Tesis sobre el tema "Neuronal differentiation"
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Rocha, Joana Fernandes da. "Understanding APP-dependent neuronal differentiation". Master's thesis, Universidade de Aveiro, 2011. http://hdl.handle.net/10773/7389.
Texto completoAmyloid Precursor Protein (APP) is a type 1 membrane protein that suffers proteolytic cleavages and has been implicated in roles such as cell adherence, survival, migration and differentiation. Although a role in neuritogenesis has been attributed to APP, some contradictory results have been reported regarding the benefits of knocking-down or overexpressing APP. Further, while the addition of the APP proteolytic sAPP (secreted APP) fragment to the cell medium enhances neuritogenesis, the amount of cellular APP and other APP fragments may be deleterious for this process. Further, preliminary work from the Neuroscience laboratory of the Center for Cell Biology indicated that pAPP (APP phosphorylated at the S655 residue) can potentially be crucial in APPmediated neuronal differentiation, for example by increasing APP cleavage to its biological fragment sAPP or APP binding to specific signal transducers. In this work, the capacity of APP and pAPP to mediate neuronal differentiation was tested, in the initial period of retinoic acid (RA)-induced SH-SY5Y cells differentiation. These neuroblastoma cells are a well documented neuronal-like cell model used in neuronal differentiation studies. Several molecular tools were used, including wild-type and phosphomutants APP-GFP. The evaluation of differentiation included neuritogenic output analysis by bright field and epifluorescence microscopy, using various approaches. Namely scoring the number of differentiated cells and performing morphometric analyses of transfected cells and of the all cellular population. The levels of APP and medium secreted sAPP, and of cytoskeleton-related proteins and posttranslational modifications, such as MAP2, Acetylated Tubulin and Actin were also quantified by Western blot analysis, and related to the morphological parameters. Additionally, the potential role of AICD in APP-mediated neuronal differentiation was inferred from pharmacologic assays, where its generation is inhibited. Together the results obtained show that APP, sAPP and AICD modulate neuritogenesis in a complex and well-ordered manner. While long-term increases in APP can be detrimental to neuronal-like differentiation, in an AICD-dependent manner, short-term increases benefit this process in an APP S655 phosphorylation dependent manner, potentially involving sAPP secretion and specific cytoskeleton rearrangements.
A Proteína Precursora de Amilóide de Alzheimer (PPA) é uma proteína membranar tipo 1 sujeita a processamento proteolítico que tem sido associada a funções como adesão celular, sobrevivência, migração e diferenciação. Apesar de lhe terem sido atribuídas funções na neuritogénese, os dados experimentais obtidos até à data que envolveram modulação dos níveis da PPA revelam-se contraditórios. De facto, enquanto a adição do fragmento PPA secretado (PPAs) ao meio celular favorece a neuritogénese, a quantidade de PPA celular e de outros fragmentos da PPA poderão já não constituir um benefício para este processo. Adicionalmente, dados preliminares do laboratório de Neurociências do Centro de Biologia Celular sugerem que a PPAp (PPA fosforilada na S655) poderá ser fundamental na diferenciação neuronal mediada pela PPA, nomeadamente por aumentar a proteólise da PPA a PPAs ou a ligação da PPA a sinais de transdução específicos. No presente trabalho, avaliou-se a capacidade da PPA e PPAp em mediar o período inicial de diferenciação neuronal induzida por ácido retinóico. Para tal recorreu-se a células de neuroblastoma SH-SY5Y, um modelo celular do tipo neuronal bem estabelecido para estudos de diferenciação. Adicionalmente, várias ferramentas moleculares, como PPA-GFP selvagem e fosfomutantes foram usadas. A avaliação da diferenciação incluiu a análise de vários parâmetros neuritogénicos por microscopia de luz (de campo claro e de fluorescência), nomeadamente monitorização de células diferenciadas e análises morfométricas das células transfectadas e da população geral. Os níveis de PPA e PPAs, e de proteínas relacionadas com citosqueleto e suas modificações pós-traducionais (MAP2, Tubulina Acetilada e Actina) também foram quantificados. Além do mais, a influência do DIP na diferenciação neuronal dependente de PPA foi avaliada usando um composto farmacológico para inibir a sua produção. De um modo geral, os resultados obtidos demonstram que a PPA, PPAs e DIP modulam a neuritogénese de um modo complexo e ordenado. Enquanto a indução de níveis altos de expressão de PPA (48h) podem ser detrimentais para a diferenciação tipo-neuronal, de uma forma dependente de DIP, induções mais breves (24h) beneficiam este processo de um modo dependente da fosforilação na S655, potencialmente envolvendo a secreção de PPA e rearranjos específicos do citosqueleto.
