Tesis sobre el tema "Lactic acid – Metabolism"
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Newbold, Charles James. "Microbial metabolism of lactic acid in the rumen". Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235529.
Texto completoLee, R. J. "Lactic acid metabolism and lactate dehydrogenases of Vibrio species". Thesis, University of Portsmouth, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377561.
Texto completoBourniquel, Aude A. "Molecular insights into the metabolism and physiology of the lactic acid basterium "Lactobacillus delbrueckii" subsp. "lactis"". Basel : Universität Basel, 2000. http://www.unibas.ch/diss/2000/DissB_6242.htm.
Texto completoFurumoto, Hidehiro. "Studies on Nutraceutical Properties of Modified Fatty Acids by Autoxidation and Lactic Acid Bacterial Metabolism". Kyoto University, 2016. http://hdl.handle.net/2433/215592.
Texto completo0048
新制・課程博士
博士(農学)
甲第19766号
農博第2162号
新制||農||1040(附属図書館)
学位論文||H28||N4982(農学部図書室)
32802
京都大学大学院農学研究科応用生物科学専攻
(主査)教授 菅原 達也, 教授 澤山 茂樹, 教授 佐藤 健司
学位規則第4条第1項該当
Takeuchi, Michiki. "Biochemical and applied studies on unsaturated fatty acid metabolisms in lactic acid bacteria". Kyoto University, 2015. http://hdl.handle.net/2433/199370.
Texto completo0048
新制・課程博士
博士(農学)
甲第19046号
農博第2124号
新制||農||1032(附属図書館)
学位論文||H27||N4928(農学部図書室)
31997
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 小川 順, 教授 加納 健司, 教授 植田 充美
学位規則第4条第1項該当
Loat, Christopher Eino Russell. "Comparison of the lactate and ventilatory thresholds during prolonged work". Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/30153.
Texto completoEducation, Faculty of
Curriculum and Pedagogy (EDCP), Department of
Graduate
Whitley, Katherine. "Phenotypic variants of lactic acid bacteria, their metabolism and relevance to probiotic criteria". Thesis, University of Huddersfield, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323780.
Texto completoYamazaki, Shinichi. "Bioelectrochemical analysis on quinone-induced modification of the metabolism in bifidobacteria and lactic acid bacteria". Kyoto University, 2002. http://hdl.handle.net/2433/149899.
Texto completo0048
新制・課程博士
博士(農学)
甲第9608号
農博第1236号
新制||農||841(附属図書館)
学位論文||H14||N3640(農学部図書室)
UT51-2002-G366
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 池田 篤治, 教授 清水 昌, 教授 加藤 暢夫
学位規則第4条第1項該当
Weber, Jean-Michel. "Lactate turnover in fast-moving vertebrates : the control of plasma metabolite fluxes". Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/27561.
Texto completoScience, Faculty of
Zoology, Department of
Graduate
Vukovich, Matthew D. "Effect of L-carnitine supplementation on muscle glycogen utilization and lactate accumulation during cycle exercise". Virtual Press, 1993. http://liblink.bsu.edu/uhtbin/catkey/862276.
Texto completoHuman Performance Laboratory
Taylor, Melarie Ellen. "Glucose and lactic acid metabolism of the dog in prolonged cardiogenic shock due to pericardial tamponade". Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=65383.
Texto completoBuyukkileci, Ali Oguz. "Investigation Of Sugar Metabolism In Rhizopus Oryzae". Phd thesis, METU, 2007. http://etd.lib.metu.edu.tr/upload/12608622/index.pdf.
Texto completoHoncharenko, Iryna. "The role of lactates in medicine". Thesis, National Aviation University, 2021. https://er.nau.edu.ua/handle/NAU/50626.
