Artículos de revistas: "Pseudomonas fluorescens"

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Gopal, Surendra, Reshma Francis y A. K. Sreelatha. "Impact of soil temperature, pH and carbon dioxide on the population and efficiency of fluorescent pseudomonad in the rhizosphere soil of Pokkali rice". Environment Conservation Journal 24, n.º 1 (8 de enero de 2023): 163–70. http://dx.doi.org/10.36953/ecj.10262239.

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The present study was aimed at the evaluation of soil temperature, pH and carbon dioxide evolution on the number and efficiency of fluorescent pseudomonads around the root system of Pokkali rice at Vytilla in Ernakulam district of Kerala. Two plots (40 m2) comprising control (without application of Pseudomonas fluorescens) and P. fluorescens treated plants were used for the field experiment. The isolates of fluorescent Pseudomonads or Pseudomonas fluorescence were counted and their efficiency was assessed for IAA, ammonia, HCN and siderophore production. Simultaneously, soil temperature, pH, and carbon dioxide evolution were also recorded. A total of 6 fluorescent pseudomonads (VPJU, VPJL, VPAU1, VPAU2, VPAU3 and VPAU4) were found during the crop period. All the isolates produced IAA and ammonia with varying degrees of intensity. Three isolates (VPAU1, VPAU3 and VPAU4) produced HCN, and no microbial isolates produced siderophore. The effect of soil temperature, pH, EC and carbon dioxide evolution was correlated with the number of fluorescent pseudomonads in the soil. The bacteria were significantly afflicted by pH and EC, whereas soil temperature and CO2 evolution did not show any effect on the number of fluorescent pseudomonads. There was no significant influence of soil temperature, pH, EC and carbon dioxide evolution on indole acetic acid production, ammonia, and HCN production. Inoculated Pseudomonas fluorescence did not survive in Pokkali rice fields. However, further studies are needed for at least three seasons in Pokkali soils to confirm the results of the present study.

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FARRAG, SEHAM A. y ELMER H. MARTH. "Behavior of Listeria monocytogenes when Incubated Together with Pseudomonas Species in Tryptose Broth at 7 and 13°C". Journal of Food Protection 52, n.º 8 (1 de agosto de 1989): 536–39. http://dx.doi.org/10.4315/0362-028x-52.8.536.

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Tryptose broth (TB) was inoculated with Listeria monocytogenes (strain Scott A or California), Pseudomonas aeruginosa, Pseudomonas flourescens, or a combination of L. monocytogenes plus Pseudomonas species, and incubated at 7 or 13°C for 8 weeks. McBride Listeria Agar was used to determine numbers of L. monocytogenes and Pseudomonas Isolation Agar to enumerate Pseudomonas species at 0, 7, 14, 28, 42, or 56 d. At 13°C, presence of P. fluorescens had a slight negative effect on growth of L. monocytogenes strain Scott A, and was somewhat detrimental to its survival during the extended incubation. Growth of L. monocytogenes strain California was retarded by presence of P. fluorescens although the maximum population achieved by the pathogen was greater in the presence rather than absence of the pseudomonad; the pseudomonad did have a negative effect on survival of the pathogen. At the same temperature, P. aeruginosa had a negative effect on survival of L. monocytogenes strain California, but had essentially no effect on the other strain of the pathogen. Neither strain of L. monocytogenes affected growth of P. fluorescens nor P. aeruginosa. At 13°C the pH of TB generally decreased when L. monocytogenes grew by itself but increased when either pseudomonad grew by itself or together with the pathogen. At 7°C, growth of both pseudomonads was minimal. Presence of non-growing cells of P. fluorescens retarded somewhat growth of both L. monocytogenes strains early during the incubation. P. aeruginosa had no detectable effect on either strain of L. monocytogenes. The pH of TB decreased when L. monocytogenes grew by itself or together with either pseudomonad, and remained unchanged in TB inoculated with either pseudomonad.

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Tryfinopoulou, P., E. Tsakalidou y G. J. E. Nychas. "Characterization of Pseudomonas spp. Associated with Spoilage of Gilt-Head Sea Bream Stored under Various Conditions". Applied and Environmental Microbiology 68, n.º 1 (enero de 2002): 65–72. http://dx.doi.org/10.1128/aem.68.1.65-72.2002.

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ABSTRACT The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolated from S. aurata stored under different temperatures (at 0, 10, and 20°C) and packaging conditions (air and a modified atmosphere of 40% CO2-30% N2-30% O2). Pseudomonas lundensis was the predominant species, followed by Pseudomonas fluorescens, while Pseudomonas fragi and Pseudomonas putida were detected less frequently. Fluorescent Pseudomonas strains dominated under air conditions, while proteolytic and less lipolytic strains dominated under modified-atmosphere packaging. Different storage conditions appear to govern the selection of pseudomonads in gilt-head sea bream fish.

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Velusamy, Palaniyandi, J. Ebenezar Immanuel, Samuel S. Gnanamanickam y Linda Thomashow. "Biological control of rice bacterial blight by plant-associated bacteria producing 2,4-diacetylphloroglucinol". Canadian Journal of Microbiology 52, n.º 1 (1 de enero de 2006): 56–65. http://dx.doi.org/10.1139/w05-106.

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Certain plant-associated strains of fluorescent Pseudomonas spp. are known to produce the antimicrobial antibiotic 2,4-diacetylphloroglucinol (DAPG). It has antibacterial, antifungal, antiviral, and antihelminthic properties and has played a significant role in the biological control of tobacco, wheat, and sugar beet diseases. It has never been reported from India and has not been implicated in the biological suppression of a major disease of the rice crop. Here, we report that a subpopulation of 27 strains of plant-associated Pseudomonas fluorescens screened in a batch of 278 strains of fluorescent pseudomonads produced DAPG. The DAPG production was detected by a PCR-based screening method that used primers Phl2a and Phl2b and amplified a 745-bp fragment characteristic of DAPG. HPLC,1H NMR, and IR analyses provided further evidence for its production. We report also that this compound inhibited the growth of the devastating rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae in laboratory assays and suppressed rice bacterial blight up to 59%–64% in net-house and field experiments. Tn5 mutants defective in DAPG production (Phl–) of P. fluorescens PTB 9 were much less effective in their suppression of rice bacterial blight.Key words: biocontrol, 2,4-diacetylphloroglucinol, Pseudomonas fluorescens, rice, Xanthomonas oryzae pv. oryzae.

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Aloysius Ng. Lende, Laurensius Lehar y Heny MC Sine. "Application of organic fertilizer and Pseudomonas fluorescens on the growth and yield of shallot cultivar Sabu Raijua (Allium ascalonicum L .) in dry land". GSC Advanced Research and Reviews 5, n.º 2 (30 de noviembre de 2020): 123–30. http://dx.doi.org/10.30574/gscarr.2020.5.2.0105.

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The specific objectives of this study were 1 ) knowing certain types of organic fertilizers on the growth of shallots 2 ) knowing the concentrations of Pseudomonas fluorescenss certain the growth of shallots, 3 ) knowing the types of organic fertilizers and the concentrations Pseudomonas fluorescens specificity increase the optimal yield of shallots. To achieve this goal, this research was conducted using factorial experiments with a split Plot Design with 10 treatments and 3 replications. So that there are 10 treatment combinations of a total number of 30 experimental plots. There were 2 factors that were tried, namely the first factor of Organic Fertilizer as the main plot, namely: cow manure 10 ton ha-1 (K1), chicken manure 10 ton ha-1 (K2). While the second factor as the subplot is the concentration of Pseudomonas fluorescens: Watering with water (as a control) 100 ml (P0), Watering with a concentration of Pseudomonas fluorescens 5 ml + normal water 95 ml (P1), Watering with a concentration of Pseudomonas fluorescens 10 + ordinary water 90 ml (P2), sprinkling with a concentration of Pseudomonas fluorescens 15 ml + 85 ml plain water (P3), Flushing with a concentration of Pseudomonas fluorescens 20 + ordinary water 80 ml (P4). The shallot cultivar of Sabu Raijua which was given organic fertilizer of 10 tonnes of chicken manure. Ha-1 and a concentration of Pseudomonas fluorescens 20 + 80 ml of plain water gave the highest growth component at the age of 10 WAP, namely at the age of 10 WAP, namely plant height (37.667cm). Leaves (34, 800 trees), number of tillers (10, 533 trees). The results of shallot bulbs of Sabu Raijua cultivar from organic fertilizer treatment of 10 ton ha-chicken manure1s with a concentration of Pseudomonas fluorescens 20 ml + 80 ml water resulted in components, namely tuber weight per plot (276.70 g ), number of tubers per plot (291, 70 tubers ).

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6

Armarkar, Sarika A., R. M. Gade y Mina D. Koche. "Growth Promotion Activity And Growth Pattern Of Pseudomonas Fluorescens On Different Solid Media". Journal of Plant Disease Sciences 17, n.º 1 (9 de agosto de 2022): 22–27. http://dx.doi.org/10.48165/jpds.2022.1706.

