Bibliografías temáticas / Molecular imaging applications

Literatura académica sobre el tema "Molecular imaging applications"

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Publicado: 11 de marzo de 2023

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Artículos de revistas sobre el tema "Molecular imaging applications"

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Ozeki, Yasuyuki. "Molecular vibrational imaging by stimulated Raman scattering microscopy: principles and applications [Invited]". Chinese Optics Letters 18, n.º 12 (2020): 121702. http://dx.doi.org/10.3788/col202018.121702.

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Tian, M. "Molecular Imaging: Fundamentals and Applications". Journal of Nuclear Medicine 56, n.º 2 (8 de enero de 2015): 329. http://dx.doi.org/10.2967/jnumed.114.153353.

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Li, King C. P., Sunil D. Pandit, Samira Guccione y Mark D. Bednarski. "Molecular Imaging Applications in Nanomedicine". Biomedical Microdevices 6, n.º 2 (junio de 2004): 113–16. http://dx.doi.org/10.1023/b:bmmd.0000031747.05317.81.

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Hildebrandt, Isabel Junie y Sanjiv Sam Gambhir. "Molecular imaging applications for immunology". Clinical Immunology 111, n.º 2 (mayo de 2004): 210–24. http://dx.doi.org/10.1016/j.clim.2003.12.018.

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Petrik, Milos, Chuangyan Zhai, Hubertus Haas y Clemens Decristoforo. "Siderophores for molecular imaging applications". Clinical and Translational Imaging 5, n.º 1 (11 de octubre de 2016): 15–27. http://dx.doi.org/10.1007/s40336-016-0211-x.

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Heneweer, Carola y Jan Grimm. "Clinical applications in molecular imaging". Pediatric Radiology 41, n.º 2 (3 de diciembre de 2010): 199–207. http://dx.doi.org/10.1007/s00247-010-1902-5.

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James, Michelle L. y Sanjiv S. Gambhir. "A Molecular Imaging Primer: Modalities, Imaging Agents, and Applications". Physiological Reviews 92, n.º 2 (abril de 2012): 897–965. http://dx.doi.org/10.1152/physrev.00049.2010.

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Molecular imaging is revolutionizing the way we study the inner workings of the human body, diagnose diseases, approach drug design, and assess therapies. The field as a whole is making possible the visualization of complex biochemical processes involved in normal physiology and disease states, in real time, in living cells, tissues, and intact subjects. In this review, we focus specifically on molecular imaging of intact living subjects. We provide a basic primer for those who are new to molecular imaging, and a resource for those involved in the field. We begin by describing classical molecular imaging techniques together with their key strengths and limitations, after which we introduce some of the latest emerging imaging modalities. We provide an overview of the main classes of molecular imaging agents (i.e., small molecules, peptides, aptamers, engineered proteins, and nanoparticles) and cite examples of how molecular imaging is being applied in oncology, neuroscience, cardiology, gene therapy, cell tracking, and theranostics (therapy combined with diagnostics). A step-by-step guide to answering biological and/or clinical questions using the tools of molecular imaging is also provided. We conclude by discussing the grand challenges of the field, its future directions, and enormous potential for further impacting how we approach research and medicine.
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Cai, Jiong, Zhaofei Liu, Fan Wang y Fang Li. "Phage Display Applications for Molecular Imaging". Current Pharmaceutical Biotechnology 11, n.º 6 (1 de septiembre de 2010): 603–9. http://dx.doi.org/10.2174/138920110792246573.

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Zhao, Ming, Xuefeng Wang, Gregory M. Lawrence, Patricio Espinoza y David D. Nolte. "Molecular Interferometric Imaging for Biosensor Applications". IEEE Journal of Selected Topics in Quantum Electronics 13, n.º 6 (2007): 1680–90. http://dx.doi.org/10.1109/jstqe.2007.911002.

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Mountz, John D., Hui-Chen Hsu, Qi Wu, Hong-Gang Liu, Huang-Ge Zhang y James M. Mountz. "Molecular imaging: New applications for biochemistry". Journal of Cellular Biochemistry 87, S39 (2002): 162–71. http://dx.doi.org/10.1002/jcb.10434.

