Tesis sobre el tema "Mlo genes"
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Geike, Juliane [Verfasser]. "Funktionelle Analyse von MLO Genen im Rosengenom / Juliane Geike". Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2018. http://d-nb.info/1172414211/34.
Texto completoCóser, Virgínia Maria. "Caracterização dos genes envolvidos nos rearranjos do gene MLL em leucemia aguda de novo de lactentes". reponame:Repositório Institucional da UFPR, 2013. http://hdl.handle.net/1884/32101.
Texto completoDorrance, Adrienne M. "The role of the partial tandem duplication of the MLL (MLL PTD) in leukemogenesis". Columbus, Ohio : Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1203712889.
Texto completoLima, Diego Silva. "Avaliação dos genes MLL, RB e TP53 em pacientes com síndrome mielodisplásica". reponame:Repositório Institucional da UFC, 2011. http://www.repositorio.ufc.br/handle/riufc/6887.
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Myelodysplastic syndromes (MDS) represent a heterogeneous group of clonal disorders affecting the hematopoietic pluripotent cell, characterized by low cell counts in peripheral blood, dysplasia in one or more cell lines, inefficient hematopoiesis and increased risk of progression to acute myeloid leukemia. Although the disease can affect patients of other age groups, they are more frequent in those with advanced age with an average 60 to 75 years at diagnosis. Chromosomal abnormalities are observed in approximately 50% of all cases of MDS and are related with clinical and morphological findings. The aim of this study was to determine, through the technique of FISH (fluorescence in situ hybridization), the frequency of changes involving the MLL, RB, and TP53 genes in patients with MDS and associate these changes with cytogenetic findings. The cases included in the study were selected in the ambulatory of SMD from University Hospital Walter Cantídio. Thirty three patients were selected, 17 had aged over 60 years. 52% of patients were classified, according to WHO criteria, as refractory cytopenia with dysplasia in multiple lineages (RCDM) and 61% stratified, according to IPSS, as intermediate risk 1 (INT-1). 78% of patients had abnormalities detected by cytogenetics, among them 31% had complex karyotypes (more than 3 changes per metaphase). 18% of patients had changes at least in one of the three genes valued in this study by FISH. Three patients showed alterations of TP53 gene, being detected in two patients (records 31 and 6) the deletion of a single allele or both alleles of the gene, respectively, and in the third (record 2), we detected amplification of TP53 gene, all this changes were not detected by classical cytogenetics, because it is a less sensitive technique. 6% of patients (records 7 and 22) had rearrangement of MLL gene. In the first case, FISH discarded the gene deletion alleged by cytogenetic, proving that it was present in the genome of the patient, but in a rearranged form, and in the second case cytogenetics failed to demonstrate rearrangement of the gene. For the RB gene, FISH identified only one patient (3%) with deletion of one allele of the gene, and this change was also not detected by classical cytogenetics. During evaluating the TP53 gene, FISH allowed identification of two patients (records 5 and 10) presenting at least six extra copies of chromosome 17, probably representing a small hyperdiploid clone partially detected in the first patient and not detected in the second . In the six patients who showed abnormalities of the genes analyzed, FISH has provided information that added, changed or confirmed the result previously given by classical cytogenetics, which are a major application of this technique due to its high sensitivity compared to the traditional method.
As síndromes mielodisplásicas (SMD) representam um grupo heterogêneo de doenças clonais que acometem a célula precursora hematopoética pluripotente, caracterizando-se por baixa contagem de células no sangue periférico, displasia em uma ou mais linhagens celulares, hematopoese ineficiente, além do risco aumentado de progressão para leucemia mielóide aguda. Embora a doença possa acometer pacientes de outras faixas etárias, é mais frequente naqueles com idade avançada, com média ao diagnóstico de 60 a 75 anos. As anormalidades cromossômicas são observadas em aproximadamente 50% de todos os casos de SMD, estando, em alguns casos, relacionadas com achados clínicos e morfológicos. O objetivo deste trabalho foi determinar, através da técnica de FISH (hibridização in situ por fluorescência), a frequência de alterações envolvendo os genes MLL, RB e TP53 em pacientes com SMD e associar estas alterações com os achados citogenéticos. Os casos inseridos no estudo foram oriundos do ambulatório de SMD do Hospital Universitário Walter Cantídio. Dos 33 pacientes selecionados, 17 pertenciam ao grupo com idade acima de 60 anos. 52% dos pacientes foram classificados, segundo a OMS, como citopenia refratária com displasia em múltiplas linhagens (CRDM) e 61% estratificados, segundo o IPSS, como de risco intermediário 1 (INT-1). Um total de 78% dos pacientes apresentaram alterações citogenéticas, dentre eles 31% possuíam cariótipos complexos (mais de 3 alterações por metáfase). A técnica de FISH permitiu identificar em 18% dos pacientes alterações envolvendo um dos três genes avaliados. Três pacientes apresentaram alteração do gene TP53, sendo detectada em dois deles (registros 31 e 6) a deleção de um único alelo ou de ambos os alelos do gene, respectivamente, e no terceiro (registro 2), detectou-se a amplificação do gene TP53, sendo estas alterações não visualizadas através da citogenética clássica, por se tratar de um técnica menos sensível. Detectou-se em 6% dos pacientes (registros 7 e 22) rearranjo do gene MLL, no primeiro a FISH descartou a suposta deleção do gene alegada pela citogenética, provando que o mesmo estava presente no genoma do paciente, porém de forma rearranjada e no segundo a citogenética não conseguiu demonstrar o rearranjo do gene. Quanto ao gene RB, a FISH permitiu identificar apenas um paciente (3%) com deleção de um dos alelos do gene, sendo esta alteração também não detectada pela citogenética clássica. A FISH possibilitou identificar, durante a avaliação do gene TP53, dois pacientes (registros 5 e 10) apresentando pelo menos 6 cópias extras do cromossomo 17, devendo essa alteração se tratar de um pequeno clone hiperdiplóide detectado parcialmente no primeiro paciente e não detectado no segundo. Nos seis pacientes que apresentaram alteração dos genes avaliados, a FISH proveu informações que adicionaram, confirmaram ou alteraram o resultado previamente emitido pela citogenética clássica, sendo estas uma das principais aplicações desta técnica devido sua alta sensibilidade quando comparada ao método clássico.
