Tesis sobre el tema "Human dermal fibroblasts"
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Junker, Johan. "Human Dermal Fibroblasts in Tissue Engineering". Doctoral thesis, Linköpings universitet, Cellbiologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-19716.
Texto completoJunker, Johan P. E. "Human dermal fibroblasts in tissue engineering /". Linköping : Department of Clinical and Experimental Medicine, Linköping University, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-19716.
Texto completoBoright, Andrew Pepler. "Prolidase deficiency : studies in human dermal fibroblasts". Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75956.
Texto completoMitchell, Stephen Andrew. "The radiation response of human dermal fibroblasts". Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.392193.
Texto completoKashpur, Olga. "Oxygen-mediated basic fibroblast growth factor (FGF2) effects on adult human dermal fibroblasts". Digital WPI, 2015. https://digitalcommons.wpi.edu/etd-dissertations/546.
Texto completoAl-Rikabi, Aaiad H. A. "Impaired Wound Healing and Inflammation: The Role of the Dermal Fibroblast. Phenotypic Changes in the Human Dermal Fibroblast with Inflammation; Potential Impact on Wound Healing". Thesis, University of Bradford, 2019. http://hdl.handle.net/10454/18331.
Texto completoSommar, Pehr. "Differentiation of Human Dermal Fibroblasts and Applications in Tissue Engineering". Doctoral thesis, Linköpings universitet, Hand och plastikkirurgi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-60879.
Texto completoFray, Timothy Richard. "Measuring single cell contractility : comparison of human dermal fibroblasts and myofibroblasts". Thesis, University of York, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298591.
Texto completoKarlsson, Lisa. "Differentiation of Human Dermal Fibroblasts : a New Tool in Vascular Tissue Engineering". Licentiate thesis, Linköpings universitet, Institutionen för klinisk och experimentell medicin, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-20319.
Texto completoIn the printed version the series number of this Licentiate thesis is 98. In the electronic version it has been corrected to 99.
Rockwood, Jananie. "House Dust Mite Induced Gene Expression and Cytokine Secretion by Human Dermal Fibroblasts". Wright State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=wright1347976529.
Texto completoKwok, Hoi Hin. "The anti-aging effects of ginsenosides on human endothelial cells and dermal fibroblasts". HKBU Institutional Repository, 2011. http://repository.hkbu.edu.hk/etd_ra/1235.
Texto completoRodrigues, Annelissa Zorzeto. "Avaliação in vitro do cultivo de fibroblastos gengivais humanos em matriz dérmica acelular". Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-30062008-150532/.
Texto completoAcellular Dermal Matrix, ADM, is a biomaterial that has been used in periodontal procedures to treat mucogingival defects. Mucogingival defects can be corrected by autogenous grafts that are the most common procedure used in periodontology, however, because of the limited source of donor\'s tissue this procedure became limited. The aim of this investigation was to verify, in vitro, different aspects related to human gingival fibroblasts seeding on to the ADM. Human gingival fibroblasts were established from explant cultures from the connective tissue of keratinized gingiva collected from three healthy patients. ADM was seeded with gingival fibroblasts for 14 and 21 days, and then cell adherence, proliferation and viability were analyzed. Results revealed that, at day 7, fibroblasts were adherent and spreading on the ADM surface, and were unevenly distributed, forming a discontinuous single cell layer, at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. The results suggests that fibroblasts seeding on the ADM for 14 days can allow good conditions for cell adhesion and spread on the matrix, however, because of the high collagen fiber bundle density cell, migration inside the matrix was limited.
Nguyen, Elise B. "Electrical Stimulation of Human Dermal Fibroblasts and the Quantification of Collagen, Collagenase, and Elastin". Thesis, California State University, Long Beach, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10263522.
Texto completoElectrical stimulation of tissues has been found to have many uses in pain management, antibacterial treatment, and wound healing. In vivo, it is known to stimulate epidermal migration and increase fibroblast cell proliferation. Here the effects of electrical field (EF) stimulation on collagen, elastin, and collagenase expression in human dermal fibroblasts are studied. The cells are stimulated in bioreactor using square wave voltage pulses controlled by potentiostat for up to 24 h period. The pulse voltage (0–10V), pulse bias (0, +,−), pulse time (10–1000 ms), rest time (0.1–10 s) was varied. The effects of EF stimulation is evaluated in terms of protein expression level and changes in cell morphology. The results show that the expressions of these proteins are correlated and are doubled when EF stimulation larger than 3V and positive bias is applied. The shorter pulse time stimulates the cells more effectively, while the rest time between pulses has smaller effect.
Cunningham, Mary Jane. "The induction and inhibition of benzo(a)pyrene metabolism in human epidermal keratinocytes and dermal fibroblasts /". The Ohio State University, 1985. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487259125219762.
