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Artículos de revistas sobre el tema "Massaria"

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Autor: Grafiati

Publicado: 1 de abril de 2023

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1

Chlebicki, Andrzej. "Some ascomycete fungi from primeval forests of north-eastern Poland". Acta Mycologica 40, n.º 1 (20 de agosto de 2014): 71–94. http://dx.doi.org/10.5586/am.2005.008.

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There are presented localities of 100 ascomycetc spccics collected in primeval forest of north-eastern part of Poland. Among them some new species for Poland were collected: <i>Eutypa lata var. aceri, Hypoderma sarmentorum, Lophiotrema curreyi, Massaria sorbi, Massarina chamaecyparisii, Mollisia poaeoides, Mycosphaerella lycopodii-annotini, Phaeosphaeria juncina, Ph. phragmilicola, Plagiosphaera immersa</i>.

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2

SAMARAKOON, MILAN C., JIAN-KUI LIU, SAMANTHA C. KARUNARATHNA, NILITA MUKJANG y RATCHADAWAN CHEEWANGKOON. "Massaria broussonetiae sp. nov. and M. racemosae sp. nov. (Massariaceae, Pleosporales) on moraceous hosts". Phytotaxa 559, n.º 3 (25 de agosto de 2022): 263–74. http://dx.doi.org/10.11646/phytotaxa.559.3.4.

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Massaria species are well-known in temperate regions in contrast to tropical regions. Taxonomy and phylogeny of two new Massaria species are carried out in this study as a part of our major research interests in microfungal diversity and distribution in Asia. In 2018–2019, we collected two fungal collections similar to Massaria from China and Thailand. Based on distinct morphology, multigene phylogeny, and host data, we introduce two new species, Massaria broussonetiae on Broussonetia sp. and M. racemosae on Ficus racemosa. Full descriptions, colour photo plates and a phylogenetic tree to show the placement of new species are provided.

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3

Schmitt, Uwe, Benjamin Lüer, Dirk Dujesiefken y Gerald Koch. "The Massaria disease of plane trees:its wood decay mechanism". IAWA Journal 35, n.º 4 (6 de diciembre de 2014): 395–406. http://dx.doi.org/10.1163/22941932-00000074.

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Branches of Platanus × hispanica with distinct symptoms of the Massaria disease were investigated by light and transmission electron microscopy and cellular UVmicrospectrophotometry. The samples collected in the city of Mannheim, Germany, were infected in vivo with the fungus Splanchnonema platani and showed various degrees of wood decay. The investigations were focused on the decay pattern of cell walls in the different cells, i.e., fibres, vessels as well as ray and axial parenchyma cells. The following results were obtained. Hyphae of the ascomycete fungus Splanchnonema platani penetrated from cell to cell through the pits and not through the cell wall middle lamella, by the formation of thin perforation hyphae. During this process, the 1–5 μm thick hyphae became narrower without attacking the wall around the pit canal. After penetration through a pit, the hyphae again enlarged to their original diameter. This is true for all pit pairs connecting the various cell types. Late decay stages did not show a decay of cell corner regions and middle lamellae of fibres as well as vessel and parenchyma cell walls. Phenolic deposits in parenchyma cells were still present in severely attacked xylem tissue. These features point to a low lignolytic capacity of the fungus. The frequently found microscopic decay pattern with the formation of oval or spherical cavities in the S2 layer of the secondary wall with an often structurally intact S3 layer is a characteristic of softrot decay. This classification is also supported by the remaining cell corner and middle lamella regions in advanced decay stages. As a consequence of this decay type, branches fracture in a brittle mode.

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4

HUANRALUEK, NARUEMON, KASUN M. THAMBUGALA, YONG WANG y KEVIN D. HYDE. "Introducing Massarioramusculicola, a novel genus in Massariaceae". Phytotaxa 371, n.º 1 (25 de septiembre de 2018): 17. http://dx.doi.org/10.11646/phytotaxa.371.1.2.

