Literatura académica sobre el tema "ISCAT"

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Artículos de revistas sobre el tema "ISCAT"

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Davis, D. D., F. Eisele, G. Chen, J. Crawford, G. Huey, D. Tanner, D. Slusher et al. "An overview of ISCAT 2000". Atmospheric Environment 38, n.º 32 (octubre de 2004): 5363–73. http://dx.doi.org/10.1016/j.atmosenv.2004.05.037.

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Strack, Rita. "iSCAT gets a signal boost". Nature Methods 18, n.º 5 (mayo de 2021): 447. http://dx.doi.org/10.1038/s41592-021-01160-0.

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Shi, Zhonghong, Jiufeng Huang, Xi Huang, Yangwei Huang, Lijun Wu y Qiang Li. "Resonant scattering enhanced interferometric scattering microscopy". Nanoscale 12, n.º 14 (2020): 7969–75. http://dx.doi.org/10.1039/c9nr10391k.

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Young, Gavin y Philipp Kukura. "Interferometric Scattering Microscopy". Annual Review of Physical Chemistry 70, n.º 1 (14 de junio de 2019): 301–22. http://dx.doi.org/10.1146/annurev-physchem-050317-021247.

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Interferometric scattering microscopy (iSCAT) is an extremely sensitive imaging method based on the efficient detection of light scattered by nanoscopic objects. The ability to, at least in principle, maintain high imaging contrast independent of the exposure time or the scattering cross section of the object allows for unique applications in single-particle tracking, label-free imaging of nanoscopic (dis)assembly, and quantitative single-molecule characterization. We illustrate these capabilities in areas as diverse as mechanistic studies of motor protein function, viral capsid assembly, and single-molecule mass measurement in solution. We anticipate that iSCAT will become a widely used approach to unravel previously hidden details of biomolecular dynamics and interactions.
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Blosser, Matthew C., Helena L. E. Coker y Mark I. Wallace. "Nanoscopic Lipid Domains Characterized by iSCAT Microscopy". Biophysical Journal 112, n.º 3 (febrero de 2017): 385a. http://dx.doi.org/10.1016/j.bpj.2016.11.2092.

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Blosser, Matthew C., Helena L. E. Coker y Mark I. Wallace. "iSCAT Microscopy of Phase Separated Lipid Membranes". Biophysical Journal 114, n.º 3 (febrero de 2018): 449a. http://dx.doi.org/10.1016/j.bpj.2017.11.2482.

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Walter, Vivien y Mark I. Wallace. "Real-Time Point Spread Function Engineering for iSCAT". Biophysical Journal 118, n.º 3 (febrero de 2020): 464a. http://dx.doi.org/10.1016/j.bpj.2019.11.2577.

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de Wit, Gabrielle, John S. H. Danial, Philipp Kukura y Mark I. Wallace. "Real-Time Imaging of Nanoscopic Lipid Domains using iSCAT". Biophysical Journal 108, n.º 2 (enero de 2015): 17a—18a. http://dx.doi.org/10.1016/j.bpj.2014.11.121.

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Lu, Hui-Meng, Jing-Di Li, Yu-Dan Zhang, Xiao-Li Lu, Chang Xu, Yuan Huang y Michael Gribskov. "The Evolution History of Fe–S Cluster A-Type Assembly Protein Reveals Multiple Gene Duplication Events and Essential Protein Motifs". Genome Biology and Evolution 12, n.º 3 (27 de febrero de 2020): 160–73. http://dx.doi.org/10.1093/gbe/evaa038.

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Abstract Iron–sulfur (Fe–S) clusters play important roles in electron transfer, metabolic and biosynthetic reactions, and the regulation of gene expression. Understanding the biogenesis of Fe–S clusters is therefore relevant to many fields. In the complex process of Fe–S protein formation, the A-type assembly protein (ATAP) family, which consists of several subfamilies, plays an essential role in Fe–S cluster formation and transfer and is highly conserved across the tree of life. However, the taxonomic distribution, motif compositions, and the evolutionary history of the ATAP subfamilies are not well understood. To address these problems, our study investigated the taxonomic distribution of 321 species from a broad cross-section of taxa. Then, we identified common and specific motifs in multiple ATAP subfamilies to explain the functional conservation and nonredundancy of the ATAPs, and a novel, essential motif was found in Eumetazoa IscA1, which has a newly found magnetic function. Finally, we used phylogenetic analytical methods to reconstruct the evolution history of this family. Our results show that two types of ErpA proteins (nonproteobacteria-type ErpA1 and proteobacteria-type ErpA2) exist in bacteria. The ATAP family, consisting of seven subfamilies, can be further classified into two types of ATAPs. Type-I ATAPs include IscA, SufA, HesB, ErpA1, and IscA1, with an ErpA1-like gene as their last common ancestor, whereas type-II ATAPs consist of ErpA2 and IscA2, duplicated from an ErpA2-like gene. During the mitochondrial endosymbiosis, IscA became IscA1 in eukaryotes and ErpA2 became IscA2 in eukaryotes, respectively.
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Feng, Qingzhou, Allison M. Gicking y William O. Hancock. "Dynactin p150 promotes processive motility of DDB complexes by minimizing diffusional behavior of dynein". Molecular Biology of the Cell 31, n.º 8 (1 de abril de 2020): 782–92. http://dx.doi.org/10.1091/mbc.e19-09-0495.

