Tesis sobre el tema "Genetically modified foods – Fiction"
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Hall, Clare R. "Genetically modified food and crops : perceptions of risks". Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4719.
Texto completoScholderer, Joachim. "Consumer attitudes towards genetically modified foods in Europe : structure and changeability". Phd thesis, Universität Potsdam, 2004. http://opus.kobv.de/ubp/volltexte/2006/624/.
Texto completoBased on classical approaches in attitude research and modern theories of social cognition, a general model of the structure, function and dynamics of whole systems of attitudes is developed. The predictions of the model are empirically tested based on an attitude survey (N = 2000) and two attitude change experiments (N = 1400 and N = 750). All three studies were conducted in parallel in four EU member states. The results show that consumer attitudes towards genetically modified foods are embedded into a structured system of general socio-political attitudes. The system operates as a schema through which consumers form global evaluations of the technology. Specific risk and benefit judgments are mere epiphenomena of this process. Risk-benefit trade-offs, as often presupposed in the literature, do not appear to enter the process. The attitudes have a value-expressive function; their purpose is not just a temporary reduction of complexity. These properties render the system utterly resistant to communicative interventions. At the same time, it exerts stong anchoring effects on the processing of new information. Communication of benefit arguments can trigger boomerang effects and backfire on the credibility of the communicator when the arguments contrast with preexisting attitudes held by the consumer. Only direct sensory experience with high-quality products can partially bypass the system and lead to the formation of alternative attitude structures. Therefore, the recommended market introduction strategy for genetically modified foods is the simultaneous and coordinated launch of many high-quality products. Point of sale promotions should be the central instrument. Information campaigns, on the other hand, are not likely to have an effect on the product and technology acceptance of European consumers.
Gentechnisch veränderte Lebensmittel haben sich im Lauf der letzten zwei Jahrzehnte zu einem Symbol verbraucherpolitischer Auseinandersetzungen in Europa entwickelt. Mit der Aufhebung des Quasi-Moratoriums im Mai 2004 ist der Weg zum Markt zwar prinzipiell freigeschaltet, Strategien der Produkteinführung fehlen jedoch. Ziel der Arbeit ist, potenzielle Hindernisse im Bereich der Verbraucherakzeptanz auszuloten und Wege aufzuzeigen, wie Vorurteile gegenüber diesen Produkten abgebaut werden könnten. Nach einem historischen Abriss der Gentechnikdebatte in Europa wird zunächst die existierende Literatur zur Verbraucherakzeptanz diskutiert. Obwohl die bisherige Forschung in ihren Ergebnissen konvergiert, sind grundsätzlichere Fragen weitgehend ungelöst geblieben.
Auf der Grundlage klassischer Ansätze der Einstellungsforschung und moderner Theorien sozialer Kognition wird daher ein allgemeines Modell der Struktur, Funktion und Dynamik ganzer Einstellungssysteme entworfen. Die Vorhersagen des Modells werden anhand einer Einstellungsbefragung (N = 2000) und zweier Einstellungsänderungsexperimente (N = 1400 und N = 750) empirisch überprüft. Alle drei Studien wurden parallel in vier EU-Mitgliedsstaaten durchgeführt. Die Ergebnisse zeigen, dass Verbrauchereinstellungen zu gentechnisch veränderten Lebensmitteln in ein strukturiertes System allgemeiner soziopolitischer Einstellungen eingebettet sind. Das System fungiert als Schema, anhand dessen eine globale Bewertung der Gentechnik vorgenommen wird. Spezifische Nutzen- und Risikourteile sind lediglich Epiphänomene dieses Prozesses. Nutzen-Risiko-Abwägungen, wie sie in der Literatur oft unterstellt werden, finden dabei offenbar nicht statt. Die Einstellungen haben eine wertexpressive Funktion, dienen also nicht nur der vordergründigen Reduktion von Komplexität. Diese Eigenschaften machen das System ausgesprochen resistent gegenüber Versuchen der kommunikativen Beeinflussung. Gleichzeitig übt es eine starke Ankerwirkung auf die Verarbeitung neuer Informationen aus. Kommunikation von Nutzenargumenten zu gentechnisch veränderten Lebensmitteln kann zu Bumerangeffekten auf die Glaubwürdigkeit eines Kommunikators führen, wenn die Argumente den Voreinstellungen der Verbraucher widersprechen. Lediglich direkte sensorische Erfahrung mit qualitativ hochwertigen Produkten kann zu einer teilweisen Entkoppelung des Systems und damit zum Aufbau alternativer Einstellungsstrukturen führen. Als optimale Markteinführungsstrategie für gentechnisch veränderte Lebensmittel wird daher die gleichzeitige und koordinierte Einführung vieler hochwertiger Einzelprodukte empfohlen. Zentrales Instrument sollten Aktionen am Point of Sale sein. Vorbereitende Informationskampagnen werden dagegen voraussichtlich keine Wirkungen auf die Produkt- and Technikakzeptanz europäischer Verbraucher haben.
Plunkett, Marni. "Are genetically modified foods good?, the welfare implications of agricultural biotechnology". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape3/PQDD_0015/MQ49580.pdf.
Texto completoAtkinson, Emily. "Habermas, biopower, and the regulation of genetically modified crops and foods". Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/45129.
Texto completoMoser, Celeste Laurana. "Public opinion and public engagement with genetically modified foods : a qualitative study". PDXScholar, 2010. https://pdxscholar.library.pdx.edu/open_access_etds/4279.
Texto completoGao, Rong y 高溶. "A systematic review of the use of genetically modified food in China". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193785.
Texto completopublished_or_final_version
Public Health
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Master of Public Health
Cuellar, Jose. "Mexican consumer preferences for biotechnology and retail food outlets". Online access for everyone, 2004. http://www.dissertations.wsu.edu/Thesis/Fall2004/J%5FCuellar%5F121704.pdf.
Texto completoKaneko, Naoya. "Three essays on economic valuation of consumer preferences on genetically modified foods". Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1127328876.
Texto completoTitle from first page of PDF file. Document formatted into pages; contains xiv, 168 p.; also includes graphics (some col.). Includes bibliographical references (p. 161-168). Available online via OhioLINK's ETD Center
Magnusson, Maria. "Consumer Perception of Organic and Genetically Modified Foods : Health and Environmental Considerations". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis :, 2004. http://publications.uu.se/theses/abstract.xsql?dbid=4218.
Texto completoCostello, Alexandra Jane. "The effect of GM label variation on perceptions of genetically modified foods". Thesis, University of Plymouth, 2007. http://hdl.handle.net/10026.1/1983.
Texto completoKhromov, I. "Genetic modified food". Thesis, Сумський державний університет, 2013. http://essuir.sumdu.edu.ua/handle/123456789/33677.
Texto completoHu, Rongrong 1970. "Labeling of genetically modified organisms and the producer's negative labeling decision under a voluntary labeling regime". Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=112638.
Texto completoShirai, Tomoko. "The WTO and the mandatory labeling of generically modified foods /". Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81235.
Texto completoAydin, Gamze. "Detection Of Genetically Modified Maize Via Polymerase Chain Reaction". Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12605495/index.pdf.
Texto completoJohnson, Adam Michael. "Pricing Genetically Modified Output Traits and Effects on Competing Technologies". Thesis, North Dakota State University, 2007. https://hdl.handle.net/10365/29851.
