Bibliografías temáticas / Molecular aspects

Literatura académica sobre el tema "Molecular aspects"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 6 de septiembre de 2023

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Artículos de revistas sobre el tema "Molecular aspects"

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Fountoulakis, Stelios y Agathocles Tsatsoulis. "Molecular genetic aspects and pathophysiology of endocrine hypertension". HORMONES 5, n.º 2 (15 de abril de 2006): 90–106. http://dx.doi.org/10.14310/horm.2002.11173.

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Trojanowska, Maria. "Molecular aspects of scleroderma". Frontiers in Bioscience 7, n.º 1-3 (2002): d608. http://dx.doi.org/10.2741/trojan.

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Cazzola, Mario y Maria Gabriella Matera. "Molecular aspects of asthma". Molecular Aspects of Medicine 85 (junio de 2022): 101087. http://dx.doi.org/10.1016/j.mam.2022.101087.

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Wood, Richard D. y Steven G. Sedgwick. "Molecular aspects of mutagenesis". Mutagenesis 1, n.º 6 (1986): 399–405. http://dx.doi.org/10.1093/mutage/1.6.399.

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Hashimoto, T. y Y. Yamada. "Alkaloid Biogenesis: Molecular Aspects". Annual Review of Plant Physiology and Plant Molecular Biology 45, n.º 1 (junio de 1994): 257–85. http://dx.doi.org/10.1146/annurev.pp.45.060194.001353.

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Andreoli, Thomas E. "Molecular Aspects of Endocrinology". Hospital Practice 24, n.º 8 (15 de agosto de 1989): 11–12. http://dx.doi.org/10.1080/21548331.1989.11703755.

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Lau, T. M., B. Affandi y P. A. W. Rogers. "Molecular aspects of implantation". MHR: Basic science of reproductive medicine 2, n.º 4 (abril de 1996): 233–37. http://dx.doi.org/10.1093/molehr/2.4.233.

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Simón, Carlos, Maria Jose Gimeno, Amparo Mercader, Ana Francés, Juan Garcia Velasco, Jose Remohi, Mary Lake Polan y Antonio Pellicer. "Molecular aspects of implantation". Molecular Human Reproduction 2, n.º 6 (1996): 405–24. http://dx.doi.org/10.1093/molehr/2.6.405.

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Lopata, Alexander. "Molecular aspects of implantation". Molecular Human Reproduction 2, n.º 7 (1996): 519–25. http://dx.doi.org/10.1093/molehr/2.7.519.

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Aplin, John D., Catherine Spanswick, Farhad Behzad, Susan J. Kimber y Ljiljana Vićovac. "Molecular aspects of implantation". Molecular Human Reproduction 2, n.º 7 (1996): 527–34. http://dx.doi.org/10.1093/molehr/2.7.527.

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Tesis sobre el tema "Molecular aspects"

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Shellcock, Carole. "Molecular aspects of algal biofuels". Thesis, University of Aberdeen, 2013. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=217886.

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The ability of micro–algae to respond to diverse and often rapidly changing habitats has been attributed to the versatility of their cellular lipids. Amongst these the energy rich triacylglycerols (TAG) have attracted considerable attention due to their potential use as feedstock for renewable biofuel. Although micro-algae have considerable advantages over other biofuel sources there are constraints to their utilisation. Improvements are required in certain areas including efficiencies in production and enhanced lipid yields if micro-algal biofuels are to become commercially feasible. To achieve this, genetic and metabolic manipulation will be essential and therefore a greater understanding of the lipid biosynthetic pathways is required. In this study the expression of genes putatively involved in TAG biosynthesis in the diatom Phaeodactylum tricornutum was investigated, for the first time, with CO2 supplementation and low pH stress over the entire growth cycle. This molecular analysis was combined with physiochemical examination of the lipid accumulation in the micro-algal model. The results indicated that TAG accumulation was enhanced by CO2 supplementation and occurred predominantly during the stationary growth phase. The molecular analysis revealed increased expression for three genes of interest, encoding enzymes involved in the acyl dependent pathway: Glycerol-3-phosphate acyl transferase (GPAT) -7198728 (Phatrdraft_50031), lysophosphatidic acyl transferase (LPAAT) -7196550 (Phatrdraft_42446) and phosphotidic acid phosphatase (PAP) -7195747 (Phatrdraft_40261) in cultures supplemented with CO2. Under the same conditions up-regulation of a gene involved in the first committed step of fatty acid biosynthesis, Acetyl CoA carboxylase (ACC) -7194806 (Phatrdraft_54926) was also observed. Overall, this study provides further insight on the specific genes linked with increased TAG production in P. tricornutum and the identification of references genes suitable for normalisation of qPCR data across the growth cycle and under CO2 supplementation, thus providing the tools needed for future molecular studies of P. tricornutum lipid production.
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VanEngelen, Michael Robert. "Molecular aspects of uranium toxicity". Thesis, Montana State University, 2009. http://etd.lib.montana.edu/etd/2009/vanengelen/VanEngelenM1209.pdf.

