Tesis sobre el tema "Multiplex"
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BARBOSA, Ana Clara de Oliveira Ferraz. "Avaliação de critérios de compatibilidade entre pares de primers para otimização de sistemas multiplex de genotipagem". Universidade Federal de Goiás, 2010. http://repositorio.bc.ufg.br/tede/handle/tde/1291.
Texto completoThe progress of Molecular Biology and Genetics provided the appearance of several molecular markers that detect the genetic polymorphism directly at DNA. Among these markers are the microsatellites (SSR), which are distinguished by their high degree of polymorphism. The use of these markers for individual genotyping has evolved into multiplex systems, which allow many SSR fragments to be detected and analyzed simultaneously. Currently there are several articles in literature discussing the criteria to be used in the primer design for use in PCR, as well as various softwares are available for this end. However, there are few studies and tools for the analysis of compatibility between pairs of primers for use in multiplex systems, where multiple fragments are simultaneously amplified using PCR. This paper evaluated different criteria for compatibility between pairs of primers. A set of 74 combinations of pairs of primers, involving the amplification of 94 SSR loci were evaluated in duplex systems. The same combinations were evaluated according to different criteria, including the degree of complementarity between primers, the magnitude of differences of denaturation temperatures (Tm) and the tendency to annealing between pairs of primers based on the Gibbs free energy resulting from the association between them. The comparison between the different criteria allowed the identification of a set of criteria with positive predictive value equal to 94%. These criteria were implemented for use in a software called Multiplexer, which from the analysis in sequence of pairs of primers, suggests compatible combinations for use in multiplex genotyping systems. Using this tool can significantly reduce the costs related to laboratory activities for genotyping using PCR.
Os avanços da Biologia Molecular e da Genética proporcionaram o surgimento de diversos marcadores moleculares que detectam o polimorfismo genético diretamente no DNA. Entre estes marcadores se encontram os microssatélites (SSR), que se destacam pelo seu elevado grau de polimorfismo. O uso desses marcadores para fins de genotipagem individual tem evoluído para sistemas multiplex, os quais permitem que vários fragmentos SSR sejam detectados e analisados simultaneamente. Atualmente são abundantes na literatura artigos que discutem os critérios a serem utilizados no desenho de pares de primers para aplicação em PCR, bem como estão disponíveis diversos softwares para este fim. No entanto, ainda são escassos os estudos e ferramentas destinados à análise de compatibilidade entre pares de primers para aplicação em sistemas multiplex, onde vários fragmentos são amplificados simultaneamente por PCR. Neste trabalho são avaliados diferentes critérios de compatibilidade entre pares de primers. Um conjunto de 74 combinações de pares de primers, envolvendo a amplificação de 94 locos SSR foram avaliados em sistemas duplex. As mesmas combinações foram avaliadas segundo diferentes critérios, incluindo o grau de complementariedade entre primers, magnitude das diferenças de temperaturas de desnaturação (Tm) e a tendência ao anelamento entre pares de primers com base na energia livre de Gibbs resultante da associação entre eles. A comparação entre os diferentes critérios permitiu a identificação de um conjunto de critérios com valor preditivo positivo igual a 94%. Estes critérios foram implementados para utilização em um software denominado Multiplexer, que a partir da análise de sequências de pares de primers, sugere combinações compatíveis para a utilização em sistemas de genotipagem multiplex. O uso dessa ferramenta pode reduzir consideravelmente os custos laboratoriais relativos às atividades de genotipagem utilizando PCR.
Dahl, Fredrik. "Selector Technology : For Multiplex DNA Analysis". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5921.
Texto completoRen, Haolin. "Visualizing media with interactive multiplex networks". Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0036/document.
Texto completoNowadays, information follows complex paths: information propagation involving on-line editors, 24-hour news providers and social medias following entangled paths acting on information content and perception. This thesis studies the adaptation of classical graph measurements to multiplex graphs, to build visualizations from several graphical representations of the networks, and to combine them (synchronized multi-view visualizations, hybrid representations, etc.). Emphasis is placed on the modes of interaction allowing to take in hand the multiplex nature (multilayer) of the networks. These representations and interactive manipulations are also based on the calculation of indicators specific to multiplex networks. The work is based on two main datasets: one is a 12-year archive of the Japanese public daily broadcast NHK News 7, from 2001 to 2013. Another lists the participants in the French TV/radio shows between 2010 and 2015. Two visualization systems based on a Web interface have been developed for multiplex network analysis, which we call "Visual Cloud" and "Laputa". In the Visual Cloud, we formally define a notion of similarity between concepts and groups of concepts that we call co-occurrence possibility (CP). According to this definition, we propose a hierarchical classification algorithm. We aggregate the layers in a multiplex network of documents, and integrate that hierarchy into an interactive word cloud. Here we improve the traditional word cloud layout algorithms so as to preserve the constraints on the concept hierarchy. The Laputa system is intended for the complex analysis of dense and multidimensional temporal networks. To do this, it associates a graph with a segmentation. The segmentation by communities, by attributes, or by time slices, forms views of this graph. In order to associate these views with the global whole, we use Sankey diagrams to reveal the evolution of the communities (diagrams that we have increased with a semantic zoom). This thesis allows us to browse three aspects of the most interesting aspects of the data miming and BigData applied to multimedia archives: The Volume since our archives are immense and reach orders of magnitude that are usually not practicable for the visualization; Velocity, because of the temporal nature of our data (by definition). The Variety that is a corollary of the richness of multimedia data and of all that one may wish to want to investigate. What we can retain from this thesis is that we met each of these three challenges by taking an answer in the form of a multiplex network analysis. These structures are always at the heart of our work, whether in the criteria for filtering edges using the Simmelian backbone algorithm, or in the superposition of time slices in the complex networks, or much more directly in the combinations of visual and textual semantic indices for which we extract hierarchies allowing our visualization
Wegner, Margret [Verfasser]. "Arthrogryposis multiplex congenita : Eine Klassifikation / Margret Wegner". Aachen : Shaker, 2007. http://d-nb.info/117052771X/34.