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Study was conducted in vitro to study the growth promotion activity and growth pattern of Pseudomonas fluorescens on different solid media. Soil samples were collected randomly from rhizosphere of citrus plants for isolation of Pseudomonas. Twenty six isolates was isolated, out of twenty six isolates eight isolates showed abiliity of siderophore production, thirteen isolates showed positiveness for IAA production, nine isolates showed phosphate solubilization and seven isolates were positive for HCN production. For growth pattern study of P. fluorescens three different media i.e. King’s B, Pseudomonas agar and nutrient agar used. All the isolates showed fast growth on King’s B followed by Pseudomonas agar and nutrient agar. Mostly Pseudomonas fluorescens produced greenish yellow coloured colonies on King’s B and Pseudomonas agar and dull yellowish coloured colony and creamy white coloured colony on nutrient agar. Fluorescent pigmentation was observed on King’B and Pseudomonas agar medium.

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7

Gottlieb, Tom, Glenn Funnell y Iain Gosbell. "Pseudomonas fluorescens pseudobacteraemia". Medical Journal of Australia 155, n.º 11-12 (diciembre de 1991): 854–55. http://dx.doi.org/10.5694/j.1326-5377.1991.tb94085.x.

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8

Snopková, Kateřina, Kristýna Dufková y David Šmajs. "Pseudomonas prosekii isolated in Antarctica inhibits plantpathogenic strains of Pseudomonas viridiflava and Pseudomonas fluorescens". Czech Polar Reports 11, n.º 2 (11 de febrero de 2022): 270–78. http://dx.doi.org/10.5817/cpr2021-2-18.

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Pseudomonas-caused plant diseases are present worldwide and affect most of the major lineages of higher plants which, as a consequence, may result in significant economic losses. Despite the use of bacteriocins produced by rhizosphere and soil bacteria has been nowadays considered as novel crop protection approach, antagonistic interactions of cold-adapted isolates toward agriculturally important phytopathogenic bacteria have not been studied yet. In this study, we tested inhibition activity of Antarctic Pseudomonas spp. against phytopathogenic pseudomonads. Four Antarctic stains (P. prosekii CCM 8878, CCM 8879, and CCM 8881 and Pseudomonas sp. CCM 8880) inhibited several phytopathogenic strains of P. viridiflava and P. fluorescens. Based on inhibition zone character and previous genome research we suggest that L-pyocin activity was responsible for this effect against P. viridiflava strains and that tailocin inhibited P. fluorescens isolate.

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9

Moënne-Loccoz, Yvan, Hans-Volker Tichy, Anne O'Donnell, Reinhard Simon y Fergal O'Gara. "Impact of 2,4-Diacetylphloroglucinol-Producing Biocontrol StrainPseudomonas fluorescens F113 on Intraspecific Diversity of Resident Culturable Fluorescent Pseudomonads Associated with the Roots of Field-Grown Sugar Beet Seedlings". Applied and Environmental Microbiology 67, n.º 8 (1 de agosto de 2001): 3418–25. http://dx.doi.org/10.1128/aem.67.8.3418-3425.2001.

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ABSTRACT The impact of the 2,4-diacetylphloroglucinol-producing biocontrol agent Pseudomonas fluorescens F113Rif on the diversity of the resident community of culturable fluorescent pseudomonads associated with the roots of field-grown sugar beet seedlings was evaluated. At 19 days after sowing, the seed inoculant F113Rif had replaced some of the resident culturable fluorescent pseudomonads at the rhizoplane but had no effect on the number of these bacteria in the rhizosphere. A total of 498 isolates of resident fluorescent pseudomonads were obtained and characterized by molecular means at the level of broad phylogenetic groups (by amplified ribosomal DNA restriction analysis) and at the strain level (with random amplified polymorphic DNA markers) as well as phenotypically (55 physiological tests). The introduced pseudomonad induced a major shift in the composition of the resident culturable fluorescentPseudomonas community, as the percentage of rhizoplane isolates capable of growing on three carbon substrates (erythritol, adonitol, and l-tryptophan) not assimilated by the inoculant was increased from less than 10% to more than 40%. However, the pseudomonads selected did not display enhanced resistance to 2,4-diacetylphloroglucinol. The shift in the resident populations, which was spatially limited to the surface of the root (i.e., the rhizoplane), took place without affecting the relative proportions of phylogenetic groups or the high level of strain diversity of the resident culturable fluorescent Pseudomonas community. These results suggest that the root-associatedPseudomonas community of sugar beet seedlings is resilient to the perturbation that may be caused by a taxonomically related inoculant.

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MARSHALL, DOUGLAS L. y RONALD H. SCHMIDT. "Growth of Listeria monocytogenes at 10°C in Milk Preincubated with Selected Pseudomonads1". Journal of Food Protection 51, n.º 4 (1 de abril de 1988): 277–82. http://dx.doi.org/10.4315/0362-028x-51.4.277.

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Preliminary studies involving co-inoculation of Listeria monocytogenes with Pseudomonas fragi into whole or skim milk demonstrated that neither inhibition nor stimulation of growth occurred for either organism. Additional investigations involved preincubation of whole milk, skim milk, and 10% reconstituted nonfat dry milk (NDM) for 3 d at 10°C with P. fragi, Pseudomonas fluorescens P26, P. fluorescens T25, or P. fluorescens B52, followed by inoculation with L. monocytogenes and further incubation at 10°C. Growth curves of L. monocytogenes were constructed for each treatment combination and generation times were statistically compared for differences. Results indicated that L. monocytogenes did not affect growth or survival of the preincubated Pseudomonas spp. However, growth rates of L. monocytogenes were significantly (P<0.05) enhanced in milks preincubated with pseudomonads. Doubling times of L. monocytogenes were reduced by up to 3 h when grown in milk preincubated with Pseudomonas spp. Three strains of P. fluorescens showed more stimulation of the growth rate of L. monocytogenes compared to P. fragi in preincubated whole or skim milk but not in preincubated NDM. Milk composition had little effect on growth of either genus when incubated alone. Results of this study indicate that L. monocytogenes can grow in the presence of Pseudomonas spp. either as a co-inoculant or following preincubation in milk at 10°C. Furthermore, data suggest that the presence of the pseudomonads may enhance growth of L. monocytogenes in milk.

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Godfrey, S. A. C., S. A. Harrow, J. W. Marshall y J. D. Klena. "Characterization by 16S rRNA Sequence Analysis of Pseudomonads Causing Blotch Disease of Cultivated Agaricus bisporus". Applied and Environmental Microbiology 67, n.º 9 (1 de septiembre de 2001): 4316–23. http://dx.doi.org/10.1128/aem.67.9.4316-4323.2001.

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ABSTRACT Bacterial blotch of Agaricus bisporus has typically been identified as being caused by either Pseudomonas tolaasii (brown blotch) or Pseudomonas gingeri(ginger blotch). To address the relatedness of pseudomonads able to induce blotch, a pilot study was initiated in which pseudomonads were selectively isolated from mushroom farms throughout New Zealand. Thirty-three pseudomonad isolates were identified as being capable of causing different degrees of discoloration (separable into nine categories) of A. bisporus tissue in a bioassay. These isolates were also identified as unique using repetitive extragenic palindromic PCR and biochemical analysis. Relationships between these 33 blotch-causing organisms (BCO) and a further 22 selected pseudomonad species were inferred by phylogenetic analyses of near-full-length 16S rRNA gene nucleotide sequences. The 33 BCO isolates were observed to be distributed throughout thePseudomonas fluorescens intrageneric cluster. These results show that in addition to known BCO (P. tolaasii, P. gingeri, and Pseudomonas reactans), a number of diverse pseudomonad species also have the ability to cause blotch diseases with various discolorations. Furthermore, observation of ginger blotch discoloration of A. bisporus being independently caused by many different pseudomonad species impacts on the homogeneity and classification of the previously described P. gingeri.

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Davis, Edward W., Rachel A. Okrent, Viola A. Manning y Kristin M. Trippe. "Unexpected distribution of the 4-formylaminooxyvinylglycine (FVG) biosynthetic pathway in Pseudomonas and beyond". PLOS ONE 16, n.º 4 (23 de abril de 2021): e0247348. http://dx.doi.org/10.1371/journal.pone.0247348.

