Acceder

Bibliografías temáticas / Mass spectrometry / Artículos de revistas

Artículos de revistas sobre el tema "Mass spectrometry"

Siga este enlace para ver otros tipos de publicaciones sobre el tema: Mass spectrometry.

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 20 de julio de 2024

Crea una cita precisa en los estilos APA, MLA, Chicago, Harvard y otros

Elija tipo de fuente:

Consulte los 50 mejores artículos de revistas para su investigación sobre el tema "Mass spectrometry".

Junto a cada fuente en la lista de referencias hay un botón "Agregar a la bibliografía". Pulsa este botón, y generaremos automáticamente la referencia bibliográfica para la obra elegida en el estilo de cita que necesites: APA, MLA, Harvard, Vancouver, Chicago, etc.

También puede descargar el texto completo de la publicación académica en formato pdf y leer en línea su resumen siempre que esté disponible en los metadatos.

Explore artículos de revistas sobre una amplia variedad de disciplinas y organice su bibliografía correctamente.

1

KASAMA, Takeshi. "Biological Mass Spectrometry. Quadrupole Mass Spectrometer." Journal of the Mass Spectrometry Society of Japan 44, n.º 3 (1996): 393–405. http://dx.doi.org/10.5702/massspec.44.393.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

2

Glish, Gary L. y David J. Burinsky. "Hybrid mass spectrometers for tandem mass spectrometry". Journal of the American Society for Mass Spectrometry 19, n.º 2 (febrero de 2008): 161–72. http://dx.doi.org/10.1016/j.jasms.2007.11.013.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

3

Busch, Kenneth L., Gary L. Glish, Scott A. McLuckey y John J. Monaghan. "Mass spectrometry/mass spectrometry: techniques and applications of tandem mass spectrometry". Analytica Chimica Acta 237 (1990): 509. http://dx.doi.org/10.1016/s0003-2670(00)83956-2.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

4

Futrell, Jean H. "Mass spectrometry/mass spectrometry: Techniques and applications of tandem mass spectrometry". Microchemical Journal 41, n.º 2 (abril de 1990): 246–47. http://dx.doi.org/10.1016/0026-265x(90)90124-n.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

5

Moriarty, F. "Mass spectrometry/mass spectrometry. Techniques and applications of tandem mass spectrometry". Environmental Pollution 61, n.º 3 (1989): 261. http://dx.doi.org/10.1016/0269-7491(89)90246-7.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

6

Cooks, R. G. "Mass Spectrometry/Mass Spectrometry. Techniques and Applications of Tandem Mass Spectrometry". International Journal of Mass Spectrometry and Ion Processes 93, n.º 2 (octubre de 1989): 265–66. http://dx.doi.org/10.1016/0168-1176(89)80103-x.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

7

Pinkston, J. David, Martin Rabb, J. Throck Watson y John Allison. "New time‐of‐flight mass spectrometer for improved mass resolution, versatility, and mass spectrometry/mass spectrometry studies". Review of Scientific Instruments 57, n.º 4 (abril de 1986): 583–92. http://dx.doi.org/10.1063/1.1138874.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

8

Glish, Gary L. y Scott A. McLuckey. "Hybrid Instruments for Mass Spectrometry/Mass Spectrometry". Instrumentation Science & Technology 15, n.º 1 (enero de 1986): 1–36. http://dx.doi.org/10.1080/10739148608543593.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

9

Charles, M. Judith y Yves Tondeur. "Choosing between high-resolution mass spectrometry and mass spectrometry/mass spectrometry environmental applications". Environmental Science & Technology 24, n.º 12 (diciembre de 1990): 1856–60. http://dx.doi.org/10.1021/es00082a011.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

10

KONDO, Fumio y Ken-ichi HARADA. "Biological Mass Spectrometry. Mass Spectrometric Analysis of Cyanobacterial Toxins." Journal of the Mass Spectrometry Society of Japan 44, n.º 3 (1996): 355–76. http://dx.doi.org/10.5702/massspec.44.355.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

11

Wu, Junhan, Wenpeng Zhang y Zheng Ouyang. "On-Demand Mass Spectrometry Analysis by Miniature Mass Spectrometer". Analytical Chemistry 93, n.º 15 (5 de abril de 2021): 6003–7. http://dx.doi.org/10.1021/acs.analchem.1c00575.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

12

NOHMI, Takashi y Tetsuya MIYAGISHI. "Future Mass from Miniaturized Mass Spectrometry to Micro Mass Spectrometry." Journal of the Mass Spectrometry Society of Japan 51, n.º 1 (2003): 54–66. http://dx.doi.org/10.5702/massspec.51.54.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

13

Guerrera, Ida Chiara y Oliver Kleiner. "Application of Mass Spectrometry in Proteomics". Bioscience Reports 25, n.º 1-2 (4 de febrero de 2005): 71–93. http://dx.doi.org/10.1007/s10540-005-2849-x.

