Tesis sobre el tema "Legal status of stem cells"
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Swanepoel, Magdaleen. "Embryonic stem cell research and cloning a proposed legislative framework in context of legal status and personhood /". Diss., Pretoria : [s.n.], 2006. http://upetd.up.ac.za/thesis/available/etd-07312007-150150/.
Perez, Castiglioni Monica Patricia. "Le statut juridique des cellules souches : de la greffe d’organes à la thérapie cellulaire". Electronic Thesis or Diss., Paris 8, 2021. http://www.theses.fr/2021PA080048.
Stem cells as cellular products for therapeutic purposes (PCT) or as advanced therapy drugs (ITNs) within the framework of regenerative medicine have revolutionized the medicine of the 21st century. Faced with recent discoveries of new stem cells created by researchers (parthenotes, cloned stem cells, iPS cells), other possibilities for regenerative therapy are emerging over time.The law, which has always accompanied the scientific and technical development of cell therapy since the 17th century, must be more present than ever to protect human beings who lend themselves to new treatments or to experimentation. The historical development of this therapeutic revolution allows us to show the importance of legal and ethical reflection for scientific progress.Old questions, such as the status of the prenatal being and the authorization for cryopreservation of autologous tissues or cells, are re-emerging in the face of the presence of supernumerary human embryonic stem cells and the success of regenerative therapy. Teratogenic treatments and episodes of child abuse during pregnancy have destroyed or damaged thousands of unborn children. Recognition of prenatal life is offered in certain circumstances to protect the embryo and fetus before birth
Rugg-Gunn, Peter. "Epigenetic status of human embryonic stem cells". Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614294.
Gough, Fionnuala Mary. "Irish ostriches, embryos and stem cells". Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/irish-ostriches-embryos-and-stem-cells(3a3aef52-1ab5-43d6-9bf8-b29ce28b5e32).html.
Nortjé, Nico. "The moral status of embryonic stem cell research in the South African context /". Link to the online version, 2007. http://hdl.handle.net/10019.1/1372.
Batsivari, Antoniana. "Studying the cell cycle status during haematopoietic stem cell development". Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/25802.
Nortje, Nico. "The moral status of embryonic stem cell research in the South African context". Thesis, Stellenbosch : University of Stellenbosch, 2007. http://hdl.handle.net/10019.1/1372.
Should surplus embryos which are destined to be discarded be protected at all cost, to the extent that they cannot contribute to medical knowledge - knowledge which could benefit society at large? Are embryos people or merely items of property? Different moral theories address these questions in different ways. Deontologists argue that the end never justifies the means and that the right not to be killed is more fundamental than the obligation to save. Utilitarians, on the other hand, argue that certain criteria should be met before moral significance can be contributed to an entity. The question of the moral status of the embryo is, as my discussion will show, one of the most widely discussed issues in the history of bioethics. Extensive literature exists on the topic. This study holds that an Ethics of Responsibility (ER) should by applied when answering the questions posed above as it encourages one to accept responsibility for the choices or decisions made and to defend them accordingly. I have endeavoured to answer the question of the personhood and rights of the embryo within the framework of the Ethics of Responsibility. Although these concepts overlap in many ways they remain central to the debate surrounding the sanctioning or prevention of the use of human embryonic stem cells in research. After identifying the micro-issues surrounding the human embryonic stem cell debate and explaining why both the deontologist and utilitarians fail to provide any adequate answers in this respect, I turn my attention to macro-issues such as safety concerns surrounding the usages and storage of stem cells. Commercialization, power issues, accessibility and the allocation of limited resources are also examined. Living in a society such as South Africa one cannot be blind to the inequalities of our health system. On a macro level I cannot but conclude that stem cell research does not seem to be a viable exercise within the South African context. South Africa faces a health care crisis far greater than the benefits stem cell research currently has to offer. However, the need still exists for a policy to guide future lawmakers who might need to address stem cell research and to guide decisions and actions. This brings me to my final chapter, namely proposing a morally justified policy for South Africa. I propose a policy which respects and values the autonomy of the progenitors’ choices (provided they have not been coerced) and which focuses on the beneficence of the greater society. Furthermore, it is paramount that the goal of any stem cell research should be for therapeutic use ONLY. Before commencing with the extraction of the stem cells, scientists should be obligated first to present convincing evidence that they have tried alternative ways to reach the same result. Once this has been proven, a regulatory body could issue the scientist/team with a license to undertake the specific research with a specific therapy as goal in order to prevent abuse. If they are found guilty of any unethical conduct their licenses should be revoked and an investigation launched.