Makepeace, Joanne Claire. "The effect of the mlo mildew resistance gene on spotting diseases of barley". Thesis, University of East Anglia, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.437638.
Texto completoBussiere, Marianne. "Characterising the MLL complex : epigenetic regulation of Hoxa genes". Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/707/.
Texto completoWong, Piu. "Meis1 and micrornas as collaborating genes in MLL leukemia /". May be available electronically:, 2008. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.
Texto completoCleavinger, Peter Jay. "Role of the long terminal repeat in transcriptional regulation of rous sarcoma virus gene expression". free to MU campus, to others for purchase, 1996. http://wwwlib.umi.com/cr/mo/fullcit?p9841207.
Texto completoNigavekar, Shraddha S. "Regulation of GLC7 encoded PP1 and analysis of synthetic lethal interactions with ade3 and leu2 in saccharomyces cerevisiae". free to MU campus, to others for purchase, 2001. http://wwwlib.umi.com/cr/mo/fullcit?p3013007.
Texto completoAggelis, Alexandros. "Gene expression in ripening melon (Cucumis melo L.)". Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.319646.
Texto completoLima, Diego Silva. "AvaliaÃÃo dos genes MLL, RB e TP53 em pacientes com sÃndrome mielodisplÃsica". Universidade Federal do CearÃ, 2011. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6770.
Texto completoMyelodysplastic syndromes (MDS) represent a heterogeneous group of clonal disorders affecting the hematopoietic pluripotent cell, characterized by low cell counts in peripheral blood, dysplasia in one or more cell lines, inefficient hematopoiesis and increased risk of progression to acute myeloid leukemia. Although the disease can affect patients of other age groups, they are more frequent in those with advanced age with an average 60 to 75 years at diagnosis. Chromosomal abnormalities are observed in approximately 50% of all cases of MDS and are related with clinical and morphological findings. The aim of this study was to determine, through the technique of FISH (fluorescence in situ hybridization), the frequency of changes involving the MLL, RB, and TP53 genes in patients with MDS and associate these changes with cytogenetic findings. The cases included in the study were selected in the ambulatory of SMD from University Hospital Walter CantÃdio. Thirty three patients were selected, 17 had aged over 60 years. 52% of patients were classified, according to WHO criteria, as refractory cytopenia with dysplasia in multiple lineages (RCDM) and 61% stratified, according to IPSS, as intermediate risk 1 (INT-1). 78% of patients had abnormalities detected by cytogenetics, among them 31% had complex karyotypes (more than 3 changes per metaphase). 18% of patients had changes at least in one of the three genes valued in this study by FISH. Three patients showed alterations of TP53 gene, being detected in two patients (records 31 and 6) the deletion of a single allele or both alleles of the gene, respectively, and in the third (record 2), we detected amplification of TP53 gene, all this changes were not detected by classical cytogenetics, because it is a less sensitive technique. 6% of patients (records 7 and 22) had rearrangement of MLL gene. In the first case, FISH discarded the gene deletion alleged by cytogenetic, proving that it was present in the genome of the patient, but in a rearranged form, and in the second case cytogenetics failed to demonstrate rearrangement of the gene. For the RB gene, FISH identified only one patient (3%) with deletion of one allele of the gene, and this change was also not detected by classical cytogenetics. During evaluating the TP53 gene, FISH allowed identification of two patients (records 5 and 10) presenting at least six extra copies of chromosome 17, probably representing a small hyperdiploid clone partially detected in the first patient and not detected in the second . In the six patients who showed abnormalities of the genes analyzed, FISH has provided information that added, changed or confirmed the result previously given by classical cytogenetics, which are a major application of this technique due to its high sensitivity compared to the traditional method.
Fleischmann, Katrin Kristina. "Identification of MLL-A9 related target genes and microRNAs involved in leukemogenesis". Diss., Ludwig-Maximilians-Universität München, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-161310.
Texto completoChen, Di. "Regulatory pathways controlling larval development in caenorhabditis elegans". Free to MU Campus, others may purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3144405.
Texto completoSilva-Barreto, Fatima Aparecida da. "Identificação de marcador molecular ligado ao gene Pm-1 que confere resistência a raça 1 de oídio (Podosphaera xanthii) em melão (Cucumis melo L.)". Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/11/11135/tde-08082007-171137/.