Texto completoDiaz, Carcamo Ricardo I. "Consumers acceptance of genetically modified food products in Chile and economic implications of invaisve [i.e. invasive] species in international trade". Online access for everyone, 2005. http://www.dissertations.wsu.edu/Thesis/Spring2005/r%5Fdiaz-carcamo%5F050305.pdf.
Texto completoFrost, Carolyn. "Covering the GMO issue : an overview for South African science reporters". Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52175.
Texto completoENGLISH ABSTRACT: The aim and function of this paper is to provide a balanced account of how the media, international and South African, have dealt with the issue of genetically modified organisms (GMOs). A selection of interviews, presentations, articles, transcripts and published reports forms the background of this interpretation, and offers insight into the history of the technology, the major role players, the legislation required and implemented, the question of environmental accountability, and the power of the media's influence. It addresses aspects of the causal relationship between the media and public understanding, and the subsequent power of the consumer as manifested by the perception of risk. The central theme of genetic engineering conjures up a variety of meanings and applications, and the plethora of available information is evaluated in an attempt to develop informed understanding for reporters covering the many dimensions of this development within the arena of science and technology.
AFRIKAANSE OPSOMMING: Die doel van hierdie verhandeling is om 'n ewewigtige oorsig te verstrek van hoe die media - Suid-Afrikaans sowel as internasionaal - die kwessie van geneties gemodifiseerde organismes gehanteer het. 'n Seleksie onderhoude, aanbiedinge, artikels, transkripsies, en gepubliseerde verslae vorm die basis van hierdie interpretasie, en verskaf 'n insig in die geskiedenis van die tegnologie, die belangrike rolspelers, nodige en géimplementeerde wetgewing, die vraag van omgewingstoerekenbaarheid, en die mag van die media se invloed. Dit spreek aspekte aan van die kousale verwantskap tussen die media en begrip deur die algemene publiek, en die daaropvolgende mag van die verbruiker, soos dit duidelik word in hulle insig in en begrip van die risiko-faktor. Die sentrale tema van genetiese modifisering bring te voorskyn 'n verskeidenheid betekenisse en aanwendings; en 'n oorsig van die massa beskikbare inligting word hier aangebied in 'n poging om aan verslaggewers ingeligte begrip aan te bied van die veelsydige omvang van die ontwikkeling van genetiese modifisering in die gebied van wetenskap en tegnologie.
Ruch, Alexander Martin. "Perceived organizational risks and reputations are related to individuals' decisions to eat genetically modified foods". Thesis, University of Iowa, 2016. https://ir.uiowa.edu/etd/6264.
Texto completoMoore, Elizabeth Louise. "Science, internationalization, and policy networks, regulating genetically-engineered food crops in Canada and the United States, 1973-1998". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ53851.pdf.
Texto completoMagnier, de Maisonneuve Alexandre. "The economics of regulatory standards : the case of GM thresholds in seed production /". free to MU campus, to others for purchase, 2003. http://wwwlib.umi.com/cr/mo/fullcit?p1418049.
Texto completoBerhaupt, Amanda. "The Perceptions, Attitudes and Practices of Registered Dietitians Regarding Functional Foods". FIU Digital Commons, 2010. http://digitalcommons.fiu.edu/etd/156.
Texto completoLi, Hanzhe. "Sentiment Analysis and Opinion Mining on Twitter with GMO Keyword". Thesis, North Dakota State University, 2016. http://hdl.handle.net/10365/25787.
Texto completoWaite, Aldious A. "Consumer Knowledge, Perception and Attitudes of Unlabeled Genetically Modified Foods of an Educated Population in the State of Kentucky". TopSCHOLAR®, 2017. https://digitalcommons.wku.edu/theses/2026.
Texto completoVermeulen, Hester. "Genetically modified white maize in South Africa : consumer perceptions and market segmentation". Diss., Pretoria : [s.n.], 2004. http://upetd.up.ac.za/thesis/available/etd-11222005-110807.
Texto completoRibeiro, Tatiane Godoy 1989. "A percepção do consumidor sobre o uso de organismos geneticamente modificados em alimentos". [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/254514.
Texto completoDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
Made available in DSpace on 2018-08-27T12:04:35Z (GMT). No. of bitstreams: 1 Ribeiro_TatianeGodoy_M.pdf: 2754332 bytes, checksum: 78f977788653152f817e74560614ee81 (MD5) Previous issue date: 2015
Resumo: O Brasil é um dos maiores produtores mundiais de alimentos transgênicos, porém faltam pesquisas que avaliem de forma mais profunda a opinião pública sobre o tema. Assim, o objetivo deste trabalho foi estudar a percepção do consumidor brasileiro sobre o uso de engenharia genética na produção de alimentos sob uma perspectiva de pesquisa quali-quantitativa. Inicialmente foram realizadas entrevistas com 48 consumidores na cidade de Campinas,SP, e os resultados mostraram que, de forma geral, há desconhecimento sobre os fundamentos científicos da biotecnologia moderna, bem como da sua finalidade na produção de alimentos. Os respondentes mostraram-se mais favoráveis à aplicação de engenharia genética aos produtos de origem vegetal e àqueles produzidos com uso de micro-organismos, em detrimento dos produtos de origem animal, notadamente a carne. Benefícios, tais como aumento de produtividade, melhoria de qualidade e possível redução do preço dos alimentos mostraram-se como motivadores do consumo. Por outro lado, há desconfiança justificada pelo risco de efeitos adversos à saúde e também ao meio ambiente, sobretudo em longo prazo. Três segmentos de consumidores foram identificados, quais sejam, os de atitude positiva em relação ao uso da engenharia genética na produção de alimentos, os de atitude negativa e os de atitude neutra a positiva. No segundo momento da pesquisa, avaliou-se a intenção de compra de alimentos produzidos por biotecnologia moderna por 55 consumidores combinando-se três fatores: tipo de alimento (de origem animal, vegetal ou produzido por processo microbiológico), denominação ("organismo geneticamente modificado" ou "transgênico") e benefícios (à saúde, ao meio ambiente ou nenhum benefício). A Análise de Conglomerados associada à Análise Conjunta demonstrou que, 1) produtos de origem animal são preferidos em comparação aos de origem vegetal ou produzidos por ação de micro-organismos, 2) a denominação "organismo geneticamente modificado" é preferida à "transgênico" e, finalmente, 3) benefícios à saúde ou ao meio ambiente são menos importantes e úteis na intenção de compra. Como conclusão, este estudo aponta a necessidade de estratégia de comunicação à população sobre os fundamentos, os possíveis benefícios e os riscos da engenharia genética de forma a promover o debate social e escolha consciente dos consumidores brasileiros em relação aos alimentos produzidos com tecnologias associadas
Abstract: Although Brazil is one of the world's largest producers of transgenic food, there are few studies assessing local public opinion on the subject. The aim of this work was to study the perception of Brazilian consumers about the use of genetic engineering on food production using a quali-quantitative research perspective. Firstly, 48 consumers were interviewed in the city Campinas, SP, and the results showed that, in general, lack of knowledge about the scientific fundamentals of modern biotechnology and its purposes on food production. The respondents showed to be more favorable to the application of genetic engineering on plants and and food produced by microbiological process, than on animal origin products, especially meat. Benefits, such as increased productivity, improved quality and possible price reduction appeared as the main drivers of consumption. On the other hand, it seems to be distrust justified by the risk of adverse effects on health and also to the environment, particularly in the long term. Three consumer clusters were identified: one showing positive attitude; another one with negative attitude; and a third group of consumer showing neutral to positive attitude towards the use of genetic engineering in food production. In the second part of the study, a conjoint study with 55 consumers was performed in order to assess the purchase intention of concepts of foods produced by modern biotechnology. Concepts were defined by combining three factors: type of food (animal, plant or produced by microbiological process), name ("genetically modified organism" or "transgenic") and benefits (to the health, to the environment or no benefit stated). Cluster Analysis associated with Conjoint Analysis showed that: 1) animal products are preferred in comparison to plants or food produced by microbiological process, 2) the name "genetically modified organism" is preferred to the "transgenic" and finally, 3) benefits to health or to the environment are less important in the purchase intention. In conclusion, this study indicates the need of a communication strategy to inform the Brazilian population about the fundamentals and the possible benefits and risks of genetic engineering in order to promote a social debate and more conscious choice by local consumers regarding food produced with associated technologies
Mestrado
Consumo e Qualidade de Alimentos
Mestra em Alimentos e Nutrição
Денисенко, Анатолій Федорович, Анатолий Федорович Денисенко, Anatolii Fedorovych Denysenko, Олександра Олександрівна Білопільська, Александра Александровна Белопольская y Oleksandra Oleksandrivna Bilopilska. "Аналіз проблеми виробництва та споживання генитично модифікованих продуктів в Україні". Thesis, Вид-во СумДУ, 2010. http://essuir.sumdu.edu.ua/handle/123456789/5819.