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Tesis sobre el tema "Molecular imaging applications"

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GAMBINO, GIUSEPPE. "High-relaxivity systems and molecular imaging probes for Magnetic Resonance Imaging applications". Doctoral thesis, Università del Piemonte Orientale, 2014. http://hdl.handle.net/11579/46171.

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Chow, Mei-kwan April y 周美君. "Cellular, molecular and metabolic magnetic resonance imaging: techniques and applications". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44901148.

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Bottrill, Melanie Clare. "New routes to biocompatible nanoparticles and their applications in molecular imaging". Thesis, Imperial College London, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506114.

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Tang, Mei-yee y 鄧美宜. "Characterizations and applications of carbon nanotubes contrast agentsin magnetic resonance molecular imaging". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44701391.

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Ma, Yun. "Photofunctional molecular materials for chemical sensing, bioimaging and electrochromic applications". HKBU Institutional Repository, 2015. https://repository.hkbu.edu.hk/etd_oa/206.

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This thesis is dedicated to developing novel photofunctional molecular materials for the applications in chemical sensing, bioimaging and electrochromic. To begin with, a brief introduction of photofunctional molecular materials and an overview of their applications in chemical sensing, bioimaging and electrochromic were presented in Chapter 1. In chapter 2, we have synthesized a series of water-soluble phosphorescent cationic iridium(III) solvato complexes (1-7) as multicolor cellular probes for imaging in living cells. All of these complexes can be dissolved in PBS. The emission of complexes can be tuned from green to red by changing the chemical structure of cyclomedtalating ligands. All complexes exhibit low cytotoxicity to living cells and exhibit cell membrane permeability and specific staining of cytoplasm. They enter the cells by the mechanism of energy-independent passive diffusion mechanisms. More importantly, complex 7 can act as a two-photon phosphorescent cellular probe, and fluorescence lifetime imaging microscopy is successfully applied for bioimaging in the presence of short-lived background fluorescence. We developed two excellent optical probes for CO2 detection in Chapter 3. The first one for the CO2 detection is a phosphorescent probe based on an iridium(III) complex with 2-phenylimidazo-[4,5-f][1,10]phenanthroline. After bubbling CO2 into the detection solution, the quenched phosphorescence by the addition of CH3COO can be recovered. Photobleaching experiment demonstrates that this phosphorescent CO2 probe shows higher photostability than some of the reported organic probes. More importantly, the time-resolved PL experiment demonstrates that this probe can be used to detect CO2 in the presence of strong background fluorescence, which improves the sensitivity and signal-to-noise ratio of the sensor in complicated media. The second one is a water-soluble fluorescent probe based on tetraphenylethene derivative. After bubbling CO2 into the detection solution, remarkable color change and fluorescence enhancement could be observed. The response of this probe to CO2 in aqueous solution is fast and the detection limit is about 2.4 × 106 M. To emphasize the practical application of this probe, a porous film was successfully fabricated by mixing the dye with sodium carboxymethyl cellulose in water, which can serve as an efficient CO2 gas sensor. More importantly, this probe exhibits low cytotoxicity towards live cells and has the ability to monitor the external CO2 concentration changes of living cells. Chapter 4 focused on the development of novel soft salt based phosphorescent probe. This type of probe consists of two oppositely charged ionic complexes with two distinguishable emission colors, which makes it a perfect candidate as a ratiometric probe. The emission color of 10 changes from blue to red with increasing pH value. 10 is cell-permeable and exhibits low cytotoxicity, and it has been successfully applied for ratiometric pH imaging with the use of confocal microscopy, demonstrating its great potential for intracellular environment monitoring. Furthermore, phosphorescence lifetime imaging experiments can detect intracellular pH variations by photoluminescence lifetime measurements, which allowed for eliminating background fluorescence and selecting long-lived phosphorescence images. Quantitative measurement of intracellular pH fluctuations caused by oxidative stress has been successfully carried out for 10 based on the pH-dependent calibration curve. A series of cationic Zn(II) complexes has been designed and synthesized in chapter 5. The photophysical properties of these Zn(II) complexes are affected by the counterions. By altering the counterions, the emission peak can be changed from 549 nm to 622 nm. Interestingly, the CIE coordinate and the emission colors can be simply tuned by adjusting the concentration of 11d in the polyether. Under an electric field of about 15 V applied onto the electrodes, the emission color of the solution of 11b-11d near the cathode changed its original emission color to sky blue. Based on this interesting electrochromic fluorescence of 11d, a quasi-solid information recording device has been successfully designed. Furthermore, data encryption has been realized by combining 1d with BODIPY, and information decoding processed has been accomplished, for the first time, by employing TPA excitation techniques, in which the large TPA cross section of 11d is differentiated from small TPA cross section of common organic dyes. Finally, Chapters 6 and 7 present the concluding remarksand the experimental details of the work described in Chapters 25
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Favicchio, Rosy. "Fluorescence molecular tomography evaluation and applications for in vivo imaging of tumour proliferation". Thesis, University of Portsmouth, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.516877.