Texto completoLactate, or lactic acid (2-hydroxypropanoic acid), is a normal product of metabolism and is formed from pyruvic acid under anaerobic conditions in skeletal muscle tissue (25%), brain (20%), skin (25%), erythrocytes (20 %), leukocytes, mucous membrane of the small intestine and the renal medulla. Preferably lactate is metabolized in the liver (60%), kidneys (30%) and only a small amount is utilized in other tissues (10%). Measuring blood lactate levels is especially important in cases of severe sepsis and septic shock. In this context, lactate is commonly used to assess the severity of the disease, analyze the response to treatment and further prognosis. Hypoperfusion, which is most often associated with blood loss, is observed in patients with trauma. Elevated lactate levels may help to identify patients in whom normal vital signs are initially masked by tissue hypoperfusion. In patients with cardiogenic shock who require extracorporeal membrane oxygenation, lactate is a useful parameter for predicting mortality. Lactate levels increase during strenuous exercise, mainly due to anaerobic glucose metabolism. A common feature of primary and metastatic cancer is an increase in the rate of glycolysis, which leads to increased glucose uptake and lactate formation even under normal oxygen levels. Лактат або молочна кислота (2-гідроксипропанова кислота), є нормальним продуктом обміну речовин і утворюється з піровиноградної кислоти при анаеробних умовах у тканині скелетних м'язів (25%), мозку (20%), шкіри (25%), еритроцити (20%), шкіри (25%), еритроцити (20%), 20%), лейкоцити, слизової оболонки тонкого кишечника та ниркової медулі. Переважно лактат метаболізується в печінці (60%), нирках (30%), і лише невелика кількість використовуються в інших тканинах (10%). Вимірювання рівнів лактату крові особливо важлива у випадках важкого сепсису та септичного шоку. У цьому контексті, лактат зазвичай використовується для оцінки тяжкості захворювання, аналізують реакцію на лікування та подальший прогноз. Гіпоперфузія, яка найчастіше пов'язана з крововтратою, спостерігається у пацієнтів з травмою. Підвищений рівень лактатів може допомогти виявити пацієнтів, у яких звичайні життєві ознаки спочатку маскують гіпоперфузією тканин. У пацієнтів з кардіогенним шоком, які потребують екстракорпоральної мембранної оксигенації, лактат є корисним параметром для прогнозування смертності. Lactate рівень збільшується під час напружених вправ, головним чином завдяки анаеробному метаболізму глюкози.
Wender, Regina. "Astrocyte-axon interactions in central white matter energy metabolism : the roles of glycogen and lactate /". Thesis, Connect to this title online; UW restricted, 2000. http://hdl.handle.net/1773/10536.
Texto completoBarnett, Christopher. "The effect of L-carnitine supplementation on blood and muscle lactate accumulation during high intensity sprint cycling exercise". Virtual Press, 1993. http://liblink.bsu.edu/uhtbin/catkey/865941.
Texto completoSchool of Physical Education
竹内, 道樹. "乳酸菌の不飽和脂肪酸代謝に関する生化学的研究とその応用". Kyoto University, 2015. http://hdl.handle.net/2433/199538.
Texto completoCanon, Fanny. "Favoriser les interactions positives entre bactéries lactiques : vers de nouvelles applications alimentaires". Thesis, Rennes, Agrocampus Ouest, 2021. http://www.theses.fr/2021NSARB351.