Texto completoMelon crop is of great economical importance in Brazil being the Northeast region the main producer and exporter of the fruit. The low levels of resistance to pathogens is one of the restricting factors for the Brazilian competitiveness worldwide. One of the most important diseases is powdery mildew caused by Podosphaera xanthii. Generally, the control of P. xanthii is achieved with the use of fungicides. However it is necessary to use less expensive control methods with a minimum environmental impact such as resistant cultivars. The objective of this work was to identify molecular markers linked to the Pm-1 gene which confers resistance to race 1 of P. xanthii with the purpose of assisting boding program. Two near isogenic lines (LQIs) of melon Agro AF426pm1 (P1) and AF426Pm1 (P2), both belonging to the inodorus variety, which are respectivally contrasting for abscence and presence of Pm-1 gene were analyzed. For the cosegregation analyses between gene and candidate markers, it was used the BC1F1 population was used screened for resistance to powdery mildew and obtained from a cross between the LQIs. The LM-PCR and AFLP techniques were efficient in the polymorphisms detection, however only those ones which were found using AFLP technique could be used as markers in the co-segregation test. It was found two markers with this technique but just the HF155 is located 4.9 cM of the Pm-1 gene. Due the proximity of the HF155 marker to the Pm-1 gene this one can be used in markerassisted selection aiming to develop melon cultivars resistant to P. xanthii.
Ng, Ming-him. "Ras signalling pathway and MLL-rearranged leukaemias". View the Table of Contents & Abstract, 2006. http://sunzi.lib.hku.hk/hkuto/record/B3643419X.
Texto completoNg, Ming-him y 吳明謙. "Ras signalling pathway and MLL-rearranged leukaemias". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37238656.
Texto completoWassano, Débora Targino. "Identificação de marcadores ligados a genes de resistência à multiplicação de Zucchini yellow mosaic virus (ZYMV) em meloeiro". Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-20032014-170310/.
Texto completoViruses cause major damage to cucurbits due to the severity of the symptoms and because there are no curative methods of control. Among the viruses that infect muskmelon, ZYMV is one of the most severe and frequent. PI414723 is the only source of resistance to this pathogen, being resistant to the pathotype 0. Resistance is conferred by a single dominant gene, Zym-1, but there are reports on the existence of at least two others (Zym-2 and Zym-3). To date, only Zym-1 was located on a linkage map but there is evidence that Zym-2 is linked to the microsatellite marker CMCT134b. The objective of this study was to identify markers linked to Zym-2 from linkage analysis between microsatellite loci and resistance to the virus in F2 populations derived from the cross PI414723 x \' Védrantais \'. The inoculum was isolated from a commercial field and characterized as to its aggressiveness, pathotype and transmissibility by aphids. Plants were inoculated mechanically and resistance was evaluated by measuring the viral titer by PTA- ELISA. The distribution of viral titers was not normal and showed tendency for low values, indicating the presence of at least one dominant gene of large effect. The linkage of Zym-1 to CMAG36 was confirmed by linear regression. Plants homozygous for the \'Vedrantais\' marker allele on this locus were genotyped with microsatellite markers linked to CMCT134b and the linkage of these with Zym-2 were tested by simple linear regression. The analyses indicated a significant association between viral titers and genotypes at the CMBR55 and CMGA172 marker loci. Regions of linkage groups II and X were constructed with five markers each in order to locate Zym-1 and Zym-2 by the Multiple Intervals Mapping approach (MIM). Linkage between Zym-1 and CMAG36 was confirmed at an estimated distance of 3.4 cM (LOD = 33.32 ), as expected, while Zym-2 was found linked to CMBR55 at an estimated distance of 3.9 cM (LOD = 17.45). Epistasis between Zym-1 and Zym-2 was found (LOD=12.73), indicating that the phenotype of plants homozygous for \'Vedrantais\' marker allele in CMAG36 depended on their genotype in CMBR55. A second F2 population derived from the same cross was phenotyped for resistance to ZYMV and genotyped with the same markers in order to validate the results. The linkage of Zym-1 to CMAG36 was validated, but the same did not occur with Zym-2. This results probably from the fact that Zym-2 presented minor phenotypic effects which are harder to detect due to environmental variations. In addition, the use of few markers probably contributed to this result as well, since the number of markers influences the power to detect quantitative loci. Besides providing further evidences of the existence of a second resistance gene in PI414723, this study also detected epistatic effects between them. Notwithstanding, it is concluded that resistant cultivars can be developed from PI414723 solely based on the selection assisted with CMAG36.
Yap, Wee Ching Melvyn. "Analysis of retroviral production in murine leukaemia virus". Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325497.
Texto completoMorris, Erin Rebecca. "FHA domain genes of Arabidopsis /". free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3144443.
Texto completoGuimarães, Larissa Oliveira. "Caracterização de subpopulações de Leucemia Mielóide Aguda portadora do rearranjo MLL quanto à resposta diferencial ao tratamento em longo prazo com Citarabina". Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-07012016-115206/.