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The species of the family Massariaceae have been reported as hemibiotrophs, saprotrophs or weak pathogens. Massarioramusculicols chiangraiensis gen. et sp. nov. (Massariaceae) is introduced in this study to accommodate a taxon isolated from dead twigs of an unknown host, collected in Thailand. Phylogenetic analysis of large subunit (LSU) rRNA sequence data and morphological studies show the relationships of M. chiangraiensis with other genera in Massariaceae. Three genera Massaria, Massarioramusculicola and Neomassaria are accepted in Massariaceae and a key to genera of the family is provided. The genus Massarioramusculicola is clearly different from Massaria, whereas the latter has larger ascomata typically firmly embedded in pseudostromatic tissues intermixed with substrate cells, often surrounded by blackened marginal zones and covered by a dark clypeus, as well as brown to dark brown, larger, 3-disto- or euseptate ascospores. Massarioramusculicola can be distinguished from Neomassaria in having a peridium with two strata comprising dark brown to hyaline cells of textura angularis, rarely branched, septate trabeculae and 3-septate ascoapores.

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5

Sabovljevic, Marko, Gordana Tomovic, Jovana Pantovic, Sanja Djurovic, Uros Buzurovic, Teodor Denchev, Cvetomir Denchev et al. "New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 9". Botanica Serbica 46, n.º 2 (2022): 311–20. http://dx.doi.org/10.2298/botserb2202311s.

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This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: red algae Lemanea fucina and Paralemanea annulata, parasitic fungus Anthracoidea pratensis, saprotrophic fungi Cyathus olla, Massaria campestris, and Xylaria sicula, stonewort Chara canescens, liverworts Gymnomitrion commutatum and Porella baueri, moss Acaulon triquetrum, monocots Anacamptis laxiflora, Cephalanthera damasonium, and Himantoglossum robertianum and dicot Jacobaea othonnae are given within SE Europe and adjacent regions.

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6

Voglmayr, Hermann y Walter M. Jaklitsch. "Molecular data reveal high host specificity in the phylogenetically isolated genus Massaria (Ascomycota, Massariaceae)". Fungal Diversity 46, n.º 1 (enero de 2011): 133–70. http://dx.doi.org/10.1007/s13225-010-0078-5.

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7

Khvalkov, Evgeny A. "Europeans in the Black Sea Area during the Late Middle Ages: The Genoese Colony of Caffa". Mediaevistik 31, n.º 1 (1 de enero de 2018): 213–34. http://dx.doi.org/10.3726/med.2018.1.11.

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The four brief papers following below continue the line of reports on the archival finds of the Western migrants to the Genoese overseas colony of Caffa from outside Liguria in the fourteenth and fifteenth centuries, previously published in Archivio Storico Messinese,1 Rassegna Storica Salernitana,2 Studi Piemontesi,3 Studi veneziani,4 and Atti della Società Ligure di Storia Patria.5 The main sources researched for these studies are Caffae Massariae – the public books of accounts of the treasury of the Commune of Caffa drawn by the officers called massarii. These officers were annually rotated and sent from metropolis (Genoa) to its colony (Caffa). Caffae Massariae reflect money transactions and operations of the treasury in the double-entry bookkeeping system. The sources are stored in the archival section of the Bank of Saint George.6 Since Caffae Massariae quote (directly or indirectly) all those city inhabitants, who did with the administration any kind of financial transaction, they reflect the main flows of Latin migration from the West to the overseas Eastern colonies.

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8

Talakh, Victor N. "Genoese-Tatar Treaties of 1380 and 1381 in Their Historical Context". Golden Horde Review 9, n.º 1 (29 de marzo de 2021): 108–48. http://dx.doi.org/10.22378/2313-6197.2021-9-1.108-148.