Texto completo

Resumen

Mass spectrometry has arguably become the core technology in proteomics. The application of mass spectrometry based techniques for the qualitative and quantitative analysis of global proteome samples derived from complex mixtures has had a big impact in the understanding of cellular function. Here, we give a brief introduction to principles of mass spectrometry and instrumentation currently used in proteomics experiments. In addition, recent developments in the application of mass spectrometry in proteomics are summarised. Strategies allowing high-throughput identification of proteins from highly complex mixtures include accurate mass measurement of peptides derived from total proteome digests and multidimensional peptide separations coupled with mass spectrometry. Mass spectrometric analysis of intact proteins permits the characterisation of protein isoforms. Recent developments in stable isotope labelling techniques and chemical tagging allow the mass spectrometry based differential display and quantitation of proteins, and newly established affinity procedures enable the targeted characterisation of post-translationally modified proteins. Finally, advances in mass spectrometric imaging allow the gathering of specific information on the local molecular composition, relative abundance and spatial distribution of peptides and proteins in thin tissue sections.

Los estilos APA, Harvard, Vancouver, ISO, etc.

14

ITO, Yuji y Masahiro MATSUI. "Mass Spectrometry". Journal of the Japan Society of Colour Material 63, n.º 7 (1990): 419–29. http://dx.doi.org/10.4011/shikizai1937.63.419.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

15

Lederman, Lynne. "Mass Spectrometry". BioTechniques 46, n.º 6 (mayo de 2009): 399–401. http://dx.doi.org/10.2144/000113165.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

16

Yates, John R. "Mass spectrometry". Trends in Genetics 16, n.º 1 (enero de 2000): 5–8. http://dx.doi.org/10.1016/s0168-9525(99)01879-x.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

17

Burlingame, A. L., D. S. Millington, D. L. Norwood y D. H. Russell. "Mass spectrometry". Analytical Chemistry 62, n.º 12 (15 de junio de 1990): 268–303. http://dx.doi.org/10.1021/ac00211a020.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

18

Burlingame, A. L., D. Maltby, D. H. Russell y P. T. Holland. "Mass spectrometry". Analytical Chemistry 60, n.º 12 (15 de junio de 1988): 294–342. http://dx.doi.org/10.1021/ac00163a021.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

19

Burlingame, A. L., Robert K. Boyd y Simon J. Gaskell. "Mass Spectrometry". Analytical Chemistry 68, n.º 12 (enero de 1996): 599–652. http://dx.doi.org/10.1021/a1960021u.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

20

Burlingame, A. L., Robert K. Boyd y Simon J. Gaskell. "Mass Spectrometry". Analytical Chemistry 70, n.º 16 (agosto de 1998): 647–716. http://dx.doi.org/10.1021/a1980023+.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

21

Burlingame, A. L., T. A. Baillie y D. H. Russell. "Mass spectrometry". Analytical Chemistry 64, n.º 12 (15 de junio de 1992): 467–502. http://dx.doi.org/10.1021/ac00036a025.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

22

Kinter, Michael. "Mass spectrometry". Analytical Chemistry 67, n.º 12 (15 de junio de 1995): 493–97. http://dx.doi.org/10.1021/ac00108a034.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

23

Caprioli, Richard y Alan Wu. "Mass Spectrometry". Analytical Chemistry 65, n.º 12 (15 de junio de 1993): 470–74. http://dx.doi.org/10.1021/ac00060a619.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

24

Burlingame, A. L., Robert K. Boyd y Simon J. Gaskell. "Mass Spectrometry". Analytical Chemistry 66, n.º 12 (junio de 1994): 634–83. http://dx.doi.org/10.1021/ac00084a024.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

25

Burlingame, A. L., Thomas A. Baillie y Peter J. Derrick. "Mass spectrometry". Analytical Chemistry 58, n.º 5 (abril de 1986): 165–211. http://dx.doi.org/10.1021/ac00296a015.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

26

Grotemeyer, Jürgen, Klaus G. Heumann y Wolf D. Lehmann. "Mass spectrometry". Analytical and Bioanalytical Chemistry 386, n.º 1 (8 de agosto de 2006): 21–23. http://dx.doi.org/10.1007/s00216-006-0653-5.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