Marzec-Schmidt, Katarzyna. "Deep convolutional neural networks accurately predict the differentiation status of human induced pluripotent stem cells". Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-19420.
Harmon, Shawn H. E. "Health research, (bio)technology, regulation & values : operationalising socio-moral values in the legal setting". Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/9794.
Alsheikh, Manal. "Impact of the Maturation Status of Osteoblasts on Their Hematopoietic Regulatory Activity". Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/35899.
Qian, Xu [Verfasser]. "ALDH1-positive cancer stem-like cells enrich in nodal metastases of oropharyngeal squamous cell carcinoma independently of HPV-status / Xu Qian". Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2013. http://d-nb.info/1043197427/34.
McCarrey, Sariah Cottrell. "Personhood and Cloning: Modern Applications and Ethics of Stem Cell and Cloning Technology". BYU ScholarsArchive, 2013. https://scholarsarchive.byu.edu/etd/4170.
Reis, Silas Mendes dos. "A certeza da objetividade cobrindo a incerteza da subjetividade: um estudo de caso - a ADIn 3.510/DF e a presença de elementos jurídicos e extrajurídicos na argumentação constitucional". Pontifícia Universidade Católica de São Paulo, 2010. https://tede2.pucsp.br/handle/handle/8994.
By establishing a foundation for final decisions of merit, judicial authority uses several elements, such as language, interpretation and discourse, which will comprise the content of the sentence or judgment, culminating in the grounds or lack of grounds for the request. However, in collegiate judgments, even if the conclusion of decisions pronounced by judges is the same, the votes rendered take different paths, characterized by the oneness of foundations chosen, even in cases of decisions rendered unanimously. Although there is no uniformity of understanding, the conclusions presented are considered acceptable, with the interpretation made by winning votes prevailing. This dissertation intends to demonstrate the presence of extrajudicial elements in the formation of the judge's conviction, characterized and raised by subjectivity. The certainty of objectivity functions as a cloak spread over subjectivity. The demonstrative method and study of arguments will be used according to the structure elaborated by Chaïm Perelman and also from the perspective of legal and non-legal argumentation found in the judgment of ADIn 3510/DF on the use of embryonic stem cells obtained from human embryos produced by in vitro fertilization and not used in the respective procedure. The study will consist of showing there is a merger of objective and subjective elements in the foundation related to the belief of the legal exegete. It shall be concluded that subjectivity is tied to experience and culture found in the foundation for the votes, encompassing valued judgments and their arbitrary (discretionary) application, implicitly containing intuition. The scope of the dissertation will be a case study (ADIn 3.510/DF) and the validity of the procedure, starting with the choice of a sample of the vote population emanating from the Ministers of the Federal Supreme Court, and checking whether it constitutes an outline of the population of decisions handed down by jurisdictional entities
O julgador, ao fundamentar as decisões terminativas de mérito, utiliza-se de vários elementos tais como a linguagem, a interpretação e o discurso, os quais comporão o conteúdo da sentença ou acórdão, culminando na procedência ou improcedência do pedido. Todavia, nos julgamentos colegiados, ainda que a conclusão das decisões proferidas pelos julgadores seja a mesma, os votos exarados trilham caminhos diversos, caracterizando-se pela não unicidade dos fundamentos escolhidos, mesmo nos casos de decisões prolatadas por unanimidade. Embora ausente a uniformidade de entendimento, as conclusões apresentadas são consideradas aceitáveis, prevalecendo a interpretação feita pelos votos vencedores. Esta dissertação pretende demonstrar a presença de elementos extrajurídicos na formação da convicção do julgador, caracterizados e deflagrados pela sua subjetividade. A certeza da objetividade atua como um manto estendido sobre a subjetividade. Será utilizado o método demonstrativo e o estudo dos argumentos de acordo com a estrutura elaborada por Chaïm Perelman e também sob a ótica da argumentação jurídica e não-jurídica constantes do julgamento da ADIn 3.510/DF, sobre a utilização de células-tronco embrionárias obtidas de embriões humanos produzidos por fertilização in vitro e não utilizados no respectivo procedimento. A investigação consistirá em evidenciar que há fusão de elementos objetivos e subjetivos na fundamentação, relacionados à crença do exegeta jurídico. Concluir-se-á que a subjetividade está ligada à experiência e cultura, contidas na fundamentação dos votos, englobando juízos valorativos e sua aplicação arbitrária (discricionária), contendo implicitamente a intuição. A dissertação terá como escopo um estudo de caso (ADIn 3.510/DF) e a validade do procedimento, partindo-se da escolha de uma amostra da população de votos emanados pelos Ministros do Supremo Tribunal Federal, verificando-se se constitui um traço da população de decisões proferidas pelos órgãos jurisdicionais
Drouin, Érika Véronique. "L'utilisation des cellules souches embryonnaires à des fins thérapeutiques". Thèse, 2003. http://hdl.handle.net/1866/2381.