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Dynein–dynactin–BicD2 (DDB) is highly processive, but also shows transient pausing and diffusion, which we analyzed using iSCAT microscopy. Blocking dynactin p150 results in more diffusion of isolated DDB and a plus-end shift of kinesin-1–DDB complexes. Thus, we conclude that p150 is an allosteric activator of dynein in the DDB complex.
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Tesis sobre el tema "ISCAT"

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Olive, Joshua A. "Investigating the Roles of the Iron-Sulfur Proteins Monothiol Glutaredoxin 5, ISCA1, and ISCA2". The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1503305827105994.

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Beilschmidt, Lena Kristina. "Evidences for the non-redundant function of A-type proteins ISCA1 and ISCA2 in iron-sulfur cluster biogenesis". Thesis, Strasbourg, 2014. http://www.theses.fr/2014STRAJ031/document.

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Les centres fer-soufre (Fe-S) sont des cofacteurs protéiques essentiels qui participent à un nombre important de fonctions cellulaires allant du métabolisme de l’ADN à la respiration mitochondriale. L’assemblage des centres Fe-S et leur insertion dans des protéines acceptrices requièrent l’activité d’une machinerie protéique dédiée. Bien que les protéines de la biogenèse des centres Fe-S soient conservées, plusieurs aspects fonctionnels et mécanistiques restent inconnus. Notre travail de thèse a consisté à caractériser les protéines mammifères de type A, ISCA1 et ISCA2, qui sont impliquées dans la biogenèse mitochondriales des centres Fe-S. En utilisant une approche couplant l’immunoprécipitation avec une analyse protéomique par spectrométrie de masse, plusieurs interactions protéiques d’ISCA1 et ISCA2 ont pu être identifiées. En plus d’une interaction entre ISCA1 et ISCA2, nous avons ainsi montré l’existence d’interactions spécifiques à chacune de ces protéines. Une approche de knockdown dans la souris via l’injection de virus adéno-associés, a permis de montrer l’absence de redondance fonctionnelle entre ISCA1 et ISCA2 puisque seul ISCA1 se trouve être nécessaire dans la maturation d’une catégorie de protéines à centre Fe-S
Iron-sulfur clusters (Fe-S) are essential cofactors involved in different cellular processes ranging from DNA metabolism to respiration. Assembly of Fe-S clusters and their insertion into acceptor proteins is performed by dedicated protein machineries. Despite the high conservation from bacteria to man, different functional and mechanistic aspects of the Fe-S biogenesis remain elusive. In the present work, the function of the two mammalian A-type proteins ISCA1 and ISCA2 that are implicated in Fe-S biogenesis was investigated in vivo. First, an extensive analysis coupling immunoprecipitations and mass spectrometry led to the identification of a direct binding between ISCA1 and ISCA2 as well as specific protein partners of each protein. Furthermore, knockdown experiments in the mouse using adeno-associated virus provided clear evidence of the non-redundant function of ISCA1 and ISCA2, since only ISCA1 was shown to be required for a specific subset of mitochondrial Fe-S proteins
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Yeşilova, Ecehan. "Konişmentonun ispat kuvveti /". İzmir : Güncel, 2006. http://swbplus.bsz-bw.de/bsz273083295inh.pdf.

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Stümpfig, Claudia [Verfasser] y Roland [Akademischer Betreuer] Lill. "Funktionelle Defekte der humanen Proteine ISCA1, ISCA2 und IBA57 beeinträchtigen die Reifung mitochondrialer Fe/S-Proteine und führen zu mitochondrialen Erkrankungen / Claudia Stümpfig. Betreuer: Roland Lill". Marburg : Philipps-Universität Marburg, 2015. http://d-nb.info/1069375187/34.

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Ljungström, Erica. "ISAT : Interactive Scenario Analysis Tool for financial forecasting". Thesis, KTH, Skolan för informations- och kommunikationsteknik (ICT), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-177128.

