Bibliografías temáticas / Microsatellite loci

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Literatura académica sobre el tema "Microsatellite loci"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 24 de julio de 2025

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Artículos de revistas sobre el tema "Microsatellite loci"

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England, Phillip R., David A. Briscoe, and Richard Frankham. "Microsatellite polymorphisms in a wild population of Drosophila melanogaster." Genetical Research 67, no. 3 (1996): 285–90. http://dx.doi.org/10.1017/s0016672300033760.

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SummaryHighly variable DNA polymorphisms called microsatellites are rapidly becoming the marker of choice in population genetic studies. Until now, microsatellites have not been utilized for Drosophila studies. We have identified eight polymorphic microsatellite loci in Drosophila melanogaster and used them to characterize the genetic variation in a wild population from the Tyrrell's winery in Australia. Microsatellites were isolated from a partial genomic DNA library. All microsatellites consist of (AC)n repeats ranging from n = 2 to n = 24. Six loci were assigned to chromosomal location by g
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Yu, Kangfu, Soon J. Park, and Vaino Poysa. "Abundance and variation of microsatellite DNA sequences in beans (Phaseolus andVigna)." Genome 42, no. 1 (1999): 27–34. http://dx.doi.org/10.1139/g98-100.

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Microsatellites or simple sequence repeats (SSRs) have been demonstrated to be abundant and hypervariable in some eukaryotic genomes. Although the presence of microsatellites is very well documented in many plant species, no information on microsatellites in beans (Phaseolus andVigna) is available. To assess the abundance and usefulness of bean microsatellites as genetic markers, 326 DNA sequences from the GenBank databases were searched. Sixty-one simple repetitive DNA sequences with 23 different types of repetitive DNA motifs were identified as potential microsatellites. Among these were 49
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Hale, M. L., A. M. Borland, and K. Wolff. "High degree of conservation of nuclear microsatellite loci in the genus Clusia." Genome 48, no. 5 (2005): 946–50. http://dx.doi.org/10.1139/g05-048.

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In plants, microsatellites and their flanking DNA are rarely conserved across a whole genus, let alone other genera in the same family. Therefore, the possibility of using microsatellite primers developed for a species across a large number of plant species in the same genus is often limited. Remarkably, dinucleotide nuclear microsatellites developed for Clusia minor and for Clusia nemorosa amplified homologous microsatellites in species across the whole genus Clusia. In this present study, we report on the DNA sequence variation across the genus of 3 microsatellite loci with varying levels of
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Röder, Marion S., Victor Korzun, Bikram S. Gill, and Martin W. Ganal. "The physical mapping of microsatellite markers in wheat." Genome 41, no. 2 (1998): 278–83. http://dx.doi.org/10.1139/g98-009.

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Microsatellite markers represent a new class of genetic markers in plants. Such markers reveal a high level of polymorphism even in species with a narrow genetic base, such as hexaploid wheat (Triticum aestivum L.). We used a large set of such markers and 25 deletion stocks of 'Chinese Spring' in a deletion-mapping experiment to study the physical distribution of dinucleotide microsatellite markers in homoeologous group 2 chromosomes of hexaploid wheat. Thirty-one microsatellite markers identified 14 loci in chromosome 2A, 9 loci in chromosome 2B, and 10 loci in chromosome 2D. The microsatelli
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Colson, Isabelle, and David B. Goldstein. "Evidence for Complex Mutations at Microsatellite Loci in Drosophila." Genetics 152, no. 2 (1999): 617–27. http://dx.doi.org/10.1093/genetics/152.2.617.

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Abstract Fifteen lines each of Drosophila melanogaster, D. simulans, and D. sechellia were scored for 19 microsatellite loci. One to four alleles of each locus in each species were sequenced, and microsatellite variability was compared with sequence structure. Only 7 loci had their size variation among species consistent with the occurrence of strictly stepwise mutations in the repeat array, the others showing extensive variability in the flanking region compared to that within the microsatellite itself. Polymorphisms apparently resulting from complex nonstepwise mutations involving the micros
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Kushny, James E. E., John W. Coffin, and Curtis Strobeck. "Genetic survey of caribou populations using microsatellite DNA." Rangifer 16, no. 4 (1996): 351. http://dx.doi.org/10.7557/2.16.4.1277.