Texto completoThe adage “in unity is strength” also applies to fermented foods: the association of lactic acid bacteria (LAB) strains brings benefits to their organoleptic properties, sanitary quality and potential health benefits. These functionalities can depend on positive interactions, as observed between LAB in yogurt. Finding ways to create new functional associations of LAB strains is challenging, especially in the context of the food transition where new resources, especially in proteins, are increasingly used. The objective of this work was to understand how to promote positive interactions between LAB, on the basis of their nitrogen metabolism, to conceive new food applications. The interactions were studied in cocultures that associated a proteolytic (prot+) and a non- proteolytic (prot-) strain. These prot+/prot- pairs were first grown in a model medium containing milk and lupinproteins as sole nitrogen nutriments, in which the growth of prot- strain thus depended on the peptides and amino acids released by the prot+ strain. Strong, weak, or no positive interactions were observed between prot+ strains and prot- strains. Our results showed that the release of branched-chain amino acids in their free form or within peptides was at the heart of the positive interactions observed. The impact of these interactions was then studied in alternative yogurts made from milk and lupin, characterized for their physical and sensory properties. The positive interactions between LAB lead to diversified functionalities of alternative yogurts, such as flavor compound and organic acid formation and texture improvement. This work brings new knowledge on the interactions between LAB, which is useful in designing future fermented foods
Kassem, Milad Verfasser], Wolfgang [Akademischer Betreuer] Liebl, Rolf [Akademischer Betreuer] [Daniel, Thoma [Akademischer Betreuer] Friedl y Michael [Akademischer Betreuer] Hoppert. "Investigation of storage polysaccharide metabolism in lactic acid bacteria / Milad Kassem. Gutachter: Rolf Daniel ; Thoma Friedl ; Michael Hoppert. Betreuer: Wolfgang Liebl". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2011. http://d-nb.info/1042641412/34.
Texto completoCaslin, Heather. "Glycolytic ATP production is required for innate mast cell activation and is limited by lactic acid, which effectively reduces LPS-induced cytokine production in mast cells and in vivo". VCU Scholars Compass, 2018. https://scholarscompass.vcu.edu/etd/5383.
Texto completoHutton, Peter. "Antimicrobial plants of Australia have the potential to prevent lactic acidosis in ruminants". University of Western Australia. School of Animal Biology, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0159.
Texto completoRACIOPPO, ANGELA. "Evaluation of the effects of alternative physical approach on the metabolism and functional traits of useful microorganisms". Doctoral thesis, Università di Foggia, 2018. http://hdl.handle.net/11369/369201.
Texto completoScientific background: Probiotics in foods could lead to significant changes in food flavor and rheology, due to their active metabolism. A possible way to overcome this problem is the attenuation of probiotics through a physical or chemical method. Some authors studied homogenization as a way to attenuate/modulate the metabolism of starter cultures in dairy products (Lanciotti et al., 2004, 2006, 2007); in this project I have used a new emerging technology, the ultrasounds. (i) A screening of the ultrasound (US) (power/duration) on different probiotic microorganisms was perfomed, and were studied the probiotic and technological characteristics after US-exposure. (ii) The effects of US on the release of intracellular components, was investigate. (iii) The interaction of attenuated strains with gut microbiota was evaluated, using in vitro batch culture fermentation. Open questions: (i) Few data are available on effects of US on probiotics and technological characteristics of probiotic strains; (ii) few data are available on the effect of attenuation with ultrasound on the sub-lethal injury; (iii) no data are available on the interaction of attenuated strains with gut microbiota. Aims: (i) Choice of the best combination of ultrasound to avoid post-acidification without affecting the viability of the strains, and study of the probiotic and technological characteristics to evaluate if attenuation could change them; (ii) study the release of intracellular components (nucleic acids and proteins) after the application of ultrasound; and (iii) evaluation