Acceder

Bibliografías temáticas / Microdissection / Artículos de revistas

Siga este enlace para ver otros tipos de publicaciones sobre el tema: Microdissection.

Artículos de revistas sobre el tema "Microdissection"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 7 de julio de 2024

Crea una cita precisa en los estilos APA, MLA, Chicago, Harvard y otros

Elija tipo de fuente:

Consulte los 50 mejores artículos de revistas para su investigación sobre el tema "Microdissection".

Junto a cada fuente en la lista de referencias hay un botón "Agregar a la bibliografía". Pulsa este botón, y generaremos automáticamente la referencia bibliográfica para la obra elegida en el estilo de cita que necesites: APA, MLA, Harvard, Vancouver, Chicago, etc.

También puede descargar el texto completo de la publicación académica en formato pdf y leer en línea su resumen siempre que esté disponible en los metadatos.

Explore artículos de revistas sobre una amplia variedad de disciplinas y organice su bibliografía correctamente.

1

Qin, Dahui, Zhong Zheng, Shanxiang Shen, Prudence Smith y Farah K. Khalil. "Necessity of Microdissecting Different Tumor Components in Pulmonary Tumor Pyrosequencing". BioMed Research International 2016 (2016): 1–5. http://dx.doi.org/10.1155/2016/8759267.

Texto completo

Resumen

Microdissection is a useful method in tissue sampling prior to molecular testing. Tumor heterogeneity imposes new challenges for tissue sampling. Different microdissecting methods have been employed in face of such challenge. We improved our microdissection method by separately microdissecting the morphologically different tumor components. This improvement helped the pyrosequencing data analysis of two specimens. One specimen consisted of both adenocarcinoma and neuroendocrine components. When both tumor components were sequenced together for KRAS (Kirsten rat sarcoma viral oncogene homolog) gene mutations, the resulting pyrogram indicated that it was not a wild type, suggesting that it contained KRAS mutation. However, the pyrogram did not match any KRAS mutations and a conclusion could not be reached. After microdissecting and testing the adenocarcinoma and neuroendocrine components separately, it was found that the adenocarcinoma was positive for KRAS G12C mutation and the neuroendocrine component was positive for KRAS G12D mutation. The second specimen consisted of two morphologically different tumor nodules. When microdissected and sequenced separately, one nodule was positive for BRAF (v-raf murine sarcoma viral oncogene homolog B1) V600E and the other nodule was wild type at the BRAF codon 600. These examples demonstrate that it is necessary to microdissect morphologically different tumor components for pyrosequencing.

Los estilos APA, Harvard, Vancouver, ISO, etc.

2

Tangrea, Michael A., Rodrigo F. Chuaqui, John W. Gillespie, Mamoun Ahram, Gallya Gannot, Benjamin S. Wallis, Carolyn J. M. Best et al. "Expression Microdissection". Diagnostic Molecular Pathology 13, n.º 4 (diciembre de 2004): 207–12. http://dx.doi.org/10.1097/01.pdm.0000135964.31459.bb.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

3

Pixell, I. I. "Microdissection Laser". Biofutur 1999, n.º 193 (octubre de 1999): 52. http://dx.doi.org/10.1016/s0294-3506(00)87141-0.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

4

Hunt, Jennifer L. y Sydney D. Finkelstein. "Microdissection Techniques for Molecular Testing in Surgical Pathology". Archives of Pathology & Laboratory Medicine 128, n.º 12 (1 de diciembre de 2004): 1372–78. http://dx.doi.org/10.5858/2004-128-1372-mtfmti.

Texto completo

Resumen

Abstract Objective.—To describe the techniques for microdissection of paraffin-embedded and frozen tissue sections for the use in molecular applications. Data Sources.—Original research papers and review papers and the authors' personal experiences. Data Synthesis.—Manual and laser-capture microdissection are described in detail, with specific protocols for sample preparation and instructions for performing the microdissection. A section addressing frequently asked questions is also included. Conclusions.—Microdissection is a technique that is very useful both in the research setting and for clinical molecular testing in paraffin-embedded tissue samples. The available techniques range from simple and inexpensive (manual microdissection) to complex and expensive (laser-capture microdissection). All of the techniques, however, require the user to be familiar with microscopy and histology.

