Literatura académica sobre el tema "Hydrolysats de protéines – Purification"
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Artículos de revistas sobre el tema "Hydrolysats de protéines – Purification":
de Boissieu, D., F. Ammar y C. Dupont. "L'allergie aux hydrolysats de protéines". Revue Française d'Allergologie et d'Immunologie Clinique 40, n.º 1 (enero de 2000): 98–104. http://dx.doi.org/10.1016/s0335-7457(00)80031-3.
Pecquet, C. y M. Laurière. "Hydrolysats de protéines du blé : nouveaux allergènes". Revue Française d'Allergologie et d'Immunologie Clinique 43, n.º 1 (enero de 2003): 21–23. http://dx.doi.org/10.1016/s0335-7457(02)00010-2.
Juchet, A., F. Rancé, A. Broue, F. Bremont y G. Dutau. "Intolérance aux hydrolysats de protéines chez l'enfant". Revue Française d'Allergologie et d'Immunologie Clinique 33, n.º 4 (octubre de 1993): 313–14. http://dx.doi.org/10.1016/s0335-7457(05)80052-8.
Siala, N., I. Fetni, O. Azzabi, O. Rebah, S. Briki, M. Ben Hariz y A. Maherzi. "Allergie aux hydrolysats de protéines de lait de vache". Revue Française d'Allergologie 53, n.º 3 (abril de 2013): 336. http://dx.doi.org/10.1016/j.reval.2013.02.015.
de Boissieu, D. y C. Dupont. "Allergie aux hydrolysats de protéines du lait chez l'enfant". Archives de Pédiatrie 14, n.º 1 (enero de 2007): 124–26. http://dx.doi.org/10.1016/j.arcped.2006.10.006.
Ibsaine, O., K. Djenouhat, H. Berrah y Z. Arrada. "P-496 – Allergie aux hydrolysats de protéines chez l'enfant". Archives de Pédiatrie 22, n.º 5 (mayo de 2015): 359. http://dx.doi.org/10.1016/s0929-693x(15)30672-2.
Ammar, F., D. de Boissieu y C. Dupont. "Allergie aux hydrolysats de protéines. À propos de 30 cas". Archives de Pédiatrie 6, n.º 8 (agosto de 1999): 837–43. http://dx.doi.org/10.1016/s0929-693x(00)88476-6.
Lahouel, N., O. Kheroua, F. Mezemaz y D. Saidi. "Évaluation de l’activité antigénique des hydrolysats de protéines du lactosérum camelin". Revue Française d'Allergologie 56, n.º 6 (octubre de 2016): 471–76. http://dx.doi.org/10.1016/j.reval.2015.12.001.
RIGOURD, V., J. MAGNY, A. AYACHI, M. DUBOIS, C. DECHILLAZ, M. VODOVAR, N. MEDEJEL, D. ANDRIAMANAMIRIJA, C. SLABA y M. VOYER. "Allergie néonatale aux hydrolysats poussés de protéines de lait de vache". Revue Française d'Allergologie et d'Immunologie Clinique 40, n.º 2 (marzo de 2000): 185–89. http://dx.doi.org/10.1016/s0335-7457(00)80006-4.
Olaiwan, A., C. Pecquet, P. Mathelier-Fusade y C. Francès. "Urticaire de contact aux hydrolysats de protéines de blé contenus dans des cosmétiques". Annales de Dermatologie et de Vénéréologie 137, n.º 4 (abril de 2010): 281–84. http://dx.doi.org/10.1016/j.annder.2010.01.010.
Tesis sobre el tema "Hydrolysats de protéines – Purification":
Kobbi, Sabrine. "Purification de la RuBisCO à partir de la Luzerne, hydrolyse enzymatique, identification, structure-fonction des peptides bioactifs et leur valorisation dans des produits alimentaires". Electronic Thesis or Diss., Lille 1, 2017. http://www.theses.fr/2017LIL10201.
Alfalfa is an excellent source of protein. However, RuBisCO proteins showed most interest. Indeed, this protein has been labelled the most abundant on earth; it constitutes about 65% (w/w) of soluble leaf protein of Alfalfa. In this work, a new method was introduced for the purification of RuBisCO from alfalfa powder 10% (w /v), using two different solvents and pH effect. In a first step, the performance of the proposed RuBisCO recovery method was evaluated through qualitative and quantitative analysis and the results obtained showed that this new method could replace some conventional industrial processes. In a second step, enzymatic hydrolysis was carried out on the purified RuBisCO, which resulted in a large bioactive peptide population. The final peptides after 24h of hydrolysis showed better antibacterial or antioxidant activity compared to the other peptide hydrolysates. Nine new antibacterial peptides have been identified and characterized by MS and have a MIC of 2-6 mM against four species of bacteria: B subtilis, E coli, L innocua and M luteus. In addition, antioxidants peptide fractions were identified in this work, their antioxidant activity was evaluated by various in vitro and in vivo tests on oil of Colza. Finally, the addition of peptide RDRFL derived from the peptic hydrolysis of RuBisCO has a positive effect on the prolongation of the shelf life of minced meat and of tomato puree
Tauzin, Jérôme. "Biofonctionnalités de peptides issus de caséines αs bovines : Cinétique d'hydrolyse trypsique de la caséine αs2 et activité inhibitrice de l'ECA des peptides". Nancy 1, 2003. http://www.theses.fr/2003NAN10224.