the effects of attenuated strains on gut microbiota. Planning of the research: In the first part three different genera of probiotics were used: Lactobacillus, Bifidobacterium, Propionibacterium. The strains were treated with ultrasound and studied for technological and probiotic characteristics. In the second part, the effects of US on the release of intracellular components, was investigated. The strains were studied after physical treatment to assess the release of intra-cellular constituents (nucleic acids, proteins) and injury of the membrane. In the last part, the interaction of attenuated strains with gut microbiota, was studied. This study was carried out at University of Roehampton (UK). Materials and Methods: (i) Technological traits: acidification in lab medium, growth at different temperatures, pHs and salt content; probiotic traits: antibiotic-resistance, survival at pH 2.5 and in the presence of 0.3% bile salt, hydrophobicity, and biofilm formation. (ii) Injury characterization was evaluated by leakage of UV-absorbing substances. (iii) The interaction of attenuated strains with gut microbiota was evaluated, using in vitro batch culture fermentation. Results: (i) The best combinations to avoid post-acidification were the following: power, 60%; time, 6 min; pulse, 2 s for Lactobacillus and Bifidobacterium, 40%, 8 min P. jensenii; 60%, 4 min P. freudenreichii subsp. freudenreichii. US did not affect viability at 45 °C or at pH 9, but it determined a decrease of microbial growth to pH 4 (lactobacilli and bifidobacteria). However, the US did not affect the GI of propionibacteria. The effect of attenuation could be enhanced by the storage under refrigeration. US-treatment did not affect most of the technological traits, but generally caused an increase of susceptibility to some antibiotics. Concerning probiotic traits, US caused an increase of hydrophobicity for L. reuteri and P. freudenreichii spp. freudenreichii, after US-exposure. These results were confirmed with adhesion to Caco-2 cells for L. reuteri. US-attenuated L. reuteri experienced a significant increase of hydrophobicity (from 3 to 25%) and a higher adhesion to Caco-2 cells. Moreover, US improved the stability of the biofilm over the time, and this result confirmed the data obtained with hydrophobicity. (ii) The release of nucleic acids and proteins was found, highlighting that cell membrane could be another target physical treatments. (iii) Concerning the effects of US on gut microbiota, the ultrasound didn’t affect the gut microbiota, but in some cases, it could have a positive effect. Significance and Impact of PhD research: A main drawback of probiotics in foods can relate to their active metabolism, some strains of lactic acid bacteria continue to produce lactic acid and cause post-acidification (the decrease of pH within the storage). Therefore, it is important to control their metabolism. A possible way to control the metabolism of probiotic in foods is the attenuation through physical or chemical methods. One of the emerging technologies is ultrasound (US). This approach was used to avoid post-acidification in a commercial rice drink (Bevilacqua et al., 2016). The present PhD thesis contributed to evaluate the effects of attenuation with ultrasound, on some technological and probiotic strains, testing three different genera of probiotic strains. Moreover, this PhD project has investigate the changes that may affect probiotic strains after attenuation; the release of proteins, nucleic acids. Finally, the novelty of this PhD thesis was the study of the effects of attenuated strains on gut microbiota. Future trends: A future perspective could be a focus on the use of US to improve or modulate the adhesion of probiotic strains, considering the increase of hydrophobicity and the higher adhesion to Caco-2-cells. It is important to investigate the effects of other attenuated strains on gut microbiota by modulating the variables of the treatment. Key words: Hydrophobicity, acidification, growth, attenuation, gut-microbiota, proteins, nucleic acid, ultrasound, attenuated microorganisms, probiotics, lactic acid bacteria.
Lo, Raquel. "Cystine-mediated oxidative defence in Lactobacillus reuteri BR11". Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/39608/1/Raquel_Lo_Thesis.pdf.
Texto completoPereira, Adriano José. "Gradientes de oxigênio, glicose, dióxido de carbono e lactato em diferentes compartimentos vasculares". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/5/5152/tde-27102011-160323/.