Los estilos APA, Harvard, Vancouver, ISO, etc.

5

Emmert-Buck, Michael R., Robert F. Bonner, Paul D. Smith, Rodrigo F. Chuaqui, Zhengping Zhuang, Seth R. Goldstein, Rhonda A. Weiss y Lance A. Liotta. "Laser Capture Microdissection". Science 274, n.º 5289 (8 de noviembre de 1996): 998–1001. http://dx.doi.org/10.1126/science.274.5289.998.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

6

Espina, Virginia, Julia D. Wulfkuhle, Valerie S. Calvert, Amy VanMeter, Weidong Zhou, George Coukos, David H. Geho, Emanuel F. Petricoin y Lance A. Liotta. "Laser-capture microdissection". Nature Protocols 1, n.º 2 (27 de junio de 2006): 586–603. http://dx.doi.org/10.1038/nprot.2006.85.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

7

Tresser, N., M. Ouezado, L. Whitney, K. Becker, R. Bonner, M. Emmert-Buck y L. Liotta. "LASER CAPTURE MICRODISSECTION". Journal of Neuropathology and Experimental Neurology 57, n.º 5 (mayo de 1998): 505. http://dx.doi.org/10.1097/00005072-199805000-00164.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

8

Jensen, Ellen. "Laser-Capture Microdissection". Anatomical Record 296, n.º 11 (4 de octubre de 2013): 1683–87. http://dx.doi.org/10.1002/ar.22791.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

9

Tsai, Cheng-Han, I.-Shen Huang, Wei-Jen Chen, Li-Hua Li, Eric Yi-Hsiu Huang y William J. Huang. "Repeat Microdissection Testicular Sperm Extraction in Azoospermic Men with Nonmosaic Klinefelter Syndrome". Andrologia 2023 (23 de mayo de 2023): 1–7. http://dx.doi.org/10.1155/2023/3955704.

Texto completo

Resumen

Introduction. To investigate the predictive factors for successful repeat microdissection testicular sperm extraction attempts in patients with Klinefelter syndrome. Methods. A total of 28 azoospermic men with nonmosaic Klinefelter syndrome who have received microdissection testicular sperm extraction twice with successful initial microdissection testicular sperm extraction attempts in our institute were studied. Outcome variables (age, serum follicle-stimulating hormone, luteinizing hormone, testosterone, prolactin, and estradiol) of azoospermic men with nonmosaic Klinefelter syndrome and a successful 2nd surgical sperm retrieval attempt (group A) were compared to those with an unsuccessful 2nd sperm retrieval attempt (group B). Results. Twenty-one patients (75%) had successful sperm recovery at the 2nd microdissection testicular sperm extraction attempt. The mean testosterone level at baseline and before the 1st microdissection testicular sperm extraction attempt was higher in group A than in group B (2.7 vs. 0.9 ng/mL, p < 0.01 , and 3.9 vs. 1.1 ng/mL, p = 0.02 ). Receiver operating characteristic curve analysis identified the threshold baseline testosterone concentration (1.5 ng/mL) of patients with Klinefelter syndrome in predicting successful 2nd sperm retrieval attempts and revealed positive and negative predictive values of 94.44% and 60%, respectively. Conclusion. Azoospermic men with Klinefelter syndrome presenting with low testosterone levels and successful sperm recovery during the first microdissection testicular sperm extraction procedure are unlikely to retrieve sperm on the 2nd microdissection testicular sperm extraction attempt. Hence, these patients should be properly counseled before sperm retrieval.

Los estilos APA, Harvard, Vancouver, ISO, etc.