Texto completoRios, Villanueva Xavier. "Toward Multiplex Genome Engineering in Mammalian Cells". Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:11179.
Texto completoBattiston, Federico. "The structure and dynamics of multiplex networks". Thesis, Queen Mary, University of London, 2017. http://qmro.qmul.ac.uk/xmlui/handle/123456789/30631.
Texto completoAllen, James. "The public goods game on multiplex networks". Thesis, University of Surrey, 2018. http://epubs.surrey.ac.uk/845834/.
Texto completoVorce, Richard G. "A TUNABLE MULTIPLEX DISCRIMINATOR WITH DIGITAL OUTPUT". International Foundation for Telemetering, 1985. http://hdl.handle.net/10150/615551.
Texto completoThe unit to be described differs from present tunable discriminator products chiefly because it is designed to simultaneously demodulate all the subcarrier channels in a frequency division multiplex. In addition the demodulated output data is presented in digital format that is compatible for direct computer entry. The discriminator implementation techniques will be discussed at the block diagram level. Particular emphasis will be given to the use of “Finite Impulse Response” filters and also to the internal tape speed compensation process.
Qishan, Zhang, Zhang Wenguan y Mu Zhiying. "THE APPLICATION OF ORTHOGONAL FUNCTION TO MULTIPLEX". International Foundation for Telemetering, 1985. http://hdl.handle.net/10150/615706.
Texto completoA unified approach to the multiplex is proposed in terms of the orthogonal functions. It is called quadrature division multiplex, or Q D M in short. The orthogonal function is essential to the multiplex. Except these functions mentioned above, there are other orthogonal functions which are suitable for engineering practice. The orthogonality of the functions is used for the division of signals. A block diagram of Q D M is briefly described. The perfomances of the Q D M system are analysed. The speciality of the Q D M is simple and good. A model of the Q D M is built in our laboratory. A New type of bridge function is presented. It is called copy-shift bridge functions, which may be very useful for multiplex.
Young, Mitchell Patrick. "A 3-D Multiplex Paper-microfluidic Platform". DigitalCommons@CalPoly, 2016. https://digitalcommons.calpoly.edu/theses/1680.
Texto completoNovoa, Del Toro Elva Maria. "Detecting active modules in multiplex biological networks". Thesis, Aix-Marseille, 2020. http://theses.univ-amu.fr.lama.univ-amu.fr/200514_NOVOADELTORO_173hc776k263go601dzf_TH(1).pdf.
Texto completoGene expression is regulated in time, cell types and conditions. We have access to technologies allowing us to measure the gene expression. We can therefore calculate the differences in gene expression between patients and controls, thereby identifying deregulated genes. We can also try to find significant enrichment of one or more cellular functions from the list of deregulated genes. I analyzed transcriptomics data corresponding to Hutchinson-Gilford Progeria Syndrome (HGPS) patients and healthy controls. Our analyses led to the identification of candidate RNAs for experimental validation.Inside cells the molecules do not act isolated, but they interact to accomplish their functions. Nowadays, we have experimental techniques to decipher these interactions on a large scale. Biological interactions can be represented as networks, where the nodes represent molecules, and the edges represent physical and/or functional relationships. The main hypothesis I followed during my thesis is that dense subnetworks associated to an overall expression deregulation correspond to affected cellular processes in patients. I integrated gene expression data and networks to identify such modules. I developed MOGAMUN, a multi-objective genetic algorithm that seeks for active modules. MOGAMUN is the first active module identification algorithm able to consider multiplex networks, i.e. networks composed of different layers of biological interactions
Schaich, Frank. "Wellenlängen-Zeit-Codierung für dichten Wellenlängen-Multiplex /". Aachen : Shaker, 2008. http://d-nb.info/990928616/04.
Texto completoVörös, András. "The emergence of multiple status systems in adolescent communities : a multiplex network theory of group formation". Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:9590194f-84e9-4548-b1fe-cf2f64ffc329.
Texto completoWang, Yu. "Development of a multiplex fluorescent immunoassay for the simultaneous detection of serum antibodies to multiple swine pathogens". Thesis, Kansas State University, 2013. http://hdl.handle.net/2097/15704.