Texto completoThe heterogeneity of Acute Myeloid Leukemia (AML) became a challenge for the success of the conventional chemotherapy agent Cytarabine (Ara-C), especially in leukemias with poor prognosis, as those harboring MLL rearrangement. Since AML-MLL cells are considered sensitive to Ara-C when compared with leukemias that do not carry the rearrangement, but relapse is frequent, the present dissertation proposed to study the relationship between biological characteristics related to the basis of chemoresistance to Ara-C in AML-MLL. We proposed an approach based on the selection of subpopulations of cell lines bearing MLL rearrangement submitted to the long-term treatment with Ara-C, comparing them with the cell lines that were not previously exposed to the drug. The cells were characterized according to: 1) the proliferative potential in the presence and absence of Ara-C; 2) the distribution of the cells in the cell cycle; 3) distribution of hematopoietic stem cell classic surface markers, CD34 and CD38; and, 4) global expression profile of transcribed RNAs. The long-term treatment selected cells that are more resistant to Ara-C than the cells that were not previously treated (parental cells). Besides, according to cell cycle, the cells selected by Ara-C treatment present decreased apoptosis (sub-G1 phase), accumulation in the synthesis phase (S-phase) and increase in the proliferative capability after re-exposition to the drug (G2-M phase). Regarding the hematopoietic stem cell markers, we observed that after Ara-C long-term treatment, one of the cell lines exhibited a bimodal distribution of the CD38 marker. When sorted by flow cytometry, we observed that both subpopulations with distinct levels of CD38 expression, called MV-4-11 CD38High and MV-4-11 CD38Low also showed distinct response to Ara-C. When evaluated regarding to their global gene expression profiles, we verified that MV-4-11 CD38High were more closely related to the parental cells, and MV-4-11 CD38Low made up an isolated group, distinct of the other cell populations. Gene ontology (GO) analysis revealed that among the most representative categories of biological processes, activities associated with proliferative capability, development and response to stimuli were included. The hierarchical clustering analysis showed that: 1) the cluster HOXA of genes of development was more expressed in the MV-4-11 CD38Low than in the MV-4-11 CD38High cells, that presented increased expression of HOXB cluster; 2) the most differentially expressed HOX gene was HOXA13, which according to the literature is associated with poor prognosis in other types of cancer; 3) among the genes associated with response to stimuli, the only one related to Ara-C-metabolizing pathway that was differentially expressed between the cell lines was NME1; 4) those genes that take part in the mismatch repair, base excision repair and nucleotide excision repair pathways were more expressed in the MV-4-11 CD38High than in the MV-4-11 CD38Low cells. Additionally, several cyclin-dependent kinases (CDKs) were also differentially expressed between MV-4-11 CD38High and MV-4-11 CD38Low. Finally, we suggest that the in vitro model proposed in this study to mimic the situation of chemoresistance to Ara-C in subpopulations of AML-MLL, showed that the mechanisms of Ara-C response in this disease, go beyond changes in drug detoxification and metabolization, and seem more associated to proliferative and development advantages of the leukemic cells. These pathways should be explored as potential targets to Ara-C combination therapies.
Leis, Thomas. "Charakterisierung genomischer Bruchpunkte innerhalb des MLL-Gens bei Leukämien im Kindesalter". [S.l.] : [s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961722878.
Texto completoTrentin, Luca. "Microarray Analysis: a Leading Tool in the Classification and Biological Characterization of Pediatric Onco-Hematological Diseases". Doctoral thesis, Università degli studi di Padova, 2010. http://hdl.handle.net/11577/3427012.
Texto completoL’analisi del profilo d’espressione genica mediante microarray rappresenta uno strumento utile per la classificazione delle leucemie in ambito diagnostico, l’identificazione di nuove sottoclassi di malattia e l’associazione di profili d’espressione genica con la prognosi. I molteplici lavori pubblicati nell’ambito delle malattie onco-ematologiche sia nell’adulto che nel bambino hanno evidenziato la robustezza della tecnologia microarray ed auspicano, quindi, l’ utilizzo dei microarrays in affiancamento alle metodiche “gold standard” per la diagnosi di leucemia. Considerando che la qualità dell’RNA di partenza è un fattore determinante per la buona riuscita di un esperimento di studio dell’espressione genica, abbiamo valutato se diverse metodiche di isolamento dell’RNA avessero una qualche influenza sulla variazione del profilo d’espressione genica. I risultati ottenuti nel nostro studio, analizzando diversi sottotipi di leucemie pediatriche, hanno evidenziato che le metodiche impiegate per l’estrazione dell’RNA non vanno ad influire sul profilo d’espressione genico e che quest’ultimo rimane, comunque, ben identificabile a prescindere dalla metodologia usata per l’isolamento dell’RNA. Applicando, poi, l’analisi microarrays alle leucemie a cellule precursori B e con traslocazione MLL/AF4, abbiamo individuato, all’interno di questo sottotipo di leucemia ritenuto fino ad ora omogeneo, due sottogruppi di pazienti caratterizzati da un differente profilo d’espressione genica in cui spiccava la diversa espressione dei geni HOXA. Questo risultato è alquanto sorprendente poiché la maggiore espressione dei geni HOXA è una caratteristica distintiva delle leucemie con riarrangiamento del gene MLL. Non abbiamo identificato nessuna altra variazione d’espressione di geni (per es. MENIN, HOXC8 e MEIS1) comunemente associati con le leucemie con riarrangiamento del gene MLL. Anche l’analisi del profilo dell’espressione dei microRNA ha dimostrato che questi pazienti possono essere suddivisi in due sottogruppi ben distinti ed, inoltre, ha evidenziato che i pazienti con bassa espressione dei geni HOXA non esprimono il microRNA mir-196b, che è localizzato nel medesimo cluster dei geni HOXA e che è coinvolto nei processi di leucemogenesi. Lo studio del profilo d’espressione genica di pazienti affetti da leucemia mielomonocitica giovanile (JMML) ci ha, poi, consentito di dividere i campioni analizzati in due sottogruppi. Questa suddivisione è associata, in modo altamente significativo, con la prognosi di malattia. Il medesimo risultato prognostico non è conseguibile prendendo in considerazione i fattori prognostici clinici standard (emoglobina fetale, età alla diagnosi e conta piastrinica). Infine, abbiamo studiato il ruolo del gene SOCS-2 nelle leucemie con riarrangiamento MLL/AF4. Questo gene, up-regolato nelle cellule staminali, è uno dei geni maggiormente espressi nei pazienti con MLL/AF4 rispetto ai controlli normali. Il silenziamento di SOCS-2 nelle cellule RS4;11 determina un aumento dell’apoptosi rispetto alle cellule silenziate con un siRNA di controllo ed una simultanea maggiore espressione di TP53 e BAX. L’over-espressione di SOCS-2 nelle cellule RS4;11 sembra essere, quindi, un meccanismo in grado di aumentare la sensibilità di queste cellule all’apoptosi. L’analisi dell’espressione di SOCS-2 in più pazienti affetti da leucemia linfoblastica acuta (LLA) ha evidenziato che SOCS-2 è over-espresso in tutte le LAL tranne le LAL a cellule T. Questo dato suggerisce che l’azione anti-apoptotica di SOCS-2 potrebbe essere comune in più sottotipi di leucemia.
