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Xu, Cynthia M., Catherine Karbasiafshar, Rayane Brinck Teixeira, Nagib Ahsan, Giana Blume Corssac, Frank W. Sellke y M. Ruhul Abid. "Proteomic Assessment of Hypoxia-Pre-Conditioned Human Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Demonstrates Promise in the Treatment of Cardiovascular Disease". International Journal of Molecular Sciences 24, n.º 2 (14 de enero de 2023): 1674. http://dx.doi.org/10.3390/ijms24021674.
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Human bone marrow mesenchymal stem cell derived-extracellular vesicles (HBMSC-EV) are known for their regenerative and anti-inflammatory effects in animal models of myocardial ischemia. However, it is not known whether the efficacy of the EVs can be modulated by pre-conditioning of HBMSC by exposing them to either starvation or hypoxia prior to EV collection. HBMSC-EVs were isolated following normoxia starvation (NS), normoxia non-starvation (NNS), hypoxia starvation (HS), or hypoxia non-starvation (HNS) pre-conditioning. The HBMSC-EVs were characterized by nanoparticle tracking analysis, electron microscopy, Western blot, and proteomic analysis. Comparative proteomic profiling revealed that starvation pre-conditioning led to a smaller variety of proteins expressed, with the associated lesser effect of normoxia versus hypoxia pre-conditioning. In the absence of starvation, normoxia and hypoxia pre-conditioning led to disparate HBMSC-EV proteomic profiles. HNS HBMSC-EV was found to have the greatest variety of proteins overall, with 74 unique proteins, the greatest number of redox proteins, and pathway analysis suggestive of improved angiogenic properties. Future HBMSC-EV studies in the treatment of cardiovascular disease may achieve the most therapeutic benefits from hypoxia non-starved pre-conditioned HBMSC. This study was limited by the lack of functional and animal models of cardiovascular disease and transcriptomic studies.
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Cho, Hee-Yeon, Sooho Lee, Ji-Hong Park, Yoon Hae Kwak, HaeYong Kweon y Dongchul Kang. "Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts". Current Issues in Molecular Biology 44, n.º 4 (28 de marzo de 2022): 1497–512. http://dx.doi.org/10.3390/cimb44040102.
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Mesenchymal stem cells (MSCs) have been widely applied to the regeneration of damaged tissue and the modulation of immune response. The purity of MSC preparation and the delivery of MSCs to a target region are critical factors for success in therapeutic application. In order to define the molecular identity of an MSC, the gene expression pattern of a human bone marrow-derived mesenchymal stem cell (hBMSC) was compared with that of a human embryonic fibroblast (hEF) by competitive hybridization of a microarray. A total of 270 and 173 genes were two-fold up- and down-regulated with FDR < 0.05 in the hBMSC compared to the hEF, respectively. The overexpressed genes in the hBMSC over the hEF, including transcription factors, were enriched for biological processes such as axial pattern formation, face morphogenesis and skeletal system development, which could be expected from the differentiation potential of MSCs. CD70 and CD339 were identified as additional CD markers that were up-regulated in the hBMSC over the hEF. The differential expression of CD70 and CD339 might be exploited to distinguish hEF and hBMSC. CMKLR1, a chemokine receptor, was up-regulated in the hBMSC compared to the hEF. RARRES2, a CMKLR1 ligand, stimulated specific migration of the hBMSC, but not of the hEF. RARRES2 manifested as ~two-fold less effective than SDF-1α in the directional migration of the hBMSC. The expression of CMKLR1 was decreased upon the osteoblastic differentiation of the hBMSC. However, the RARRES2-loaded 10% HA-silk scaffold did not recruit endogenous cells to the scaffold in vivo. The RARRES2–CMKLR1 axis could be employed in recruiting systemically delivered or endogenous MSCs to a specific target lesion.
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Sun, Aining, Guo Feng, Jingjing Xu, Wenjuan Wang y Wu Depei. "The Involvement of NF-κB Subunits in the Interaction Between CLL-B Cells and Bone Marrow Stromal Cells". Blood 118, n.º 21 (18 de noviembre de 2011): 4590. http://dx.doi.org/10.1182/blood.v118.21.4590.4590.
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Abstract Abstract 4590 Objective: To investigate the function of NF-κB signaling pathway in the interaction between chronic lymphocytic leukemia B cells and bone marrow stromal cells. Method: The expression of NF-κB family members at mRNA and protein levels were examined by quantitative RT-PCR and Western bloting analyses individualy, and the differences between CLL-human bone marrow stromal cell (hBMSC) and non-CLL-hBMSC were investigated with the methods above. Cell death was measured by flow cytometry analysis after B-CLL cells were co-cultured with hBMSC and treated with proteasome inhibitors. The changes of NF-κB expression at the protein levels were examined by Western bloting after co-culture experiment. Result: The expression of NF-κB family members turned out to be heterogeneous at both mRNA and protein levels in B-CLL cells, and the members demonstrated a different κB-DNA binding activities. The mRNA expression of NF-κB family members in B-CLL cells was shown at a remarkable higher level than that of the controls. The relative mRNA expression of relA was 0.0214±0.012, whereas it was 0.0130±0.012 for the controls of CD19 positive cells. The relative mRNA expression of p50 and p52 were 66.0860±21.649 and 0.0208±0.011 respectively, and the corresponding expression of CD19 positive cells were 24.8440±9.749 and 0.0065±0.002. The differences were statistically significant. There was no notable difference between CLL-hBMSC and Non-CLL-hBMSC. hBMSC protected B-CLL cells against the proteasome inhibitors and facilitated the survival of B-CLL cells. Conclusion: NF-κB expression is heterogeneous in bone marrow B-CLL cells. There is no significant difference between CLL-hBMSC and non-CLL-hBMSC. hBMSC can protect the survival of B-CLL cells dependending on the endogenous NF-κB activity. hBMSC can increase the drug resistance of B-CLL cells to proteasome inhibitors. Disclosures: No relevant conflicts of interest to declare.
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Yang, Hongwei, Meng Cong, Weixiao Huang, Jin Chen, Min Zhang, Xiaosong Gu, Cheng Sun y Huilin Yang. "The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in Rabbits". Stem Cells International 2022 (8 de septiembre de 2022): 1–12. http://dx.doi.org/10.1155/2022/5760107.
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Mesenchymal stem cells (MSCs) have shown chondroprotective effects in cartilage repair. However, side effects caused by MSC treatment limit their application in clinic. As a cell-free therapy, MSC-derived exosomes (EXOs) have attracted much more attention in recent years. In the present study, we prepared EXOs from human bone marrow mesenchymal stem cells (hBMSCs) and examined their therapeutic potentials in cartilage repair. Our results showed that the prepared extracellular vesicles exhibit classical features of EXOs, such as cup-like shape, around 100 nm diameter, positive protein markers (CD81, TSG101, and Flotillin 1), and ability of internalization. In primary chondrocytes, the treatment of hBMSC-EXOs markedly increases cell viability and proliferation in a dose-dependent manner. Moreover, wound healing assay showed that hBMSC-EXOs accelerate cell migration in primary chondrocytes. JC-1 staining revealed that the mitochondrial membrane potential was enhanced by hBMSC-EXOs, indicating cell apoptosis was decreased in the presence of hBMSC-EXOs. In rabbits with articular cartilage defects, local administration with hBMSC-EXOs facilitates cartilage regeneration as evidenced by gross view and hematoxylin-eosin (H&E) and Saf-O/Fast Green staining. In addition, the International Cartilage Repair Society (ICRS) score was increased by the application of hBMSC-EXOs. Overall, our data indicate that the treatment with hBMSC-EXOs is a suitable cell-free therapy for treating cartilage defects, and these benefits are likely due to improved cell proliferation and migration in chondrocytes.
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Chikato, Masao Koda, Koshiro Kamiya, Mari Dezawa, Masayuki Hashimoto, Takeo Furuya, Akihiko Okawa, Kazuhisa Takahashi y Masashi Yamazaki. "Transplantation of human bone marrow stromal cell-derived neuroregenrative cells promotes functional recovery after spinal cord injury in mice". Acta Neurobiologiae Experimentalis 74, n.º 4 (31 de diciembre de 2014): 479–88. http://dx.doi.org/10.55782/ane-2014-2010.
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Transplantation of bone marrow stromal cells (BMSCs) for spinal cord injury (SCI) has been shown to improve functional outcome. BMSCs can be easily obtained from bone marrow aspirate and have fewer problems in the clinical application for human SCI from the ethical and legal points of view. Recently, we produced cells with neural stem and/or progenitor cell property and neural regeneration supporting capacity from human bone marrow stromal cells (human bone marrow stroma cell-derived neuroregenerative cells: hBMSC-NRs). The aim of the present study was to clarify the effectiveness of transplantation of hBMSC-NRs to injured spinal cord of severe combined immunodeficiency (NOD/SCID) mice. Neurite outgrowth assay of PC-12 cells was performed. One week after a T9-level contusion SCI, hBMSCs or hBMSC-NRs were transplanted into the spinal cord. After the transplantation, functional and histological examinations were performed. Conditioned media of hBMSC-NRs significantly promoted the neurite outgrowth of PC-12 cells in vitro. Transplanted hBMSC-NRs survived in the injured spinal cord 8 weeks after SCI. Immunohistochemistry revealed that the density of serotonin-positive fibers of the transplanted group was significantly higher than that of the control group at the epicenter and caudal segment to the injured site. The recovery of hind limb function of the hBMSC-NRs group was significantly better than that of the control group. In conclusion, hBMSC-NRs can be one of the realistic candidates for cell transplantation therapy for human SCI.