Texto completoDepartment of Diagnostic Medicine and Pathobiology
Raymond R. R. Rowland
Three economically important swine diseases: Porcine Reproductive and Respiratory Syndrome (PRRS), Porcine Circovirus Associated Disease (PCVAD) and Swine influenza cost the US swine industry more than a billion dollars each year. This study developed a fluorescent microsphere immunoassay (FMIA) to simultaneously detect antibodies to the causative pathogens: PRRSV, porcine circovirus (PCV2) and swine influenza virus (SIV). The results showed that the multiplex assay possessed the predicted specificities. In the case of PRRSV NA, the assay displayed higher sensitivity when compared to a commercially available ELISA. The assay was employed to measure both IgG and IgM responses. The FMIA was found to possess several advantages over standard ELISA which include reduced sample volume, time and cost and provides a new tool for veterinary diagnostics. The FMIA was applied for swine disease surveillance in Hawaiian and Texan feral swine populations. The antibodies against PCV2 showed the highest prevalence among these three pathogens in both Hawaii and Texas. Hence we consider PCV2 as the most prevalent pathogen in Hawaiian and Texan feral pigs and this pathogen poses the greatest threat to commercial pigs. SIV seroprevelance increased from 2007 to 2010 in Hawaii State, suggesting an increasing risk for commercial pigs. Moreover, yearly surveillance in Texas State shows growth in seropositive response to all pathogens, particularly PCV2. The development of FMIA for detection of antibodies to multiple swine pathogens in serum samples offers an important alternative for swine disease surveillance in commercial and feral herds.
Kirchner, Piero. "Multiplex-System für digitale Datenströme in multimedialen Anwendungen /". Hamburg : New-Business-Verl, 2010. http://d-nb.info/1000027724/04.
Texto completoStella, Massimo. "Network structure and dynamics of empirical multiplex systems". Thesis, University of Southampton, 2017. https://eprints.soton.ac.uk/419488/.
Texto completoCambronero, Christoffer. "Multiplex protein data and how to handle it". Thesis, Uppsala universitet, Statistiska institutionen, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297390.
Texto completoRamos, Elisabete Manuela de Sousa. "Sistemas Multiplex para a deteção e caracterização molecular de infeções". Master's thesis, [s.n.], 2012. http://hdl.handle.net/10284/3575.
Texto completoAtualmente existe uma enorme preocupação em estudar os microrganismos, em particular os patogénicos para o Homem, uma vez que a sua presença no organismo constitui um risco potencial para a saúde Humana. Por este motivo, nos últimos 10-20 anos, temos assistido a uma evolução significativa nas técnicas disponíveis para a identificação laboratorial dos agentes responsáveis por patologias de base infeciosa. Os novos métodos, especialmente os baseados na tecnologia dos ácidos nucleicos, apresentam enormes vantagens em termos de sensibilidade, especificidade e grande rapidez na obtenção de resultados. Por este motivo, têm sido cada vez mais utilizados na deteção e identificação de microrganismos com interesse diagnóstico, prognóstico e de orientação do tratamento. Diversas tecnologias moleculares são utilizadas em laboratório para detetar agentes patogénicos. As mais utilizadas dependem de plataformas de PCR, e mais recentemente de PCR-em-tempo-real, disponibilizando sensibilidade, especificidade e rapidez sem precedentes na história da microbiologia e virologia clínica. No entanto a elevada especificidade destas técnicas podem também constituir uma desvantagem em situações clínicas que possam ter etiologias associadas a um largo espetro de microrganismos. Por este motivo têm sido desenvolvidas estratégias moleculares para detetar simultaneamente múltiplos agentes: os designados Sistemas Multiplex. O primeiro destes sistemas a ser desenvolvido foi o PCR Multiplex o qual permite detetar e amplificar simultaneamente mais do que um organismo identificando assim o agente etiológico. Mais recentemente têm sido desenvolvidos outros métodos multiplex com o mesmo fim: Técnicas baseadas em Microchips (de hibridização ou de amplificação as quais permitem a deteção e identificação de até milhares de sequências e consequentemente centenas a milhares de agentes patogénicos em simultâneo) bem comos sistemas baseados na tecnologia X-Map (fusão da citometria de fluxo e hibridização em solução com partículas fluorescentes). Do mesmo modo, os sistemas baseados na amplificação e sequenciação do gene rRNA 16S permitem a deteção e identificação de praticamente qualquer bactéria patogénica, sem necessitar de qualquer suspeita prévia. Finalmente, o advento das técnicas de sequenciação de 2ª e 3ª geração permitiu o desenvolvimento de uma nova área científica: a Metagenómica, a qual procura caracterizar simultaneamente e completamente a composição microbiológica de ecossistemas complexos, sejam eles humanos (pele, urina, fezes, boca, etc) ou ambientais (ar, águas residuais, solo, etc). Atualmente existe uma enorme preocupação em estudar os microrganismos, em particular os patogénicos para o Homem, uma vez que a sua presença no organismo constitui um risco potencial para a saúde Humana. Por este motivo, nos últimos 10-20 anos, temos assistido a uma evolução significativa nas técnicas disponíveis para a identificação laboratorial dos agentes responsáveis por patologias de base infeciosa. Os novos métodos, especialmente os baseados na tecnologia dos ácidos nucleicos, apresentam enormes vantagens em termos de sensibilidade, especificidade e grande rapidez na obtenção de resultados. Por este motivo, têm sido cada vez mais utilizados na deteção e identificação de microrganismos com interesse diagnóstico, prognóstico e de orientação do tratamento. Diversas tecnologias moleculares são utilizadas em laboratório para detetar agentes patogénicos. As mais utilizadas dependem de plataformas de PCR, e mais recentemente de PCR-em-tempo-real, disponibilizando sensibilidade, especificidade e rapidez sem precedentes na história da microbiologia e virologia clínica. No entanto a elevada especificidade destas técnicas podem também constituir uma desvantagem em situações clínicas que possam ter etiologias associadas a um largo espetro de microrganismos. Por este motivo têm sido desenvolvidas estratégias moleculares para detetar simultaneamente múltiplos agentes: os designados Sistemas Multiplex. O primeiro destes sistemas a ser desenvolvido foi o PCR Multiplex o qual permite detetar e amplificar simultaneamente mais do que um organismo identificando assim o agente etiológico. Mais