Tesis sobre el tema "Genetic technology"
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Yu, Bin y 于斌. "Study of recombineering technology in Salmonella and its applications". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hdl.handle.net/10722/211555.
Texto completopublished_or_final_version
Biochemistry
Doctoral
Doctor of Philosophy
Hultin, Emilie. "Genetic Sequence Analysis by Microarray Technology". Doctoral thesis, Stockholm : School of Biotechnology, Royal Institute of Technology, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4330.
Texto completoLindroos, Katarina. "Accessing Genetic Variation by Microarray Technology". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2002. http://publications.uu.se/theses/91-554-5251-5/.
Texto completoFreethy, Randy J. "The ethics of genomic technology". Theological Research Exchange Network (TREN), 2005. http://www.tren.com/search.cfm?p001-1054.
Texto completoWeston, Delys E. "Democracy and political economy of genetic engineering /". Access via Murdoch University Digital Theses Project, 2007. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20070327.143205.
Texto completoNeadeau, Joseph Francis. "Comparing Genetic Modification and Genetic Editing Technolgies: Minimal Required Acreage". Thesis, North Dakota State University, 2018. https://hdl.handle.net/10365/29878.
Texto completoZhuang, Nan. "Logic synthesis and technology mapping using genetic algorithms". Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286760.
Texto completoAsadi, Romisa. "Development of genetic control technology for Tephritid pests". Thesis, Cardiff University, 2015. http://orca.cf.ac.uk/72611/.
Texto completoVeikondis, Rene. "Genetic characterisation of fungal disease resistance genes in grapevine using molecular marker technology". Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/96090.
Texto completoENGLISH ABSTRACT: The aim of this study on grapevine was to genetically characterise, validate and map the reported fungal disease resistance genes of Pölöskei Muskotály (PM), Kishmish Vatkana (KV) and Villard Blanc (VB) in South Africa using QTL analysis. These fungal resistant parents were crossed with other varieties that have desirable fruit qualities in an effort to combine fungal disease resistance with desirable fruit qualities in a single variety. The genetic basis of PM’s resistance to downy and powdery mildew has not been investigated before. It does however have VB in its pedigree so the assumption was made that the same QTL/genes present in VB contribute to this resistance. KV’s resistance to powdery mildew reportedly originates from the REN1 gene located on chromosome 13. VB’s powdery and downy mildew resistance is conferred by QTL present on chromosome 15 and chromosome 18 respectively and has been reported in numerous studies. The study populations comprised of 124 F1 PM x Regal Seedless plants, 16 F1 PM x G4-3418 plants, 14 F1 PM x Sunred Seedless plants, 158 F1 Sunred Seedless x KV plants and 250 F1 VB x G1-6604 plants. DNA was extracted from the leaves and all plants were screened using microsatellite markers. Phenotypic evaluations of downy and/or powdery mildew resistance were performed on the appropriate populations. The molecular data was used to generate linkage maps and combined with phenotypic data to perform QTL analysis. From the molecular data generated for the three PM populations it was determined that the F1 progeny inherited almost exclusively maternal alleles, and could not be used in a mapping study. These populations were eliminated from the study and PM will be used as a pollen donor in future. Molecular data from the Sunred Seedless x KV cross was used to generate a linkage map for chromosome 13 comprising eight markers and spanning 45.6 cM. When combined with the data from two powdery mildew phenotypic screens a QTL peak spanning the REN1 gene on chromosome 13 of KV was identified. This locus explains between 44.8% and 57.7% of the phenotypic variance observed. The molecular data from the VB x G1-6604 cross was used to generate partial linkage maps for chromosome 15 and 18. Eleven markers were mapped on chromosome 15 spanning 56.4 cM, and ten markers were mapped on chromosome 18 spanning 101.8 cM. When the chromosome 15 linkage map was combined with the data from two powdery mildew phenotypic screens a QTL associated with powdery mildew resistance was identified on chromosome 15 that explains between 18.9% and 23.9% of the phenotypic variance observed. Likewise a QTL associated with downy mildew resistance was identified on chromosome 18 when the chromosome 18 linkage map was combined with data from two downy mildew phenotypic screens. This QTL explains between 19.1% and 21.2% of the phenotypic variance observed. This study succeeded in genetically characterising the fungal disease resistance genes of two different sources of grapevine and provided exclusionary information on a third resistance source for future breeding applications.