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Tam, Kin-Wai, Cheuk-Yin Wong, Kenneth Lap-Kei Wu, Guy Lam, Xiaotong Liang, Wai-Ting Wong, Maximilian Tak-Sui Li et al. "IPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries". Cells 12, n.º 11 (25 de mayo de 2023): 1479. http://dx.doi.org/10.3390/cells12111479.
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The in vitro derivation of Schwann cells from human bone marrow stromal cells (hBMSCs) opens avenues for autologous transplantation to achieve remyelination therapy for post-traumatic neural regeneration. Towards this end, we exploited human induced pluripotent stem-cell-derived sensory neurons to direct Schwann-cell-like cells derived from among the hBMSC-neurosphere cells into lineage-committed Schwann cells (hBMSC-dSCs). These cells were seeded into synthetic conduits for bridging critical gaps in a rat model of sciatic nerve injury. With improvement in gait by 12-week post-bridging, evoked signals were also detectable across the bridged nerve. Confocal microscopy revealed axially aligned axons in association with MBP-positive myelin layers across the bridge in contrast to null in non-seeded controls. Myelinating hBMSC-dSCs within the conduit were positive for both MBP and human nucleus marker HuN. We then implanted hBMSC-dSCs into the contused thoracic cord of rats. By 12-week post-implantation, significant improvement in hindlimb motor function was detectable if chondroitinase ABC was co-delivered to the injured site; such cord segments showed axons myelinated by hBMSC-dSCs. Results support translation into a protocol by which lineage-committed hBMSC-dSCs become available for motor function recovery after traumatic injury to both peripheral and central nervous systems.
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Ramalingam, Mahesh, Sujeong Jang y Han-Seong Jeong. "Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis". Stem Cells International 2021 (22 de enero de 2021): 1–16. http://dx.doi.org/10.1155/2021/6658271.
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Mesenchymal stem cells (MSCs) have been used against several diseases. Their potential mainly appears from its secreted biomolecules. Human bone marrow-derived stem cells (hBMSC) displayed neuronal functional characteristics after differentiation by basic fibroblast growth factor (bFGF) and forskolin. PD is a chronic age-related neurodegenerative disease (NDD) characterized by loss of dopaminergic neurons in the substantia nigra (SN) and abnormal accumulation of α-synuclein (α-syn) aggregations. In this present study, we evaluated the therapeutic effects of neural differentiated hBMSC (NI-hBMSC) conditioned medium (NI-hBMSC-CM) to a rotenone- (ROT-) induced Parkinson’s disease (PD) model in SH-SY5Y cells. NI-hBMSC-CM treatment (50% diluted) in the last 24 h of 48 h ROT (0.5 μM) toxicity showed a significant increase in cell survival. The decreased tyrosine hydroxylase (TH) expression as a hallmark of PD was increased by NI-hBMSC-CM. The Triton X-100-soluble and Triton X-100-insoluble cell lysate fractions were used in Western blotting. The oligomeric, dimeric, and monomeric phosphorylated serine129 (p-S129) α-syn and total monomeric α-syn were decreased during ROT toxicity in the Triton X-100-soluble fraction. The Triton X-100-insoluble fraction revealed that ROT toxicity significantly increased the oligomeric but decreased the dimeric and monomeric p-S129 α-syn expressions while all forms of total α-syn were increased in SH-SY5Y cells. NI-hBMSC-CM stabilized the physiological α-syn monomers and reduced aggregated insoluble p-S129 α-syn against ROT. The cytoskeletal proteins, neurofilament-H (NF-H), β3-tubulin (Tuj1), neuronal nuclei (NeuN), and synaptophysin (SYP) were significantly decreased during ROT toxicity. In addition, proapoptotic Bax was increased by ROT with decreased antiapoptotic Bcl-2 and Mcl-1 as well as proforms of caspase-9, caspase-3, caspase-7, and PARP-1. NI-hBMSC-CM ameliorated the neurotrophic protein expressions, controlled the Bax/Bcl-2 ratio, upregulated procaspases, and inactivated PARP-1. From our results, we conclude that NI-hBMSC-CM containing released biomolecules during neural differentiation employs regenerative effects on the ROT model of PD in SH-SY5Y cells.
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Wang, Baodong, Na Xu, Li Cao, Xiaojun Yu, Shanxi Wang, Qikun Liu, Yinguang Wang, Haoran Xu y Yang Cao. "miR-31 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviates Intervertebral Disc Degeneration by Inhibiting NFAT5 and Upregulating the Wnt/β-Catenin Pathway". Stem Cells International 2022 (20 de octubre de 2022): 1–16. http://dx.doi.org/10.1155/2022/2164057.
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In this study, we explored the regulatory mechanism of intervertebral disc degeneration (IDD) that involves miR-31 shuttled by bone marrow mesenchymal stem cell-derived extracellular vesicles (BMSC-EVs) and its downstream signaling molecules. Nucleus pulposus cells (NPCs) were isolated and treated with TNF-α to simulate IDD in vitro. The TNF-α-exposed NPCs were then cocultured with hBMSCs or hBMSC-EVs in vitro to detect the effects of hBMSC-EVs on NPC viability, apoptosis, and ECM degradation. Binding between miR-31 and NFAT5 was determined. A mouse model of IDD was prepared by vertebral disc puncture and injected with EVs from hBMSCs with miR-31 knockdown to discern the function of miR-31 in vivo. The results demonstrated that hBMSC-EVs delivered miR-31 into NPCs. hBMSC-EVs enhanced NPC proliferation and suppressed cell apoptosis and ECM degradation, which was associated with the transfer of miR-31 into NPCs. In NPCs, miR-31 bound to the 3 ′ UTR of NFAT5 and inhibited NFAT5 expression, leading to activation of the Wnt/β-catenin pathway and thus promoting NPC proliferation and reducing cell apoptosis and ECM degradation. In addition, miR-31 in hBMSC-EVs alleviated the IDD in mouse models. Taken together, miR-31 in hBMSC-EVs can alleviate IDD by targeting NFAT5 and activating the Wnt/β-catenin pathway.
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Zhang, Zhen, Yi Liu, Xuelian Tao, Ping Du, Myagmartsend Enkhbat, Khoon S. Lim, Huaiyu Wang y Peng-Yuan Wang. "Engineering Cell Microenvironment Using Nanopattern-Derived Multicellular Spheroids and Photo-Crosslinked Gelatin/Hyaluronan Hydrogels". Polymers 15, n.º 8 (18 de abril de 2023): 1925. http://dx.doi.org/10.3390/polym15081925.
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Cell cultures of dispersed cells within hydrogels depict the interaction of the cell–extracellular matrix (ECM) in 3D, while the coculture of different cells within spheroids combines both the effects of cell–cell and cell–ECM interactions. In this study, the cell co-spheroids of human bone mesenchymal stem cells/human umbilical vein endothelial cells (HBMSC/HUVECs) are prepared with the assistance of a nanopattern, named colloidal self-assembled patterns (cSAPs), which is superior to low-adhesion surfaces. A phenol-modified gelatin/hyaluronan (Gel-Ph/HA-Ph) hydrogel is used to encapsulate the multicellular spheroids and the constructs are photo-crosslinked using blue light. The results show that Gel-Ph/HA-Ph hydrogels with a 5%-to-0.3% ratio have the best properties. Cells in HBMSC/HUVEC co-spheroids are more favorable for osteogenic differentiation (Runx2, ALP, Col1a1 and OPN) and vascular network formation (CD31+ cells) compared to HBMSC spheroids. In a subcutaneous nude mouse model, the HBMSC/HUVEC co-spheroids showed better performance than HBMSC spheroids in angiogenesis and the development of blood vessels. Overall, this study paves a new way for using nanopatterns, cell coculturing and hydrogel technology for the generation and application of multicellular spheroids.
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Choi, Andrew, Hyungjun Yoon, Seon Jin Han, Ji-Ho Lee, In Hyeok Rhyou y Dong Sung Kim. "Rapid harvesting of stem cell sheets by thermoresponsive bulk poly(N-isopropylacrylamide) (PNIPAAm) nanotopography". Biomaterials Science 8, n.º 19 (2020): 5260–70. http://dx.doi.org/10.1039/d0bm01338b.
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A novel concept of utilizing bulk PNIPAAm substrate nanotopography for rapid harvest of human bone marrow mesenchymal stem cells (hBMSCs) sheet. The created nanotogporaphy significantly accelerated the formation of hBMSC layers and eased the detachment of hBMSC sheets.
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Li, Yanghui, Tingting Luo, Xiang Sun, Weiguo Liu, Qingping Li, Yuanping Li y Yongchen Song. "Strength Behaviors of Remolded Hydrate-Bearing Marine Sediments in Different Drilling Depths of the South China Sea". Energies 12, n.º 2 (15 de enero de 2019): 253. http://dx.doi.org/10.3390/en12020253.
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The mechanical behaviors of hydrate-bearing marine sediments (HBMS) drilled from the seafloor need to be understood in order to safely exploit natural gas from marine hydrate reservoirs. In this study, hydrates were prepared using ice powder and CH4 gas, and HBMS from the Shenhu area in the South China Sea were remolded using a mixed sample preparation method. A series of triaxial tests were conducted on the remolded HBMS to investigate the effects of soil particle gradation and the existence of hydrate on the mechanical properties of hydrate reservoirs. The results show that the stiffness and failure strength of HBMS decrease along with the decrease of mean particle size and soil aggregate morphology change at different drilling depths, and the reduction of failure strength is more than 20% when the drilling depth drops by 30 m. A better particle gradation of marine sediments may boost the stiffness and failure strength of HBMS. In addition, the existence of hydrate plays an important role in the strength behaviors of HBMS. The reduction of failure strength of HBMS with 30% initial hydrate saturation is more than 35% after complete hydrate dissociation.