Texto completoЯхненко, Олена Миколаївна, Елена Николаевна Яхненко, Olena Mykolaivna Yakhnenko y С. Ю. Шоломий. "ГМО в продуктах питания". Thesis, Изд-во СумГУ, 2010. http://essuir.sumdu.edu.ua/handle/123456789/5799.
Texto completoKonduru, Srinivasa Prasad Kalaitzandonakes Nicholas G. "Three essays on the potential economic impacts of biotech crops in the presence of asynchronous regulatory approval". Diss., Columbia, Mo. : University of Missouri--Columbia, 2008. http://hdl.handle.net/10355/6642.
Texto completoLu, Linqi. "Framing Genetically-modified Foods Communication in China: A Content Analysis of News Coverage of People’s Daily and Southern Metropolis". Scholar Commons, 2017. http://scholarcommons.usf.edu/etd/7054.
Texto completoShakya, Sumadhur. "Valuing and Pricing of Random and Non-Persistent Genetically Modified Traits (Corn and HRSW)". Thesis, North Dakota State University, 2009. https://hdl.handle.net/10365/29713.
Texto completoVogliano, Christopher T. "Knowledge Base and Perception Registered Dietitians Hold on the Genetic Modification of Foods". Kent State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=kent1353297827.
Texto completoBurchardi, Jan-Erik. "Die Vereinbarkeit der europäischen Vorschriften zur Kennzeichnung gentechnisch veränderter Lebensmittel mit dem Welthandelsrecht /". Berlin : Duncker und Humblot, 2007. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=015744372&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Texto completoMiguel, Fernando Bergantini [UNESP]. "Rentabilidade e risco da produção de milho safrinha geneticamente modificado na região de Guaíra-SP". Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/101955.
Texto completoUma das razões para a rápida adoção de milho transgênico no Brasil foi o benefício econômico que essa tecnologia pode proporcionar aos produtores, como redução nos custos de aplicação de inseticidas e aumento da renda, através da redução de perda de produtividade por ataques de insetos. Esses benefícios superam os custos da adoção de tal tecnologia, que é o valor (R$) pago pelos produtores ao usar a semente de milho geneticamente modificada (GM). Foi objetivo deste estudo dimensionar o retorno econômico e os riscos para os adotantes de milho GM em uma das principais regiões produtoras do Estado de São Paulo (Guaíra/SP), a partir da análise de variação das quantidades e preços de inseticidas 10 utilizados, ganhos em produtividade e variação das diferenças de preços de sementes de milho GM em relação aos híbridos convencionais, levando-se em consideração a variação dos preços de milho durante o período de estudo. A metodologia utilizada foi a de avaliação dos benefícios líquidos, ou seja, os ganhos econômicos menos os custos da tecnologia transgênica, sob condições de risco. Os benefícios líquidos podem oscilar em função de quatro variáveis críticas: (1) produtividade do milho transgênico; (2) custos de controle de lagartas; (3) preço do milho; (4) custo de sementes transgênicas. As funções de distribuição de probabilidade dessas variáveis críticas foram estimadas e incluídas na equação de benefícios líquidos. Utilizando o método de simulação de Monte Carlo, foram estimados os seguintes conjuntos de indicadores: medidas de tendência central, variabilidade dos benefícios líquidos (benefícios totais menos custos totais), análise de sensibilidade dos benefícios líquidos em relação às variáveis críticas e, por fim, mapeamento de risco dos adotantes da tecnologia transgênica. Esses indicadores permitiram desenhar cenários econômico...
One of the reasons for the rapid adoption of transgenic corn in Brazil was the economic benefit this technology can provide to the producers, such as a reduction in the costs of applying insecticides and increased income due to the reduction in productivity losses caused by insect attacks. These benefits outweigh the costs of adopting such technology, which is the value (R$) paid by the farmers to use genetically modified (GM) seeds. The objective of this study was to dimension the economic risks and returns to the adopters of GM corn in one of the major corn producing regions of the state of São Paulo, as from the analysis of variation of the quantities and prices of insecticides used, 12 productivity gains and variation in the price differentials of the GM corn seeds in relation to conventional hybrids, taking into account the variation in corn prices during the period studied. The methodology used was the analysis of the net benefits, that is, the economic gains minus the costs of GM technology under conditions of risk. Net benefits can fluctuate as a function of four critical variables: 1) GM corn productivity; 2) costs of caterpillar control; 3) the price of corn; 4) the cost of GM seeds. The probability distribution functions of these critical variables were estimated and included in the net benefit equation. Using the Monte Carlo simulation methodology, the following indicator sets were estimated: central tendency measurements, variability in net benefits (total benefits minus total costs), sensitivity analysis of the net benefits in relation to the critical variables, and finally, the mapping of the risk to GM technology adopters. These indicators allow one to design economic scenarios associated with their probability of occurring. In general, it appeared that farmers who adopted the planting of transgenic corn showed positive profitability levels. The probability of positive ...
Miguel, Fernando Bergantini 1964. "Rentabilidade e risco da produção de milho safrinha geneticamente modificado na região de Guaíra-SP /". Botucatu, 2013. http://hdl.handle.net/11449/101955.