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Rakowski, Andrew George. "Molecular aspects of viroid activities /". Title page, contents and summary only, 1992. http://web4.library.adelaide.edu.au/theses/09PH/09phr162.pdf.

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Chauthaiwale, V. M. "Molecular aspects of chainia sp". Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1992. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3005.

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Gonçalves, Luiz Ricardo. "Detecção e caracterização molecular de espécies de Mycoplasma e Bartonella em roedores silvestres e sinantrópicos no Brasil /". Jaboticabal, 2016. http://hdl.handle.net/11449/141958.

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Orientador: Marcos Rogério André
Coorientador: Rosangela Zacarias Machado
Banca: Paulo Eduardo Neves Ferreira Velho
Banca: José Maurício Barbanti Duarte
Resumo: Patógenos transmitidos por vetores artrópodes são mundialmente importantes, podendo causar enfermidades tanto no homen quanto nos animais. Recentemente, diversos estudos têm sido realizados a fim de elucidar o papel dos animais selvagens na epidemiologia desses patógenos. A identificação desses microrganismos, assim como dos seus vetores e hospedeiros, permite mapear áreas de ocorrência desses patógenos, auxiliando os órgãos competentes na elaboração de medidas de controle. Sendo assim, o presente estudo teve como objetivo detectar e caracterizar, por métodos moleculares, micoplasmas hemotróficos e bartonelas em amostras de baço de roedores selvagens e sinantrópicos distribuídos em cinco biomas brasileiros. Para tal, foram analisadas 500 amostras de roedores pertencentes a 51 espécies distribuídas em 13 estados. Dentre as 457 amostras de DNA positivas nos ensaios de PCR baseados nos genes Interphotoreceptor retinoid binding protein [IRBP] ou Glyceraldehyde-3-phosphate dehydrogenase [GAPDH], 100 (21,9%) mostraram-se positivas para Mycoplasma spp. por meio da cPCR baseada no gene 16S rRNA. A análise filogenética de 24 sequências do gene 16S rRNA mostrou a presença de dez diferentes clusters, todos agrupados dentro do grupo Mycoplasma haemofelis. O posicionamento filogenético associado com a baixa porcentagem de identidade entre as sequências amplificadas no presente estudo e aquelas previamente depositadas no Genbank sugere a circulação de novas espécies de hemoplasmas em roedo... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Arthropod-borne pathogens are wordwide important, causing diseases in humans and animals. Recently, several studies have been performed in order to elucidate the role of wild animals in the epidemiology of these pathogens. The identification of these microorganisms, as well as their vectors and hosts, allow to map the areas of occurrence of these pathogens, helping competent agencies in the development of control measures. Therefore, the present study aimed to detect and characterize, using molecular methods, hemotrophic mycoplasmas and Bartonella in wild and sinantropic rodent spleen samples distributed in five Brazilian biomes. For this purpose, 500 rodent samples belonging to 51 species distributed in 13 states were analyzed. Among 457 DNA samples positive to PCR assays based on the Interphotoreceptor retinoid binding protein [IRBP] or Glyceraldehyde-3-phosphate dehydrogenase [GAPDH]) genes, 100 (21.9%) were positive to Mycoplasma spp. by cPCR based on 16S rRNA gene. The phylogenetic analysis of 24 sequences of the 16S rRNA gene showed the presence of ten different clusters, which grouped within the Mycoplasma haemofelis group. The phylogenetic position associated with the low percentage of identity between the sequences amplified in the present study and those previously deposited in Genbank suggest the circulation of new hemoplasmas species in rodents in Brazil. Additionally, 117 (25.6%) samples were positive to Bartonella sp. by qPCR. The diversity analysis of the gltA ... (Complete abstract click electronic access below)
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Sörgjerd, Karin. "Molecular Aspects of Transthyretin Amyloid Disease". Doctoral thesis, Linköpings universitet, Biokemi, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-12566.