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Målet för denna studie har varit att skapa en första version för ett verktyg i vilket analytiker inom finans kan skapa sina långsiktiga scenarion och väga olika rikser samt möjligheter mot varandra.Idén till ett sådant verktyg har funnits inom företaget i flera år, men de tidigare idéerna har varit för specifika för att kunna användas. Detta har främst berott på tidsbrist samt att de som utvecklat dem inte har haft tillgång till en bra utvecklingsmiljö.De enda begränsningarna för verktyget har varit 1) ”Det behöer kunna visa inverkan av manipuleringar”, 2) ”det behöer finnas såmycket funktionalitet som möjligt, utan att det finns knappar överallt” och 3) ”det få inte ädra nåot av input datan”.Eftersom dessa är abstrakta specificationer behövdes mock-ups, observationer och användbarhetstest för att kunna skapa ett verktyg som förenklar de mest använda manipulationerna. Verktyget måste också låta användaren enkelt ticka i och ur sina manipulationer så att dessa inte behöver göras om varje gång användaren vill testa ett nytt utfall.Observationerna och testerna har visat att användarna jobbar på olika sätt, och därför behövde verktyget vara flexibelt. Detta innebar också att det behövdes både generella samt specifika manipulationer. De visade också att verktyget behövde delas in i två delar, en för att skapa rapporter och en för att visa rapporten. Detta då rapporteringsprocessen ej får ändras.Fokus för denna studie har varit MDI, Människa-datorinteraktion. Detta innebär att den färdiga produkten bör vara intuitiv och väldigt enkel att lära sig att använda för användarna. Detta kan vara svårt då användarna arbetar på mycket olika sätt.Den färdiga produkten för denna studie har lyckats klara alla de mål som satts upp. En mock-up som gjorde användarna nyfikna på programmet, skapades i Java. Det bestämdes därmed att detta var det programmeringsspråk som skulle användas. Ett användargränssnitt som var enkel, men samtidigt hade en komplex funktionalitet lades till.Detta gjorde att användarna frågade sig själva ”Kan det verkligen vara såhä enkelt?” samt ”Varfö har vi inte gjort detta innan?”. Tillslut skapades en fungerande produkt som var både enkel att använda samt att den gjorde många av de enkla beräkningarna åt analytikern.Den enda del som ej blivit fullt implementerad innan slutet för denna studie är mallen för de Excel rapporter som ska skapas av verktyget. Denna del av verktyget utformades av en ekonom som vet vilka grafer som kan vara intressanta att ta med i en rapport. Nu, när verktyget genererar en rapport, genereras endast de grafer som syns i verktyget, totalen för scenariona (uppdelade i olika kategorier) samt alla de manipuleringsrader som skapats för de tre olika scenariona.
The goal with this study has been to create a first version for a tool in which financial analysts can create their long-term scenarios and weigh different risks and opportunities against each other.The idea to such a tool has been around for years within the company, but the earlier ideas were too specific to be usable. This has mainly been due to the lack of time and available tools to realize the ideas.The only restrictions for the tool have been 1) “It needs to show the impact of manipulations”, 2) “it needs as much functionality as possible without having buttons all over it” and 3) “it should not alter any of the input data”.Because these are quite abstract specifications, mock-ups, observations and usability tests have been used to create a tool that simplifies the most used manipulations and enables the user to tick in and out their manipulations so that the manipulation does not have to be redone every time the user wants to test a new outcome.The observations and tests have shown that the users work very differently from each other, and so, the tool needed to be very flexible. This meant that there needed to be both general and specific manipulations which are based on general formulas. It also showed that the tool needed to be split into two parts, one for creating and one for showing reports, because the reporting process should not be altered.The focus of this study has been HCI, Human Computer Interaction. This means that the finished product should be intuitive and also easy to learn how to operate by the users which could be difficult when the users do work in different ways. The resulting product of this study has reached all of the goals. A mock-up that got the users interested in the program was produced in Java, which decided the programming language. A GUI that was simple, yet had complex functionality was added. It made users ask themselves “Could it really be this easy?” and “Why have we not done this before?”. And, at last, a working product were produced, that was both simple to operate and at the same time did a lot of the calculations for the analyst.The only part of the product that has not been fully implemented before the endof this study is the template in which the Excel Report is supposed to be generated. This part of the tool was taken care of by an economist that knew which graphs that could be interesting to create a report of. Now, the tool generates a report with only the graphs that are shown in the tool, the totals for the scenarios (split into different categories) and all of the adjustment rows for the three scenarios.
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Tellez, Guio Leonardo. "Iscas formicidas com fipronil e sulfluramida e Apis mellifera (Hymenoptera: Apidae)". Universidade Federal de Viçosa, 2017. http://www.locus.ufv.br/handle/123456789/10059.

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Iscas atrativas com os ingredientes ativos fipronil e sulfluramida são utilizadas no controle de formigas cortadeiras e substâncias alimentícias nestas iscas podem atrair e expor abelhas a esses tóxicos, o que pode alterar o comportamento e reduzira sobrevivência desses insetos. O objetivo foi determinar a toxicidade e atração das iscas a Apis mellifera (Hymenoptera: Apidae). Operarias dessa abelha foram expostas, por ingestão de solução de açúcar 50% (xarope) com fipronil ou sulfluramida quanto por contato direto e inalação dos voláteis das iscas formicidas tendo cada unidade experimental 10 abelhas/pote. A mortalidade foi registrada após um, dois, quatro, seis, 12, 24 e 48 horas nos tratamentos e nos controles: xarope e atraentes da isca sem ativo. Cinco gramas das iscas foram disponibilizadas no campo às abelhas em pratos plásticos junto com o xarope (alimento com 30% de à açúcar) ao nível do solo. O número de abelhas que pousaram sobre as iscas, foi registrado a cada 30min em seis observações por dia e comparado com o daquelas atraídas no controle (mel à 50%). A sobrevivência de forrageiras de A. mellifera foi menor com as concentrações da isca fipronil com DL 50 oral 48 h de 54,5 ± 12,9 μg isca/abelha. Abelhas expostas por ingestão da solução com a isca formicida sulfluramida tiveram sobrevivência semelhante a do controle. A mortalidade de abelhas após 48 h de contato direto com as iscas foi >86,0%, com efeito letal dos voláteis da isca com sulfluramida (contato ou inalação), maior que o controle. O numero de abelhas forrageiras que posou sobre as iscas formicidas com fipronil e sulfluramida oscilou entre 11,0 e 0,0, menor que daquelas atraídas no controle (mel à 50%) 143,0 a 114,3. Iscas formicidas com fipronil e sulfluramida são “perigosos e pouco perigosos” respectivamente, para A. mellifera por ingestão. Tem potencial toxico por contato direto e com os voláteis da isca com sulfluramida, mas no campo não foram atraentes as abelhas.
Attractive baits with the active ingredients fipronil and sulfluramide are used in the control of leaf cutting ants and food substances in these baits can attract and expose bees to these toxins, which can alter the behavior and reduce the survival of these insects. The objective was to determine the toxicity and attraction of the baits to Apis mellifera (Hymenoptera: Apidae). Operators of this bee were exposed by ingestion of 50% sugar solution (syrup) with fipronil or sulfluramide and by direct contact and inhalation of the volatiles of the formicidal baits with each experimental unit 10 bees/pot. The mortality was registered after one, two, four, six, 12, 24 and 48 hours in the treatments and in the controls: syrup and attractive of the bait without assets. Five grams of the baits were made available in the field to the bees in plastic plates with the syrup (feed with 30% of the her sugar) at the level of the soil. The number of bees that landed on the baits was recorded every 30 minutes in six observations per day and compared to those attracted to the control (honey at 50%). The survival of forages of A. mellifera was lower with the concentrations of fipronil bait with oral LD 50 48 h of 54.5 ± 12.9 μg bait/bee. Bees exposed by ingestion of the solution with the formicidal sulfluramide bait had similar survival to that of the control. The mortality of bees after 48 hours of direct contact with the baits was >86.0%, with a lethal effect of the volatiles of the bait with sulfluramide (contact or inhalation), greater than the control. The number of forage bees that posed on the baits formulated with fipronil and sulfluramide oscillated between 11.0 and 0.0, lower than those attracted in the control (honey at 50%) 143.0 to 114.3. Baits formicidal with fipronil and sulfluramida are “dangerous and little dangerous” respectively, for A. mellifera by ingestion. It has toxic potential by direct contact and with the volatiles of the bait with sulfluramida, but in the field the bees were not attractive.
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Bastos, Maria da Piedade Rodrigues. "O Balanced Scorecard aplicado ao ensino superior: o caso do ISCAA". Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/10442.

