Literatura académica sobre el tema "Kulakov, Mikhail"

Mašīnbūves speciālista, Rīgas Politehniskā institūta (RPI) mācībspēka (1897–1918), profesora (1904) Mihaila Berlova (Михаил Берлов; 1867–1935) pedagoģiskā un zinātniskā darbība saistīta ar Rīgu 19. gadsimta beigās un 20. gadsimta pirmajā pusē, kā arī ar Krievijas pilsētu Ivanovovozņesensku (patlaban – Ivanova). M. Berlovs bija pirmais Ivanovovozņesenskas Politehniskā institūta (IPI) rektors (1918–1921), kurš 1921. gadā atgriezās Rīgā, ieguva Latvijas pavalstniecību un strādāja Nikolaja Okolo-Kulaka Krievu tehnikumā. Pētījumā atklāts arī pedagoga un zinātnieka ieguldījums mācību grāmatu sastādīšanā, kas tika izmantotas studijās Krievijas impērijā, tostarp tagadējās Latvijas teritorijā 20. gadsimta pirmajā pusē un Latvijas Republikas pastāvēšanas pirmajos gados. The pedagogical and scientific activity of a mechanical engineer, a faculty member (1897–1918) of Riga Polytechnic Institute (RPI), Professor (1904) Mikhail Berlov (Михаил Берлов; 1867–1935) at the end of the 19th century and in the first half of the 20th century was related to Riga, as well as the Russian city of Ivanovo- Voznesensk (at present – Ivanovo). M. Berlov was the first Rector of the Ivanovo- Voznesensk Polytechnic Institute (IVPI; 1918–1921), who returned to Riga in 1921, obtained Latvian citizenship, and worked at the Russian Technical School of Nikolai Okolo-Kulak. The research also revealed the contribution of the pedagogue and scientist to the compilation of textbooks that were used in the Russian Empire, including in the territory of present-day Latvia in the first half of the 20th century and in the first years of the Republic of Latvia.

The pedagogical and scientific activity of a mechanical engineer, a faculty member (1897–1918) of Riga Polytechnic Institute (RPI), Professor (1904) Mikhail Berlov (Михаил Берлов; 1867–1935) at the end of the 19th century and in the first half of the 20th century was related to Riga, as well as the Russian city of Ivanovo-Voznesensk (at present – Ivanovo). M. Berlov was the first Rector of the Ivanovo-Voznesensk Polytechnic Institute (IVPI; 1918–1921), who returned to Riga in 1921, obtained Latvian citizenship, and worked at the Russian Technical School of Nikolai Okolo-Kulak. The research also revealed the contribution of the pedagogue and scientist to the compilation of textbooks that were used in the Russian Empire, including in the territory of present-day Latvia in the first half of the 20th century and in the first years of the Republic of Latvia.

Creation of Soviet leader official biography assumed using of class patterns. This formed an exclusion zone connected with stigmatization of a family. The soviet leader – “faithful son of the party” – was a man out of nowhere, a production of social industry. However, social experience linked with the family could determine the character’s attitude to major landmarks of the epoch. But some information about the true origin didn’t disappear. It was accumulated to be used as a tool of social control within the framework of repressive policies. The stigmatization of a family as an important part of social rise during the soviet period is studied basing on the biography materials of Army General A. V. Khrulev. The official biography contained mix of real and fictional information, which were constructed following ideological patterns. The true status of Khrulev’s family was reconstructed on the basis of non-published archival materials. The status of Khrulev’s family caused problems for its members who occupied high positions in soviet hierarchy: they were accused of using hired labor (being kulaks) in 1930; of using position by some family members (Mikhail was a deputy chairman of the Murmansk Executive Committee, Andrei – a high ranking military commander). In 1938 Mikhail Khrulev was convicted under article 58 of Criminal Code of RSFSR, and that transformed the status of all relatives: they became “family members of traitor to Homeland”. In this research, the influence of these facts is studied, forming the attitude of Army General A. V. Khrulev to repressive policy of the Soviet state at 1930s – early 1950s, and, namely, to those persons who were responsible for carrying out repressive policy in practice.

