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Artículos de revistas sobre el tema "Laser capture microdissection"

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Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 20 de febrero de 2023

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1

Emmert-Buck, Michael R., Robert F. Bonner, Paul D. Smith, Rodrigo F. Chuaqui, Zhengping Zhuang, Seth R. Goldstein, Rhonda A. Weiss y Lance A. Liotta. "Laser Capture Microdissection". Science 274, n.º 5289 (8 de noviembre de 1996): 998–1001. http://dx.doi.org/10.1126/science.274.5289.998.

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2

Espina, Virginia, Julia D. Wulfkuhle, Valerie S. Calvert, Amy VanMeter, Weidong Zhou, George Coukos, David H. Geho, Emanuel F. Petricoin y Lance A. Liotta. "Laser-capture microdissection". Nature Protocols 1, n.º 2 (27 de junio de 2006): 586–603. http://dx.doi.org/10.1038/nprot.2006.85.

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3

Tresser, N., M. Ouezado, L. Whitney, K. Becker, R. Bonner, M. Emmert-Buck y L. Liotta. "LASER CAPTURE MICRODISSECTION". Journal of Neuropathology and Experimental Neurology 57, n.º 5 (mayo de 1998): 505. http://dx.doi.org/10.1097/00005072-199805000-00164.

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4

Jensen, Ellen. "Laser-Capture Microdissection". Anatomical Record 296, n.º 11 (4 de octubre de 2013): 1683–87. http://dx.doi.org/10.1002/ar.22791.

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5

Grant, Kenneth y W. Gray Jerome. "Laser Capture Microdissection as an Aid to Ultrastructural Analysis". Microscopy and Microanalysis 8, n.º 3 (junio de 2002): 170–75. http://dx.doi.org/10.1017/s143192760202010x.

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Laser capture microdissection uses a microscope to identify specific cells for microdissection and then a laser-sensitive plastic to capture and remove the cells from their substrate. This efficient capture method was originally developed to capture cells for genetic analysis. However, it has also been used to capture cells for proteonomic analysis. In this article, we extend the uses of laser-capture microdissection by reporting a method for preparing captured cells for ultrastructural analysis by transmission electron microscopy. Cells prepared by our methodology show good fine structure preservation and are easily sectioned by standard ultramicrotomy.
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6

Espina, Virginia, Michael Heiby, Mariaelena Pierobon y Lance A. Liotta. "Laser capture microdissection technology". Expert Review of Molecular Diagnostics 7, n.º 5 (septiembre de 2007): 647–57. http://dx.doi.org/10.1586/14737159.7.5.647.

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7

Chokechanachaisakul, Uraiwan, Tomoatsu Kaneko, Takashi Okiji, Reika Kaneko, Hideaki Suda y Jacques E. Nör. "Laser Capture Microdissection in Dentistry". International Journal of Dentistry 2010 (2010): 1–8. http://dx.doi.org/10.1155/2010/592694.

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Laser capture microdissection (LCM) allows for the microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. According to the recent development of the LCM technologies and methodologies, the LCM has been used in various kinds of tissue specimens in dental research. For example, the real-time polymerase-chain reaction (PCR) can be performed from the formaldehyde-fixed, paraffin-embedded, and immunostained sections. Thus, the advance of immuno-LCM method allows us to improve the validity of molecular biological analysis and to get more accurate diagnosis in pathological field in contrast to conventional LCM. This paper is focused on the presentation and discussion of the existing literature that covers the fields of RNA analysis following LCM in dentistry.
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8

Fend, F. "Laser capture microdissection in pathology". Journal of Clinical Pathology 53, n.º 9 (1 de septiembre de 2000): 666–72. http://dx.doi.org/10.1136/jcp.53.9.666.

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9

Fend, Falko, Marcus Kremer y Leticia Quintanilla-Martinez. "Laser Capture Microdissection: Methodical Aspects and Applications with Emphasis on Immuno-Laser Capture Microdissection". Pathobiology 68, n.º 4-5 (2000): 209–14. http://dx.doi.org/10.1159/000055925.