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Mestrado em Administração e Gestão Pública
Nas últimas décadas, as organizações públicas têm sido pressionadas para que os seus serviços se tornem mais transparentes, responsáveis e próximos dos cidadãos, de modo a satisfazer as suas expectativas e necessidades prestando um melhor serviço público. A pressão para esta mudança resulta da mundialização da economia, do desenvolvimento das Tecnologias da Informação e Comunicação, da redefinição de fronteiras e da alteração da ligação entre o Estado e a Sociedade. Esta transformação tem-se traduzido na descentralização de competências, maior atenção atribuída às exigências sociais decorrentes da crise económica, crescente exigência dos cidadãos para com os governantes, desburocratização e racionalização dos serviços públicos. Neste contexto, os gestores públicos terão de enfrentar grandes desafios para transformar a Administração Pública e adequá-la a uma sociedade mais informada e exigente. O recurso a práticas, ferramentas e modelos utilizados no setor privado decorre precisamente da pretensão de melhorar substancialmente a qualidade dos seus serviços, procurando colocá-los ao nível dos melhores do setor privado. A avaliação de desempenho é uma destas práticas. Assim, este trabalho de projeto tem como objetivo conceber um modelo de BSC para aplicar no Instituto Superior de Contabilidade e Administração de Aveiro (ISCAA) de forma a sustentar a formulação, execução e avaliação da estratégia da Instituição. Inicia-se com uma revisão da literatura efetuando um enquadramento geral que apresenta os principais modelos de avaliação de desempenho, com especial destaque para o Balanced Scorecard. Neste projeto efetua-se a definição dos eixos estratégicos de atuação, perspetivas de avaliação, objetivos estratégicos e relações de causalidade entre os objetivos estratégicos associados a cada perspetiva. Conclui-se que o modelo de Balanced Scorecard concebido e descrito neste trabalho, para o ISCAA, não é um trabalho terminado, mas um ponto de partida que pretende contribuir para a definição, avaliação e controlo da estratégia da instituição.
In recent decades public organizations have been pressed so that their services become more transparent, accountable and closer to citizens, in order to meet their expectations and needs by providing better public services. The pressure for this change results from the economic globalization, the development of Information Technologies and Communication, and also from redefining boundaries and changing the links between the State and Society. This transformation has resulted in a growing decentralization, greater attention given to social needs resulting from the economic crisis, growing citizen’s demands towards government, bureaucracy and rationalization of public services. In this context, public managers will face major challenges to transform public administration and adjust it to become more informed and demanding. The adoption of practices, tools and models used in the private sector derives precisely from the claim of substantially improving the quality of the services provided. Performance evaluation is precisely one of these practices. This study project aims to design a model to be applied the BSC in the Instituto Superior de Contabilidade e Administração de Aveiro (ISCAA) in order to support the formulation, implementation and evaluation of the strategy of that Institution. It begins with a literature review by the main models for performance evaluation, with special emphasis on the Balanced Scorecard. We essentially focus on the definition of the strategic axes of performance, evaluation perspectives, strategic goals and causal relationships between the strategic objectives associated with each perspective. We conclude that the Balanced Scorecard model designed and described in this work for ISCAA is not a finished work, but a starting point that is intended to contribute to the definition, assessment and control strategy of the Institution.
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Tofolo, Viviane Cristina [UNESP]. "Dinâmica populacional, forrageamento e exposição de operárias de Ectatomma opaciventre (Hymenoptera : Formicidae) a iscas formicidas contendo sulfluramida, fipronil e clorpirifós". Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/106532.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Ectatomma opaciventre (Hymenoptera: Formicidae: Ectatomminae) apresenta características morfológicas e comportamentais plesiomórficas, como a presença de um ferrão funcional, monoginia, colônias pequenas e estratégia de forrageamento solitário. Vive tanto nos ambientes preservados quanto nos alterados pelo homem, como os agroecossistemas, estando constantemente em contato com os produtos destinados ao controle de formigas consideradas pragas, como as cortadeiras do gênero Atta. Pouco se conhece a respeito de sua biologia básica (como dieta, rotas de forrageamento, distribuição espacial dos ninhos, composição dos hidrocarbonetos cuticulares) e das conseqüências advindas de seu contato com as iscas formicidas usadas para o controle de formigas cortadeiras em pastagens. Dessa forma, foi determinado por meio do “Método do Vizinho Mais Próximo” o padrão de distribuição espacial de seus ninhos em uma área degradada de 1800m2 na cidade de Rio Claro/SP/Brasil. Como na maioria das espécies de formigas tropicais, os ninhos de E. opaciventre apresentaram distribuição espacial do tipo regular ou hiperdispersa, com densidade de 0,018 ninho/m2. A distância média encontrada para o vizinho mais próximo foi de 5,57m, variando de 1,93m a 12,96m. Em vista da dificuldade em encontrar a rainha desta espécie, foi utilizado o método da “Espectroscopia Fotoacústica no Infravermelho com Transformada de Fourier (FTIR)” para identificar os hidrocarbonetos cuticulares da única rainha encontrada e das operárias de 9 ninhos satélites. Também foi determinado o grau de similaridade entre elas. Com os resultados obtidos ficou evidenciada a condição polidômica nessa espécie devido à semelhança entre o perfil químico cuticular da rainha e das operárias de ninhos vizinhos. Além disso...
Ectatomma opaciventre belongs to the subfamily Ectatomminae, which presents morphological and behavioral characteristics typical of more basal groups, such as the presence of a functional sting, monogyny, small colonies, and individual foraging strategies while searching for food. This species lives both in preserved and modified environments, such as agroecosystems, and it is constantly in contact with products intended to control ants regarded as pests, such as leaf-cutting ants of the genus Atta. The patterns of spatial distribution of nests over a 1800m2 degraded area in the city of Rio Claro, SP, Brazil were determined using a nearest neighbor method. As in most tropical species, the nests of E. opaciventre presented a regular or hyperdispersed spatial distribution, with a density of 0.018 nests/m2. The average nearest neighbor distance was 5.57 m, ranging from 1.93 m to 12.96 m. Considering the difficulty in finding queens of this species, the Fourier Transform-Infrared Photoacoustic Spectroscopy (FTIR-PAS) was used to identify cuticular hydrocarbons of the only queen found and of workers from nine satellite nests. The degree of similarity between them was also determined. From the results, polydomy was evident in this species because of the similarity between the cuticular chemical profile of the queen and that of workers from neighboring nests. Moreover, it was observed that the colonies that were not collected disappeared one year after the removal of the queen from the study area. In another area in the city of Rio Claro, of 4,800 m2, the diet of this species was studied by identifying the prey collected during the period of highest foraging activity. Besides, the foraging routes of three nests were determined by following foragers during their excursions in the field. The maximum foraging distance was... (Complete abstract click electronic access below)
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Acevedo, Isabel Cristina Chica. "Estudos de ação de novos peptídeos antimicrobianos derivados do IsCT". reponame:Repositório Institucional da UFABC, 2016.