27

Van Thuijl, J. "Mass spectrometry". TrAC Trends in Analytical Chemistry 5, n.º 3 (marzo de 1986): IX—X. http://dx.doi.org/10.1016/0165-9936(86)85017-8.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

28

Grotemeyer, J. "Mass spectrometry". Analytical and Bioanalytical Chemistry 377, n.º 7-8 (1 de diciembre de 2003): 1097. http://dx.doi.org/10.1007/s00216-003-2292-4.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

29

Vickerman, J. C. "Mass spectrometry". Endeavour 11, n.º 2 (enero de 1987): 108. http://dx.doi.org/10.1016/0160-9327(87)90265-1.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

30

Jannetto, Paul J. y Darlington Danso. "Mass spectrometry". Clinical Biochemistry 82 (agosto de 2020): 1. http://dx.doi.org/10.1016/j.clinbiochem.2020.06.003.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

31

P, D. "Mass Spectrometry". Journal of Molecular Structure 160, n.º 1-2 (agosto de 1987): 183. http://dx.doi.org/10.1016/0022-2860(87)87016-3.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

32

Robinson, Carol y Robert J. Cotter. "Mass spectrometry". Proteins: Structure, Function, and Genetics 33, S2 (1998): 1–2. http://dx.doi.org/10.1002/(sici)1097-0134(1998)33:2+<1::aid-prot1>3.0.co;2-m.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

33

Lhotka, Radek y Petr Vodička. "Aerosol Mass Spectrometry". Chemické listy 118, n.º 5 (15 de mayo de 2024): 254–62. http://dx.doi.org/10.54779/chl20240254.

Texto completo

Resumen

Mass spectrometry is widely used in various scientific fields. In atmospheric chemistry, there has been a long call for a detailed on-line analysis of the chemical composition of aerosol particles (i.e., particles in the solid or liquid state) in the atmosphere resulting in the development of the so-called aerosol mass spectrometers in the past 20 years. These instruments allow the measurement of the chemical composition of particles with sizes of ca. 50–800 nm, typically at minute resolution. Their development and possible applications are discussed in this review.

Los estilos APA, Harvard, Vancouver, ISO, etc.

34

Termopoli, Veronica, Maurizio Piergiovanni, Davide Ballabio, Viviana Consonni, Emmanuel Cruz Muñoz y Fabio Gosetti. "Condensed Phase Membrane Introduction Mass Spectrometry: A Direct Alternative to Fully Exploit the Mass Spectrometry Potential in Environmental Sample Analysis". Separations 10, n.º 2 (17 de febrero de 2023): 139. http://dx.doi.org/10.3390/separations10020139.

Texto completo

Resumen

Membrane introduction mass spectrometry (MIMS) is a direct mass spectrometry technique used to monitor online chemical systems or quickly quantify trace levels of different groups of compounds in complex matrices without extensive sample preparation steps and chromatographic separation. MIMS utilizes a thin, semi-permeable, and selective membrane that directly connects the sample and the mass spectrometer. The analytes in the sample are pre-concentrated by the membrane depending on their physicochemical properties and directly transferred, using different acceptor phases (gas, liquid or vacuum) to the mass spectrometer. Condensed phase (CP) MIMS use a liquid as a medium, extending the range to new applications to less-volatile compounds that are challenging or unsuitable to gas-phase MIMS. It directly allows the rapid quantification of selected compounds in complex matrices, the online monitoring of chemical reactions (in real-time), as well as in situ measurements. CP-MIMS has expanded beyond the measurement of several organic compounds because of the use of different types of liquid acceptor phases, geometries, dimensions, and mass spectrometers. This review surveys advancements of CP-MIMS and its applications to several molecules and matrices over the past 15 years.

Los estilos APA, Harvard, Vancouver, ISO, etc.

35

NAGAO, Keisuke. "Fundamentals of Mass Spectrometry -Isotope Ratio Mass Spectrometry-". Journal of the Mass Spectrometry Society of Japan 59, n.º 2 (2011): 35–49. http://dx.doi.org/10.5702/massspec.59.35.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

36

Musselman, Brian D. "K. Busch, G. Glish and S. Mcluckey. Mass spectrometry/mass spectrometry: Techniques and applications of tandem mass spectrometry, VCH publishing, New York, 1988. Mass Spectrometry/Mass Spectrometry". Biological Mass Spectrometry 18, n.º 10 (octubre de 1989): 942. http://dx.doi.org/10.1002/bms.1200181017.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