AFRIKAANSE OPSOMMING: Die doel van hierdie studie in wingerd was om die genetiese komponent van die swamweerstandsgene van Pölöskei Muskotály (PM), Kishmish Vatkana (KV) and Villard Blanc (VB) in Suid-Afrika te karakteriseer en die teenwoordigheid daarvan te bevestig deur ʼn Kwantitatiewe Eienskap Lokus (KEL) benadering te volg. In ʼn poging om swamweerstand en goeie vrugeienskappe te kombineer in ʼn enkel variëteit is die weerstandige variëteite met vatbare variëteite gekruis wat goeie vrugeienskappe besit. Die genetiese basis van PM se weerstand teen donsskimmel en witroes is nog nie vantevore bestudeer nie. VB is een van sy voorgeslagte en daar is aangeneem dat dieselfde KEL/gene waarskynlik verantwoordelik is vir die weerstand. Dit is gerapporteer dat KV se witroesweerstand afkomstig is van die REN1 geen op chromosoom 13. Vele publikasies rapporteer VB se weerstand teen witroes en donsskimmel Beide die witroes- en donsskimmelweerstand word oorgedra deur KEL teenwoordig op chromosome 15 en 18 onderskeidelik. Die populasies gebruik in hierdie studie het bestaan uit 124 F1 PM x Regal Seedless plante, 16 F1 PM x G4-3418 plante, 14 F1 PM x Sunred Seedless, 158 F1 Sunred Seedless x KV plante en 250 F1 VB x G1-6604 plante onderskeidelik. Blare is versamel vir DNS isolasie en genotipering met mikrosatellietmerkers. Al drie populasies se weerstand teen donsskimmel en/of witroes is fenotipies geëvalueer. Die molekulêre data is gebruik om genetiese koppelingskaarte op te stel en gekombineer met die fenotipiese data om KEL analise uit te voer. Die molekulêre data van die drie PM populasies het daarop gedui dat die F1 nageslag amper uitsluitlik moederlike allele geërf het en kon gevolglik nie gebruik word in die studie nie. Die PM populasies is uitgesluit uit hierdie studie en PM sal voortaan as stuifmeelskenker gebruik word. Molekulêre data van die Sunred Seedless x KV kruising is gebruik om ʼn koppelingskaart vir chromosoom 13 op te stel wat 45.6 cM lank is en agt merkers bevat. Die KEL analise van die koppelingskaart en twee fenotipiese datastelle vir witroes het ʼn KEL piek geïdentifiseer wat oor die lengte van die REN1 geen-interval strek. Hierdie lokus is verantwoordelik vir 44.8% tot 57.7% van die fenotipiese variasie wat waargeneem word. Molekulêre data van die VB x G1-6604 kruising is gebruik om gedeeltelike koppelingskaarte vir chromosome 15 en 18 op te stel. Elf merkers karteer op die chromosoom 15 kaart van 56.4 cM en tien merkers karteer op die chromosoom 18 kaart van 101.8 cM. KEL analise van chromosoom 15 se koppelingskaart en twee witroes fenotipiese datastelle het ʼn KEL geïdentifiseer wat 18.9% tot 23.9% van die fenotipiese variasie verduidelik. ʼn KEL is ook op chromosoom 18 geïdentifiseer wat 19.1% tot 21.2% van die fenotipiese variasie verduidelik met die gekombineerde analise van chromosoom 18 se koppelingskaart en twee donsskimmel fenotipiese datastelle. Hierdie studie het die genetiese komponent van die swamweerstandsgene van twee Vitis variëteite suksesvol gekarakteriseer en bevestig. Waardevolle telingsinligting oor die derde variëteit is ook onthul.
Johansson, Magnus. "Financial application of genetic programming". Thesis, Linköping University, Department of Computer and Information Science, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-17091.