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Orientador: Prof. Dr. Vani Xavier de Oliveira Junior
Dissertação (mestrado) - Universidade Federal do ABC. Programa de Pós-Graduação em Ciência e Tecnologia/Química, 2016.
Apos a descoberta da penicilina por Alexander Fleming - em 1940, muitos outros compostos antimicrobianos foram relatados, oriundos de uma ampla variedade de invertebrados, plantas e especies de animais - os antimicrobianos, como: tirocidina, gramidicina, bombinina, melitina, surgindo assim a gera antibioticah. Em seguida, no intuito de obter candidatos para a geracao de novas drogas, particularmente no combate aos virus, fungos e bacterias, o foco na obtencao de peptideos antimicrobianos tambem passou a serem bacterias, fungos, insetos, aracnideos, crustaceos, anfibios, peixes e plantas. Nesse contexto, um peptideo antimicrobiano, denominado IsCT foi isolado em 2001, em Isalo-Madagascar, durante a analise do veneno do escorpiao Opisthacanthus madagascariensis, o qual apresentou atividade hemolitica e antimicrobiana. No intuito de obter-se melhores resultados biologicos contra os agentes patogenicos e uma baixa toxicidade, esse trabalho propoe o desenho de novos analogos do IsCT, com a substituicao de alguns residuos de aminoacidos da sua sequencia, levando-se em consideracao o balanco hidrofobico, carga e sua anfipaticidade. Todos os peptideos foram sintetizados em fase solida, pela estrategia Fmoc, purificados em Cromatografia Liquida de Alta Eficiencia (HPLC) e caracterizados por Espectrometria de Massas (LC/ESI-MS); alem disso, foram realizados estudos conformacionais por Dicroismo Circular (CD).Os ensaios biologicos foram realizados em bacteria gram-negativa Escherichia coli (SBS 363), bacteria gram-positiva, Micrococcus luteus (A270) e a levedura Candida albicans (MDM8). Alem disso, foram realizados ensaios hemoliticos e de degradacao enzimatica. Os resultados demonstraram que o analogo 6foi duas vezes mais ativo que o IsCT nativo, nos tres micro-organismos testados, enquanto que os analogos 1, 5 e 8 mostraram-se menos ativos. Em contrapartida, os analogos 2, 3, 6, 7 e 9 apresentaram um aumento na atividade hemolitica. Todos os analogos apresentaram uma rapida cinetica de degradacao, contudo verifica-se que as substituicoes na posicao 8 (analogo 3 e 4) propiciaram analogos um pouco mais resistentes as proteases. Nos Estudos de CD observa-se que a maioria dos analogos apresenta uma propensao a ¿¿-helice em meios com 50% de TFE e 10 ¿ÊM de SDS, como relatado para o IsCT nativo. De forma geral, com os resultados obtidos dos dez peptideos sintetizados neste trabalho foi possivel obter dois peptideos - [Lys]3-IsCT e [Ala]1[Phe]5[Lys]8-IsCT - os quais mostraram uma reducao do 50% na inibicao dos micro-organismos testados, uma menor atividade hemolitica, em comparacao com o IsCT (peptideo nativo), alem de serem mais resistentes a degradacao proteolitica, indicando que as alteracoes pontuais no IsCT afetam significativamente a sua atividade biologica, hemolitica e a sua estruturacao, tornando-os possiveis candidatos a novas drogas.
After the discovery of penicillin by Alexander Fleming in 1940, many other antimicrobial compounds (as tyrocidine, gramicidin, bombin and melittin) were described from a wide variety of plants, invertebrates and other animal species, providing the Antibiotic Era. Then, in order to obtain candidates for the generation of new drugs, particularly in the fight against viruses, fungi and bacteria, focus on obtaining antimicrobial peptides also came to be bacteria, fungi, insects, arachnids, crustaceans, amphibians, fishes and plants. In this sense, an antimicrobial peptide called IsCT was isolated in 2001 in Isalo Madagascar, while analyzing the venom of the scorpion Opisthacanthus madagascariensis that showed hemolytic and antimicrobial activity. In the attempt to obtain better biological outcomes against pathogens and a low toxicity, this study proposes the design of new IsCT analogues, with the replacement of some amino acids of its original sequence, considering its hydrophobic balance, charge and amphipathicity. All peptides were synthesized on solid phase, by Fmoc strategy, purified by high-performance liquid chromatography (HPLC) and characterized by mass spectrometry (LC / ESI-MS); furthermore, were performed conformational studies by circular dichroism (CD). The biological tests were carried out on gram-negative bacteria Escherichia coli (SBS 363), gram-positive bacteria Micrococcus luteus (A270) and Candida albicans (MDM8). In addition, enzymatic degradation and hemolytic assays were performed. The results showed that the analog 6 was twice more active than native IsCT in three micro-organisms tested, whereas the analogs 1, 5 and 8 were less active. In contrast, the analogous 2, 3, 6, 7 and 9 exhibited an increase in hemolytic activity. All analogs exhibited rapid degradation kinetics, however, it is seen that substitutions at positions 8 (analogous 3 and 4) analogs provided a little more resistant to proteases. In the CD studies, was observed that most analogs presents a propensity for á-helix in solutions with 50% TFE and 10 ìM of SDS, as has been reported for the native IsCT. In general, the results obtained from the ten peptides synthesized in this work it was possible to obtain two peptides to [Lys]3-IsCT and [Ala]1[Phe]5[Lys]8-IsCT which showed a reduction of 50% in inhibition of tested microorganisms, a lower hemolytic activity compared with the IsCT (native peptide) plus were more resistant to protease degradation, indicating that specific changes in IsCT significantly affect its biological activity, hemolytic and their structure, making the possible candidates for new drugs.
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10