Unsworth, Harriet Christina. "Connexins in neuronal and epidermal differentiation". Thesis, Queen Mary, University of London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.429065.
Texto completoGore, S. "Neuronal differentiation markers in basal cell carcinoma". Thesis, University College London (University of London), 2007. http://discovery.ucl.ac.uk/1445574/.
Texto completoHigginbotham, Holden Richard. "Polarity regulation during neuronal migration and differentiation". Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p3315121.
Texto completoTitle from first page of PDF file (viewed Aug. 4, 2008). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 150-172).
CECI, CLAUDIA. "Effect of nickel exposure on neuronal differentiation". Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2013. http://hdl.handle.net/2108/203180.
Texto completoHein, Paul. "A role for C/EBP[beta] in neuronal differentiation and neuronal regeneration /". Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100623.
Texto completoCortical progenitor cell fate involves collaborations between cell-intrinsic factors and extracellular cue-activated signalling pathways. Similarly, the activity of C/EBP family members is regulated by cell-intrinsic factors and by the extrinsically activated Erk 1/2-C/EBP signalling pathway in several differentiating non-neuronal cells. Here we present evidence of an analogous function for C/EBP family members in neurogenesis. The results presented in this thesis show that (1) inhibition of MEK (upstream of Erk 1/2) or inhibition of C/EBP family members blocks cortical progenitor neurogenesis; (2) inhibition of C/EBP family members promotes CNTF-induced astrogenesis; and (3) forced expression of a C/EBPbeta mutant (that is, a mimic of its Erk 1/2-RSK phosphorylated form) enhances the expression of neuron-specific genes such as Talpha1 alpha-tubulin.
Neuronal regeneration involves the re-activation of some development-associated genes such as Talpha1 alpha-tubulin and GAP-43. To further define the role of C/EBP family members in the transcription of the Talpha1 alpha-tubulin gene in neurons during regeneration, we crossed transgenic mice that express the beta-galactosidase gene under the control of the Talpha1 alpha-tubulin minimal promoter (Talpha1MP:nLacZ) with mice that carry a null mutation for either C/EBPbeta or C/EBPdelta. The results of facial nerve crush experiments conducted on these hybrid mice show that C/EBPbeta plays a role in the transcriptional activation of the Talpha1 alpha-tubulin minimal promoter following neuronal injury. Injury-induced mRNA expression for either Talpha1 alpha-tubulin or GAP-43 was not noticeably affected by the absence of C/EBPbeta. This suggests that C/EBPbeta-independent mechanisms also play a role in neuronal regeneration.
Lochter, André. "Control of neuronal differentiation by extracellular matrix constituents /". [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10325.
Texto completoDe, las Heras Rachel y n/a. "Neuronal Differentiation: A Study Into Differential Gene Expression". Griffith University. School of Biomolecular and Biomedical Science, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040225.161725.
Texto completoMarote, Ana Maria Franco Aveiro. "The effects of piezoelectric polymers on neuronal differentiation". Master's thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/11630.