recentemente têm sido desenvolvidos outros métodos multiplex com o mesmo fim: Técnicas baseadas em Microchips (de hibridização ou de amplificação as quais permitem a deteção e identificação de até milhares de sequências e consequentemente centenas a milhares de agentes patogénicos em simultâneo) bem comos sistemas baseados na tecnologia X-Map (fusão da citometria de fluxo e hibridização em solução com partículas fluorescentes). Do mesmo modo, os sistemas baseados na amplificação e sequenciação do gene rRNA 16S permitem a deteção e identificação de praticamente qualquer bactéria patogénica, sem necessitar de qualquer suspeita prévia. Finalmente, o advento das técnicas de sequenciação de 2ª e 3ª geração permitiu o desenvolvimento de uma nova área científica: a Metagenómica, a qual procura caracterizar simultaneamente e completamente a composição microbiológica de ecossistemas complexos, sejam eles humanos (pele, urina, fezes, boca, etc) ou ambientais (ar, águas residuais, solo, etc).
Albuquerque, Renata Chaves. "Avaliação de um ensaio utilizando-se MULTIPLEX-PCR para a detecção de meningites por diferentes agentes bacterianos". Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-24012017-102958/.
Texto completoIn this work, a MULTIPLEX-PCR has conducted for the bacterial DNA detection of S. agalactiae, S.pneumoniae, N. meningitidis, H. influenza and other possible etiologic bacterial meningitis agents. This test combines five different primers that detect simultaneously the crtA gene of N. meningitides, the p6 gene of H. influenza, the fbsA gene of S. agalactiae, the lytA gene of S. pneumoniae and the 16S rDNA universal gene to identify the presence of bacterial agent. From the 447 samples of CSF that were analyzed, the test detected 27 positive samples for bacterial culture and 13 samples with the result of negative culture. These negative culture samples presented biochemical changes, hematological, immunological or microbiological (bacterioscopy) suggestive of meningitis, these data helped in the analysis of the MULTIPLEX-PCR results. This test showed no nonspecific reactions with fungi, viruses and other bacterial agents tested (only amplifying the gene 16S rDNA). The MULTIPLEX-PCR test is a fast, reliable, easy to implement and easily implementable for of bacterial meningitis confirmation. And this method can aid in the meningitis diagnosis with negative culture of CSF, particularly for patients who previously started antibiotic therapy and in the differential diagnosis of bacterial or viral meningitis.
Migoyan, Ara-Shant. "Development and evaluation of procedures and methods for Proseek Multiplex". Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-221287.
Texto completoMir, de Simón Bernat. "Multiplex optical detection system for prosthetic joint infection diagnosis". Doctoral thesis, Universitat Autònoma de Barcelona, 2016. http://hdl.handle.net/10803/400573.
Texto completoProsthetic Joint infection (PJI) is a disease of difficult therapeutic management that causes significant physical and psychological morbidity on patients and is associated with substantial financial burden on the healthcare system. Its incidence oscillates between 0.8-2.4% depending on the affected joint such as wrist, shoulder, elbow, hip and knee. In spite of the relatively low incidence of PJI, the financial burden remains enormous (i.e., $566 million in 2009 alone to the American health care system). Appropriate recognition and management are critical to preserving or restoring adequate function and preventing morbidity. However, late chronic infections are challenging to predict and typically caused by microorganisms that grow in biofilms. In that state, microbes are protected from antimicrobial agents and host immune responses. Therefore, a complex and time-consuming combination of procedures is required for its diagnosis. They include clinical findings, laboratory results from peripheral blood and synovial fluid, microbiological data, histological evaluation of periprosthetic tissue, intraoperative inspection, and, in some cases, radiographic results. Nowadays, microbial culture remains the most widespread technique for identifying the infectious agent, but unfortunately it requires 4-14 days to provide a conclusive diagnosis and has low sensitivity, affecting the prognosis of the infection. In order to develop a fast, accurate, and inexpensive method to improve the diagnosis of PJI, a device based on antibody/aptamer-modified plasmonic nanoparticles and Surface Enhanced Raman Scattering (SERS) spectroscopy was developed in this work. This device for infection diagnosis recognized, with sensitivity down to the single colony-forming unit, the presence of bacterial pathogens in biological fluids. The concept was confirmed with four bacterial agents (S. aureus, E. coli, S. agalactiae and P. aeruginosa). For each targeted pathogen receptor, high SERS efficient nanoparticles with a unique combination of a Raman label and an antibody/aptamer were prepared. The nanoparticles produced a relatively weak Raman signal when they are dispersed in a fluid. In contrast, the presence of one of the targeted pathogens triggered the accumulation of its partner nanoparticles on the antigen-carrying membrane of the microorganism, rapidly reaching full random coverage. Multiple gaps between nanoparticles are then formed that act as optical hotspots in which Raman scattering is enhanced by several orders of magnitude (103) relative to the same number of non-interacting nanoparticles. The resulting surface-enhanced Raman scattering signal is sufficiently intense as to record pathogen-specific inelastic light spectra from the nanoparticle-covered bacteria by driving the sample through a millifluidic channel. The method screened milliliters of different biological fluids (i.e., serum, blood, urine and pleural and ascites fluids) at a flow rate close to 0.1 mL/min. Although the concept was only confirmed with four bacterial agents, the detection method can be expanded to simultaneously identify more pathogens by using the large number of available spectral codes and selective antibodies/aptamers. On the other hand, this device is not yet capable of discerning directly in between resistant and non-resistant microorganisms, but it offers an accurate diagnosis in very short time (less than 15 minutes) as compared with all other currently available methods (i.e., cell culturing, matrix-assisted laser desorption/ionization-time-of-flight, polymerase chain reaction and enzyme-linked immunosorbent assay), and it can monitor the effect of the treatment on the time evolution of the infection.