Texto completoINTRODUCTION: Despite of the widespread use of the central venous oxygen saturation measurement as a therapeutic goal in critically ill patients, absolute differences between this measurement and the mixed venous oxygen exist. Causes of these differences, as well the behavior of these gradients in critical illness, are not completely understood. Considering current therapeutic interventions aimed to reverse tissue oxygenation impairment are mediated by increases in cardiac output; the particular scenario in which the heart is not physiologically able to further increase oxygen extraction and the absence of tools to monitoring the myocardium impact of those interventions, the present study, facing the theoretical possibility of the coronary sinus effluent participation in those central to mixed venous differences, has analyzed the oxygen saturation (SO2), carbon dioxide partial pressure (PCO2), lactate and glucose concentrations behaviors over time, in different models of tissue hypoxia and in different vascular sites. Emphasis on the myocardial energetic metabolism and its impact over central to mixed venous gradients was placed. METHODS: 37 pigs, males, weighting about 35 Kg, sedated and mechanically ventilated, were studied after induction of four different hypoxic injury models (sepsis, and anemic, stagnant, hypoxic hypoxia), eight for group and five controls. In addition to hemodynamic and oxygen variables, SO2, PCO2, lactate and glucose were measured in different phases, in 9 distinct vascular sites, including coronary sinus (femoral artery, inferior and superior vena cava, right atria, right ventricle, pulmonary artery, right suprahepatic vein and portal vein). MAIN RESULTS: Concentrations of O2, lactate and glucose in the coronary sinus effluent presented distinctive patterns among groups: shift from lactate to glucose consumption in hypoxic hypoxia and anemic hypoxia groups, increase in both glucose and lactate consumption in sepsis and absence of clear trend in stagnant hypoxia group. PCO2 gradients from systemic artery to coronary sinus presented late enlargement trend in all groups. In the regional gradients analysis comparisons, coronary sinus presented the lowest SO2, the lowest lactate concentrations, the highest PCO2 levels, and these patterns changed over time. Similar evolution trends were observed between central to mixed venous O2, PCO2, lactate and glucose gradients and the same parameters measured in coronary sinus. CONCLUSIONS: Different concentrations of O2, PCO2, lactate and glucose in coronary sinus are related to the type of hypoxic injury and not only to energetic substrate availability. Severity-related patterns, common to all groups in late phases, were identified: any reduction of coronary SO2, shift to glucose consumption, net lactate myocardial production and equality or inferiority of PCO2 levels related to other vascular compartments (independently of trend). Trends in transmyocardial PCO2 gradients followed cardiac output ones and, certainly, should mirror coronary blood flow. Regional gradients analysis showed suitable to explore specific regional metabolic settings, as in the described example of liver metabolism, in which production of glucose were maintained in all phases by this organ in hypoxic hypoxia groups, differently from the impaired production described in literature for sepsis. At last, data from sepsis group have showed: a) as to the previously known central to mixed venous SO2 and lactate gradients, PCO2 and glucose gradients also exist; b) coronary sinus has participated significantly in formation of central to mixed venous lactate, PCO2 and glucose gradients
Gadová, Martina. "Biotechnologická produkce vybraných metabolitů na syrovátkovém substrátu". Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2017. http://www.nusl.cz/ntk/nusl-318128.
Texto completoStuart, Mark R. "Influence of Carbohydrate Starvation on the Culturability and Amino Acid Utilization of Lactococcus lactis". DigitalCommons@USU, 1999. https://digitalcommons.usu.edu/etd/5461.
Texto completoRuhlman, Tracey. "Determinants of Chloroplast Gene Expression and Applications of Chloroplast Transformation in Lactuca Sativa and Nicotiana Tabacum". Doctoral diss., University of Central Florida, 2009. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2854.
Texto completoPh.D.
Department of Biomolecular Science
Other
Biomedical Sciences PhD
Lange-Starke, Anett. "Wirkung von Starter- und Schutzkulturen sowie ihrer Metabolite auf die Infektiosität von murinem Norovirus S 99 und Influenzavirus H1N1 in kurzgereiften Rohwürsten". Doctoral thesis, Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-154864.
Texto completoKassem, Milad. "Investigation of storage polysaccharide metabolism in lactic acid bacteria". Doctoral thesis, 2011. http://hdl.handle.net/11858/00-1735-0000-0006-AE30-5.
Texto completoCullen, Andra Jane. "The role of soluble carbohydrates in lactic acid production". 1985. http://hdl.handle.net/2097/27426.
Texto completoLI, LIN-AI y 李林璦. "The Effects of Skeletal Muscle Energy Metabolism with Long Term Oral Administration Lactic Acid Bacteria in SAMP-8 Mice". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/f6338u.