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Rochira, Alessio, Luisa Siculella, Fabrizio Damiano, Andrea Palermo, Franco Ferrante, Maria Annunziata Carluccio, Nadia Calabriso, Laura Giannotti y Eleonora Stanca. "Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells". Biology 9, n.º 11 (30 de octubre de 2020): 370. http://dx.doi.org/10.3390/biology9110370.
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Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field.
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Villars, F., B. Guillotin, T. Amédée, S. Dutoya, L. Bordenave, R. Bareille y J. Amédée. "Effect of HUVEC on human osteoprogenitor cell differentiation needs heterotypic gap junction communication". American Journal of Physiology-Cell Physiology 282, n.º 4 (1 de abril de 2002): C775—C785. http://dx.doi.org/10.1152/ajpcell.00310.2001.
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Bone development and remodeling depend on complex interactions between bone-forming osteoblasts and other cells present within the bone microenvironment, particularly vascular endothelial cells that may be pivotal members of a complex interactive communication network in bone. Our aim was to investigate the interaction between human umbilical vein endothelial cells (HUVEC) and human bone marrow stromal cells (HBMSC). Cell differentiation analysis performed with different cell culture models revealed that alkaline phosphatase activity and type I collagen synthesis were increased only by the direct contact of HUVEC with HBMSC. This “juxtacrine signaling” could involve a number of different heterotypic connexions that require adhesion molecules or gap junctions. A dye coupling assay with Lucifer yellow demonstrated a functional coupling between HUVEC and HBMSC. Immunocytochemistry revealed that connexin43 (Cx43), a specific gap junction protein, is expressed not only in HBMSC but also in the endothelial cell network and that these two cell types can communicate via a gap junctional channel constituted at least by Cx43. Moreover, functional inhibition of the gap junction by 18α-glycyrrhetinic acid treatment or inhibition of Cx43 synthesis with oligodeoxyribonucleotide antisense decreased the effect of HUVEC cocultures on HBMSC differentiation. This stimulation could be mediated by the intercellular diffusion of signaling molecules that permeate the junctional channel.
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Hempel, Ute, Katrin Müller, Carolin Preissler, Carolin Noack, Sabine Boxberger, Peter Dieter, Martin Bornhäuser y Manja Wobus. "Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool forIn VitroOsteogenesis and Bone Tissue Engineering Approaches". Stem Cells International 2016 (2016): 1–14. http://dx.doi.org/10.1155/2016/7842191.
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Adult human bone marrow stromal cells (hBMSC) are important for many scientific purposes because of their multipotency, availability, and relatively easy handling. They are frequently used to study osteogenesisin vitro. Most commonly, hBMSC are isolated from bone marrow aspirates collected in clinical routine and cultured under the “aspect plastic adherence” without any further selection. Owing to the random donor population, they show a broad heterogeneity. Here, the osteogenic differentiation potential of 531 hBMSC was analyzed. The data were supplied to correlation analysis involving donor age, gender, and body mass index. hBMSC preparations were characterized as follows: (a) how many passages the osteogenic characteristics are stable in and (b) the influence of supplements and culture duration on osteogenic parameters (tissue nonspecific alkaline phosphatase (TNAP), octamer binding transcription factor 4, core-binding factor alpha-1, parathyroid hormone receptor, bone gla protein, and peroxisome proliferator-activated proteinγ). The results show that no strong prediction could be made from donor data to the osteogenic differentiation potential; only the ratio of induced TNAP to endogenous TNAP could be a reliable criterion. The results give evidence that hBMSC cultures are stable until passage 7 without substantial loss of differentiation potential and that established differentiation protocols lead to osteoblast-like cells but not to fully authentic osteoblasts.
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Krysa, B. D. "Zinc pressure leaching at HBMS". Hydrometallurgy 39, n.º 1-3 (octubre de 1995): 71–77. http://dx.doi.org/10.1016/0304-386x(95)00046-j.
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Stewart, K. B., A. M. Adams, S. L. Pratt y S. L. Stice. "193CHARACTERIZATION OF PORCINE TROPHECTODERM-DERIVED CELLS IN THE PRESENCE OF HBMP4 IN THE ABSENCE OF A FEEDER LAYER". Reproduction, Fertility and Development 16, n.º 2 (2004): 218. http://dx.doi.org/10.1071/rdv16n1ab193.
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A porcine trophoblastic cell line could provide a powerful model for understanding trophoblast cell biology as well as placental gene expression and proteomics in vitro. In this experiment, we derived porcine trophoblastic cells from trophectoderm tissue and assessed their growth on three different extracellular matrix substrates and in three different concentrations of human recombinant bone morphogenetic protein 4 (hBMP4). Human BMP4 has been shown to induce differentiation of human embryonic stem cells into trophoblast lineages. Elongated embryos were flushed using DPBS supplemented with 1% fetal calf serum and penicillin-streptomycin (1X) from the hysterectomized uteri of superovulated and bred prepuberal gilts 15 days post-insemination. The embryonic disc was visualized with a dissecting microscope. The trophectoderm tissue was cut 2–3mm away from the embryonic disc with a scalpel and the trophectoderm tissue was manually dissected into cell aggregates. These aggregates were plated on collagen type IV, Matrigel, and human extracellular matrix (laminin, collagen type IV and heparan sulfate proteoglycan derived from human placenta) in culture medium (DMEM with 15% FCS, 0.1mM 2-mercaptoethanol, 4ngmL−1 basic FGF4 and 1X P/S) in the presence or absence of hBMP4 at 0, 10, or 20ngmL−1. Cell outgrowth was observed within 24 hours of culture. After three days of culture, various cell types (based on size and morphology) were present. Among cultures of predominant large cells were colonies of smaller cells with epithelial-type morphology that had a prominent nucleus and a high nuclear-to-cytoplasmic ratio. The epithelial-type cells grew in tight colonies with definite borders and contained cytoplasmic structures resembling lipid-containing vesicles. These colonies initially appeared on all matrices across all hBMP4 concentrations. After seven days in culture the colonies developed distinct differences across groups. Cell growth on collagen was comprised of tight colonies having definite borders among large cells. Colonies on collagen were larger and more pronounced in both the hBMP4-supplemented groups than when cultured without hBMP4. The Matrigel coated plates contained large sheets of epithelial-type cell growth instead of compact colonies. This type of growth characteristic was present in all hBMP4 treatments on Matrigel. In contrast, few cells survived and propagated on human extracellular matrix. Only small colonies having the desired morphology were among the large cells on human extracellular matrix when cultured in medium containing 10ngmL−1 hBMP4. Cells were passaged and only cells growing on Matrigel could be further cultured. These data suggest that both the cell substrate and hBMP4 affect initial trophoblast outgrowths. Further analysis including immunocytochemistry and RT-PCR is currently being performed to better characterize these cells. Epidemiology/Diseases
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Wu, Gang, Ying Jun Wang y Kun Wei. "Behavior of hBMSC on Nano Hydroxyapatite Surface". Advanced Materials Research 105-106 (abril de 2010): 542–45. http://dx.doi.org/10.4028/www.scientific.net/amr.105-106.542.
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The bioactivity of HA has been tested by its osteoconduction ability in vivo. Nano HA has higher specific surface area and was presumed to have high bioactivity. In this paper, nano needle HA was synthesized by the microemulsion method. The properties of such nano materials were analyzed by the XRD and TEM. The impact of the nano HA on the expression of two hBMSCs’ genes, inhibitor differentiation 2 and ostopontin, and the ALP activity was researched in this paper.
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Jung, I., E. Kim, J. Moon, S. Kang y J. Chang. "P14.42 Diagnostic challenges of infratentorial hemangioblastomas: improvement of current radiological classification scheme". Neuro-Oncology 21, Supplement_3 (agosto de 2019): iii76. http://dx.doi.org/10.1093/neuonc/noz126.277.
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Abstract BACKGROUND Hemangioblastomas (HBMs) are known to exhibit very typical radiological features and thus classified by well-established radiological classification scheme. However, after we have experienced unusual cases in which current radiological classification system was not enough to categorize them, we reviewed our series of infratentorial HBMs in order not only to evaluate the relevance of current classification scheme, but also to possibly improve it. Also, we added descriptions on several cases with unusual radiological magnetic resonance imaging (MRI) findings in which differential diagnosis was challenging. MATERIAL AND METHODS We retrospectively reviewed preoperative MRI of 118 patients with pathologically diagnosed infratentorial HBMs at our institution between 2002 and 2015. Total 128 tumors were included to this study and classified into four categories based on the presence and nature of cystic components: extratumoral cystic (Type Ce, classical cystic with a mural nodule), intratumoral cystic (Type Ci), mixed cystic (Type Cm), and solid (Type S). The association with von Hippel-Lindau (VHL) disease was also investigated. RESULTS In 118 patients (65 male and 53 female), 79 (66.9%) had solitary HBMs and 39 (33.1%) were diagnosed with VHL disease. Type Ce with typical radiological findings was the most prevalent type of HBM (63.3%), followed by Type S (21.1%). HBMs with intratumoral cysts were uncommon (Type Ci, 11.7%) and mixed extratumoral and intratumoral cysts (Type Cm) accounted for only 3.9%. No intergroup differences were observed in the proportions of each subtype between the solitary and VHL disease-associated HBMs. CONCLUSION Radiological features of HBMs are usually typical thus preoperative presumption is not difficult in majority of cases. Improved radiological classification scheme is more practical because it does not only help surgeons determine whether the cystic wall should be removed or not, but also covers cases with atypical radiological presentations. For solid and extraparenchymal HBMs, differential diagnosis is more difficult as well as very critical as surgical removal is often very challenging.