Texto completoBanca: Luiz Cesar Ribas
Banca: Carmen Ozana de Melo
Banca: Ivana Marinho Barbaro
Banca: Izabel Cristina Takitane
Resumo: Uma das razões para a rápida adoção de milho transgênico no Brasil foi o benefício econômico que essa tecnologia pode proporcionar aos produtores, como redução nos custos de aplicação de inseticidas e aumento da renda, através da redução de perda de produtividade por ataques de insetos. Esses benefícios superam os custos da adoção de tal tecnologia, que é o valor (R$) pago pelos produtores ao usar a semente de milho geneticamente modificada (GM). Foi objetivo deste estudo dimensionar o retorno econômico e os riscos para os adotantes de milho GM em uma das principais regiões produtoras do Estado de São Paulo (Guaíra/SP), a partir da análise de variação das quantidades e preços de inseticidas 10 utilizados, ganhos em produtividade e variação das diferenças de preços de sementes de milho GM em relação aos híbridos convencionais, levando-se em consideração a variação dos preços de milho durante o período de estudo. A metodologia utilizada foi a de avaliação dos benefícios líquidos, ou seja, os ganhos econômicos menos os custos da tecnologia transgênica, sob condições de risco. Os benefícios líquidos podem oscilar em função de quatro variáveis críticas: (1) produtividade do milho transgênico; (2) custos de controle de lagartas; (3) preço do milho; (4) custo de sementes transgênicas. As funções de distribuição de probabilidade dessas variáveis críticas foram estimadas e incluídas na equação de benefícios líquidos. Utilizando o método de simulação de Monte Carlo, foram estimados os seguintes conjuntos de indicadores: medidas de tendência central, variabilidade dos benefícios líquidos (benefícios totais menos custos totais), análise de sensibilidade dos benefícios líquidos em relação às variáveis críticas e, por fim, mapeamento de risco dos adotantes da tecnologia transgênica. Esses indicadores permitiram desenhar cenários econômico ...
Abstract: One of the reasons for the rapid adoption of transgenic corn in Brazil was the economic benefit this technology can provide to the producers, such as a reduction in the costs of applying insecticides and increased income due to the reduction in productivity losses caused by insect attacks. These benefits outweigh the costs of adopting such technology, which is the value (R$) paid by the farmers to use genetically modified (GM) seeds. The objective of this study was to dimension the economic risks and returns to the adopters of GM corn in one of the major corn producing regions of the state of São Paulo, as from the analysis of variation of the quantities and prices of insecticides used, 12 productivity gains and variation in the price differentials of the GM corn seeds in relation to conventional hybrids, taking into account the variation in corn prices during the period studied. The methodology used was the analysis of the net benefits, that is, the economic gains minus the costs of GM technology under conditions of risk. Net benefits can fluctuate as a function of four critical variables: 1) GM corn productivity; 2) costs of caterpillar control; 3) the price of corn; 4) the cost of GM seeds. The probability distribution functions of these critical variables were estimated and included in the net benefit equation. Using the Monte Carlo simulation methodology, the following indicator sets were estimated: central tendency measurements, variability in net benefits (total benefits minus total costs), sensitivity analysis of the net benefits in relation to the critical variables, and finally, the mapping of the risk to GM technology adopters. These indicators allow one to design economic scenarios associated with their probability of occurring. In general, it appeared that farmers who adopted the planting of transgenic corn showed positive profitability levels. The probability of positive ...
Doutor
Flagg, Ian Marshall. "The Valuation of Agricultural Biotechnology: The Real Options Approach". Thesis, North Dakota State University, 2008. https://hdl.handle.net/10365/29761.
Texto completoAddey, Kwame Asiam. "The Role of Trade Facilitation Indicators and Genetically Engineered Restrictive Index on U.S. State Exports and Efficiency". Thesis, North Dakota State University, 2018. https://hdl.handle.net/10365/29005.
Texto completoМузика, І. y В. Слободенюк. "Сучасні погляди щодо безпечності генномодифікованої продукції для здоров'я населення". Thesis, Сумський державний університет, 2016. http://essuir.sumdu.edu.ua/handle/123456789/45183.
Texto completoSchneider, Patrícia Maria. "Segurança alimentar e princípio da informação sobre alimentos geneticamente modificados : perspectivas jurídica e socioambiental". reponame:Repositório Institucional da UCS, 2010. https://repositorio.ucs.br/handle/11338/526.
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The social and environmental views are closely related, which attracts the socioenvironmental perspective. All artifacts in the living conditions of the population is comprised of biotechnology in environmental protection. The commercialization of genetically modified organisms in food generates much debate and scientific doctrine today. However, it is possible to draw some myths and commit some observations on the subject. Myths bring the promises of seeking satisfying hunger by increasing production with the use of biotechnology in the food, as well as the priority in food production with properties beneficial to health. The findings run into some negative episodes involving the use of genetically modified organisms and also strong in the domain of large corporations that are dictating the rules of biotechnology development. This picture, in the marketing of food, the availability of information through labeling is emphasized, it is important to consider the legal treatment that has been given at the domestic, European Union, from the perspective of the United States and the Codex Alimentarius. Given the legal context, it remains clear that there are still many controversies and the availability of information is still some challenges, particularly in reviewing the labeling and reconciliation of information about cultural diversity. For information to have full effect should be inter-related education and participation of stakeholders.
Puta, Usanda. "Effects of genetically modified maize (MON810) and its residues on the functional diversity of microorganisms in two South African soils". Thesis, University of Fort Hare, 2011. http://hdl.handle.net/10353/419.
Texto completoKotey, Daniel Ashie. "Genetically modified (GM) maize cultivation by smallholders in the Eastern Cape Province of South Africa: Effects on target and non-target organisms and adoption challenges". Thesis, University of Fort Hare, 2017. http://hdl.handle.net/10353/5161.
Texto completoRenton, Michelle Susan. "Influencing consumer perceptions of a social issue: an experiment on the effects of credibility of the source, message sidedness and inward/outward focus on consumer attitudes toward genetically modified foods". Thesis, University of Canterbury. Management, 2008. http://hdl.handle.net/10092/874.
Texto completoLieberman, Sarah Zilda. "An analysis of the internal and external repercussions of the European Union’s ’de facto’ moratorium on the authorisation of new genetically modified crops and foods". Thesis, University of Newcastle Upon Tyne, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.437975.
Texto completoFörster, Susanne. "Internationale Haftungsregeln für schädliche Folgewirkungen gentechnisch veränderter Organismen : europäische und internationale Entwicklungen und Eckwerte für ein Haftungsregime im internationalen Recht /". Berlin : Springer, 2007. http://www.netlibrary.com/urlapi.asp?action=summary&v=1&bookid=197111.
Texto completoEnglish summary: International liability for damage caused by genetically modified organisms. Copyright by Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., to be exercised by Max-Planck-Institut für ausländisches öffentliches Recht und Völkerrecht, Heidelberg. Description based on print version record. Includes bibliographical references (p. [393]-410) and index.
Du, Plessis Marthinus Johannes. "The international political economy of the Cartagena Protocol on biosafety". Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52543.