Texto completoJenner, Kris Harlan. "The study of inherited diseases using recombinant DNA technology". Thesis, University of Oxford, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670385.
Texto completoEsparcia, Alcázar Anna Isabel. "Genetic programming for adaptive digital signal processing". Thesis, University of Glasgow, 1998. http://theses.gla.ac.uk/4780/.
Texto completoRisely, Melissa. "The politics of precaution : an eco-political investigation of agricultural gene technology policy in Australia, 1992-2000". Title page, contents and abstract only, 2003. http://web4.library.adelaide.edu.au/theses/09PH/09phr5953.pdf.
Texto completoDixit, Atray (Atray Chitanya). "Methods for bounding genetic nonlinearities". Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/117897.
Texto completoCataloged from PDF version of thesis.
Includes bibliographical references.
Complex hierarchical structures are a hallmark of life. Within multicellular organisms, the building blocks of these structures are cells; within cells, they are genes. The interdependence of these building blocks is difficult to measure but is integral to the biological processes of health and disease, which emerge from the dynamism of thousands of interacting genes. This cooperativity manifests in particular mutations which accumulate over the course of cancer progression, gender-specific medical conditions, and transcription factor cocktails used to reprogram differentiated cells into stem cells. However, it is experimentally intractable to test the significance of perturbing every unique combination of genes. Instead, we explore gross features of this interaction space to determine how prevalent these synergies are. We take a top-down approach, creating new methods to measure the effects of removing genes from the full set. In the first, we develop a method to measure the transcriptional response to genetic perturbations across hundreds of thousands of cells revealing opposing classes of transcription factors regulating the immune response of dendritic cells. In the second, we create a method to measure how millions of combinations of genetic perturbations impact the growth rate of cancer cell lines.
by Atray Dixit.
Ph. D. in Medical Engineering and Medical Physics
Mansfield, Robert Patrick William. "Developments in genetic engineering of novel acetogens". Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/51833/.
Texto completoDahl, Fredrik. "Selector Technology : For Multiplex DNA Analysis". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5921.
Texto completoIsaksson, Magnus. "Extracting Genomic Variations using Selector Technology". Doctoral thesis, Uppsala universitet, Institutionen för genetik och patologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-121429.
Texto completoFredriksson, Mona. "Using Minisequencing Technology for Analysing Genetic Variation in DNA and RNA". Doctoral thesis, Uppsala University, Department of Medical Sciences, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4789.
Texto completoIn this thesis, the four-color fluorescence tag-microarray minisequencing system pioneered by our group was further developed and applied for analysing genetic variation in human DNA and RNA. A SNP marker panel representing different chromosomal regions was established and used for identification of informative SNP markers for monitoring chimerism after stem cell transplantation (SCT). The success of SCT was monitored by measuring the allelic ratios of informative SNPs in follow-up samples from nine patients with leukaemia. The results agreed with data obtained using microsatellite markers. Further the same SNP marker panel was used for evaluation of two whole genome amplification methods, primer extension preamplification (PEP) and multiple displacement amplification (MDA) in comparison with genomic DNA with respect to SNP genotyping success and accuracy in tag-array minisequencing. Identical results were obtained from MDA products and genomic DNA.
The tag-microarray minisequencing system was also established for multiplexed quantification of imbalanced expression of SNP alleles. Two endothelial cell lines and a panel of ten coding SNPs in five genes were used as model system. Six heterozygous SNPs were genotyped in RNA (cDNA) from the cell lines. Comparison of the relative amounts of the SNPs alleles in cDNA to heterozygote SNPs in genomic DNA displayed four SNPs with significant imbalanced expression between the SNP alleles. Finally, the tag-array minisequencing system was modified for detection of splice variants in mRNA from five leukaemia cell lines. A panel of 20 cancer-related genes with 74 alternatively splice variants was screened. Over half of the splice variants were detected in the cell lines, and similar alternative splicing patterns were observed in each cell line. The results were verified by size analysis of the PCR product subjected to the minisequencing primer extension reaction. The data from both methods agreed well, evidencing for a high sensitivity of our system.