Texto completoO crescimento de neurites é crucial para o desenvolvimento neuronal, bem como para a plasticidade e reparação na fase adulta. Após uma lesão neuronal, o sucesso da reparação é determinando pelas propriedades plásticas constitutivas dos neurónios afetados e pelo seu potencial de regeneração, que é influenciado por sinais externos físicos (ex.: cicatriz glial) e químicos (ex.: moléculas inibitórias). Recentemente, o desenvolvimento de materiais à nano-escala, que interagem com os sistemas biológicos a nível molecular, prometem revolucionar o tratamento das lesões do Sistema Nervoso Central e Periférico. Os scaffolds de nanomateriais podem suportar e promover o crescimento de neurites e consequentemente, intervir nas complexas interações moleculares que ocorrem a após o dano neuronal, entre as células e o seu ambiente extracelular. Vários estudos têm demonstrado que os materiais piezoeléctricos, que geram carga elétrica em resposta ao stress mecânico, podem ser usados para a preparação de scaffolds eletricamente carregados que devem influenciar o comportamento celular. Este estudo centrou-se nos efeitos dos materiais baseados em PLLA (ácido poli (L – láctico)) sob a forma de filmes, nanofibras orientadas aleatória e alinhadamente, e da sua polarização, na diferenciação neuronal. A linha celular de neuroblastoma (SH-SY5Y) foi utilizada para avaliar o efeito dos materiais-baseados em PLLA na adesão, viabilidade, morfologia celular, bem como na diferenciação tipo-neuronal. A análise proteómica baseada em espectrometria de massa das células cultivadas em nanofibras de PLLA foi também efetuada. Os neurónios corticais embriónicos foram seguidamente utilizados para avaliar os efeitos das nanofibras de PLLA alinhadas e da sua polarização no crescimento de neurites. Nesta análise, descobrimos que os materiais de PLLA parecem inibir parcialmente a proliferação celular, enquanto promovem a diferenciação, alterando os níveis das proteínas que intervêm nestes processos. Ocorrem alterações significativas do citoesqueleto, particularmente ao nível do citoesqueleto de actina, que não induzem mas parecem potenciar o crescimento de neurites sob exposição a um sinal extracelular como o ácido retinóico. Este efeito parece ser particularmente evidente para as nanofibras de PLLA alinhadas, que induzem efeitos intermédios na restruturação do citoesqueleto. Em geral, a polarização das amostras de PLLA tem efeitos benéficos na proliferação celular e potencia o crescimento de neurites, particularmente nos neurónios. Acreditamos que as nanofibras de PLLA alinhadas serão um bom scaffold para regeneração neuronal, uma vez que mimetiza o ambiente mecânico natural das células. Contudo, futuras experiências in vitro e in vivo são necessárias para comprovar a eficácia deste potencial scaffold.
Neuritic growth is crucial for neural development, as well as for adaptation and repair in adulthood. Upon neuronal injury, the successful neuritic regrowth is determined by the constitutive plastic properties of neurons and by their regenerative potential, which is influenced by physical (e.g. glial scar) and chemical (e.g. inhibitory molecules) extrinsic cues. Recently, the development of nanometer-scale materials, which can interact with biological systems at a molecular level, provide hope to revolutionize the treatment of central and peripheral nervous system injuries. Nanomaterial scaffolds can support and promote neuritic outgrowth and consequently, take part in the complex molecular interactions between cells and their extracellular environment after neuronal injury. Several studies have shown that piezoelectric materials, which generate electrical charge in response to mechanical strain, may be used to prepare bioactive electrically charged scaffolds that may influence cell behavior. This study focused on the effects of PLLA (poly-L-lactic acid) – based materials in the form of films, random and aligned nanofibers, and of their polarization, on neuronal-like and neuronal differentiation. The neuroblastoma SH-SY5Y cell line was used to evaluate the effect of PLLA – based materials on cellular adhesion, viability, morphology and neuron-like differentiation. Mass spectrometry-based proteomic analysis of cells grown on PLLA nanofibers was also conducted. Primary embryonic cortical neurons were further used to evaluate the effect of PLLA aligned nanofibers and their polarization on neuritic outgrowth. In this analysis, we found that PLLA materials seem to partially inhibit cell proliferation, while promoting neuronal differentiation, altering the levels of proteins that intervene in these processes. Dramatic cytoskeleton remodeling occurs, particularly at the actin cytoskeleton level, which does not induce but may potentiate neuritic outgrowth upon exposure to an extracellular cue, such as Retinoic Acid. This effect seems to be particularly evident for PLLA aligned nanofibers, which induce intermediate effects in the cytoskeleton remodeling. In general, polarization of the PLLA polymers has beneficial effects on cell proliferation and potentiates the neuritic outgrowth, particularly in neurons. We believe that polarized PLLA aligned nanofibers would be a good scaffold for neuronal regeneration, since it mimics the natural mechanical cell environment and enhances neuritic outgrowth. However, further in vitro and in vivo investigations are required to prove the efficacy of this potential scaffold.