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Microsatellite loci are highly variable regions of eukaryotic DNA that consist of tandemly repeated sequences of one to six nucleotides in length. The use of microsatellites and the Polymerase Chain Reaction (PCR) are powerful tools for quantifying genetic variation within and among individual populations. Recently, we have developed primers for caribou that amplify 4 microsatellite loci. These microsatellite loci were used to survey the genetic variation in populations of Barren-ground caribou (Rangifer tarandus groenlandicus), Peary caribou (R.t. pearyi) and Woodland caribou (R.t. caribou) o
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Long, Dustin R., Adam Waalkes, Varun P. Panicker, Ronald J. Hause, and Stephen J. Salipante. "Identifying Optimal Loci for the Molecular Diagnosis of Microsatellite Instability." Clinical Chemistry 66, no. 10 (2020): 1310–18. http://dx.doi.org/10.1093/clinchem/hvaa177.

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Abstract Background Microsatellite instability (MSI) predicts oncological response to checkpoint blockade immunotherapies. Although microsatellite mutation is pathognomonic for the condition, loci have unequal diagnostic value for predicting MSI within and across cancer types. Methods To better inform molecular diagnosis of MSI, we examined 9438 tumor-normal exome pairs and 901 whole genome sequence pairs from 32 different cancer types and cataloged genome-wide microsatellite instability events. Using a statistical framework, we identified microsatellite mutations that were predictive of MSI w
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Gobily, Eman, Shimaa Kandel, Hadeer Auoda, Radwa Ahmed, and Mostafa Helal. "Whole-Genome Characterization and Analysis of Microsatellites in Turkey (Meleagris gallopavo) through in silico Approach." Biotecnia 27 (February 28, 2025): e2455. https://doi.org/10.18633/biotecnia.v27.2455.

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Turkey (Meleagris gallopavo) is the second largest contributor in poultry meat production after chickens. The study of microsatellite organization and distribution is of highly important in genomics and evolutionary students. The in silico mining for microsatellites leverages the power of computational biology to streamline and enhance the discovery of microsatellite markers, and reduce the cost of microsatellite detection. This study aimed at in silico mining microsatellite loci in the genome of domestic turkey. Reference sequences of the different chromosome obtained from NBCI and analyzed u
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Safaa, Hosam M., Mostafa Helal, Seif Yasser, et al. "Genome-Wide In Silico Analysis of Microsatellite Loci in Rabbits." Animals 14, no. 24 (2024): 3659. https://doi.org/10.3390/ani14243659.

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This study aimed to characterize microsatellites in the rabbit genome using an in silico approach and to develop and validate microsatellite markers. Blood samples were collected from 15 Baladi rabbits and 18 New Zealand White (NZW rabbits). The GMATA software was used to define SSRs in the extracted sequences. Twelve primer pairs were used to validate the loci identified and the primers developed. The total number of the detected microsatellite loci overall chromosomes was 1,136,253. The di-nucleotide microsatellite repeats dominated and exceeded 88% of the detected microsatellites in all chr
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McConnell, Stewart K., Patrick O'Reilly, Lorraine Hamilton, Jonathan M. Wright, and Paul Bentzen. "Polymorphic microsatellite loci from Atlantic salmon (Salmo salar): genetic differentiation of North American and European populations." Canadian Journal of Fisheries and Aquatic Sciences 52, no. 9 (1995): 1863–72. http://dx.doi.org/10.1139/f95-779.

