Tesis sobre el tema "Mud crabs"
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Churchill, Giles John. "An investigation into the captive spawning, egg characteristics and egg quality of the mud crab (Scylla serrata) in South Africa". Thesis, Rhodes University, 2004. http://hdl.handle.net/10962/d1015963.
Texto completoPavasovic, Marko. "Digestive profile and capacity of the mud crab (Scylla serrata)". Thesis, Queensland University of Technology, 2004. https://eprints.qut.edu.au/15973/1/Marko_Pavasovic_Thesis.pdf.
Texto completoPavasovic, Marko. "Digestive profile and capacity of the mud crab (Scylla serrata)". Queensland University of Technology, 2004. http://eprints.qut.edu.au/15973/.
Texto completoGopurenko, David y n/a. "Genetic Structure Within the Distribution of the Indo-West Pacific Mud Crab Scylla serrata (Forskal, 1775)". Griffith University. Australian School of Environmental Studies, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20030926.124631.
Texto completoHill, Jennifer Marie. "Predator biomass and habitat characteristics affect the magnitude of consumptive and non-consumptive effects (NCEs): experiments between blue crabs, mud crabs, and oyster prey". Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/41172.
Texto completoTruong, Phuong Ha. "Nutrition and feeding behaviour in two species of mud crabs Scylla serrata and Scylla paramamosain". Thesis, Queensland University of Technology, 2008. https://eprints.qut.edu.au/20700/1/Phuong_Truong_Thesis.pdf.
Texto completoTruong, Phuong Ha. "Nutrition and feeding behaviour in two species of mud crabs Scylla serrata and Scylla paramamosain". Queensland University of Technology, 2008. http://eprints.qut.edu.au/20700/.
Texto completoOverton, Julia Lynne. "Morphometric, genetic and reproductive characteristics of mud crabs (genus Scylla de Haan, 1833) from Southeast Asia". Thesis, University of Stirling, 2000. http://hdl.handle.net/1893/2599.
Texto completoGopurenko, David. "Genetic Structure Within the Distribution of the Indo-West Pacific Mud Crab Scylla serrata (Forskal, 1775)". Thesis, Griffith University, 2003. http://hdl.handle.net/10072/367817.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
Australian School of Environmental Studies
Full Text
Vu, Ngoc Ut. "Assessment of the feasibility of stock enhancement of mud crabs, Scylla paramamosain, in the Mekong Delta, Vietnam". Thesis, Bangor University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273571.
Texto completoLebata, Ma Junemie Hazel L. "Stock enhancement of the mud crabs Scylla spp. in the mangroves of Naisud and Bugtong Bato, Ibajay, Aklan, Philippines". Thesis, Bangor University, 2006. https://research.bangor.ac.uk/portal/en/theses/stock-enhancement-of-the-mud-crabs-scylla-spp-in-the-mangroves-of-naisud-and-bugtong-bato-ibajay-aklan-philippines(76e61aca-da56-412c-8e71-6e8a14f37877).html.
Texto completoConnolly, Lauren E. "Effect of predator diet on foraging behavior of panopeus herbstII in response to predator urine cues". Thesis, Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/53392.
Texto completoMirera, David Oersted. "Capture-based mud crab (Scylla serrata) aquaculture and artisanal fishery in East Africa- Practical and ecological perspectives : Mud crab ecology and aquaculture". Doctoral thesis, Linnéuniversitetet, Institutionen för biologi och miljö (BOM), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-32399.
Texto completoWebley, James A. C. "The ecology of the mud crab (Scylla serrata): their colonisation of estuaries and role as scavengers in ecosystem processes". Thesis, Griffith University, 2008. http://hdl.handle.net/10072/367091.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environment
Science, Environment, Engineering and Technology
Full Text
Nguyen, Thi Bich Ngoc. "Commercial mud crab Scylla Serrata : Study on growth, energy and protein requirement of juveniles in the view to develop peleted feed for crab farming in New Caledonia". Thesis, Nouvelle Calédonie, 2014. http://www.theses.fr/2014NCAL0057/document.