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Persson, Gustav. "Temporal Modulation in Fluorescence Spectroscopy and Imaging for Biological Applications". Doctoral thesis, KTH, Experimentell biomolekylär fysik, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-10243.

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This thesis explores the benefits of intensity modulation for the purpose of extending the range of applications of fluorescence spectroscopy and imaging in cellular and molecular biology and medicine. Long-lived transient states of fluorescent molecules can, because of their long lifetimes, be used to detect subtle changes in the microenvironment of the molecule. A method for determining the kinetic rates for transitions to and from such states by registration of changes in the average fluorescence intensity related to different modulation of the excitation source is introduced. It combines the detection sensitivity of fluorescence with the environmental sensitivity of the long-lived transient states and allows the use of slow detectors such as CCD cameras, making parallelization and wide-field imaging possible developments. An extension of this method, generating image contrast based on triplet state population using a standard laser scanning microscope, is also shown. A strategy to combine fluorescence correlation spectroscopy (FCS) with modulated excitation, in a way that allows extraction of correlation data for all correlation times, is presented. This enables the use of modulation to optimize measurement conditions with respect to photophysical properties of the dyes used. FCS with modulated excitation will probably prove useful in future studies involving multiple kinetic processes occurring in overlapping time ranges. One of the ideas from this project also constitutes a powerful method for generating artifact free correlation curves from data sets where sections have been removed. This is potentially very useful in biological studies where spikes in the measurements often cause problems. In the final project, cross-correlation and alternating excitation are combined in measurements on a pH-sensitive ratiometric dye to clearly distinguish the protonation–deprotonation dynamics from other processes. The presented approach makes the protonation related fluctuations manifest themselves as a very distinct anti-correlating component in the correlation curve. This enables robust data analysis using a simple model.
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Friedman, Mikaela. "Affibody molecules targeting the epidermal growth factor receptor for tumor imaging applications". Doctoral thesis, Stockholm : School of Biotechnology, Royal Institute of Technology (KTH), 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4710.

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Daryaei, Iman y Iman Daryaei. "Study, Evaluation, and Applications of MRI Contrast Agents that Work Based on CEST and T2-EX Mechanisms". Diss., The University of Arizona, 2017. http://hdl.handle.net/10150/625366.

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MRI is a powerful imaging method that offers several advantages including non-ionizing radiation, significant depth of penetration, and great spatial resolution. Current demand for precision medicine and the movement toward personalized medicine have encouraged researchers in the field of medical imaging to develop MRI-based techniques. Various techniques are now available for molecular imaging by MRI. MRI started by utilizing T1 relaxation properties of molecules but soon after other relaxation mechanisms such as T2 and recently Chemical Exchange Saturation Transfer (CEST) were developed. Each of those MRI techniques offers advantages and disadvantages such as differences in experimental procedures, complexity of the method, selectivity and specificity of signals, and translation into clinical applications. We have been developing MRI techniques and responsive contrast agents for CEST MRI in the Pagel laboratory (Contrast Agent and Molecular Imaging Laboratory, also called CAMEL) for the past decade. We have mainly utilized MRI techniques and responsive contrast agents to detect and measure cancer biomarkers. Detection of the activity of enzymes and measurement of pH have been our main focus, and we have developed catalyCEST MRI probes and techniques for the detection of the activity of enzymes and acidoCEST for the measurement of pH. My research started with investigation on paramagnetic agents as potential CEST MRI probes (paraCEST) and continued with an investigation on diamagnetic agents (diaCEST). I completed several projects in which I prepared and evaluated paraCEST and diaCEST contrast agents for the detection of DT-diaphorase, and alkaline phosphatase enzymes, respectively. Although CEST MRI was my main activity in CAMEL, I started a new direction in CAMEL after encountering a series of observations that were unexplainable with CEST MRI. Through my research, I introduced a new class of responsive contrast agents based on the T2-Exchange (T2-Ex) relaxation mechanism. I employed the T2-Ex mechanism to evaluate responsive contrast agents for the detection of nitric oxide biomolecule and nitroreductase enzyme. My research activities in the CAMEL group resulted in one review paper, one book chapter, two published research articles, and two submitted research manuscripts at the time of preparing my PhD dissertation. In addition to my projects, I was involved in another project that focused on nanocapsule drug delivery, which resulted in a second author publication.
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Wilson, Neil. "Studies on the reactivity of copper complexes with NO and CO, and their applications in molecular imaging". Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9490.