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Atlantic salmon populations show low levels of genetic differentiation relative to other salmonid species, when surveyed by allozymes, and with mitochondrial DNA and nuclear ribosomal DNA markers. Here we report the application of three novel microsatellite VNTR loci to population differentiation in Atlantic salmon. A total of 232 microsatellites, cloned from Atlantic salmon, were classified as perfect, imperfect, and compound repeats. Microsatellite length, as in other teleosts, was significantly larger than published mammalian microsatellites. Primers for PCR amplification of three salmon mi
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Tesis sobre el tema "Microsatellite loci"

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Chilakamarri, Sunita R. "Genetic differentiation in Alewife populations using microsatellite loci." Link to electronic thesis, 2005. http://www.wpi.edu/Pubs/ETD/Available/etd-053105-164623/.

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Hey, Grace Valasi, University of Western Sydney, of Science Technology and Environment College, and of Science Food and Horticulture School. "Identification of individual koalas: microsatellite analysis of faecal DNA." THESIS_CSTE_SFH_Hey_G.xml, 2003. http://handle.uws.edu.au:8081/1959.7/451.

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Current studies of koalas in the wild mainly rely on information gathered by traditional field methods, such as community sightings, spotlighting, radiotracking, animal trappings, ear tagging and faecal pellet incidence. Collection of faeces is potentially the most reliable source of non-invasively obtaining DNA samples, which can be used to identify specific individuals. This thesis demonstrated a simple, rapid and reproducible method of extracting DNA from Koala faecal pellets using a commercially available DNA extraction kit, shows the maximum age of pellets from which DNA can be reliably e
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Field, Dawn. "Mutation, selection & the evolution of microsatellite loci in microbial genomes /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1998. http://wwwlib.umi.com/cr/ucsd/fullcit?p9911848.

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Dimsoski, Pero. "Variation in microsatellite loci and trait differences in Yorkshire and Large White /." The Ohio State University, 1996. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487935958845124.

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Henderson, Matt. "The effect of habitat fragmentation on the population genetic structure of the Western European hedgehog (Erinaceus europaeus)." Thesis, University of Southampton, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342656.

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Vishakha, FNU. "CHARACTERIZATION OF MICROSATELLITE LOCI AND PILOT POPULATION GENETIC ANALYSIS IN HICKORY SHAD, ALOSA MEDIOCRIS." VCU Scholars Compass, 2012. http://scholarscompass.vcu.edu/etd/422.

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The hickory shad (Alosa mediocris) is a relatively understudied species of the anadromous fish sub-family Alosinae. This study, the first population genetic analysis of this species, employed 12 neutral microsatellite loci to estimate genetic diversity and population structure in tributaries of lower Chesapeake Bay, Virginia including James River and its tributaries (Appomattox and Chickahominy Rivers), Rappahannock River, and Pamunkey River. Genetic variation was extremely low. Estimates of observed heterozygosity were lower than expected heterozygosity. Significant population structure wa
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Cunningham, Christopher. "Colerectal cancer genetics : a study of chromosome 8p tumour suppressor loci and microsatellite instability." Thesis, University of Edinburgh, 1997. http://hdl.handle.net/1842/21177.

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Fourteen chromosome 8 polymorphic loci were analysed for loss of heterozygosity in 119 colorectal cancers selected at random. Loss of heterozygosity was detected in 59.6% (59/99) of informative cases. Markers were of sufficient density to allow the construction of a deletion map which delineated two discrete regions likely to contain tumour suppressor loci. A 4cM region at 8p22-21.3 between markers D8S133 and LPL and a further locus at 8p21-11.2, between markers D8S137 and D8S136, estimated to span some 17cM. Loss of heterozygosity on chromosome 8p was found to be independent of tumour site, D
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Cloutier, Alison. "Assessment of size homoplasy at three microsatellite loci in the California market squid Loligo opalescens." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ61542.pdf.

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Paz-García, David A., Adrián Munguía-Vega, Tomas Plomozo-Lugo, and Amy Hudson Weaver. "Characterization of 32 microsatellite loci for the Pacific red snapper, Lutjanus peru, through next generation sequencing." SPRINGER, 2017. http://hdl.handle.net/10150/626030.