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The biological herbicide and antibiotic 4-formylaminooxyvinylglycine (FVG) was originally isolated from several rhizosphere-associated strains of Pseudomonas fluorescens. Biosynthesis of FVG is dependent on the gvg biosynthetic gene cluster in P. fluorescens. In this investigation, we used comparative genomics to identify strains with the genetic potential to produce FVG due to presence of a gvg gene cluster. These strains primarily belong to two groups of Pseudomonas, P. fluorescens and P. syringae, however, a few strains with the gvg cluster were found outside of Pseudomonas. Mass spectrometry confirmed that all tested strains of the P. fluorescens species group produced FVG. However, P. syringae strains did not produce FVG under standard conditions. Several lines of evidence regarding the transmission of the gvg cluster including a robust phylogenetic analysis suggest that it was introduced multiple times through horizontal gene transfer within the Pseudomonas lineage as well as in select lineages of Thiomonas, Burkholderia and Pantoea. Together, these data broaden our understanding of the evolution and diversity of FVG biosynthesis. In the course of this investigation, additional gene clusters containing only a subset of the genes required to produce FVG were identified in a broad range of bacteria, including many non-pseudomonads.

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FARRAG, SEHAM A. y ELMER H. MARTH. "Growth of Listeria monocytogenes in the Presence of Pseudomonas fluorescens at 7 or 13°C in Skim Milk". Journal of Food Protection 52, n.º 12 (1 de diciembre de 1989): 852–55. http://dx.doi.org/10.4315/0362-028x-52.12.852.

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Autoclaved samples of skim milk were inoculated with Listeria monocytogenes (strain Scott A, California or V7), Pseudomonas fluorescens (strain P26 or B52), or a combination of L. monocytogenes plus P. fluorescens, and incubated at 7 or 13°C for 8 weeks. McBride Listeria Agar was used to determine populations of L. monocytogenes (at 0, 7, 14, 28, 42, or 56 d), and Pseudomonas isolation agar to enumerate P. fluorescens. Growth of L. monocytogenes was somewhat enhanced after 7 d of incubation at 7 but not at 13°C in the presence of pseudomonads. However, after 14 d and until the end of the incubation period (56 d), slight inactivation of L. monocytogenes in the presence of P. fluorescens was observed. L. monocytogenes did not affect growth or survival of P. fluorescens; also, no marked changes in pH of the milk were caused either by L. monocytogenes alone or by L. monocytogenes plus P. fluorescens.

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14

Gangwar, Gokil Prasad y A. P. Sinha. "Effect of fungal and bacterial bioagent application on total phenolic content in rice leaves pre-inoculated with Xanthomonas oryzae pv. oryzae (Uyeda and Ishiyama) Dowson". Journal of Applied and Natural Science 6, n.º 1 (1 de junio de 2014): 254–57. http://dx.doi.org/10.31018/jans.v6i1.410.

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Present study was carried out to observe the effect of fungal and bacterial bioagents on total phenolic content in rice leaves pre-inoculated with Xanthomonas oryzae pv. oryzae and on disease severity of bacterial leaf blight of rice. Two commercial formulations of Trichoderma harzianum (PBA-1) and Pseudomonas fluorescens (PBA-2) and four formulations of fluorescent pseudomonads and Trichoderma spp. viz, P. fluorescens (Pf 83, rice leaf isolate), fluorescent pseudomonad (FLP 88, rice leaf isolate), T. harzianum (rice leaf isolate), Trichoderma spp. (isolate 40, isolated from rice field soil) were evaluated. Significantly higher mean value of total phenolic content of rice leaves was observed with the application of bioagent formulations as compared to check (pre-inoculated with X. oryzae pv. oryzae), chemical treatment and healthy plant. Maximum mean total phenolic content (342.22 μl/g) in rice leaves was observed with Pf 83, which was followed by PBA-2 (334.44 μl/g) and T. harzianum (330.00 μl/g). Decrease in disease severity of bacterial leaf blight was observed with the increase of total phenolic content in rice leaves which resulted in increased grain yield and 1000 grain weight.

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15

Anderson, Mark y Richard Davey. "Pseudobacteraemia with Pseudomonas fluorescens". Medical Journal of Australia 160, n.º 4 (febrero de 1994): 233–34. http://dx.doi.org/10.5694/j.1326-5377.1994.tb126622.x.

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Meyer, Jean-Marie, Valérie A. Geoffroy, Nader Baida, Louis Gardan, Daniel Izard, Philippe Lemanceau, Wafa Achouak y Norberto J. Palleroni. "Siderophore Typing, a Powerful Tool for the Identification of Fluorescent and Nonfluorescent Pseudomonads". Applied and Environmental Microbiology 68, n.º 6 (junio de 2002): 2745–53. http://dx.doi.org/10.1128/aem.68.6.2745-2753.2002.

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ABSTRACT A total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. As a general rule, strains within a well-circumscribed taxonomic group, namely the species Pseudomonas brassicacearum, Pseudomonas fuscovaginae, Pseudomonas jessenii, Pseudomonas mandelii, Pseudomonas monteilii, “Pseudomonas mosselii,” “Pseudomonas palleronii,” Pseudomonas rhodesiae, “Pseudomonas salomonii,” Pseudomonas syringae, Pseudomonas thivervalensis, Pseudomonas tolaasii, and Pseudomonas veronii and the genomospecies FP1, FP2, and FP3 produced an identical pyoverdine which, in addition, was characteristic of the group, since it was structurally different from the pyoverdines produced by the other groups. In contrast, 28 strains belonging to the notoriously heterogeneous Pseudomonas fluorescens species were characterized by great heterogeneity at the pyoverdine level. The study of 23 partially characterized phenotypic clusters demonstrated that siderotyping is very useful in suggesting correlations between clusters and well-defined species and in detecting misclassified individual strains, as verified by DNA-DNA hybridization. The usefulness of siderotyping as a determinative tool was extended to the nonfluorescent species Pseudomonas corrugata, Pseudomonas frederiksbergensis, Pseudomonas graminis, and Pseudomonas plecoglossicida, which were seen to have an identical species-specific siderophore system and thus were easily differentiated from one another. Thus, the fast, accurate, and easy-to-perform siderotyping method compares favorably with the usual phenotypic and genomic methods presently necessary for accurate identification of pseudomonads at the species level.

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Silva, Gildo Almeida da y Erik Amazonas de Almeida. "Production of yellow-green fluorescent pigment by Pseudomonas fluorescens". Brazilian Archives of Biology and Technology 49, n.º 3 (mayo de 2006): 411–19. http://dx.doi.org/10.1590/s1516-89132006000400009.

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A medium was prepared from brewery waste yeast with and without mineral salts to study growth and yellow-green fluorescent pigment production (YGFP) by Pseudomonas fluorescens. The King's medium used for detection of siderophore production were expressively weaker inductors of YGFP formation when compared to FYE medium. Although FYE and CYE could be used for growth of P. fluorescens, only FYE was an attractive medium for detection of YGFP strain producers.

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R, Vimala y Suriachandraselvan M. "INFLUENCE OF ANTAGONISTIC AGENT, PLANT PRODUCTS AND CHEMICAL AGENTS ON THE POWDERY MILDEW DISEASE OF BHENDI AND ITS PRODUCTION". Journal of Biopesticides 1, n.º 2 (1 de diciembre de 2008): 130–33. http://dx.doi.org/10.57182/jbiopestic.1.2.130-133.

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Field investigations were made to study the influence of integrated disease management involving plant products and biological control agents of powdery mildew of Bhendi (Erysiphe cichoracearum DC) with ten treatments viz.,Pseudomonas fluorescens I18 (0.2%), P.fluorescens 1(0.2%), Ocimum sanctum 10%, Neem Seed Kernel Extract 5%, K2 HPO4 50 mM, Salicylic acid 1mM, O. sanctum 5% + P. fluorescens I18 (0.2%),Neem Seed Kernel Extract 5 % + P.fluorescens I18 (0.2%), Carbendazim 0.1% and Control. Two sprays were given; first one on 30 days after sowing and the second one on 60 days after sowing. Powdery mildew disease severity was recorded at weekly interval after the second spray on randomly selected plants based on 0-5 grade scale and PDI was calculated. At the end of the season, yield was recorded in each plot. The minimum disease incidence of 8.83% was recorded with Neem Seed Kernel Extract 5%+ Pseudomonas fluorescens I18 0.2% followed by 9.49% with Pseudomonas fluorescens I18 0.2%,10.36% with carbendazim and 11.9% with Pseudomonas fluorescens -1.The yield was also increased to the tune of 43.43% over control in NSKE 5%+Pseudomonas fluorescens I18 followed by 42.44% in Pseudomonas fluorescens I18 ,41.84% with 0.1% carbendazim and 39.73% with Pseudomonas fluorescens -1.

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Gandraburova, N. I., E. I. Kharina y A. G. Gadzhiahmedova. "EFFECTS OF PSEUDOMONAS FLUORESCENS ON SOME SOIL MICROORGANISMS". Nauka v sovremennom mire 37, n.º 4 (2019): 13–17. http://dx.doi.org/10.31618/2524-0935-2019-37-4-13-17.

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Inoue, Hiroyuki, Osamu Takimura, Hiroyuki Fuse, Katsuji Murakami, Kazuo Kamimura y Yukiho Yamaoka. "Degradation of Triphenyltin by a Fluorescent Pseudomonad". Applied and Environmental Microbiology 66, n.º 8 (1 de agosto de 2000): 3492–98. http://dx.doi.org/10.1128/aem.66.8.3492-3498.2000.