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This thesis was made to get a deeper understanding of how chaperones interact with unstable, aggregation prone, misfolded proteins involved in human disease. Over the last two decades, there has been much focus on misfolding diseases within the fields of biochemistry and molecular biotechnology research. It has become obvious that proteins that misfold (as a consequence of a mutation or outer factors), are the cause of many diseases. Molecular chaperones are proteins that have been defined as agents that help other proteins to fold correctly and to prevent aggregation. Their role in the misfolding disease process has been the subject for this thesis. Transthyretin (TTR) is a protein found in human plasma and in cerebrospinal fluid. It works as a transport protein, transporting thyroxin and holo-retinol binding protein. The structure of TTR consists of four identical subunits connected through hydrogen bonds and hydrophobic interactions. Over 100 point mutations in the TTR gene are associated with amyloidosis often involving peripheral neurodegeneration (familial amyloidotic polyneuropathy (FAP)). Amyloidosis represents a group of diseases leading to extra cellular deposition of fibrillar protein known as amyloid. We used human SH-SY5Y neuroblastoma cells as a model for neurodegeneration. Various conformers of TTR were incubated with the cells for different amounts of time. The experiments showed that early prefibrillar oligomers of TTR induced apoptosis when neuroblastoma cells were exposed to these species whereas mature fibrils were not cytotoxic. We also found increased expression of the molecular chaperone BiP in cells challenged with TTR oligomers. Point mutations destabilize TTR and result in monomers that are unstable and prone to aggregate. TTR D18G is naturally occurring and the most destabilized TTR mutant found to date. It leads to central nervous system (CNS) amyloidosis. The CNS phenotype is rare for TTR amyloid disease. Most proteins associated with amyloid disease are secreted proteins and secreted proteins must pass the quality control check within the endoplasmic reticulum (ER). BiP is a Hsp70 molecular chaperone situated in the ER. BiP is one of the most important components of the quality control system in the cell. We have used TTR D18G as a model for understanding how an extremely aggregation prone protein is handled by BiP. We have shown that BiP can selectively capture TTR D18G during co-expression in both E. coli and during over expression in human 293T cells and collects the mutant in oligomeric states. We have also shown that degradation of TTR D18G in human 293T cells occurs slower in presence of BiP, that BiP is present in amyloid deposition in human brain and mitigates cytotoxicity of TTR D18G oligomers.
Denna avhandling handlar om proteiner. Särskilt de som inte fungerar som de ska utan har blivit vad man kallar ”felveckade”. Anledningen till att proteiner veckas fel beror ofta (men inte alltid) på mutationer i arvsmassan. Felveckade proteiner kan leda till sjukdomar hos människor och djur (man brukar tala om amyloidsjukdomar), ofta av neurologisk karaktär. Exempel på amyloidsjukdomar är polyneuropati, där perifera nervsystemet är drabbat, vilket leder till begränsad rörelseförmåga och senare till förlamning; och Alzheimer´s sjukdom, där centrala nervsystemet är drabbat och leder till begränsad tankeförmåga och minnesförluster. Studierna som presenteras i denna avhandling har gått ut på att få en bättre förståelse för hur felveckade proteiner interagerar med det som vi har naturligt i cellerna och som fungerar som skyddande, hjälpande proteiner, så kallade chaperoner. Transtyretin (TTR) är ett protein som cirkulerar i blodet och transporterar tyroxin (som är ett hormon som bland annat har betydelse för ämnesomsättningen) samt retinol-bindande protein (vitamin A). I TTR genen har man funnit över 100 punktmutationer, vilka har kopplats samman med amyloidsjukdomar, bland annat ”Skellefteåsjukan”. Mutationer i TTR genen leder ofta till att proteinet blir instabilt vilket leder till upplösning av TTR tetrameren till monomerer. Dessa monomerer kan därefter sammanfogas på nytt men denna gång på ett sätt som är farligt för organismen. I denna avhandling har fokus legat på en mutation som kallas TTR D18G, vilken har identifierats i olika delar av världen och leder till en dödlig form av amyloidos i centrala nervsystemet. Det chaperon som vi har studerat benämns BiP och är beläget i en cellkomponent som kallas för det endoplasmatiska retiklet (ER). I ER finns cellens kontrollsystem i vilket det ses till att felveckade proteiner inte släpps ut utan istället bryts ned. Denna avhandling har visat att BiP kan fånga upp TTR D18G inuti celler och där samla mutanten i lösliga partiklar som i detta fall är ofarliga för cellen. Avhandligen har också visat att nedbrytningen av TTR D18G sker mycket långsammare när BiP finns i riklig mängd.
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Broomand, Amir. "Molecular aspects on voltage-sensor movement". Doctoral thesis, Linköping : Univ, 2007. http://www.bibl.liu.se/liupubl/disp/disp2007/med1028s.pdf.