Texto completoENGLISH ABSTRACT: The development of the global biotechnology industry largely coincided with the development of the US biotechnology industry. This resulted in this industry's oligopolistic and centralised nature where only a few multinational chemical and pharmaceutical companies control most biotechnology processes and production of commodities emanating from these processes. The governance of biotechnology has, until recently, been dominated by state actors who have endeavoured to secure national interests, including those of large multinational corporations (MNCs) based within their boundaries. The technological ability of developed states to exploit and use unevenly distributed resources to their advantage means that an uneven relationship exists between these and poor developing countries. This has been highlighted by differences in public opinion about the role and application of biotechnology in society. While some opinions favour the use and application of biotechnology to enhance food supplies and boost production levels and trade, other opinions caution against the possible hazards that genetically manipulated organisms (GMOs) hold for the environment and human existence. The commercialisation of biotechnology has resulted in the exponential growth of genetically manipulated crops in especially the United States and countries like Argentina and Canada. These countries produce large surpluses of staple grains such as corn and soya and try to sell these to countries with food supply problems. The clash in commercial interests stemming from developed countries' insistence on the protection of intellectual property rights (IPR) on genetically manipulated (GM) seeds has caused considerable conflict with poor farmers who will not be able to sustain their livelihoods if they cannot save seeds for future harvests. This is one aspect of the problems surrounding the protection of knowledge products that is exacerbated by the scientific uncertainty pertaining to the risk involved with biotechnology. While some observers agitate for precaution with the use of GMOs, others feel that a lack of scientific proof of harm is sufficient grounds for proceeding with developments in biotechnology. Conversely, there are some that feel that biotechnology is market driven instead of human needs driven, ultimately resulting in developing countries receiving very little benefit from it. The Cartagena Protocol on biosafety was drafted to address some of the difficulties involved with the transboundary movement of GMOs. Although it holds very specific advantages for developing countries, as a regulatory framework it is limited in its scope and application. Developing countries are limited in their policy options to address their need to protect biodiversity and secure their food supply. This means that considerable challenges and constraints await these countries in utilising global governance of public goods and building their human and technological capacities.
AFRIKAANSE OPSOMMING: Die ontwikkeling van die globale biotegnologie-industrie het grootliks saamgeval met die ontwikkeling van die Verenigde State se biotegnologie-industrie. Dit het aanleiding gegee tot hierdie industrie se oligopolistiese en gesentraliseerde aard waar slegs enkele multinasionale chemiese en farmaseutiese maatskappye die meeste biotegnologie prosesse en die vervaardiging van kommoditeite uit daardie prosesse beheer. Die regering van biotegnologie was tot onlangs oorheers deur staatsakteurs wie gepoog het om nasionale belange te beskerm, insluitend die belange van multinasionale korporasies (MNK) wat vanuit hulle grondgebied funksioneer. Die tegnologiese vermoë van ontwikkelde state om oneweredig verspreide hulpbronne tot eie gewin te benut beteken dat 'n ongelyke verhouding bestaan tussen hierdie en arm ontwikkelende state. Dit word beklemtoon deur verskille in openbare mening oor die rol en aanwending van biotegnologie in die samelewing. Terwyl sekere opinies ten gunste van die aanwending van biotegnologie vir die verbetering van voedselbronne en produksievlakke en handel is, dui ander opinies op die moontlike gevare wat geneties gemanipuleerde organismes (GMOs) vir die omgewing en menslike voortbestaan inhou. Die kommersialisering van biotegnologie het gelei tot die eksponensiële groei van geneties gemanipuleerde gewasse in veral die Verenigde State en state soos Argentinië en Kanada. Hierdie state produseer groot hoeveelhede stapelgrane soos mielies en soja en poog om dit te verkoop aan state met voedselvoorsieningsprobleme. Die botsing in kommersiële belange wat spruit uit ontwikkelde state se aandrang op die beskerming van intellektuele eiendomsreg op geneties gemanipuleerde saad veroorsaak beduidende konflik met arm landbouers wie nie hulle lewensonderhoud kan verseker as hulle nie saad kan berg vir toekomstige saaiseisoene nie. Dit is een aspek van die problematiek rondom die beskerming van kennisprodukte wat vererger word deur die wetenskaplike onsekerheid wat gepaard gaan met die risiko's van biotegnologie. Terwyl sekere waarnemers vir waaksaamheid pleit in die gebruik van GMOs, is daar ander wat voel dat 'n gebrek aan wetenskaplike bewyse van skade genoegsame gronde is vir die voortsetting van ontwikkelings in biotegnologie. Insgelyks is daar diegene wat meen dat biotegnologie markgedrewe in plaas van menslike behoefte gedrewe is, wat uiteindelik daartoe lei dat ontwikkelende state baie min voordeel daaruit trek. Die Kartagena Protokoloor bioveiligheid is opgestel om van die probleme betrokke by die oorgrens verskuiwing van GMOs aan te spreek. Hoewel dit spesifieke voordele vir ontikkelende state inhou is dit as reguleringsraamwerk beperk in omvang en aanwending. Ontwikkelende state het beperkte beleidsopsies om hulle behoefte om biodiversiteit te beskerm en voedselvoorsiening te verseker, aan te spreek. Dit beteken dat beduidende uitdagings en beperkings hierdie state in die benutting van globale regering van openbare goedere vir die bou van menslike en tegnologiese kapasiteite in die gesig staar.
Conradie, Tobie Tertius. "Genetic engineering of sugarcane for increased sucrose and consumer acceptance". Thesis, Stellenbosch : Stellenbosch University, 2011. http://hdl.handle.net/10019.1/17826.
Texto completoENGLISH ABSTRACT: Sugarcane is a crop that is farmed commercially due to the high amounts of sucrose that is stored within the mature internodes of the stem. Numerous studies have been done to understand sugar metabolism in this crop as well as to enhance sucrose yields. Until now sugarcane improvement strategies have been implemented through either breeding programs or transgenic manipulation. Public mistrust and regulatory hurdles, however, have made the commercialisation of transgenic crops difficult, expensive and timeconsuming. In this thesis two projects will address issues relating to the above. The first will address an effort to increase sucrose accumulation within the sugarcane culm. This was attempted via the expression of an Arabidopsis thaliana vacuolar pyrophosphatase (AtV-PPase) gene, linked to the maize ubiquitin promoter, in sugarcane callus. It was anticipated that increased activity of the tonoplast-bound AtV-PPase will result in increased sucrose accumulation in the vacuole. Transgenic sugarcane callus lines were tested for soluble sugar content which suggested no significant increase in sucrose content. However, this may change upon further assessment of sugarcane suspension cultures and glasshouse plants. The second project was concerned with the development of a novel sugarcane transformation technology that utilises only sugarcane sequences. This ‘cisgenic’ approach to sugarcane transformation will require a native sugarcane promoter, terminator, vector backbone and selection marker. It was attempted to first isolate a functional promoter as well as developing a selection system based on an endogenous selection marker. A promoter was amplified from sugarcane, using primers designed on a sorghum template, and its expression assessed using a GFP reporter gene. Unfortunately expression could not be confirmed in transgenic sugarcane callus. Currently, an alternative approach is followed by using short fragments of constitutively expressed genes to screen sugarcane Bacterial Artificial Chromosome (BAC) libraries to isolate their corresponding promoters. Lastly, it was attempted to develop a selection system for transgenic sugarcane based on resistance to the herbicide chlorosulfuron. A mutant acetolactate synthase (alsb) gene from tobacco, which has shown to confer resistance to the tobacco, was transformed into sugarcane callus. It was anticipated that this gene will confer chlorosulfuron resistance to transgenic sugarcane. If resistance is achieved, the corresponding sugarcane gene will be mutated via site-directed mutagenesis and checked if it also confers resistance to sugarcane. Results showed that although transgenic lines were generated, resistance development is still inconclusive.