Texto completoKole, Denis. "Role of Fibroblast Growth Factor 2 in Maintenance of Multipotency in Human Dermal Fibroblasts Treated with Xenopus Laevis Egg Extract Fractions". Digital WPI, 2014. https://digitalcommons.wpi.edu/etd-dissertations/207.
Texto completoDeing, Verena Daniela [Verfasser]. "Characterization of the oxytocin system in primary human dermal fibroblasts and keratinocytes / Verena Daniela Deing". Konstanz : Bibliothek der Universität Konstanz, 2013. http://d-nb.info/1043906886/34.
Texto completoArikatla, Venkata Sravya. "Stress-Induced Senescence in Human Dermal Fibroblasts: Effects of Creatine and Nicotinamide Post Stress Treatment". Wright State University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=wright1629899898596448.
Texto completoDahlbom, Moa. "Gene Expression of CTGF in Human Dermal Fibroblasts When Exposed to TGF-β and IL-1α". Thesis, Örebro universitet, Institutionen för läkarutbildning, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-36966.
Texto completoTeves, Joji M. Y., Vedanshi Bhargava, Konner R. Kirwan, Mandi J. Corenblum, Rebecca Justiniano, Georg T. Wondrak, Annadurai Anandhan et al. "Parkinson's Disease Skin Fibroblasts Display Signature Alterations in Growth, Redox Homeostasis, Mitochondrial Function, and Autophagy". FRONTIERS MEDIA SA, 2018. http://hdl.handle.net/10150/626553.
Texto completoMignon, Charles. "Photo-biomodulation of human skin fibroblast sub-populations : a systematic approach for the optimization of optical treatment parameters". Thesis, University of Bradford, 2017. http://hdl.handle.net/10454/16064.
Texto completoJouret, Chantal. "Effects of matrix and phenotype on human dermal fibroblast attachment under laminar shear stress : implications for the development of tissue-engineered heart valves". Thesis, Georgia Institute of Technology, 1997. http://hdl.handle.net/1853/11251.
Texto completoHorobin, Adele Jayne. "Maggots and wound healing : the effects of Lucilia sericata larval secretions upon interactions between human dermal fibroblasts and extracellular matrix proteins". Thesis, University of Nottingham, 2005. http://eprints.nottingham.ac.uk/11516/.
Texto completoGonzales, Christopher R. "3,3′,5′-triido-L-thyronine alters protein kinase B, phosphotase and tensin homolog and connective tissue growth factor expression in human dermal fibroblasts". Thesis, Boston University, 2012. https://hdl.handle.net/2144/12397.
Texto completoCutaneous tissue repair is complex and involves a variety of growth factors to regulate a balance of regeneration and fibrosis during healing. This process is divided into three sequential and overlapping phases: the inflammatory phase, the proliferative phase, and the remodeling phase. Fibroblasts are crucial during this process in that they help initiate inflammatory activity, deposit extracellular matrix proteins for granulation tissue and deconstruct granulation tissue to make way for mature scar formation. Previous studies on the effects of 3,3',5'-triiodo-L-thyronine (T3) on skin have revealed that healing tissue responds to T3 by accelerating skin cell proliferation and migration. These findings indicate that T3 offers potential as a therapeutic drug for individuals with extensive cutaneous damage, chronic skin maladies or retarded wound healing. The mechanisms underlying these changes are not clearly understood, however, elucidation of changes in protein expression patterns should be evaluated to appropriately judge the therapeutic potential of T3. This study aims to characterize T3 dose responsive expression of protein kinase B, phosphatase and tensin homolog, connective tissue growth factor and wnt5a. Western blot analysis and immunodetection revealed that wnt5a is not expressed in human dermal fibroblasts. Protein kinase B did not vary significantly with T3 concentration ranging from 1.0 nM-1.0 1µM, F(4,5)= 1.93, p > 0.05, nor did connective tissue growth factor, F(4,5) = 2.16, p > 0.05. In contrast, phosphatase and tensin homolog showed a statistically significant change in expression, F(4, 15) = 4.67, p less than 0.05. The results presented here provide insight into protein pathways and growth factors through which thyroid hormone produces its effects on the various cells of the integument and suggests that phosphatase and tensin homolog (PTEN) expression levels are responsive to varying concentrations of T3. Future studies should further evaluate the role of T3 on its various targets as a therapeutic option for skin disorders.
Lee-Bellantoni, Margaret S. "Antioxidant defense and redox responses to telomere homolog oligonucleotides in human dermal fibroblasts: a model for investigating redox signaling responses to DNA damage". Thesis, Boston University, 2005. https://hdl.handle.net/2144/37162.