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Njålsson, Runa Viđarr. "Molecular aspects of glutathione synthetase deficiency /". Stockholm, 2004. http://diss.kib.ki.se/2003/91-7349-808-4.

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Fawcett, Jonathan. "Molecular aspects of angiogenesis and metastasis". Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386753.

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Watkins, Craig Allen. "Molecular aspects of punta toro virus". Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239272.

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Libros sobre el tema "Molecular aspects"

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1941-, Hall Brian Keith y Newman Stuart, eds. Cartilage: Molecular aspects. Boca Raton, Fla: CRC Press, 1991.

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Epstein, O. Molecular aspects of primary bilaiary cirrhosis: Molecular aspects of medecine. Oxford: Pergamon, 1986.

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Shugar, David, Wojciech Rode y Edward Borowski, eds. Molecular Aspects of Chemotherapy. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-662-02740-0.

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Sies, Helmut, Leopold Flohé y Guido Zimmer, eds. Molecular Aspects of Inflammation. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76412-7.

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Aloni, Yosef, ed. Molecular Aspects of Papovaviruses. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-2087-6.

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Montalcini, Rita Levi, Pietro Calissano, Eric Richard Kandel y Adriana Maggi, eds. Molecular Aspects of Neurobiology. Berlin, Heidelberg: Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-70690-5.

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Jonoska, Nataša, Gheorghe Păun y Grzegorz Rozenberg, eds. Aspects of Molecular Computing. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/b94864.

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Rojas, Mauricio, Silke Meiners y Claude Jourdan Le Saux, eds. Molecular Aspects of Aging. Hoboken, NJ: John Wiley & Sons, Inc, 2014. http://dx.doi.org/10.1002/9781118396292.

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Molecular aspects of spermatogenesis. Austin: R.G. Landes Co., 1994.

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Hitoshi, Endou, ed. Molecular aspects of ammoniagenesis. Basel: Karger, 1991.

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Capítulos de libros sobre el tema "Molecular aspects"

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Kinloch, A. J. y R. J. Young. "Molecular Aspects". En Fracture Behaviour of Polymers, 43–73. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-017-1594-2_2.

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Öztürk, Münir, Volkan Altay, Khalid Rehman Hakeem y Eren Akçiçek. "Molecular Aspects". En Liquorice, 41–43. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-74240-3_6.

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Pandith, Shahzad A. y Mohd Ishfaq Khan. "Molecular Aspects". En Genus Rheum (Polygonaceae), 107–34. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9780429340390-6.

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Chen, Hungwen y Mei-Leng Cheong. "Syncytins: Molecular Aspects". En Cell Fusions, 117–37. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9772-9_5.

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Srinivasan, Sundaram y Raghavan Seshan. "Kinetic Aspects". En SpringerBriefs in Molecular Science, 47–55. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-20817-1_4.