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10

Sujatha, Govindarajan y Jayanandan Muruganandhan. "Laser Capture Microdissection in Oral Cancer". Journal of Contemporary Dental Practice 19, n.º 5 (2018): 475–76. http://dx.doi.org/10.5005/jp-journals-10024-2286.

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11

Lechpammer, Mirna. "LASER CAPTURE MICRODISSECTION: METHODS AND PROTOCOLS". Shock 25, n.º 4 (abril de 2006): 426–27. http://dx.doi.org/10.1097/01.shk.0000223839.51394.1c.

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12

Goldstein, Seth R., Philip G. McQueen y Robert F. Bonner. "Thermal modeling of Laser Capture Microdissection". Applied Optics 37, n.º 31 (1 de noviembre de 1998): 7378. http://dx.doi.org/10.1364/ao.37.007378.

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13

Santegoeds, R. G. C., Y. Yakkioui, A. Jahanshahi, G. Raven, J. J. Van Overbeeke, A. Herrler y Y. Temel. "Notochord isolation using laser capture microdissection". Journal of Chemical Neuroanatomy 80 (marzo de 2017): 37–43. http://dx.doi.org/10.1016/j.jchemneu.2016.12.004.

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14

Heath, P. "Laser Capture Microdissection: Methods and Protocols". Histopathology 48, n.º 4 (marzo de 2006): 462. http://dx.doi.org/10.1111/j.1365-2559.2006.02262.x.

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15

Srinivasan, Shanthi y Shadi Sadeghi Yarandi. "Laser Capture Microdissection: Methods and Protocols". Gastroenterology 142, n.º 5 (mayo de 2012): 1256. http://dx.doi.org/10.1053/j.gastro.2012.03.025.

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16

Korabecna, Marie, Zbynek Tonar, Zoltan Tomori y Erna Demjen. "Optimized cutting laser trajectory for laser capture microdissection". Biologia 74, n.º 6 (3 de abril de 2019): 717–24. http://dx.doi.org/10.2478/s11756-019-00234-x.

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17

Hunt, Jennifer L. y Sydney D. Finkelstein. "Microdissection Techniques for Molecular Testing in Surgical Pathology". Archives of Pathology & Laboratory Medicine 128, n.º 12 (1 de diciembre de 2004): 1372–78. http://dx.doi.org/10.5858/2004-128-1372-mtfmti.

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Abstract Objective.—To describe the techniques for microdissection of paraffin-embedded and frozen tissue sections for the use in molecular applications. Data Sources.—Original research papers and review papers and the authors' personal experiences. Data Synthesis.—Manual and laser-capture microdissection are described in detail, with specific protocols for sample preparation and instructions for performing the microdissection. A section addressing frequently asked questions is also included. Conclusions.—Microdissection is a technique that is very useful both in the research setting and for clinical molecular testing in paraffin-embedded tissue samples. The available techniques range from simple and inexpensive (manual microdissection) to complex and expensive (laser-capture microdissection). All of the techniques, however, require the user to be familiar with microscopy and histology.
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18

Ellsworth, D. L., C. D. Shriver, R. E. Ellsworth, B. Deyarmin y R. I. Somiari. "Laser Capture Microdissection of Paraffin-Embedded Tissues". BioTechniques 34, n.º 1 (enero de 2003): 42–46. http://dx.doi.org/10.2144/03341bm05.

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19

Todd, Randy y Mark W. Lingenand Winston P. Kuo. "Gene expression profiling using laser capture microdissection". Expert Review of Molecular Diagnostics 2, n.º 5 (septiembre de 2002): 497–507. http://dx.doi.org/10.1586/14737159.2.5.497.