10

Kumar, Pramod y Virendra Singh. "Modified microdissection electrocautery needle". National Journal of Maxillofacial Surgery 5, n.º 2 (2014): 243. http://dx.doi.org/10.4103/0975-5950.154849.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

11

Flannigan, Ryan, Phil V. Bach y Peter N. Schlegel. "Microdissection testicular sperm extraction". Translational Andrology and Urology 6, n.º 4 (agosto de 2017): 745–52. http://dx.doi.org/10.21037/tau.2017.07.07.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

12

Majer, Anna y Stephanie A. Booth. "Microdissection and transcriptional profiling". Prion 8, n.º 1 (enero de 2014): 67–74. http://dx.doi.org/10.4161/pri.27729.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

13

Espina, Virginia, Michael Heiby, Mariaelena Pierobon y Lance A. Liotta. "Laser capture microdissection technology". Expert Review of Molecular Diagnostics 7, n.º 5 (septiembre de 2007): 647–57. http://dx.doi.org/10.1586/14737159.7.5.647.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

14

Fend, Falko, Marcus Kremer, Katja Specht y Leticia Quintanilla-Martinez. "Laser Microdissection in Hematopathology". Pathology - Research and Practice 199, n.º 6 (enero de 2003): 425–30. http://dx.doi.org/10.1078/0344-0338-00441.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

15

Grant, Kenneth y W. Gray Jerome. "Laser Capture Microdissection as an Aid to Ultrastructural Analysis". Microscopy and Microanalysis 8, n.º 3 (junio de 2002): 170–75. http://dx.doi.org/10.1017/s143192760202010x.

Texto completo

Resumen

Laser capture microdissection uses a microscope to identify specific cells for microdissection and then a laser-sensitive plastic to capture and remove the cells from their substrate. This efficient capture method was originally developed to capture cells for genetic analysis. However, it has also been used to capture cells for proteonomic analysis. In this article, we extend the uses of laser-capture microdissection by reporting a method for preparing captured cells for ultrastructural analysis by transmission electron microscopy. Cells prepared by our methodology show good fine structure preservation and are easily sectioned by standard ultramicrotomy.

Los estilos APA, Harvard, Vancouver, ISO, etc.

16

Huang, Haibo, Yifan Pan, Yan Pang, Hao Shen, Xiwei Gao, Yichen Zhu, Liguo Chen y Lining Sun. "Piezoelectric Ultrasonic Biological Microdissection Device Based on a Novel Flexure Mechanism for Suppressing Vibration". Micromachines 12, n.º 2 (13 de febrero de 2021): 196. http://dx.doi.org/10.3390/mi12020196.

Texto completo

Resumen

Biological microdissection has a wide range of applications in the field of molecular pathology. The current laser-assisted dissection technology is expensive. As an economical microdissection method, piezoelectric ultrasonic microdissection has broad application prospects. However, the performance of the current piezoelectric ultrasonic microdissection technology is unsatisfactory. This paper aims to solve the problems of the low dissecting precision and excessive wear of the dissecting needle caused by the harmful lateral vibration of the present piezoelectric ultrasonic microdissection device. A piezoelectric ultrasonic microdissection device based on a novel flexure mechanism is proposed. By analyzing the flexure hinge flexibility, the type of flexure beam and the optimal design parameters are determined. Through harmonic response simulation analysis, the newly designed microdissection device with a vibration-suppressing mechanism achieves the best vibration effect when the driving frequency is 28 kHz. Under this driving frequency, the lateral vibration suppression effect is improved by 68% compared to the traditional effect without vibration suppression. Then, based on 3D printing technology, a prototype of a novel microdissection device is produced, and its performance is tested. Experiments on dissecting needle vibration tests show that the flexure mechanism does indeed suppress the lateral vibration of the needle tip. We conducted various tissue dissection experiments on paraffin tissue sections. First, we determine the optimal dissecting parameters (driving voltage, frequency, feed speed, cutting angle) of the new equipment through various parameter dissecting experiments. Then, we adopt these optimal dissecting parameters to perform three kinds of dissecting experiments on mouse tissue paraffin section (liver, lung, bone), dissecting experiments on tissue sections of different thicknesses (3 μm, 4 μm, 5 μm), sampling and extraction experiments on complete tissue. The new device has a better dissecting performance for paraffin tissue sections below a 5 μm thickness and can complete various dissecting tasks. Finally, we compare the wear of the dissecting needles of the new and old devices after the same dissecting tasks. The results prove that the suppression of harmful lateral vibration not only significantly improves the dissecting effect but also increases the service life and durability of the dissecting needle, which is beneficial for reducing the equipment costs.