AFRIKAANSE OPSOMMING: Suikerriet is ‘n kommersiële gewas wat verbou word as gevolg van die hoë hoeveelhede sukrose wat gestoor word in die volwasse tussenknope van die stam. Verskeie studies is al gedoen om suiker metabolisme in die gewas te ondersoek, sowel as om die sukrose opbrengs te verhoog. Huidige strategieë vir suikerriet verbetering word beywer deur middel van teel-programme of transgeniese manipulasie. Die kommersialiseëring van transgeniese gewasse word egter bemoeilik deur publieke wanpersepsies, sowel as regulatoriese uitdagings. Hierdie tesis beoog om boenoemde kwessies aan te spreek, deur middel van twee projekte. Die eerste projek poog om sukrose akkumulasie in sukerriet te verhoog. Dit was onderneem om die Arabidopsis thaliana vakuolere pirofosfatase (AtV-PPase) geen, wat verbind is met die mielie ubiquitien promoter, uit te druk in suikerriet kallus. Daar was verwag dat die verhoogde aktiwiteit van die tonoplast-gebonde AtV-PPase sal veroorsaak dat meer sukrose in die vakuool akkumuleer. Oplosbare suiker inhoud was getoets in transgeniese suikerriet kallus lyne, maar geen merkbare verhoging in sukrose inhoud was waargeneem nie. Hierdie mag egter verander met verdere ondersoeke in suikerriet suspensie-kulture en glashuis-plante. Die tweede projek het beywer om ‘n nuwe suikerriet transformasie tegnologie te ontwikkel, wat slegs van suikerriet genetiese materiaal gebruik maak. Hierdie ‘cisgeniese’ benadering tot suikerriet transformasie sal ‘n inheemse suikerriet promoter, terminator, vektor ruggraat en seleksie-merker, benodig. Dit was eers beoog om ‘n funksionele promoter te isoleer, sowel as om ‘n seleksie sisteem, gebasseer op ‘n inheemse seleksie merker, te ontwikkel. Deur gebruik te maak van primers wat op ‘n sorghum templaat gebasseer is, was ‘n promotor geisoleer vanuit suikerriet; die uitdrukking hiervan is bepaal deur gebruik te maak van ‘n GFP verklikker geen. Ongelukkig kon uitdrukking nie bevestig word in transgeniese suikerriet kallus nie. Tans word suikerriet Kunsmatige Bakterieële Chromosoom (KBC) biblioteke geskandeer, deur gebruik te maak van geen-fragmente van globaal-uitgedrukte gene, om ooreenstemmende suikerriet promoters te isoleer. Die tweede deel van die cisgeniese projek het beoog om ‘n seleksie sisteem vir transgeniese suikerriet te ontwikkel, wat gebasseer is op weerstand teen die plantdoder chlorosulfuron. Suikerriet kallus was getranformeer met ‘n mutante tabak geen – asektolaktaat sintase (alsb) – wat chlorosulfuron weerstand in tabak meebring. Daar was verwag dat die geen chlorosulfuron weerstand aan transgeniese suikerriet sou oordra. Indien weerstand ontwikkel, sal die ooreenstemende suikerriet geen deur gerigte mutagenese gemuteer word; dan sal dit kan bepaal word of weerstand ook oorgedra word aan suikerriet. Daar is bevind dat alhoewel transgeniese lyne gegenereer is, daar steeds nie ‘n konklusiewe bevestiging van weerstand ontwikkeling is nie.
Santos-Donado, Priscila Robertina dos. "Estudo proteômico de variedades de milho (Zea mays L.) obtidas por melhoramento clássico e por recombinação genética". Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/9/9131/tde-26012017-141922/.
Texto completoThe classic genetic breeding of corn seeds (Zea mays) has enabled the development of many varieties, including corn with improved protein quality (Quality Protein Maize, QPM), which aimed to increase protein levels and nutritional properties. On the other hand, new commercial varieties have been obtained out of genetically modified (GM) vegetables, with a focus in agronomic parameters. In both cases, the safety of these varieties for food use is one of the main concerns for the developers and for the regulatory agencies. Substantial Equivalence is the basis of the safety evaluation system for genetically modified crops, however, alterations in the protein expressions are not been properly analyzed and clarified. The protein approaches complement the techniques of biosafety evaluation for GM foods, as well as allow for possible undesirable effects derived from classic improvement to be investigated. The goals of the current studies were to characterize and compare the protein profiles of the different varieties of conventionally improved (QPM) and genetically modified (GM) corn, against their respective conventional lines using proteomic techniques, such as, two-dimensional electrophoresis (2-DE), bottom up shotgun (gel-free) and masses spectrometry (MS). In a first instance of the study, three samples of corn were used, two of conventional varieties with QPM (QP1 and QP2) and one conventional normal variety (CN). In a second instance of the study, two cultures of GM corn (GM1 and GM2) were analyzed and their respective conventional genitors (CG1 and CG2). The chemical compositions of all the samples were also evaluated for their Substantial Equivalence. The protein raw extract was submitted to analysis of one-dimensional (1-DE), two-dimensional (2-DE) electrophoresis, and bottom up shotgun (gel-free). The protein image maps were analyzed by the Image Master 2D Platinum 7.0 (GE) software. The spots which were expressed and selected differentially were sequenced by MS. By the chemical composition of the main fractions of the samples of corn, it was possible to identify the substantial equivalence between the conventional samples and GMs, likewise with OPMs and their conventional in the ranges of variability which were expected for the species. On the 1-DE gel, it was observed protein bands with similar profiles amongst the groups of evaluated samples for both studies. In the images of the 2-DE gel, there were no alterations between the GM corn and their respective conventional genitors (CGs), but only differences in intensity of the protein spots. The OPM and CN varieties presented differences due to the distribution of the spots. The protein maps of samples CG1 vs. GM1 and CG2 vs. GM2 presented greater similarities with the percentages of matchings superior to 70%, while the percentage of matchings among different varieties (QPMs and CN) were smaller. In total, there were 219 proteins identified in the samples CGs vs. GMs and QPMs vs. CN, classified by the biologic processes and molecular function. In conclusion, there were found differences between the cultures of GMs and CGs, indicating a normal variation among the corn varieties, which do not affect the food security of the studied samples. As per the samples with QPM and CN, the differences found were due to the line distances or germplasm.
"Detection of genetically modified foods (GMFs)". 2001. http://library.cuhk.edu.hk/record=b5896000.
Texto completoThesis (M.Phil.)--Chinese University of Hong Kong, 2001.
Includes bibliographical references (leaves 175-192).
Abstracts in English and Chinese.