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ABSTRACT Triphenyltin (TPT)-degrading bacteria were screened by a simple technique using a post-column high-performance liquid chromatography using 3,3′,4′,7-tetrahydroxyflavone as a post-column reagent for determination of TPT and its metabolite, diphenyltin (DPT). An isolated strain, strain CNR15, was identified as Pseudomonas chlororaphis on the basis of its morphological and biochemical features. The incubation of strain CNR15 in a medium containing glycerol, succinate, and 130 μM TPT resulted in the rapid degradation of TPT and the accumulation of approximately 40 μM DPT as the only metabolite after 48 h. The culture supernatants of strain CNR15, grown with or without TPT, exhibited a TPT degradation activity, whereas the resting cells were not capable of degrading TPT. TPT was stoichiometrically degraded to DPT by the solid-phase extract of the culture supernatant, and benzene was detected as another degradation product. We found that the TPT degradation was catalyzed by low-molecular-mass substances (approximately 1,000 Da) in the extract, termed the TPT-degrading factor. The other fluorescent pseudomonads,P. chlororaphis ATCC 9446, Pseudomonas fluorescens ATCC 13525, and Pseudomonas aeruginosaATCC 15692, also showed TPT degradation activity similar to strain CNR15 in the solid-phase extracts of their culture supernatants. These results suggest that the extracellular low-molecular-mass substance that is universally produced by the fluorescent pseudomonad could function as a potent catalyst to cometabolite TPT in the environment.

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YOON, Hong Mook, Sung Hoon MOON y Young Hwan SONG. "Characterization of Biosurfactant Produced by Pseudomonas fluorescens PD101". Korean Journal of Fisheries and Aquatic Sciences 36, n.º 3 (1 de junio de 2003): 230–38. http://dx.doi.org/10.5657/kfas.2003.36.3.230.

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FREEDMAN, DANIEL J., JEFFERY K. KONDO y DOUGLAS L. WILLRETT. "Antagonism of Foodborne Bacteria by Pseudomonas spp.: A Possible Role for Iron1". Journal of Food Protection 52, n.º 7 (1 de julio de 1989): 484–89. http://dx.doi.org/10.4315/0362-028x-52.7.484.

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Antagonistic action of Pseudomonas spp. against foodborne spoilage and pathogenic bacteria was studied to determine mechanisms involved in the establishment of dominance by these organisms in food systems. Thirteen Pseudomonas strains from plant and food origin were tested for the ability to inhibit other Pseudomonas spp. on brain heart infusion agar using a bacteriocin screening assay. P. aeruginosa AA was the most active, inhibiting P. phaseolicola, P. pisi, P. putida, P. fluorescens, and P. fragi strains. When testing for antagonism against non-pseudomonads, four Pseudomonas spp. showed wide spectrum activity against a variety of gram positive and gram negative bacteria. Growth of most Pseudomonas spp. on an iron-deficient medium increased antagonism. In assays conducted in liquid media, inhibitory activity varied greatly as a result of the composition of the media. A minimal media extract from a P. aeruginosa AA culture significantly inhibited the growth of P. fluorescens and Staphylococcus aureus in milk.

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Mazzola, Mark, David M. Granatstein, Don C. Elfving, Kent Mullinix y Yu-Huan Gu. "Cultural Management of Microbial Community Structure to Enhance Growth of Apple in Replant Soils". Phytopathology® 92, n.º 12 (diciembre de 2002): 1363–66. http://dx.doi.org/10.1094/phyto.2002.92.12.1363.

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Apple replant disease typically is managed through pre-plant application of broad-spectrum soil fumigants including methyl bromide. The impending loss or restricted use of soil fumigants and the needs of an expanding organic tree fruit industry necessitate the development of alternative control measures. The microbial community resident in a wheat field soil was shown to suppress components of the microbial complex that incites apple replant disease. Pseudomonas putida was the primary fluorescent pseudomonad recovered from suppressive soil, whereas Pseudomonas fluorescens bv. III was dominant in a conducive soil; the latter developed within 3 years of orchard establishment at the same site. In greenhouse studies, cultivation of wheat in replant orchard soils prior to planting apple suppressed disease development. Disease suppression was induced in a wheat cultivar-specific manner. Wheat cultivars that enhanced apple seedling growth altered the dominant fluorescent pseudo-monad from Pseudomonas fluorescens bv. III to Pseudomonas putida. The microbial community resident in replant orchard soils after growing wheat also was suppressive to an introduced isolate of Rhizoctonia solani anastomosis group 5, which causes root rot of apple. Incorporation of high glucosinolate containing rapeseed (‘Dwarf Essex’) meal also enhanced growth of apple in replant soils through suppression of Rhizoc-tonia spp., Cylindrocarpon spp., and Pratylenchus penetrans. Integration of these methods will require knowledge of the impact of the biofumigant component on the wheat-induced disease-suppressive microbial community. Implementation of these control strategies for management of apple replant disease awaits confirmation from ongoing field validation trials.

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Couillerot, Olivier, Emeline Combes-Meynet, Joël F. Pothier, Floriant Bellvert, Elita Challita, Marie-Andrée Poirier, René Rohr, Gilles Comte, Yvan Moënne-Loccoz y Claire Prigent-Combaret. "The role of the antimicrobial compound 2,4-diacetylphloroglucinol in the impact of biocontrol Pseudomonas fluorescens F113 on Azospirillum brasilense phytostimulators". Microbiology 157, n.º 6 (1 de junio de 2011): 1694–705. http://dx.doi.org/10.1099/mic.0.043943-0.

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Pseudomonads producing the antimicrobial metabolite 2,4-diacetylphloroglucinol (Phl) can control soil-borne phytopathogens, but their impact on other plant-beneficial bacteria remains poorly documented. Here, the effects of synthetic Phl and Phl+ Pseudomonas fluorescens F113 on Azospirillum brasilense phytostimulators were investigated. Most A. brasilense strains were moderately sensitive to Phl. In vitro, Phl induced accumulation of carotenoids and poly-β-hydroxybutyrate-like granules, cytoplasmic membrane damage and growth inhibition in A. brasilense Cd. Experiments with P. fluorescens F113 and a Phl− mutant indicated that Phl production ability contributed to in vitro growth inhibition of A. brasilense Cd and Sp245. Under gnotobiotic conditions, each of the three strains, P. fluorescens F113 and A. brasilense Cd and Sp245, stimulated wheat growth. Co-inoculation of A. brasilense Sp245 and Pseudomonas resulted in the same level of phytostimulation as in single inoculations, whereas it abolished phytostimulation when A. brasilense Cd was used. Pseudomonas Phl production ability resulted in lower Azospirillum cell numbers per root system (based on colony counts) and restricted microscale root colonization of neighbouring Azospirillum cells (based on confocal microscopy), regardless of the A. brasilense strain used. Therefore, this work establishes that Phl+ pseudomonads have the potential to interfere with A. brasilense phytostimulators on roots and with their plant growth promotion capacity.

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Mavrodi, Dmitri V., Robert F. Bonsall, Shannon M. Delaney, Marilyn J. Soule, Greg Phillips y Linda S. Thomashow. "Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from Pseudomonas aeruginosa PAO1". Journal of Bacteriology 183, n.º 21 (1 de noviembre de 2001): 6454–65. http://dx.doi.org/10.1128/jb.183.21.6454-6465.2001.

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ABSTRACT Two seven-gene phenazine biosynthetic loci were cloned fromPseudomonas aeruginosa PAO1. The operons, designatedphzA1B1C1D1E1F1G1 and phzA2B2C2D2E2F2G2, are homologous to previously studied phenazine biosynthetic operons from Pseudomonas fluorescens and Pseudomonas aureofaciens. Functional studies of phenazine-nonproducing strains of fluorescent pseudomonads indicated that each of the biosynthetic operons from P. aeruginosa is sufficient for production of a single compound, phenazine-1-carboxylic acid (PCA). Subsequent conversion of PCA to pyocyanin is mediated in P. aeruginosa by two novel phenazine-modifying genes,phzM and phzS, which encode putative phenazine-specific methyltransferase and flavin-containing monooxygenase, respectively. Expression of phzS alone inEscherichia coli or in enzymes, pyocyanin-nonproducingP. fluorescens resulted in conversion of PCA to 1-hydroxyphenazine. P. aeruginosa with insertionally inactivated phzM or phzS developed pyocyanin-deficient phenotypes. A third phenazine-modifying gene,phzH, which has a homologue in Pseudomonas chlororaphis, also was identified and was shown to control synthesis of phenazine-1-carboxamide from PCA in P. aeruginosa PAO1. Our results suggest that there is a complex pyocyanin biosynthetic pathway in P. aeruginosaconsisting of two core loci responsible for synthesis of PCA and three additional genes encoding unique enzymes involved in the conversion of PCA to pyocyanin, 1-hydroxyphenazine, and phenazine-1-carboxamide.