Granell, Martorell Clara. "From community structure to the physics of multiplex networks". Doctoral thesis, Universitat Rovira i Virgili, 2016. http://hdl.handle.net/10803/365314.
Texto completoUstunel, Eser Kwon Hyuck M. "Time division duplex-wideband code division multiplex (TDD-WCDMA)". Diss., Click here for available full-text of this thesis, 2006. http://library.wichita.edu/digitallibrary/etd/2006/t029.pdf.
Texto completo"May 2006." Title from PDF title page (viewed on October 19, 2006). Thesis adviser: Hyuck M. Kwon. Includes bibliographic references (leaves 40-42).
Mewis, Guido. "Entwicklung einer Multiplex-PCR zum Nachweis Enteropathogener Escherichia coli". [S.l.] : [s.n.], 2004. http://www.diss.fu-berlin.de/2004/93/index.html.
Texto completoRenoust, Benjamin. "Analysis and Visualisation of Edge Entanglement in Multiplex Networks". Phd thesis, Université Sciences et Technologies - Bordeaux I, 2013. http://tel.archives-ouvertes.fr/tel-00942358.
Texto completoKownarumit, Supaporn. "Multiplex screening using enzyme inhibition, fluorescence detection and chemometrics". Thesis, Loughborough University, 2006. https://dspace.lboro.ac.uk/2134/12308.
Texto completoFinski, Alexei. "Multiplex Analytical Measurements in Single Cells of Solid Tissues". Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10652.
Texto completoSchaich, Frank [Verfasser]. "Wellenlängen-Zeit-Codierung für dichten Wellenlängen-Multiplex / Frank Schaich". Aachen : Shaker, 2008. http://d-nb.info/1161305734/34.
Texto completoHaidar, Ibrahim. "Digital code division multiplex techniques for data transmission applications". Thesis, Aston University, 1985. http://publications.aston.ac.uk/8052/.
Texto completoWay, Jaw-Shiow Chu. "Specific detection of Salmonella by multiplex polymerase chain reaction". Diss., The University of Arizona, 1993. http://hdl.handle.net/10150/186207.
Texto completoSoar, Filho Ercy José. "Varius multiplex multiformis : epistemologia do self no pós-modernismo". reponame:Repositório Institucional da UFSC, 1997. https://repositorio.ufsc.br/handle/123456789/112200.
Texto completoChauvat, Anne. "Analyse de réponses lymphocytaires T par Elispot et Fluorospot au cours du suivi de protocoles de vaccination : mise en évidence de l’effet du thiomersal présent dans la préparation vaccinale". Thesis, Paris Est, 2012. http://www.theses.fr/2012PEST0064.