Zhang, Zhifen. "Use of genetic transformation technology in oil crops: soybean and sunflower". The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1462871872.
Texto completoMoore, Heather Corrina. "Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology". Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/194108.
Texto completoHe, Huiqi. "Miniaturized electroporation system for gene transfer using bio-MEMS technology /". View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?BIEN%202007%20HE.
Texto completoEkström, Jens-Ola. "Algorithms for Aligning Genetic Sequences to Reference Genomes". Thesis, Umeå universitet, Institutionen för datavetenskap, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-142507.
Texto completoEmenheiser, Joseph Carl. "Use of ultrasound technology in the genetic improvement of U.S. lamb composition". Thesis, Virginia Tech, 2009. http://hdl.handle.net/10919/31178.
Texto completoMaster of Science
Oh, Jay J. "The Imago Dei and its implications for germ-line genetic enhancement technology". Theological Research Exchange Network (TREN), 1997. http://www.tren.com.
Texto completoRossi, Jairus. "Ecological Restoration's Genetic Culture: Participation and Technology in the Making of Landscapes". UKnowledge, 2013. https://uknowledge.uky.edu/geography_etds/15.
Texto completoYin, Guangyao. "Theoretical analysis and experiments of single cell electroporation using MEMS technology /". View abstract or full-text, 2010. http://library.ust.hk/cgi/db/thesis.pl?BIEN%202010%20YIN.
Texto completoChow, Rachel Anne. "The genetic characterization of populations comprising the Austronesian language family". FIU Digital Commons, 2004. http://digitalcommons.fiu.edu/etd/2349.
Texto completoThakur, Sanjay y n/a. "The ethics of preimplantation genetic diagnosis". University of Otago. Department of Philosophy, 2006. http://adt.otago.ac.nz./public/adt-NZDU20060816.105106.
Texto completoOhlin, Mats. "Human monoclonal antibody technology a tool to investigate human antibody repertoires /". Lund : Dept. of Immunotechnology, Lund University, 1992. http://catalog.hathitrust.org/api/volumes/oclc/39693827.html.
Texto completoMohan, Nisha. "Individualised modelling using transductive inference and genetic algorithms this thesis is presented as a part of the requirements for the award of the degree of Master of Information Technology at the Auckland University of Technology, June 2005". Full thesis. Abstract, 2005.
Buscar texto completoHuggins, Rachel. "Can genetic justice survive? : DNA technology and social control in the 21st century". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ54286.pdf.
Texto completoDahlgren, Andreas. "Analysis of Complex Genetic Traits in Population Cohorts using High-throughput Genotyping Technology". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8291.
Texto completoMiah, Andy. "Philosophical and ethical questions concerning technology in sport : the case of genetic modification". Thesis, De Montfort University, 2002. http://hdl.handle.net/2086/5205.
Texto completoJohnson, Richard. "Factors affecting intent to use consumer genetic tests : a revised technology acceptance model". Diss., University of Pretoria, 2010. http://hdl.handle.net/2263/24001.
Texto completoDissertation (MBA)--University of Pretoria, 2010.
Gordon Institute of Business Science (GIBS)
unrestricted
Liddell, Kathleen. "Biolaw and deliberative democracy : regulating human genetic technology in a morally pluralist society". Thesis, Oxford : Univ. of Oxford, Division of Social Sciences; Faculty of law, 2003. http://swbplus.bsz-bw.de/bsz118775707inh.htm.
Texto completoBevington, Linda K. "The creation of humankind in the image of God and the incarnation of Christ implications for human genetic engineering, reproductive technology, and cloning /". Theological Research Exchange Network (TREN), 1997. http://www.tren.com.
Texto completoOzbey, Halil. "A Genetic-based Intelligent Intrusion Detection System". Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/2/12606636/index.pdf.