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This thesis is concerned with the development of metal complexes capable of selectively trapping and releasing nitric oxide and carbon monoxide. In the case of the former, we have utilised copper(II) complexes containing a nitrobenzofurazan (NBD) fluorophore which exhibit a restoration of fluorescence in the presence of nitric oxide. These complexes have shown to be water soluble, cell permeable, non toxic and selective towards nitric oxide over several other reactive oxygen and nitrogen species of biological relevance. For this reason these complexes have been used for the cellular imaging of nitric oxide. We have shown that our complexes can be localised within the cell membrane and can be used to image NO with a detection limit of 1μM. The second part of this thesis deals with the synthesis of copper(I) complexes of tris(2- pyridylmethyl)amine (tmpa) and their reactivity towards carbon monoxide. The aim of these studies was to develop pre-concentrating reagents capable of trapping 11CO for radiolabelling applications (more specifically for Positron Emission Tomography (PET) labelling). Indeed, these copper(I) complexes have been shown to trap near quantitative amounts of 11CO from nitrogen-rich gas streams without the need for elevated pressure or low temperature. To be useful, the trapping/release system must be compatible with some means of incorporating the CO into the desired target molecule. It has been demonstrated that [Cu(tmpa)(CO)]+ can be used as the CO source when performing palladium-catalysed carbonylations between amines and aryl halides to form amides.
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Libros sobre el tema "Molecular imaging applications"

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1953-, Rudin M., ed. Molecular imaging: Principles and applications in biomedical research. Hackensack, NJ: Imperial College Press, 2005.

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Acharya, Amitabha, ed. Nanomaterial - Based Biomedical Applications in Molecular Imaging, Diagnostics and Therapy. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-4280-0.

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1956-, Whitaker Benjamin J., ed. Imaging in molecular dynamics: Technology and applications (a user's guide). Cambridge: Cambridge University Press, 2003.

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H, Bach P. y European Workshop on Microscopic Imaging, Fluorescence, and Microinjection in Biotechnology (1st : 1992 : London, England), eds. Biotechnology applications of microinjection, microscopic imaging, and fluorescence. New York: Plenum Press, 1993.

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Srinivasan, Gokulakrishnan. Vibrational spectroscopic imaging for biomedical applications. New York: McGraw-Hill, 2010.

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Weaver, John B. y Robert C. Molthen. Medical imaging 2011: Biomedical applications in molecular, structural, and functional imaging : 13-16 February 2011, Lake Buena Vista, United States. Editado por SPIE (Society), Dynasil Corporation RMD Research y American Physiological Society (1887- ). Bellingham, Wash: SPIE, 2011.

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Weaver, John B. y Robert C. Molthen. Medical imaging 2012: Biomedical applications in molecular, structural, and functional imaging : 5-7 February 2012, San Diego, California, United States. Editado por SPIE (Society), Agilent Technologies y American Association of Physicists in Medicine. Bellingham, Wash: SPIE, 2012.

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P, Savitsky Alexander, Wachter Rebekka M y Society of Photo-optical Instrumentation Engineers., eds. Genetically engineered probes for biomedical applications: 24 January 2006, San Jose, California, USA. Bellingham, Wash: SPIE, 2006.

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Achilefu, Samuel. Molecular probes for biomedical applications II: 21-22 January 2008, San Jose, California, USA. Editado por SPIE (Society). Bellingham, Wash: SPIE, 2008.