37

LaiHing, K., P. Y. Cheng, T. G. Taylor, K. F. Willey, M. Peschke y M. A. Duncan. "Photodissociation in a reflectron time-of-flight mass spectrometer: a novel mass spectrometry/mass spectrometry configuration for high-mass systems". Analytical Chemistry 61, n.º 13 (julio de 1989): 1458–60. http://dx.doi.org/10.1021/ac00188a031.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

38

Meier, Heiko y Gottfried Blaschke. "Capillary electrophoresis–mass spectrometry, liquid chromatography–mass spectrometry and nanoelectrospray-mass spectrometry of praziquantel metabolites". Journal of Chromatography B: Biomedical Sciences and Applications 748, n.º 1 (octubre de 2000): 221–31. http://dx.doi.org/10.1016/s0378-4347(00)00397-2.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

39

Dogra, Akshay. "A Thorough Examination of the Recent Advances in Mass Spectrometry". International Journal for Research in Applied Science and Engineering Technology 11, n.º 7 (31 de julio de 2023): 1731–41. http://dx.doi.org/10.22214/ijraset.2023.54964.

Texto completo

Resumen

Abstract: Mass spectrometry has become an essential tool in pharmaceutical analysis, revolutionizing drug development, quality assurance, and our understanding of complex biological systems. This review provides a comprehensive overview of recent advances in mass spectrometry for pharmaceutical analysis. We discuss the fundamentals of mass spectrometry, including ionization and mass analysis principles, as well as the various types of mass spectrometers used in pharmaceutical analysis. We explore high-resolution mass spectrometry (HRMS), tandem mass spectrometry (MS/MS), ambient ionization mass spectrometry, and mass spectrometry imaging (MSI), highlighting their applications in drug characterization, quantification, imaging, and biomarker discovery. Furthermore, we examine the challenges faced by mass spectrometry, such as matrix effects and data interpretation, and discuss emerging trends and future perspectives. By understanding the recent advancements and addressing the challenges, mass spectrometry can continue to drive advancements in pharmaceutical analysis and quality assurance

Los estilos APA, Harvard, Vancouver, ISO, etc.

40

Cooks, R. G., K. A. Cox y J. D. Williams. "High-performance mass spectrometry with the ion trap mass spectrometer". Journal of Protein Chemistry 11, n.º 4 (agosto de 1992): 376–77. http://dx.doi.org/10.1007/bf01673733.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

41

Budzikiewicz, H. "Selected reviews on mass spectrometric topics. XXVIII. Tandem mass spectrometry". Mass Spectrometry Reviews 8, n.º 2 (marzo de 1989): 119. http://dx.doi.org/10.1002/mas.1280080204.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

42

Budzikiewicz, H. "Selected reviews on mass spectrometric topics. XLV. Pyrolysis-mass spectrometry". Mass Spectrometry Reviews 11, n.º 3 (mayo de 1992): 247. http://dx.doi.org/10.1002/mas.1280110304.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

43

Budzikiewicz, H. "Selected reviews on mass spectrometric topics. XLVII. Accelerator mass spectrometry". Mass Spectrometry Reviews 11, n.º 5 (septiembre de 1992): 445. http://dx.doi.org/10.1002/mas.1280110505.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

44

Roberts, Norman B., Brian N. Green y Michael Morris. "Potential of electrospray mass spectrometry for quantifying glycohemoglobin". Clinical Chemistry 43, n.º 5 (1 de mayo de 1997): 771–78. http://dx.doi.org/10.1093/clinchem/43.5.771.

Texto completo

Resumen

Abstract An electrospray ionization–mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3–15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced directly into a mass spectrometer. The resulting mass spectra were then processed to estimate the percentage of glycohemoglobin present in the sample. Total analysis time, including plotting the spectra and computing the percentage of glycation, was ∼3 min. The imprecision (CV) of the method was <5.1% for inter- and intrabatch analyses for total glycohemoglobin in the range 3.6–14%. Comparison of the mass spectrometric results with those from established affinity chromatographic procedures showed good overall agreement. The relative glycation of the α- and β-chains was determined directly and was shown to be constant (0.64:1) over the glycation range measured. Only single glucose attachment to both the α- and β-chains was observed.

Los estilos APA, Harvard, Vancouver, ISO, etc.