Green, Jonathan A. "Trophoblast-expressed genes within the ungulates /". free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9842531.
Texto completoFazza, Ana Carolina. "Mapeamento de genes de resistência a três raças de Podosphaera xanthii em meloeiro (Cucumis melo L.)". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-23052011-155253/.
Texto completoMelon (Cucumis melo L.) is a crop of great economic importance for the export trade in Brazil and is cultivated mainly in the Northeast. Crop yield can be affected by a disease of the aerial parts, called powdery mildew that in Brazil is caused by the fungus Podosphaera xanthii. This pathogen has several races characterized based on the reaction of a set of differential melon cultivars. Among these genotypes, the plant introgression PI 414723 is resistant to most races and the breeding line Védrantais is susceptible. This study aimed to: (i) study the inheritance of resistance to races 1, 3 and 5 of P. xanthii in the F2 generation from the cross PI 414723 x Védrantais, and (ii) map resistance genes to these races in this same population based on amplified fragment length polymorphism (AFLP), simple sequence repeat (SSR) and resistance gene analog (RGA) markers. The inheritance of resistance was analyzed on 87 F2 individuals grown under greenhouse conditions. The three races were inoculated simultaneously on four leaves of each plant. Plants were classified as resistant or susceptible based on visual assessments of fungal growth on the leaves. Plants were considered susceptible when there was abundant production of conidia and resistant when the production was scarce or non-existent. The frequencies of resistant and susceptible individuals indicated that resistance to all three races is controlled by a dominant major gene. A genetic map was constructed comprising 1469 cM, consisting of 207 markers (139 AFLP, 47 SSR, 18 RGA, and three phenotypic) with an average distance of 7.4 cM between markers distributed in 12 linkage groups. Co-segregation analysis with markers indicated that the resistance genes are located on linkage group II. Moreover, the analysis indicated complete linkage between resistance to races 1 and 5, and this gene was denominated Pm-x1.5. The gene for resistance to race 3 (Pm-x3) was located at 5.1 cM from Pmx1.5. An AFLP marker (H35M75_156) was located between the two genes at 1.3 cM from Pmx1.5 and 3.8 cM from Pm-x3. These is the first report on the location of resistance genes to races 3 and 5 in PI 414723, and also the first report of RGA markers mapping using the TRAP technique in melon.
Högkvist, Linda. "”För man får va som man vill” : Barns uppfattning om könsroller utifrån högläsning och diskussion om Mio min Mio av Astrid Lindgren och Snäll av Gro Dahle och Svein Nyhus". Thesis, Karlstads universitet, Institutionen för språk, litteratur och interkultur, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-31301.
Texto completoAs fellow people and in the teaching profession, I believe that we need to have a good understanding about gender, because it affects our educational basic view and how we behave as humans. To be able to constructively challenge the current gender norms, I believe that we need to understand our pupils understanding in the subject of gender. We also need to understand their thoughts about the differences between boys and girls in a gender perspective. The study is about the pupils thoughts of gender and gender norms after reading aloud and having discussions about Astrid Lindgrens Mio, my son (2011/ 1954) and Gro Dahle & Svein Nyhus Snäll (Kind authors translation) (2008). Mio in Mio, my son (Lindgren 2011/ 1954) represent the kind, but brave boy as a main character and Lussi in Snäll (Dahle & Nyhus 2008) represents the kind, but norm breaking girl as the main character. The main issues are how pupils in the 6th grade interpret the chosen literature from a gender perspective, and how it affects the students in their thoughts about gender and gender norms. What is interesting is that in every time with its own culture and history, there is room for change (Hedlin 2010 s.20). Does this change happen when the pupil interacts with the books? The pupils have answered a questionnaire about the gender perspective in each book, and I chose to analyze the questionnaires of six pupils, three boys and three girls. The results show that the pupils have seen a clear gender perspective in the literature and on this basis, they developed their understanding and ultimately, they have been able to formulate their thoughts about gender and gender roles on a deeper level.
SALLES, Terezinha de Jesus Marques. "Estudos citogenéticos nas leucemias do lactente". Universidade Federal de Pernambuco, 2010. https://repositorio.ufpe.br/handle/123456789/6024.