Texto completoPLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
It has been demonstrated that oligonucleotides homologous to the 3' telomere repeat sequence TTAGGG (T-oligos) stimulate DNA damage responses that are also induced by disruption of the telomere loop structure. Adaptive defense against oxidative stress and UV or ionizing radiation has been reported, but adaptive antioxidant defense as a response to mimicking telomere loop exposure has not been described. The T-oligos pTT and pGTTAGGGTTAG were added to human dermal fibroblast cultures to investigate whether mimicking telomere loop disruption stimulates antioxidant defense. pTT stimulated mitochondrial superoxide dismutase protein levels within 72 hours. Cell yields were higher after H202 exposure in fibroblasts pretreated with pTT for 72 hours compared to diluent pretreated cells. Intracellular reactive oxygen species (ROS) levels, measured by flow cytometry and the dichlorofluorescein diacetate probe, increased during T-oligo treatment as compared to diluent and oligonucleotide controls. The time course and degree of ROS stimulation corresponded to the time course for activation and/or induction of p53 and p21/Cip1/Waf1. The NADPH oxidase inhibitor diphenyliodonium chloride abrogated this increase and fibroblasts retrovirally transduced to produce dominant negative p53 failed to display increased ROS, implicating that the T-oligos induced ROS through p53-responsive NADPH oxidases. A horseradish peroxidase assay for extracellular H20 2 showed no H20 2 release with pTT treatment. To determine whether there was induction of senescence, an endpoint response to increased ROS and prolonged T-oligo treatment in fibroblasts, the senescence-associated β-galactosidase assay was conducted in parallel with the DCF assay. Only the 11mer T-oligo treatment modestly increased the number of β-galactosidase positive cells by 72 hours (<30% of cells). This is the first report suggesting that antioxidant defense and ROS signaling are part of the broad adaptive response in mammalian cells presumably initiated by telomere loop disruption and mimicked by T-oligos. T-oligo treatment thus offers a new model for studies of ROS signaling in human dermal fibroblasts, allowing exploration of the relationships between DNA damage, ROS, oxidative stress, and the evolution of cellular defense mechanisms.
2031-01-01
Castellano-Pellicena, Irene. "The role of photoreceptors in human skin physiology; potential targets for light-based wound healing treatments. Identification of opsins and cryptochromes and the effect of photobiomodulation on human skin and in cultured primary epidermal keratinocytes and dermal fibroblasts". Thesis, University of Bradford, 2017. http://hdl.handle.net/10454/16884.
Texto completoMarie Curie ... the CLaSSiC project
Rusciani, Anthony. "Etude du mode de fonctionnement du complexe récepteur de l'élastine : modulation de la composition et de la dynamique de la membrane plasmique". Thesis, Reims, 2012. http://www.theses.fr/2012REIMS019/document.
Texto completoElastin is the matrix protein responsible for the elasticity of tissues where resilience is required such as lung, arteries or skin. Elastin degradation during physiopathological processes produces biologically active peptides named elastin peptides bearing the GXXPG pattern essential for their activity. These peptides regulate various biological functions such as chemotaxis, proteases synthesis and proliferation. These effects are dependent of elastin peptide binding to the elastin receptor complex (ERC). This complex is composed of three subunits: a peripheral protein of 67 kDa called elastin binding protein (EBP) and two membrane-associated proteins, protective protein/cathepsin A (PP/CA) and neuraminidase-1 (Neu-1) of 55 and 61 kDa, respectively. The sialidase activity of Neu-1 is responsible for ERK 1/2 pathway activation following binding of elastin peptide on the elastin receptor complex.In this study, we demonstrate that EBP and lipid rafts colocalize at the plasma membrane. We also show that the disruption of these microdomains and their depletion in glycolipids block the receptor signaling. The use of a monoclonal anti-GM3 blocking antibody shows that this glycosphingolipid is essential for signaling. Following elastin peptide treatment, cellular GM3 level decreases while the lactosylceramide one increases consistently with a GM3/LacCer conversion. The use of lactose or Neu-1 siRNA blocks this process suggesting that the elastin receptor complex is involved in this mechanism. Flow cytometry analysis confirms this elastin peptide-driven LacCer generation.Mass spectrometry analysis of elastin peptide-stimulated cell membrane extracts identified two potentially bioactive lactosylceramides (C23:0 and C24:1) and their synthesis has been realized. Lipid rafts purification by differencial ultracentrifugation in sucrose gradient shows a variation of the microdomains density as well as their identification by fluorescence linked-Dot-blot following elastin peptide stimulation.In vitro biological evaluation of these lactosylceramides shows that they mimic the elastin peptide effects on ERK 1/2 activation, proliferation and MMP-1 synthesis. Finally, ex vivo lactosylceramides evaluation demonstrates a decrease of cardiac tissue necrosis area suggesting that these molecules could be cardioprotective agents. This work proposes an original mechanism of signal transduction at the plasma membrane and let us foresees the elastin receptor complex, elastin peptides and lactosylceramide as new potential therapeutical targets
Gsib, Olfat. "Synthèse et caractérisation d’hydrogels de fibrine et de polyéthylène glycol pour l’ingénierie tissulaire cutanée". Thesis, Compiègne, 2018. http://www.theses.fr/2018COMP2416.