The embryonic stem cells discovery and the immense therapeutic potential glven to them has created big hopes in the world of today. The appearance of new revolutionary therapies to treat sorne of the most serious known diseases are now conceivable. However, the treatment of life to its earliest stage is questionned. The legal status recognized to the foetus and the embryo has, in fact, a direct effect to the research area and industry as weil as to its therapeutic use. Therefore, we have examined and studied the CUITent canadian law with respect to the legal status of the foetus and embryo. Following this study, we have noticed the uncertainty that prevails in Canada concerning the said legal status. Afierwards, we have examined ail the different canadian norms and regulations already established regarding the use of embryonic stem cells for therapeutic ends. We also did the comparaison between those norms and regulations so as to see their differences and similarities. It appears from our analysis that ail the canadian litterature generally treat the subject in the same way and that there have been few changes from 1993 up until now with respect to the forbidden researchs activities in Canada. We also have analysed the foreign law standards and regulations in United States and Great Britain concerning those forbidden researchs activities. We did the exercise of comparing the state of the law in these three countries with different parameters. It emerges from that that Great Britain is the most liberal country, United States being the most conservative and Canada being in between them.
"Mémoire présenté à la Faculté des études supérieures en vue de l'obtention du grade de Maîtrise en droit (L.L.M.) Option recherche"
Hsia, Kan-Tai y 夏堪臺. "A Legal Analysis of Utilizing Stem Cells". Thesis, 2006. http://ndltd.ncl.edu.tw/handle/90604756824187488935.
國立交通大學
科技法律研究所
94
A stem cell is a cell from the embryo, fetus, or adult that has a unique capacity to renew itself and to give rise to specialized cell types. It also can differentiate into any type of tissue or organ, presenting the enticing prospect that they could one day be used to replace diseased or damaged cells and tissue. In 1998 cultures of human pluripotent stem cells were first created in the laboratory of James Thomson at the University of Wisconsin-Madison (Science 282, 1145; 1998). Research using stem cells is an extremely active area of current biomedical inquiry. Research efforts have focused on spinal cord injury, multiple sclerosis, Parkinson’s disease, Alzheimer’s disease, diabetes, and other diseases or conditions. Scientists hope to use specialized cells to replace dysfunctional cells in the brain, spinal cord, pancreas, and other organs. Other potential applications for human stem cell cultures include uses for studying fundamental processes of human development or for toxicological testing and drug design. Non-human animal stem cell lines may also be used to produce genetically modified animals. However, the rapid march of stem cell research doesn’t give society much time to figure out a coherent response to its discoveries, both because of its extraordinary promise and because of relevant legal and ethical issues. Controversy surrounds the derivation of stem cells from human embryos and fetuses. In order to derive or extract the stem cells found within the embryo, the embryo is destroyed in the removal process. Given the moral implications of this extraction and the sanctity attached to embryos by many groups and individuals around the world, it is unsurprising that the question of how the research should proceed has spawned an energetic debate. This study employs the comparative method based on Laws and Regulations in Taiwan, foreign literature review and Anglo-American Law to discuss the likely disputes on using and regulating embryonic stem cells. Furthermore, there are many unresolved ethical issues related to the clinical and experimental use of adult stem cells such as umbilical cord blood. These issues include determination of ethical procedures for informed consent and institution review border for adult stem cell donation to public banks, to private banks, and for research and treatment. For example, legal and ethical issues related to privacy, confidentiality, and ownership of cord blood units are complex and controversial. There is also considerable debate regarding the ethics of commercial cord blood banking, particularly related to the availability of this potentially valuable resource for clinical use and research. Finally, the legal systems concerning patentability of stem cells are different in the U.S., Europe and Taiwan. This study discusses the advantages and disadvantages of grant of patent for stem cells, the feasibility and limitation to the protection for the patent of stem cells.