Texto completos behavior in the absence of negative data. First, we design and develop an intelligent and behavior-based detection mechanism using genetic-based machine learning techniques with subsidies in the Bucket Brigade Algorithm. It classifies the possible system states to be normal and abnormal and interprets the abnormal state observations as evidences for the presence of an intrusion. Next we provide another algorithm which focuses on capturing normal behavior of the target system to detect intrusions again by identifying anomalies. A compact and highly complete rule set is generated by continuously inserting observed states as rules into the rule set and combining similar rule pairs in each step. Experiments conducted using the KDD-99 data set have produced fairly good results for both of the algorihtms.
Bentley, Patricia Peterson 1954. "Genetic manipulation : the paradox of control in a flexible corporation". Thesis, Massachusetts Institute of Technology, 2000. http://hdl.handle.net/1721.1/34340.
Texto completoIncludes bibliographical references (p. 399-410).
This dissertation is a two-theme ethnography focusing on the early history of one company within the context of the turbulent business environment of the 1990's. One theme is the control exercised by a corporation to mold its people to achieve certain productive ends, focusing on three areas: culture, physical environment and technology. The second theme is the ability of a corporation to be flexible. Taken together, the two themes form the self-contradictory notion of trying to control a group to increase its ability to be flexible. Many writers who focus on organizations have found the biological metaphor of evolution a useful way to conceptualize some aspects of a successful firm. In contrast I find the biological metaphor of genetic manipulation best illustrates the kind of control exercised by the leadership of this particular firm. From its inception, the leadership team wanted to create a flexible firm, one that could thrive in a turbulent environment. Rather than rely on a multiplicity of heterogeneous experiments, they actively manipulated specific aspects of the firm. The early results, the formation of a successful company, suggested that those controls and the decision to actively mold the firm using such controls were the right choices. When faced with a radical change in the marketplace, the arrival of the Internet economy, the leaders of this firm responded with the same technique and once again were able to mold a successful firm. To the extent that the Internet economy requires companies to change at Internet speed, this firm's ability to manipulate its own "DNA" may well be a model for success for other firms in this environment.
by Patricia Peterson Bentley.
Ph.D.
Baccare, Grace. "Genetic Enhancement, Hyperagency, and Humanity. An Investigation of the Implications". Thesis, Boston College, 2018. http://hdl.handle.net/2345/bc-ir:108028.
Texto completoThe genetic enhancement the human genome would be humanity’s most extreme attempt in the quest for hyperagency, and will have negative implications for our sense of humanity. Hyperagency is an extreme over-expression of our own human agency; everything is transparent, subject to our control and manipulation, and in accordance with our own interests. Modern era philosophical theories in subjectivity and agency have developed, evolved, and responded to advancements in science and technology over the past few centuries, and have all contributed to the current shift in understanding of our own humanity, influencing the rise of hyperagency in the postmodern world. The act of manipulating an organism’s genetic material for the purposes of changing and modifying its characteristics is referred to as genetic modification. The term genetic enhancement is more specifically indicative of the process of modifying nonpathological, or non-disease related genes. Genetic enhancement, in the form of germline engineering especially, exhibits a dangerous attitude of hyperagency that will have negative consequences for humanity as a whole. Hyperagency not only disrupts our sense of reverence before mystery and depth but also threatens our sense of morality in relating to the world. If continued, practices in hyperagency such as genetic enhancement will lead us to lose our sense of humanity altogether
Thesis (BA) — Boston College, 2018
Submitted to: Boston College. College of Arts and Sciences
Discipline: Departmental Honors
Discipline: Philosophy
Nebaeus, Tobias. "Optimal Scaling Configurations for Microservice-Oriented Architectures Using Genetic Algorithms". Thesis, Umeå universitet, Institutionen för datavetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-164765.
Texto completoGoodman, Daniel B. (Daniel Bryan). "Understanding genetic systems through multiplexed design, synthesis, and measurement". Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/104615.
Texto completoCataloged from PDF version of thesis.
Includes bibliographical references (pages 149-158).