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20

Liu, H., T. L. McDowell, N. E. Hanson, X. Tang, J. Fujimoto y J. Rodriguez-Canales. "Laser Capture Microdissection for the Investigative Pathologist". Veterinary Pathology 51, n.º 1 (13 de noviembre de 2013): 257–69. http://dx.doi.org/10.1177/0300985813510533.

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21

Webb, T. "Laser Capture Microdissection Comes Into Mainstream Use". Journal of the National Cancer Institute 92, n.º 21 (1 de noviembre de 2000): 1710–11. http://dx.doi.org/10.1093/jnci/92.21.1710.

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22

Vandewoestyne, Mado y Dieter Deforce. "Laser capture microdissection for forensic DNA analysis". Forensic Science International: Genetics Supplement Series 3, n.º 1 (diciembre de 2011): e117-e118. http://dx.doi.org/10.1016/j.fsigss.2011.08.058.

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23

Kuhn, Donald E., Sashwati Roy, Jared Radtke, Sudip Gupta y Chandan K. Sen. "Laser microdissection and pressure-catapulting technique to study gene expression in the reoxygenated myocardium". American Journal of Physiology-Heart and Circulatory Physiology 290, n.º 6 (junio de 2006): H2625—H2632. http://dx.doi.org/10.1152/ajpheart.01346.2005.

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For focal events such as myocardial infarction, it is important to dissect infarction-induced biological responses as a function of space with respect to the infarct core. Laser microdissection pressure catapulting (LMPC) represents a recent variant of laser capture microdissection that enables robot-assisted rapid capture of catapulted tissue without direct user contact. This work represents the maiden effort to apply laser capture microdissection to study spatially resolved biological responses in myocardial infarction. Infarcted areas of the surviving ischemic-reperfused murine heart were identified using a standardized hematoxylin QS staining procedure. Standard staining techniques fail to preserve tissue RNA. Exposure of the tissue to an aqueous medium (typically used during standard immunohistochemical staining), with or without RNase inhibitors, resulted in a rapid degradation of genes, with ∼80% loss in the 1st h. Tissue elements (1 × 104–4 × 106 μm2) captured from infarcted and noninfarcted sites with micrometer-level surgical precision were collected in a chaotropic RNA lysis solution. Isolated RNA was analyzed for quality by microfluidics technology and reverse transcribed to generate high-quality cDNA. Real-time PCR analysis of the cDNA showed marked (200- and 400-fold, respectively) induction of collagen Ia and IIIa at the infarcted site compared with the noninfarcted site. This work reports a sophisticated yet rapid approach to measurement of relative gene expressions from tissue elements captured from spatially resolved microscopic regions in the heart with micrometer-level precision.
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24

Blatt, Richard y Shanthi Srinivasan. "Defining Disease With Laser Precision: Laser Capture Microdissection in Gastroenterology". Gastroenterology 135, n.º 2 (agosto de 2008): 364–69. http://dx.doi.org/10.1053/j.gastro.2008.06.054.

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25

SIMONE, NICOLE L., CLOUD P. PAWELETZ, LU CHARBONEAU, EMANUEL F. PETRICOIN y LANCE A. LIOTTA. "Laser Capture Microdissection: Beyond Functional Genomics to Proteomics". Molecular Diagnosis 5, n.º 4 (2000): 301–7. http://dx.doi.org/10.2165/00066982-200005040-00008.

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26

Semere, Tesfakiros y Atul Kumar. "Laser capture microdissection and its applications in plants". Journal of Hill Agriculture 7, n.º 2 (2016): 173. http://dx.doi.org/10.5958/2230-7338.2016.00041.0.

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27

Yan, J. S. "Book Review: Laser Capture Microdissection Methods and Protocols". Veterinary Pathology 42, n.º 5 (septiembre de 2005): 675. http://dx.doi.org/10.1354/vp.42-5-675.

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28

Simone, N. "Laser capture microdissection: Beyond functional genomics to proteomics". Molecular Diagnosis 5, n.º 4 (diciembre de 2000): 301–7. http://dx.doi.org/10.1054/modi.2000.19808a.