De, las Heras Rachel. "Neuronal Differentiation: A Study Into Differential Gene Expression". Thesis, Griffith University, 2003. http://hdl.handle.net/10072/367735.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Sciences
Full Text
Hill, Donna Monique. "Mechanism of centaurin-alpha-1 control of neuronal differentiation". Birmingham, Ala. : University of Alabama at Birmingham, 2010. https://www.mhsl.uab.edu/dt/2010m/hill.pdf.
Texto completoTitle from PDF t.p. (viewed June 30, 2010). Additional advisors: Lori McMahon, Stephen Watts. Includes bibliographical references (p. 31-35).
Lortie, Karine. "The growth-arrest-specific protein gas7 potentiates neuronal differentiation". Thesis, University of Ottawa (Canada), 2004. http://hdl.handle.net/10393/26701.
Texto completoHarrison, Alexander W. J. "Ionotropic and metabotropic signalling in neuronal development and differentiation". Thesis, Cardiff University, 2012. http://orca.cf.ac.uk/17785/.
Texto completoScott, Sarah Anne. "The effect of glial signalling on dopamine neuronal differentiation". Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612827.
Texto completoDutan, Polit Lucia Margarita. "Identifying gene regulatory networks during neuronal differentiation of iPSCs". Thesis, King's College London (University of London), 2018. https://kclpure.kcl.ac.uk/portal/en/theses/identifying-gene-regulatory-networks-during-neuronal-differentiation-of-ipscs(624a488f-ff78-4da0-982c-169943681ddd).html.
Texto completoRuhe, Larissa. "Investigation of cap-independent translation initiation in neuronal differentiation". Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/21184.
Texto completoTranslation initiation is a complex and highly regulated process which involves the assembly of an elongation competent ribosome on the mRNA. The vast majority of eukaryotic mRNAs is translated by a canonical cap-dependent mechanism. This requires the eIF4F protein complex to bind the mRNA at the 5’-cap to recruit further eIFs and the small ribosomal subunit which then scans the 5’UTR in 5’ to 3’ direction until a start codon is encountered. Afterwards the large ribosomal subunit joins and protein synthesis begins. Besides that, translation of mRNAs can be mediated by IRESs, internal ribosome entry sites, which recruit the ribosome in a cap and 5’-end-independent manner to the start codon. Such cellular IRES-mediated translation is thought to be inefficient under physiological conditions but activated during stress. As the regulation of this mechanism is not well understood, we aimed to elucidate cellular cap-independent translation events. Therefore, we generated a mouse embryonic stem cell line with inducible overexpression of a dominant negative mutant of 4E-BP1. 4E-BP1 sequesters the cap-binding protein eIF4E so that the eIF4F protein complex fails to assemble at the 5’-cap. We performed shotgun proteomics during 4E‑BP1 overexpression and neuronal differentiation to globally monitor translation dynamics. Genes with reduced sensitivity for cap-dependent translation were identified and tested for internal translation initiation in bicistronic reporter assays. After stringent validation one cap-independently translated mRNA, Pqbp1, was discovered. The second part of this study investigated cap-independent translation initiation on a circRNA, which by nature lacks free ends and thus requires IRES-mediated translation. We could show that circMbl is translated in vitro and thus contributed to the scientific evidence for the translation of circRNAs in fly brain, which was studied in a collaboration project.