Texto completoIn New Caledonia, there is the strong political will to diversify aquaculture which is mainly based on shrimp farming. In this context, mud crabs have been considered as a potential species for aquaculture development. One of the main constraints to develop crab farming is the availability of formulated feed. Thus, the main purpose of this thesis is to get information on the crab nutritional requirements in order to formulate a balanced diet. However, we had to clarify first how many species of mud crab were present in New-Caledonia. The result of our morphological and genetic investigations carried out on 63 specimens from 9 areas of the west and northeast coast of New-Caledonia confirmed that only one species, Scylla serrata, is commercialized in this country. Consequently, S. serrata was used in our nutritional study based on two experiments to: i) evaluate the soy protein concentrate (SPC) compared with the fishmeal as the main protein source and ii) determine the optimum level of SPC in the diet for molting and tissue growth. We observed two tissue growth phases within one molt cycle (MC): a fast tissue growth (FTG) occurred after ecdysis until early intermolt stage (30% of MC) which is followed by a slow tissue growth (STG) period from intermolt to ecdysis (70% of MC). Protein and lipid deposition followed the same trend than tissue growth while ash level increased quickly during five days after molt and then remained stable until the next molt. The two growth phases were correlated with the voluntary feed intakes (VFI) which was maximum during 2 weeks after ecdysis and then decreased by 50% over the five following weeks to reach a baseline until the next molt. Intake energy was allocated mainly for growth during FTG period and for maintenance during STG period. During STG, 28% of the ingested energy was accumulated for the next ecdysis. Replacement of fishmeal by SPC as main protein source did not affect tissue growth, efficiency of feed utilization and energy budget of crabs whatever the tissue growth period considered. The dietary SPC inclusion of 42% in the diet promoted growth (molt frequency and tissue growth), feed efficiency and retention of energy, protein and lipid. Hypothesis related to ammonia toxicity from catabolism of proteins in excess or anti-nutritional factors from soybean could explain the negative effects of higher inclusion of SPC in the diet for juvenile crabs. In conclusion, our work brings novel information on tissue growth, energy budget during a molt cycle and the ability of juvenile crab to use SPC as a main source of protein. On this basis we suggest to formulate nutritionally balanced diet without fishmeal to farm juvenile mud crabs S. serrata
Le, Van Chi. "Genetic resources in wild and cultured stocks of the Asian mud crab, Scylla paramamosain". Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/39232/1/Van_Chi_Le_Thesis.pdf.
Texto completoSanoara, Yasmin. "Enhancing the Sustainable Livelihood of Crab Fishers: A Study of the Mud Crab Value Chain of Coastal Bangladesh Using the Social Business Model". Thesis, Curtin University, 2018. http://hdl.handle.net/20.500.11937/70493.
Texto completoOng, Moc Quy. "Supplementing probiotics during early stages of mud crab (Scylla paramamosain) culture under various rearing systems". Thesis, Curtin University, 2018. http://hdl.handle.net/20.500.11937/78345.
Texto completoRoche, Dominique. "Discovery, distribution, and eradication potential of the introduced mud crab, «Rhithropanopeus harrisii», in the Panama Canal". Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=32379.
Texto completoL'éradication d'espèces introduites invasives est un concept populaire dans le domaine de la biologie de la conservation. Lorsque la prévention d'une invasion échoue, de nombreux écologistes soutiennent que l'éradication est la méthode la plus efficace afin d'éviter que les espèces introduites ne causent de dommages, considérant les coûts élevés et les effets parfois prolongés du contrôle à long-terme. Notamment, dans la majorité des systèmes, le succès d'un programme d'éradication est supérieur s'il est entrepris tôt lors du processus d'envahissement, lorsqu'une espèce est peu dispersée. Cependant, cette stratégie qui consiste à éradiquer les espèces introduites de façon hâtive est rarement mise en pratique. Ce paradoxe peut résulter d'un haut niveau d'incertitude concernant le potentiel qu'ont les espèces nouvellement introduites à se propager et à causer des impacts, et par le fait que les administrateurs responsables de leur gestion manquent souvent de conseils pratiques afin de réagir promptement suite à leur découverte. Pour remédier à cette lacune, cette thèse se base sur une importante étude de cas et suggère un modèle théorique servant à analyser de façon rapide la possibilité d'implémenter un programme d'éradication hâtif et d'en évaluer les bénéfices. Premièrement, je documente la découverte récente du crabe Rhithropanopeus harrisii dans le Canal de Panama. Le Canal de Panama est une plaque tournante du trafic maritime international et pourrait jouer un rôle important dans l'accroissement de la propagation de R. harrisii au niveau mondial. Deuxièmement, au moyen d'un échantillonnage quantitatif stand
Crosby, Chelsea Helene. "The role of individual learning and dietary preference in the consumption of the invasive Green Porcelain Crab, Petrolisthes armatus, by Native Crab Predators". The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1523887793938929.
Texto completoGrogan, Whitney Nicole. "A mid-Atlantic study of the movement patterns and population distribution of the American horseshoe crab (Limulus polyphemus)". Thesis, Virginia Tech, 2004. http://hdl.handle.net/10919/36327.
Texto completoMaster of Science
"Studies of androgenic gland in the mud crab: scylla paramamosain". 2004. http://library.cuhk.edu.hk/record=b6073733.
Texto completo"September 2004."
Thesis (Ph.D.)--Chinese University of Hong Kong, 2004.