Texto completoThe detection of cellular response via its different T cell subpopulations was proved to be crucial to better understand mechanisms of pathologies and monitor protocols of immunotherapy. Unicellular cytokine measurement not only allows the assessment of their inherent role on immunological response, but also the detection of T lymphocytes (LT) secretion profile which gives information on subpopulations involved, their activation state and their functionality. Detection of the cytokine secreting cells frequency is achievable with Elispot. This test is widely used in clinical trials to monitor vaccine response to diseases such as influenza. Although seroprotection is the recognized parameter to assess anti-influenza vaccine efficiency, cellular response has been proved to play an important role in infection control. Our results highlighted that the use of the whole vaccine to monitor T cell response may induce bias in cytokine detection. Indeed, their excipients can be considered as contaminants, like Thimerosal which is used as a preservative in some vaccines. We demonstrated that it induced detection of small spots in interferon (IFN) Elispot. We proved that these spots were associated with in vitro early abortive T cell activation. However, their size enabled us to remove these unspecific spots from bona fide spots.Fluorospot test is suited for multiplex cytokine analysis. Thus it is more adapted than Elispot to detect cytokine secretion profiles and polyfunctional T cells frequency. It is worth noting that polyfunctionnal Tcells are associated with a better clinical outcome in several pathologies. Our results led to technical validation of this test for detection of two cytokines couples, IFN/interleukin (IL)-10 and IFN/IL-2. Fluorospot showed a better sensitivity than intracellular staining cytometry (ICS) in detection of IFN and IL-10 produced by a cell line transfected with the cDNA encoding these cytokines. Moreover, Fluorospot demonstrated a similar sensitivity than Elispot when used to monitor the immunological response to an anti-influenza vaccine. Furthermore, using this technique, we showed that anti-influenza T cell producing IL-2 were the dominant population of T cells. Isolated IFN producing T cells were less sensitive than the detection of IL-2 to detect specific T cell response against influenza. Therefore our results provided a full validation of Fluorospot test, for the technical part as well as for the clinical part. These conclusions open perspectives of using this method to monitor protocols in a near future
Hélard, Jean-François. "Modulations codées en treillis associées à un multiplex de porteuses orthogonales en présence de canaux affectés de trajets multiples". Rennes 1, 1992. http://www.theses.fr/1992REN1S117.
Texto completoŠifta, Radim. "Kvalita služeb v optických přístupových sítích". Doctoral thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2015. http://www.nusl.cz/ntk/nusl-233668.
Texto completoKibalo, Tom y Ben Miles. "DEFINITION OF A FULLY COMPLIANT IRIG RECORDING SYSTEM FOR TELEMETRY". International Foundation for Telemetering, 1997. http://hdl.handle.net/10150/609774.
Texto completoAlliant Techsystems’ Advanced Technology Applications organization incorporates the latest IRIG standards for range equipment and operation. Over the past five years, our objective has been to assure interoperability among diverse data recording users while achieving technical excellence for our ADARIO(Analog Digital Adaptable Input Output) family of products. In this paper, we summarize 25 years of ADARIO development; technical challenges, risks and processes; as well as our five-year effort to modify and develop our recording system products to meet the evolutionary standards of technical excellence.
Almeida, Isa Azevedo de. "Padronização da tecnica para identificação do Nanoplex Y-STR em amostras de sangue humano". [s.n.], 2003. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290746.
Texto completoDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: Nas últimas décadas, as Ciências Forenses vêm evoluindo de maneira a esclarecer e auxiliar cada vez mais precisamente a justiça. O presente trabalho, através da utilização de 25 amostras de sangue de cadáveres do Instituto Médico Legal de Cuiabá-MT, teve como objetivos: estabelecer a forma de coleta do material biológico através da utilização de cartões FTA@; aprimorar a extração do DNA em amostras de sangue, utilizando a Resina Quelante CHELEX@; aprimorar a PCR utilizando os primers para os loei DYS 3851/11, DYS 3891/11, DYS 390, DYS 391, DYS 392, DYS 393, DYS 19 através da padronização do Nanoplex; comprovar a importância do estudo do cromossomo Y nos casos de investigação de paternidade e em casos forenses. Com o desenvolvimento do trabalho, pôde se observar que os métodos de coleta das amostras de sangue e extração do DNA foram satisfatórios, pois obteve-se uma quantidade de DNA suficiente para o seu estudo. O estudo do Nanoplex fez-se com sucesso, pois, por meio de sua análise em um seqüenciador automático (ASI PRISM DNA Sequencer 377), pôde se detectar os fragmentos, estipulando-se, assim, seus respectivos alelos
Abstract: In the last decades, the Forensic Sciences come evolving in way to clarify and to assist the justice. The present work through the use of 25 samples of corpse blood of the Legal Medical Institute of Cuiabá-MT had as objective: to establish the fonn of collection of the biological material through the use of cards FT A@; to improve the extraction of the DNA in samples of blood being used the Resin Quelante CHELEX@; to improve the PCR using primers for locus DYS 385 1/11, DYS 389 1/11, DYS 390, DYS 391, DYS 392, DYS 393, DYS 19 through the standardization of the Nanoplex; to prove the importance of Y chromosome study in the cases of patemity and in forensic cases. Wlth the development of the work it could be observed that the methods of collection of blood samples and extration of the DNA had been satisfactory, therefore got a enough amount of DNA for its study. The study of the Nanoplex one became successfully, therefore through its analysis in an automatic sequencer (ASI PRISM DNA Sequencer 377), it could be detected the fragments, stipulating its respective alleles
Mestrado
Odontologia Legal e Deontologia
Mestre em Odontologia Legal e Deontologia
Del, Valle Mendoza Juana y Universidad Peruana de Ciencias Aplicadas (UPC). "Diagnóstico de 14 virus respiratorios y 3 gérmenes atípicos en pacientes inmunodeprimidos mediante la técnica RT-PCR multiplex". Universidad Peruana de Ciencias Aplicadas (UPC), 2015. http://hdl.handle.net/10757/345682.
Texto completoClass, Volker. "Entwicklung und Struktur des deutschen Kinomarktes". [S.l. : s.n.], 2003. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB11675626.