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We developed a set of hypervariable microsatellite markers for the Pacific red snapper (Lutjanus peru), an economically important marine fish for small-scale fisheries in the west coast of Mexico. We performed shotgun genome sequencing with the 454 XL titanium chemistry and used bioinformatic tools to search for perfect microsatellite loci. We selected 66 primer pairs that were synthesized and genotyped in an ABI PRISM 3730XL DNA sequencer in 32 individuals from the Gulf of California. We estimated levels of genetic diversity, deviations from linkage and Hardy-Weinberg equilibrium, estimated t
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Castilho, da Costa A. Rita C. F. "Genetic analysis of European seabass (Dicentrarchus Labrax L.) from Portuguese waters using allozyme and microsatellite loci." Thesis, University of Stirling, 1998. http://hdl.handle.net/1893/21585.

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Genetic differentiation among juvenile samples of the European seabass (Dicentrarchus labrax) from the coast of Portugal is reported by means of two types of genetic markers: allozymes and microsatellites. Repeat samples were taken from 5 different nursery grounds (Aveiro, Foz, Obidos, Milfontes and Faro) along the coast of Portugal between November 1992 and February 1994. Starch-gel electrophoresis was used to assess the level and distribution of genetic variability of 38 loci. Six of these were found to be polymorphic at the 99% level and were used in population surveys: AAT-3*, ADA *, GPI-I
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Capítulos de libros sobre el tema "Microsatellite loci"

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Flores-Rentería, Lluvia, and Andrew Krohn. "Scoring Microsatellite Loci." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-389-3_21.

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Andrés, José A., and Steven M. Bogdanowicz. "Isolating Microsatellite Loci: Looking Back, Looking Ahead." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-228-1_12.

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Rajora, O. P., and M. H. Rahman. "Microsatellite DNA markers and their usefulness in poplars, and conservation of microsatellite DNA loci in Salicaceae." In Genetic Response of Forest Systems to Changing Environmental Conditions. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9839-2_9.

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Stefanini, F. M., and M. W. Feldman. "Microsatellite Loci and the Origin of Modern Humans: A Bayesian Analysis." In Evolutionary Theory and Processes: Modern Perspectives. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4830-6_15.

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Chakraborty, Ranajit. "Statistical Issues Regarding the Use of Microsatellite Loci for Molecular Anthropological Studies." In Genomic Diversity. Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-4263-6_16.

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Fernandez-Silva, Iria, and Robert J. Toonen. "Optimizing Selection of Microsatellite Loci from 454 Pyrosequencing via Post-sequencing Bioinformatic Analyses." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-389-3_7.

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Schloitterer, Christian, and Thomas Wiehe. "Microsatellites, a neutral marker to infer selective sweeps." In Microsatellites: Evolution and Applications. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780198504085.003.0018.

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Abstract In recent years microsatellites have become popular as a highly variable neutral marker. In natural populations, microsatellite variability is determined by new mutations, genetic drift, and selection at linked chromosomal regions. In this chapter. we focus on the consequences of directional selection on observed microsatellite variability in natural populations. A test statistic is introduced that permits the identification of such 'selective sweeps'. The test is designed to detect reduced genetic variation in a specific locus-population combination from a data set of several microsa
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Eisen, Jonathan A. "Mechanistic basis for microsatellite instability." In Microsatellites: Evolution and Applications. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780198504085.003.0004.

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Abstract The inherent instability of microsatellite loci makes them exceptionally useful for evolutionary and genetic studies. This instability is predominantly due to changes in the number of copies of the microsatellite repeat. Most copy number changes at microsatellites are caused by slip-strand mispairing errors during DNA replication. Some of these errors are corrected by exonucleolytic proofreading and mismatch repair, but many escape repair and become mutations. Thus microsatellite instability can be considered to be a balance between the generation of replication errors by slip-strand
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Glenn, Travis C., and Nancy A. Schable. "Isolating Microsatellite DNA Loci." In Methods in Enzymology. Elsevier, 2005. http://dx.doi.org/10.1016/s0076-6879(05)95013-1.