Includes bibliographical references (p. 241-269)
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Mode of access: World Wide Web.
Abstracts in English and Chinese.
Liessmann, Laurence. "Investigation into the mortalities of larval mud crabs, Scylla serrata and methods of control". Thesis, 2005. https://researchonline.jcu.edu.au/17553/1/01front.pdf.
Texto completoChen, Hong-Yu y 陳鴻裕. "Study on biochemical characteristics of hemocyanins in mud crab Scylla olivacea". Thesis, 2006. http://ndltd.ncl.edu.tw/handle/55831306241620943255.
Texto completo國立中山大學
海洋生物科技暨資源學系研究所
94
Hemocyanin, a copper containing protein, is the respiratory protein of Crustacea. Here, 4 major hemocyanin molecules in the hemolymph of Scylla olivacea were purified and studied. Both two-hexamer (24S) and hexamer (16S) form of hemocyanin are present in all adult’s hemolymph. In vitro, 24S hemocyanin dissociates into two 16S hemocyanin in the absence of calcium under high pH value (> 8.9). Little of 16S hemocyanin assembles spontaneously into 24S hemocyanin after calcium is added. Both 16S and 24S hemocyanins were purified from hemolymph, the 16S hemocyanin differs from the 24S hemocyanin in the presence of intersubunit disulfide bonds, it is inferred that not all native 16S hemocyanin dissociates from 24S hemocyanin. Besides the 24S and 16S hemocyanin, there are two massive proteins in the hemolymph of ovary-maturing female S. olivacea. One is female-specific hemocyanin (FSH), which occurs in the hemolymph of ovary-maturing females, but not in the hemolymph of juveniles of either sex or in adult males. FSH is confirmed as a hemocyanin due to its copper content and oxygen binding ability. FSH is also found in ovary, embryo and early-stage zoea, and is proposed to be an important hemocyanin that supplies enough oxygen for ovary-maturing female crab, egg, embryo and early-stage zoea. Another massive hemolymph protein is a non-respiratory protein (NRP) that is present in hemolymph of adults of both sex, the molecular mass of its subunits is similar to hemocyanin and FSH, and one of subunits can be slightly recognized by anti-FSH antibody. NRP is not a respiratory protein, there is neither copper containing nor absorbance of 340nm. FSH and NRP, especially NRP, contain more carbohydrates than the 16S hemocyanin.
Zhang, Zhong Zuo y 張仲佐. "Studies on protein and energy for juvenile mud crab, scylla serrata". Thesis, 1994. http://ndltd.ncl.edu.tw/handle/13934960557113483504.
Texto completoGenodepa, Jerome. "Development of a formulated diet for mud crab, Scylla sewata, larvae". Thesis, 2004. https://researchonline.jcu.edu.au/1262/2/01abstract.pdf.
Texto completo陳文彬. "Effect of Salinity Acclimation on Chemical Constituents of mud crab (Scylla serrata)". Thesis, 2002. http://ndltd.ncl.edu.tw/handle/85739719645374943956.
Texto completo國立海洋大學
食品科學系
90
Abstract The purpose of this study was to investigate the changes of chemical constituents in the abdominal muscle of female mud crab in relation to short-term salinity acclimation and subsequent air-exposured storage at 15℃. The crab specimens were acclimated to 25 ppt of sea water for 24 hours (control) and then transferred to 10 and 40 ppt sea water for a period of 4~48 hours. In comparison with control, moisture in 40 ppt sample decreased, but that in 10 ppt sample increased. On contrary, the glycogen showed a inverse trend. Taurine (Tau), glutamine (Gln), proline (Pro), glycine (Gly), alanine (Ala) and arginine (Arg) were dominant free amino acids (FAA) and they together accounted for about 87﹪of the total FAA. Adenosine triphosphate (ATP) and glycinebetaine (GB) were respectively the major component among the ATP-related compounds (ARC) and quternary ammonium bases (QAB) detected. As a whole, the 40 ppt sample was relatively high in levels of the total FAA, total ARC, GB, and homarine (Hom). In addition, the difference in levels of moisture, glycogen, total FAA, total ARC, GB, and Hom between 10 and 40 ppt samples increased with the elongation of acclimation time. The crab specimens acclimated to 10 ppt, 25 ppt, and 40 ppt sea water and then stored at 15℃ for 12~72 hours. Differences in the moisture and glycogen levels declined after storage. The total FAA in all samples elevated gradually with the storage time and the sample acclimated to higher salinity was more pronounced. The major FAA except for Tau also increased. After storage, the total ARC content was higher in the 40 ppt sample than in other two samples. The ATP decreased with storage time, while its breakdown products accumulated. The GB and Hom contents were higher in the sample acclimated to higher salinity, but their difference between the samples lowered as the storage time increased. Of the samples acclimated to 10、25, and 40 ppt sea water for 48 hours, their hot-water extracts were prepared and compared by sensory test for taste. Taste intensity of the extracts was higher in the 25 and 40 ppt samples than in the 10 ppt sample. The salt, Na+, and total FAA contents in the hot-water extracts followed the order of 40 ppt > 25 ppt > 10 ppt sample. Levels of glycogen, GB, and Hom were higher in the 25 ppt sample than in the 40 ppt and 10 ppt samples. However, there was no significant difference in the total amounts of ARC between them.