Lewis, Philip Alexander. "The role of N-Src kinases in neuronal differentiation". Thesis, University of York, 2014. http://etheses.whiterose.ac.uk/8000/.
Texto completoNishimura, Rocky Hiroki. "The Adenosine Receptor and Serum Deprivation-Induced Neuronal Differentiation". Thesis, Griffith University, 2004. http://hdl.handle.net/10072/365767.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Science
Full Text
Doszyn, Olga. "Sex differences in neuronal differentiation of human stem cells". Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-384661.
Texto completoChoi, Olivia J. "Spag17 Deficiency Impairs Neuronal Cell Differentiation in Developing Brain". VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/5877.
Texto completoSalani, Monica. "Acetylcholine modulation of neuronal differentiation: involvement of transcriptional factors". Doctoral thesis, La Sapienza, 2006. http://hdl.handle.net/11573/916859.
Texto completoFATO, PAMELA. "EVALUATION OF THE GM1 OLIGOSACCHARIDE ROLE IN NEURONAL DIFFERENTIATION". Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/796885.
Texto completoGriffin, Síle Marie. "Directing stem cell differentiation towards a neuronal fate using nicotinamide". Thesis, Keele University, 2016. http://eprints.keele.ac.uk/2379/.
Texto completoBani-Yaghoub, Mahmud. "The role of gap junctions in neuronal and astroglial differentiation". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0013/NQ42495.pdf.
Texto completoMarçal, Nathalie. "Promotion of cortical neuronal differentiation by groucho-related gene 6". Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82288.
Texto completoTonge, Peter D. "Monitoring neuronal differentiation in the embryonal carcinoma cell line NTERA2". Thesis, University of Sheffield, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.487636.
Texto completoMak, Wing-yan Grace y 麥詠恩. "Functional characterization of CDK5RAP3 in hepatocellular carcinoma and neuronal differentiation". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45151982.
Texto completoChatzi, Christina. "Derivation, maintenance and neuronal differentiation of mouse embryonic stem cells". Thesis, University of Aberdeen, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.446228.
Texto completoBrown, Matthew David. "Collapsin response mediator protein and Rho GTPases in neuronal differentiation". Thesis, University College London (University of London), 2004. http://discovery.ucl.ac.uk/1446689/.
Texto completoMark, Melanie Danelle. "The mechanisms underlying EGF-stimulated neuronal differentiation in PC12 cells /". Thesis, Connect to this title online; UW restricted, 1996. http://hdl.handle.net/1773/6261.
Texto completoPoon, Hoi-fung. "Characterization of the roles of PAK5 in neuronal cell differentiation". Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43085933.
Texto completoYu, Chung Ho. "Effects of melia toosendan on neuronal differentiation of PC12 cells /". View Abstract or Full-Text, 2002. http://library.ust.hk/cgi/db/thesis.pl?BICH%202002%20YU.
Texto completoIncludes bibliographical references (leaves 137-165). Also available in electronic version. Access restricted to campus users.
BERSAN, Emanuela. "Characterization of new stem cell niches with neuronal differentiation potential". Doctoral thesis, Università degli Studi di Verona, 2010. http://hdl.handle.net/11562/341480.