Abstract Introduction: Detection of mutation and methylation patterns in liquid biopsies has emerged as a promising approach for screening early-stage cancers. We have developed a targeted next-generation sequencing (NGS)-based assay “OptiSeqTM Colorectal Cancer Detection Panel”, a combined platform for assessing mutation and methylation in both low-quantity (plasma cfDNA) and low-quality formalin-fixed paraffin-embedded (FFPE) tissue for colorectal cancer (CRC) detection. Methods: The OptiSeqTM Colorectal Cancer Detection Panel targets 442 SNVs and 434 CpG sites to detect circulating tumor DNA (ctDNA) in early-stage patients. With this panel, we profiled 6 early-stage and 6 metastatic CRC samples (matching cfDNA and FFPE) along with 2 primary colon specimens with adjacent normal tissue DNA. We also profiled 2 matching cfDNA and FFPE samples from precancerous adenomas (advanced adenomas (AA)) and 6 AA FFPE tissue DNA. In addition, we characterized the mutation and methylation pattern in 10 CRC fresh-frozen tissue DNA samples. For healthy individuals, we profiled 12 normal colon FFPE samples and 10 plasma cfDNA. The clinical performance for the CRC detection panel was calculated using AA, CRC, and cancer-free individuals. Results: For CRC early and metastatic matching cfDNA and FFPE samples, clinical samples testing showed that the CRC detection panel had a sensitivity of 100% (95% CI: 69.8-100.0) and specificity of 100% (95% CI: 81.5-100). For precancerous AA FFPE tissues, combined DNA mutation and methylation increased the CRC detection sensitivity to 100% (95% CI: 51.6-100.0) and specificity to 100% (95% CI: 61.8-100). More clinical samples testing is underway. Conclusion: We have developed the targeted NGS-based panel to detect clinically relevant mutation and methylation patterns and demonstrate its potential using the combined genetic and epigenetic markers making it a highly sensitive assay for early CRC diagnosis. Citation Format: Pushpinder Kaur Bains, Mikhail Kulak, Zhen Cui, Alisha Babu, Stella Tran, Aiguo Zhang, Michael Sha. Circulating mutated and methylated tumor DNA detection for colorectal cancer diagnosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 5021.

Abstract Introduction: The ability to identify epigenetic signatures, together with genetic alterations, has expanded the translational utility of genomic sequencing in clinical settings. Epigenetic changes may appear at very early stages before diagnosis. Thus, they could be used as epigenetic biomarkers for early detection or risk of cancer development. Circulating cell-free DNA (cfDNA) profiling has emerged as a promising biomarker for cancer diagnosis and prognosis. Herein, we developed targeted next-generation sequencing (NGS) based assays for assessing multiple DNA methylated and mutated regions in both low-quantity (plasma cfDNA) and low-quality formalin-fixed paraffin-embedded (FFPE) tissue for colorectal cancer (CRC) detection. Methods: For the OptiSeqTM CRC methylation panel, we assessed the methylation status of CpG dinucleotides in 42 genes. With this panel, we profiled 6 CRC samples (matching cfDNA and FFPE), 2 primary colon tumors with adjacent normal, and 6 advanced adenomas (AA) FFPE tissue DNA. For healthy individuals, we profiled 12 normal colon FFPE samples and 31 plasma cfDNA (26 matching buffy coat DNA samples). For the OptiSeqTM CRC mutation panel, the mutation status was assessed in 43 genes. Here, we profiled 6 CRC samples (matching cfDNA and FFPE), 19 CRC plasma cfDNA, 4 AA FFPE and 42 healthy individuals’ plasma cfDNA (26 matching buffy coat DNA samples). The analytical and clinical performance for both panels (separately and combined) were calculated using advanced adenoma (AA), CRC, and cancer-free individuals. Results: For CRC cfDNA samples, clinical samples testing showed that the methylation panel had a sensitivity of 50% (95% CI: 13.9-86.0) and specificity of 100% (95% CI: 86.2-100) while the mutation panel yielded the sensitivity 88% (95% CI: 67.7-96.8) and specificity 100% (95% CI: 89.5-100). Combining the mutation panel with the methylation panel, the sensitivity increased to 100% (95% CI: 51.6-100) and specificity to 100% (95% CI: 65.1-93.3). For AA FFPE samples, the methylation panel analysis yielded a sensitivity of 100% (95% CI: 39.5-100.0) and a specificity of 100% (95% CI: 67.8-100). Conclusion: We have developed and validated the targeted NGS-based panels to detect clinically relevant mutation and methylation patterns and demonstrate its potential as a highly sensitive assay for early CRC diagnosis. Citation Format: Pushpinder Bains, Mikhail Kulak, Zhen Cui, Alisha Babu, Stella Tran, Bongyong Lee, Aiguo Zhang, Michael Sha. Detection of tumor-associated methylation and mutation signatures for early colorectal cancer diagnosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 2302.