Chi-YuanSu y 蘇啓元. "Benefit Assessment of Mud Crab Short-term Culture for Taiwanese Seafood Restaurant". Thesis, 2015. http://ndltd.ncl.edu.tw/handle/88089915703055270928.
Texto completo國立成功大學
海洋科技與事務研究所
103
In Taiwanese dietary habit, mud crab dishes are one of the most delicious food in autumn. According to the data from Fisheries Agency in Taiwan, the average import quantum of mud crab is about 4000 tons in the last decade. Living mud crab is the main import item with more than 80% amount other imported crab. High demand for mud crab is catachrestic during the period from September to December, while the harvest of mud crab in Southeast Asia is lower than in summer. Therefore, the demand and supply are usually inconsistent. . Demand of mud carb is also various in short periods that demand in weekend and holiday is rapidly rising than week days. This study thus aims to reach the rapidly raised demand of mud crab by short-term culturing with economic assessment. In this study, mud crabs are cultured for a period of time by a small-scale culturing tank. The survival rate of mud crabs is examined to compare with the result of questionnaires that sent to 20 seafood restaurants. According to the result of questionnaires, the mud crab of cultured by these sea food restaurants has a mortality rate about 15% which is lower that of nonculture restaurants. The result of small-scale culturing experiment in this study shows that the initial mortality rate about 15% in restaurants of owning culture tank could be reduced to 5% by using the culturing system designed in this study. In addition, the benefit assessment of short-term culture of mud carbs in one month revealed that profit of a restaurant would increase about 13% while the mortality rate is lower than 5% and can avoid the magnetic effect from China.
(9777185), Leonie Andersen. "A study into the epidemiology of mud crab (Scylla serrata) shell disease". Thesis, 2003. https://figshare.com/articles/thesis/A_study_into_the_epidemiology_of_mud_crab_Scylla_serrata_shell_disease/13417376.
Texto completoCheng-Yen, Chang y 張政彥. "Structural and functional analyses of a crustacean hyperglycemic hormone in the mud crab Scylla olivacea". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/75021724172716558412.
Texto completo國立彰化師範大學
生物學系
98
Sco-CHH and Sco-CHH-L (CHH-like peptide), two structural variants of the crustacean hyperglycemic hormone family identified in the mud crab (Scylla olivacea), are identical up to the 40th residue, but different from each other in the remaining sequence. Analyses of transcript sequences indicated they are encoded by alternatively spliced transcripts of the chh gene. Recombinant protein rSco-CHH were successfully produced using an E. coil expression system, refolded, purified, and confirmed by Western blotting, mass spectrometric analyses, circular dichroism analyses and subsequently used to confer the function of Sco-CHH. To reveal the structural bases of functional divergence, site-directed mutagenesis was used to produced mutated rSco-CHH (mutated sites located at 2, 3, 4, 12, 13, 51, 54, 60, 69, 70, 72 amino acid of the mature Sco-CHH) and tested the biological activity by using the established bioassays. The hyperglycemic activity of I2G (The second amino acid of mature Sco-CHH isoleucine mutated to glycine) decreased by 54.9 ± 50.5 %, F3A (phenylalanine mutated to alanine) decreased by 38.2 ± 57.9 %, Q70A (glutamine mutated to alanine) decreased by 27.1 ± 47.7 %, and V72G (valine mutated to glycine) decreased by 34.1 ± 45.9 %. The substitutions of critical residues at the N-terminus and C-terminus inferred that the N and C-terminal hydrophobic activity of Sco-CHH was important to receptor binding. Furthermore, D12N (aspartic acid mutated to asparagine) lost the hyperglycemic activity. Q51A (glutamine mutated to alanine) and E54Q (glutamic acid mutated to glutamine) not only decreased hyperglycemic activity but also changed the secondary structure of Sco-CHH. The result indicated that these critical residues contributed to the stability of Sco-CHH. The combined results support the notion that Sco-CHH and Sco-CHH-L are functionally divergent, and might reveal the structural bases of functional divergence.
Tsou, Li-Tse y 鄒禮澤. "The classification and non-specific immune functions of the haemocytes of the mud crab (Scylla serrata)". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/70550779573927340900.