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Hutapea, Nahor Murani. "IMPLEMENTASI PENDEKATAN KONTEKSTUAL UNTUK MENINGKATKAN HASIL BELAJAR MATEMATIKA SISWA". Jurnal Prinsip Pendidikan Matematika 1, n.º 1 (27 de noviembre de 2018): 39–46. http://dx.doi.org/10.33578/prinsip.v1i1.20.
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Berdasarkan hasil observasi di lapangan, hasil belajar matematika siswa (HBMS) masih rendah. Hal ini dikarenakan kegiatan pembelajaran masih didominasi oleh guru; siswa kurang berperan aktif dalam proses pembelajaran (baik individu atau kelompok); dan siswa kurang diberikan kesempatan untuk menghubungkan apa yang telah dipelajari dengan dunia nyata yang dialami oleh siswa, sehingga proses belajar kurang bermakna. Untuk itu telah dilakukan penelitian tindakan dengan model desain siklus, melalui implementasi pendekatan kontekstual yang bertujuan untuk memperbaiki proses pembelajaran dan meningkatkan HBMS. Penelitian dilakukan di SMP Muhammadiyah 1 Pekanbaru, subjek dalam penelitian ini adalah siswa kelas VII-4 yang terdiri dari 25 siswa dengan tingkat kemampuan heterogen. Instrumen penelitian in adalah perangkat tes HBMS, lembar observasi, dan perangkat pembelajaran. Perangkat pembelajaran terdiri dari rencana pelajaran dan lembar kerja siswa. Data aktivitas dianalisis dengan statistik narasi, HBMS dianalisis dengan statistik deskriptif. Hasil pengamatan kegiatan (guru, siswa) terjadi perbaikan proses pembelajaran setelah tindakan pada siklus pertama dan kedua dan terjadi peningkatan jumlah persentase siswa dari skor dasar ke tes matematika 1 dan dari tes matematika 1 ke tes matematika 2. Kesimpulan dari penelitian ini adalah implementasi pendekatan kontekstual dapat memperbaiki proses pembelajaran dan dapat meningkatkan HBMS di topik himpunan.
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Man, Kenny, Mathieu Y. Brunet, Rebecca Lees, Ben Peacock y Sophie C. Cox. "Epigenetic Reprogramming via Synergistic Hypomethylation and Hypoxia Enhances the Therapeutic Efficacy of Mesenchymal Stem Cell Extracellular Vesicles for Bone Repair". International Journal of Molecular Sciences 24, n.º 8 (20 de abril de 2023): 7564. http://dx.doi.org/10.3390/ijms24087564.
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Mesenchymal stem cells (MSCs) are a promising cell population for regenerative medicine applications, where paracrine signalling through the extracellular vesicles (EVs) regulates bone tissue homeostasis and development. MSCs are known to reside in low oxygen tension, which promotes osteogenic differentiation via hypoxia-inducible factor-1α activation. Epigenetic reprogramming has emerged as a promising bioengineering strategy to enhance MSC differentiation. Particularly, the process of hypomethylation may enhance osteogenesis through gene activation. Therefore, this study aimed to investigate the synergistic effects of inducing hypomethylation and hypoxia on improving the therapeutic efficacy of EVs derived from human bone marrow MSCs (hBMSCs). The effects of the hypoxia mimetic agent deferoxamine (DFO) and the DNA methyltransferase inhibitor 5-azacytidine (AZT) on hBMSC viability was assessed by quantifying the DNA content. The epigenetic functionality was evaluated by assessing histone acetylation and histone methylation. hBMSC mineralisation was determined by quantifying alkaline phosphate activity, collagen production and calcium deposition. EVs were procured from AZT, DFO or AZT/DFO-treated hBMSCs over a two-week period, with EV size and concentration defined using transmission electron microscopy, nanoflow cytometry and dynamic light scattering. The effects of AZT-EVs, DFO-EVs or AZT/DFO-EVs on the epigenetic functionality and mineralisation of hBMSCs were evaluated. Moreover, the effects of hBMSC-EVs on human umbilical cord vein endothelial cells (HUVECs) angiogenesis was assessed by quantifying pro-angiogenic cytokine release. DFO and AZT caused a time–dose dependent reduction in hBMSC viability. Pre-treatment with AZT, DFO or AZT/DFO augmented the epigenetic functionality of the MSCs through increases in histone acetylation and hypomethylation. AZT, DFO and AZT/DFO pre-treatment significantly enhanced extracellular matrix collagen production and mineralisation in hBMSCs. EVs derived from AZT/DFO-preconditioned hBMSCs (AZT/DFO-EVs) enhanced the hBMSC proliferation, histone acetylation and hypomethylation when compared to EVs derived from AZT-treated, DFO-treated and untreated hBMSCs. Importantly, AZT/DFO-EVs significantly increased osteogenic differentiation and mineralisation of a secondary hBMSC population. Furthermore, AZT/DFO-EVs enhanced the pro-angiogenic cytokine release of HUVECs. Taken together, our findings demonstrate the considerable utility of synergistically inducing hypomethylation and hypoxia to improve the therapeutic efficacy of the MSC-EVs as a cell-free approach for bone regeneration.
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Elsafadi, Mona, Muthurangan Manikandan, Muhammad Atteya, Jamil Amjad Hashmi, Zafar Iqbal, Abdullah Aldahmash, Musaad Alfayez, Moustapha Kassem y Amer Mahmood. "Characterization of Cellular and Molecular Heterogeneity of Bone Marrow Stromal Cells". Stem Cells International 2016 (2016): 1–18. http://dx.doi.org/10.1155/2016/9378081.
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Human bone marrow-derived stromal stem cells (hBMSC) exhibit multiple functions, including differentiation into skeletal cells (progenitor function), hematopoiesis support, and immune regulation (nonprogenitor function). We have previously demonstrated the presence of morphological and functional heterogeneity of hBMSC cultures. In the present study, we characterized in detail two hTERT-BMSC clonal cell populations termed here CL1 and CL2 that represent an opposing phenotype with respect to morphology, markers expression: alkaline phosphatase (ALP) and CD146, andex vivodifferentiation potential. CL1 differentiated readily to osteoblasts, adipocytes, and chondrocytes as shown by expression of lineage specific genes and proteins. Whole genome transcriptome profiling of CL1 versus CL2 revealed enrichment in CL1 of bone-, mineralization-, and skeletal muscle-related genes, for example,ALP,POSTN,IGFBP5 BMP4, andCXCL12. On the other hand, CL2 transcriptome was enriched in immune modulatory genes, for example,CD14, CD99, NOTCH3, CXCL6, CFB, andCFI. Furthermore, gene expression microarray analysis of osteoblast differentiated CL1 versus CL2 showed significant upregulation in CL1 of bone development and osteoblast differentiation genes which included several homeobox genes:TBX15, HOXA2andHOXA10, andIGF1, FGFR3, BMP6, MCAM, ITGA10, IGFBP5, andALP. siRNA-based downregulation of theALPgene in CL1 impaired osteoblastic and adipocytic differentiation. Our studies demonstrate the existence of molecular and functional heterogeneity in cultured hBMSC. ALP can be employed to identify osteoblastic and adipocytic progenitor cells in the heterogeneous hBMSC cultures.
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Nguyen, Alexander K., Peter L. Goering, John A. Olenick, Kathy Olenick y Roger J. Narayan. "Sintered Tape-cast 3YSZ Supports Human Bone Marrow Derived Stem Cell Osteogenic Differentiation". MRS Advances 4, n.º 46-47 (2019): 2541–49. http://dx.doi.org/10.1557/adv.2019.335.
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ABSTRACTSintered tape-cast yttria-stabilized zirconia (YSZ) was evaluated for its elemental composition, crystal structure, and imaged with atomic force microscopy (AFM) and scanning electron microscopy (SEM). Human bone marrow stem cells (hBMSC) were cultured on the ceramic and differentiated into the osteoblast lineage; alkaline phosphatase (ALP) activity was tracked as a differentiation marker. The YSZ was composed of purely tetragonal grains with a median equivalent circular diameter of 283 nm. Zirconium, yttrium, oxygen, and adventitious carbon was detected on the substrate with no other elements in significant quantities detected. YSZ samples had an RMS roughness value of 27 nm, elastic modulus of 206 ± 14 GPa, and hardness of 14 ± 2 GPa. hBMSC were observed to attach and proliferate on the YSZ surfaces and had significantly increased ALP versus the undifferentiated control cultured on glass. This method for producing a YSZ ceramic yields a typical material of this type and supports attachment and differentiation of hBMSC; thus, making it useful as a bone implant material.
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Saleem, Ragda, Samih Mohamed-Ahmed, Rammah Elnour, Ellen Berggreen, Kamal Mustafa y Niyaz Al-Sharabi. "Conditioned Medium from Bone Marrow Mesenchymal Stem Cells Restored Oxidative Stress-Related Impaired Osteogenic Differentiation". International Journal of Molecular Sciences 22, n.º 24 (15 de diciembre de 2021): 13458. http://dx.doi.org/10.3390/ijms222413458.