Abstract The Small Ubiquitin-like Modifier (SUMO) pathway is a major post-translational regulatory mechanism involved in a variety of cellular functions including the DNA damage response, transcriptional regulation, and carcinogenesis. Recent discoveries by our group demonstrated that inhibiting the SUMO pathway can repress the growth of breast cancer xenografts and Neu-driven mammary oncogenesis in a genetically engineered mouse model with conditional knockout (CKO) of the SUMOylation E2 conjugating enzyme, Ube2i. However, despite the consistency of the findings, there remains considerable debate concerning the mechanisms through which SUMO inhibition represses tumorigenesis. Our downstream analysis suggested ETS Translocation Variant 1, encoded by ETV1 gene, acts as a SUMO-sensitive transcription factor that drives mammary oncogenesis and cancer growth. To examine oncogenic properties of SUMO-conjugated ETV1 in breast cancer, we cloned HA-tagged WT Etv1, SUMO-resistant Etv15xKR, or SUMO mimetic SUMO-1-Etv15xKR into a lentiviral vector with a GFP cassette. Proliferation, colony formation in soft agar, and in vivo xenograft assays were evaluated in the Ube2iF/F cell line derived from a tumor in a MMTV-c-neu/Ube2ifl/fl animal. We did ChIP-seq in Ube2iF/F cells using SUMO-1 and Etv1 antibodies to examine the effects of SUMO conjugation on Etv1 genomic occupancy. RNA-seq using siEtv1 or AdCre was also performed to compare changes in gene expression with SUMO-conjugated Etv1 genomic occupancy. Inhibition of SUMOylation via AdCre caused a significant reduction in proliferation and colony formation in vitro and completely block the formation of flank xenografts in vivo. Interestingly, expressing a SUMO-resistant ETV15xKR rescued in vitro growth effects, whereas a SUMO mimetic SUMO-1-ETV15xKR had no effect on restoring proliferation. On the contrary, in vivo xenograft assays demonstrated that the Etv15xKR-SUMO1 efficiently induced tumor formation despite the loss of Ube2i while the sumo-resistant Etv15xKR was unable to rescue tumorigenesis. Bulk RNA-seq and ChIP seq performed demonstrated different patterns of gene regulation between sumoylated and unsumoylated Etv1. Gene set enrichment analysis (GSEA) showed genes associated with cancer stemness were significantly enriched in cells expressing Etv15xKR-SUMO1. Collectively, these observations support Etv1 acting as a SUMO-sensitive transcription factor that drives a stem cell phenotype necessary for mammary oncogenesis and progression. Citation Format: Zhijie Li, Kelsey E. Koch, Dakota T. Thompson, Dana M. Van der Heide, Jeremy Chang, Christopher M. Franke, Mohammed O. Suraju, Anna C. Beck, Allison W. Lorenzen, Jeffrey R. White, Mikhail V. Kulak, David K. Meyerholz, Colin Kenny, Ronald J. Weigel. Understanding the role of sumoylated Etv1 in mammary oncogenesis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 6605.