Texto completo國立中興大學
獸醫學系暨研究所
95
The aim of this study was to investigate the morphology and haemocytic immune functions of haemocytes in mud crab (Scylla serrata). The effects of immunostimulants on mud crab haemocytes were also studied in vitro. Our results revealed that flow cytometry could successfully classify the crab’s haemocytes into three populations: hyalinocytes, semi-granulocytes and granulocytes. For young female crabs, the percentages of hyalinocytes, semi-granulocytes and granulocytes were 33.70%, 43.47%, and 5.06%, respectively. Because the percoll gradient centrifugation method could not clearly distinguish each subpopulation, we applied flow cytometry to differentiate among the different groups of haemocytes. Our data showed that our technique was capable of separating the haemocytes. The purities of hyalinocytes, semi-granulocytes and granulocytes were 83.1%, 86.8% and 76.9%, respectively. The morphology of the three haemocytes were observed by optical microscopy, fluorescence microscopy, and TEM, respectively. In this study, eight kinds of immunostimulants, including five types of glucan (laminarin, levan, scleroglucan, yeast glucan, and zymosan) and three different sources of lipopolysaccharide (LPS) from Escherichia coli, Pseudomonas aeruginosa and Salmonella enterica were used. Our data suggested that the working dosage of yeast glucan, zymosan, LPS E and LPS P required to induce the crab’s innate immune responses was 20 μg/mL. Interestingly, we found that immunostimulants could change the proportions of the crab’s haemocytic subpopulations, especially for hyalinocytes which increased markedly. The percentages of semi-granulocytes and granulocytes, however, decreased. In order to investigate these changes, a cell proliferation assay was conducted. There were no apparent increases in haemocytic subpopulations when cultured with glucan or LPS. Our data suggested that the cause of changes in haemocytic proportion was subpopulation shift by degranulation, rather than by cell proliferation. Additionally, we confirmed that prophenoloxidase (proPO) was located in haemocytes but not in serum. Moreover, proPO activity could be induced by SDS, trypsin, zymosan, and LPS E. However, it was inhibited by soybean trypsin inhibiter. According to respiratory burst reaction and phagocytosis assay, our results indicated that granulocytes had the strongest phagocytosis and respiratory burst abilities among the three haemocytes. Hyalinocytes had very low phagocytosis and respiratory burst abilities. The abilities of semi-granulocytes were lower than those of granulocytes but higher than those of hyalinocytes. Our results showed that the innate immune response of haemocytes was capable of being enhanced by exposure to high concentrations of bacteria. We found their survival rate as well as respiratory burst and phagocytosis abilities were significantly increased after the cells were treated with immunosimulants (P<0.05).
Liu, Chun-Jun y 劉純君. "A study of functionally critical amino acid of crustacean hyperglycemic hormone in the mud crab Scylla olivacea". Thesis, 2013. http://ndltd.ncl.edu.tw/handle/94130506368485113690.
Texto completo國立彰化師範大學
生物學系
101
Crustacean hyperglycemic hormone (CHH) is a polypeptide hormone belonging to the CHH family that includes, in addition to CHH, several other members. CHH is involved in maintaining basal hemolymph glucose levels and mediating stress-induced hyperglycemia. In the present study, an approach of alanine-scanning mutagenesis was employed to determine the residues important for the hyperglycemic activity of CHH. CHH of the mud crab Scylla olivacea (rSco-CHH-Gly) and point-mutated (I2A, F3A, D4A, D12A, R13A, E54A, D60A, I69A, or V72A ) rSco-CHH-Gly were produced as glycine-extended recombinant proteins using an Escherichia coli expression system, refolded, purified, the identities of which were confirmed by mass spectrometric and circular dichroism (CD) analyses. CD spectra of mutant rSco-CHH-Gly indicate they are rich in α-helixes. The different mutated rSco-CHH-Gly was C-terminally amidated (except the V72A rSco-CHH-Gly), and tested for biological activity using an in vivo hyperglycemic assay. Results showed that the hyperglycemic activities of R13A, I69ArSco-CHH and V72ArSco-CHH-Gly completely lost their biological activity. I2A, F3A, D12A, D60ArSco-CHH had hyperglycemic activity but significant different compared to that of the wild type rSco-CHH. Hyperglycemic activity of D4A, E54A rSco-CHH were similar to wild type rSco-CHH. Finally,in co-injection bioassay results suggested that were unable to block R13A, I69A rSco-CHH or V72A rSco-CHH-Gly from CHH induced hyperglycemic. Taken together, the results show tha several amino acids located at the N and C terminal of CHH affect the hyperglycemic activity. In particular, residues located at the C-terminal helix are critical in that mutations at these sites lend to complete loss of biological activity.