Chen, Chun-liang y 陳俊良. "The Legal Issues and Regulation of Umbilical Cord Blood Stem Cells". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/27700584493834915316.
國立高雄第一科技大學
科技法律研究所
97
Technology advancement and medical devices technology development have brought great contributions to human civilization. Stem Cell, extracted from the Umbilical Cord Blood, is one of the significant discoveries in our history without doubt. Cord Blood was once deemed medical waste and of no value at all, while it is nowadays medical treasure due to its stem cells. Therefore, his study will first clarify the ownership of the Cord Blood for further discussion about its related legal issues. In recent years, Cord Blood Registry (Cord Blood Banks) keep advising and promoting the benefits of preserving the Cord Blood. Many parents-to-be are wondering whether to preserve the Cord Blood for future utilization. If they determine to preserve the Cord Blood, they would next have to conduct a Contract with the Cord Blood Bank, public or private. What exactly is the nature of the Contract? How do the parties arrange the articles in the Contract? Since our Department of Health, Executive Yuan (DOH) has drafted the “Template of Standardized Contract for the Cord Blood Preservation”, this study will present several critical discussions to examine the articles of the DOH Template. This study will also provide concrete suggestions for parents-to-be to refer to when they conduct a Contract of Cord Blood Preservation in the future. This study would in addition introduce the regulation models of Cord Blood Banks in European Union and the United States, and review the current management regulation of Cord Blood Banks in Taiwan. This study will then provide for the Government’s reference regarding laws and regulations applicable to practical management of Cord Blood Banks.
Leu, Ji-Ying y 呂姬瑩. "Effects of Antioxidants on Senescence Status of Human Mesenchymal Stem Cells". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/87993784325939618869.
國立交通大學
生物科技學系
101
Mesenchymal stem cells (MSCs) are the multipotent stem cells that can give rise to mesenchyme-lineage cells, such as osteoblasts, adipocytes, chondrocytes and myoblasts. MSCs can be isolated from various tissues including bone marrow, adipose tissue, and umbilical cord blood etc. Recently, MSCs have been represented as a promising cell source for further cellular therapy. In spite of the strong self-renewal property, MSCs undergo cellular senescence during expanded cultivation in vitro. It is well-known that the oxidative stress plays an important role for cell aging. In this study, we focused on the relationship between cellular oxidative status and cultivated senescence in bone marrow-derived MSCs (BMMSCs) and cord blood-derived MSCs (CBMSCs). We examined the cellular oxidative stress by culturing cells with antioxidants, kinetin and epigallocatechin-3-gallate (EGCG). To understand the effects of antioxidants supplementation on MSCs, the results of growth status, reactive oxygen species (ROS) value, and lipid peroxidation were analyzed in BMMSCs and CBMSCs. We found that kinetin and EGCG treated MSCs expressed similar surface marker profiles with normal culture condition. The results also showed that the lower oxidative status, the higher catalase activity, and the longer lifespan could be found in kinetin cultivation than control condition in both types of MSCs. However, there were no obvious differences between MSCs cultures with or without EGCG addition. In differentiation test, the osteogenesis of CBMSCs could be enhanced by kinetin and the adipogenesis could be inhibited by EGCG in both types of MSCs. Moreover, kinetin but not EGCG decreased the anti-aging marker β- galactosidase in late passage of BMMSCs and CBMSCs. These results indicated that kinetin could be a useful antioxidant to reduce cell senescence during MSCs expansion.