Texto completoOver the past five decades, we assisted in extraordinary advances in the field of skin tissue engineering which led to the in vitro reconstruction of a wide range of skin substitutes. Most of them are dermal substitutes: Their clinical application ranges from treating acute and chronic wounds to soft tissue augmentation. Although increasing numbers of patients have been treated with dermal substitutes, their clinical application has been limited by their substantial cost and some poor healing outcomes. Hence, there is still a challenge to produce a dermal substitute which enhance sufficiently wound healing. To this end, the substitute should exhibit suitable properties for enabling the repair process. Other requirements such as excellent biocompatibility, minimal antigenicity, ease to handle and cost-effective production are also essential. In this context, fibrin hydrogels constitute promising candidates for skin tissue engineering since fibrin fibers form a physiological and provisional backbone during wound healing. However, the poor mechanical properties of fibrin-based hydrogels at physiological concentration are an obstacle to their use. In this study, our aim was to design and characterize mechanically reinforced fibrin-based hydrogels by combining the intrinsic properties of a fibrin network with the mechanical features of a polyethylene glycol network using an interpenetrating polymer network (IPN) architecture. They are intended to be used as dermal scaffolds. The results obtained in this thesis: - Confirmed the suitable physico-chemical properties of IPN, first developed by our partner of the University of Cergy-Pontoise. - Validated their biocompatibility using a three-step approach (in vitro, ex vivo and in vivo assays). - Led to the synthesis and characterization of a new type of fibrin-based macroporous matrices, optimized for 3D dermal fibroblast culture
Grella, Alexandra R. "A mechanism for the FGF2-mediated down-regulation of integrin alpha-11 identified through studying altered adhesome of human dermal fibroblasts undergoing early Mesenchymal-to-Epithelial Transition". Digital WPI, 2015. https://digitalcommons.wpi.edu/etd-dissertations/53.
Texto completoSchneider, Sabine [Verfasser], Jean [Akademischer Betreuer] Krutmann y William [Gutachter] Martin. "Characterization of molecular mechanisms involved in intrinsic and extrinsic skin aging of in situ aged normal human dermal fibroblasts / Sabine Schneider ; Gutachter: William Martin ; Betreuer: Jean Krutmann". Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2021. http://d-nb.info/1241824169/34.
Texto completoAlase, Adewonuola Adelodi. "The role of interleukin-10 family members in inflammatory skin diseases : understanding the mechanism of action of interferon lambda and interleukin-22 on human primary keratinocytes and dermal fibroblasts with a focus on healing responses in inflammatory skin diseases". Thesis, University of Bradford, 2015. http://hdl.handle.net/10454/14303.
Texto completoAlase, Adewonuola A. "The Role of Interleukin-10 Family Members in Inflammatory Skin Diseases. Understanding the mechanism of action of interferon lambda and interleukin-22 on human primary keratinocytes and dermal fibroblasts with a focus on healing responses in inflammatory skin diseases". Thesis, University of Bradford, 2015. http://hdl.handle.net/10454/14303.
Texto completoUniversity of Bradford and Centre for Skin Sciences
Salamito, Mélanie. "Le facteur de transcription antioxydant NRF2 comme nouveau régulateur de la matrice extracellulaire des fibroblastes de peau humaine". Thesis, Lyon, 2020. http://www.theses.fr/2020LYSEN058.
Texto completoThe nuclear factor-erythroid 2-related factor 2 (NRF2) is a transcription factor involved in cell defense against oxidative and xenobiotic stresses. SKN-1, the nematode homologue of NRF2 is a master regulator of longevity that, under specific metabolic conditions, surprisingly acts through the activation of collagens expression. Fibroblasts are the major producers and organizers of collagen-rich tissues and, as such, play a key role in dermis homeostasis. Therefore, we investigated the potential new role of NRF2 in regulating extracellular matrix (ECM) expression in human skin fibroblasts. Dysregulation of NRF2 was realized using siRNA and shRNA. A global transcriptomic analysis of siNrf2 human skin fibroblasts performed by RNA-seq revealed that, in addition to known NRF2 targets, matrisome and tissue skeleton genes were the most represented gene sets, including some key ECM genes. Analysis of ECM production and organization was further conducted in cultured shNrf2 fibroblasts using a combination of microscopies (SHG, confocal, TEM and AFM). Long-term effect of silencing NRF2 in fibroblasts (shNrf2) resulted in defects in collagen expression and fibril formation, likely due to a disturbed collagen I to collagen V ratio. Interestingly, a transcription factor involved in connective tissue disease and described as a regulator of collagen expression was identified as a novel target of NRF2. Immunofluorescence staining of silenced NRF2 fibroblasts (siRNA and shRNA) strikingly revealed that NRF2 downregulation impacts its translocation rate into the nucleus. Our results demonstrate that silencing NRF2 impacts ECM and especially collagens in human skin fibroblasts. A transcription factor known to regulate collagen expression, could act as a specific cofactor of NRF2 in the regulation of ECM gene expression. NRF2 can thus be considered as a novel regulator of ECM genes in human skin fibroblasts and represents a new target to maintain dermis homeostasis
Kamala, Ola. "A Comparison of Cultured Human Dermal Fibroblasts Derived from Terminal and Vellus Hair Bearing Skin. Differences in the expression of inhibitors of apoptosis proteins, oestrogen receptors, and responses to oestradiol under normal and wound induced conditions". Thesis, University of Bradford, 2014. http://hdl.handle.net/10454/13841.