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Stephens, J. Claiborne, Michael W. Smith, Hyoung Doo Shin, and Stephen J. O'Brien. "Tracking linkage disequilibrium in admixed populations with MALO using microsatellite loci." In Microsatellites: Evolution and Applications. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780198504085.003.0016.

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Abstract Microsatellite analyses have already had a profound impact on the construction of a human genetic linkage map, on the mapping of numerous disease gene loci, and on our understanding of human migration, demography, and evolution. In this report we summarize our recent efforts to extend microsatellite analysis to the mapping of loci involved in complex diseases. In particular, we focus on mapping by admixture linkage disequilibrium, dubbed MALD, which makes use both of the existing human microsatellite map and of human population genetic principles and data.
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Actas de conferencias sobre el tema "Microsatellite loci"

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Savichenko, Violetta, Svetlana Ramazanova, and Saida Guchetl. "Fragment analysis of microsatellite DNA loci for genotyping soybean varieties." In INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “CURRENT ISSUES OF BIOLOGY, BREEDING, TECHNOLOGY AND PROCESSING OF AGRICULTURAL CROPS” (CIBTA2022) (To the 110th anniversary of V.S. Pustovoit All-Russian Research Institute of Oil Crops). AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0140280.

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Юрьева, И. Б., И. С. Гавриличева, Н. В. Блохина, and Л. А. Храброва. "CHARACTERISTICS OF GENETIC STRUCTURE OF PECHORSKAYA HORSE ON MICROSATELLITE DNA LOCI." In СОВРЕМЕННЫЕ ДОСТИЖЕНИЯ И АКТУАЛЬНЫЕ ПРОБЛЕМЫ В КОНЕВОДСТВЕ. Crossref, 2019. http://dx.doi.org/10.25727/hs.2019.1.35400.

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В статье представлены результаты тестирования лошадей печорской породы по 17-ти панельным локусам микросателлитов ДНК. Проведенный генетико-популяционный анализ свидетельствует о уникальности генетической структуры печорской лошади, сохраняющий высокий уровень генетического разнообразия. The article presents the results of testing of Pechorskaya horses on 17 panel loci of DNA microsatellites. The conducted genetic and population analysis testifies to the uniqueness of the genetic structure of the Pechorskaya horse, preserving a high level of genetic diversity.
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Рамазанова, С. A., and В. Г. Савиченко. "THE ANALYSIS OF POLYMORPHISM OF DNA MICROSATELLITE LOCI FOR SOYBEAN GENOTYPING." In Материалы I Всероссийской научно-практической конференции с международным участием «Геномика и современные биотехнологии в размножении, селекции и сохранении растений». Crossref, 2020. http://dx.doi.org/10.47882/genbio.2020.93.48.023.

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Strange, James P. "Sixteen novel microsatellite loci forMegachile rotundata(Hymenoptera: Megachilidae) and related taxa." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.115455.

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Savichenko, V. G., and S. A. Ramazanova. "THE IDENTIFICATION OF SOYBEAN VARIETIES OF THE BREEDING OF V.S. PUSTOVOIT ALL-RUSSIAN RESEARCH INSTITUTE OF OIL CROPS BY MICROSATELLITE ANALYSIS." In 11-я Всероссийская конференция молодых учёных и специалистов «Актуальные вопросы биологии, селекции, технологии возделывания и переработки сельскохозяйственных культур». V.S. Pustovoit All-Russian Research Institute of Oil Crops, 2021. http://dx.doi.org/10.25230/conf11-2021-97-101.

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The identification of breeding material and certification of varieties is of great importance for the protection of the copyright of breeders. Microsatellite DNA loci are effectively used for these purposes. The aim of the research was to identify the soybean varieties of the breeding of V.S. Pustovoit All-Russian Research Institute of Oil Crops using the previously tested 12 microsatellite markers. As a result of research, we obtained the unique sets of alleles for eight varieties; two varieties had identical alleles. We divided all soybean genotypes into two large clusters. We observed the c
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Ilyasov, R. A., A. G. Nikolenko, and H. W. Kwon. "GENETIC IMPROVEMENT OF HONEY BEES FOR KEEPING IN EXTREMAL CLIMATIC CONDITIONS." In V International Scientific Conference CONCEPTUAL AND APPLIED ASPECTS OF INVERTEBRATE SCIENTIFIC RESEARCH AND BIOLOGICAL EDUCATION. Tomsk State University Press, 2020. http://dx.doi.org/10.17223/978-5-94621-931-0-2020-55.