В статье рассматриваются образы сказителей из с. Походск Нижнеколымского края XIX в. Михаила Соковикова и Митрофана Кривогорницына, певцов былин и исторических песен. Приводятся произведения фольклора, которые были записаны от Михаила Соковикова, произведенные В. Г. Богоразом. Также имеется несколько произведений, которые, возможно, записаны от этого исполнителя или от сказителя Митрофана Кривогорницына. Для изучения авторства исполнителя в записях Д. И. Меликова проводится сравнительно-описательный анализ имеющихся вариантов, сравнение записей проводится на уровне мотивов, индивидуального стиля. Сравниваются некоторые особенности текстов былин «Добрыня и змеинище Горынище», «Алеша Попович и Тугарин», «Илюша и Идолище», «Дюк Степанович», «Потоп Михайлович», «О Мишеньке Даниловиче», «Иван Кулаков», исполненных М. Ф. Соковиковым. Рассматривается былина «Илья Муромец на Соколе-корабле», записанная от М. Кривогорницына.Также изучаются исторические песни «О Скопине», «Заявился в камен город детинушка», «Ах, талан, ты мой таланчик» и приводится баллада «Ключник», исполненные неизвестным сказителем в записи Меликова.В ходе исследования выясняется, что стилю Михаила Соковикова свойственны определенные былинные формулы. Так, уточняются формула «входа в гридню», эпизод обувания.На основе сравнения вариантов песни о графе Чернышове можно предположить, что исполнитель, чье творчество зафиксировал Д. И. Меликов, это Митрофан Кривогорницын.Предстоит и дальше изучать вопросы, связанные с уточнением авторства некоторых произведений фольклора, записанных Д. И. Меликовым. М. Ф. Соковиков имел детей (Иван Соковиков – Куларенок, Арина Четверикова), чье творчество также следует изучать. In article reviews the images of narrators from the village of Pokhodsk, Nizhnekolymsky land, of the XIX century – Mikhail Sokovikov and Mitrofan Krivogornitsyn, singers of bylinas and historical songs. There are works of folklore which were recorded by V. G. Bogoraz from Sokovikov. Also, there are some works which, perhaps, are recorded from this performer or from the narrator Mitrofan Krivogornitsyn. For studying the authorship of the performer in D. I. Melikov`s records, the comparative and descriptive analysis of the available texts is carried out, as well as comparison of records at the level of motives and individual style. Some features of texts of the bylinas "Dobrynya and Zmeinishche Gorynishche", "Alyosha Popovic and Tugarin", "Ilyusha and Idolishche", "Duke Stepanovich", "Potop Mikhaylovich", "About Mishenke Danilovich", "Ivan Kulakov" performed by M. F. Sokovikov are compared. The bylina "Ilya Muromets on the Falcon Ship" which is recorded from M. Krivogornitsyn is considered.We also studied historical songs "About Skopin", "Detinushka Entering a Stone Town", "Ah, Talan, My Little Talan" are and the ballad "Key Keeper", performed by an unknown narrator in Melikov's record.During the research, it becomes clear that certain epic formulas are peculiar to Sokovikov's style. The formulas of "the entrance in gridnya" and the process of footwear putting on are to be finalized.Basing on the comparison of the versions of the song about the Count Chernyshov, it is possible to assume that the performer, whose creativity D. I. Melikov recorded, was Mitrofan Krivogornitsyn.It is necessary to study further the questions connected with identification of authorship of some works of folklore which are recorded by D. I. Melikov. M. F. Sokovikov had children (Ivan Sokovikov – Kularenok, Arina Chetverikova), whose creativity also should be studied.