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29

Banks, R. E., M. J. Dunn, M. A. Forbes, A. Stanley, D. Pappin, T. Naven, M. Gough, P. Harnden y P. J. Selby. "LASER CAPTURE MICRODISSECTION AND PROTEOMIC ANALYSIS — PRELIMINARY FINDINGS". Biochemical Society Transactions 27, n.º 3 (1 de junio de 1999): A67. http://dx.doi.org/10.1042/bst027a067c.

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30

Kerk, Nancy M., Teresa Ceserani, S. Lorraine Tausta, Ian M. Sussex y Timothy M. Nelson. "Laser Capture Microdissection of Cells from Plant Tissues". Plant Physiology 132, n.º 1 (1 de mayo de 2003): 27–35. http://dx.doi.org/10.1104/pp.102.018127.

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31

Jin, Long, Katharina H. Ruebel, Jill M. Bayliss, Ikuo Kobayashi y Ricardo V. Lloyd. "Immunophenotyping Combined with Laser Capture Microdissection (Immuno-LCM)". ACTA HISTOCHEMICA ET CYTOCHEMICA 36, n.º 1 (2003): 9–13. http://dx.doi.org/10.1267/ahc.36.9.

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32

Dundas, Sinclair R., Stephanie Curran y Graeme I. Murray. "Laser Capture Microdissection: Applications in Urological Cancer Research". UroOncology 2, n.º 1 (enero de 2002): 33–35. http://dx.doi.org/10.1080/15610950290013530.

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33

Yazdi, Amir S., Ursula Puchta, Michael J. Flaig y Christian A. Sander. "Laser-capture microdissection: Applications in routine molecular dermatopathology". Journal of Cutaneous Pathology 31, n.º 7 (6 de julio de 2004): 465–70. http://dx.doi.org/10.1111/j.0303-6987.2004.00221.x.

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34

Vandewoestyne, Mado, David Van Hoofstat, Filip Van Nieuwerburgh y Dieter Deforce. "Automatic detection of spermatozoa for laser capture microdissection". International Journal of Legal Medicine 123, n.º 2 (26 de julio de 2008): 169–75. http://dx.doi.org/10.1007/s00414-008-0271-1.

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35

Vandewoestyne, Mado y Dieter Deforce. "Laser capture microdissection in forensic research: a review". International Journal of Legal Medicine 124, n.º 6 (3 de agosto de 2010): 513–21. http://dx.doi.org/10.1007/s00414-010-0499-4.

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36

Lin, Juan, Gabrielle Marquardt, Nandita Mullapudi, Tao Wang, Weiguo Han, Miao Shi, Steven Keller, Changcheng Zhu, Joseph Locker y Simon D. Spivack. "Lung Cancer Transcriptomes Refined with Laser Capture Microdissection". American Journal of Pathology 184, n.º 11 (noviembre de 2014): 2868–84. http://dx.doi.org/10.1016/j.ajpath.2014.06.028.

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37

Craven, Rachel A. y Rosamonde E. Banks. "Laser capture microdissection and proteomics: Possibilities and limitation". PROTEOMICS 1, n.º 10 (octubre de 2001): 1200–1204. http://dx.doi.org/10.1002/1615-9861(200110)1:10<1200::aid-prot1200>3.0.co;2-q.

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38

Park, Emily S., Justin P. Yan, Richard A. Ang, Jeong Hyun Lee, Xiaoyan Deng, Simon P. Duffy, Kevin Beja et al. "Isolation and genome sequencing of individual circulating tumor cells using hydrogel encapsulation and laser capture microdissection". Lab on a Chip 18, n.º 12 (2018): 1736–49. http://dx.doi.org/10.1039/c8lc00184g.