Texto completoAdult neural stem cells (NSC), have been found in the main neurogenic regions of brain, i.e. hippocampus, sub ventricular zone (SVZ), olfactory bulb, and in some non-neurogenic regions, i.e. spinal cord. Other brain sites could host NSC niches and, in particular, considering the role of meninges in correct cortex development we were interested in exploring the region residing between arachnoide and the first layers of the cerebral cortex, called Leptomeninges. Aim of this project is characterized the leptomeningeal compartment as potential niche for neural stem cells with ex vivo and in vitro approaches. The leptomeningeal compartment has been characterized by immunohistochemistry at different rat ages, from embryo E20, postnatal day 0 (P0), P15 and adult. We found a(nestin) neuro-epithelial stem cells marker positive cells layer with decreasing thickness from embryo up to adult. Nestin positive cells were distributed outside the basal lamina (marked by laminin), and as a distinct population from astrocytes (stained with GFAP) and oligodendrocytes (stained with NG2). Nestin positive cells were dissected and expanded in vitro from P0, P15 and adult rats leptomeninges. We were able to culture them as homogeneus nestin positive cells population in adherent condition In neuronal differentiating conditions, nestin positive cells mainly differentiate into MAP2 positive cells but also GFAP and O4 (marker for mature oligodendrocyte) positive cells were detected in culture. As a first level of functional evaluation of differentiated cells, their ability to depolarize has been analyzed by calcium imaging assay after Fura-2 loading. In vitro differentiated neurones responded to fast applications of the depolarizing agent KCl suggesting the expression of voltage dependent calcium channels, similar to that of functional neurons. As following step, the in vivo neuronal differentiation potential was assessed by infusion of expanded EGFP LeSC in rat hippocampus. Engrafted LeSC were monitored by immunofluorescence up two months and during this period LeSC were able to survive after injection. About half of EGFP cells engrafted in hippocampus, expressed neuronal markers (DCX, MAP2, NeuN, Neurofilament-160, GAD67) and shown differentiated neuronal morphology. Because of the persistence of these cells up to adulthood, their proliferation capability in vitro, and their differentiation potential into neuronal cells in vitro and in vivo, we suggest to name them leptomeningeal stem/progenitor cells (LeSC) as a new population never described before. Since meninges cover whole brain, also Leptomeninges from rat spinal cord has been analyzed. Nestin positive cells were distributed as previously observed in the brain, outside the basal lamina, and as a distinct population from astrocytes and oligodendrocytes. Cells were dissected and kept in culture as neurosphere and resulted positive for nestin, MAP2, GFAP, O4, and Oct4. A new study In collaboration with professor M. Schwartz group (Weizmann Institute, Rehovot, Israel) is ongoing to understand the potential role of immune system in regulating leptomeninges and LeSC (as suggested by previous publications from Schwartz’s group). Preliminary results Comparison of LeSC proliferation and nestin expression by immunohistochemistry in SCID vs wt mice, revealed a significant decrease of nestin positive LeSC in SCID mice. However total cell number and proliferating cells in leptomeninges were not changed. Further characterizations are ongoing to understand the phenotype of proliferating nestin negative cells in meninges. The importance of Leptomeningeal stem cells reside in the easier reachable localization compared to the already known neural stem cell niches, and in their high neuronal differentiation potential. These characteristics will open novel studies in regenerative medicine.
Poon, Hoi-fung y 潘海鋒. "Characterization of the roles of PAK5 in neuronal celldifferentiation". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B43085933.
Texto completoKnipp, Sabine. "Neuronal migration and differentiation in the developing locust enteric nervous system". kostenfrei, 2009. http://d-nb.info/998023310/34.
Texto completoZhao, Xiaosu. "NRC-interacting factor 1 interacts with p35 and regulates neuronal differentiation /". View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BICH%202006%20ZHAO.
Texto completoCastellanos, Kotkoff Monica Cecilia. "Dimorphic differentiation of female-specific neuronal populations and behavior in Drosophila". Thesis, University of British Columbia, 2014. http://hdl.handle.net/2429/50821.
Texto completoPatel, Radhika. "Investigating the role of alternative polyadenylation in neuronal differentiation and neurodegeneration". Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:17b8b820-b3f4-416a-a8e6-a2915ddf5374.
Texto completoThwaites, J. W. "Methods affecting neuronal differentiation of human adult and pluripotent stem cells". Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1467088/.
Texto completoHolback, Sofia. "Proteolytic processing of the Alzheimer APP protein family during neuronal differentiation". Doctoral thesis, Stockholm : Department of Neurochemistry, Stockholm University, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-31301.