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Research objective: An analysis of the provisions and circumstances of the conclusion of the Genoese-Tatar treaties of November 1380 and February 1381. Research materials: Political, legal, and economic clauses of the agreements of 1380–1381, the available information on the persons who participated in their signing, the circumstances of their conclusion. Results and novelty of the research: Although the content of both documents is identical, and they differ only in the groups of people who signed them on the Horde’s side, the author argues here that these are two independent acts. According to the author, both agreements were concluded on behalf of and in the interests of Toqtamïsh Khan. Based on numismatic data, the dating of the yarliq to Bek Haji, and records in the book of Caffa’s Massaria, the author is inclined to an early dating of the establishment of Toqtamïsh Khan’ power in Crimea, namely, before March 1380. The author is of the opinion that the existence of two agreements was in connection with a change of administration in Solkhat (although the details of this are unclear). He also considers highly plausible N. Murzakevich’s assumption that the cession to the Genoese of Soldaia district and Crimean Gothia, stipulated by treaties, was a reward for the murder of Mamai which occurred perhaps between 28 November 1380 and 23 January 1381.

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9

Zimmermann, K. "PYTHEAS OF MASSALIA". Classical Review 50, n.º 1 (abril de 2000): 28–30. http://dx.doi.org/10.1093/cr/50.1.28.

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10

Aptroot, André. "A world revision of Massarina (Ascomycota)". Nova Hedwigia 66, n.º 1-2 (13 de marzo de 1998): 89–162. http://dx.doi.org/10.1127/nova.hedwigia/66/1998/89.

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11

Hyde, Kevin D. "The genus Massarina, with a description of M. eburnea and an annotated list of Massarina names". Mycological Research 99, n.º 3 (marzo de 1995): 291–96. http://dx.doi.org/10.1016/s0953-7562(09)80902-7.

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12

Chevillon, Jean-Albert. "La Phase Postarchaïque du monnayage de Massalia". Revue numismatique 6, n.º 168 (2012): 135–58. http://dx.doi.org/10.3406/numi.2012.3178.

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13

Tanaka, Kazuaki y Yukio Harada. "Pleosporales in Japan (3). The genus Massarina". Mycoscience 44, n.º 3 (junio de 2003): 173–85. http://dx.doi.org/10.1007/s10267-003-0102-7.

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14

Shearer, C. A. y K. D. Hyde. "Massarina ingoldiana, a New Ascomycete from Freshwater Habitats". Mycologia 89, n.º 1 (enero de 1997): 114. http://dx.doi.org/10.2307/3761180.

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15

Tsui, K. M., S. W. Wong, K. D. Hyde y I. J. Hodgkiss. "Ultrastructural Studies of Massarina ingoldiana and M. purpurascens". Mycologia 91, n.º 5 (septiembre de 1999): 721. http://dx.doi.org/10.2307/3761524.

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16

Shearer, C. A. y K. D. Hyde. "Massarina ingoldiana, a new ascomycete from freshwater habitats". Mycologia 89, n.º 1 (enero de 1997): 114–19. http://dx.doi.org/10.1080/00275514.1997.12026762.

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17

Tsui, K. M., S. W. Wong, K. D. Hyde y I. J. Hodgkiss. "Ultrastructural studies of Massarina ingoldiana and M. purpurascens". Mycologia 91, n.º 5 (1 de septiembre de 1999): 721–26. http://dx.doi.org/10.1080/00275514.1999.12061076.

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18

Huhndorf, Sabine M. y Richard C. Harris. "Oletheriostrigula, a New Genus for Massarina papulosa (Fungi, Ascomycetes)". Brittonia 48, n.º 4 (octubre de 1996): 551. http://dx.doi.org/10.2307/2807875.

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19

Rosello, Mireille. "De Massalia à la Planète Mars: Métissage ou "fréquentage"". L'Esprit Créateur 41, n.º 3 (2001): 24–36. http://dx.doi.org/10.1353/esp.2010.0208.

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20

Scheuer, Ch. "Massarina tetraploa sp. nov., the teleomorph of Tetraploa aristata". Mycological Research 95, n.º 1 (enero de 1991): 126–28. http://dx.doi.org/10.1016/s0953-7562(09)81370-1.