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Achilefu, Samuel. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications: 26-29 January 2009, San Jose, California, United States. Bellingham, Wash: SPIE, 2009.

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Capítulos de libros sobre el tema "Molecular imaging applications"

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Kundra, Vikas. "INTRODUCTION TO MOLECULAR IMAGING". En Drug Delivery Applications of Noninvasive Imaging, 1–11. Hoboken, NJ: John Wiley & Sons, Inc, 2013. http://dx.doi.org/10.1002/9781118356845.ch1.

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Nanni, Cristina y Stefano Fanti. "Applications of Small Animal PET". En Molecular Imaging in Oncology, 247–55. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-10853-2_8.

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Schelhaas, Sonja. "Applications of Small Animal PET". En Molecular Imaging in Oncology, 493–507. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-42618-7_14.

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Gao, Xiaohu y Shivang R. Dave. "Quantum Dots for Cancer Molecular Imaging". En Bio-Applications of Nanoparticles, 57–73. New York, NY: Springer New York, 2007. http://dx.doi.org/10.1007/978-0-387-76713-0_5.

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Jun, Young-wook, Jung-tak Jang y Jinwoo Cheon. "Magnetic Nanoparticle Assisted Molecular MR Imaging". En Bio-Applications of Nanoparticles, 85–106. New York, NY: Springer New York, 2007. http://dx.doi.org/10.1007/978-0-387-76713-0_7.

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Li, Dengfeng, Yesi Shi y Gang Liu. "Aptamer-Based Probes for Molecular Imaging". En Aptamers for Medical Applications, 31–52. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-33-4838-7_2.

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Jackson, E. F. "Magnetic Resonance Spectroscopy: Physical Principles and Applications". En Molecular Imaging in Oncology, 47–70. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59879-1_4.

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Ritt, Philipp y Torsten Kuwert. "Quantitative SPECT/CT—Technique and Clinical Applications". En Molecular Imaging in Oncology, 565–90. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-42618-7_17.

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Cai, Weibo, Ting Gao y Hao Hong. "Biomedical Applications of Single-Walled Carbon Nanotubes". En Nanoplatform-Based Molecular Imaging, 481–528. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9780470767047.ch20.

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Diagaradjane, Parmeswaran, Pranshu Mohindra y Sunil Krishnan. "Theranostic Applications of Gold Nanoparticles in Cancer". En Nanoplatform-Based Molecular Imaging, 639–57. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9780470767047.ch26.

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Actas de conferencias sobre el tema "Molecular imaging applications"

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Smith, Jason T., Enagnon Aguénounon, Sylvain Gioux y Xavier Intes. "Depth-resolved macroscopic fluorescence lifetime imaging improved though spatial frequency domain imaging". En Molecular-Guided Surgery: Molecules, Devices, and Applications VII, editado por Summer L. Gibbs, Brian W. Pogue y Sylvain Gioux. SPIE, 2021. http://dx.doi.org/10.1117/12.2578495.

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Feroldi, Fabio, Margherita Vaselli, Mariska Verlaan, Helene Knaus, Valentina Davidoiu, Danielle Vugts, Carla Molthoff, Guus van Dongen y Johannes F. de Boer. "Combined structural and molecular imaging using optical coherence tomography and immunofluorescence imaging (Conference Presentation)". En Molecular-Guided Surgery: Molecules, Devices, and Applications VI, editado por Summer L. Gibbs, Brian W. Pogue y Sylvain Gioux. SPIE, 2020. http://dx.doi.org/10.1117/12.2545222.

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Harris, T. D., J. J. Macklin, J. K. Trautman y L. E. Brus. "Imaging and Time-Resolved Spectroscopy of Single Molecules". En Laser Applications to Chemical and Environmental Analysis. Washington, D.C.: Optica Publishing Group, 1996. http://dx.doi.org/10.1364/lacea.1996.lwd.5.