Texto completoTeo, Jia-Ling. "Presenilin-1 and TCF/[beta]-catenin signaling : effects on neuronal differentiation /". Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/9311.
Texto completoHelwig, Bryan Glen. "Neuronal differentiation of stem cells derived from human umbilical cord matrix /". Search for this dissertation online, 2003. http://wwwlib.umi.com/cr/ksu/main.
Texto completoEminel, Sevgi [Verfasser]. "Functions of JNK stresskinases in neuronal apoptosis and differentiation / Sevgi Eminel". Kiel : Universitätsbibliothek Kiel, 2008. http://d-nb.info/1019542004/34.
Texto completoANGHILERI, Elena. "Adipose-derived mesenchymal stem cells: neuronal differentiation potential and neuroprotective action". Doctoral thesis, Università degli Studi di Verona, 2010. http://hdl.handle.net/11562/343866.
Texto completoAdult mesenchymal stem cells derived from adipose tissue (ASC) offer significant practical advantages over other types of stem cells (SC) for potential clinical applications, since they can be obtained from adult adipose tissue in large amounts, can be easily cultured and expanded with a very low risk for development of malignancies. We investigated in vitro the neuronal differentiation potential of human ASC with a chemical protocol and a prolonged two-step protocol, which included sphere formation and sequential culture in brain-derived neurotrophic factor (BDNF) and retinoic acid (RA). After 30 days, about 57% ASC show morphological, immunocytochemical and electrophysiological evidence of initial neuronal differentiation. In fact, ASC display elongated shape with protrusion of two or three cellular processes, selectively express nestin and neuronal molecules (including GABA-A receptor and tyroxine hydroxilase) in the absence of glial phenotypic markers. Differentiated cells show negative membrane potential (−60 mV), delayed rectifier potassium currents and TTX-sensitive sodium currents, but they are unable to generate action potential. Considering the low efficacy and the not-fully mature neuronal differentiation, we evaluated if ASC display a neuroprotective effect. Using the H2O2-stressed neuroblastoma model in vitro, we show that ASC increase cell availability (compared to fibroblasts) and protect against apoptosis. A possible mechanism involved could be the secretion of BDNF, as reported for human BM-MSC: in this regard, we indeed find high levels of BDNF in ASCcondition medium. In addition to exert neuroprotection, soluble factors secreted by ASC promote neurite outgrowth, an additional mechanism that may favor neuroregeneration. In view of these results and their immunosuppressive action (Constantin et al, 2009), ASC may be a ready source of adult MSC to treat neurodegenerative diseases.
Hattori, Yukako. "Subtype-specific postmitotic transcriptional programs controlling dendrite morphogenesis of Drosophila sensory neuron". 京都大学 (Kyoto University), 2014. http://hdl.handle.net/2433/188831.
Texto completoKyoto University (京都大学)
0048
新制・課程博士
博士(生命科学)
甲第18418号
生博第298号
新制||生||39(附属図書館)
31276
京都大学大学院生命科学研究科統合生命科学専攻
(主査)教授 上村 匡, 教授 西田 栄介, 教授 荒木 崇
学位規則第4条第1項該当
Bai, Shoumei. "Role of DNA methyltransferase 3B in neuronal cell differentation". Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1125702764.
Texto completoTitle from first page of PDF file. Document formatted into pages; contains xviii, 157 p.; also includes graphics (some col.). Includes bibliographical references (p. 125-157). Available online via OhioLINK's ETD Center
Albania, Lara. "Longins and the longin domain: pivotal elements in subcellular trafficking and neuronal differentiation". Doctoral thesis, Università degli studi di Padova, 2012. http://hdl.handle.net/11577/3422908.