Declaration --- p.ii
Acknowledgements --- p.iii
Abstract --- p.iv
Abbreviation --- p.vi
Table of Contents --- p.vii
Chapter Chapter 1 --- Introduction --- p.1
Chapter Section I --- The Making of Genetically Modified Organisms --- p.2
Chapter 1.1 --- Conventional breeding in agriculture --- p.2
Chapter 1.2 --- What is genetic engineering? --- p.4
Chapter 1.3 --- Plant transformation --- p.5
Chapter 1.3.1 --- Agrobacterium-mediated --- p.6
Chapter 1.3.2 --- Direct gene transfer --- p.8
Chapter 1.3.2.1 --- Microparticle bombardment --- p.8
Chapter 1.3.2.2 --- Protoplasts --- p.9
Chapter 1.3.3 --- Gene silencing --- p.10
Chapter 1.4 --- Examples of genetically modified crops --- p.13
Chapter 1.5 --- Foreign genes commonly found in transgenic plants --- p.14
Chapter Section II --- Benefits and Environmental Concern of GMOs --- p.17
Chapter 2.1 --- Mechanism of GMO --- p.17
Chapter 2.1.1 --- Herbicide tolerant crops --- p.18
Chapter 2.1.2 --- Insect resistant crops --- p.19
Chapter 2.1.3 --- Delayed ripening crops --- p.20
Chapter 2.1.4 --- Virus resistant crops --- p.20
Chapter 2.2 --- Benefits of GMOs --- p.21
Chapter 2.3 --- Impact of GM foods to human health and the environment --- p.22
Chapter 2.3.1 --- Human health --- p.22
Chapter 2.3.1.1 --- GM potatoes --- p.23
Chapter 2.3.1.2 --- CaMV risks? --- p.24
Chapter 2.3.1.3 --- Food allergy --- p.25
Chapter 2.3.2 --- Environmental concerns --- p.26
Chapter 2.3.2.1 --- Horizontal gene transfer --- p.27
Chapter 2.3.2.1.1 --- Selectable marker genes --- p.27
Chapter 2.3.2.1.2 --- Herbicide resistant genes --- p.29
Chapter 2.3.2.1.3 --- Insect resistant genes --- p.29
Chapter 2.3.2.2 --- Ecology --- p.30
Chapter 2.3.2.2.1 --- Monarch butterfly --- p.30
Chapter Section III --- Future developments of GMO --- p.32
Chapter 3.1 --- Designer Food and engineered plants --- p.32
Chapter 3.1.1 --- Insect resistance --- p.33
Chapter 3.1.2 --- Viral resistance --- p.33
Chapter 3.1.3 --- Fungal resistance --- p.34
Chapter 3.1.4 --- Nutritional quality --- p.34
Chapter 3.1.5 --- Modifications of oil composition --- p.35
Chapter 3.1.6 --- Medical applications --- p.37
Chapter 3.1.7 --- Environmental applications --- p.40
Chapter 3.1.7.1 --- Tolerance to high salinity and drought --- p.40
Chapter 3.1.7.2 --- Tolerance to frost --- p.41
Chapter 3.1.7.3 --- Bioremediation --- p.42
Chapter 3.1.7.4 --- Biodegradable products --- p.43
Chapter Section IV --- Regulation of GMO --- p.44
Chapter 4.1 --- The question of labeling --- p.44
Chapter 4.1.1 --- Moral and ethical issues --- p.44
Chapter 4.1.2 --- Animal welfare --- p.45
Chapter 4.2 --- International practice in GMO labeling --- p.46
Chapter 4.2.1 --- United States of America --- p.46
Chapter 4.2.2 --- Canada --- p.48
Chapter 4.2.3 --- European Union --- p.49
Chapter 4.2.4 --- Australia and New Zealand --- p.50
Chapter 4.2.5 --- Japan --- p.51
Chapter 4.2.6 --- Republic of Korea --- p.52
Chapter 4.2.7 --- China --- p.53
Chapter 4.2.8 --- Taiwan --- p.53
Chapter 4.2.9 --- Hong Kong --- p.54
Chapter Section V --- Uses of crops --- p.56
Chapter 5.1 --- Uses of crops --- p.56
Chapter 5.1.1 --- Soybean --- p.56
Chapter 5.1.2 --- Corn --- p.57
Chapter 5.1.3 --- Tomato --- p.58
Chapter 5.1.4 --- Potato --- p.59
Chapter 5.1.5 --- Rice --- p.60
Chapter 5.1.6 --- Rapeseed --- p.61
Chapter 5.1.7 --- Oil --- p.62
Chapter 5.2 --- "Food additives, hormones and flavourings" --- p.63
Chapter Chapter 2 --- Materials & Methods --- p.65
Chapter 2.1 --- Materials --- p.66
Chapter 2.1.1 --- Growth media & agar --- p.66
Chapter 2.1.2 --- Reagents for agarose gel electrophoresis --- p.67
Chapter 2.1.3 --- Reagents for preparation of competent cells --- p.67
Chapter 2.1.4 --- Reagents for measurement of DNA concentration --- p.68
Chapter 2.1.4.1 --- Measurement of DNA concentration by PicoGreen --- p.68
Chapter 2.1.5 --- Reagents for Southern hybridization --- p.68
Chapter 2.2 --- Methods --- p.70
Chapter 2.2.1 --- Restriction endonuclease digestion --- p.70
Chapter 2.2.2 --- Agarose gel electrophoresis of DNA --- p.70
Chapter 2.2.3 --- DNA recovery from agarose gel --- p.71
Chapter 2.2.3.1 --- QIAquick® gel extraction --- p.71
Chapter 2.2.4 --- Ligation of purified DNA fragment into vector --- p.72
Chapter 2.2.5 --- Transformation --- p.72
Chapter 2.2.6 --- Rubidium chloride method for making competent cells --- p.12
Chapter 2.2.7 --- Plasmid DNA preparation --- p.73
Chapter 2.2.7.1 --- Concert Rapid Mini Prep --- p.73
Chapter 2.2.7.2 --- QIAprep® Miniprep --- p.74
Chapter 2.2.8 --- Extraction of plant genomic DNA --- p.75
Chapter 2.2.8.1 --- Qiagen DNeasy´ёØ Plant Mini Kit --- p.75
Chapter 2.2.9 --- Southern Hybridization --- p.75
Chapter 2.2.9.1 --- Denaturation --- p.76
Chapter 2.2.9.2 --- Blot transfer --- p.76
Chapter 2.2.9.3 --- Pre-hybridization --- p.77
Chapter 2.2.9.4 --- Synthesis of radiolabelled probe --- p.77
Chapter 2.2.9.5 --- Hybridization of radiolabelled probe on filter --- p.77
Chapter 2.2.9.6. --- Detection of hybridized probes --- p.78
Chapter 2.2.10 --- Measurement of DNA concentration --- p.78
Chapter 2.2.10.1 --- Determination of DNA on EtBr stained gel --- p.78
Chapter 2.2.10.2 --- Determination of DNA by UV spectrophotometer --- p.78
Chapter 2.2.10.3 --- Determination of DNA by PicoGreen --- p.79
Chapter 2.2.11 --- DNA sequencing --- p.80
Chapter 2.2.11.1 --- Automated sequencing by ABI Prism 377 --- p.80
Chapter Chapter 3 --- PCR Diagnostics --- p.81
Chapter 3.1 --- Applications of PCR to processed foods --- p.82
Chapter 3.1.1 --- DNA quality --- p.82
Chapter 3.1.2 --- PCR & Multiplex PCR --- p.83
Chapter 3.1.3 --- Choice of primers --- p.84
Chapter 3.1.4 --- Inhibitors --- p.84
Chapter 3.2 --- Materials & Methods --- p.85
Chapter 3.2.1 --- Selection of primers --- p.85
Chapter 3.2.2 --- Amplification of target sequences --- p.86
Chapter 3.2.3 --- Multiple amplification of target sequences --- p.87
Chapter 3.3 --- Results --- p.88
Chapter 3.4 --- Discussion --- p.93
Chapter Chapter 4 --- Quality Control in GMO detection --- p.95
Chapter 4.1 --- Standardization of pre- and post- PCR analysis --- p.96
Chapter 4.1.1 --- General guidelines --- p.96
Chapter 4.1.2 --- UV irradiation --- p.97
Chapter 4.1.3 --- Inactivation protocols --- p.93
Chapter 4.1.4 --- Positive and negative controls --- p.99
Chapter 4.1.5 --- PCR verification --- p.99
Chapter 4.1.6 --- Equipment decontamination --- p.100
Chapter 4.2 --- Materials & Methods --- p.101
Chapter 4.2.1 --- Selection of primers for external control --- p.101
Chapter 4.2.2 --- Development of the external control --- p.101
Chapter 4.2.3 --- Selection of primers for internal control --- p.103
Chapter 4.3 --- Results --- p.104
Chapter 4.4 --- Discussion --- p.107
Chapter Chapter 5 --- DNA extraction from food samples --- p.110
Chapter 5.1 --- Introduction --- p.111