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Genetic improvement of honey bee populations based on molecular genetics features is faster and precision in comparison with morphometry and behavior-based methods. We developed the method based on nine nuclear microsatellite loci that allow a selection of most adaptive honey bee colonies by genetically defined features. Our study the heterozygosity of the dark European bee A. m. mellifera inhabiting the extremely cold region of the Ural Mountains to provide a marker-assisted selection for revealing the high adapted to extremely cold climate honey bee population can be applied for markerassist
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A.V., Vorobieva, Golovinov I.V., Alimova A.Sh, Gaidamachenko V.N., and Nebesikhina N.A. "ASSESSMENT OF THE GENETIC DIVERSITY OF THE RUSSIAN STURGEON BROOD STOCK OF THE DONSKOY STURGEON PLANT BY MICROSATELLITE NUCLEAR MARKERS." In II INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE "DEVELOPMENT AND MODERN PROBLEMS OF AQUACULTURE" ("AQUACULTURE 2022" CONFERENCE). DSTU-Print, 2022. http://dx.doi.org/10.23947/aquaculture.2022.41-43.

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The article presents a study of the genetic diversity of the Russian sturgeon (Acipenser gueldenstaedtii) of the brood stock (RMS) of the Donskoy sturgeon hatchery (DOZ) using the analysis of microsatellite nuclear markers. In the course of the study, a general decrease in the heterozygosity of the population for four of the five loci studied was established, and in the study of the RMS population over the past two years, rare groups of alleles were identified that were not found in the sample for 2014. The data obtained can be used by the plant when drawing up crossbreeding schemes to preserv
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Zhongbao Li, Zhanlin Wang, Yuanyu Cao, et al. "Notice of Retraction: Isolation and characterization of five new polymorphic microsatellite loci of coelomactra antiquata (Spengler)." In 2010 2nd Conference on Environmental Science and Information Application Technology (ESIAT 2010). IEEE, 2010. http://dx.doi.org/10.1109/esiat.2010.5568534.

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Ramazanova, Svetlana, Evgeny Badyanov, and Saida Guchetl. "Identification and validation of microsatellite DNA loci of the downy mildew (Plarg) resistance gene in sunflower." In INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “CURRENT ISSUES OF BIOLOGY, BREEDING, TECHNOLOGY AND PROCESSING OF AGRICULTURAL CROPS” (CIBTA2022) (To the 110th anniversary of V.S. Pustovoit All-Russian Research Institute of Oil Crops). AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0140282.

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Шалаева, Т. В., and И. А. Шилов. "Promising Microsatellite Loci of the Sugarbeet (BETA VULGARIS) Genome for Genetic Analysis of Lines and Hybrids." In Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии. Crossref, 2022. http://dx.doi.org/10.48397/arriab.2022.22.xxii.031.

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Сахарная свёкла является важной технической культурой, на долю которой приходится примерно 40% мирового производства сахара. В настоящее время к числу важнейших хозяйственно-ценных требований к сорту, помимо выхода сахара с единицы сырья и площади посева, относятся повышение технологической пригодности сырья, получение семян с высокими посевными и физическими свойствами, толерантность к гербицидам, устойчивость к болезням, вредителям, факторам внешней среды, а главное – рентабельность производства в области семеноводства и возделывания коммерческих посевов сахарной свёклы. Sugar beet is an imp
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Informes sobre el tema "Microsatellite loci"

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ทัศนาขจร, อัญชลี. การศึกษาความแตกต่างแปรผันทางพันธุกรรมในประชากรกุ้งกุลาดำ โดยการตรวจลายพิมพ์ดีเอ็นเอ ด้วยตัวตรวจสอบที่มีลำดับเบสซ้ำแบบง่าย : รายงานวิจัยฉบับสมบูรณ์. จุฬาลงกรณ์์มหาวิทยาลัย, 1997. https://doi.org/10.58837/chula.res.1997.19.