Texto completoLe proteine SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptors) sono le più studiate nell’ambito del traffico subcellulare, dato il loro ruolo nella formazione del complesso di fusione delle membrane. In questa famiglia, le VAMP lunghe o longine sono caratterizzate da un dominio N-terminale denominato longin (LD), che ha una funzione sia nella formazione del complesso SNARE, sia nella localizzazione subcellulare delle proteine; le longine trovano inoltre un modello in VAMP7, Sec22b e Ykt6. Il LD adotta una conformazione chiusa che risulta stabile, ma non è ancora chiaro il contributo che tale conformazione e ogni differente dominio proteico portano alla determinazione della localizzazione stessa di tali proteine. VAMP7 umana, codificata dal gene SYBL1 è coinvolta in molteplici pathway subcellulari, compreso il controllo della crescita dei neuriti. Lo splicing alternativo nel locus di SYBL1 produce inoltre due famiglie di isoforme che mantengono un’interessante architettura di domini. Le longine non-SNARE condividono il dominio inibitorio LD, mentre le longine non-LD lo SNARE motif. Date le evidenze preliminari che conferiscono tale funzione inibitoria al costrutto artificiale LD e invece un’attività di promozione della crescita al costrutto non-LD, sembra plausibile che le due sottofamiglie rispecchino questi ruoli contrapposti. Tuttavia, i meccanismi coinvolti nella neuritogenesi non sono ancora completamente chiariti, soprattutto per quanto concerne il contributo degli stimoli extracellulari e delle diverse proteine SNARE. Questo lavoro di tesi si è incentrato sulla caratterizzazione del LD di VAMP7 e delle isoforme di splicing di questa proteina nell’ambito del loro ruolo sia nella localizzazione subcellulare, che nel differenziamento neuronale. Analisi di espressione in diversi tessuti e linee cellulari, dati quantitativi di real time RT-PCR e analisi di microscopia confocale hanno dimostrato come le varianti di VAMP7 presentino differenti tessuto-specificità e localizzazioni subcellulari; l’isoforma VAMP7i mostra inoltre una localizzazione anche nucleare. Considerando la loro diversa combinazione di domini, queste varianti di splicing fisiologiche sono state utilizzzate come strumenti per lo studio dei determinanti di localizzazione. Per di più, frammenti ricombinanti della regione citosolica di VAMP7a hanno confermato che i songoli domini non sono in grado da soli di determinare la localizzaione della proteina e che il cambiamento conformazionale aperto/chiuso non è rilevante per la localizzazione subcellulare, in assenza della regione trasmembrana. Esperimenti di gain-of-function su cellule di neuroblastoma e neuroni primari hanno mostrato l’esistenza di una regolazione della crescita dei neuriti mediata dallo splicing alternativo di VAMP7, con la produzione di isoforme sia inibitorie (VAMP7i) che stimolatorie (VAMP7dh). Tali effetti dipendono anche dal substrato (Poli-D-Lisina o Laminina) in cui i neuroni sono cresciuti e dalla co-espressione con altre isoforme di VAMP7 o con altre SNARE (VAMP2), indicando la presenza di un meccanismo di regolazione fine da parte dello splicing alternativo di VAMP7. Ulteriori investigazioni potranno portare sia alla manipolazione della neuritogenesi per scopi di terapeutici, sia al chiarimento del ruolo che le specifiche varianti di splicing possono avere in alcune malattie neurologiche. La futura caratterizzaione del LD di VAMP7 e delle sue isoforme, ad esempio il ruolo di VAMP7i nel nucleo possono definire nuovi interattori e meccanismi molecolari, utili in alcune applicazioni biotecnologiche.
Gustafsson, Sofia. "Cannabinoids as modulators of cancer cell viability, neuronal differentiation, and embryonal development". Doctoral thesis, Umeå universitet, Farmakologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-51560.
Texto completoMoore, Carlene Drucilla. "The role of centaurin alpha-1 in the regulation of neuronal differentiation". Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2008. https://www.mhsl.uab.edu/dt/2008d/moore.pdf.
Texto completoSartor, Francesca. "Regulation of translation initiation and RNA decay is important for neuronal differentiation". Thesis, University of Aberdeen, 2016. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=.
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