Chen, Yun-Ru y 陳韻如. "Structural and Functional studies of Crustacean Hyperglycemic Hormone-like (CHH-L) Peptide from Mud crab Scylla Olivacea". Thesis, 2018. http://ndltd.ncl.edu.tw/handle/mrfm3q.
Texto completoHolme, May-Helen. "Towards development of a formulated diet for mud crab (Scylla serrata) larvae, with emphasis on lipid nutrition". Thesis, 2008. https://researchonline.jcu.edu.au/2150/1/01front.pdf.
Texto completoChen, Chao-Ying y 陳昭穎. "Changes in the hemocyanin structure during vitellogensis, embryo and larval development of red-claw mud crab, Scylla olivacea-". Thesis, 1998. http://ndltd.ncl.edu.tw/handle/96557156748550288093.
Texto completo國立中山大學
海洋生物研究所
86
The hemocyanin during vitellogenesis, embryo and larval development wasstudied in the red-claw mud crab, Scylla olivacea. After purified from ion exchange chromatography, subunit composition was analyzed using SDS-PAGE. Inaddition, rabbit antisera were prepared by injections of the purified hemocyanin. These antisera were used to compare the difference among each type of hemocyanin.Two hemocyanin forms, hexamer and dodecamers were found in native-PAGE of hemolymph samples from males and immature females. An additional female specificprotein (FSP) was found in the hemolymph from mature female. When gel bands were tested for the presence of copper, FSP and other hemocyanins all gave pos-itive reactions. The female specific proteinnot only exited in the hemolymph of mature female, but also in ovary and embryoand first zoeal instar. Morever, FSP was the only hemocyanin type in ovary andembryo tissue. Besides, the FSP contain increased in both hemolymph and oocytes as the ovary maturing but decreasedin embryos as embryo developing. It is suggested, FSP is synthesized in tissuesoutsides the ovary and stored in oocytes through hemolymph by endocytosisduring vitellogesis and then degenerated or changed to other hemocyanin formsduring embryonic development. The composition of three form hemocyanins from mature female was analyzed by SDS-PAGE. The dodecamers showed six distinct subunits and FSP and hexamerswere composed of only five of the six subunits, both luck subunit a. The presence of subunit a in dodecamers is consistent withthe idea that subunita is necessary for the formation of the two-hexamer aggregate.Immunoprecipitationreaction of three hemocyanin types was tested with antiserumagainst female specific protein and hemocyanin. It showes that the FSPand hexamer had thehigher cross reaction thus they may have similar molecularstructure. In addition,the subunit composition of isolated polymeric forms ofhemocyanin was analyzed by crossed immunoelectrophoresis. The dodecamers revealed four precipitation peaks-I, II, III and IV, but FSP and hexamers contained only three precipitation peaks. These results suggest that the subunit IV is required as a joining piece for the assembly of dodecamers.-1 -aChanges in the hemocyanin structure during vitellogensis, embryo and larval development of red-claw mud crab, Scylla olivacea
Xie, Ping Yu y 謝平玉. "Effects of different salinity and temperature levels on nitrogen metabolism and ions regulation of mud crab scylla serrata". Thesis, 1994. http://ndltd.ncl.edu.tw/handle/22767177718392243400.
Texto completoTsai, Kuo-Wei y 蔡國瑋. "Comparative studies on molecular characterizations and physiological functions of crustacean hyperglycemic hormone isoforms in the mud crab Scylla olivacea". Thesis, 2005. http://ndltd.ncl.edu.tw/handle/13309963145382498785.
Texto completo國立彰化師範大學
生物學系
93
Crustacean hyperglycemic hormone (CHH) is a neuropeptide original identified from the X-organ (XO)-sinus gland (SG) complex in the crustacean eyestalk. In the present work, three full-length CHH cDNAs were cloned from the mud crab Scylla olivacea eyestalk ganglia (ES), thoracic ganglia (TG), and per- icardial organ (PO) by RT-PCR and rapid amplification of cDNA end (RACE). PO-CHH and TG-CHH have the same deduced amino acid sequence. TG/PO-CHH and sinus gland CHH (SG-CHH) share an identical N-terminal sequence (amino acids 1-40), but the remaining sequences (amino acids 41-73 or 41-75), differ considerably. The sequence evidence suggests that these two isoforms are derived a Chh gene transcribed in an alternative splicing manner. With the use of a two-step HPLC purification procedure and a CHH isoform- specific ELISA, the two CHH isoforms were isolated from sinus gland and pericardial organ, respectively. Mass spectrometric analysis indicates that the molecular masses for the native SG-CHH and TG/PO-CHH are 8379 Da and 8478 Da, respectively, which are consistent with the estimated values based on the deduced sequences. It also suggests that the two CHHs have a blocked N- terminus, and the C-terminus of SG-CHH is amidated, but TG/PO-CHH has a free C-terminus. The partial amino acid sequences of SG-CHH and TG/PO- CHH were determined by MALDI MS/MS analysis of trypsin-digested peptide fragments. The results show that the amino acid sequences of these peptide fragments were identical to the deduced amino acid sequences of the SG-CHH and TG/PO-CHH cDNAs. In addition, SG-CHH (10 pmole) exhibited a hyper- glycemic activity in vivo, whereas the same dosage of TG/PO-CHH did not elicit a hyperglycemic response. The physiological function of TG/PO-CHH remains to be clarified. We speculate that TG/PO-CHH may have regulatory functions differ from those of SG-CHH.