Texto completoZandegiacomo, Cella Alice. "Multiplex network analysis with application to biological high-throughput data". Master's thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amslaurea.unibo.it/10495/.
Texto completoSantos, Sílvia Regina dos. "Determinação de sorotipos capsulares de Streptococcus pneumoniae por Multiplex-PCR sequencial". Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-12092012-100134/.
Texto completoS.pneumoniae colonizes the nasopharynx and is a major agent of otitis media, pneumonia, bacteremia and meningitis with high morbidity and mortality. It is estimated that 1.6 million people die of pneumococcal disease every year, mostly children under five years old, mainly in developing countries. The antiphagocytic polissarídica capsule is the main virulence factor of this organism and determine the 93 serotypes known for being the target of pneumococcal vaccines. In the present study was standardized molecular typing by Multiplex PCR molecular typing, which comprises 30 primer pairs grouped into six sequential reactions. We performed antimicrobial susceptibility profile, according to the CLSI 2011 and the most frequent serotype was made by molecular genotyping techniques Multilocus Sequence Typing (MLST) and pulsed-field gel Eletrophoresis (PFGE). We studied 270 pneumococcal strains isolated from 2005 to September 2011, from CSF (13%), blood (76%) and pleural fluid (11%) of 232 patients attended at University Hospital of USP. Typing by Multiplex PCR detected 24 serotypes / serogroups different, which were: 14 (22%), 5 (12%), 12F / A (11%), 6A/B/C (10%), 7F / A (5 %), 1 (4%), 3 (4%), 10A (4%), 19A (4%), 18 A / B / C / F (3%), 4 (3%), 8 (3% ), 23F (3%), 19F (3%) and others (9%) (9V / A, 9N / L, 15A, 22F, 11A / D, 31, 38, 34, 16F, 17F and nontypable). This method showed 100% specificity and 98% sensitivity for the determination of 66% for serogroups and serotypes. Serotypes significantly more common in children under two years were: 14, 6B, 5 and 19F among adults serotypes 5 e12F were predominant. The sensitivity profile in non-meningeal infections was 99% sensitivity and 1% penicillin intermediate resistance to ceftriaxone. For meningeal infections the results showed 73% sensitivity and 27% resistance to penicillin and 88% sensitivity and 12% intermediate resistance to ceftriaxone. Resistance to beta-lactams is linked mainly to serotype 14 was the serotype most isolated, and of these 52 strains were performed MLST and PFGE. MLST found in 51 strains belonging to clone Spain9V-3 (ST 156) which is prevalent in south and southeastern Brazil and a strain with a type of sequence is not deposited. The technique of PFGE found three clusters and four non-related samples, cluster A predominated with 41 (79%) strains with 81.7% similarity between them. Multiplex PCR technique proved to be an excellent tool for the detection of serotypes/serogroups of S. pneumoniae. We did not detect full resistance to penicillin and ceftriaxone in non-meningeal infections showing the importance of use of penicillin in the treatment of pneumococcal non-meningeal disease. There was great genetic similarity among strains of S. pneumoniae serotype 14.
Dannered, Peter y Eric Åkerhielm. "Utlokaslisering till Indien : En fallstudie av Multiplex utlokalisering av ekonomitjänster". Thesis, Uppsala universitet, Företagsekonomiska institutionen, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-169646.
Texto completoSundequist, Blomdahl Kristofer. "An evaluation of random-walk based clustering of multiplex networks". Thesis, Uppsala universitet, Institutionen för informationsteknologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-333027.
Texto completoStenberg, Johan. "Software Tools for Design of Reagents for Multiplex Genetic Analyses". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6832.
Texto completoEreku, Luck Tosan. "Design of microfluidic multiplex cartridge for point of care diagnostics". Thesis, Brunel University, 2017. http://bura.brunel.ac.uk/handle/2438/15331.
Texto completoWanfang, Zhang. "THE TIME DIVISION MULTIPLEX MEASURING SYSTEM FOR SINGLE-TRANSIENT SIGNALS". International Foundation for Telemetering, 1993. http://hdl.handle.net/10150/608868.
Texto completoIn order to reduce the measuring channels for the single-transient signals, the author propose the time division multiplex technique and introduce the method of SAW delay line in this paper. That used method of SAW tap-delay line in this system is different from previous methods consists in making traditional method, which is one-path signal input different delayed multi- path signals output, alter new method, which is simultaneous multi-path signal inputs that are respectively delayed and one-path signal serial output.
Armbruster, Katrin. "Entwicklung einer Multiplex-TaqMan-PCR zur Diagnostik der viralen Enzephalitis". [S.l. : s.n.], 2006. http://nbn-resolving.de/urn:nbn:de:bsz:25-opus-49607.
Texto completoGorgannezhad, Lena. "Advanced Technologies in Rapid and Multiplex Detection of Nucleic acid". Thesis, Griffith University, 2020. http://hdl.handle.net/10072/397045.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environment and Sc
Science, Environment, Engineering and Technology
Full Text
Babair, Yasser Hassan Salih. "Multiplex PCR for diagnosis of herpes viruses in neurological infections". Thesis, University of Manchester, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.488092.