αS2-Casein is the less studied substrate to obtain bioactive peptides among the major milk proteins. After its purification by ion exchange chromatography followed by hydrophobic interactions chromatography, it was hydrolysed by trypsin and resulting peptides were identified. Their release kinetics revealed three areas having different susceptibility to proteolysis. These data and secondary structure prediction helped to define a hypothetical model of protein organisation in solution. Four tryptic peptides inhibited angiotensin-I converting enzyme (CEI) with IC50 values comprised between 4 and 15 micro M. Their sequences were confronted with others inhibitors to discuss sequence-activity relationships
Kobbi, Sabrine. "Purification de la RuBisCO à partir de la Luzerne, hydrolyse enzymatique, identification, structure-fonction des peptides bioactifs et leur valorisation dans des produits alimentaires". Thesis, Lille 1, 2017. http://www.theses.fr/2017LIL10201.
Alfalfa is an excellent source of protein. However, RuBisCO proteins showed most interest. Indeed, this protein has been labelled the most abundant on earth; it constitutes about 65% (w/w) of soluble leaf protein of Alfalfa. In this work, a new method was introduced for the purification of RuBisCO from alfalfa powder 10% (w /v), using two different solvents and pH effect. In a first step, the performance of the proposed RuBisCO recovery method was evaluated through qualitative and quantitative analysis and the results obtained showed that this new method could replace some conventional industrial processes. In a second step, enzymatic hydrolysis was carried out on the purified RuBisCO, which resulted in a large bioactive peptide population. The final peptides after 24h of hydrolysis showed better antibacterial or antioxidant activity compared to the other peptide hydrolysates. Nine new antibacterial peptides have been identified and characterized by MS and have a MIC of 2-6 mM against four species of bacteria: B subtilis, E coli, L innocua and M luteus. In addition, antioxidants peptide fractions were identified in this work, their antioxidant activity was evaluated by various in vitro and in vivo tests on oil of Colza. Finally, the addition of peptide RDRFL derived from the peptic hydrolysis of RuBisCO has a positive effect on the prolongation of the shelf life of minced meat and of tomato puree
Albe, Slabi Sara. "Développement et optimisation d'un procédé extrapolable de production d'isolats de protéines de tournesol". Electronic Thesis or Diss., Université de Lorraine, 2019. http://www.theses.fr/2019LORR0167.
Sunflower meal, by-produced after oil extraction process, is a valuable source of proteins (30−70% on dry matter basis). These proteins are composed of two main fractions: globulins (helianthinins) and albumins (SFA). Thanks to well-balanced amino acid composition and good functional properties, they are considered very promising for human nutrition as protein isolates. However, the literature shows many scientific drawbacks during sunflower protein extraction and purification. These drawbacks lie on interaction between proteins and chlorogenic acid (major hydrosoluble phenolic compound of sunflower), poor extraction yield and helianthinin denaturation during protein purification by acidic precipitation. The goal of this thesis work was to overcome these limitations and to propose a scalable process for production of sunflower protein isolates. The first part of the thesis was based on the development of a new method for simultaneous quantification of proteins, free chlorogenic acid isomers and chlorogenic acid bound to proteins. This analytical tool provided fast and reliable access to performance criteria crucial for further development and optimization of sunflower protein production process. In the second part of the thesis, an optimal condition for extraction of total proteins from sunflower meal allowing maximizing extraction yield and minimizing protein-phenol interaction was searched. For this purpose, multicriteria optimization based on modelling by design of experiments and genetico-evolutionary algorithms was applied. Then, an alternative method for protein purification by ultrafiltration was developed. This part of study has improved the global understanding of sunflower protein extraction process and yielded in a satisfactory product. However, the residual meal produced after protein extraction was poor in proteins and rich in phytic acid (antinutritional factor). The third part of the thesis was therefore focused on the implementation of an alternative strategy of selective extraction of albumins. To do so, the methodology of modelling and multicriteria optimization, used in second part of the thesis, allowed to identify the optimal conditions for selective extraction of albumins with good yield keeping a satisfactory value of the residual meal. The extracted albumins were light-coloured, rich in sulphur-containing amino acids and more soluble than total sunflower proteins. The functional properties of albumins (foaming, emulsifying) were improved or comparable to those of soy proteins. Therefore, the established strategy provided a sustainable process for production of albumins that would be used in human nutrition and residual meal for feed applications
Abou-Diab, Mira. "Production éco-circulaire de peptides antibactériens, antifongiques et antioxydants déminéralisés à partir d'hémoglobine bovine par électrodialyse avec membranes bipolaires : étude de faisabilité, mécanisme enzymatique, optimisation des paramètres, comparaison avec l'hydrolyse conventionnelle et prévention du colmatage". Electronic Thesis or Diss., Université de Lille (2018-2021), 2021. http://www.theses.fr/2021LILUR031.