Los estilos APA, Harvard, Vancouver, ISO, etc.

17

He, Wei, Yagang Liu, Moyra Smith y Michael W. Berns. "Laser Microdissection for Generation of a Human Chromosome Region-specific Library". Microscopy and Microanalysis 3, n.º 1 (enero de 1997): 47–52. http://dx.doi.org/10.1017/s1431927697970033.

Texto completo

Resumen

Abstract: A human chromosome pq34 region-specific, microdissected library was constructed by using laser microdissection techniques. This library contains over 10,000 clones with an average insert size of 450 bp. It has greater coverage of the dissected chromosome region as compared with the needle-dissected chromosome 9q34 library. The laser microdissection technique provides more accurate chromosome targeting and easier operation than existing needle microdissection techniques. To simplify the procedure for chromosome microdissection and chromosome fragment collection, a trapping-cutting system was developed. This technique involves the use of two trapping beams which hold a single chromosome in suspension, and a third cutting beam, which dissects the immobilized chromosome. A collection chamber allowing for the fast collection of dissected chromosome fragments needs to be developed. However, DNA can be cloned from trapped chromosome fragments with an insert size comparable to that of both needle-cut and laser-cut chromosomes.

Los estilos APA, Harvard, Vancouver, ISO, etc.

18

Bahary, Nathan, Donna E. McGraw, Rebecca Shilling y Jeffrey M. Friedman. "Microdissection and Microcloning of Mid-Chromosome 4: Genetic Mapping of 41 Microdissection Clones". Genomics 16, n.º 1 (abril de 1993): 113–22. http://dx.doi.org/10.1006/geno.1993.1148.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

19

Kim, Chong Jai. "Laser-based Microdissection of Cell". Journal of the Korean Medical Association 43, n.º 4 (2000): 353. http://dx.doi.org/10.5124/jkma.2000.43.4.353.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

20

Cannizzaro, L. A. "Chromosome microdissection: a brief overview". Cytogenetic and Genome Research 74, n.º 3 (1996): 157–60. http://dx.doi.org/10.1159/000134407.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

21

HaiJin, Yi, Guo Weiwei, Chen Lei, Wu Na, Li JiaNa, Ren LiLi y Yang ShiMing. "Microdissection of Miniature Pig Ear". Journal of Otology 8, n.º 2 (diciembre de 2013): 91–96. http://dx.doi.org/10.1016/s1672-2930(13)50019-5.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

22

Hudock, Teresa A., Andrew A. Lackner y Deepak Kaushal. "Microdissection approaches in tuberculosis research". Journal of Medical Primatology 43, n.º 5 (28 de agosto de 2014): 294–97. http://dx.doi.org/10.1111/jmp.12141.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

23

Chokechanachaisakul, Uraiwan, Tomoatsu Kaneko, Takashi Okiji, Reika Kaneko, Hideaki Suda y Jacques E. Nör. "Laser Capture Microdissection in Dentistry". International Journal of Dentistry 2010 (2010): 1–8. http://dx.doi.org/10.1155/2010/592694.

Texto completo

Resumen

Laser capture microdissection (LCM) allows for the microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. According to the recent development of the LCM technologies and methodologies, the LCM has been used in various kinds of tissue specimens in dental research. For example, the real-time polymerase-chain reaction (PCR) can be performed from the formaldehyde-fixed, paraffin-embedded, and immunostained sections. Thus, the advance of immuno-LCM method allows us to improve the validity of molecular biological analysis and to get more accurate diagnosis in pathological field in contrast to conventional LCM. This paper is focused on the presentation and discussion of the existing literature that covers the fields of RNA analysis following LCM in dentistry.