Texto completoTay, Jing Q. "The roles of vitamin D in cutaneous wound healing: In vitro and ex vivo studies of the effect of 1,25(OH)2D3 and its precursors on human dermal fibroblasts and epidermal keratinocytes in cutaneous wound healing". Thesis, University of Bradford, 2018. http://hdl.handle.net/10454/17350.
Texto completoAl-Aasswad, Naser M. I. "An examination of the bioactive lipids involved in skin cell inflammation and in response to ultraviolet radiation : effect of n-3 polyunsaturated fatty acid supplementation on red blood cell and human dermal fatty acid and production of eicosanoids by HaCaT keratinocytes and 46BR.1N fibroblasts following exposure to UVR". Thesis, University of Bradford, 2013. http://hdl.handle.net/10454/14844.
Texto completoHill, Rebecca Philomena. "Human dermal fibroblast responses to inflammatory stress". Thesis, University of Sheffield, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.422116.
Texto completoMaximilien, Jacqueline. "Studies of the impact of core-shell polystyrene nanoparticles on cell membranes and biomimetic models". Thesis, Compiègne, 2015. http://www.theses.fr/2015COMP2180.
Texto completoThis project’s aim was to study polymeric nanoparticle-membrane interactions using both live cells and biomimetic models with the idea to validate such nanoparticles for use in bio-applications. Core-shell polymeric nanoparticles below 100 nm, as this small size is capable of penetrating plasma membranes, were synthesised. Nanoparticles (NPs) with the same chemical composition but with hydrodynamic diameters of ~250 nm, were also prepared in an effort to highlight any effect of NP size on cell internalisation. In this thesis, an innovative method is presented for the synthesis of water-compatible, iniferter-bound polystyrene core shell NPs (~30 nm) using a one-pot synthetic method. A plethora of functionalities could be added to the nanoparticles via shell grafting from the surface of the polystyrene core in the presence of additional monomers via controlled living radical polymerisation. Shell thickness could be tuned as a function of monomer’s concentration and polymerisation time. The nanoparticles were fully characterised by dynamic light scattering, Fourier transform infra-red spectroscopy, microelemental analysis and transmission electron microscopy. Further, the interactions of polystyrene core NPs possessing neutral and anionic shells were investigated using neonatal human epidermal keratinocytes (NHEK), human primary fibroblasts and HaCaT cells. Cytotoxicity studies performed using propidium iodide and lactate dehydrogenase indicated no evidence of cytotoxicity in either cell line. However, cell proliferation monitored by electric cell substrate impedance sensing (ECIS) protocols indicated that anionic nanoparticles induced a dramatic decrease in cell proliferation in keratinocytes. The cellular internalisation of NPs was confirmed by confocal microscopy and no co-localisation was found with early endosomes, lysosomes or actin. Additionally, fluorescence activated cell sorting (FACS) data support the theory that an energy-dependent mechanism is employed for neutral NP internalisation but less so for negatively charged NPs. Biomimetic membrane models were used to investigate specific nanoparticle-lipid interactions under controlled conditions. Employing giant vesicles coupled with fluorescent spectroscopy techniques revealed that core-shell nanoparticles interact deep in the hydrophobic region of bilayers only when the membrane is in the fluid phase. Their mode of entering artificial cells (i.e giant vesicles) appears to cause the formation of pores. Anionic nanoparticles interact with the choline moiety of phosphatidylcholine and confer a rigidifying effect on phosphocholine containing bilayers. Therefore we conclude that the polymeric nanoparticles that we synthesized are versatile tools for cell interaction and imaging studies. These nanomaterials could eventually be applied to drug delivery studies by incorporation of the drug in for instance a thermoresponsive polymeric shell. Furthermore, it is clear that NPs coated with anionic and neutral polymeric shells present a lower toxicity profile than previously reported cationic nanoparticles. Both nanoparticles increase the order lipid bilayer vesicles composed of POPC (the most common glycerophospholipid) in animal and plants. Anionic nanoparticles in particular exhibit a rigidifying effect on POPC lipid bilayers and their mode of entry into cells may be due to the formation of pores which was determined to not induce cell death
Souto, Luis Ricardo Martinhão. "Modelo de pele humana (derme + epiderme) reconstruida in vitro". [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/313309.