Bovine cruor, a slaughterhouse waste, is produced in large quantities all around the world. This co-product was mainly composed of hemoglobin, a protein rich in bioactive peptides after its enzymatic hydrolysis. However, during conventional hydrolysis, chemical agents are necessary to adjust/regulate the pH of the solution and the final hydrolysates produced contain high levels of mineral salts. Therefore, in this study, it is proposed to apply, for the first time, a green technology, named electrodialysis with bipolar membrane (EDBM), as an alternative method to the conventional enzymatic hydrolysis of hemoglobin to obtain purified bioactive peptides. The main objectives of the present thesis were to test the feasibility of this new process to produce bioactive peptides from bovine hemoglobin, to establish the optimal conditions, to avoid membrane fouling and to apply a new original « multiple-step » EDMB process allowing the production of demineralized bioactive peptides without the addition of chemical salts. Bipolar/monopolar (anionic or cationic) configurations using the H+ and OH- generated by the bipolar membranes to regulate the pH were investigated and compared to a conventional process using chemical acid and base. The EDBM configuration formed with cationic membranes allowed the production of hydrolysates containing a low concentration of mineral salts but with fouling formation on the cationic membrane, while EDBM configuration formed with anionic membranes allowed the production of hydrolysates without fouling but with a similar salt concentration than the control. Based on these results, a new 3 compartments EDBM configuration was carried-out for denaturing the hemoglobin, inactivating the enzymatic reaction and demineralizing up to 85% the hemoglobin hydrolysate simultaneously. However, a fouling was still observed on the anionic membrane due to hem precipitation. For this reason, an additional step of discoloration was tested before the demineralization to avoid fouling using the electrogenerated acid. The discolored and demineralized peptides recovered showed antioxidant activity, antibacterial activity against many bacterial strains (Gram + and Gram -) and for the first time antifungal activity against many molds and yeasts strains. Moving towards a circular economy, this sustainable technology has found to be effective in performing multiple operations simultaneously and has a great potential for industrial hydrolysis of blood, since it produces purified biopeptides with a low mineral content and can be used as natural preservatives on meat
Rulence, Alexandre. "Mise en œuvre de procédés membranaires pour la séparation sélective de la nisine à partir de surnageants de culture complexes". Electronic Thesis or Diss., Université de Lille (2022-....), 2023. https://pepite-depot.univ-lille.fr/ToutIDP/EDSMRE/2023/2023ULILR034.pdf.
Nisin, a bacteriocin produced by lactic acid bacteria (LAB) presents physicochemical properties such as a thermal resistance and an antimicrobial activity against food pathogens bacteria. Nisin is actually the only bacteriocins labelled as Generally Recognized As Safe (GRAS) by the U.S Food and Drug Administration (FDA) and is thus the only bacteriocin used as a natural preservative in the food industry, making it an interesting alternative to the use of chemical preservatives. However, its uses are hampered at industrial scale due to low yields et high cost linked to its production on commercial broth and its purification necessity the combination of low yields techniques such as salting out coupled with chromatography.In this case we investigated in this work the use of food grade by-product produces by the food industry in replacement of costly commercial broth. Several by-products composed of vegetal and fish peptones were tested for the production of nisin. Whey being the most used by-product employed for production of nisin and several bacteriocin, we tested and compared different vegetal and fish proteins hydrolyzates regarding biomass production and nisin yields obtained. Several vegetal and fish protein hydrolyzates were tested with two different strains of Lactococcus lactis in order to optimize nisin production. Results showed a greater nisin production using L.lactis UL 719 when compared to a commercial strain. Results also showed the efficacy of some vegetal and one fish by-product for the production of nisin when compared to whey medium and commercial broth MRS. During this work was also investigating alternatives for nisin purification. Electro- and pressure-driven membrane process were studied for nisin purification and especially ultrafiltration (UF) and electrodialysis for which no literature reported the use of ED for nisin purification. ED was applied to the purification of nisin from a commercial solution and from a cell-free supernatant produced with whey permeate as broth for fermentation. UF was applied to the purification of nisin from a cell-free supernatant and permit to compare UF and ED in this application. This work enables us to demonstrate nisin interaction with ion exchange membrane never reported and enable its purification with purification factor comparable to conventional method actually used. Moreover, we demonstrated the use of ED not only efficient for nisin purification but with the possibility to implement ED in an eco-circularity concept, from nisin production using by-products, to its purification with ED and the recycling of salts from saline effluent produced during nisin salting-out
Le, Coeur Catherine. "Contribution à l'étude d'un hydrolysat pepsique de myoglobine de muscle squelettique rouge de thon Thunnus Albacares : caractérisation des peptides issus de l'hydrolyse étude de l'association hème-peptide". La Rochelle, 1996. http://www.theses.fr/1996LAROS011.