Texto completo實踐大學
食品營養與保健生技學系碩士班
105
With increasing age, skeletal muscle mass is lost, and this aging atrophy is accompanied by a reduction in muscle strength. Probiotics have known a significant health benefit to the host. Therefore, this study was to investigate the effect of Lactic acid bacteria (LAB) on skeletal muscle energy metabolism following the long-term supplementation LAB for 12 weeks in senescence-accelerated mouse P8 (SAMP8). Sixteen-week-old; male and female; SAMP8 mice were randomly delivered into 4 groups (male = 5, female = 9), including comparison group (Non-Aging) and oral administration with water (Aging), LAB at 108 CFU/day (LAB 1X), and LPF at 109 CFU/day (LAB 10X) for 12 weeks. We evaluated body weight, food intake, body composition, open field test and muscle strength test. After sacrifice, muscle, liver and blood were collected for analysis. The result showed that LAB supplementation did not change the body weight, food intake and nutrition status, but it appeared to preserve muscle mass, motility and muscle strength function. LAB supplementation increased mitochondrial biogenesis genes expression and improve mitochondrial oxygen consumption rate. Moreover, decreased inflammatory factor in serum and protein carbonyl in muscle samples, but it was unable to significantly increased glycogen levels. In conclusion, we showed that LAB maintained the expression of mitochondrial-related transcription factors and oxidative stress in SAMP8 mice. Our results indicate that LAB is a promising candidate for relief from sarcopenia.
Cruz, Fernando João Pereira da. "Genome-Scale Metabolic Network Reconstruction of the dairy bacterium Streptococcus thermophilus". Master's thesis, 2017. http://hdl.handle.net/1822/56116.
Texto completoThe dairy food industry is constantly changing as novel biotechnological techniques improve the manufacturing process of dairy products. Widely used over the years in the yogurt and cheese manufacturing, Streptococcus thermophilus is now considered as an extremely valuable lactic acid bacterium for the annual market of the dairy industry. A specific, but of easy-access knowledge regarding the thermophilic bacteria metabolism would be a plus for the continuous growth of such industry. In this work, we present the Genome-Scale Metabolic (GSM) model for the LMD- 9 strain of S. thermophilus together with the detailed description of the species metabolic capabilities at the cellular level. The reconstruction of the genome-scale metabolic model, was performed using Metabolic Models Reconstruction Using Genome-Scale Information (merlin) together with COBRApy tool and OptFlux platform. S. thermophilus LMD-9 genome was functionally annotated and the encoded metabolic information was afterwards used to assemble a draft network. After extensive manual curation, the metabolic network was converted to a comprehensive metabolic model. The assembled GSM model was then validated against experimental data. The metabolism of this important stater for the dairy industry has been accessed in detail through the reconstruction. The organism possesses a simple machinery for central carbon metabolism and shows a narrow spectrum of carbohydrate utilization. The genome-scale metabolic model additionally suggests the existence of several pyruvate dissipating pathways which end in the synthesis of various compounds of interest. In silico simulations demonstrated the production of lactate and residual amounts of formate, acetolactate and acetaldehyde. Regarding the amino acid metabolism, the organism possesses complete pathways for the biosynthesis of all amino acids, except for lysine, methionine and cysteine. Furthermore, the GSM model can be used to simulate other relevant features of the S. thermophilus metabolism, such as the aroma compounds and Exopolysaccharides (EPS) synthesis, oxygen tolerance, absence of complete citrate cycle and pentose phosphate pathway, urea metabolism or amino acid catabolism.