Chiu, Wen-hui y 邱玟惠. "The Legal Aspects of the Umbilical Cord Blood Stem Cells and its Derivatives". Thesis, 2005. http://ndltd.ncl.edu.tw/handle/9hcate.
東吳大學
法律學系
93
Medical research and clinical practice throughout the latter half of the twentieth century brought human beings to the brink of curing many devastating diseases, especially the malignancies, that have plagued, the most promising one of which would be the umbilical cord stem cell related biotechnology. The umbilical cord blood stem cell gains prevailing advantages over bone marrow and fetal stem cell in both technical and ethical aspects, which makes the business community recognize that umbilical cord blood cell transplant technology could yield significant financial profit. These profit-making enterprises have established autologous umbilical cord blood storage services, inviting parents to bank their umbilical cord blood for possible future use all over the developed world. In Taiwan it has stimulated a ready market for blood storage, and several companies offering private storage and some charity community offering public umbilical cord blood banks now exist, which make the prevalence of umbilical cord blood stem cell storage rate estimated up to 4 per 100 births in this country. The umbilical cord stem cells, used to be regarded as delivery wastes, appear in itself legally a brand new issue both on personal rights and property rights which have never been well elaborated in the past. Disputes are certainly to arise since the umbilical cord blood stem cell so valuable a kind of substance, which urges us to pay more attention. This article elaborates the biological characteristics, clinical applications, and future scientific implications of stem cell, with special emphasis on the unique properties of umbilical cord blood stem cell, in the leading Chapter 2, which will help to make better understanding on differences between the umbilical cord blood stem cell and other stem cell origins. The status quo and the high prevalence on umbilical cord blood banking services, private or public, in Taiwan is briefly expounded in Chapter 3. The general concepts regarding personal rights of excised human organ and tissue are reviewed concisely in Chapter 4, and a novel canvass “personal nature” is introduced wherein to appropriately characterize an interim category transitionary from the property rights to personal rights. This article delimits precisely the demarcation line of “personal nature” and “personal rights” by examining the proposed formal and essential requirements. The ownership of the umbilical cord blood stem cell and its derivatives is discussed in Chapter 5, where the wrong classic concepts are fully presented. Meanwhile this article address that mothers have the entitlement to ownership of the umbilical cord blood stem cell by all the legal, anatomical, biological, and genetic aspects. Also this article holds that the derivative inducing process of umbilical cord blood stem cell is not kind of natural breeding as coded in Article 69 and 766 of Civil Code, but that of the processing work as coded in Article 784 of Civil Code. Chapter 6 of this article examined the validity of the clauses of umbilical cord blood deposit contracts. Lack of proper regulations is described in Chapter 7. Finally this article makes some proposition and advice for the umbilical blood banking community
Chiang, Wei-ching y 江尉靖. "Ethical Issues in Human Embryonic Stem Cells Research: the Morals Status of Embryos". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/qz9s72.
國立中央大學
哲學研究所碩士在職專班
97
The important ethical issues of the life right to life and morals status of the embryo are trickered by the fast progress of human embryonic stem cells research. These ethical disputes must be analyzed in greater details in order to make a proper judgment of the practice of embryonic stem cell research. Therefore, this thesis first gives an analysis of the terms and concepts involved in the debate. It presents Peter Singer’s argument against the thesis of sanctity of life and Mary Anne Warren’s criteria of personhood. Both are single criterion for moral status and criticized by the latter Warren as inadequate to cover the diverse field of moral status. Warren proposes a multi-criteria account of moral status, but offers insufficient status to embryos in accordance with our moral experience. A Confucian proposal is put forward to mitigate some of its pitfalls. According to Confucianism we are endowed with a natural concern of the sufferings of others. Thus, a person is a moral agent and must act morally according to her feeling towards the suffering of others. The moral status of embryo can thus be established by the moral expression of humanity from the moral agency so that it becomes sufficient and reasonable to place proper moral judgment in embryonic stem cells research. We express the hope that laws and regulations could be adjusted in time while new technology is innovated in embryonic stem cells research. At the same time, we also seek alterative methods for the development of stem cell lines to avoid the destruction of embryos in the process. Then the research of embryonic stem cells could promote the value and quality of human life.