Chapter 5.2 --- Reagents and Buffers for DNA extraction from food samples --- p.112
Chapter 5.2.1 --- Cetyltrimethylammonium bromide (CTAB) extraction method --- p.112
Chapter 5.2.2 --- Organic-based extraction method --- p.113
Chapter 5.2.3 --- Potassium acetate/sodium dodecyl sulphate precipitation method --- p.113
Chapter 5.2.4 --- Hexane-based extraction method --- p.114
Chapter 5.3 --- Weight and names of samples --- p.115
Chapter 5.4 --- DNA extraction methods --- p.115
Chapter 5.4.1 --- CTAB extraction method --- p.115
Chapter 5.4.2 --- Qiagen DNeasy´ёØ plant mini kit --- p.116
Chapter 5.4.3 --- Promega Wizard® genomic DNA purification --- p.116
Chapter 5.4.4 --- Promega Wizard® Magnetic DNA purification system --- p.117
Chapter 5.4.5 --- Promega Wizard® DNA Clean-Up system --- p.118
Chapter 5.4.6 --- Qiagen QIAshreddrer´ёØ and QIAamp spin column --- p.119
Chapter 5.4.7 --- Chelex-based extraction method --- p.119
Chapter 5.4.8 --- Organic-based extraction method --- p.120
Chapter 5.4.9 --- Nucleon PhytoPure extraction and purification method --- p.120
Chapter 5.4.10 --- Potassium acetate/SDS precipitation method --- p.121
Chapter 5.4.11 --- Hexane-based extraction method --- p.122
Chapter 5.5 --- Results --- p.123
Chapter 5.5.1 --- Comparison of eleven extraction methods --- p.123
Chapter 5.5.2 --- Comparison of DNA extraction on selected methods --- p.125
Chapter 5.6 --- Discussion --- p.132
Chapter Chapter 6 --- Quantitative Analysis --- p.136
Chapter 6.1 --- Introduction --- p.137
Chapter 6.1.1 --- Chemistry of quantitative PCR --- p.138
Chapter 6.1.2 --- PCR system --- p.140
Chapter 6.2 --- Materials & Methods --- p.142
Chapter 6.2.1 --- Design of primers and probes --- p.142
Chapter 6.2.2 --- Methods --- p.145
Chapter 6.3 --- Results --- p.146
Chapter 6.3.1 --- Selection of primer/probe --- p.146
Chapter 6.3.2 --- Primer optimization --- p.149
Chapter 6.3.3 --- Quantitative analysis of real samples --- p.158
Chapter 6.4 --- Discussion --- p.152
Chapter Chapter 7 --- Conclusion --- p.168
References --- p.175
Appendix --- p.193
Liou, Hang-Yu y 劉航宇. "Public Perceived Risk toward Genetically Modified Foods". Thesis, 2014. http://ndltd.ncl.edu.tw/handle/14407392675579131482.
Texto completo淡江大學
企業管理學系碩士班
102
The attitudes and perceived risk of GM foods toward people will affect it''s consumer behavior. To investigate the effects of perceived risks in GM food, most researchers use the gene technology acceptance model to measure the relationship of path analysis. More commonly used variables are the degree of trust and knowledge, and it have an impact on perceived risk and perceived benefits respectively, thereby affecting the attitude of GM. In this study, refer to this model and add a new dimension (intergenerational influence), the purpose is to understand the relationship about intergenerational influence, food neophobia, social trust, GM knowledge, perceived risk, perceived benefit and behavioral intentions. In this study, samples taken from Taiwan, we retrieved 423 valid samples. First we start pilot test to identify the item of reliability and validity, then using confirmatory factor analysis to determine the factor structure between variables and potential variables. In the end, we use path analysis to assess the relationship between variables of structural equation model. Our study developed a structural model ,and the results of structural equation modeling analysis give evidence that GM knowledge and intergenerational influence have positive impacts on the perceived risk,but social trust have negative impact on the perceived benefits. while the perceived risk and food neophobia have negative impacts on the behavioral intention,but the perceived benefits have positive impacts on the behavioral intention. The contribution of this study is that more understanding the mechanism between perception and behavior intention, and our study try to add a new dimension to probe the structural model, in addition, our study by clustering groups to understand the impact of differences. Our Study included management implications and recommends for manufacturers and government, as well as the future direction of research.
Catizone, Ilaria. "Public opinion on labelling of genetically modified foods". Thesis, 2005. http://hdl.handle.net/1885/151514.
Texto completoWang, Ching-Yi y 王晴怡. "Measuring Public Attitude of Genetically Modified Foods in Taiwan". Thesis, 2001. http://ndltd.ncl.edu.tw/handle/06447246777251908793.
Texto completo國立交通大學
經營管理研究所
89
Genetically modified foods have arisen many controversies in European and America. In Taiwan, public still has low awareness of these foods, but we can see food manufacturers label their foods as non-GM. The exposure information content will influence public attitude along with availability of other relevant reports. Public attitude will decide the direction of development of genetic engineering. The aim of this study is to understand public attitude formulation towards the new product, genetically modified foods. Based on the previous literature, Hoban, Woodrum and Czaja (1992) proposed awareness and the results indicated that people with low awareness have opposed attitude toward genetic engineering on food and agriculture. This study proposed prior exposure information content, a type of awareness, and interested in its influence on attitude. The results indicated that gender, prior exposure information content, and age are three predictors of attitude formulation. Therefore, we can conclude that (1) women have more opposed attitude than men have, (2) people with net positive prior exposure information content have greater positive attitude, (3) the young have greater positive attitude than the elder. All of these findings give important application. Women are the main consumers consuming foods; therefore, food manufacturers should pay attention on this result and their acceptability of genetically modified foods. While exposure information content become more and more available, public attitude will change; therefore, these transmitting information should be neutral to provide consumers to make subjective decisions. The young will be the major decision makers in the future. They have positive attitude may due to environmental consideration. Using genetic engineering may decrease the use of agriculture chemicals. Environmental preservation may be the most persuasive reason to persuade public to accept the genetically modified foods. Genetically modified foods cover many fields, including education, communication, and label. Education and communication will provide public knowledge to participate, and interact effectively. Public should be given rights to do consuming choice by labeling genetically modified foods.