Los estilos APA, Harvard, Vancouver, ISO, etc.

24

Greulich, Karl Otto. "Chromosome microtechnology: microdissection and microcloning". Trends in Biotechnology 10 (1992): 48–51. http://dx.doi.org/10.1016/0167-7799(92)90168-u.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

25

Böhm, Christine, Dieter Newrzella y Oliver Sorgenfrei. "Laser microdissection in CNS research". Drug Discovery Today 10, n.º 17 (septiembre de 2005): 1167–74. http://dx.doi.org/10.1016/s1359-6446(05)03555-5.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

26

Gupta, S. K., A. G. Douglas-Jones y J. M. Morgan. "Microdissection of stained archival tissue." Molecular Pathology 50, n.º 4 (1 de agosto de 1997): 218–20. http://dx.doi.org/10.1136/mp.50.4.218.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

27

Fend, F. "Laser capture microdissection in pathology". Journal of Clinical Pathology 53, n.º 9 (1 de septiembre de 2000): 666–72. http://dx.doi.org/10.1136/jcp.53.9.666.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

28

NOGUCHI, MASAYUKI. "Tissue microdissection and molecular diagnosis". Juntendo Medical Journal 46, n.º 4 (2001): 416–22. http://dx.doi.org/10.14789/pjmj.46.416.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

29

Hsiao, W., R. Ramasamy, J. A. Ricci y P. N. Schlegel. "Microdissection TESE: the learning curve". Fertility and Sterility 94, n.º 4 (septiembre de 2010): S17—S18. http://dx.doi.org/10.1016/j.fertnstert.2010.07.068.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

30

Vila-Coro, Antonio Aguirre. "Vascular Microdissection in Strabismus Surgery". Archives of Ophthalmology 108, n.º 7 (1 de julio de 1990): 1034. http://dx.doi.org/10.1001/archopht.1990.01070090136056.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

31

Fend, Falko, Marcus Kremer y Leticia Quintanilla-Martinez. "Laser Capture Microdissection: Methodical Aspects and Applications with Emphasis on Immuno-Laser Capture Microdissection". Pathobiology 68, n.º 4-5 (2000): 209–14. http://dx.doi.org/10.1159/000055925.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

32

Zheng, Yi, Ding-Ming Li, Fu-Ping Li, Xiao-Hui Jiang, Luo Yang, Rui Qu, Heng-Zhou Bai, Gui-Cheng Zhao y Kun Tian. "Case report: remedial microdissection testicular sperm extraction after onco-microdissection testicular sperm extraction failure". Medicine 103, n.º 8 (23 de febrero de 2024): e37201. http://dx.doi.org/10.1097/md.0000000000037201.

Texto completo

Resumen

Background: Testicular cancer (TC) mostly occurs in men aged 14 to 44. Studies have shown that TC seriously damages male fertility, and 6% to 24% of patients with TC were even found to suffer from azoospermia when they are diagnosed. At present, some studies have pointed out that onco-microdissection testicular sperm extraction (mTESE) can extract sperm from tumor testicles. However, there are almost no reports on remedial measures after onco-mTESE failure. Given the valuable opportunity for fertility preservation in patients with TC and azoospermia, it is necessary to provide effective remedial methods for patients with failed onco-mTESE. Methods: Two young men, who were diagnosed with TC and also found to have azoospermia, tried onco-mTESE while undergoing radical orchiectomy for fertility preservation. However, sperm extraction failed in both patients. Subsequently, the isolated testicular tissue of the patient in case 1 suffered from TC again, and the patient in case 2 was scheduled to receive multiple cycles of gonadotoxic chemotherapy. Because both had a plan to have a birth in the future, we performed remedial mTESE. Results: Sperm was successfully extracted from both patients. The patient recovered well, without complications. The patient couple in case 1 underwent 1 intracytoplasmic sperm injection (ICSI) cycle but did not achieve clinical pregnancy. Conclusions: There is still an opportunity to extract sperm successfully using onco-mTESE, despite the difficulty of fertility preservation in TC patients with azoospermia. If sperm extraction from the tumor testis fails, implementing remedial mTESE as early as possible would likely preserve the last chance of fertility for these patients.