Santos, Daniela Dias. "Analysis of outcome and cancer stem cells status in patients with resectable pancreatic adenocarcinoma". Doctoral thesis, 2016. http://hdl.handle.net/10362/17356.
Tsai, Chih-Chien y 蔡志謙. "Oct4 and Nanog directly maintain self-renewal and undifferentiated status in mesenchymal stem cells". Thesis, 2011. http://ndltd.ncl.edu.tw/handle/86603676559024247306.
國立陽明大學
藥理學研究所
99
Oct4 and Nanog are pluripotency genes, but their roles in adult stem cells are unclear. Here, increase in Oct4 and Nanog expression along with increased proliferation and differentiation potential but decreased spontaneous differentiation were observed in early passage (E), hypoxic culture (H) and p21 knockdown (p21KD) mesenchymal stem cells (MSCs) compared to late passage (L), normoxic culture (N) and scrambled shRNA-overexpressed (Scr) MSCs. Knockdown of Oct4 and Nanog in E, H and p21KD MSCs decreased proliferation and differentiation potential, and enhanced spontaneous differentiation, whereas overexpression of Oct4 and Nanog in L, N and Scr MSCs increased proliferation and differentiation potential, and suppressed spontaneous differentiation. Moreover, E, H and p21KD MSCs expressed higher Dnmt1 compared to L, N and Scr MSCs. Using 5 aza-cytidine, the potent DNA demethylating agent, decreased proliferation and differentiation potential, and enhanced spontaneous differentiation in E, H and p21KD MSCs. These data demonstrate the roles of Oct4 and Nanog in maintaining self-renewal and undifferentiated state in MSCs.
Liu, Jia-Heng y 劉家亨. "The Effect of Low Level Light Irradiation on Senescence Status of Bone Marrow Derived Mesenchymal Stem Cells". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/gr86ta.
中原大學
生物醫學工程研究所
105
The study used D-galactose (D-gal) treated bone marrow mesenchymal stem cells (MSCs) as an aging model, and aimed to explore the influence of low level light irradiation (LLLI) on anti-senescence of stem cells. We first determined cell viability, activity of senescence-associated- β-galactosidase (SA-β-gal), reactive oxygen species (ROS) and malondialdehyde (MDA) level on D-gal treated MSCs. SA-β-gal activity, ROS and MDA level were found to increase with increasing concentration of D-gal, whereas cell viability decreased. After red (630 nm) and near-infrared (850 nm) light irradiation, MDA concentration and SA-β-gal activity declined, whereas cell viability elevated as compared to the control group. The results suggest that D-gal treatment could induce senescence of MSCs via oxidative stress. LLLI could delay MSCs senescence by reducing oxidative stress. In addition, the effect of LLLI to MSCs senescence gene, p53, p21, p16 expression and osteogenic marker expression was tested. The results showed p53, p21, and p16 senescence gene expression increased when increasing D-gal concentration while it decreased after LLLI. In osteogenic marker expression, increasing D-gal concentration inhibited alkaline phosphatase (ALP) and calcium deposition expression. Although ALP expression was lower than control group, calcium deposition expression was higher than control group after LLLI. Overall, D-gal is able to induce MSCs senescence and reduces MSCs growing and osteogenic differentiation ability. LLLI treatment can effectively delay MSCs senescence and induced MSCs growing and osteogenic differentiation.
Liu, Chi-mou y 劉技謀. "Hyaluronan substratum holds placenta derived mesenchymal stem cells at a slow-cycling status and with multidrug resistant characteristics". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/71798803897410113128.