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Shichi, Hitoshi. "Visual Phototransduction: Biochemical Aspects". En Molecular Electronics, 165–71. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4615-7482-8_19.

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Roche, Helen M., Herman E. Popeijus y Ronald P. Mensink. "Molecular Aspects of Nutrition". En Nutrition and Metabolism, 7–34. Oxford, UK: Wiley-Blackwell, 2011. http://dx.doi.org/10.1002/9781444327779.ch2.

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Conchon, S. y E. Clauser. "AT1 Receptor Molecular Aspects". En Angiotensin Vol. I, 269–95. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18495-6_12.

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Parke, D. V. "Molecular Aspects of Toxicology". En The Future of Predictive Safety Evaluation, 3–38. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3201-2_1.

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Anholt, Robert R. H. "Molecular Aspects of Olfaction". En Science of Olfaction, 51–79. New York, NY: Springer New York, 1992. http://dx.doi.org/10.1007/978-1-4612-2836-3_3.

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Actas de conferencias sobre el tema "Molecular aspects"

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Fernández, F., P. G. Ortega y D. R. Entem. "Molecular aspects of charm physics". En XII HADRON PHYSICS. AIP, 2013. http://dx.doi.org/10.1063/1.4795960.

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Yusof, Yuhani, Wen Li Lim, T. Elizabeth Goode, Nor Haniza Sarmin, Fong Wan Heng y Mohd Firdaus Abd Wahab. "Molecular aspects of DNA splicing system". En INTERNATIONAL CONFERENCE ON MATHEMATICS, ENGINEERING AND INDUSTRIAL APPLICATIONS 2014 (ICoMEIA 2014). AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4915678.

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Stefanovic, Darko, Milan Stojanovic, Mark Olah y Oleg Semenov. "Catalytic Molecular Walkers: Aspects of Product Release". En European Conference on Artificial Life 2013. MIT Press, 2013. http://dx.doi.org/10.7551/978-0-262-31709-2-ch172.

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Thomas, Nancy E. "Abstract CN12-05: Molecular aspects of melanoma". En Abstracts: AACR International Conference on Frontiers in Cancer Prevention Research‐‐ Dec 6–9, 2009; Houston, TX. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1940-6207.prev-09-cn12-05.

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Cherdyntseva, N., N. Litviakov, F. Ivanova, E. Denisov, P. Gervas y E. Cherdyntsev. "The molecular aspects of personalized anticancer treatment". En PHYSICS OF CANCER: INTERDISCIPLINARY PROBLEMS AND CLINICAL APPLICATIONS (PC’16): Proceedings of the International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications 2016. Author(s), 2016. http://dx.doi.org/10.1063/1.4960229.

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Ismail, Nurul Izzaty, Wan Heng Fong y Nor Haniza Sarmin. "Molecular aspects on generalisations of splicing languages". En PROCEEDINGS OF THE 27TH NATIONAL SYMPOSIUM ON MATHEMATICAL SCIENCES (SKSM27). AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0018377.

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Hartwig, Beppo y Martin Suhm. "CHIRALITY ASPECTS IN THE DIMERIZATION OF VICINAL DIOLS". En 74th International Symposium on Molecular Spectroscopy. Urbana, Illinois: University of Illinois at Urbana-Champaign, 2019. http://dx.doi.org/10.15278/isms.2019.mh05.

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BEZZINA, CONNIE R. y ARTHUR A. M. WILDE. "MOLECULAR, GENETIC AND CLINICAL ASPECTS OF ARRHYTHMIA DISORDERS". En Proceedings of the 31st International Congress on Electrocardiology. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812702234_0080.

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Kolubako, A. V. y Y. A. Nikolaichik. "Molecular aspects of Pectobacterium carotovorum – Solanum tuberosum interaction". En 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.124.

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Iskhakova, A. G., A. N. Toropovsky, A. V. Zolotarev, O. N. Pavlova y M. V. Komarova. "Molecular and Genetic Aspects of Diabetic Retinopathy Diagnosis". En Conference on Health and Wellbeing in Modern Society (CHW 2021). Paris, France: Atlantis Press, 2022. http://dx.doi.org/10.2991/ahsr.k.220103.053.