Next-generation DNA sequencing has allowed us to extract vast quantities of functional information from genetic systems. However, natural systems represent only a fraction of all possible DNA sequences. Our understanding of how genomes function is limited by our ability to make modifications and test hypotheses. Multiplexed DNA synthesis now allows us to generate thousands of computationally designed sequences, each representing a physical hypothesis to test. Here, we combine DNA sequencing and synthesis technologies to design, make, and measure the behavior of thousands of new genetic elements in the bacterium E. coli. We begin by quantifying the interactions between regulatory elements that control transcription and translation and show that these interactions create large deviations from the predicted behavior of individual elements. Regulatory elements also interact with the codons of the genes they control. We show that rare codon usage at the beginning of genes unexpectedly leads to a strong increase in protein translation due to the relationship between codon rarity, genomic nucleotide bias, and mRNA structure. We next examine the behavior of regulatory elements that bind transcription factors by designing and synthesizing over 100,000 transcriptional circuits. From each circuit we measure repression, activation, and small-molecule induction, deriving relationships between DNA sequence features and functional properties including cooperativity, sensitivity, and dynamic range of gene expression response. Finally, as the scale and speed of DNA synthesis and functional readout continues to increase, our ability to computationally design and analyze genetic systems has become the bottleneck. We have built software to predict and design individual genetic elements in high throughput (Promuter) as well as software to analyze and compare hundreds of evolved or engineered bacterial whole genomes (Millstone). As generating high dimensional datasets becomes exponentially easier than designing experiments and extracting knowledge, bioinformatics, machine learning, and data science will become the primary tools we use to pose new hypotheses and build models of biology.
by Daniel B. Goodman.
Ph. D. in Bioinformatics and Integrative Genomics
Reynolds, David. "Theory of genetic algorithms with applications to heat integration networks". Thesis, Glasgow Caledonian University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296464.
Texto completoRequena, Osete Jordi. "Advancing induced pluripotent stem cell (iPSC) technology by assessing genetic instability and immune response". Doctoral thesis, Universitat de Barcelona, 2017. http://hdl.handle.net/10803/457970.
Texto completoLes cèl·lules mare pluripotents induïdes (iPSC) es poden derivar de cèl·lules somàtiques adultes mitjançant la reprogramació amb Oct4, Sox2, Klf4 i c-Myc. Les iPSC han donat lloc a una nova tecnologia per estudiar i tractar malalties humanes (Takahashi et al., 2007). No obstant, abans de la aplicació clínica de les iPSC, dos problemes principals han de ser adreçats: (i) Estabilitat genètica de les iPSC. (ii) Resposta immune de les cèl·lules derivades de iPSC. Per adreçar aquests dos qüestions cabdals, la missió principal d’aquest doctorat és avançar la tecnologia de les iPSC adreçant dos objectius. El primer, és la substitució de c-Myc per Ciclina D1 al còctel de reprogramació (Oct4, Sox2, Klf4 and c-Myc o Ciclina D1) i segon, estudiar la resposta immune de les cèl·lules derivades de iPSC. Hem escollit Ciclina D1 per substituir c-Myc atès a observacions prèvies que pot ser emprat per reprogramar (Edel et al., 2010) i donada la seva funció en reparació de l’ADN (Chalermrujinanant et al., 2016). Les iPSC reprogramades amb Ciclina D1 presenten una pluripotència similar a les reprogramades amb c-Myc, l’anàlisi en profunditat mostra però, que les iPSC reprogramades amb Cyclin D1 tenen una reduïda inestabilitat genètica adreçada per: (i) reducció en ruptures de doble cadena de DNA, (ii) major quantitat nuclear de la proteïna clau en la recombinació homòloga Rad51, (iii) reducció en senyals multitelomèriques (MTS) i (iv) reducció en la cinètica de creixement de teratomes in vivo, en comparació amb iPSC reprogramades amb c-Myc. A més a més, demostrem que les cèl·lules mare neuronals derivades d’aquestes iPSC son capaces de implantar-se exitosament, sobreviure a llarg termini i diferenciar a neurones madures sense evidències de patologia en un model de dany medul·lar. També hem realitzat un anàlisi del sistema immune innat i adaptatiu de cèl·lules humanes de la pell (nomenades F1) reprogramades a iPSC i comparades amb cèl·lules derivades de iPSC (nomenades F2). Hem descobert una isoforma curta del Toll-Like Receptor 3 (TLR3), essencial en el reconeixement de RNA de doble cadena d’origen víric, que està predominantment sobreexpresada en totes les cèl·lules derivades de iPSC analitzades i no trobat en cèl·lules endògenes. Nosaltres proposem un nou model per el qual la isoforma curta del TLR3 competeix amb la isoforma llarga wild type desestabilitzant el procés essencial de dimerització del TLR3.