Texto completoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
A análise citogenética convencional e molecular nas leucemias do lactente (LL) é de fundamental importância para estabelecer alterações cromossômicas e que definem grupos e subgrupos de risco. Este estudo caracterizou alterações cromossômicas em 83 lactentes, oriundos do Centro de Oncohematologia do Hospital Universitário Oswaldo Cruz e Centro de Transplante de Medula Óssea do Instituto Nacional do Cancer (CEMO/INCA) no período de julho/2000 a agosto/2010, sendo 73 casos de leucemias agudas (LA) e 10 de síndrome mielodisplásica (SMD). As Leucemias agudas foram classificadas como leucemia linfoblástica aguda (LLA) (47 casos), leucemia mielóide aguda (LMA) (23 casos) e leucemias ambíguas (3 casos). A idade dos pacientes com leucemia linfoblástica aguda variou de 5 dias a 22 meses, com proporção entre os sexos de 1:1. A idade dos casos de leucemia mielóide aguda variou de 2 a 21 meses, sendo 15 masculinos e 7 femininos. Os subtipos morfológicos foram leucemia mielóide com diferenciação (M2), leucemia mielomonocítica (M4), leucemia mielomonocítica eosinofílica (M4eo), leucemia monoblástica (M5) e leucemia megacarioblástica (M7). O bandeamento G (GBG) revelou anormalidades da região 11q23 em 38% dos casos de leucemia linfoblástica e em 50% dos casos de leucemia mieloblástica. O rearranjo do gene MLL por hibiridização in situ foi observado em 65% e 39% das leucemias linfoblásticas e leucemia mielóide, respectivamente. Nos casos com síndrome mielodisplásicas, a idade variou entre 20 dias a 20 meses, sendo seis do sexo masculino e quatro do feminino. Sete casos foram leucemia mieloblástica em portadores de S.Down, destes seis eram leucemias megacarioblásticas (LMA-K/LMA-M7) e uma leucemia mielóide sem diferenciação (LMA-MO). Todas as leucemias megacarioblásticas tiveram cariótipos complexos com translocações envolvendo o cromossomo 1. Os métodos de Hibridização in situ (FISH), multicolor FISH (M-FISH) e bandeamento cromossômico multicolorido (MCB), foram essenciais nos casos duvidosos pelo GBG, nos cariótipos complexos e nos casos de cromossomos marcadores
Tsang, Hon-man. "Studies of Mll-Een fusion gene in a conditional mouse model of human leukemia". Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39558034.
Texto completoLui, Wing-chi y 呂穎芝. "Identification of leukemia-associated genes by MLL-EEN fusion protein through dysregulation of histone modification and DNA methylation". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hdl.handle.net/10722/196088.
Texto completopublished_or_final_version
Pathology
Doctoral
Doctor of Philosophy
Fleischmann, Katrin Kristina [Verfasser] y Elisabeth [Akademischer Betreuer] Weiss. "Identification of MLL-AF9 related target genes and microRNAs involved in leukemogenesis / Katrin Kristina Fleischmann. Betreuer: Elisabeth Weiss". München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2012. http://d-nb.info/1042374007/34.
Texto completoSchuh, Artur Francisco Schumacher. "Farmacogenética da levodopa na doença de Parkinson : estudo de polimorfismos nos genes da COMT, MAO-B e DAT". reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2010. http://hdl.handle.net/10183/39639.
Texto completo曾漢文 y Hon-man Tsang. "Studies of Mll-Een fusion gene in a conditional mouse model of human leukemia". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39558034.
Texto completoGracia-Maldonado, Gabriel. "Exploiting the MLL-rearranged leukemia gene signature to identify molecular targets for novel therapies". University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1573570752309466.
Texto completoLargeot, Anne. "Contrôle de l'expression du gène HOXA9 dans les cellules souches/progénitrices hématopoïétiques : rôle des enzymes épigénétiques MOZ et MLL, et du facteur de polyadénylation Symplekin". Thesis, Dijon, 2013. http://www.theses.fr/2013DIJOS080/document.
Texto completoMy thesis project has consisted of the study of MOZ, and MLL. They are epigenetic regulators. MOZ and MLL activate transcription of HOX genes, which are transcription factors essential during haematopoiesis. MOZ and MLL have some target genes in common. In our study, we characterised a cooperation between MOZ and MLL in human haematopoietic stem/progenitor cells CD34+. They are both recruited onto HOX promoters. MOZ is essential for MLL recruitment, and this is reciprocal. In conclusion, we provided an example of a mechanism involving a direct cross-talk between two histone modifying enzymes.In order to dissect the mechanism of action of this complex, we decided to identify novel proteins interacting with both MOZ and MLL. A member of the RNA polyadenylation machinery has been isolated: Symplekin. We confirmed the interaction between MOZ, MLL and Symplekin in the human haematopoietic immature cell line KG1. We showed that Symplekin is co-recruited to HOXA9 promoter along with MOZ and MLL. We demonstrated the dual role of this member of the polyadenylation machinery. Indeed, besides the fact that Symplekin is important for Hoxa9 polyadenylation, thus its stability, it prevents MOZ and MLL recruitment onto HOXA9 promoter, leading to a decrease of HOXA9 transcription.Our work improved the understanding of the mechanism of action of MOZ and MLL in HOX control
Mason, Lyndel Ann. "Expression variation in lysosomal storage disorder genes". Thesis, Queensland University of Technology, 2006. https://eprints.qut.edu.au/16240/1/Lyndel_Mason_Thesis.pdf.