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Informes sobre el tema "Molecular aspects"

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Siedow, J. N. Molecular studies of functional aspects of plant mitochondria. Office of Scientific and Technical Information (OSTI), marzo de 1992. http://dx.doi.org/10.2172/7194216.

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Paul W. Bohn. Molecular Aspects of Transport in Thin Films of Controlled Architecture. Office of Scientific and Technical Information (OSTI), abril de 2009. http://dx.doi.org/10.2172/951203.

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Siedow, J. N. Molecular studies of functional aspects of plant mitochondria. Progress report. Office of Scientific and Technical Information (OSTI), marzo de 1992. http://dx.doi.org/10.2172/10184136.

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Hoffman, Eric P. Molecular Aspects of Muscle Damage and Denervation with Public Access Tools. Fort Belvoir, VA: Defense Technical Information Center, diciembre de 2004. http://dx.doi.org/10.21236/ada455552.

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Wackett, Lawrence, Raphi Mandelbaum y Michael Sadowsky. Bacterial Mineralization of Atrazine as a Model for Herbicide Biodegradation: Molecular and Applied Aspects. United States Department of Agriculture, enero de 1999. http://dx.doi.org/10.32747/1999.7695835.bard.

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Atrazine is a broadly used herbicide in agriculture and it was used here as a model to study the biodegradation of herbicides. The bacterium Pseudomonas sp. ADP metabolizes atrazine to carbon dioxide and ammonia and chloride. The genes encoding atrazine catabolism to cyanuric acid were cloned and expressed in Escherichia coli. The genes were designated atzA, atzB and atzC. Each gene was sequenced. The enzyme activities were characterized. AtzA is atrazine chlorohydrolase which takes atrazine to hydroxyatrizine. AtzB is hydroxyatrazine N-ethylaminohydrolase which produces N-isopropylammelide and N-ethylamine. AtzC is N-isopropylammelide N-isopropylaminohydrolase which produces cyanuric acid and N-isopropylamine. Each product was isolated and characterized to confirm their identity by chromatography and mass spectrometry. Sequence analysis indicated that each of the hydrolytic enzymes AtzA, AtzB and AtzC share identity which the aminohydrolase protein superfamily. Atrazine chlorohydrolase was purified to homogeneity. It was shown to have a kcat of 11 s-1 and a KM of 150 uM. It was shown to require a metal ion, either Fe(II), Mn(II) or Co(II), for activity. The atzA, atzB and atzC genes were shown to reside on a broad-host range plasmid in Pseudomonas sp. ADP. Six other recently isolated atrazine-degrading bacteria obtained from Europe and the United States contained homologs to the atz genes identified in Pseudomonas sp. ADP. The identity of the sequences were very high, being greater than 98% in all pairwise comparisons. This indicates that many atrazine-degrading bacteria worldwide metabolize atrazine via a pathway that proceeds through hydroxyatrazine, a metabolite which is non-phytotoxic and non-toxic to mammals. Enzymes were immobilized and used for degradation of atrazine in aqueous phases. The in-depth understanding of the genomics and biochemistry of the atrazine mineralization pathway enabled us to study factors affecting the prevalence of atrazine degradation in various agricultural soils under conservative and new agricultural practices. Moreover, Pseudomonas sp. ADP and/or its enzymes were added to atrazine-contaminated soils, aquifers and industrial wastewater to increase the rate and extent of atrazine biodegradation above that of untreated environments. Our studies enhance the ability to control the fate of regularly introduced pesticides in agriculture, or to reduce the environmental impact of unintentional releases.
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Siedow, J. (Molecular studies of functional aspects of plant mitochondrial proteins): (Annual performance report for 1988--1989). Office of Scientific and Technical Information (OSTI), enero de 1989. http://dx.doi.org/10.2172/5963065.

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Madhukar, A. Kinetic Aspects of Lattice Mismatch in Molecular Beam Epitaxial Growth on Planar and Patterned Substrates. Fort Belvoir, VA: Defense Technical Information Center, noviembre de 1990. http://dx.doi.org/10.21236/ada231636.