Los estilos APA, Harvard, Vancouver, ISO, etc.

33

Hsiao, Wayland, Peter J. Stahl, E. Charles Osterberg, Edward Nejat, Gianpiero D. Palermo, Zev Rosenwaks y Peter N. Schlegel. "Successful Treatment of Postchemotherapy Azoospermia With Microsurgical Testicular Sperm Extraction: The Weill Cornell Experience". Journal of Clinical Oncology 29, n.º 12 (20 de abril de 2011): 1607–11. http://dx.doi.org/10.1200/jco.2010.33.7808.

Texto completo

Resumen

Purpose Advances in chemotherapy have led to greater longevity and paternity may be an important consideration for postchemotherapy survivors of childhood cancers. While traditionally considered sterile, men who are azoospermic after chemotherapy can be treated with microdissection testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI). Patients and Methods Oncologic data, pretreatment hormone profiles, testicular histology, and outcomes of microdissection TESE-ICSI were reviewed. ICSI was performed in a programmed in vitro fertilization cycle using fresh spermatozoa. Embryos were transferred into the uterine cavity on the third day after microinjection. Results Eighty-four microdissection TESE procedures were performed in 73 patients. The mean time elapsed since chemotherapy was 18.6 years (range, 1 to 34 years). Spermatozoa were retrieved in 37% of patients and in 42.9% of overall procedures. A 57.1% fertilization rate (per injected oocyte) was achieved with ICSI allowing a 50% clinical pregnancy rate with a live birth rate of 42% overall. There were 15 deliveries, with a total of 20 children born. Hypospermatogenesis seen on preoperative biopsy was associated with 100% sperm retrieval while exposure to alkylating agents resulted in a significantly lower sperm retrieval rate. Patients with testicular cancer had the highest sperm retrieval rates while patients previously treated for sarcoma had the lowest retrieval rates. Conclusion To our knowledge, this represents the largest series of postchemotherapy microdissection TESE-ICSI to date. Sperm were retrieved in 37% of patients despite a prevalence of Sertoli cell–only pattern on preoperative biopsy. Although prechemotherapy sperm cryopreservation is recommended, treatment with microdissection TESE and ICSI are effective treatment options for many azoospermic men after chemotherapy.

Los estilos APA, Harvard, Vancouver, ISO, etc.

34

Llambí, S. y M. V. Arruga. "Molecular approach of the fragile chromosomal region Xq31-34 in cattle (Bos taurus) by microdissection and DOP-PCR". Arquivo Brasileiro de Medicina Veterinária e Zootecnia 60, n.º 4 (agosto de 2008): 926–31. http://dx.doi.org/10.1590/s0102-09352008000400023.

Texto completo

Resumen

Fragile sites (FS) are chromosomal regions where the normal compactation of chromatine is not observed. FRAXA (Fra Xq27.3, X sexual chromosome) is one of the most studied FS in humans. FRAXA is an expansion of the trinucleotide CGG located in the gene FMR-1. In cattle, sites of chromosomal fragility were reported in BTAX, associated with different pathologies and fertility impairment. Chromosomal microdissection has became a valuable tool for isolating chromatine fragments. In this work, it was combined the chromosomal microdissection technique with DOP-PCR in order to carry out a molecular analysis of the fragile chromosomal region BTAXq31-34. In that region, polymorphic DNA-RAPD sequences (GC rich) are present and sequences of the gene FMR-1 are missing. The results showed the usefulness of the microdissection-DOP-PCR technique for molecular characterization of fragile chromosomal sites in cattle.

Los estilos APA, Harvard, Vancouver, ISO, etc.