Genodepa, Jerome G. "Digestive enzyme dynamics during early life stages of the mud crab, Scylla serrata and the spiny lobster, Panulirus ornatus". Thesis, 2015. https://researchonline.jcu.edu.au/46242/1/46242-genodepa-2015-thesis.pdf.
Texto completoChang, Chih-Chun y 張智鈞. "Expression, characterization, and function of two structural variants of the crustacean hyperglycemic hormone family in the mud crab Scylla olivacea". Thesis, 2008. http://ndltd.ncl.edu.tw/handle/45003090455562052453.
Texto completo國立彰化師範大學
生物學系
96
Sco-CHH and Sco-CHH-L (CHH-like peptide), two structural variants of the crustacean hyperglycemic hormone family identified in the mud crab (Scylla olivacea), are identical up to the 40th residue, but different from each other in the remaining sequence. In this study, recombinant proteins (rSco-CHH and rSco-CHH-L) were produced by an E. coil expression system, refolded, purified, and confirmed by Western blotting and mass spectrometric analyses. Purified rSco-CHH was C-terminally amidated (rSco-CHHamide) in accordance with its native counterpart. Circular dichromatic spectra of rSco-CHHamide and rSco-CHH-L indicate they are rich in -helixes (47 % and 46 %, respectively); mass spectrometric analyses of peptide fragments of rSco-CHH and rSco-CHH-L reveal a common disulfide bond pattern typical of CHH family peptides. Functionally, rSco-CHHamide at 10 or 100 pmoles/animal elicited significant hyperglycemic responses, whereas rSco-CHH-L had no effect at the same dosage. On the other hand, rSco-CHH-L, but not rSco-CHHamide, dose-dependently (0-400 nM) increased the gill Na+/K+-ATPase activity. Finally, gene expression assays showed that the levels of Sco-CHH-L, but not Sco-CHH, were significantly increased in acclimated animals 12 h after exposing to an osmotic stress. In summary, recombinant Sco-CHH and Sco-CHH-L were successfully produced and characterized. Studies using the recombinant proteins provide evidence revealing that the 2 CHH structural variants diverge functionally.
LIN, HUI-ZHEN y 林惠真. "The reproductive behavior and mate choice of fiddler crab, uca lactea lactea, in mid-Taiwan". Thesis, 1986. http://ndltd.ncl.edu.tw/handle/76715711691696753006.
Texto completoLin, Jong Huzy y 林仲惠. "Studies on the taxonomic status and population genetics of the mud crab Scylla serrata (Crustacea:Decapoda:Brachyura) by using morphometric,biochemical and behavioral methods". Thesis, 1993. http://ndltd.ncl.edu.tw/handle/78828930849703898201.
Texto completoLin, Yea-Jen y 林雅真. "Responses of juvenile mud crab (Scylla serrata) to different oil sources and different levels of n-3 or n-6 polyunsaturated fatty acids". Thesis, 1996. http://ndltd.ncl.edu.tw/handle/34277339521722223425.