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Oxidative stress from high levels of intracellular reactive oxygen species (ROS) has been linked to various bone diseases. Previous studies indicate that mesenchymal stem cells (MSC) secrete bioactive factors (conditioned medium (MSC-CM)) that have antioxidant effects. However, the antioxidant role of MSC-CM on osteogenesis has not been fully studied. We aimed to identify antioxidant proteins in MSC-CM using mass spectrometry-based proteomics and to explore their effects on osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSC) exposed to oxidative stress induced by hydrogen peroxide (H2O2). Our analysis revealed that MSC-CM is comprised of antioxidant proteins that are involved in several biological processes, including negative regulation of apoptosis and positive regulation of cell proliferation. Then, hBMSC exposed to H2O2 were treated with MSC-CM, and the effects on their osteogenic differentiation were evaluated. MSC-CM restored H2O2-induced damage to hBMSC by increasing the antioxidant enzyme-SOD production and the mRNA expression level of the anti-apoptotic BCL-2. A decrease in ROS production and cellular apoptosis was also shown. MSC-CM also modulated mRNA expression levels of osteogenesis-related genes, runt-related transcription factor 2, collagen type I, bone morphogenic protein 2, and osteopontin. Furthermore, collagen type I protein secretion, alkaline phosphatase activity, and in vitro mineralization were increased. These results indicate that MSC-CM contains several proteins with antioxidant and anti-apoptotic properties that restored the impaired hBMSC osteogenic differentiation associated with oxidative stress.
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Testoni, Federico E., Mercedes García Carrillo, Marc-André Gagnon, Cecilia Rikap y Matías Blaustein. "Whose shoulders is health research standing on? Determining the key actors and contents of the prevailing biomedical research agenda". PLOS ONE 16, n.º 4 (7 de abril de 2021): e0249661. http://dx.doi.org/10.1371/journal.pone.0249661.
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Background Conflicts of interest in biomedical research can influence research results and drive research agendas away from public health priorities. Previous agenda-setting studies share two shortfalls: they only account for direct connections between academic institutions and firms, as well as potential bias based on researchers’ personal beliefs. This paper’s goal is to determine the key actors and contents of the prevailing health and biomedical sciences (HBMS) research agenda, overcoming these shortfalls. Methods We performed a bibliometric and lexical analysis of 95,415 scientific articles published between 1999 and 2018 in the highest impact factor journals within HBMS, using the Web of Science database and the CorText platform. HBMS’s prevailing knowledge network of institutions was proxied with network maps where nodes represent affiliations and edges the most frequent co-authorships. The content of the prevailing HBMS research agenda was depicted through network maps of prevalent multi-terms found in titles, keywords, and abstracts. Results The HBMS research agendas of large private firms and leading academic institutions are intertwined. The prevailing HBMS agenda is mostly based on molecular biology (40% of the most frequent multi-terms), with an inclination towards cancer and cardiovascular research (15 and 8% of the most frequent multi-terms, respectively). Studies on pathogens and biological vectors related to recent epidemics are marginal (1% of the most frequent multi-terms). Content of the prevailing HBMS research agenda prioritizes research on pharmacological intervention over research on socio-environmental factors influencing disease onset or progression and overlooks, among others, the study of infectious diseases. Conclusions Pharmaceutical corporations contribute to set HBMS’s prevailing research agenda, which is mainly focused on a few diseases and research topics. A more balanced research agenda, together with epistemological approaches that consider socio-environmental factors associated with disease spreading, could contribute to being better prepared to prevent and treat more diverse pathologies and to improve overall health outcomes.
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Sun, Yi, Yuke Tian, Haifeng Li, Dengwen Zhang y Qiang Sun. "Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain". Pain Research and Management 2017 (2017): 1–11. http://dx.doi.org/10.1155/2017/7346103.
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Background. This study aimed to investigate the use of human bone marrow mesenchymal stem cells (hBMSCs) genetically engineered with the human proenkephalin (hPPE) gene to treat bone cancer pain (BCP) in a rat model.Methods. Primary cultured hBMSCs were passaged and modified with hPPE, and the cell suspensions (6 × 106) were then intrathecally injected into a rat model of BCP. Paw mechanical withdrawal threshold (PMWT) was measured before and after BCP. The effects of hPPE gene transfer on hBMSC bioactivity were analyzed in vitro and in vivo.Results. No changes were observed in the surface phenotypes and differentiation of hBMSCs after gene transfer. The hPPE-hBMSC group showed improved PMWT values on the ipsilateral side of rats with BCP from day 12 postoperatively, and the analgesic effect was reversed by naloxone. The levels of proinflammatory cytokines such as IL-1βand IL-6 were ameliorated, and leucine-enkephalin (L-EK) secretion was augmented, in the hPPE-engineered hBMSC group.Conclusion. The intrathecal administration of BMSCs modified with the hPPE gene can effectively relieve pain caused by bone cancer in rats and might be a potentially therapeutic tool for cancer-related pain in humans.
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Zhang, Xiangkai, Yanlun Zhu, Lingyan Cao, Xiao Wang, Ao Zheng, Jiang Chang, Jiannan Wu et al. "Alginate-aker injectable composite hydrogels promoted irregular bone regeneration through stem cell recruitment and osteogenic differentiation". Journal of Materials Chemistry B 6, n.º 13 (2018): 1951–64. http://dx.doi.org/10.1039/c7tb03315j.
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Siddiqi, Ayesha, Marie P. Parsons, John L. Lewis, John P. Monson, Graham R. Williams y Jacky M. Burrin. "TR Expression and Function in Human Bone Marrow Stromal and Osteoblast-Like Cells". Journal of Clinical Endocrinology & Metabolism 87, n.º 2 (1 de febrero de 2002): 906–14. http://dx.doi.org/10.1210/jcem.87.2.8226.
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Thyroid hormones influence both bone formation and bone resorption. In vitro studies demonstrate direct effects of thyroid hormones on cells of the osteoblast lineage. Transcriptional regulation by thyroid hormones is mediated by ligand-dependent transcription factors called TRs. The three main T3-binding TR isoforms are TRα1, TRβ1, and TRβ2. TRs have been identified in cells of the osteoblast lineage, but it is still not known whether TR isoform expression differs in primary cultures of human osteoblasts. We used immunocytochemistry, Western blotting, nuclear binding assays, and transient transfection studies to examine the expression of functional TR isoforms in primary cultures of osteoblasts (hOb) derived from explants of trabecular bone, in human bone marrow stromal cells (hBMS), which are believed to be the source of osteoblast progenitor cells, and for comparison in the transformed human osteosarcoma cell lines MG63 and SaOs-2. TRα1, TRβ1, and TRβ2 proteins were expressed in all cells, although expression was greatest in MG63 > hBMS > SaOs-2 > hOb. Differences between isoforms were also apparent, with TRα1> TRβ1 > TRβ2 in all cell types. Incubation with [125I]T3 confirmed reversible T3 binding to cell nuclei. Specific binding was greatest in MG63 > hBMS > SaOs-2 > hOb. Finally, endogenous TR activity was determined in transfections using a thyroid hormone response element derived from the rat GH gene linked to the luciferase reporter gene. In MG63 and hBMS cells T3 treatment increased luciferase activity 5.5 ± 0.7-fold (P < 0.05), confirming the presence of endogenous receptors. In SaOs-2 and hOb cells, T3 treatment had no effect on thyroid hormone response element-thymidine kinase-luciferase expression, suggesting that in these cells TR expression was too low to be detected. These results indicate that three main TR isoforms are expressed in cells of the human osteoblast lineage, but that expression and endogenous TR activity are predominantly present in hBMS cells. Whether there are distinct mechanisms of thyroid hormone action mediated by TRα1, TRβ1, and TRβ2 in hOb and hBMS cells remains to be shown.
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Siddiqi, A., JM Burrin, DF Wood y JP Monson. "Tri-iodothyronine regulates the production of interleukin-6 and interleukin-8 in human bone marrow stromal and osteoblast-like cells". Journal of Endocrinology 157, n.º 3 (1 de junio de 1998): 453–61. http://dx.doi.org/10.1677/joe.0.1570453.
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Hyperthyroidism is associated with increased bone resorption but the mechanisms by which thyroid hormone (T3) affects bone cell metabolism remain unclear. Recently it has been suggested that T3 stimulates osteoclastic resorption indirectly through the release of soluble mediators from osteoblasts. The aim of the present study was to investigate whether the T3-induced increase in bone resorption could be due to the regulation of cytokine production by human osteoblasts (hOb). The effects of T3 (1, 10, 100 nM) and IL-1 beta (100 U/ml) as the positive control were examined on cytokine protein release and mRNA levels in cultured hOb cell lines (MG63, SaOs-2), primary hOb and human bone marrow stromal (hBMS) cells. T3 increased IL-6 and IL-8 mRNA levels as well as IL-6 and IL-8 protein release into the culture media from MG63 and hBMS cells in a time- and dose-dependent manner. The maximal effect on protein release in hBMS cells occurred at 24 h with a dose of T3 10 nM (IL-6 5.5 +/- 1.1-fold above controls; IL-8 3.7 +/- 0.5-fold above controls, P < 0.05). At the same time, mRNA levels in hBMS cells were increased 6.2 +/- 0.8-fold for IL-6 (P < 0.05) and 5.7 +/- 0.8-fold for IL-8 (P < 0.05). Similar results were obtained in MG63 cells but no response was seen in SaOs-2 or hOb cells despite measurable basal production. Nor was there detectable regulation of IL-1 beta, IL-3, IL-11, IL-4 or granulocyte macrophage-colony stimulating factor by T3 in any cell type. In conclusion, T3 increases IL-6 and IL-8 production by MG63 and hBMS cells, suggesting that IL-6 and IL-8 may be T3-regulated genes in osteoblasts.