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21

Chethana, Thilini, Mei Liu, Hiran A. Ariyawansa, Sirinapa Konta, Dhanushka N. Wanasinghe, Ying Zhou, Jiye Yan et al. "Splanchnonema-like species in Pleosporales: introducing Pseudosplanchnonema gen. nov. in Massarinaceae". Phytotaxa 231, n.º 2 (20 de octubre de 2015): 133. http://dx.doi.org/10.11646/phytotaxa.231.2.2.

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In this paper we introduce a new genus Pseudosplanchnonema with P. phorcioides comb. nov., isolated from dead branches of Acer campestre and Morus species. The new genus is confirmed based on morphology and phylogenetic analyses of sequence data. Phylogenetic analyses based on combined LSU and SSU sequence data showed that P. phorcioides formed a distinct clade within the family Massarinaceae and is sister to Massarina eburnea, the type species of Massarina. The new genus Pseudosplanchnonema differs from Massarina in having ascomata without clypei, a thick peridium and larger, 1-septate, guttulate, dark brown ascospores. The new genus is compared with genera in the family Massarinaceae and a detailed description and illustrations of the species P. phorcioides, including its asexual morph, is provided.

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22

Luo, Chunqing, Libin Deng, Changqing Zeng y You-Xin Jin. "High Throughput SNP Genotyping with Two Mini-sequencing Assays". Acta Biochimica et Biophysica Sinica 36, n.º 6 (1 de junio de 2004): 379–84. http://dx.doi.org/10.1093/abbs/36.6.379.

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Abstract Two mini-sequencing methods, FP-TDI (template-directed dye-terminator incorporation with fluorescence-polarization) and MassArray (matrix assisted laser desorption ionization time of flight detection mass spectrometry), were optimized. A numeric standard was introduced to evaluate the SNP scoring quality of FP-TDI assay, thus made the optimization work easier. At the same time, using multi-PCR technology, 8-plex genotyping of MassArray assay was successfully carried out, some softwares were developed and the data process of MassArray was highly automated. Then these two methods were applied to high throughput SNP genotyping, the accuracy, efficiency and robustness were compared. The result shows FP-TDI is more sensitive to the concentration of SNPprimer and PCR product, as well as extension cycles, the SNPprimer length of FP-TDI should be 24–30 bp long, whereas MassArray assay prefers to be as short as only 16 bp. Altogether 6440 SNP sites of human chromosome 3 were genotyped in a sample of 90 individuals, 4792 sites by FP-TDI assay and 1648 sites by MassArray assay, the success rates of FP-TDI and MassArray were 67.7% and 93.6% respectively. The throughput of MassArray was higher than FP-TDI, and the cost of MassArray was lower, MassArray was more suitable for high throughput SNP genotyping.

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23

Aptroot, André, Jane Fröhlich y Kevin D. Hyde. "Fungi from palms. XLIV. Two new Massarina species with pigmented ostioles". Nova Hedwigia 70, n.º 1-2 (1 de febrero de 2000): 227–32. http://dx.doi.org/10.1127/nova.hedwigia/70/2000/227.

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24

Hyde, Kevin D. y André Aptroot. "Fungi from palms. XXXIII. The genus Massarina, with a new species". Nova Hedwigia 64, n.º 3-4 (22 de septiembre de 1997): 491–504. http://dx.doi.org/10.1127/nova.hedwigia/64/1997/491.

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25

Privitera, Santo. "Poleis Massalia : da Artemidoro di Efeso a Eustazio di Tessalonica". Mélanges de l’École française de Rome. Antiquité 119, n.º 1 (2007): 41–49. http://dx.doi.org/10.3406/mefr.2007.10324.

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26

Yuan, Chao, Gang Li, Chang-sheng Wu, Hai-ying Wang, Zun-tian Zhao y Hong-xiang Lou. "A New Fatty Acid from the Endolichenic Fungus Massarina sp." Chemistry of Natural Compounds 51, n.º 3 (mayo de 2015): 415–17. http://dx.doi.org/10.1007/s10600-015-1305-9.