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Recent progress in the fluorescence detection of individual molecules [1-8] suggests that a single dye molecule can be a useful tool to probe chemical identity and activity. Measurement of fluorescence lifetime [5,6] and spectrum [6] can be augmented by knowledge of molecular orientation using polarized light [3], and triplet [2] and photoisomer excitation, as well as diffusion processes, via fluorescence-intensity correlation. Applications of fluorescent probes include the study of the dynamic conformation of membrane-bound proteins, transport of and signaling by messenger molecules, and the optical detection of the sequence of DNA. While molecules can be spatially located using near-field microscopy [5-8], near-field probes can perturb the molecule under study. We show here that molecular properties can be determined easily and in a non-perturbative manner using far-field illumination, and we obtain unperturbed spectral and lifetime data that cannot be extracted from an ensemble measurement.
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Angelo, Joseph P., Martijn van de Giessen y Sylvain Gioux. "Real-time endoscopic oxygenation imaging using single snapshot of optical properties (SSOP) imaging (Conference Presentation)". En Molecular-Guided Surgery: Molecules, Devices, and Applications II, editado por Brian W. Pogue y Sylvain Gioux. SPIE, 2016. http://dx.doi.org/10.1117/12.2213117.

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Wax, Adam. "Molecular Contrast in Interferometric Imaging". En CLEO: Applications and Technology. Washington, D.C.: OSA, 2015. http://dx.doi.org/10.1364/cleo_at.2015.aw4h.1.

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Chen, Yu. "Multi-Scale Optical Molecular Imaging". En CLEO: Applications and Technology. Washington, D.C.: OSA, 2015. http://dx.doi.org/10.1364/cleo_at.2015.aw4h.2.

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Tang, Yue, Jessica M. Sin, I. Leah Gitajn, Xu Cao, Xinyue Han, Jonathan T. Elliott, Xiaohan Yu et al. "Dynamic contrast-enhanced fluorescence imaging compared with MR imaging in evaluating bone perfusion during open orthopedic surgery". En Molecular-Guided Surgery: Molecules, Devices, and Applications VIII, editado por Summer L. Gibbs, Brian W. Pogue y Sylvain Gioux. SPIE, 2022. http://dx.doi.org/10.1117/12.2608382.

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Yang, Bin y James W. Tunnell. "Attenuation correction in molecular fluorescence imaging (Conference Presentation)". En Molecular-Guided Surgery: Molecules, Devices, and Applications II, editado por Brian W. Pogue y Sylvain Gioux. SPIE, 2016. http://dx.doi.org/10.1117/12.2211155.

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Choi, Hak Soo. "Nanoprobes for optical fluorescence imaging (Conference Presentation)". En Molecular-Guided Surgery: Molecules, Devices, and Applications IV, editado por Greg Biggs, Brian W. Pogue y Sylvain Gioux. SPIE, 2018. http://dx.doi.org/10.1117/12.2287971.

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Bogyo, Matthew. "Imaging cancer with protease activated optical probes". En Molecular-Guided Surgery: Molecules, Devices, and Applications VIII, editado por Summer L. Gibbs, Brian W. Pogue y Sylvain Gioux. SPIE, 2022. http://dx.doi.org/10.1117/12.2617795.

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Informes sobre el tema "Molecular imaging applications"

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Camden, Jon P. Plasmon Mapping in Metallic Nanostructures and its Application to Single Molecule Surface Enhanced Raman Scattering: Imaging Electromagnetic Hot-Spots and Analyte Location. Office of Scientific and Technical Information (OSTI), julio de 2013. http://dx.doi.org/10.2172/1087663.

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Jun, Ji Hyun. Development of high-spatial and high-mass resolution mass spectrometric imaging (MSI) and its application to the study of small metabolites and endogenous molecules of plants. Office of Scientific and Technical Information (OSTI), enero de 2012. http://dx.doi.org/10.2172/1048509.

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Shani, Uri, Lynn Dudley, Alon Ben-Gal, Menachem Moshelion y Yajun Wu. Root Conductance, Root-soil Interface Water Potential, Water and Ion Channel Function, and Tissue Expression Profile as Affected by Environmental Conditions. United States Department of Agriculture, octubre de 2007. http://dx.doi.org/10.32747/2007.7592119.bard.