Texto completoDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: A obtenção de uma pele humana que apresente derme e epiderme, reconstruída a partir de células isoladas de pacientes, possibilita a realização de enxertos autólogos de pele reconstruída em laboratório (in vitro) em pacientes com áreas doadoras escassas além de permitir ensaios com substâncias químicas e drogas in vitro e não mais in vivo. A partir da cultura de fibroblastos humanos, é possível obter um número suficiente de células que podem ser injetadas em uma matriz de colágeno bovino tipo I que, mantida imersa em meio de cultura, específico para fibroblastos, permite a formação de uma derme humana reconstruída in vitro. Sobre essa derme, através de cultura de queratinócitos e melanócitos humanos, forma-se uma epiderme diferenciada levando à formação de uma pele humana reconstruída in vitro, constituída de derme e epiderme associadas. Essa pele humana formada é, histologicamente, semelhante à pele humana in vivo. Na derme, identifica-se o tecido colágeno, com suas células, e a matriz extracelular organizados paralelamente à epiderme. Esta se desenvolve em várias camadas. Não há distinção entre derme e epiderme no experimento controle, onde não foi utilizado o colágeno bovino tipo I
Abstract: The technique to obtain human skin presenting dermis and epidermis reconstructed from cells isolated from patients allows the performance of autologous grafts of skin reconstructed in laboratory (in vitro) on patients with scarce donor sites, in addition to permitting trials with chemical substances and drugs no more in vivo, but in vitro. It is possible to obtain a sufficient number of cells from human fibroblast culture that can be injected in bovine collagen type I matrix and kept submerged in a specific culture medium for fibroblasts. This will permit the formation of human dermis reconstructed in vitro. On this dermis, through culture of human keratinocytes and melanocytes, a differentiated epidermis is formed, leading to the creation of human skin reconstructed in vitro, composed of associated dermis and epidermis. This human skin is histologically formed in the same way as human skin in vivo. Collagen tissue can be identified in the dermis, with its cells and extracellular matrix organized in parallel to the epidermis, which is developed in several layers
Mestrado
Patologia Clinica
Mestre em Ciências Médicas
Bain, Peter A. y n/a. "Gene Expression Profiling of Cylindrospermopsin Toxicity". Griffith University. School of Biomolecular and Physical Sciences, 2007. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20080404.145834.
Texto completoBain, Peter A. "Gene Expression Profiling of Cylindrospermopsin Toxicity". Thesis, Griffith University, 2007. http://hdl.handle.net/10072/367068.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Physical Sciences
Faculty of Science, Environment, Engineering and Technology
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Marí, Buyé Núria. "Design and development of biomimetic surfaces and three-dimensional environments to study cell behavior". Doctoral thesis, Universitat Ramon Llull, 2012. http://hdl.handle.net/10803/81111.
Texto completoLa biomimètica o biomimetismo son términos que simbolizan el concepto “aprender de la naturaleza”, es decir, aprender de sus sistemas, procesos y modelos, y utilizarlos como fuente de inspiración para solucionar problemas del hombre. El biomimetismo es actualmente un concepto recurrente en el área de ingeniería de tejidos y de este surgen ideas para obtener plataformas más elegantes y sofisticadas que puedan mimetizar mejor las interacciones entre las células y su ambiente. La presente tesis se centra en desarrollar modelos, tanto en dos como en tres dimensiones, mediante la recreación de uno o más factores que caracterizan el ambiente natural de la célula y que tienen su rol importante en el comportamiento celular. Se conoce que tanto las propiedades químicas como mecánicas de la matriz extracelular influyen en las funciones celulares. Debido a esto, se diseñó un nuevo film polimérico que pudiera combinar un hidrogel, con propiedades mecánicas variables, con un monómero reactivo, capaz de inmovilizar biomoléculas. Debido a la complejidad del polímero diseñado, fue necesario recurrir a una técnica de polimerización superficial muy versátil como es la deposición química iniciada en fase vapor (más conocida por su acrónimo en inglés iCVD). Los polímeros fueron ampliamente caracterizados y se corroboró que podían ser modificados con pequeñas biomoléculas como péptidos señalizadores. Las superficies resultantes son bioactivas y permiten la adhesión de células endoteliales. Se obtuvieron otro tipo de superficies biomiméticas relevantes en el ámbito de la ingeniería de tejidos de hueso, a partir de una hidroxiapatita sintetizada por el método sol-gel sumergiéndolas en diferentes medios fisiológicos. La disolución y posterior reprecipitación de los iones proporcionan una capa de apatita con una composición similar a la que se encuentra in vivo. Los experimentos evidencian la importancia de partir de un material relativamente soluble. Precisamente debido a esto la hidroxiapatita pura no es capaz de inducir la precipitación de esta apatita biomimética in vitro. Varios