Texto completoLammers, Mark C. "Genus n Banach spaces /". free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841162.
Texto completoMason, Lyndel Ann. "Expression variation in lysosomal storage disorder genes". Queensland University of Technology, 2006. http://eprints.qut.edu.au/16240/.
Texto completoTu, Liwen. "Cloning and sequence analysis of multiple genes from Bifidobacterium infantis /". free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3137758.
Texto completo江卓庭 y Cheuk-ting Kong. "Understanding the function of the Mll-een leukaemic fusion gene by embryonic stem cell approaches". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31244312.
Texto completoCova, Valentina <1978>. "Isolamento di marcatori microsatelliti strettamente associati al gene di resistenza a ticchiolatura Vm in melo". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2008. http://amsdottorato.unibo.it/723/1/Tesi_Cova_Valentina.pdf.
Texto completoCova, Valentina <1978>. "Isolamento di marcatori microsatelliti strettamente associati al gene di resistenza a ticchiolatura Vm in melo". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2008. http://amsdottorato.unibo.it/723/.
Texto completoGassó, Astorga Patricia. "Estudio de asociación de polimorfismos en los genes "COMT, MAO-A, MAO-B" y "DAT" con el riesgo de esquizofrenia y de aparición de efectos extrapiramidales en pacientes tratados con antipsicóticos". Doctoral thesis, Universitat de Barcelona, 2008. http://hdl.handle.net/10803/1641.
Texto completoFactores genéticos determinan, en parte, tanto el riesgo de sufrir esquizofrenia como el de desarrollar EPS en pacientes tratados con APs. Una de las teorías fisiopatológicas de la esquizofrenia defiende la existencia de un exceso de actividad dopaminérgica en los cerebros de estos pacientes, dado que el principal mecanismo de acción de los APs es el bloqueo de los receptores dopaminérgicos. No obstante, este mismo bloqueo a nivel del estriado sería lo que desencadenaría la aparición de EPS. La homeostasis de la dopamina en la sinapsis se lleva a cabo mediante su recaptación por parte del transportador de dopamina (DAT) y mediante su metabolismo por parte de las enzimas Catecol-O-Metiltrasferasa (COMT) y Monoamino Oxidasa (MAO). Polimorfismos genéticos que den lugar a una menor actividad de COMT, MAO y DAT, y que por lo tanto determinarían una mayor disponibilidad de dopamina, darán lugar a un mayor riesgo de esquizofrenia y a un menor riesgo de EPS.
Los objetivos principales de esta tesis han sido realizar un estudio de asociación entre polimorfismos de COMT (G158A y -278 A/G), MAO-A (30bp-VNTR y 941T/G), MAO-B (A/G intrón 13) y DAT (40bp-VNTR y -67A/T), y de sus respectivos haplotipos, tanto con el riesgo de esquizofrenia como con el riesgo de aparición de síntomas extrapiramidales inducidos por antipsicóticos. Los objetivos secundarios fueron realizar un estudio descriptivo de las frecuencias genotípicas de estos polimorfismos en nuestra población general de referencia, y diseñar y poner a punto un método de genotipado multiplex para el análisis de los polimorfismos VNTR de MAO-A y DAT.
Para ello se reclutaron 500 pacientes del Servicio de Psiquiatría del Hospital Clínico de Barcelona entre los años 2002 y 2004. 243 fueron diagnosticados con esquizofrenia y trastornos relacionados, que junto con 291 controles de base hospitalaria, permitieron realizar el estudio de riesgo de esquizofrenia. 270 pacientes esquizofrénicos y con trastorno bipolar tratados con terapia antipsicótica se incluyeron en el estudio de riesgo de EPS. 81 casos desarrollaron extrapiramidalismo (Simpson Angus > 3) y 189 controles no desarrollaron esta sintomatología (Simpson Angus ≤ 3). A partir de una muestra de sangre entera de todos los participantes realizamos el aislamiento de ADN genómico. Los polimorfismos seleccionados se genotiparon mediante diferentes métodos validados incluyendo PCR-RFLP, ASO-PCR y PCR multiplexelectroforesis capilar.
En el estudio de riesgo de esquizofrenia, observamos que los individuos heterocigotos AG para el polimorfismo COMT -278A/G reducían el riesgo de sufrir la enfermedad en un 60% (OR: 0.4, p=0.009). Además detectamos al alelo G del polimorfismo MAO-B A/G intrón 13 como un factor de riesgo de esquizofrenia (OR:2.3 p=0.006). Cuando realizamos el análisis estratificando por sexo, el alelo G fue un factor de riesgo únicamente en mujeres (Alelo G, OR:2.6; homocigotas GG, OR:6.7; p=0.01). En cuanto al estudio de riesgo de EPS, el haplotipo A-A mostró ser un factor de protección pero únicamente en los pacientes bipolares (p=0.006).
Nuestros resultados sugieren que los polimorfismos COMT -278 A/G y and MAO-B A/G intrón 13 pueden contribuir al riesgo de desarrollar esquizofrenia. Además, este es el primer trabajo donde se encuentra una asociación entre polimorfismos en la COMT y la susceptibilidad a los EPS. Este resultado es especialmente interesante si tenemos en cuenta el incremento del uso de fármacos antipsicóticos en pacientes bipolares.