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Sari, Maulidyanti E., Raihani Wahdah y Bambang Fredricus. "PENGARUH PRIMING DENGAN EKSTRAK TOMAT DAN LAMA PERENDAMAN DENGAN Pseudomonas fluorescens TERHADAP VIABILITAS BENIH TERUNG BORNEO Lu (Solanum melongena L.)". EnviroScienteae 18, n.º 2 (25 de agosto de 2022): 193. http://dx.doi.org/10.20527/es.v18i2.14822.

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The objectives of this study were : To determine the effect of interaction between tomato fruit extract concentration and seeds soaking time with Pseudomonas fluorescens on the viability of eggplant seeds; To determine the effect of each tomato extract concentration and seeds soaking time with Pseudomonas fluorescens on the viability of eggplant seeds; To determine the best combination of tomato fruit extract concentration and seeds soaking time with Pseudomonas fluorescens on the viability of eggplant seeds. This study used a Factorial Completely Randomized Design (CRD) with separate control. The first factor was the concentration of tomato extract (K) and the second factor was soaking time with Pseudomonas fluorescens suspension. Consisting of four levels of tomato extract concentration, three levels of soaking time with of Pseudomonas fluorescens suspension, and one control treatment ((4x3)+1) with three replications each, so that 39 experimental units were obtained. The variables that were observed included seed germination, simultaneous growth, seed growth rate, root length, plumula length, and normal germination dry weight. The results showed that the treatment and control had a very significant effect on the variables of seeds germination and seeds growth rate. The interaction between tomato fruit extract concentration and soaking time with Pseudomonas fluorescens suspension was found in the variable of eggplant seeds germination. The single factor of soaking time with of Pseudomonas fluorescens suspension was found to have an effect on variable seeds growth rate. The best combination on viability of seeds was found in the treatment with 5% tomato extract concentration and the duration of soaking the seeds in Pseudomonas fluorescens suspention for 1 hour.

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Ammeri, Rim Werheni, Ines Mehri, Souhir Badi, Wafa Hassen y Abdenaceur Hassen. "Pentachlorophenol degradation by Pseudomonas fluorescens". Water Quality Research Journal 52, n.º 2 (24 de mayo de 2015): 99–108. http://dx.doi.org/10.2166/wqrj.2017.003.

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Fluorescent Pseudomonads strains were considered as plant growth promoting bacteria. They exhibited antagonistic activities against phytopathogens and showed bio-fertilizing properties. The strain Pseudomonas fluorescens PsWw128, isolated from wastewater, can use the pentachlorophenol (PCP) as the sole source of carbon and energy. High-performance liquid chromatography (HPLC) and spectrophotometric methods were used to follow the PCP degradation and biomass PsWw128 formation. However, the removal efficiency of PCP was highly significant. Thus, PsWw128 was able to degrade more than 99% of PCP when this isolate was grown under a high concentration of PCP (250 mg L–1) in a mineral salts medium (MSM). The simultaneous utilization of glucose and PCP indicates the diauxic growth pattern of PsWw128. PCP addition (100 mg L–1) in the growth medium can contribute to a decrease of the antibiotic susceptibility, and increase the biofilm development. In the presence of the toxic pollutant PCP (100, 200 and 250 mg L–1), the antibiotic sensitivity showed a decrease concerning the seven antibiotics tested. Furthermore, the biofilm formation appeared very low with OD600 = 0.075 in the Brain infusion broth supplemented with 25% of glucose, and developed a significant growth with an OD600 = 1.809 in the MSM supplemented with 250 mg L–1 of PCP.

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Rajeswari, P. y Rupam Kapoor. "Combined Application of Different Species of Trichoderma and Pseudomonas fluorescens on the Cellulolytic Enzymes of Fusarium Oxysporum for the Control of Fusarium wiltDisease in Arachis hypogea. L". Biosciences, Biotechnology Research Asia 14, n.º 3 (25 de septiembre de 2017): 1169–76. http://dx.doi.org/10.13005/bbra/2557.

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ABSTRACT: Fusarium oxysporum causes Fusarium wilt of crop plants leads to considerable yield loss. The study was conducted to determine the beneficial effects of combining Trichoderma species and Pseudomonas fluorescens i.e Trichodema viride+ Pseudomonas fluorescens (Tv+Pf) (1+2%), Trichoderma harzianum+Pseudomonas fluorescens (Th+Pf) (1.5+2%), Trichoderma viride +Trichoderma harzianum (Tv+Th) (1+1.5%) on the activity of cellulolytic enzymes of Fusarium oxysporum to control Fusarium wilt of Arachis hypogaea. L wilt in vitro. The activity of 1,4 -β – Endoglucanase, 1,4 -β – Exoglucanase, Cellobiases produced by Fusarium oxysporum (Control) was higher. Maximum inhibition of Cellulolytic enzymes was shown by culture filtrate of Trichoderma viride + Pseudomonas fluorescens (Tv+Pf) (1+2%), followed by Trichoderma harzianum + Pseudomonas fluorescens, (Th +Pf) (1.5+2%) and Trichoderma viride + Trichoderma harzianum (Tv+Th) (1+1.5%). However, disease suppression of Fusarium wilt of Arachis hypogaea. L by the compatible combination of Trichodema viride + Pseudomonas fluorescens (1+2%) was considerably better as compared to other two strains. At the same time the other two combinations resulted in enhanced disease suppression as compared to single strains. This indicates that the potential benefits of using combination treatments to suppress Fusarium wilt. The study suggests the significance of interactive effects of Trichoderma and Pseudomonas in biocontrol of wilt disease.

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Mohan, Vathsala, Reginald Wibisono, Saili Chalke, Graham Fletcher y Françoise Leroi. "The Anti-Listeria Activity of Pseudomonas fluorescens Isolated from the Horticultural Environment in New Zealand". Pathogens 12, n.º 2 (19 de febrero de 2023): 349. http://dx.doi.org/10.3390/pathogens12020349.

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Beneficial bacteria with antibacterial properties are attractive alternatives to chemical-based antibacterial or bactericidal agents. Our study sourced such bacteria from horticultural produce and environments to explore the mechanisms of their antimicrobial properties. Five strains of Pseudomonas fluorescens were studied that possessed antibacterial activity against the pathogen Listeria monocytogenes. The vegetative culture of these strains (Pseudomonas fluorescens-PFR46I06, Pseudomonas fluorescens-PFR46H06, Pseudomonas fluorescens-PFR46H07, Pseudomonas fluorescens-PFR46H08 and Pseudomonas fluorescens-PFR46H09) were tested against Listeria monocytogenes (n = 31), Listeria seeligeri (n = 1) and Listeria innocua (n = 1) isolated from seafood and horticultural sources and from clinical cases (n = 2) using solid media coculture and liquid media coculture. All Listeria strains were inhibited by all strains of P. fluorescens; however, P. fluorescens-PFR46H07, P. fluorescens-PFR46H08 and P. fluorescens-PFR46H09 on solid media showed good inhibition, with average zones of inhibition of 14.8 mm, 15.1 mm and 18.2 mm, respectively, and the other two strains and P. fluorescens-PFR46H09 had a significantly greater zone of inhibition than the others (p < 0.05). There was no inhibition observed in liquid media coculture or in P. fluorescens culture supernatants against Listeria spp. by any of the P. fluorescens strains. Therefore, we hypothesized that the structural apparatus that causes cell-to-cell contact may play a role in the ejection of ant-listeria molecules on solid media to inhibit Listeria isolates, and we investigated the structural protein differences using whole-cell lysate proteomics. We paid special attention to the type VI secretion system (TSS-T6SS) for the transfer of effector proteins or bacteriocins. We found significant differences in the peptide profiles and protein summaries between these isolates’ lysates, and PFR46H06 and PFR46H07 possessed the fewest secretion system structural proteins (12 and 11, respectively), while PFR46H08 and PFR46H09 had 18 each. P. fluorescens-PFR46H09, which showed the highest antimicrobial effect, had nine tss-T6SS structural proteins compared to only four in the other three strains.

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Tomer, Ajay, Ramji Singh, Durga Prasad y Saurabh Kumar Singh. "INFLUENCE OF SEED BIOPRIMING WITH DIFFERENT ISOLATES OF PSEUDOMONAS FLUORESCENS ON THE GROWTH OF PADDY". Journal of Biopesticides 13, n.º 02 (1 de diciembre de 2020): 103–9. http://dx.doi.org/10.57182/jbiopestic.13.2.103-109.