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Szepes, Mónika, Zsolt Benkő, Attila Cselenyák, Kai Michael Kompisch, Udo Schumacher, Zsombor Lacza y Levente Kiss. "Comparison of the Direct Effects of Human Adipose- and Bone-Marrow-Derived Stem Cells on Postischemic Cardiomyoblasts in anIn VitroSimulated Ischemia-Reperfusion Model". Stem Cells International 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/178346.
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Regenerative therapies hold a promising and exciting future for the cure of yet untreatable diseases, and mesenchymal stem cells are in the forefront of this approach. However, the relative efficacy and the mechanism of action of different types of mesenchymal stem cells are still incompletely understood. We aimed to evaluate the effects of human adipose- (hASC) and bone-marrow-derived stem cells (hBMSCs) and adipose-derived stem cell conditioned media (ACM) on the viability of cardiomyoblasts in anin vitroischemia-reperfusion (I-R) model. Flow cytometric viability analysis revealed that both cell treatments led to similarly increased percentages of living cells, while treatment with ACM did not (I-R model:12.13±0.75%; hASC:24.66±2.49%; hBMSC:25.41±1.99%; ACM:13.94±1.44%). Metabolic activity measurement (I-R model:0.065±0.033; hASC:0.652±0.089; hBMSC:0.607±0.059; ACM:0.225±0.013; arbitrary units) and lactate dehydrogenase assay (I-R model:0.225±0.006; hASC:0.148±0.005; hBMSC:0.146±0.004; ACM:0.208±0.009; arbitrary units) confirmed the flow cytometric results while also indicated a slight beneficial effect of ACM. Our results highlight that mesenchymal stem cells have the same efficacy when used directly on postischemic cells, and differences found between them in preclinical and clinical investigations are rather related to other possible causes such as their immunomodulatory or angiogenic properties.
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Pan, Xi, Zibo Zuo, Longlong Zhang y Tingsheng Zhao. "Research on Dynamic Monitoring and Early Warning of the High-Rise Building Machine during the Climbing Stage". Advances in Civil Engineering 2023 (31 de octubre de 2023): 1–12. http://dx.doi.org/10.1155/2023/9326791.
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High-rise building machines (HBMs) are commonly used for the construction of super-high skyscrapers. Monitoring and early warning are critical to ensure the safety of giant HBMs during dynamic climbing. However, no indicators or control methods directly reflect the dynamic safety state of such large structures. In this study, the key factors influencing the climbing altitude were systematically analyzed and a method for the dynamic monitoring and early warning of HBMs during climbing was proposed. This approach is innovative for monitoring 3D attitude in real time using a string of fiber grating-level sensors mounted on the main bearing surface of the HBM. Three-level early warning indicators and control methods that reflect the safety status of the HBM during dynamic climbing have been established. The method was successfully applied to a 356-m tall high-rise project, the Shenzhen Xinghe Yabao Building. Results demonstrate that the proposed method can monitor and control the safety state of the HBM climbing process more accurately than current methods in real time. In addition, it significantly reduces the impact of factors such as preclimbing differential deformation and climbing attitude recognition on the accuracy of the HBM climbing control. This guarantees the safe management of HBMs and the efficient construction of super high-rise buildings. The results of this study can also be widely applied to safety monitoring of the dynamic operation of giant construction machinery.
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Yuan, Lunzhi, Jing Jiang, Xuan Liu, Yali Zhang, Liang Zhang, Jiaojiao Xin, Kun Wu et al. "HBV infection-induced liver cirrhosis development in dual-humanised mice with human bone mesenchymal stem cell transplantation". Gut 68, n.º 11 (30 de enero de 2019): 2044–56. http://dx.doi.org/10.1136/gutjnl-2018-316091.
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ObjectiveDeveloping a small animal model that accurately delineates the natural history of hepatitis B virus (HBV) infection and immunopathophysiology is necessary to clarify the mechanisms of host-virus interactions and to identify intervention strategies for HBV-related liver diseases. This study aimed to develop an HBV-induced chronic hepatitis and cirrhosis mouse model through transplantation of human bone marrow mesenchymal stem cells (hBMSCs).DesignTransplantation of hBMSCs into Fah-/-Rag2-/-IL-2Rγc-/- SCID (FRGS) mice with fulminant hepatic failure (FHF) induced by hamster-anti-mouse CD95 antibody JO2 generated a liver and immune cell dual-humanised (hBMSC-FRGS) mouse. The generated hBMSC-FRGS mice were subjected to assessments of sustained viremia, specific immune and inflammatory responses and liver pathophysiological injury to characterise the progression of chronic hepatitis and cirrhosis after HBV infection.ResultsThe implantation of hBMSCs rescued FHF mice, as demonstrated by robust proliferation and transdifferentiation of functional human hepatocytes and multiple immune cell lineages, including B cells, T cells, natural killer cells, dendritic cells and macrophages. After HBV infection, the hBMSC-FRGS mice developed sustained viremia and specific immune and inflammatory responses and showed progression to chronic hepatitis and liver cirrhosis at a frequency of 55% after 54 weeks.ConclusionThis new humanised mouse model recapitulates the liver cirrhosis induced by human HBV infection, thus providing research opportunities for understanding viral immune pathophysiology and testing antiviral therapies in vivo.
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Sun, Suwan, Lunzhi Yuan, Zhanglu An, Dongyan Shi, Jiaojiao Xin, Jing Jiang, Keke Ren et al. "DLL4 restores damaged liver by enhancing hBMSC differentiation into cholangiocytes". Stem Cell Research 47 (agosto de 2020): 101900. http://dx.doi.org/10.1016/j.scr.2020.101900.
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LEAL, ÂNGELA M. "hBMSC-TERT in Cutaneous Diabetic Wound—Histological Analysis and Metalloproteinases". Diabetes 67, Supplement 1 (mayo de 2018): 588—P. http://dx.doi.org/10.2337/db18-588-p.
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Qi, Jin, Yali Zhou, Zuoyi Jiao, Xu Wang, Yang Zhao, Yangbin Li, Huijuan Chen, Luxi Yang, Hongwen Zhu y Yumin Li. "Exosomes Derived from Human Bone Marrow Mesenchymal Stem Cells Promote Tumor Growth Through Hedgehog Signaling Pathway". Cellular Physiology and Biochemistry 42, n.º 6 (2017): 2242–54. http://dx.doi.org/10.1159/000479998.
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Background/Aims: Mesenchymal stem/stromal cells (MSCs) are known to home to sites of tumor microenvironments where they participate in the formation of the tumor microenvironment and to interplay with tumor cells. However, the potential functional effects of MSCs on tumor cell growth are controversial. Here, we, from the view of bone marrow MSC-derived exosomes, study the molecular mechanism of MSCs on the growth of human osteosarcoma and human gastric cancer cells. Methods: MSCs derived from human bone marrow (hBMSCs) were isolated and cultured in complete DMEM/F12 supplemented with 10% exosome-depleted fetal bovine serum and 1% penicillin-streptomycin, cell culture supernatants containing exosomes were harvested and exosome purification was performed by ultracentrifugation. Osteosarcoma (MG63) and gastric cancer (SGC7901) cells, respectively, were treated with hBMSC-derived exosomes in the presence or absence of a small molecule inhibitor of Hedgehog pathway. Cell viability was measured by transwell invasion assay, scratch migration assay and CCK-8 test. The expression of the signaling molecules Smoothened, Patched-1, Gli1 and the ligand Shh were tested by western blot and RT-PCR. Results: In this study, we found that hBMSC-derived exosomes promoted MG63 and SGC7901 cell growth through the activation of Hedgehog signaling pathway. Inhibition of Hedgehog signaling pathway significantly suppressed the process of hBMSC-derived exosomes on tumor growth. Conclusion: Our findings demonstrated the new roles of hedgehog signaling pathway in the hBMSCs-derived exosomes induced tumor progression.
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Parratt, Kirsten, Madeline Smerchansky, Qwantayvious Stiggers y Krishnendu Roy. "Correction: Effect of hydrogel material composition on hBMSC differentiation into zone-specific neo-cartilage: engineering human articular cartilage-like tissue with spatially varying properties". Journal of Materials Chemistry B 6, n.º 14 (2018): 2159. http://dx.doi.org/10.1039/c8tb90034e.
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Correction for ‘Effect of hydrogel material composition on hBMSC differentiation into zone-specific neo-cartilage: engineering human articular cartilage-like tissue with spatially varying properties’ by Kirsten Parratt et al., J. Mater. Chem. B, 2017, 5, 6237–6248.
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Germanetti, Filippo, Dario Fiumarella, Giovanni Belingardi y Alessandro Scattina. "Injury Criteria for Vehicle Safety Assessment: A Review with a Focus Using Human Body Models". Vehicles 4, n.º 4 (7 de octubre de 2022): 1080–95. http://dx.doi.org/10.3390/vehicles4040057.
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This paper aims at providing an overview of the most used injury criteria (IC) and injury metrics for the study of the passive safety of vehicles. In particular, the work is focused on the injury criteria that can be adopted when finite element simulations and Human Body Models (HBMs) are used. The HBMs will result in a fundamental instrument for studying the occupant’s safety in Autonomous Vehicles (AVs) since they allow the analysis of a larger variety of configurations compared to the limitations related to the traditional experimental dummies. In this work, the most relevant IC are reported and classified based on the body segments. In particular, the head, the torso, the spine, the internal organs, and the lower limbs are here considered. The applicability of the injury metrics to the analyses carried out with the HBMs is also discussed. The paper offers a global overview of the injury assessment useful to choose the injury criteria for the study of vehicle passive safety. To this aim, tables of the presented criteria are also reported to provide the available metrics for the considered body damage.