Texto completo國立海洋大學
水產養殖學系
84
This study was conducted to investigate the effects of diets with different sources of lipid and different quantity of polyunsaturated fatty acids (PUFA) or highly unsaturated fatty acids (HUFA) on the growth and survival of juvenile crabs. In Experiment 1, juvenile crabs were fed with diets supplemented with 4 % cod liver oil, corn oil, olive oil, soybean oil and sesame oil for 10 weeks. The results showed that juvenile crabs fed with 4 % cod liver oil had the best growth. Juvenile crabs fed with diets containing corn oil, olive oil, soybean oil and sesame oil showed no significant differences. Juvenile crabs fed diets containing cod liver oil had high level of EPA and DHA of body tissue of juvenile crabs. These results showed that the fatty acid composition of tissue of crabs was obviously reflected from fatty acid composition of diet. Weight gain and survival responses and analyses of fatty acid composition of tissue were used to investigate the nutritional value of C18 polyunsaturated fatty acids (PUFA) provided to juvenile crabs in a purified diet. There were seven isonitrogenous and isoenergetic diets containing pure triglycerides of 0.2, 0.4 and 0.6﹪18:3n-3 (trilinolenin) and 18:2n-6 (trilinolein) and 0.2 ﹪22:6n-3 (tridocosahexaenoin) as control diet for experiment 2 . All diets were formulated to contain a total of 4﹪lipid and were fed to juvenile crabs for 12 weeks. The results showed that juvenile crabs fed diets containing 0.2﹪tridocosahexaenoin showed better growth than those fed diets containing trilinolenin and trilinolein. Diets containing high proportion of trilinolein or trilinolenin could not improve the growth of juvenile crabs. The high level of 18:2n-6 and 18:3n-3 in the polar and neutral lipids of tissue of juvenile crabs was found that crabs were fed diets containing trilinolein or trilinolenin. Crab fed diet containing 0.2 % tridocosahexaenoin had high level of EPA and DHA in the polar lipid of crab tissue. Weight gain and survival responses and analyses of fatty acid composition of tissue were used to investigate the nutritional value of C≧20 highly unsaturated fatty acids (HUFA) provided to juvenile crabs in a purified diet. There were isonitrogenous and isoenergetic diets containing pure triglycerides of 0.2, 0.4 and 0.6﹪20:4n-6 (triarachidonin) and 22:6n-3 (tridocosahexaenoin). Reference diet was included cod liver oil as the lipid source. All diets were formulated to contain a total of 4﹪lipid. A diets containing only saturated (14:0, 16:0, and 18:0) and monounsaturated (16:1n-7 and 18:1n-9) fatty acids as lipid sources, in proportions similar to that found in a 2:1 cod liver oil/corn oil mixture, served as a conditioning and control diets for experiment {= 3 \* ROMAN|III}. At the termination of the feeding trials, that juvenile crabs fed diets containing 0.4 % tridocosahexaenoin and 4 % cod liver oil had the best growth. The control group (diet included without PUFA and HUFA) had the lowest growth and survival. Crab fed diets containing 0.6 % triarachidonin and control group had high mortality. Crab fed diets containing triarachidonin (20:4n-6) and tridocosahexaenoin (22:6n-3) had high level of 20:4n-6 and 22:6n-3 in the tissue. However, crab fed diets containing tridocosahexaenoin (22:6n-3) had high level of EPA and DHA in the polar lipid of crab tissue.
LIN, WEI-CHI y 林韋齊. "The effect of environmental factors and adjacent land use of wetland on crab community in mid-Taiwan". Thesis, 2016. http://ndltd.ncl.edu.tw/handle/62582228376049791544.
Texto completo東海大學
生命科學系
104
The biodiversity of estuarine wetlands was affected by natural process and human disturbance, such as seawall construction, pollution, and tidal effect. Many kinds of data were used to be indicators for quantifying the quality of wetland, including water quality, the biodiversity or composition of birds, fishes, invertebrates, or condition of coastline. Among the biodiversity indices, Abundance Biomass Comparison Method (ABC method) is used to detect the effect of environmental stress on marine macro benthic communities and was widely used on fish or benthic community in recent years. ABC method was applied to one of the previous study in our lab in 2003 and, in that study, the crab communities in Da’an, Gaomei, and Dadu wetlands were all under moderately stressed. But the environmental factors were not included in the discussion of that study. Therefore, the first part of this study was to exam the association between environmental factors and crab community. The second part of this study is to assess the environmental stress on the three wetlands in ten years through analyzing the difference of crab community in different year. Biotope Area Factor (BAF) was widely used in landscape ecology. In BAF, it is hypothesized that different land uses were weighed by the permeability and vegetation cover ratio. When the surface has a higher permeability or more vegetation covered, a higher ecologically-effective weighting will be given. Therefore, BAF may be applied to assess the level of human development near wetlands. But there were no ecological data available for supporting the ecologically-effective part and the linkage between BAF and biodiversity was not known. The third part of this study was to test whether the BAF value correlated with species diversity of wetlands. I used ABC method to study crab composition of the four wetlands in central Taiwan and collected soil samples to analyze soil content. The adjacent land use in four wetlands was included to obtain BAF value and crab community was to obtain Shannon-Wiener index and Simpson indices. Results indicated that crab community was affected by soil organic content, soil water content, soil particle size, soil pH, and habitat types. From my study in 2013, the crab community in Gaomei wetland was under higher environmental stress than that in 2003, and the composition of crab communities in 2003 and 2013 were different. It could result from the human disturbance on Gaomei wetland. There was no correlation between BAF and biodiversity indices (Shannon-Wiener and Simpson indices), but crab community was significantly different in different types of adjacent land use. The environment of wetland may be affected by adjacent land use and crab community may be indirectly affected by adjacent land use.