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27

Kohlmeyer, Jan y Brigitte Volkmann-Kohlmeyer. "Marine fungi from Aldabra, the Galapagos, and other tropical islands". Canadian Journal of Botany 65, n.º 3 (1 de marzo de 1987): 571–82. http://dx.doi.org/10.1139/b87-073.

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Saprobic higher marine fungi were collected in Aldabra (8 species, 1 variety), Galapagos (10 species), Hawaii (17 species), and Tobago (22 species). The material includes 26 ascomycetes, 1 basidiomycete, and 5 deuteromycetes. Lindra hawaiiensis (Maui) and Massarina thalassiae (Aldabra, Belize, Florida, Galapagos, India (Madras), Mexico (Baja California)) are new species. Distribution maps for the following species are presented: Cytospora rhizophorae, Didymosphaeria rhizophorae, Halosphaeria cucullata – Periconia prolifica, Lindra thalassiae, and Massarina thalassiae. Endemic marine fungi with saprobic modes of life have not been encountered.

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28

Grafton, Anthony. "Renaissance Readers and Ancient Texts: Comments on Some Commentaries". Renaissance Quarterly 38, n.º 4 (1985): 615–49. http://dx.doi.org/10.2307/2861952.

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In autumn, 1465, two humanists agreed to have a battle. Lorenzo Guidetti was a disciple and friend of Cristoforo Landino, the most prominent teacher of rhetoric in the Florentine Studio. Buonaccorso Massari was a pupil of a less famous master, Giovanni Pietro of Lucca. Naturally, Massari began the fight, with all the pretended friendliness and genuine desire to insert knives in backs that characterize the intellectual at his deadliest. Massari had heard that Landino would lecture on Cicero's Epistolae ad Familiares during the academic year 1465-6. “I am an eager student,” he wrote to Guidetti on 14 September, “and I would be grateful to know what Landino said when he explained the first letter. For in my view it is quite hard because of the historical questions involved, and few have the ability to tackle so great an enterprise.“

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29

Read, S. J., E. B. G. Jones y S. T. Moss. "Ultrastructural observations of asci, ascospores and appendages of Massarina armatispora (Ascomycota)". Mycoscience 38, n.º 2 (julio de 1997): 141–46. http://dx.doi.org/10.1007/bf02460849.

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30

Yusoff, M., S. T. Moss y E. B. G. Jones. "Ascospore ultrastructure of Pleospora gaudefroyi (Pleosporaceae, Loculoascomycetes, Ascomycotina)". Canadian Journal of Botany 72, n.º 1 (1 de enero de 1994): 1–6. http://dx.doi.org/10.1139/b94-001.

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Ascospore and spore appendage ultrastructure in Pleospora gaudefroyi is described. Ascospore walls comprise a three-layered mesosporium within which electron-dense bodies (melanin?) are deposited in two layers (W2, W3), a tripartite episporium (W4), and an outer mucilaginous sheath (exosporium?). The electron-dense inclusions in the mesosporium are compared with those in the conidia of Alternaria brassicicola and ascospores of Carbosphaerella leptosphaerioides. In the unitunicate marine Ascomycetes, ascospores with a sheath are uncommon, whereas in the Loculoascomycetes many species have such ascospores. The sheath in P. gaudefroyi is compared with those of Paraliomyces lentiferus, Massarina thalassiae, and Massarina armatispora (all Loculoascomycetes). Key words: appendage ontogeny, marine Ascomycotina, taxonomy, ultrastructure.

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31

Ryckegem, Gunther van y André Aptroot. "A new Massarina and a new Wettsteinina (Ascomycota) from freshwater and tidal reeds". Nova Hedwigia 73, n.º 1-2 (31 de agosto de 2001): 161–66. http://dx.doi.org/10.1127/nova.hedwigia/73/2001/161.