A indústria dos lacticínios encontra-se em constante mudança devido ao aparecimento de novas técnicas biotecnológicas que permitem o melhoramento da produção dos laticínios. Amplamente utilizado ao longo dos anos na produção de iogurte e queijo, Streptococcus thermophilus é agora considerado extremamente valioso para o mercado anual desta indústria. Portanto, conhecimento especifico, mas facilmente acessível e compreensivo sobre o metabolismo da bactéria seria uma vantagem para o crescimento continuo desta industria. Nesta tese, apresentamos o modelo metabólico à escala genómica para a estirpe LMD-9 de S. thermophilus, juntamente com um estudo aprofundado das suas capacidades metabólicas. Para obter a reconstrução do modelo metabólico à escala genómica, foi usada principalmente a ferramenta merlin com o apoio da ferramenta COBRApy e a plataforma OptFlux. O genoma de S. thermophilus LMD-9 foi anotado e as informações metabólicas codificadas foram usadas para construir uma rede rascunho. Após curação manual, a rede metabólica foi convertida num modelo metabólico à escala genómica. Posteriormente, o modelo de S. thermophilus foi validado contra dados experimentais. O metabolismo desta bactéria acido láctica foi estudado em detalhe através da reconstrução. O organism dispõe de um metabolismo de carbono muito simples e um espectro de utlização de hidratos de carbono bastante reduzido. Além disso, o modelo desenvolvido sugere a existência de várias vias metabólicas que se iniciam no piruvato e terminam na síntese de vários compostos de interesse, embora as simulações in silico tenham demonstrado apenas a produção de lactato e quantidades residuais de formato, acetolactato e acetaldeído. No que diz respeito ao metabolismo dos aminoácidos, o organismo possui as vias completas para a biossíntese de todos aminoácidos, à exceção da lisina, metionina e cisteína. O modelo pode ser usado para simular outras características relevantes de S. thermophilus, tais como a síntese de EPS e compostos aromáticos, tolerância ao oxigénio, ausência de um ciclo completo do ácido cítrico ou da via das pentoses fosfato, metabolismo da ureia ou catabolismo de aminoácidos.
Elfahri, Khaled. "Release of bioactive peptides from milk proteins by lactobacillus species". Thesis, 2012. https://vuir.vu.edu.au/21473/.
Texto completoBayigga, Lois. "Innate immune responses of vaginal epithelium and activity of monoclonal antibody-based microbicide in the presence of lactic acid, a lactobacillus metabolite". Thesis, 2016. https://hdl.handle.net/2144/16811.
Texto completoPucel, Nicholas W. "Improvement of Functional Bioactivity in Pear:Blackberry Synergies with Lactic Acid Fermentation for Type 2 Diabetes and Hypertension Management". 2013. https://scholarworks.umass.edu/theses/1149.
Texto completoCAMPONESCHI, ILARIA. "The role of the hypoxic transcription factor gene MGA2 in Kluyveromyces lactis fatty acids metabolism and cell fitness". Doctoral thesis, 2021. http://hdl.handle.net/11573/1554155.
Texto completoThe yeast Kluyveromyces lactis has been widely used in both industrial applications and basic research. We previously demonstrated that deletion of the KlMGA2, coding for a hypoxic mediator in K. lactis, generated a viable strain, although suffering of several deficiencies. We also showed that glucose signaling and glucose catabolism were involved in KlMga2 regulation. In this work, we showed that, in addition to these defects, the deletion of KlMGA2 also caused increased resistance to oxidative stress and extremely extended lifespan. These phenotypes are associated with increased expression levels of catalase and superoxide dismutase genes. We propose that KlMga2 might act as a direct mediator not only of hypoxic response, but also of oxidative stress response/adaptation, thus revealing connections between hypoxia, glucose signaling, fatty acid biosynthesis and ROS metabolism. Secondly, in this work we wanted to investigate the possible light response in this yeast. In unicellular organisms like yeasts, that cannot utilize specialized tissue for protection against environmental challenges, the presence of cellular mechanisms to respond and adapt to stress conditions is fundamental. Saccharomyces cerevisiae has been reported to respond to light by increasing hydrogen peroxide (H2O2) levels. Therefore, it could be interesting to study the possible role of oxidative stress mediator KlMga2, already studied in our laboratory, in the light response of yeast.