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Constraints on water resources and the environment necessitate more efficient use of water. The key to efficient management is an understanding of the physical and physiological processes occurring in the soil-root hydraulic continuum.While both soil and plant leaf water potentials are well understood, modeled and measured, the root-soil interface where actual uptake processes occur has not been sufficiently studied. The water potential at the root-soil interface (yᵣₒₒₜ), determined by environmental conditions and by soil and plant hydraulic properties, serves as a boundary value in soil and plant uptake equations. In this work, we propose to 1) refine and implement a method for measuring yᵣₒₒₜ; 2) measure yᵣₒₒₜ, water uptake and root hydraulic conductivity for wild type tomato and Arabidopsis under varied q, K⁺, Na⁺ and Cl⁻ levels in the root zone; 3) verify the role of MIPs and ion channels response to q, K⁺ and Na⁺ levels in Arabidopsis and tomato; 4) study the relationships between yᵣₒₒₜ and root hydraulic conductivity for various crops representing important botanical and agricultural species, under conditions of varying soil types, water contents and salinity; and 5) integrate the above to water uptake term(s) to be implemented in models. We have made significant progress toward establishing the efficacy of the emittensiometer and on the molecular biology studies. We have added an additional method for measuring ψᵣₒₒₜ. High-frequency water application through the water source while the plant emerges and becomes established encourages roots to develop towards and into the water source itself. The yᵣₒₒₜ and yₛₒᵢₗ values reflected wetting and drying processes in the rhizosphere and in the bulk soil. Thus, yᵣₒₒₜ can be manipulated by changing irrigation level and frequency. An important and surprising finding resulting from the current research is the obtained yᵣₒₒₜ value. The yᵣₒₒₜ measured using the three different methods: emittensiometer, micro-tensiometer and MRI imaging in both sunflower, tomato and corn plants fell in the same range and were higher by one to three orders of magnitude from the values of -600 to -15,000 cm suggested in the literature. We have added additional information on the regulation of aquaporins and transporters at the transcript and protein levels, particularly under stress. Our preliminary results show that overexpression of one aquaporin gene in tomato dramatically increases its transpiration level (unpublished results). Based on this information, we started screening mutants for other aquaporin genes. During the feasibility testing year, we identified homozygous mutants for eight aquaporin genes, including six mutants for five of the PIP2 genes. Including the homozygous mutants directly available at the ABRC seed stock center, we now have mutants for 11 of the 19 aquaporin genes of interest. Currently, we are screening mutants for other aquaporin genes and ion transporter genes. Understanding plant water uptake under stress is essential for the further advancement of molecular plant stress tolerance work as well as for efficient use of water in agriculture. Virtually all of Israel’s agriculture and about 40% of US agriculture is made possible by irrigation. Both countries face increasing risk of water shortages as urban requirements grow. Both countries will have to find methods of protecting the soil resource while conserving water resources—goals that appear to be in direct conflict. The climate-plant-soil-water system is nonlinear with many feedback mechanisms. Conceptual plant uptake and growth models and mechanism-based computer-simulation models will be valuable tools in developing irrigation regimes and methods that maximize the efficiency of agricultural water. This proposal will contribute to the development of these models by providing critical information on water extraction by the plant that will result in improved predictions of both water requirements and crop yields. Plant water use and plant response to environmental conditions cannot possibly be understood by using the tools and language of a single scientific discipline. This proposal links the disciplines of soil physics and soil physical chemistry with plant physiology and molecular biology in order to correctly treat and understand the soil-plant interface in terms of integrated comprehension. Results from the project will contribute to a mechanistic understanding of the SPAC and will inspire continued multidisciplinary research.
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Validation of Multispectral Imaging (MSI) technology for food and feed analysis. Food Standards Agency, agosto de 2021. http://dx.doi.org/10.46756/sci.fsa.zcr161.

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The current testing environment for food and feed samples is complex and uses a wide variety of analytical technologies that range from chemical to PCR-based. Whilst these approaches can be deemed fit for purpose in terms of their final analytical result, they tend to be highly specialised and require considerable frontend processing to ensure that the target analyte can be reliably detected and quantified. These combined sample processing and analytical requirements typically impact on testing times and have associated cost implications that must be factored into routine testing and monitoring applications. MSI uses multiple discrete and informative wavelengths covering regions such as the UV and near infra-red spectrum to quickly determine surface colour, texture and possible chemical composition. Compared to traditional molecular biology approaches utilising DNA extraction followed by PCR-based analyses, MSI can simplify and reduce the time/costs associated with sample analysis. It is rapid and non-destructive.
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