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ABSTRACT Many strains of the Pseudomonas fluorescens have been characterized as plant growth promoting rhizobacteria (PGPR) and they enhance the growth and yield of economical important paddy crops. The effect of bio-priming on paddy paddy seed growth was assessed at Crop Research Centre S.V.B.P.A.&T Meerut India by studying the effect of fluorescent Pseudomonas strains for their growth stimulatory effect on paddy plants in Randomized Block Design (RBD) in pot conditions. A total of nineteen isolates of the rhizobacteria Pseudomonas fluorescens were selected from the fields of paddy, wheat, mustard, chilli, sorghum and pearlmillet and these strains were used as bio-priming agents for paddy paddy seed. The highest increase in shoot dry weight was seen in the isolate Pseudomonas fluorescens SVP 12 followed by SVP 11 and SVP 13 with 93.33, 86.67 and 80 per cent enhancement in shoot dry weight. All isolates of P. fluorecens, when applied as seed biopriming of paddy paddy seed, had some stimulatory effect on growth and physiological parameters of treated plants indicating that such strains could be use for enhancing the yield and quality of paddy paddy crop.

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Mlipano, Chove Lucy, Grandison Alistair y Lewis Michael. "Detection of Proteolysis in Milk by Pseudomonas fluorescens Using Urea PAGE Method". Journal of Food Studies 7, n.º 1 (27 de diciembre de 2017): 14. http://dx.doi.org/10.5296/jfs.v7i1.12019.

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Proteolysis of milk during storage by two strains of Pseudomonas NCIMB 702085 (414) and NCIMB 701274 (416) was investigated using the Urea PAGE method. Pseudomonas fluorescens enzymes were also extracted and purified by dialysis before inoculation into UHT skim milk in an attempt to partially purify the enzyme. Results showed that dialysis removed some peptides and amino acids which would interfere with the assay procedure. The method also confirmed that Pseudomonas fluorescens NCIMB 701274 (416) was more proteolytic than Pseudomonas NCIMB 702085 (414). Thus, Urea PAGE is a useful method for monitoring proteolysis in milk by Pseudomonas fluorescens.

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Kumar Maurya, Manoj, Ramji Singh y Ajay Tomer. "IN VITRO EVALUATION OF ANTAGONISTIC ACTIVITY OF PSEUDOMONAS FLUORESCENSAGAINST FUNGAL PATHOGEN". Journal of Biopesticides 07, n.º 01 (1 de junio de 2014): 43–46. http://dx.doi.org/10.57182/jbiopestic.7.1.43-46.

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ABSTRACT The present investigation was undertaken to isolate different strains of Pseudomonas fluorescens from various agroecological zones or crop’s rhizosphere like moong, brinjal, rice, chilli, mustard, chirchida and tomato. Totally eight micro flora resembling Pseudomonas fluorescens were isolatedand three isolates were confirmed as P. fluorescens (strain P.f.01, strain P.f.05 and strain P.f.07). Pseudomonas fluorescens strains P.f 07 were found most effective with the highest antagonisticactivity against three fungal pathogen and show maximum inhibition of mycelial growth of Fusariummoniliforme (65.45%), Rhizoctonia solani (68.23%), and Alternaria alternat(48.13%).

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Schmidt, C. S., F. Agostini, C. Leifert, K. Killham y C. E. Mullins. "Influence of Soil Temperature and Matric Potential on Sugar Beet Seedling Colonization and Suppression of Pythium Damping-Off by the Antagonistic Bacteria Pseudomonas fluorescens and Bacillus subtilis". Phytopathology® 94, n.º 4 (abril de 2004): 351–63. http://dx.doi.org/10.1094/phyto.2004.94.4.351.

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Pseudomonas fluorescens B5 and Bacillus subtilis MBI 600 colonized sugar beet seedlings at matric potentials of -7 × 103, -140 × 103, and -330 × 103 Pa and under five temperature regimes ranging from 7 to 35°C, with diurnal fluctuations of 5 to 22°C. No interaction between matric potential and temperature was observed. In situ bioluminescence indicated physiological activity of Pseudomonas fluorescens B5. Colonization of the root at ≥4 cm below the seed decreased at very low matric potential (-330 × 103 Pa). Total population size of Pseudomonas fluorescens B5 per seedling was significantly increased at -140 × 103 Pa. However, matric potential had no significant effect on the population density of Pseudomonas fluorescens per gram of root fresh weight and did not affect the distribution of the population down the root. Total population size per seedling and downward colonization by Pseudomonas fluorescens B5 were significantly reduced at high temperatures (25 to 35°C). Maximum colonization down the root occurred at intermediate temperature (15°C) at both matric potentials (-7 × 103 and -140 × 103 Pa). Addition of B. subtilis MBI 600 to the seed had no effect on rhizosphere populations of Pseudomonas fluorescens B5. Populations of B. subtilis MBI 600, which consisted largely of spores, were slightly reduced at lower matric potentials and were not affected by temperature. Survival and dry weight of plants in soils infested with Pythium spp. decreased with increasing soil temperature and matric potential, indicating an increase in disease pressure. However, there was no significant interaction between the two factors. At -330 × 103 Pa, soil dryness but not Pythium infection was the limiting factor for plant emergence. At temperatures of 7 to 25°C and matric potentials of -7 × 103 to 120 × 103 Pa, treatment with Pseudomonas fluorescens B5 increased plant survival and dry weight. At 7°C and -120 × 103 Pa, there was almost complete emergence of seeds treated with Pseudomonas fluorescens B5. Antagonistic activity of Pseudomonas fluorescens B5 decreased with increasing soil temperature and decreasing matric potential. At 25 to 35°C and -7 × 103 Pa, no effect was observed. In regimes with different day and night temperatures, the maximum (day) temperature was decisive for disease development and antagonistic activity. B. subtilis MBI 600 displayed no significant antagonistic effect against Pythium ultimum and did not influence the performance of Pseudomonas fluorescens B5 in combined inocula.

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Shweta, R. K. S. Tiwari y Vinod Kumar Nirmalkar. "Efficacy of various bio-control agents for the management of leaf spot of turmeric (Curcuma longa L.) caused by Taphrina maculans". INTERNATIONAL JOURNAL OF PLANT SCIENCES 17, n.º 1 (15 de enero de 2022): 32–36. http://dx.doi.org/10.15740/has/ijps/17.1/32-36.

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A field experiment was conducted in the last week of June 2020, at Horticulture Research cum Instructional Farm of Barrister Thakur Chhedilal College of Agriculture and Research Station, Sarkanda, Bilaspur (C.G.), to test the efficacy of various bio-control agents for the management of leaf spot of turmeric caused by Taphrina maculans. Treatment include the bio-agents alone or different combinations viz. Trichoderma harzianum, Pseudomonas fluorescens, Bacillus subtilis, Trichoderma harzianum + Pseudomonas fluorescens, Trichoderma harzianum + Bacillus subtilis, Pseudomonas fluorescens + Bacillus subtilis and Trichoderma harzianum + Pseudomonas+ Bacillus subtilis and chemical fungicide mancozeb for foliar spray at 35 days after appearance of disease. Foliar spray of combination Pseudomonas fluorescens at 45 days after appearance of disease significantly reduced percent diseases index of taphrina leaf spot (PDI 43.57 %) and enhanced fresh rhizome yield (20.85 t ha-1) compared to other bio-control agents applications.

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ASAI, Hisae, Hiromichi ONOZAKI y Hidemasa IMASEKI. "Vanillylamine metabolism in Pseudomonas fluorescens." Agricultural and Biological Chemistry 52, n.º 11 (1988): 2741–46. http://dx.doi.org/10.1271/bbb1961.52.2741.

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SIMOR, A. E., J. RICCI, A. LAU, R. M. BANNATYNE y L. FORD-JONES. "Pseudobacteremia due to Pseudomonas fluorescens". Pediatric Infectious Disease Journal 4, n.º 5 (septiembre de 1985): 508–12. http://dx.doi.org/10.1097/00006454-198509000-00014.

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Collignon, P., D. Dreimanis y W. Beckingham. "Pseudobacteraemia due to Pseudomonas fluorescens". Journal of Hospital Infection 43, n.º 4 (diciembre de 1999): 321–22. http://dx.doi.org/10.1016/s0195-6701(99)90434-6.

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Anderson, Shawna y Vasu D. Appanna. "Indium detoxification in Pseudomonas fluorescens". Environmental Pollution 82, n.º 1 (1993): 33–37. http://dx.doi.org/10.1016/0269-7491(93)90159-l.

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KURESHI, AMINA y HARY SUSEELAN. "CAVITARY PNEUMONIA FROM PSEUDOMONAS FLUORESCENS". CHEST 164, n.º 4 (octubre de 2023): A1441. http://dx.doi.org/10.1016/j.chest.2023.07.1000.

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Sutariati, G. A. K., T. C. Rakian, A. Madiki, N. M. Rahni, G. N. A. Wibawa y L. Mudi. "Effectiveness of a single and a mixture treatments of rhizobacteria in increasing the growth and yield of hot pepper (Capsicum annuum L.)". IOP Conference Series: Earth and Environmental Science 977, n.º 1 (1 de junio de 2022): 012044. http://dx.doi.org/10.1088/1755-1315/977/1/012044.