Ribeiro, Mariana Salomé Martins. "Fatores condicionantes das perceções éticas de estudantes do ISCA-UA". Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/12349.

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Mestrado em Contabilidade - Fiscalidade
O conceito de ética, embora abrangente,depende de fatores, como a realidade social do indivíduo e varia com o juízo que cada um de nós tem do que está certo ou errado. Até à atualidade todas as grandes civilizações se debateram com questões desta natureza, adequadas a cada época. Hoje, as questões éticas estão relacionadas essencialmente com comportamentos não éticos no meio empresarial. O objetivo geral deste trabalho, foi perceber as perceções do comportamento ético de alguns estudantes do ISCA-UA e a influência de fatores individuais a fim de perceber a importância da ética, como diferencial positivo para o indivíduo e para a sua profissão. Desta forma, este trabalho pretende dar resposta a questões que podem ou não influenciar a tomada de decisão. Os resultados sugerem que, na escolha de qualidades pessoais, com influência na tomada de decisão ética e nos cenários descritos, particamente não se verificaram diferenças significativas entre os indivíduos, quando agrupados de acordo com os fatores individuais escolhidos. Quando analisámos a relação entre as qualidades pessoais percebidas pelos estudantes e as respectivas respostas à questão “Você teria a mesma atitude?” dos diferentes cenários, a análise por regressão logística estabeleceu correspondência com algumas das qualidades. Estes resultados sugerem que os fatores individuais têm pouca significância neste caso, mas que a importância dada a certas qualidades pessoais esta relacionada com a tomada de decisão ética.
The concept of ethics is comprehensive and ambiguous depending on factors, among others, the social reality of the individual and it variates with the judgment that each of us has on what is right or wrong. Up to the present all great civilizations have struggled with issues of this nature, suited to each historical period. Today, the ethical issues are mainly related to unethical behavior in business area. For this work, the main objective was to understand the perceptions of ethical behavior of students and the influence of individual factors in order to realize the importance of ethics as positive differential for the individual and their profession. Thus, this study aims to answer questions that may or may not influence decision-making. The results suggest that the choices of the personal qualities, with influence in decision-making and ethical scenarios described, virtually no differences were found between subjects when grouped according to individual factors chosen. When we looked at the relationship between personal qualities perceived by students and their responses to the question "Would you have the same attitude?" Of different scenarios, logistics regression analysis established correspondence with some of the qualities. These results suggest that the individual factors have little significance in this case, but the importance given to these personal qualities is related to ethical decision.
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Libros sobre el tema "ISCAT"