Texto completoCosta, Thadeu Estevam Moreira Maramaldo. "Detecção de transgênicos em alimentos utilizando a técnica Multiplex-PCR". reponame:Repositório Institucional da FIOCRUZ, 2008. https://www.arca.fiocruz.br/handle/icict/9250.
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Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde
Durante o período de 1996 a 2006, a proporção da área global de lavouras biotecnológicas plantadas por países em desenvolvimento aumentou consistentemente a cada ano. Nos últimos dez anos, mais de 40 países adotaram políticas de rotulagem para alimentos geneticamente modificados, mas as características das regulamentações e seu grau de execução variam enormemente. Os efeitos observados dessas políticas sobre a escolha dos consumidores, a informação para os consumidores, comércio dos alimentos, comércio internacional também variam significativamente. No Brasil, o Decreto n. 4.680/03 regulamenta o direito à informação, assegurado pela Lei n. 8.078/90, quanto aos alimentos e ingredientes alimentares destinados ao consumo humano ou animal que contenham ou sejam produzidos a partir de organismos geneticamente modificados (OGMs). O cumprimento da legislação que regulamenta a comercialização de alimentos e ingredientes contendo OGMs é totalmente dependente da sensibilidade e confiabilidade dos métodos de detecção e quantificação de OGMs. . Entre os métodos existentes, a reação em cadeia da polimerase (PCR) é o mais aceito, por ser sensível e confiável para detecção de material geneticamente modificado em análises de rotina. A padronização da técnica de PCR multiplex para a detecção de vários transgênicos em produtos alimentícios facilita o monitoramento destes, pois além de diminuir o tempo de diagnóstico, utiliza uma menor quantidade de reagentes, barateando o processo de detecção. No presente estudo, foi utilizada uma duplex PCR para a detecção de soja geneticamente modificada tolerante ao herbicida glifosato (Roundup Ready) e uma PCR multiplex para a detecção simultânea de três linhagens de milho transgênico (MON810, Bt11 e Bt176) em amostras de alimentos humano e animal. Foram utilizadas duas metodologias para extração do DNA das amostras: o método CTAB (já utilizado pelo INCQS) e o kit DNeasy Mini. [...]
From 1996 to 2006, the proportion of the global area of biotech crops growing in developing countries has increased consistently. In the last ten years, over 40 countries have adopted policies for the labeling of genetically modified food, but the regulations characteristics and their degree of implementation varies greatly. The effects of these policies on consumer choice and information, on food trade and international trade also vary significantly. In Brazil, the Decree No. 4.680/03 regulates the right to consumers information, provided by Law No. 8078/90, on food and food ingredients intended for human or animal consumption containing or produced from genetically modified organisms (GMOs). Compliance with regulation on the marketing of foods and ingredients containing GMOs is totally dependent on the sensitivity and reliability of methods of detection and quantification of GMOs. Among the existing methods, the polymerase chain reaction (PCR) is the most accepted, sensitive and reliable for the detection of genetically modified material in routine. The multiplex PCR is used on the screening of GMO in food because its able to detect simultaneously several transgenic, being a less time and reagents consuming method, making the process of detection cheaper. In the present study, duplex PCR was tested for the detection of genetically modified soybeans tolerant to the herbicide glyphosate (Roundup Ready) and a multiplex PCR was tested for the simultaneous detection of three lineages of transgenic maize (MON810, Bt11 and Bt176) in samples of human food and feed. Two methodologies were used for extraction of DNA from samples: the CTAB method (already used by INCQS) and DNeasy® Mini kit. The results were compared to the standard method used by INCQS. Out of the fourty samples, thirty samples were positive for the presence of Roundup Ready soybeans, fourteen were positive for at least one of the GM corn studied. Nine were positive for MON 810 maize, three were positive for Bt 11 maize, and five were positive for Bt 176 maize. The method proved to be robust and can be used on a routine for the detection of GMOs in food.
Teixeira, Ana Izabel Passarella. "Desenvolvimento de uma PCR multiplex para identificação de clostrídios histotóxicos". Universidade Federal de Minas Gerais, 2012. http://hdl.handle.net/1843/BUOS-97BHV2.
Texto completoA reação em cadeia da polimerase (PCR) tem se destacado nos últimos anos como uma importante técnica para identificação de microrganismos por ser extremamente sensível e versátil. Porém, até então não existia uma PCR Multiplex que fosse capas de identificar os cinco agentes etiológicos causadores de mionecroses clostridiais: Clostridium septicum, C. chauvoei, C. novyi tipo A, C. perfringens tipo A e C. sordellii. Portanto, nesse trabalho foi desenvolvida uma PCR Multiplexpara a detecção e discriminação dos clostrídios histotóxicos. A sensibilidade analítica obtida foi de aproximadamente 0,15 ng para C.chauvoei e 0,015 ng para outros agentes. Não aconteceram amplificações no teste de especificidade com as espécies Clostridium tetani(ATCC 9441), Staphylococcus aureus (ATCC 27707), Pseudomonas aeruginosa (ATCC 25319), Escherichia coli (ATCC 21986), Clostridium difficile (ATCC 9689), Proteus mirabillis (ATCC 12453) e Salmonella typhi (ATCC 9992V) , confirmando-a. Este trabalho apresenta uma PCR Multiplex eficientee especifica para detecção e identificação de clostrídios histotóxicos.