國立成功大學
生物科技研究所碩博士班
97
In vivo, postnatal stem cells are usually quiescent and drug resistant; they can be prompted to proliferate when triggered by proper signals. In vitro, however, stem cells propagate quickly and differentiate spontaneously. Therefore, holding stem cells in vitro with reduced growth and multidrug resistance (MDR) phenotype is an appropriate strategy for keeping them in a primitive state. Because hyaluronan has been recognized as a crucial regulator for maintaining the microenvironments termed stem cell niches, we examined whether hyaluronan induces slow cycling and drug resistance in placenta-derived mesenchymal stem cells (PDMSCs) by comparing hyaluronan-coated surface with tissue-culture polystyrene surface. The hyaluronan-coated surface significantly downregulated the proliferation of PDMSCs, more of which were maintained in the G0/G1 phases than were cells on the tissue-culture polystyrene surface. Both PKH-26 labeling and BrdU incorporation assays showed that most PDMSCs grown on a hyaluronan-coated surface duplicated during cultivation indicating that the hyaluronan-coated surface did not inhibit PDMSCs from entering the cell cycle. Mitotic synchronization showed that the G1-phase transit was prolonged in PDMSCs growing on a hyaluronan-coated surface. In addition, increases in p27Kip1 and p130 were the crucial factors that allowed hyaluronan to lengthen the G1 phase. We found that PDMSCs cultured on a tissue-culture polystyrene surface coated with 30 μg/cm2 hyaluronan were more resistant to doxorubicin compared to control PDMSCs. Inhibiting PI3-K/Akt signaling showed that the PI3-K/Akt pathway modulated both P-glycoprotein activity and doxorubicin resistance. In addition, 10 μM verapamil dramatically suppressed the doxorubicin resistance induced by the hyaluronan-coated surface, indicating that P-glycoprotein activity was necessary for MDR. We further demonstraterd that PDMSCs treated with CD44 small interfering RNA (siRNA) and grown on a polystyrene surface coated with 30 μg/cm2 hyaluronan had fewer P-glycoprotein+ cells and lower CD44 expression levels (less than 60% in both cases) compared with PDMSCs not treated with CD44 siRNA and grown on the hyaluronan-coated surface. Moreover, treatment with CD44 siRNA suppressed the hyaluronan-substratum-induced resistance of PDMSCs to doxorubicin. The data provided herein demonstrated that hyaluronan might be a promising candidate for maintaining stem cells in slow-cycling mode by prolonging their G1-phase transit. In addition, we presume that induction of MDR by hyaluronan would hold PDMSCs in a primitive state by providing the capability to extrude molecules required for differentiation. It seems reasonable to suggest that hyaluronan may cause PDMSCs to enter a program of dormancy, the natural state of stem cells consisting with slow cycling and drug resistance. Therefore, our findings may contribute to a deeper understanding of physiological functions of hyaluronan in stem cell research, and that is valuable for the applications in regenerative medicine.
Khand, Bishnu. "Studies on differentiation of mouse GS-2 ES-cells to pancreatic β-islet-like cells and their functional maturation status". Thesis, 2018. https://etd.iisc.ac.in/handle/2005/5392.
Hu, Chung-We y 胡中瑋. "Enactment of Technological Policies and Deliberative Democracy--Focusing on Legal Policies for The Research of Embryo Stem Cells in Taiwan". Thesis, 2008. http://ndltd.ncl.edu.tw/handle/98585453973263749289.
國立清華大學
科技法律研究所
96
Technological policies have long been influenced by experts. Since the threshold is extremely high for knowledge of technology, it is difficult for the layman to participate in enacting technological policies and to challenge the authority of science experts. Under this kind of frameworks, the common people don’t possess sufficient knowledge to substantially participate in technological decision-making. Therefore, policies determined by experts easily fall outside of the boundaries of democratic politics. By the experience of Missouri Stem Cell Research and Cures Initiative, this thesis introduced the values and functions of “Deliberative Democracy” and its association with technological communication. In contemplation of the experience from Missouri Stem Cell Research and Cures Initiative, this thesis tried to bring in citizen engagement in the procedures of prospective embryo stem cells’ referendum in Taiwan. This would enable the multitude to engage in the public domain of communication and debate, and to make collective decisions approximate to the shared good and in accordance with social justice principles. Hence, it could function to mend the weakening of congressional legislation and further more to enhance the justification of technological policies.
Adusumilli, Vijaya. "Investigating the role of cell-autonomous ROS status in the regulation of hippocampal neural precursor cells in adult mice". 2020. https://tud.qucosa.de/id/qucosa%3A72802.