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Richard J. Brewer. Caerleon-Isca. Caerleon: The Museum, 1987.

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Gilani, Fawzia. Ismat ki eid. Chennai: Tulika Publishers, 2007.

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Chughtai, Ismat. Ismat ke behtin afsane. Lahore: Book Tak, 1992.

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Danilova, Anna. Vykhozhu tebi Ła iskat £. Moskva: E ksmo, 2002.

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Topuz, Gökçen. Medeni usul hukukunda karinelerle ispat. Sıhhiye, Ankara: Yetkin Yayınları, 2012.

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Reck, Rosle. Alles ischt menschlich: Schwabische Gedichte. Reutlingen: Verlag Karl Knodler, 1986.

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Türk vergi hukukunda ispat, delil. Ankara: Adalet Yayınevi, 2012.

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Dīgar ismat rā ʻavaz̤ nakun. Tihrān: Nashr-i Chashmah, 2009.

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Frei-Frei, Anna. Wimmer dr Schnabil gwagsa ischt. Widnau: Dörfler, 1985.

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Jansasoy, Francisco Tandioy. Iscay Huagchucunamada Parlu: Los Dos Huerfanos. s.l: Comite deeucaacion Inga de la Organizacion, 1988.

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Taylor, Richard W. y Vahid Sandoghdar. "Interferometric Scattering (iSCAT) Microscopy and Related Techniques". En Biological and Medical Physics, Biomedical Engineering, 25–65. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21722-8_2.

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Zinn, Claus, Christina Hoppermann y Thorsten Trippel. "The ISOcat Registry Reloaded". En Lecture Notes in Computer Science, 285–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-30284-8_26.

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Kidwai, Saleem. "Ismat Chughtai: Tehri Lakeer (Urdu)". En Same-Sex Love in India, 283–88. New York: Palgrave Macmillan US, 2000. http://dx.doi.org/10.1007/978-1-137-05480-7_41.

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Kidwai, Saleem. "Ismat Chughtai: Tehri Lakeer (Urdu)". En Same-Sex Love in India, 283–88. New York: Palgrave Macmillan US, 2000. http://dx.doi.org/10.1007/978-1-349-62183-5_41.

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Orbe, Ezequiel, Carlos Areces y Gabriel Infante-López. "iSat: Structure Visualization for SAT Problems". En Logic for Programming, Artificial Intelligence, and Reasoning, 335–42. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-28717-6_26.

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Crasborn, Onno y Menzo Windhouwer. "ISOcat Data Categories for Signed Language Resources". En Gesture and Sign Language in Human-Computer Interaction and Embodied Communication, 118–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-34182-3_11.

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Windhouwer, Menzo y Sue Ellen Wright. "Linking to Linguistic Data Categories in ISOcat". En Linked Data in Linguistics, 99–107. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-28249-2_10.

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Aracri, Raffaella M., Roberta Radini, Monica Scannapieco y Laura Tosco. "Using Ontologies for Official Statistics: The Istat Experience". En Current Trends in Web Engineering, 166–72. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-74433-9_15.

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Wright, Sue Ellen, Menzo Windhouwer, Ineke Schuurman y Marc Kemps-Snijders. "Community Efforts Around the ISOcat Data Category Registry". En The People’s Web Meets NLP, 349–73. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-35085-6_13.

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Sharma, Suruchi, Santosh Kumar, Alok Kumar Mishra, D. Vaithiyanathan y Baljit Kaur. "PVT Aware Analysis of ISCAS C17 Benchmark Circuit". En Lecture Notes in Mechanical Engineering, 1199–211. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-8542-5_107.

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Actas de conferencias sobre el tema "ISCAT"

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Jurdova, Dominika, Kristýna Holanová, Milan Vala y Marek Piliarik. "Interferometric scattering (iSCAT) microscopy with optimized reference wave". En Optical Sensors, editado por Robert A. Lieberman, Francesco Baldini y Jiri Homola. SPIE, 2019. http://dx.doi.org/10.1117/12.2522360.

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Christy, Ashton, Najmeh Tavassoli, Alison Bain, Luke Melo y Edward Grant. "Wide-Field Confocal Interferometric Backscattering (iSCAT)-Raman Microscopy". En Novel Techniques in Microscopy. Washington, D.C.: OSA, 2015. http://dx.doi.org/10.1364/ntm.2015.nm4c.4.