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32

Van Ryckegem, Gunther y André Aptroot. "A new Massarina and a new Wettsteinina (Ascomycota) from freshwater and tidal reeds". Nova Hedwigia 71, n.º 1-2 (7 de septiembre de 2000): 161–66. http://dx.doi.org/10.1127/nova.hedwigia/71/2001/161.

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33

Hyde, Kevin D. "Massarina velatospora and a New Mangrove-Inhabiting Species, M. ramunculicola sp. nov." Mycologia 83, n.º 6 (noviembre de 1991): 839. http://dx.doi.org/10.2307/3760447.

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34

Liew, Edward C. Y., Andre Aptroot y Kevin D. Hyde. "An Evaluation of the Monophyly of Massarina Based on Ribosomal DNA Sequences". Mycologia 94, n.º 5 (septiembre de 2002): 803. http://dx.doi.org/10.2307/3761695.

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35

Hyde, Kevin D. "Massarina Velatospora and a New Mangrove-Inhabiting Species, M. Ramunculicola Sp. Nov." Mycologia 83, n.º 6 (noviembre de 1991): 839–45. http://dx.doi.org/10.1080/00275514.1991.12026094.

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36

Tsui, K. M., K. D. Hyde y I. J. Hodgkiss. "Massarina proprietunicata sp. nov., from submerged wood in streams in Hong Kong". Mycological Research 103, n.º 12 (diciembre de 1999): 1575–78. http://dx.doi.org/10.1017/s095375629900876x.

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37

Feld, Steven, Ingrid Pfrang-Lewis y Nicolas Lewis. "Massana: Moments in Yaka Play and Ritual". Yearbook for Traditional Music 31 (1999): 219. http://dx.doi.org/10.2307/768060.

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38

Cesaretti, Enrico. "Nutrition as Dissolution: Paola Masino's Nascita e morte della massaia". Quaderni d'italianistica 28, n.º 2 (1 de junio de 2007): 143–62. http://dx.doi.org/10.33137/q.i..v28i2.8525.

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Many scholars agree that Paola Masino's novel Nascita e morte della massaia is to be read as an allegory protesting Fascism censorship and suppression of women's creative powers, a sort of literal grave-stone and defeat of the possibility of feminine imagination and creativity. By focusing on the implications of the alimentary discourse in the novel and on its "anorexic" protagonist, my essay wishes to complement the former reading. I suggest that an additional, perhaps less dramatic and tragic message may stem out of this depiction of a failure and a defeat. That is, a message that alludes to a fundamental faith in the resiliency of the powers of imagination and artistic creativity may actually, although marginally, be traced in the novel.

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39

Hyde, KD. "Tropical Australian freshwater fungi. I. Some ascomycetes". Australian Systematic Botany 5, n.º 1 (1992): 109. http://dx.doi.org/10.1071/sb9920109.

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Three fungi considered new to science were collected from submerged wood, at Millaa Millaa Falls in north Queensland, Australia. Ascagilis bipolaris, gen. et sp. nov., Aniptodera lignatilis, sp. nov. and Massarina australiensis, sp. nov. are described and illustrated.

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40

Végis, Graziella. "Le théâtre Massalia, la Friche la Belle de mai et la petite enfance". Spirale 35, n.º 3 (2005): 121. http://dx.doi.org/10.3917/spi.035.0121.

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41

Abdel-Wahab, Mohamed A., Ratnakar N. Asolkar, Patrik Inderbitzin y William Fenical. "Secondary metabolite chemistry of the marine-derived fungus Massarina sp., strain CNT-016". Phytochemistry 68, n.º 8 (abril de 2007): 1212–18. http://dx.doi.org/10.1016/j.phytochem.2007.01.020.

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42

Hyde, K. D. "Intertidal fungi from the mangrove fern Acrostichum speciosum, including Massarina acrostichi sp. nov." Mycological Research 93, n.º 4 (diciembre de 1989): 435–38. http://dx.doi.org/10.1016/s0953-7562(89)80037-1.