35

Wong, Chui E., Mohan B. Singh y Prem L. Bhalla. "Sample preparation for laser-microdissection of soybean shoot apical meristem". International Journal of Plant Biology 3, n.º 1 (16 de octubre de 2012): 3. http://dx.doi.org/10.4081/pb.2012.e3.

Texto completo

Resumen

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next generation sequencing permits an in-depth analysis of molecular events associated with specific cell type of interest. Sample preparation is the most critical step in ensuring good quality RNA to be extracted from samples following laser-microdissection. Here, we optimized the sample preparation for a major legume crop, soybean. We used Farmer’s solution as a fixative and paraffin as the embedding medium for soybean shoot apical meristem tissue without the use of any specialized equipment. Shorter time for tissue fixation (two days) was found to be critical for the preservation of RNA in soybean shoot apical meristem. We further demonstrated the utility of this method for different tissues derived from soybean and rice. The method outlined here shall facilitate studies on crop plants involving laser-microdissection.

Los estilos APA, Harvard, Vancouver, ISO, etc.

36

Dahse, Regine y Hartwig Kosmehl. "CELL SEPARATION AND GENE EXPRESSION ANALYSIS IN A TUMOR-STROMA INTERACTION MODEL". Image Analysis & Stereology 23, n.º 3 (3 de mayo de 2011): 153. http://dx.doi.org/10.5566/ias.v23.p153-157.

Texto completo

Resumen

A novel technique for co-culturing and separating fibroblasts and carcinoma cells in a 2-D model of tumorstroma interaction is presented. The methodology is based on cell co-cultivation on an 1.35 μm thin membrane followed by rapid immunostaining and microdissection of the different cell compartments using a laser microdissection system (P.A.L.M. Microlaser Technologies AG, Germany). For identifying the tumor cell compartment, immunolabeling for a marker that is expressed only in epithelial tumor cells is performed. The RNA quality from the microdissected co-cultured cells was successfully proved by RT-PCR for a housekeeping gene transcript and for the laminin gamma 2 chain gene transcript used before in the tumor cell immunostaining. Laminin cDNA was amplificable only in tumor cells and not in the co-cultivated fibroblasts indicating no cell-cross-contamination during microdissection. Microdissected tumor and stroma cells from the presented membrane based co-culture model can be used for gene expression profiling and DNA based analysis in the investigation of tumor-stroma interactions.

Los estilos APA, Harvard, Vancouver, ISO, etc.

37

Nambiar, P. R., S. R. Boutin, R. Raja y D. W. Rosenberg. "Global Gene Expression Profiling: A Complement to Conventional Histopathologic Analysis of Neoplasia". Veterinary Pathology 42, n.º 6 (noviembre de 2005): 735–52. http://dx.doi.org/10.1354/vp.42-6-735.

Texto completo

Resumen

Transcriptional profiling of entire tumors has yielded considerable insight into the molecular mechanisms of heterogeneous cell populations within different types of neoplasms. The data thus acquired can be further refined by microdissection methods that enable the analyses of subpopulations of neoplastic cells. Separation of the various components of a neoplasm (i.e., stromal cells, inflammatory infiltrates, and blood vessels) has been problematic, primarily because of a paucity of tools for accurate microdissection. The advent of laser capture microdissection combined with powerful tools of linear amplification of RNA and high-throughput microarray-based assays have allowed the transcriptional mapping of intricate and highly complex networks within pure populations of neoplastic cells. With this approach, specific “molecular signatures” can be assigned to tumors of distinct or even similar histomorphology, thereby aiding the desired objective of pattern recognition, tumor classification, and prognostication. This review highlights the potential benefits of global gene expression profiling of tumor cells as a complement to conventional histopathologic analyses.

Los estilos APA, Harvard, Vancouver, ISO, etc.