Sanchez, Antequera Yolanda. "Magselectofection: A novel integrated technology of magnetic separation and genetic modification of target cells". Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-127469.
Texto completoPark, Nigel. "The application of Australian-developed performance and genetic technology to the Chinese beef industry". University of Southern Queensland, Faculty of Arts, 2003. http://eprints.usq.edu.au/archive/00001479/.
Texto completoSong, Simon Deping. "Use of Genetic Technology to Understand Ecological and Behavioural Strategies of Bactrocera cacuminata (Hering)". Thesis, Griffith University, 2009. http://hdl.handle.net/10072/366191.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environment
Science, Environment, Engineering and Technology
Full Text
Tariyal, Ridhi. "Finding utility for genetic diagnostics in the developing world". Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/63229.
Texto completoCataloged from PDF version of thesis.
Includes bibliographical references (p. 61-64).
Genetic testing companies have come under fire lately for an array of reasons. Many direct-to-consumer outfits are being challenged by the federal regulatory authorities, by the physicians' community and by the public itself. The desire to derive utility from the existing mass of genetic research is only outpaced by the sheer amount of new information being added to our understanding daily. These genetic testing companies are simultaneously trying to apply the existing knowledge, build a base for further study and be credible, going concerns from a business perspective. It is a worthy but difficult objective. The direct-to-consumer genetic initiatives face resistance from physicians who are the traditional intermediaries between medical insight and application of this insight. The companies also face a strong adversary in a government that wants to protect its constituents from fraudulent marketing claims and misinformation. Recent, informal studies have also exposed flaws in the product offerings and delivery of information by these companies. Finally, these are all for-profit entities which are struggling to become profitable. The objective of this thesis is to identify an attractive consumer base and opportunity that would allow for successful deployment of genetic diagnostic capability. I postulate that the success of a direct-to-consumer company would depend on finding a customer that values the genetic insight deeply and is able to take action from such insight. Based on those two fundamental criteria-perceived value and actionable utility-I build a profile of place, person and disease to test my hypothesis. Driven by the findings of my research, I anchored my hypothesis around an Indian consumer who pays for health care out-of-pocket, is vulnerable to certain genetic diseases due to narrow, endogamous customs and has grown up in a culture of arranged marriages. If this individual's religious and moral code forbids early termination of pregnancy or if financial and logistical circumstances make abortion impossible, I posit the desire for this cohort to use pre-marital genetic testing will increase. My research showed that people born in India and people who had considered arranged marriage as a viable option (the two groups overlapped but not completely) did display a greater likelihood of using genetic tests at the pre-marital and pre-natal stage to make informed decisions about family planning. These groups also showed a greater inclination towards early termination of pregnancy as well as reconsidering partner choice based on the outcome of genetic testing. However, the data also showed that those groups that did not believe in abortion still did not preferentially want a pre-marital genetic test.
by Ridhi Tariyal.
S.M.
Verster, Cornelis Thomas. "On supporting K-anonymisation and L-diversity of crime databases with genetic algorithms in a resource constrained environment". Master's thesis, University of Cape Town, 2015. http://hdl.handle.net/11427/20016.
Texto completoDinc, Mustafa. "Modeling Bluetooth radio technology simulation using Multi-agent based system and Genetic Algorithm design paradigm". Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2001. http://handle.dtic.mil/100.2/ADA391713.
Texto completoThesis advisors, John E. Hiles, Michael Zyda. Includes bibliographical references (p. 107-109). Also Available online.
Collins, Trevor. "The application of software visualization technology to evolutionary computation : a case study in Genetic Algorithms". Thesis, Open University, 1998. http://oro.open.ac.uk/28579/.
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