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Eccles, Jacquelynne S. y Ming-Te Wang. "What motivates females and males to pursue careers in mathematics and science?" International Journal of Behavioral Development 40, n.º 2 (22 de noviembre de 2015): 100–106. http://dx.doi.org/10.1177/0165025415616201.
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Drawing on Eccles’ expectancy-value model of achievement-related choices, we examined the personal aptitudes and motivational beliefs at 12th grade that move individuals toward or away from science, technology, engineering, and mathematics (STEM) occupations at age 29. In the first set of analyses, occupational and lifestyle values, math ability self-concepts, family demographics, and high school course-taking more strongly predicted both individual and gender differences in the likelihood of entering STEM careers than math scores on the Differential Aptitude Test. In the second set of analyses, individual and gender differences in career decisions within STEM disciplines (health, biological, and medical sciences (HBMS) versus mathematics, physical, engineering, and computer sciences (MPECS)) were best predicted by occupational values (i.e. preferences for work that were people oriented and altruistic predicted entrance into HBMS instead of MPECS careers). Females were less likely to hold the beliefs that predicted selection of STEM in general, but those who did choose STEM were more likely to select HBMS than MPECS. One Sentence Summary: Gender differences in selecting STEM related and health, biological, and medical occupations result primarily from gender differences in occupational and lifestyle values.
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Chung, Tze-Wen, Weng-Pin Chen, Pei-Wen Tai, Hsin-Yu Lo y Ting-Ya Wu. "Roles of Silk Fibroin on Characteristics of Hyaluronic Acid/Silk Fibroin Hydrogels for Tissue Engineering of Nucleus Pulposus". Materials 13, n.º 12 (17 de junio de 2020): 2750. http://dx.doi.org/10.3390/ma13122750.
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Silk fibroin (SF) and hyaluronic acid (HA) were crosslinked by horseradish peroxidase (HRP)/H2O2, and 1,4-Butanediol di-glycidyl ether (BDDE), respectively, to produce HA/SF-IPN (interpenetration network) (HS-IPN) hydrogels. HS-IPN hydrogels consisted of a SF strain with a high content of tyrosine (e.g., strain A) increased viscoelastic modules compared with those with low contents (e.g., strain B and C). Increasing the quantities of SF in HS-IPN hydrogels (e.g., HS7-IPN hydrogels with weight ratio of HA/SF, 5:7) increased viscoelastic modules of the hydrogels. In addition, the mean pores size of scaffolds of the model hydrogels were around 38.96 ± 5.05 μm which was between those of scaffolds H and S hydrogels. Since the viscoelastic modulus of the HS7-IPN hydrogel were similar to those of human nucleus pulposus (NP), it was chosen as the model hydrogel for examining the differentiation of human bone marrow-derived mesenchymal stem cell (hBMSC) to NP. The differentiation of hBMSC induced by transforming growth factor β3 (TGF-β3) in the model hydrogels to NP cells for 7 d significantly enhanced the expressions of glycosaminoglycan (GAG) and collagen type II, and gene expressions of aggrecan and collagen type II while decreased collagen type I compared with those in cultural wells. In summary, the model hydrogels consisted of SF of strain A, and high concentrations of SF showed the highest viscoelastic modulus than those of others produced in this study, and the model hydrogels promoted the differentiation of hBMSC to NP cells.
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Hamidi, Samer. "Integrating E-Learning into Health Care Education : The Case of HBMSU". International Journal of Excellence in Education 6, n.º 1 (2014): 1–12. http://dx.doi.org/10.12816/0010842.
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Chung, Tze-Wen, Hsin-Yu Lo, Tzung-Han Chou, Jan-Hou Chen y Shoei-Shen Wang. "Promoting Cardiomyogenesis of hBMSC with a Forming Self-Assembly hBMSC Microtissues/HA-GRGD/SF-PCL Cardiac Patch Is Mediated by the Synergistic Functions of HA-GRGD". Macromolecular Bioscience 17, n.º 3 (28 de septiembre de 2016): 1600173. http://dx.doi.org/10.1002/mabi.201600173.
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Long, Yazhou, Katrin Bundkirchen, Pascal Gräff, Christian Krettek, Sandra Noack y Claudia Neunaber. "Cytological Effects of Serum Isolated from Polytraumatized Patients on Human Bone Marrow-Derived Mesenchymal Stem Cells". Stem Cells International 2021 (28 de noviembre de 2021): 1–18. http://dx.doi.org/10.1155/2021/2612480.
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Due to their immunomodulatory and regenerative capacity, human bone marrow-derived mesenchymal stem cells (hBMSCs) are promising in the treatment of patients suffering from polytrauma. However, few studies look at the effects of sera from polytraumatized patients on hBMSCs. The aim of this study was to explore changes in hBMSC properties in response to serum from polytrauma patients taken at different time points after the trauma incident. For this, sera from 84 patients with polytrauma (collected between 2010 and 2020 in our department) were used. In order to test the differential influence on hBMSC, sera from the 1st (D1), 5th (D5), and 10th day (D10) after polytrauma were pooled, respectively. As a control, sera from three healthy donors (HS), matched with respect to age and gender to the polytrauma group, were collected. Furthermore, hBMSCs from four healthy donors were used in the experiments. The pooled sera of HS, D1, D5, and D10 were analyzed by multicytokine array for pro-/anti-inflammatory cytokines. Furthermore, the influence of the different sera on hBMSCs with respect to cell proliferation, colony forming unit-fibroblast (CFU-F) assay, cell viability, cytotoxicity, cell migration, and osteogenic and chondrogenic differentiation was analyzed. The results showed that D5 serum significantly reduced hBMSC cell proliferation capacity compared with HS and increased the proportion of dead cells compared with D1. However, the frequency of CFU-F was not reduced in polytrauma groups compared with HS, as well as the other parameters. The serological effect of polytrauma on hBMSCs was related to the time after trauma. It is disadvantageous to use BMSCs in polytraumatized patients at least until the fifth day after polytrauma as obvious cytological changes could be found at that time point. However, it is promising to use hBMSCs to treat polytrauma after five days, combined with the concept of “Damage Control Orthopedics” (DCO).
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Sun, Hao, Zhiyu Huang, Peihui Wu, Zongkun Chang, Weiming Liao y Zhiqi Zhang. "CDK6 and miR-320c Co-Regulate Chondrocyte Catabolism Through NF-κB Signaling Pathways". Cellular Physiology and Biochemistry 51, n.º 2 (2018): 909–23. http://dx.doi.org/10.1159/000495392.
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Background/Aims: Cyclin-dependent kinase 6 (CDK6) regulates inflammatory response and cell differentiation. This study sought to determine whether CDK6 and miR-320c co-regulate chondrogenesis and inflammation. Methods: Utilizing quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC), CDK6 and miR-320c expression were assessed in a micromass culture of human bone mesenchymal stem cells that underwent chondrogenesis in vitro as well as in chondrocytes from E16.5 mouse forelimbs. Normal chondrocytes were transfected with miR-320c mimic, miR-320c inhibitor, or CDK6-siRNA. Luciferase reporter assay results confirmed that miR-320c directly targets CDK6 by interacting with the 3′-untranslated region (3′-UTR) of its mRNA. qRT-PCR, Western blotting, and Cell Counting Kit-8 were subsequently used to evaluate the effects of miR-320c overexpression and CDK6 inhibition on inflammatory factor expression, as well as to investigate the effects of NF-kB and MAPK signaling pathway activation on IL-1β-induced chondrocyte inflammation. Results: Our results show that miR-320c expression increased during the middle stage and decreased during the late stage of hBMSC chondrogenic differentiation. In contrast, CDK6 expression decreased during the middle stage and increased during the late stage of hBMSC chondrogenic differentiation. Moreover, CDK6 expression increased in severe OA cartilage and in hypertrophic chondrocytes of mouse forelimbs at E16.5. Results of the luciferase reporter assay showed that miR-320c modulated CDK6 expression by binding to the 3′-UTR of its mRNA. miR-320c overexpression and CDK6 inhibition repressed IL-1β-induced expression of inflammatory factors and regulated the NF-kB signaling pathway. Conclusion: CDK6 and miR-320c co-regulate hBMSC chondrogenesis and IL-1β-induced chondrocyte inflammation through the NF-kB signaling pathway, suggesting that miR-320c and CDK6 inhibitors can be used to repress catabolism in human chondrocytes.
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Heo, S. J., S. E. Kim, Yong Taek Hyun, D. H. Kim, Hyang Mi Lee, Yeong Maw Hwang, S. A. Park y Jung Woog Shin. "In Vitro Evaluation of Poly ε-Caprolactone/Hydroxyapatite Composite as Scaffolds for Bone Tissue Engineering with Human Bone Marrow Stromal Cells". Key Engineering Materials 342-343 (julio de 2007): 369–72. http://dx.doi.org/10.4028/www.scientific.net/kem.342-343.369.
