoire culturelle du local : Rouen, 1919-1940". Paris 4, 1996. http://books.openedition.org/pur/10987.
Texto completoIf french cultural history is today well known, the local culture development is still a matter of investigation. The case of Rouen, one of the most important provincial cities during the french twentieth century, suggest that local cultural practices have been in existence for a long time. The local culture during the third republic took one's stand on a complete system of cultural institutions (museums, libraries, theaters) and used all the resources of associations (scientist or musical societies). The birth of cultural policies, who compete with traditionnal forms of cultural organisations (clerical, elites) reinfor ce the local culture. It appears then able to resist to the economics mutations (inflation during the twenties, great crisis in 1929) or cultural mutations (the age of the masses). The patrimonial sense is here decisive as it result s of two impulsions, local and national. The overture of cultural sense, during the french popular front, will not be able to change the politics and the practices ; this is the sign of the extraordinary permanence of the local cultural system in the early twentieth century france
Ratau, Mmaphuti Abashone. "Chemometrics, physicochemical and sensory characteristics of pearl millet beverage produced with bioburden lactic acid bacteria pure cultures". Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2773.
Texto completoThe aim of this study was to evaluate the physical, chemical and sensory characteristics of non-alcoholic pearl millet beverage produced using isolated and purified cultures of bioburden lactic acid bacteria (LAB). Traditional non-alcoholic pearl millet beverage (TNAPMB) was produced through spontaneous fermentation. The slurry was fermented for 36 h at 37°C while monitoring the microbial growth at 3 h interval. LAB were grown on deMan, Rogosa and Sharpe agar and identified using Vitek 2 system. The initial numbers of LAB were 7.04 log cfu/ml and increased to 8.00 log cfu/ml after 21 h. The beverage was dominated by LAB and contaminants and their survival was in succession. LAB from the genera Leuconostoc, Pediococcus, Streptococcus and Enterococcus were the main fermenting species in TNAPMB. Pearl millet extract (PME) was produced by hydrating pearl millet flour (PMF) with water (1:10, PMF:Water). To the mixture sprouted rice flour (10%), ground ginger (10%) and pectin (0.6%) were added. Stable PME was used in the production of plain non-alcoholic pearl millet beverage (PNAPMB). PME was pasteurized at 98°C for 30 min, hot filled and cooled to 25°C. The fluid was inoculated with Leuconostoc mesenteroides, Pediococcus pentosaceus and Enterococcus gallinarum each at 0.05, 0.075 and 0.1%, respectively, using factorial design and fermented for 18 h at 37°C. The pH of the beverage ranged between pH 3.32 and pH 3.90. L. mesenteroides, P. pentosaceus, E. gallinarum, the interaction between L. mesenteroides and P. pentosaceus and the interaction between L. mesentoroides and E. gallinarum had a significant effect (p ˂ 0.05) on the pH of PNAPMB except the interaction between P. pentosaceus and E. gallinarum (p = 0.631). The total titratable acidity (TTA) of the beverage ranged from 0.50 to 0.72%. All cultures had a significant influence (p ˂ 0.05) on the TTA of the beverage with the exception of the interaction between L. mesenteroides and E. gallinarum (p = 0.102). However, Monte Carlo simulation showed that E. gallinarum caused an increase in the pH and a decrease in the TTA of the beverage. During fermentation, the pH of the beverage is desired to decrease while the TTA increases, hence E. gallinarum was removed. The interaction between L. mesenteroides and P. pentosaceus at 0.05% and 0.025%, respectively produced an acceptable PNAPMB with potential for commercialization. Furthermore, moringa supplemented non-alcoholic pearl millet beverage (MSNAPMB) was produced by adding 4% of moringa (Moringa oleifera) leaf powder extract during the production of PNAPMB. The physicochemical, nutritional, microbial (LAB) and sensory characteristics of the PNAPMB, MSNAPMB and TNAPMB were determined. LAB were significantly (p < 0.05) affected by the fermentation period and increased from 3.32 to 7.97 log cfu/ml and 3.58 to 8.38 log cfu/ml in PNAPMB and SNAPMB, respectively. The pH of PNAPMB decreased from pH 5.05 to pH 4.14 while the pH of MSNAPMB decreased from pH 5.05 to pH 3.65 during the 18 h fermentation. The growth of LAB during fermentation had a significant effect (p < 0.05) on the pH of the beverages. The TTA increased from 0.14 to 0.22% and increased from 0.17 to 0.38%, in PNAPMB and MSNAPMB, respectively. The TTA of the beverage was affected significantly (p < 0.05) by the 18 h of fermentation. The protein content was 1.62, 2.17 and 1.50% in PNAPMB, MSNAPMB and TNAPMB, respectively. PNAPMB sample was deemed acceptable in comparison to the MSNAPMB. The total colour difference (ΔE) was 5.91 and 10.60 in PNAPMB and MSNAPMB, respectively in comparison to the TNAPMB. Volatile compounds with beneficial effect such as anti-inflammatory and anti-pathogenic properties were identified in the beverages. Principal component analysis indicated that the variations in characteristics of PNAPMB and MSNAPMB could be explained using total fat, saturated fat, total sugar, ash, moisture, proteins, chroma (C), hue and b*. The results showed that isolated pure cultures could be used as starter cultures in the production of non-alcoholic cereal beverages at a commercial level with predictable quality and safety properties.
Gage, Sue-Je Lee. "Pure mixed blood the multiple identities of Amerasians in South Korea /". [Bloomington, Ind.] : Indiana University, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3253643.
Texto completoTitle from PDF t.p. (viewed Nov. 19, 2008). Source: Dissertation Abstracts International, Volume: 68-02, Section: A, page: 0619. Adviser: Anya Peterson Royce.
Platz, Teresa Katharina. "Café culture : socio-historical transformations of space, personhood and middle class in Pune, India". Thesis, Durham University, 2012. http://etheses.dur.ac.uk/4461/.
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