Tien, Su-Liang y 田素良. "Study on the Attitude and Policies of the Ministry ofHealth of Taiwan Government to the Legal ClinicalAppliance and Treatments by the Stem Cells". Thesis, 2011. http://ndltd.ncl.edu.tw/handle/45947038977040489942.
國立臺灣大學
健康政策與管理研究所
99
The Clinical appliance of Stem Cells started from 8th century, meanwhile the Research went to a new mile stone since from 19th century. The continues research combine with lots of experts, clinicians, professors, technologists of Clinical Medicine, Basic Medicine, Medical Technology, Pharmacology, Dentistry, Material Engineering, Molecular Biology, BioTechnology, Bio Medicine of different fields from different countries has been developing day after day. Modern Technology of Stem Cells widely use Adult Autologus, Allogeneous, Xenogeneous Stem Cells add the mature technique of HLA-typing and the well understanding of GvHD which caused by Allogeneous, Xenogeneous Stem Cells implantation, More and more large scaled animal or Human Clinical Trials were successfully practiced under using the getting matured technique of Genes transplantation,combined therapy of Stem Cells and or Tissue Engineering of Stem Cells. More and more news were broadcasted in front of people which revealed many diseases which could not be cured under so called Current Medical Care werecured ,getting improved, recovered under the aid of Stem Cells .The heart of people full filled of hope to Stem Cells. We must have the vi combination of reasonable and sufficient laws, rulings ,and policies to make Taiwan as the island of Bio technology while the government of Taiwan develop Bio technology strongly. Whether the IT industry will be replaced by the industry of Bio technology which make Taiwan become the island of Bio technology industry, and it depend on the orientations and policies of the government as the developing of Stem Cells will be listed as the focus and superior or rewarded item of Biotechnology. The Research use the Qualitative Researching methods which select many interviewers belong to certain manufacturing ,government ,and professions to interviewed by the methods of In-depth interview. Finally the conclusion was made according to the analysis of the research. We do the cross comparison and analysis of the interviewers without the classification of their occupation of the research . The generalized conclusion of the research as like the following items: they are (1). If the Stem Cells Research will become one part of Main stremed Medicine? Yes: 83.4 %, No: 0% ,Not sure: 16.6%. (2). Wheather Autologus or Allogeneous Stem Cells will become the Main Streamed Ste, Cells Research? Autologus:50 %, Allogeneous:50%.(3).(3.1).The standard of Taiwan Stem Cells Research ? Has reached the international standard: 0%, Not yet reached the international standard: 100%,Not sure: 0%.(3.2)Does Taiwan need import the advanced Stem Cells technique overseas? Yes:100%,No:0%,Not sure: 0%. (4).(4.1) Concepts to the Umbilical Cord Blood vii Storage in Taiwan? Meaningful:0%, Not too meaningful:100%, Not clear:0%.(4.2).Futuring of the Umbilical Cord Blood storage in Taiwan ? Good futuring: 0%,Not good futuring: 100%, Not clear:0%. (5).Imported International technique of Adult Stem Cells of storage and treatment to Taiwan? Need: 100%,No need:0%,not clear:0%/. (6).What orientation should be adopted to the Stem Cells policies? Opened under certain qualifications: 100%, Policies should be restricted:0%, no comments: 0%. (7).(7.1)Should Taiwan government treat Bio-technique industries as the first priority of the government policies ? Yes: 83.4%, No:0%,not clear: 16.6%. (7.2) Should Taiwan government treat and reward the research of Stem Cells as the first priority of her policies? Yes: 41.55%, No: 8.7%, not sure: 49.8%. (8). Do you agree with adoption Stem Cells treatment to the payment of generalized health insurance in Taiwan? Yes: 83.34, No: 0%, Yes under certain situations: 16.66%. Hopefully, the result can be treated as the main resource to the related officials, decisions makers, certain persons of professions of medical, Bio medical, Stem cells research, medical policies, related fields of industries.
BŘEZINOVÁ, Nicola. "Pohled na statut lidského embrya z filosoficko-etického hlediska". Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-381802.