Schizophrenia is a multifactorial disease with an important genetic factor. Schizophrenic patients are treated with antipsychotic drugs (APs), which may cause extra pyramidal symptoms (EPS). Genetic factors may also predispose patients to develop this adverse effect. Both schizophrenia risk and EPS risk depend, partly, on brain dopamine levels. The homeostasis of this neurotransmitter is carried out by the action of the dopamine transporter (DAT) and the action of the Catechol-O-Methyltrasferase (COMT) and Monoamine Oxidase (MAO) enzymes. According to this, the aim of this research was to examine the relationship between the COMT (G158A and -278 A/G), MAO-A (30bp-VNTR and 941T/G), MAO-B (A/G intrón 13) and DAT (40bp-VNTR and -67A/T) polymorphisms, and their corresponding haplotypes, and the risk of schizophrenia as well as the risk of EPS.
243 patients with schizophrenia and related disorders and 291 controls were included in the study of schizophrenia risk. 270 patients receiving AP therapy (81 cases and 189 controls) were included in the study of EPS risk. Selected polymorphisms were genotyped following various validated methods, including PCR-RFLP, ASO-PCR and multiplex PCR-Capillary electrophoresis.
In the study of schizophrenia, the heterozygotes for the COMT -278A/G polymorphism had a reduced risk of suffering the disease (OR: 0.4, p=0.009). We also identified allele G of the MAO-B A/G intron 13 polymorphism as a risk factor for schizophrenia (OR:2.3 p=0.006). When we carried out a sex-specific analysis, the allele G was a risk factor only in women (Allele G, OR:2.6; homozygotes GG, OR:6.7; p=0.01). In the study of EPS risk, the A-A haplotype of COMT showed to be a protective factor, but only in the bipolar patients (p=0.006). These results suggest that the COMT -278 A/G and MAO-B A/G intron 13 polymorphisms may contribute to the risk of developing schizophrenia. Moreover, this is the first study of an association between COMT polymorphisms and EPS susceptibility. This result is especially interesting if we take into account the increased use of AP drugs in bipolar patients.
Lee, Sungkeun. "Molecular genetic analysis of nucleotide excision repair genes in Dictyostelium discoideum /". free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841209.
Texto completoWong, Christine. "Construction & Evaluation of a Reporter Gene Displaying Aldehydes on the Cell Surface". Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/41214.
Texto completoLi, Mao [Verfasser] y Carsten [Akademischer Betreuer] Schmuck. "Development of novel peptide based gene delivery vector and supramolecular assembly / Mao Li ; Betreuer: Carsten Schmuck". Duisburg, 2016. http://d-nb.info/1120923557/34.
Texto completoYu, Sung-Lim. "Analysis of the response of nucleotide excision repair genes in Dictyostelium discoideum /". free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841196.
Texto completoKuznicki, Kathleen. "The function of the germline rna helicase (GLH) genes in caenorhabditis elegans". free to MU campus, to others for purchase, 2000. http://wwwlib.umi.com/cr/mo/fullcit?p9988682.
Texto completoRicarte, Anânkia de Oliveira. "Herança da resistência do acesso AC-02 às raças 1 e 5 de Podosphaera xanthii em meloeiro". Universidade Federal Rural do Semi-Árido, 2016. http://bdtd.ufersa.edu.br:80/tede/handle/tede/620.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Powdery mildew is a disease that causes substantial losses in melon production around the world. Obtaining resistant cultivars is possible through introgression of alleles in the breeding program. In this study, it was investigated the inheritance of resistance of AC-02 accession to races 1 and 5 of Podosphaera xanthii in cross with susceptible cultivar ‘Védrantais’, under greenhouses conditions. The segregations ratios for resistance/susceptibility observed in the different populations (F1, F2, RC1 and RC2) indicated a monogenic and dominant inheritance in AC-02 to races 1 and 5. The distance between the gene controlling resistance to race 1 (px1-ac02) and the gene which confers resistance race 5 (px5-ac02) is 28.5 cM.
oídio é uma doença que causa perdas significativas na produção de melão em todo o mundo. A obtenção de cultivares resistentes é feita mediante introgressão de alelos de resistência. Neste estudo, investigou-se a herança da resistência do acesso AC-02 às raças 1 e 5 de Podosphaera xanthii por meio de cruzamento com a cultivar suscetível ‘Védrantais’, sob condições de casa de vegetação. As razões de segregações de resistência/suscetibilidade observadas nas diferentes populações (F1, F2, RC1 e RC2) indicaram que a herança da resistência do AC-02 às raças 1 e 5 é controlada, cada uma por um gene composto por dois alelos, de modo que o alelo que confere resistência domina o alelo para suscetibilidade. A distância entre o gene que controla a resistência à raça 1 (px1-ac02) e o gene que confere resistência à raça 5 (px5-ac02) é 28,5 cM.
2016-11-23
Nevils, Melissa A. "An analysis of factors related to virulence in babesia bovis /". free to MU campus, to others for purchase, 2001. http://wwwlib.umi.com/cr/mo/fullcit?p3025642.
Texto completoCASTI, ALBERTO. "Ruolo del trasportatore della serotonina in topi ipomorfici per il gene monoamino ossidasi A (MAO A): studio comportamentale e immunoistochimico". Doctoral thesis, Università degli Studi di Cagliari, 2011. http://hdl.handle.net/11584/265911.
Texto completoRobinson-Smith, Ruth A. "Interactions of MHC class I molecules with peptide ligands and [beta]₂-microglobulin". free to MU campus, to others for purchase, 1996. http://wwwlib.umi.com/cr/mo/fullcit?p9821331.
Texto completo