Ravallec, Rozenn. "Valorisation d'hydrolysats d'origine marine : optimisation de la concentration en peptides apparentés aux facteurs de croissance et aux agents sécrétagogues : essais in vitro et in vivo". Brest, 2000. http://www.theses.fr/2000BRES2041.
Chabanon, Gérald. "Hydrolyses enzymatiques d'isolats protéiques issus de tourteaux de colza : cinétique, modélisation, caractérisation et fonctionnalité des peptides". Vandoeuvre-les-Nancy, INPL, 2005. http://docnum.univ-lorraine.fr/public/INPL/2005_CHABANON_G.pdf.
This thesis made it possible to study obtaining biologically active peptides or with functional properties through the development of processes for the preparation and the hydrolysis of protein isolates resulting from rapeseed cakes. First, a method of preparation of two protein isolates being different by the type of proteins (Globulin or Albumin) was developed. The two isolates do not have good functional properties but their partial hydrolysis by Alcalase 2. 4L improves some of them. Then, the hydrolytic action of commercial proteases (Alcalase 2. 4L, Pronase SG, Neutrase 0. 8L, Prolyve BS, Lypaïne 6500, Orientase 90N, Espérase 7. 5L) on the isolate of globulins was compared. The valorisation of the hydrolysates related to their capacity to promote the growth of animal cells cultivated in a serum-free medium. It was shown that the kinetics of hydrolysis, the size of produced peptides and the biological activity of the hydrolysates are significantly influenced by the specificity of the enzyme and there is a relation enzyme/ degree of hydrolysis (DH)/ targeted activity. Lastly, we showed for three different enzyme/substrate systems (Alcalase / Globulin, Pronase / Globulin and Alcalase / Albumin) that at given DH and pH, the peptide composition of the hydrolysates is independent of the initial enzyme and substrate concentrations and of the temperature. Thus, the prediction of the temporal evolution of the DH, whatever the values of precedent parameters, allows to control the generation of a peptide mixture with targeted properties. A model based on the reaction pathway of Michaelis-Menten was then built in order to simulate the hydrolysis kinetics in batch reactor. For that, limiting phenomena implied in the hydrolysis (inhibition or inactivation of the enzyme, modification of the substrate) were highlighted
Debrabant, Alain. "Étude de la protéolyse de la protéine P126 de Plasmodium falciparum". Lille 1, 1990. http://www.theses.fr/1990LIL10067.
Libros sobre el tema "Hydrolysats de protéines – Purification":
1944-, Harrison Roger G., ed. Protein purification process engineering. New York: M. Dekker, 1994.
Genie et recherches sur les biotechnologies des proteins. Symposium. Technologies de purification des protéines =: Proteins purification technologies : volume 3, Toulouse 13-15 avril, 1988. Villebon-sur-Yvette: GRBP, 1988.
International Symposium on Blood Protein Biotechnology (2nd 1992 Nancy, France). Biotechnology of blood proteins: Purification, clinical and biological applications = Biotechnologie des protéines du sang : purification, applications cliniques et biologiques. Paris: John Libbey Eurotext, 1993.
Ahmed, Hafiz. Principles and reactions of protein extraction, purification, and characterization. Boca Raton: CRC Press, 2005.
A, Shukla Abhinav, Etzel Mark Raymond y Gadam Shishir, eds. Process scale bioseparations for the biopharmaceutical industry. Boca Raton: CRC/Taylor & Francis, 2007.
Westermeier, Reiner. Electrophoresis in practice: A guide to theory and practice. Weinheim: VCH, 1993.
Westermeier, Reiner. Electrophoresis in practice: A guide to methods and applications of DNA and protein separations. 2a ed. Weinheim: VCH, 1997.
Westermeier, Reiner. Electrophoresis in practice: A guide to methods and applications of DNA and protein separations. 3a ed. Weinheim: Wiley-VCH, 2001.
Saluz, H. P. A laboratory guide for in vivo studies of DNA methylation and protein/DNA interactions. Basel: Birkhäuser Verlag, 1990.
Harrison, Roger. Protein Purification Process Engineering (Biotechnology and Bioprocessing Series). CRC, 1993.