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Abstract Concern for the safety and public health of the environmental impact of the use of chemical pesticides has prompted more consideration of the use of environmentally friendly plant cultivation technologies as a natural approach to improve plant health. This study aimed to evaluate the effectiveness of seed treatment with rhizobacteria Bacillus sp.CKD061, Pseudomonas sp. SWRIIB04 and Pseudomonas fluorescens PG01 alone or in mixture on the growth and yield of hot pepper plants. The study was arranged using a randomized block design consisting of 8 rhizobacteria treatments, namely control, Bacillus sp.CKD061 (C), Pseudomonas sp. SWRIIB04 (S), P. fluorescens PG01 (P), mixture of Bacillus sp.CKD061 and Pseudomonas sp. SWRIIB04 (C+S), mixture of Bacillus sp.CKD061 and P. fluorescens PG01 (C+P), mixture of Pseudomonas sp. SWRIIB04 and P. fluorescens PG01 (S+P), mixture of Bacillus sp.CKD061, Pseudomonas sp. SWRIIB04 and P. fluorescens PG01 (C+S+P). The data obtained were analyzed using analysis of variance, followed by Duncan’s Multiple Range Test. The results showed that the seed treatment using a mixture of rhizobacteria Bacillus sp.CKD061 and Pseudomonas sp. SWRIIB04 was the most effective treatment in increasing the growth and yield of hot pepper plants. The increase in hot pepper production reached 90% compared to the control.

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Behrendt, Undine, Andreas Ulrich, Peter Schumann, Jean-Marie Meyer y Cathrin Spröer. "Pseudomonas lurida sp. nov., a fluorescent species associated with the phyllosphere of grasses". International Journal of Systematic and Evolutionary Microbiology 57, n.º 5 (1 de mayo de 2007): 979–85. http://dx.doi.org/10.1099/ijs.0.64793-0.

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The taxonomic position of a group of fluorescent pseudomonad strains isolated from the phyllosphere of grasses was investigated through a polyphasic approach. Riboprinting analysis revealed highly similar patterns for the investigated strains which supported, together with the agreement of many phenotypic characteristics, their affiliation to the same species. A comparison of 16S rRNA gene sequences of strain P 513/18T, a representative strain from the grass isolates, revealed that it was affiliated to the cluster of the ‘Pseudomonas fluorescens group’, with Pseudomonas costantinii as the closest phylogenetic neighbour. However, DNA–DNA hybridization showed a clear demarcation at the species level between strain P 513/18T and P. costantinii. Furthermore, a comparison of riboprint patterns with Pseudomonas species clustering next to the novel grass isolates on the basis of 16S rRNA gene sequences supported their separate species status at the phylogenetic level. Based on phenotypic features, the novel isolates could also be differentiated from the other fluorescent Pseudomonas species that share positive arginine dihydrolase and oxidase reactions. As a consequence of these phenotypic and phylogenetic analyses, the isolates from the grass pyllosphere represent a novel species for which the name Pseudomonas lurida sp. nov. is proposed. The type strain is P 513/18T (=DSM 15835T=LMG 21995T).

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Budzikiewicz, H., S. Kilz, K. Taraz y J. M. Meyer. "Identical Pyoverdines from Pseudomonas fluorescens 9AW and from Pseudomonas putida 9BW". Zeitschrift für Naturforschung C 52, n.º 11-12 (1 de diciembre de 1997): 721–28. http://dx.doi.org/10.1515/znc-1997-11-1202.

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Abstract Pseudomonas fluorescens, Pseudomonas putida, Pyoverdine, Siderophore, Bacterial Classification From Pseudom onas fluorescens 9AW and from Pseudomonas putida 9BW identical pyo-verdine-type siderophores were isolated and their structures were elucidated by spectroscopic methods and degradation studies. These novel compounds are of interest as they contain L-threo-β-hydroxy histidine in their peptide chains, an amino acid sofar encountered in nature only rarely. The co-occurence of the same pyoverdine in different Pseudom onas species and its significance for the classification is discussed.

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Watson, Tristan T., Tom A. Forge y Louise M. Nelson. "Pseudomonads contribute to regulation ofPratylenchus penetrans(Nematoda) populations on apple". Canadian Journal of Microbiology 64, n.º 11 (noviembre de 2018): 775–85. http://dx.doi.org/10.1139/cjm-2018-0040.

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Inoculation with antagonistic soil microorganisms has shown potential to suppress replant disease of apple in orchard soils. Pseudomonas spp. may have the potential to reduce Pratylenchus penetrans populations on apple. Pseudomonas spp. were isolated from the rhizosphere of sweet cherry and screened for antagonistic characteristics. Two highly antagonistic Pseudomonas isolates, P10-32 and P10-42, were evaluated for growth promotion of apple seedlings, suppression of P. penetrans populations, and root colonization in soil from three orchards. During the isolate screening, Pseudomonas fluorescens P10-32 reduced in vitro growth of fungal pathogens, had protease activity, had capacity to produce pyrrolnitrin, suppressed P. penetrans populations, and increased plant biomass. Pseudomonas fluorescens P10-42 reduced in vitro growth of fungal pathogens, had protease activity, suppressed P. penetrans populations, and increased plant biomass. In potted orchard soil, inoculating apple with P. fluorescens P10-32 suppressed P. penetrans populations in one of the three soils examined. Inoculation with P. fluorescens P10-42 improved plant growth in two of the soils and suppressed P. penetrans abundance in one soil. In one of the soils, P. fluorescens P10-42 was detected on the roots 56 days postinoculation. Overall, we conclude that Pseudomonas spp. play a role in suppressing P. penetrans on apple in orchard soil.

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Saidi, Aluna Utilma, Mintarto Martosudiro y Abdul Latief Abadi. "Induction Resistance in Chili (Capsicum frutescens L.) to the Geminivirus Disease by Pseudomonas fluorescens". Research Journal of Life Science 9, n.º 2 (1 de agosto de 2022): 47–60. http://dx.doi.org/10.21776/ub.rjls.2022.009.02.1.

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This study included resistance induced by Pseudomonas fluorescens PGPR in chili (Capsicum frutescens L.) infected with Geminivirus. Geminivirus often attacks chili plants, causing disease with a yellowish color to the leaves, lobes, and stunting. Chili plants that have been attacked by the virus will experience crop failure and plant death. Virus prevention can be done with the application of PGPR by Pseudomonas fluorescens. The method used was 6 treatments on chili plants which were repeated 4 times. The analysis of variance was processed using R software. The results showed that cayenne pepper plants that were given the PGPR application had a shorter incubation period against Geminivirus attacks. Each concentration of Pseudomonas fluorescens has a mean incubation period that is not significantly different. The results also showed that there was a significant difference in the intensity of the disease that attacked the cayenne pepper plant. The application of 107 Pseudomonas fluorescens showed the most optimal results for phenol content, plant height, and number of plant leaves. The application of 109 Pseudomonas fluorescens showed optimal results for the content of IAA and the content of the peroxidase enzyme.

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Nielsen, T. H., D. Sørensen, C. Tobiasen, J. B. Andersen, C. Christophersen, M. Givskov y J. Sørensen. "Antibiotic and Biosurfactant Properties of Cyclic Lipopeptides Produced by Fluorescent Pseudomonas spp. from the Sugar Beet Rhizosphere". Applied and Environmental Microbiology 68, n.º 7 (julio de 2002): 3416–23. http://dx.doi.org/10.1128/aem.68.7.3416-3423.2002.

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ABSTRACT Cyclic lipopeptides (CLPs) with antibiotic and biosurfactant properties are produced by a number of soil bacteria, including fluorescent Pseudomonas spp. To provide new and efficient strains for the biological control of root-pathogenic fungi in agricultural crops, we isolated approximately 600 fluorescent Pseudomonas spp. from two different agricultural soils by using three different growth media. CLP production was observed in a large proportion of the strains (approximately 60%) inhabiting the sandy soil, compared to a low proportion (approximately 6%) in the loamy soil. Chemical structure analysis revealed that all CLPs could be clustered into two major groups, each consisting of four subgroups. The two major groups varied primarily in the number of amino acids in the cyclic peptide moiety, while each of the subgroups could be differentiated by substitutions of specific amino acids in the peptide moiety. Production of specific CLPs could be affiliated with Pseudomonas fluorescens strain groups belonging to biotype I, V, or VI. In vitro analysis using both purified CLPs and whole-cell P. fluorescens preparations demonstrated that all CLPs exhibited strong biosurfactant properties and that some also had antibiotic properties towards root-pathogenic microfungi. The CLP-producing P. fluorescens strains provide a useful resource for selection of biological control agents, whether a single strain or a consortium of strains was used to maximize the synergistic effect of multiple antagonistic traits in the inoculum.