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43

Pham, Ha, Sergio Del Angel, Wei Xiong, Mark Astill, Holly Bethers, Tori Harris, Kristin Case, Sarah Sibio y Rong Mao. "eP386: Cystic fibrosis 165 pathogenic variants genotyping by MassARRAY". Genetics in Medicine 24, n.º 3 (marzo de 2022): S243—S244. http://dx.doi.org/10.1016/j.gim.2022.01.421.

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44

Zhou, Kun, Xin-Ge Zheng, Zhong-Yi Sun, Yu-Jing Gao, Zhong Wang, Wen-Bin Gao y Du-Qiang Luo. "A new compound from an endophytic fungus Bambusicola massarinia". Journal of Asian Natural Products Research 18, n.º 12 (29 de junio de 2016): 1200–1204. http://dx.doi.org/10.1080/10286020.2016.1194830.

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45

Owari, Satomi, Aiko Hayashi y Ken-ichiro Ishida. "Subcellular localization of minicircle DNA in the dinoflagellateAmphidinium massartii". Phycological Research 62, n.º 1 (4 de noviembre de 2013): 1–8. http://dx.doi.org/10.1111/pre.12037.

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46

Jurinke, Christian, Dirk Van Den Boom, Charles R. Cantor y Hubert Koster. "ChemInform Abstract: Automated Genotyping Using the DNA MassArray Technology." ChemInform 33, n.º 10 (22 de mayo de 2010): no. http://dx.doi.org/10.1002/chin.200210275.

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47

Halling, Kirsten. "Les oiseaux noirs de Massada by Olivia Elkaim". French Review 85, n.º 6 (2012): 1198–99. http://dx.doi.org/10.1353/tfr.2012.0195.

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48

Lee, Eun Sun, Jinik Hwang, Jun-Ho Hyung y Jaeyeon Park. "Detection of the Benthic Dinoflagellates, Ostreopsis cf. ovata and Amphidinium massartii (Dinophyceae), Using Loop-Mediated Isothermal Amplification". Journal of Marine Science and Engineering 9, n.º 8 (17 de agosto de 2021): 885. http://dx.doi.org/10.3390/jmse9080885.

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For the in situ and sensitive detection of benthic dinoflagellates, we have established an integrated loop-mediated isothermal amplification (LAMP) assay based on Ostreopsis cf. ovata and Amphidinium massartii. To detect the two species, a set of species-specific primers was constructed between the ITS gene and D1–D6 LSU gene, and the reaction temperature, time, and buffer composition were optimized to establish this method. In addition, the specificity of the LAMP primers was verified both in strains established in the laboratory and in field samples collected from the Jeju coastal waters, Korea. With the LAMP assay, the analysing time was within 45 to 60 min, which may be shorter than that with the conventional PCR. The detection sensitivity of the LAMP assay for O. cf. ovata or A. massartii was comparable to other molecular assays (PCR and quantitative PCR (qPCR)) and microscopy examination. The detection limit of LAMP was 0.1 cell of O. cf. ovata and 1 cell of A. massartii. The optimized LAMP assay was successfully applied to detect O. cf. ovata and A. massartii in field samples. Thus, this study provides an effective method for detecting target benthic dinoflagellate species, and could be further implemented to monitor phytoplankton in field surveys as an altenative.

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49

Shatskii, A. A., Igor D. Novikov y Nikolai S. Kardashev. "The Kepler problem and collisions of negative masses". Uspekhi Fizicheskih Nauk 181, n.º 4 (2011): 399. http://dx.doi.org/10.3367/ufnr.0181.201104e.0399.

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50

Corsi, Jacopo y Giorgio Aulisio. "The lions of Artemis. A die link study of the heavy drachms of Massalia". Revue numismatique 6, n.º 175 (2018): 147–92. http://dx.doi.org/10.3406/numi.2018.3417.

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