38

ZHOU, JI-BO, SHENGFANG GE, PING GU, DUO PENG, GUO-FU CHEN, MIAO-ZHEN PAN y JIA QU. "Microdissection of guinea pig extraocular muscles". Experimental and Therapeutic Medicine 2, n.º 6 (2011): 1183–85. http://dx.doi.org/10.3892/etm.2011.341.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

39

Sujatha, Govindarajan y Jayanandan Muruganandhan. "Laser Capture Microdissection in Oral Cancer". Journal of Contemporary Dental Practice 19, n.º 5 (2018): 475–76. http://dx.doi.org/10.5005/jp-journals-10024-2286.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

40

Wright, Charles G. y William L. Meyerhoff. "Microdissection in Human Temporal Bone Morphology". Annals of Otology, Rhinology & Laryngology 98, n.º 12_suppl (diciembre de 1989): 25–28. http://dx.doi.org/10.1177/0003489489098s1213.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

41

Lechpammer, Mirna. "LASER CAPTURE MICRODISSECTION: METHODS AND PROTOCOLS". Shock 25, n.º 4 (abril de 2006): 426–27. http://dx.doi.org/10.1097/01.shk.0000223839.51394.1c.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

42

Goldstein, Seth R., Philip G. McQueen y Robert F. Bonner. "Thermal modeling of Laser Capture Microdissection". Applied Optics 37, n.º 31 (1 de noviembre de 1998): 7378. http://dx.doi.org/10.1364/ao.37.007378.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

43

Brooks, Lionel, Josh Strable, Xiaolan Zhang, Kazuhiro Ohtsu, Ruilian Zhou, Ananda Sarkar, Sarah Hargreaves et al. "Microdissection of Shoot Meristem Functional Domains". PLoS Genetics 5, n.º 5 (8 de mayo de 2009): e1000476. http://dx.doi.org/10.1371/journal.pgen.1000476.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

44

Métézeau, Philippe y Mustapha Bensaasa. "La microdissection de chromosomes par laser". Biofutur 1997, n.º 165 (marzo de 1997): 13A. http://dx.doi.org/10.1016/s0294-3506(97)87634-x.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

45

Murray, Graeme I. "An overview of laser microdissection technologies". Acta Histochemica 109, n.º 3 (junio de 2007): 171–76. http://dx.doi.org/10.1016/j.acthis.2007.02.001.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

46

Santegoeds, R. G. C., Y. Yakkioui, A. Jahanshahi, G. Raven, J. J. Van Overbeeke, A. Herrler y Y. Temel. "Notochord isolation using laser capture microdissection". Journal of Chemical Neuroanatomy 80 (marzo de 2017): 37–43. http://dx.doi.org/10.1016/j.jchemneu.2016.12.004.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

47

Samuel, Raheel, Odgerel Badamjav, Kristin E. Murphy, Darshan P. Patel, Jiyoung Son, Bruce K. Gale, Douglas T. Carrell y James M. Hotaling. "Microfluidics: The future of microdissection TESE?" Systems Biology in Reproductive Medicine 62, n.º 3 (22 de abril de 2016): 161–70. http://dx.doi.org/10.3109/19396368.2016.1159748.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

48

Dabaja, Ali A. y Peter N. Schlegel. "Microdissection testicular sperm extraction: an update". Asian Journal of Andrology 15, n.º 1 (17 de diciembre de 2012): 35–39. http://dx.doi.org/10.1038/aja.2012.141.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

49

Fominaya, A., C. Linares, Y. Loarce y E. Ferrer. "Microdissection and microcloning of plant chromosomes". Cytogenetic and Genome Research 109, n.º 1-3 (2005): 8–14. http://dx.doi.org/10.1159/000082376.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

50

Chimenti, Cristina, Maurizio Pieroni, Andrea Russo, Patrizio Sale, Matteo A. Russo, Attilio Maseri y Andrea Frustaci. "Laser Microdissection in Clinical Cardiovascular Research". Chest 128, n.º 4 (octubre de 2005): 2876–81. http://dx.doi.org/10.1378/chest.128.4.2876.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Ofrecemos descuentos en todos los planes premium para autores cuyas obras están incluidas en selecciones literarias temáticas. ¡Contáctenos para obtener un código promocional único!