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Reina, Francesco, Silvia Galiani, Dilip Shrestha, Christoffer Lagerholm, Erdinc Sezgin, Philipp Kukura, Christian Eggeling y Daniel Cole. "Interferometric scattering (iSCAT) microscopy: studies of biological membrane dynamics". En Single Molecule Spectroscopy and Superresolution Imaging XI, editado por Jörg Enderlein, Ingo Gregor, Zygmunt K. Gryczynski, Rainer Erdmann y Felix Koberling. SPIE, 2018. http://dx.doi.org/10.1117/12.2289501.

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Nicoli, Francesca, Katharina Konig, Mahyar Dahmardeh, Andre Gemeinhardt, Reza Gholami Mahmoodabadi, Houman Mirzaalian Dastjerdi, Harald Kostler y Vahid Sandoghdar. "Opportunities and Challenges of Single Protein Detection with iSCAT". En 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC). IEEE, 2019. http://dx.doi.org/10.1109/cleoe-eqec.2019.8872414.

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Sandoghdar, Vahid. "Interferometric detection of scattering (iSCAT): from sensing to microscopy". En Biomedical Applications of Light Scattering XI, editado por Adam Wax y Vadim Backman. SPIE, 2021. http://dx.doi.org/10.1117/12.2583829.

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Sandoghdar, Vahid. "Interferometric Scattering Microscopy (iSCAT): seeing and tracking nanoparticles (Conference Presentation)". En Quantitative Phase Imaging VI, editado por Gabriel Popescu, YongKeun Park y Yang Liu. SPIE, 2020. http://dx.doi.org/10.1117/12.2552983.

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Čičala, Martin, Milan Vala, Lukasz Bujak y Marek Piliarik. "Noise characteristics and localisation precision in ultrafast interferometric scattering (iSCAT) microscopy". En Optical Sensors, editado por Robert A. Lieberman, Francesco Baldini y Jiri Homola. SPIE, 2019. http://dx.doi.org/10.1117/12.2522407.

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Bujak, Lukasz y Marek Piliarik. "Interferometric scattering (iSCAT) microscopy for high fidelity tracking at microseconds timescales". En Nanoimaging and Nanospectroscopy VI, editado por Prabhat Verma y Alexander Egner. SPIE, 2018. http://dx.doi.org/10.1117/12.2321086.

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Gemeinhardt, Andre, Katharina Konig, Francesca Nicoli, Mahyar Dahmardeh y Vahid Sandoghdar. "Visualizing Cellular Secretion with Single-Protein Sensitivity via Interferometric Scattering Microscopy (iSCAT)". En 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC). IEEE, 2019. http://dx.doi.org/10.1109/cleoe-eqec.2019.8872994.

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Celik, Yunus, Dylan Powell, Wai Lok Woo, Samuel Stuart y Alan Godfrey. "A feasibility study towards instrumentation of the Sport Concussion Assessment Tool (iSCAT)". En 2020 42nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) in conjunction with the 43rd Annual Conference of the Canadian Medical and Biological Engineering Society. IEEE, 2020. http://dx.doi.org/10.1109/embc44109.2020.9175656.

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Informes sobre el tema "ISCAT"

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Scheel, Hans J. ISCGT-1 First International School on Crystal Growth Technology. Fort Belvoir, VA: Defense Technical Information Center, septiembre de 1998. http://dx.doi.org/10.21236/ada354428.

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Selby, Sarah. Climate & environment assessment: Business case for support to the International Security Advisory Team (ISSAT). Evidence on Demand, junio de 2014. http://dx.doi.org/10.12774/eod_hd.june2013.selby.

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Ghislandi, Simone, Raya Muttarak, Markus Sauerberg y Benedetta Scotti. Human costs of the first wave of the COVID-19 pandemic in the major epicentres in Italy. Verlag der Österreichischen Akademie der Wissenschaften, julio de 2021. http://dx.doi.org/10.1553/populationyearbook2022.res2.1.

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Deaths from COVID-19 can be miscounted due to under-reporting and inaccurate death registration. Mortality is often reported at the national level, which can result in the underestimation of the true scale of the impact of the pandemic since outbreaks tend to be localised. This study exploits all-cause daily death registration data provided by the Italian Statistical Office (ISTAT) from 1 January to 31 October to estimate the excess mortality and the corresponding changes in life expectancy during the first wave of the COVID-19 pandemic. Focusing on the five most severely hit provinces in Italy (Bergamo, Brescia, Cremona, Lodi and Piacenza), we calculate the excess mortality in 2020 compared to the average mortality of the years 2015 to 2019. Moreover, we estimate the excess mortality in the first quadrimester of 2020, and the annual life expectancy at birth. The estimated excess deaths show that during this period, mortality was significantly higher than the official mortality statistics for COVID-19. According to our estimates for the first quadrimester, life expectancy in the five provinces declined by 5.4 to 8.1 for men and by 4.1 to 5.8 years for women. In addition, we find that annual life expectancy decreased by 2.4 to 4.1 years for men and by 1.9 to 2.8 years for women compared to the 2015–2019 average. Thus, we conclude that the first wave of the COVID-19 pandemic had a substantial impact on population health in the hardest hit areas in Italy.
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