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This study evaluated the potential of the PCL (poly -caprolactone)/HA(Hydroxyapatite) composite materials as a scaffold for bone regeneration. For this, we fabricated scaffolds utilizing salt leaching method. The PCL/HA composite scaffolds were prepared with various HA contents (20wt%, 40wt%, 60 wt %). To ensure the potential for the scaffolds, porosity tests were conducted along with SEM observations. The porosity decreased with the increase of the contents of HA particles. The porosity of the composite with the highest contents of HA was still adoptable (~85%). In addition, the PCL/HA composite scaffolds were evaluated for their ability of osteogenic differentiation with human bone marrow stromal cell (hBMSC) in vitro. Alkaline phosphatase (ALP) activity, markers for osteoblastic differentiation, and total protein contents were evaluated in hBMSCs following 14 days of cultivation. The addition of HA particles enhanced proliferation of hBMSC during the test. Also, the differentiation ability of the cells was increased as HA particles were added. In this study, we concluded that PCL/HA composite scaffolds has great potential as a scaffold for bone tissue engineering.
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Sturm, Lisa, Bettina Schwemberger, Ursula Menzel, Sonja Häckel, Christoph E. Albers, Christian Plank, Jaap Rip et al. "In Vitro Evaluation of a Nanoparticle-Based mRNA Delivery System for Cells in the Joint". Biomedicines 9, n.º 7 (8 de julio de 2021): 794. http://dx.doi.org/10.3390/biomedicines9070794.
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Biodegradable and bioresponsive polymer-based nanoparticles (NPs) can be used for oligonucleotide delivery, making them a promising candidate for mRNA-based therapeutics. In this study, we evaluated and optimized the efficiency of a cationic, hyperbranched poly(amidoamine)s-based nanoparticle system to deliver tdTomato mRNA to primary human bone marrow stromal cells (hBMSC), human synovial derived stem cells (hSDSC), bovine chondrocytes (bCH), and rat tendon derived stem/progenitor cells (rTDSPC). Transfection efficiencies varied among the cell types tested (bCH 28.4% ± 22.87, rTDSPC 18.13% ± 12.07, hBMSC 18.23% ± 14.80, hSDSC 26.63% ± 8.81) and while an increase of NPs with a constant amount of mRNA generally improved the transfection efficiency, an increase of the mRNA loading ratio (2:50, 4:50, or 6:50 w/w mRNA:NPs) had no impact. However, metabolic activity of bCHs and rTDSPCs was significantly reduced when using higher amounts of NPs, indicating a dose-dependent cytotoxic response. Finally, we demonstrate the feasibility of transfecting extracellular matrix-rich 3D cell culture constructs using the nanoparticle system, making it a promising transfection strategy for musculoskeletal tissues that exhibit a complex, dense extracellular matrix.
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Sakuragi, Makoto, Takashi Kitajima, Teruyuki Nagamune y Yoshihiro Ito. "Recombinant hBMP4 incorporated with non-canonical amino acid for binding to hydroxyapatite". Biotechnology Letters 33, n.º 9 (5 de mayo de 2011): 1885–90. http://dx.doi.org/10.1007/s10529-011-0637-1.
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Cordonnier, Thomas, Alain Langonné, Pierre Corre, Audrey Renaud, Luc Sensebé, Philippe Rosset, Pierre Layrolle y Jérôme Sohier. "Osteoblastic differentiation and potent osteogenicity of three-dimensional hBMSC-BCP particle constructs". Journal of Tissue Engineering and Regenerative Medicine 8, n.º 5 (11 de junio de 2012): 364–76. http://dx.doi.org/10.1002/term.1529.
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Schäck, Luisa Marilena, Manuela Buettner, Alexander Wirth, Claudia Neunaber, Christian Krettek, Andrea Hoffmann y Sandra Noack. "Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype". Stem Cells International 2016 (2016): 1–13. http://dx.doi.org/10.1155/2016/1319578.
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Human bone marrow-derived stromal cells (hBMSCs) derived from the adult organism hold great promise for diverse settings in regenerative medicine. Therefore a more complete understanding of hBMSC biology to fully exploit the cells’ potential for clinical settings is important. The protein CD24 has been reported to be involved in a diverse range of processes such as cancer, adaptive immunity, inflammation, and autoimmune diseases in other cell types. Its expression in hBMSCs, which has not yet been analyzed, may add an important aspect in the understanding of hBMSC biology. The present study therefore analyzes the expression, regulation, and functional implication of the surface protein CD24 in hBMSCs. Methods used are stimulation studies with TGF beta as well as shRNA-mediated knockdown and overexpression of CD24 followed by microarray, immunocytochemistry, and flow cytometric analyses. To our knowledge, we demonstrate for the first time that the expression of CD24 is an inherent property of hBMSCs. Importantly, the data links the upregulation of CD24 to the adoption of a myofibroblast-like gene expression pattern in hBMSCs. We demonstrate that CD24 is an important modulator in transforming growth factor beta 3 (TGFβ3) signaling with a reciprocal regulatory relationship between these two proteins.
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Heinemann, Christiane, Frauke Buchner, Poh Soo Lee, Anne Bernhardt, Benjamin Kruppke, Hans-Peter Wiesmann y Vera Hintze. "Effects of Gamma Irradiation and Supercritical Carbon Dioxide Sterilization on Methacrylated Gelatin/Hyaluronan Hydrogels". Journal of Functional Biomaterials 14, n.º 6 (8 de junio de 2023): 317. http://dx.doi.org/10.3390/jfb14060317.
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Biopolymer hydrogels have become an important group of biomaterials in experimental and clinical use. However, unlike metallic or mineral materials, they are quite sensitive to sterilization. The aim of this study was to compare the effects of gamma irradiation and supercritical carbon dioxide (scCO2) treatment on the physicochemical properties of different hyaluronan (HA)- and/or gelatin (GEL)-based hydrogels and the cellular response of human bone marrow-derived mesenchymal stem cells (hBMSC). Hydrogels were photo-polymerized from methacrylated HA, methacrylated GEL, or a mixture of GEL/HA. The composition and sterilization methods altered the dissolution behavior of the biopolymeric hydrogels. There were no significant differences in methacrylated GEL release but increased methacrylated HA degradation of gamma-irradiated samples. Pore size/form remained unchanged, while gamma irradiation decreased the elastic modulus from about 29 kPa to 19 kPa compared to aseptic samples. HBMSC proliferated and increased alkaline phosphatase activity (ALP) particularly in aseptic and gamma-irradiated methacrylated GEL/HA hydrogels alike, while scCO2 treatment had a negative effect on both proliferation and osteogenic differentiation. Thus, gamma-irradiated methacrylated GEL/HA hydrogels are a promising base for multi-component bone substitute materials.
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Şermet Kaya, Şenay y Yeter Kitiş. "Elderly diabetes patients’ health beliefs about care and treatment for diabetes". Journal of Human Sciences 15, n.º 1 (25 de enero de 2018): 51. http://dx.doi.org/10.14687/jhs.v15i1.4903.
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Purpose: This descriptive study aimed to assess elderly diabetes patients’ health beliefs about care and treatment for diabetes.Methods: The universe of the study consists of 1176 diabetic patients aged 65 years and over who are registered to eight family health centers affiliated to Mezitli district of Mersin province. In the sample, it was planned to reach the elderly between 165-330. As a result, 280 elders were reached. After obtaining the necessary permissions from the related institutions, data were collected with Descriptive Characteristics Form and HBMS for Diabetes Patients in 2012 and analyzed with nonparametric tests.Results: Of 280 patients, 55.7% were male and 60% were aged 65-69. The median value for HBMS showed that the patients had a negative health belief. The patients with higher education levels and those receiving information about diabetes had higher median of values for both the scale and its subscales, those checking their blood glucose had high median of values for the scale and the subscale perceived benefits and barriers, those complying with nutrition therapy had higher median of values for perceived barriers and recommended health behaviours, those having regular check-ups had higher median of values for perceived barriers and those doing exercise regularly had higher median of values for perceived benefits (p<0.05).Conclusion: Elderly diabetes patients should be offered education about self management and HBMS for Diabetes Patients should be used to determine educational needs and to evaluate effectiveness of education offered to help diabetes patients to develop positive health beliefs.
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Tsiftsoglou, Stefanos A. "Heme Interactions as Regulators of the Alternative Pathway Complement Responses and Implications for Heme-Associated Pathologies". Current Issues in Molecular Biology 45, n.º 6 (16 de junio de 2023): 5198–214. http://dx.doi.org/10.3390/cimb45060330.
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Heme (Fe2+-protoporphyrin IX) is a pigment of life, and as a prosthetic group in several hemoproteins, it contributes to diverse critical cellular processes. While its intracellular levels are tightly regulated by networks of heme-binding proteins (HeBPs), labile heme can be hazardous through oxidative processes. In blood plasma, heme is scavenged by hemopexin (HPX), albumin and several other proteins, while it also interacts directly with complement components C1q, C3 and factor I. These direct interactions block the classical pathway (CP) and distort the alternative pathway (AP). Errors or flaws in heme metabolism, causing uncontrolled intracellular oxidative stress, can lead to several severe hematological disorders. Direct interactions of extracellular heme with alternative pathway complement components (APCCs) may be implicated molecularly in diverse conditions at sites of abnormal cell damage and vascular injury. In such disorders, a deregulated AP could be associated with the heme-mediated disruption of the physiological heparan sulphate–CFH coat of stressed cells and the induction of local hemostatic responses. Within this conceptual frame, a computational evaluation of HBMs (heme-binding motifs) aimed to determine how heme interacts with APCCs and whether these interactions are affected by genetic variation within putative HBMs. Combined computational analysis and database mining identified putative HBMs in all of the 16 APCCs examined, with 10 exhibiting disease-associated genetic (SNPs) and/or epigenetic variation (PTMs). Overall, this article indicates that among the pleiotropic roles of heme reviewed, the interactions of heme with APCCs could induce differential AP-mediated hemostasis-driven pathologies in certain individuals.