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ในการศึกษาความแตกต่างแปรผันทางพันธุกรรมในประชากรกุ้งกุลาดำ ได้ทดสอบวิธีการตรวจลายพิมพ์ดีเอ็นเอในกุ้งกุลาดำและได้เลือก 2 วิธี เพื่อใช้ในการศึกษาความแตกต่างแปรผันทางพันธุกรรม คือ 1. การตรวจลายพิมพ์ดีเอ็นเอ โดยเทคนิค RAPD (Random Amplified Polymorphic DNA analysis) และ 2. การตรวจความแปรผันของ microsatellites DNA ในการศึกษานี้ได้เก็บตัวอย่างกุ้งจากแหล่งที่นิยมใช้เป็นพ่อ-แม่พันธุ์ในการเพาะเลี้ยงกุ้ง จำนวน 5 แหล่ง โดยเก็บตัวอย่างจากทะเลอันดามัน 3 แหล่ง ได้แก่ กุ้งบริเวณ จ. สตูล-ตรัง กุ้งจากจ. พังงา และกุ้งจากเมดาน ประเทศอินโดนีเซีย และเก็บตัวอย่างกุ้งจากฝั่งอ่าวไทย 2 แหล่ง ได้แก่ กุ้งจาก จ. ตราด และ
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Soller, Moshe (Morris), Hans Cheng, and Lyman Crittenden. Mapping the Chicken Genome, Including Loci Affecting Traits of Economic Importance. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568779.bard.

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A total of 195 microsatellites were added to the chicken genome map. Mapping of fifty known genes revealed a high degree of conserved linkage order between human and chicken genomes. A new, statistically powerful mapping design, the full-sib intercross line (produced by mating two parents, and intercrossing their progeny over a number of generations), was developed for use in species with high reproductive capacity. The Jerusalem Resource Population (JRP), now at the F12 generation, was established to implement this design i chickens. The biometrical picutre in the JRP is similar to that gener
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Sittipraneed, Siriporn. การศึกษาการแพร่กระจายของประชากรผึ้งโพรง Apis cerana กลุ่มตอนเหนือและตอนใต้ในบริเวณพื้นที่รอยต่อโดยใช้ดีเอ็นเอเครื่องหมาย : รายงานผลการวิจัย. Chulalongkorn University, 2002. https://doi.org/10.58837/chula.res.2002.30.

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PCR-RFLP of three mtDNA regions ( srRNA gene ,irRNA gene and intergenic COI – COII region) were used to investigated the distribution of northern and southern Apis cerana populations of 89 colonies from Prachuap Khiri Khan and Chumphon provinces. Three, four and eight haplotypes were obtained from DraI digestion of PCR-amplified 410 bp srRNA gene, 755bp IrRNA gene and 1710 bp intergenic COI-COII region,respectively. These three mtDNA regions generated 11 composit haplotypes. Twelve composite haplotypes were generated when samples from Yunnan and Hanoi were included. A UPGMA phenogram based on
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Gall, Graham A. E., Gideon Hulata, Eric M. Hallerman, Bernard May, and Umiel Nakdimon. Creating and Characterizing Genetic Variation in Tilapia through the Creation of an Artificial Center of Origin. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7574344.bard.