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Roshani, Zaya, Srinivasaraghavan A., Kalmesh Managanvi, Erayya y Ramesh Nath Gupta. "Characterization of Florescent Pseudomonads for Biological control Efficacy, Plant Growth Promotion and Antibiotic Tolerance". International Journal of Environment and Climate Change 13, n.º 10 (28 de agosto de 2023): 1456–66. http://dx.doi.org/10.9734/ijecc/2023/v13i102800.

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A collection of fourteen indigenous Fluorescent Pseudomonads (FLPs) was isolated from diverse crop rhizospheres across different regions of Bihar, and their distinctive characteristics were documented. The differentiation between Pseudomonas aeruginosa and Pseudomonas fluorescens was achieved through pigmentation, fluorescence assessment on King's A media, and growth analysis at 42°C. Among the isolates, nine were identified as P. aeruginosa: FLP strains associated with Rice, Turmeric, Mustard-1, Mustard-2, Okra-G, Pea, Barley, Brinjal, and Chickpea. Meanwhile, five isolates - FLP Wheat, FLP Bean, FLP Mango, FLP Brinjal New, and FLP Cauliflower - were classified as P. fluorescens. Confirmation of these classifications was established through DNA sequencing of the 16s RNA region. These FLP isolates were evaluated against prevalent phytopathogens, including Macrophomina phaseolina, Colletotrichum musae, Fusarium oxysporum f. sp. cubense, and Xanthomonas oryzae pv. oryzae. Notable performance was exhibited by FLP Barley (70.55%) against Macrophomina phaseolina, FLP Okra-G (53.82%) against Colletotrichum musae, FLP Rice (38.58%) against Fusarium oxysporum f. sp. cubense, and FLP Okra-G against Xanthomonas oryzae pv. oryzae. In the context of biocontrol efficacy, FLP Turmeric, FLP Pea, FLP Okra-G, and FLP Mustard-1 showcased superior performance in combatting phytopathogens. Additionally, all FLP isolates, except FLP Cauliflower, exhibited phosphate-solubilizing capabilities, with FLP Brinjal demonstrating the highest solubilization (Phosphate Solubilizing Index of 2.89). Siderophore production was a common trait among the isolates. Compatibility with antibiotics, namely Streptocycline at concentrations of 0.025% and 0.10%, as well as Copper oxychloride at 0.25%, 0.30%, and 0.50%, was assessed. Only FLP Cauliflower exhibited compatibility with both antibiotics across all concentrations. Notably, FLP Rice, FLP Turmeric, and FLP Barley demonstrated positive antibiotic compatibility alongside effective biocontrol potential. In summation, this study unveils the distinct attributes and potential applications of indigenous Fluorescent Pseudomonads (FLPs) isolated from the rhizospheres of diverse crops in Bihar. The identified isolates exhibit promising traits such as disease control, phosphate solubilization, siderophore production, and antibiotic compatibility, thus presenting valuable options for sustainable agricultural practices and disease management.

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Circella, Elena, Antonella Schiavone, Roberta Barrasso, Antonio Camarda, Nicola Pugliese y Giancarlo Bozzo. "Pseudomonas azotoformans Belonging to Pseudomonas fluorescens Group as Causative Agent of Blue Coloration in Carcasses of Slaughterhouse Rabbits". Animals 10, n.º 2 (6 de febrero de 2020): 256. http://dx.doi.org/10.3390/ani10020256.

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The study describes the finding of an abnormal blue-tinged color found on rabbit carcasses in the refrigeration cell of two butcher shops in Apulia Region. The carcasses were from an industrial rabbitry for production of meat with a regularly authorized slaughterhouse. Pseudomonas azotoformans, a microorganism included in Pseudomonas fluorescens group, was isolated from samples collected by the altered carcasses, showing the growth of uniform bacterial colonies with fluorescent pigmentation. The bacterium was also isolated from an additional water sample and from the labelling gun collected in the slaughterhouse, whilst the knives used for slaughtering resulted negative. Chromatic alteration was experimentally reproduced on new carcasses using a 108 cfu/mL bacterial suspension prepared with the isolated strain. Due to their resistance characteristics, members of P. fluorescens group are very difficult to eradicate once introduced into the production environment. Therefore, their presence, even if not considered a public health problem, should be monitored by food industry operators in self-control plans.

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48

Wuryantoro, Wuryantoro, Wuye Ria Andayanie y Ndaru Hadian Dhuhava. "Penggunaan Agens Hayati Pseudomonas fluorescens terhadap Pertumbuhan Tanaman Kedelai (Glycine max L. Merr,)". JURNAL AGRI-TEK : Jurnal Penelitian Ilmu-Ilmu Eksakta 22, n.º 2 (23 de diciembre de 2021): 78–81. http://dx.doi.org/10.33319/agtek.v22i2.100.

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Abstractâ€”Soybean (Glycine max L.) is the third most important food commodity after rice and corn in Indonesia. This study aims to determine the interaction of soybean growth that has been incubated using Pseudomonas fluorescens. The research method used a randomized block design experiment consisting of five treatments, namely S1 (soybean seeds were given Pseudomonas fluorescens and not incubated), S2 (soybean seeds were given Pseudomonas flourescen and incubated for 6 hours), S3 (soybean seeds were given Pseudomonas flourescens and incubated for 12 hours), S4 (soybean seeds were given Pseudomonas flourescens and incubated for 18 hours), and S5 as a control. The test used univariate analysis and further tested with Duncan's test with a level of 5%. The results showed that there was no significant effect on the use of Pseudomonas flourescens as a soybean seed incubation material on the parameters of plant height, leaf area, wet weight, and dry weight of soybean plants.Â Keywordsâ€”:Soybean; Pseudomonas fluorescens; incubation; seeds; biological agents

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Dhiman, Shivali, Santosh Kumari, Anuj Sohi y Balbir S. Dogra. "Residual Soil Fertility and Yield of Okra as Affected by Bio-inoculants and Bio-organic Nutrient Sources". International Journal of Plant & Soil Science 36, n.º 6 (26 de abril de 2024): 156–64. http://dx.doi.org/10.9734/ijpss/2024/v36i64617.

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Over the course of two kharif seasons in 2020 and 2021, a study was conducted to explore the impact of bi-inoculants (Pseudomonas fluorescens and Pseudomonas lactis) and bio-organic nutrient sources (farmyard manure, vermicompost, Beejamrit and Jeevamrit) over the fruit yield and residual soil fertility of okra in the Entisols of Himachal Pradesh. The study was structured around seventeen treatments with different combinations of bio-inoculants, bio-formulations and nutrient sources. The investigation revealed that minimum soil pH (6.96), maximum soil organic carbon (0.76 %), available nitrogen (259.36 kg/ha) and available phosphorus (26.98 kg/ha) were obtained with treatment T16 [FYM (50 q/ha) + Vermicompost (25 q/ha) + Jeevamrit + Pseudomonas fluorescens]. The maximum soil electrical conductivity (0.208 dSm-1) and available potassium (174.65 kg/ha) was obtained in treatment T17 [Recommended Dose of Fertilizer (78N:50P:54K kg/ha)]. The highest gross income (₹2,58,760 /ha), net income (₹1,60,620 /ha) were observed in treatment T16 [FYM (50 q/ha) + Vermicompost (25q/ha) + Jeevamrit + Pseudomonas fluorescens] and the highest benefit: cost ratio (1.69) was observed in treatment T10 [Jeevamrit + Pseudomonas fluorescens]. Therefore, it is evident that using bio-organic nutrients such as farmyard manure, vermicompost, and Jeevamrit, in conjunction with the bacterium Pseudomonas fluorescens, is advantageous for enhancing the residual fertility of the soil and achieving sustainable cultivation of okra, while also allowing for a complete savings of 100% on fertilizers.

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50

Yu, Chang Li, Zhi Peng Lu, Fa Zhi Ge y Er Li Zhao. "Biosorption of Cadmium onto Pseudomonas fluorescens: Application of Isotherm and Kinetic Models". Advanced Materials Research 171-172 (diciembre de 2010): 49–52. http://dx.doi.org/10.4028/www.scientific.net/amr.171-172.49.

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The present study was undertaken to evaluate the feasibility of Pseudomonas fluorescens biomass for the removal of cadmium ions from aqueous solutions. Batch experiments were performed to study the adsorption of cadmium on pH, Pseudomonas fluorescens biomass adsorbent with respect to initial Cd(II) concentration, contact time and biomass dose. The experimental data were modeled by Langmuir and Freundlich isotherm models. Langmuir model resulted in the best fit of the adsorption data. The maximum adsorption capacity for Cd(II) was 66.25 mg/g (pH 5.0 and 5 g/L biomass dose). Kinetics of adsorption followed second-order rate equations. The FTIR results of Pseudomonas fluorescens biomass showed that biomass has different functional groups and these functional groups are able to react with metal ion in aqueous solution. The results of the present study suggest that Pseudomonas fluorescens biomass can be used beneficially in treating industrial effluents containing heavy metal ions.

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