investigadores han relacionado la capacidad de formar apatita con la bioactividad del material, entendiendo bioactividad como la habilidad de estos materiales de promover la unión con el hueso. De todos modos, en ingeniería de tejidos, es necesario un ambiente tridimensional para generar un tejido artificial. Se ha desarrollado un nuevo modelo basado en el uso de un gel blando para obtener tejido duro como el del hueso. Aunque estos conceptos pueden parecer contradictorios, las células adquieren la habilidad de estirarse rápidamente y de formar una densa red celular dentro de este gel tan poco restrictivo desde un punto de vista mecánico. La consiguiente contracción del sistema acaba formando un constructo mucho más pequeño y resistente. Este es un sistema biomimético ya que promueve una gran interacción celular y también la condensación de las células, eventos que también ocurren durante el desarrollo de hueso y cartílago. El modelo se caracterizó extensamente con células osteoprogenitoras MC3T3-E1 que se diferenciaron bajo inducción química. Además, se demostró que el microambiente tridimensional podía promover la expresión espontánea de marcadores osteogénicos. Debido a las interesantes propiedades del sistema, el mismo modelo se usó para inducir la diferenciación condrogénica de fibroblastos dermales humanos. Este tipo celular no ha sido demasiado explorado en ingeniería de tejidos, a pesar de que puede tener un gran potencial en terapia regenerativa. Este trabajo proporciona pruebas de la capacidad condrogénica de estas células en el sistema tridimensional previamente desarrollado.
Biomimetics or biomimicry are terms that imply “learning from nature”, from its systems, processes and models, in order to use nature as inspiration to solve human problems. In tissue engineering, biomimetics is nowadays a recurrent term and a source of ideas to obtain more elegant and sophisticated platforms that could better mimic the interactions between cells and their environment. This thesis is focused on developing models both in two- and three-dimensions by recreation of one or more factors of the cell natural environment that are known to play an important role in cell behavior. Since both the chemical and mechanical properties of the extracellular matrix are known to effectively influence cell function, an innovative polymeric thin film was designed combining a hydrogel with tunable mechanical properties and a reactive molecule, capable to immobilize biomolecules. Due to the complexity of the polymers, a versatile technique such as initiated chemical vapor deposition (iCVD) was required for the synthesis. Extensive characterization revealed that nanostructured hydrogels were obtained and that small biomolecules, such as signaling peptides, could be attached on the surface. The final surfaces are bioactive and support endothelial cell attachment. Relevant biomimetic surfaces for bone tissue engineering could also be obtained from a sol-gel synthesized hydroxyapatite after immersion in different physiological media. The dissolution and posterior reprecipitation of the ions rendered a final apatite layer with a composition similar to that found in vivo. The experiments evidenced the importance of starting from a rather soluble material and, thus, pure hydroxyapatite was not able to promote apatite precipitation in vitro. This capacity has been related to the material bioactivity by many researchers in terms of its ability to bond to bone in tissue engineering applications. However, for tissue engineering a three-dimensional environment is required to build tissue-like constructs. A new model was developed based on the use of a very soft gel to obtain hard tissue. Although the concepts might seem to work in opposite directions, cells gain the ability to rapidly elongate and form a dense cellular network within this unrestrictive environment. Subsequent contraction of the whole system rendered a smaller and stronger final tissue-like construct. This system was considered biomimetic as it promotes high cell-cell interaction and cellular condensation, which are events that occur in bone and cartilage development. This system was extensively characterized with osteoprogenitor MC3T3-E1 cells that could undergo full osteogenic differentiation under chemical induction. More interestingly, the three-dimensional microenvironment was also able to promote by itself spontaneous expression of bone-related markers. Due to the interesting properties of this system, the same model was used to induce chondrogenic differentiation of human dermal fibroblasts. This cell type has been poorly explored for tissue engineering applications, but it might have great potential in future therapeutic platforms. This work provides proof of concept of chondrogenic potential of these cells in this three-dimensional system.
Carne, Naomi Angharad. "The effect of endoplasmic reticulum and reductive stress on the human dermal fibroblast proteome". Thesis, Durham University, 2018. http://etheses.dur.ac.uk/12794/.
Texto completoWang, Yongliang. "Human dermal fibroblast activation under pulsed electrical stimulation via conductive fabrics : signalling pathways and potential benefit for wound healing". Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26437.