45

Tian, Qingguo y Steven J. Schwartz. "Mass Spectrometry and Tandem Mass Spectrometry of Citrus Limonoids". Analytical Chemistry 75, n.º 20 (octubre de 2003): 5451–60. http://dx.doi.org/10.1021/ac030115w.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

46

Shoji, Yuki, Mari Yotsu-Yamashita, Teruo Miyazawa y Takeshi Yasumoto. "Electrospray Ionization Mass Spectrometry of Tetrodotoxin and Its Analogs: Liquid Chromatography/Mass Spectrometry, Tandem Mass Spectrometry, and Liquid Chromatography/Tandem Mass Spectrometry". Analytical Biochemistry 290, n.º 1 (marzo de 2001): 10–17. http://dx.doi.org/10.1006/abio.2000.4953.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

47

Cooks, R. Graham, Alan K. Jarmusch, Christina R. Ferreira y Valentina Pirro. "Skin molecule maps using mass spectrometry". Proceedings of the National Academy of Sciences 112, n.º 17 (20 de abril de 2015): 5261–62. http://dx.doi.org/10.1073/pnas.1505313112.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

48

Van Berkel, Gary J., Gary L. Glish, Scott A. McLuckey y Albert A. Tuinman. "High-pressure ammonia chemical ionization mass spectrometry and mass spectrometry/mass spectrometry for porphyrin structure determination". Energy & Fuels 4, n.º 6 (noviembre de 1990): 720–29. http://dx.doi.org/10.1021/ef00024a018.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

49

Hasegawa, Yuki, Misako Iga, Masahiko Kimura, Yosuke Shigematsu y Seiji Yamaguchi. "Prenatal diagnosis for organic acid disorders using two mass spectrometric methods, gas chromatography mass spectrometry and tandem mass spectrometry". Journal of Chromatography B 823, n.º 1 (agosto de 2005): 13–17. http://dx.doi.org/10.1016/j.jchromb.2005.04.020.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

50

Konstantinov, M. A., D. D. Zhdanov y I. Yu Toropygin. "Quantitative mass spectrometry with <sup>18</sup>O labelling as an alternative approach for determining protease activity: an example of trypsin". Biological Products. Prevention, Diagnosis, Treatment 24, n.º 1 (6 de febrero de 2024): 46–60. http://dx.doi.org/10.30895/2221-996x-2024-24-1-46-60.

Texto completo

Resumen

SCIENTIFIC RELEVANCE. In the quality control of proteolytic enzyme components of medicinal products, the activity of proteases is determined by spectrophotometry, which involves measuring the amidase or esterase activity using a synthetic substrate and the proteolytic activity using the Anson method. These methods require special substrates and have low sensitivity; their specificity may be insufficient, which may lead to serious errors. Quantitative mass spectrometry is an alternative approach to protease activity assays, which involves adding an isotope-labelled peptide to hydrolysates of the test enzyme. This approach allows determining the activity of proteases, notably, by the hydrolysis of specific peptide bonds, while simultaneously confirming the identity and specificity of the test sample. Quantitative mass spectrometry has high sensitivity and does not require special substrates.AIM. This study aimed to investigate the possibility of enzymatic activity assay and enzyme identification by quantitative mass spectrometry with 18O labelling through an example of trypsin with casein.MATERIALS AND METHODS. The study used trypsin, casein, and H218O (Izotop, Russia). Peptide separation was performed using an Agilent 1100 HPLC system; mass spectra were obtained using a Bruker Ultraflex II MALDI-TOF/TOF mass spectrometer. Quantitative mass spectrometry was performed using a standard peptide, which was obtained from casein by tryptic digestion and HPLC purification. For 18O labelling, the authors dried the peptide and incubated it in H218О water. The quantitative analysis of the product was carried out using MALDI-TOF mass spectrometry. The authors used quantitative mass spectrometry with 18O labelling to determine enzymatic activity and calculate the Michaelis constant (KM).RESULTS. Following the tryptic digestion of casein, the authors identified the fragments corresponding to casein chains. The authors produced the isotope-labelled standard peptide and calculated its concentration using mass spectrometry. The authors determined the rate of casein digestion by trypsin and calculated the KM for trypsin, which was 13.65±0.60 μM. The standard deviation for repeated measurements showed that the mass-spectrometric method had a lower error of measurement than the spectrophotometric method. The sensitivity threshold for the mass-spectrometric method was 0.50±0.08 μM.CONCLUSIONS. The results obtained with trypsin confirm the possibility of enzymatic activity determination by the proposed method of quantitative mass spectrometry with 18O labelling. According to the sensitivity evaluation results, this method can be used for the simultaneous determination of enzyme activity, identity, and specificity. The proposed mass spectrometry approach is universal, it does not require expensive materials and reagents, and it can be easily adapted to determine the activity of virtually any protease.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Ofrecemos descuentos en todos los planes premium para autores cuyas obras están incluidas en selecciones literarias temáticas. ¡Contáctenos para obtener un código promocional único!