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Dawson, William O., Moshe Bar-Joseph, Charles L. Niblett, Ron Gafny, Richard F. Lee y Munir Mawassi. Citrus Tristeza Virus: Molecular Approaches to Cross Protection. United States Department of Agriculture, enero de 1994. http://dx.doi.org/10.32747/1994.7570551.bard.

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Citrus tristeza virus (CTV) has the largest genomes among RNA viruses of plants. The 19,296-nt CTV genome codes for eleven open reading frames (ORFs) and can produce at least 19 protein products ranging in size from 6 to 401 kDa. The complex biology of CTV results in an unusual composition of CTV-specific RNAs in infected plants which includes multiple defective RNAs and mixed infections. The complex structure of CTV populations poses special problems for diagnosis, strain differentiation, and studies of pathogenesis. A manipulatable genetic system with the full-length cDNA copy of the CTV genome has been created which allows direct studies of various aspects of the CTV biology and pathology. This genetic system is being used to identify determinants of the decline and stem-pitting disease syndromes, as well as determinants responsible for aphid transmission.
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Gafni, Yedidya y Vitaly Citovsky. Inactivation of SGS3 as Molecular Basis for RNA Silencing Suppression by TYLCV V2. United States Department of Agriculture, noviembre de 2013. http://dx.doi.org/10.32747/2013.7593402.bard.

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The Israeli isolate of Tomato yellow leaf curl geminivirus(TYLCV-Is) is a major tomato pathogen, causing extensive crop losses in Israel and in the south-eastern U.S. Yet, little is known about the molecular mechanisms of its interaction with tomato cells. One of the most interesting aspects of such interaction is how the invading virus counteracts the RNA silencing response of the plant. In the former BARD project, we have shown that TYLCV-Is V2 protein is an RNA silencing suppressor, and that this suppression is carried out via the interaction of V2 with the SGS3 component of the plant RNA silencing machinery. This reported project was meant to use our data as a foundation to elucidate the molecular mechanism by which V2 affects the SGS3 activity. While this research is likely to have an important impact on our understanding of basic biology of virus-plant interactions and suppression of plant immunity, it also will have practical implications, helping to conceive novel strategies for crop resistance to TYLCV-Is. Our preliminary data in regard to V2 activities and our present knowledge of the SGS3 function suggest likely mechanisms for the inhibitory effect of V2 on SGS3. We have shown that V2 possess structural and functional hallmarks of an F-box protein, suggesting that it may target SGS3 for proteasomal degradation. SGS3 contains an RNA-binding domain and likely functions to protect the cleavage produces of the primary transcript for subsequent conversion to double-stranded forms; thus, V2 may simply block the RNA binding activity of SGS3. V2 may also employ a combination of these mechanisms. These and other possibilities were tested in this reported project.
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Moore, Gloria A., Gozal Ben-Hayyim, Charles L. Guy y Doron Holland. Mapping Quantitative Trait Loci in the Woody Perennial Plant Genus Citrus. United States Department of Agriculture, mayo de 1995. http://dx.doi.org/10.32747/1995.7570565.bard.

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As is true for all crops, production of Citrus fruit is limited by traits whose characteristics are the products of many genes (i.e. cold hardiness). In order to modify these traits by marker aided selection or molecular genetic techniques, it is first necessary to map the relevant genes. Mapping of quantitative trait loci (QTLs) in perennial plants has been extremely difficult, requiring large numbers of mature plants. Production of suitable mapping populations has been inhibited by aspects of reproductive biology (e.g. incompatibility, apomixis) and delayed by juvenility. New approaches promise to overcome some of these obstacles. The overall objective of this project was to determine whether QTLs for environmental stress tolerance could be effectively mapped in the perennial crop Citrus, using an extensive linkage map consisting of various types of molecular markers. Specific objectives were to: 1) Produce a highly saturated genetic linkage map of Citrus by continuing to place molecular markers of several types on the map. 2) Exploiting recently developed technology and already characterized parental types, determine whether QTLs governing cold acclimation can be mapped using very young seedling populations. 3) Determine whether the same strategy can be transferred to a different situation by mapping QTLs influencing Na+ and C1- exclusion (likely components of salinity tolerance) in the already characterized cross and in new alternative crosses. 4) Construct a YAC library of the citrus genome for future mapping and cloning.
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