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Five stocks of tilapia [oreochromis niloticus (on), red O. niloticus (ROn), O. aureus (Oa), O. mossambicus (Om), and Sarotherodon galilaeus (Sg)] were used to produce two-way (F1), three-way (3WC) and four-way crosses (4WC). Three 4WC groups, containing equal representation of all four species, formed the base population for a new synthetic stock, called an "artificial center of origin" (ACO). Four genomic maps were created using microsatellite and AFLP markers, two from a 3WC family [Om female and (Oa x ROn) male] and two from a 4WC family [(Om x Oas) females and (Sg x On) male]. Sixty-two lo
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Veilleux, Richard E., Jossi Hillel, A. Raymond Miller, and David Levy. Molecular Analysis by SSR of Genes Associated with Alkaloid Synthesis in a Segregating Monoploid Potato Family. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7570550.bard.

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More than 15,000 anthers of an interspecific hybrid (CP2) between two diploid (2n=2x=24) potato species, Solanum chacoense (weedy) and S. phureja (cultivated), were cultured to generate a family of monoploid (haploid, 2n-1x=12) plants. Of 260 regenerated plants, 34 were monoploid, 210 diploid and 16 tetraploid. SSR analysis revealed that six monoploids were genetically identical and 14 diploids were homozygous, thus limiting the population to 42 (28 monoploids and 14 homozygous diploids). New microsatellite loci were developed for potato from database sequences (15), a conventional genomic lib
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Hulata, Gideon, and Graham A. E. Gall. Breed Improvement of Tilapia: Selective Breeding for Cold Tolerance and for Growth Rate in Fresh and Saline Water. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586478.bard.

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The main objective of this project was to initiate a breeding program to produce cold-tolerant and salinity-tolerant synthetic breeds of tilapia, from a base population consisting of a four-species hybrid population created under an earlier BARD project. A secondary objective was to estimate genetic parameters for the traits growth rate under fresh- and salt-water and for cold tolerance. A third objective was to place quantitative trait loci that affect these traits of interest (e.g., growth rate in fresh-water, salt-water and cold tolerance) on the growing linkage map of primarily microsatell
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Weller, Joel I., Harris A. Lewin, and Micha Ron. Determination of Allele Frequencies for Quantitative Trait Loci in Commercial Animal Populations. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7586473.bard.

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Individual loci affecting economic traits in dairy cattle (ETL) have been detected via linkage to genetic markers by application of the granddaughter design in the US population and the daughter design in the Israeli population. From these analyses it is not possible to determine allelic frequencies in the population at large, or whether the same alleles are segregating in different families. We proposed to answer this question by application of the "modified granddaughter design", in which granddaughters with a common maternal grandsire are both genotyped and analyzed for the economic traits.
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Weller, Joel, Harris Lewin, Micha Ron, and George Wiggans. Detection and Mapping of Genes Affecting Traits of Economic Importance in Dairy Cattle with the Aid of Molecular Genetic Markers. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613024.bard.

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Forty-seven poly-TG microsatellites were developed at the U of IL, and 11 genetic markers were developed at ARO, nine of which were poly-AGC microsatellites. Markers were typed on the reference families of CSIRO, Australia; GRANADA, Texas; and IRRF, Illinois, for chromosome assignment and linkage mapping. Nine North American al organizations contributed semen to the Dairy Bull DNA Repository (DBDR), which currently has 65,743 units from 3366 bulls. Semen was obtained for 31 out of 35 grandsires. Semen of 28 and 23 sons of two Israeli bulls was also collected. Eighteen grandsires were genotyped
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Weller, Joel, Harris Lewin, Micha Ron, George Wiggans, and Paul VanRaden. A Systematic Genome Search for Genes Affecting Economic Traits Dairy Cattle with the Aid of Genetic Markers. United States Department of Agriculture, 1999. http://dx.doi.org/10.32747/1999.7695836.bard.

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The objectives were to continue collection of semen for the US dairy bull DNA repository, to conduct a systematic search of the Holstein genome for economically significant economic trait loci (ETL), to develop and refine statistical techniques for the analysis of the data generated, and to confirm significant effects by genotyping daughters i Israel and additional US sons. One-thousand-seventy-six sons of eight US grandsires were genotyped for 174 microsatellites located on all 29 autosomes. ETL were detected for milk production traits on seven chromosomes. ETL for milk and fat yield and fat
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