Texto completoDuring skin wound healing, cutaneous cells particularly fibroblasts and keratinocytes as well as several growth factors play important roles. Wound healing can be activated by exogenous factors, including electrical stimulation (ES). ES can also modulate fibroblast functions. Fibroblasts contribute to healing by secreting structural proteins (collagen, fibronectin, elastin) to repair the wound area. Fibroblasts also adopt a contractile phenotype expressing α-actin contributing to wound closure. The hypothesis of the thesis is that fibroblasts proliferate and transdifferentiate into myofibroblasts by sensing pulsed electrical signals and adjusting relevant signalling pathways. To test this hypothesis we used biocompatible polyethylene terephthalate (PET) fabrics coated with electrically conductive polypyrrole (PPy). Human dermal fibroblasts were cultured on these conductive fabrics and exposed to the optimized pulsed ES: either 10s PES in a period of 1200s, or 300s PES in 600s period, for a total of 24 hours. Two electric intensities were studied, 50 and 100 mV/ mm. Our work showed that the PES promoted the adhesion, proliferation and migration of dermal fibroblasts. These cellular activities were consolidated by an elevated level of fibroblast growth factor 2 (FGF2) and the high expression of α-smooth muscle actin (α-SMA). Important findings were that PES promoted the phenotypic change of fibroblasts to myofibroblasts, and such change was coordinated through the Smad and TGFβ/ERK pathways. It also demonstrated that the effect of PES was able to maintain for a long period of time after the end of stimulation, and was transferable from the mother cells to the daughter cells. Following subculture, the electrically stimulated fibroblasts still expressed significant amount of α-SMA. In conclusion, this thesis demonstrates that PES through conductive fabrics can activate the wound healing functions in human dermal fibroblasts. This work revealed for the first time that Smad and TGFβ/ERK pathways are required by the PES-induced fibroblasts-to-myofibroblasts differentiation. This work also demonstrated that the PES activated cells can survive in vivo. These studies suggest the application of the PES in promoting tissue regeneration and wound healing.
Groult, Vanina. "Interactions des fibroblastes de derme humain en culture avec l'élastine". Paris 12, 1992. http://www.theses.fr/1992PA120013.
Texto completoZague, Vivian. "Influência da suplementação com colágeno hidrolisado no metabolismo da matriz extracelular e proliferação de fibroblastos dérmicos humanos derivados de áreas fotoprotegida e fotoexposta, cultivados em monocamada e equivalente dérmico". Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-08122015-202409/.
Texto completoThis study investigated, for the first time, the influence of CH on the extracellular matrix metabolism and proliferation of human dermal fibroblasts (HDFs) derived from sun-protected and sun-exposed body sites, cultured in monolayer in vitro model. Moreover, CH effects on the secretion of type I collagen were investigated in dermal equivalent 3D model derived from dermal matrix produced exclusively by HDFs. CH treatment did not affect cellular proliferation of either cell cultures, but notably modulated cell metabolism in monolayer model, increasing the content of procollagen I and collagen I and decreasing metalloproteinase activity (MMP) 1 and 2. These effects were confirmed in the human dermal equivalent model. Lower concentrations of CH were enough to stimulate sun-exposed-derived HDFs, suggesting more pronounced effect in these cells. This study presents an important contribution to understanding the biological effects of CH in skin cells and viability of its use as a functional ingredient in food supplements.
El-Sheemy, Mohamed Adbo. "The effects of extracts from the human dermis on the ability of the human fibroblasts to cause contraction in vitro". Thesis, University of Aberdeen, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323397.
Texto completoTupet-Defrance, Armelle. "Modulation de la fonctionnalité des integrines par les rayonnements ultraviolets A dans les fibroblastes du derme humain". Paris 7, 1999. http://www.theses.fr/1999PA077240.
Texto completoGrandemange, Stéphanie. "Bases moléculaires de l'induction de la transformation des fibroblastes du derme humain après surexpression du récepteur mitonchondrial de la triiodothyronine". Montpellier 2, 2005. http://www.theses.fr/2005MON20086.
Texto completoRenaud-Salis, Valérie. "Alterations fonctionnelles induites par irradiation gamma a faibles doses du fibroblaste de derme humain, au cours du vieillissement in vitro (collagene, fibronectine, metalloproteinases, metalloendopeptidase)". Paris 11, 1991. http://www.theses.fr/1991PA112264.
Texto completoKiezel-Tsugunova, Magdalena. "Elucidating the metabolism of n-3 polyunsaturated fatty acids and formation of bioactive lipid mediators in human skin". Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/elucidating-the-metabolism-of-n3-polyunsaturated-fatty-acids-and-formation-of-bioactive-lipid-mediators-in-human-skin(773abedd-c726-4dab-890a-694a96b1c074).html.
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