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39

Vandewoestyne, Mado, Karen Goossens, Christian Burvenich, Ann Van Soom, Luc Peelman y Dieter Deforce. "Laser capture microdissection: Should an ultraviolet or infrared laser be used?" Analytical Biochemistry 439, n.º 2 (agosto de 2013): 88–98. http://dx.doi.org/10.1016/j.ab.2013.04.023.

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40

Chand, A. L., A. Murray, R. L. Jones, L. Salamonsen y L. Rombauts. "250.Chemokine profiling in endometriosis using laser capture microdissection". Reproduction, Fertility and Development 16, n.º 9 (2004): 250. http://dx.doi.org/10.1071/srb04abs250.

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Endometriosis is an inflammatory condition with elevated leukocyte infiltrate; defined as the ectopic growth of endometrium-like tissue, characterised by epithelial glands, outside the uterus. Chemokines selectively regulate influx and activation of leukocyte subpopulations. Their non-immune functions during tissue remodelling and disease pathogenesis include up-regulation of adhesion molecules, stimulation of inflammatory mediators, angiogenesis, cell proliferation and motility (1). The aims of the present study were to compare the chemokine mRNA profiles expressed by the epithelial glands of: eutopic endometrium from patients with / without endometriosis. Tissue heterogeneity in the endometrium and in ectopic lesions hinders precise study of the contribution of cell-specific inflammatory responses. Laser Capture Microdissection (LCM) was therefore utilised. Frozen eutopic curettings and ectopic endometriosis lesions were sectioned, H&E stained and glandular epithelium laser captured. 327 captured glands yields approximately 27 ng of RNA from each endometrial sample. To obtain enough RNA for gene array analysis and verification studies, cellular mRNA was amplified. Two rounds of linear mRNA amplification provided a sufficient yield of >1.8 μg from 1 ng of RNA. RNA from 4 patients and 4 controls were pooled, amplified and probed on gene arrays to build a chemokine profile. We identified 45 chemokines / receptors that are specifically abundant in glandular epithelium. 39 were highly upregulated (2- to 60-fold) in women with endometriosis compared to controls. In contrast, only 2 genes were downregulated more than 2-fold in endometriosis patients. Most genes have not been previously studied for their roles in endometriosis. Verification studies are currently being carried out. This is a novel study providing evidence of a distinct profile of the chemokine/receptors collectively in women with endometriosis. (1) J. Immunol. (2002) 168, 4301.
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41

da Costa, Katiane de Almeida, Helena Malvezzi, Bruno Gallani Viana, Renée Zon Filippi, Rosa Maria Neme, Thiago Pinheiro Arrais Aloia, Sérgio Podgaec y Carla de Azevedo Piccinato. "Validation of Laser Capture Microdissection Protocol in Endometriosis Studies". Medicina 55, n.º 9 (22 de agosto de 2019): 520. http://dx.doi.org/10.3390/medicina55090520.

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Background and Objectives: The presence of endometrial-like tissue outside the uterine cavity is a key feature of endometriosis. Although endometriotic lesions appear to be histologically quite similar to the eutopic endometrium, detailed studies comparing both tissues are required because their inner and surrounding cellular arrangement is distinct. Thus, comparison between tissues might require methods, such as laser capture microdissection (LCM), that allow for precise selection of an area and its specific cell populations. However, it is known that the efficient use of LCM depends on the type of studied tissue and on the choice of an adequate protocol. Recent studies have reported the use of LCM in endometriosis studies. The main objective of the present study is to establish a standardized protocol to obtain good-quality microdissected material from eutopic or ectopic endometrium. Materials and Methods: The main methodological steps involved in the processing of the lesion samples for LCM were standardized to yield material of good quality to be further used in molecular techniques. Results: We obtained satisfactory results regarding the yields and integrity of RNA and protein obtained from LCM-processed endometriosis tissues. Conclusion: LCM can provide more precise analysis of endometriosis biopsies, provided that key steps of the methodology are followed.
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42

Seminati, Davide, Gabriele Casati, Fabio Pagni y Filippo Fraggetta. "Laser capture microdissection in lung cancer: a narrative review". Journal of Xiangya Medicine 7 (marzo de 2022): 8. http://dx.doi.org/10.21037/jxym-21-55.

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43

Best, Carolyn JM y Michael R. Emmert-Buck. "Molecular profiling of tissue samples using laser capture microdissection". Expert Review of Molecular Diagnostics 1, n.º 1 (mayo de 2001): 53–60. http://dx.doi.org/10.1586/14737159.1.1.53.

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44

Suarez-Quian, C. A., S. R. Goldstein, T. Pohida, P. D. Smith, J. I. Peterson, E. Wellner, M. Ghany y R. F. Bonner. "Laser Capture Microdissection of Single Cells from Complex Tissues". BioTechniques 26, n.º 2 (febrero de 1999): 328–35. http://dx.doi.org/10.2144/99262rr03.

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45

Bonner, R. F. "CELL SAMPLING: Laser Capture Microdissection: Molecular Analysis of Tissue". Science 278, n.º 5342 (21 de noviembre de 1997): 1481–83. http://dx.doi.org/10.1126/science.278.5342.1481.

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46

Roy Chowdhuri, Sinchita, Jeffrey Hanson, Jerome Cheng, Jaime Rodriguez-Canales, Patricia Fetsch, Ulysses Balis, Armando C. Filie, Giuseppe Giaccone, Michael R. Emmert-Buck y Jason D. Hipp. "Semiautomated Laser Capture Microdissection of Lung Adenocarcinoma Cytology Samples". Acta Cytologica 56, n.º 6 (2012): 622–31. http://dx.doi.org/10.1159/000342984.

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47

Craven, Rachel A., Nick Totty, Patricia Harnden, Peter J. Selby y Rosamonde E. Banks. "Laser Capture Microdissection and Two-Dimensional Polyacrylamide Gel Electrophoresis". American Journal of Pathology 160, n.º 3 (marzo de 2002): 815–22. http://dx.doi.org/10.1016/s0002-9440(10)64904-8.

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48

Carmichael, Stephen W. "Laser Capture Microdissection: A Punch Biopsy Under the Microscope". Microscopy Today 6, n.º 1 (enero de 1998): 3–4. http://dx.doi.org/10.1017/s1551929500059009.

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The size and precision af a tissue sample has always been a concern. As it has become possible to analyze smaller and smaller samples with molecular techniques, one must be increasingly concerned that the sample is what we think it is. So how do you dissect out a specific cell or a small group of cells? Whereas several techniques have been suggested, a group at the National Institutes of Health (NIH) have come up with an ingenious solution. Robert Bonner, Michael Emmert-Buck, Kristina Cole, Thomas Pohida, Rodrigo Chuaqui, Seth Goldstein, and Lance Liotta have published a technique they have termed Laser Capture Microdissection (LCM).With LCM, Bonner et. al. have demonstrated that a specific cell can be identified and transferred to an appropriate container for molecular analysis. Using the polymerase chain reaction (PCR), the genetic material from a single cell can be amplified for analysis. However, data from several cells (about 20) needs to be examined so that variations in the cell cycle, etc., can be averaged.
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49

Anslinger, K., B. Mack, B. Bayer, B. Rolf y W. Eisenmenger. "Digoxigenin labelling and laser capture microdissection of male cells". International Journal of Legal Medicine 119, n.º 6 (5 de febrero de 2005): 374–77. http://dx.doi.org/10.1007/s00414-005-0523-2.

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50

Traslavina, R. P., C. M. Reilly, R. Vasireddy, E. M. Samitz, C. T. Stepnik, C. Outerbridge, V. K. Affolter et al. "Laser Capture Microdissection of FelineStreptomyces sppPyogranulomatous Dermatitis and Cellulitis". Veterinary Pathology 52, n.º 6 (16 de diciembre de 2014): 1172–75. http://dx.doi.org/10.1177/0300985814561094.

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